CN108478850A - A kind of regenerated silk fibroin hydrogel bandage and the preparation method and application thereof - Google Patents
A kind of regenerated silk fibroin hydrogel bandage and the preparation method and application thereof Download PDFInfo
- Publication number
- CN108478850A CN108478850A CN201810102492.3A CN201810102492A CN108478850A CN 108478850 A CN108478850 A CN 108478850A CN 201810102492 A CN201810102492 A CN 201810102492A CN 108478850 A CN108478850 A CN 108478850A
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- China
- Prior art keywords
- silk fibroin
- regenerated silk
- parts
- bandage
- fibroin hydrogel
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0052—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0076—Sprayable compositions
Abstract
The present invention provides a kind of regenerated silk fibroin hydrogel bandage, is made of the raw material of following proportioning：80 300 parts of 20 30 parts of regenerated silk fibroin powder, 10 20 parts of carbomer, 2 30 parts of triethanolamine, 10 30 parts of Pioloform, polyvinyl acetal, 38 parts of glycerine, 20 40 parts of ethyl alcohol, 25 parts of butyl p-hydroxybenzoate and deionized water.Described solid unit part is weight g；Liquid unit part is volume ml.Since the present invention is liquid gel bandage, there is tridimensional network, can be good at water lock, protect the microenvironment of the surface of a wound, protect granulation tissue, accelerating wound healing.Good airproof performance can completely cut off extraneous bacterium, dust.It is non-stimulated, without side-effects.Bandage of the present invention is in 60s or so fast filmings, i.e. spray is i.e. dry, easy to use.Preparation method of the present invention is simple, is suitable for industrialized production, there is larger application value.
Description
Technical field
Present document relates to biological medicines, and in particular to bandage and its preparation more particularly to a kind of New Regenerated fibroin albumen
Hydrogel bandage and the preparation method and application thereof.
Background technology
Regenerated silk fibroin is a kind of protein extracted from silk, the amino acid of needed by human body containing there are many, especially
It is human skin, the amino acid needed for hair.Fibroin albumen has good biocompatibility, can be used for preparing gel, film etc.
Material.Fibroin albumen has been widely studied and applied in field of biomedical materials at present, such as：Cell culture substrate, drug are slow
Controlled release and artificial organs etc..Lu Divine Land《The biology performance of fibroin material》In the detailed fibroin albumen of elaborating
Characteristic：With good biocompatibility, biological degradability, the regenerated ability of induction of vascular and film forming.
There are some apparent defects for traditional bandage, and e.g., poor sealing cannot completely cut off germ, Mou Xiete completely
Positioning is set inconvenient to use etc..In view of the foregoing drawbacks with problem, some liquid-type bandages have now been developed.This kind of wound
Patch is and in order to ensure rate of drying, to be also added into a large amount of organic solvent using artificial synthesized macromolecule as filmogen.Cause
The wound posts following point：Irritation is big, making material does not have self degradation function, wound surface and the external world can not gas-liquid
It exchanges, aeration difference etc. influences wound healing instead.Therefore, such as how fibroin albumen is main matrix, develops one kind and is efficiently cured
Conjunction, barrier germ, self degradation bandage without side-effects are a problem to be solved.
Invention content
It is with regenerated silk fibroin technical problem to be solved by the present invention lies in above-mentioned shortcoming, research and design is overcome
The bandage of main material.
The present invention provides a kind of regenerated silk fibroin hydrogel bandages.
Regenerated silk fibroin hydrogel bandage raw material is made：Regenerated silk fibroin powder, triethanolamine, gathers carbomer
Vinyl acetal, glycerine, ethyl alcohol, butyl p-hydroxybenzoate and deionized water.
Hydrogel bandage described herein refer to hydrogel produced by the present invention be applied to the surface of any object moment i.e.
The film layer that tight closure can be formed generates the protective layer of bandage effect if using in the wound surface of skin.
Specifically, a kind of regenerated silk fibroin hydrogel bandage provided by the invention is made of the raw material of following proportioning
The solid component is regenerated silk fibroin powder, carbomer, Pioloform, polyvinyl acetal, butyl p-hydroxybenzoate；
The liquid component is triethanolamine, glycerine, ethyl alcohol, deionized water.
Described solid unit part is weight；(g) liquid unit part is volume (ml).
Preferably, a kind of regenerated silk hydrogel bandage provided by the invention is made of the raw material of following proportioning
It is a further object of the present invention to provide the preparation method of the regenerated silk fibroin hydrogel bandage, this method
Include the following steps：
Regenerated silk fibroin powder, carbomer, Pioloform, polyvinyl acetal, ethyl alcohol and deionized water are mixed, in 50-80 DEG C of water
Heating stirring in bath measures pH value after all dissolving, and carries out being neutralized to pH value being 6.7-7.2 adjustment stirrings with triethanolamine
Speed 300-400rpm stirs 5h, and glycerine, butyl p-hydroxybenzoate is then added, continues to stir 12h, regenerated silk is made
Fibroin hydrogel, namely regenerated silk fibroin hydrogel bandage.
Raw material regenerated silk fibroin of the present invention is prepared by following method：
Raw material：Mulberry cocoon is provided by Te Si Bioisystech Co., Ltd of Huzhou Australia, and NaSCN (sodium sulfocynanate) is tried by Aladdin
Agent Co., Ltd provides, bag filter (molecular cut off 8000-14000, wide 10mm).
Preparation method：10g mulberry cocoons are dissolved in the NaSCN aqueous solutions that 40g mass fractions are 60%, are put into 65 DEG C of perseverances
1h is vibrated in warm incubator.Dissolving 0.5h is stood at the same temperature, and dialyse 3d.A deionized water is replaced every 12h, 50
DEG C vacuum drying for 24 hours, obtain regenerated silk fibroin powder 3.8g.
The present invention is then uniform by regenerated silk fibroin hydrogel bandage in use, need that the surface of a wound is first made debridement treatment
It is a small amount of to be applied to the surface of a wound, it can freely activity after waiting for 1 minute.
Regenerated silk fibroin hydrogel bandage provided by the present invention has following features：
(1) since the present invention is liquid bandage, carbomer and Pioloform, polyvinyl acetal are mixed together with regenerated silk fibroin
Closing use can further accelerated material form a film, and is prepared with absorption auxiliary material is promoted, and have tridimensional network, can be fine
Water lock, protect the microenvironment of the surface of a wound, protect granulation tissue, accelerating wound healing.
(2) bandage good airproof performance of the present invention, can completely cut off extraneous bacterium, dust.
(3) bandage good permeability of the present invention：Regenerated silk fibroin of the present invention can form three-dimensional network structure, have saturating
The good feature of the property of gas
(4) bandage of the present invention is non-stimulated, without side-effects.
(5) bandage of the present invention sprays i.e. dry in 60s or so fast filmings, i.e., easy to use.
Preparation method of the present invention is simple, is suitable for industrialized production, there is larger application value.
Specific implementation mode
Following embodiment raw materials and reagents unless otherwise indicated, are available.
Embodiment 1：
The preparation of regenerated silk fibroin：
Mulberry cocoon is provided by Te Si Bioisystech Co., Ltd of Huzhou Australia, and NaSCN (sodium sulfocynanate) is had by Aladdin reagent
Limit company provides, bag filter (molecular cut off 8000-14000, wide 10mm).
10g fibroin fibers (mulberry cocoon) are dissolved in the NaSCN aqueous solutions that 40g mass fractions are 60%, are put into
1h is vibrated in 65 DEG C of constant incubators.Dissolving 0.5h is stood at the same temperature, and dialyse 3d.A deionization is replaced every 12h
Water.Vacuum (95.4Kpa) is dry for 24 hours at 50 DEG C, and standard identification (standard fibroin is carried out with picric acid-famille rose identification reagent
Albumen is purchased in Beijing standard reagent), obtain regenerated silk fibroin powder 3.8g.
Embodiment 2：Raw material proportioning：
Specific preparation method：First 60ml deionized waters and 6ml ethyl alcohol are poured into the agitator tank of 200ml, is heated to 50 DEG C.
Then 3g regenerated silk fibroins powder, 2g carbomers, 3g Pioloform, polyvinyl acetals are poured into agitator tank, using stirring (200 turns/
Minute) persistently stir 2h.0.5ml butyl p-hydroxybenzoates, 0.2ml glycerine are dissolved in the deionized water of 36ml by period
In, for future use.Then adjust 500 revs/min of rotating speed, be slowly added dropwise into beaker triethanolamine to PH be 6.9 (triethanolamines
Dosage is 2ml), it is then slowly added into deployed butyl p-hydroxybenzoate, glycerine dissolving mixed solution.Continue to stir
5h is prepared into gel (being hydrogel bandage).
The above-mentioned gel prepared is uniformly applied on a small quantity on the slide of imitative human epidermal temperature, moment can be formed
Film.
Embodiment 3
Preparation method is the same as embodiment 2.
Embodiment 4
Preparation method is the same as embodiment 2.
Embodiment 5
Preparation method is the same as embodiment 2.
Embodiment 6
Preparation method is the same as embodiment 2.
Embodiment 7
Preparation method is the same as embodiment 2.
Embodiment 8
Preparation method is the same as embodiment 2.
Embodiment 9
Preparation method is the same as embodiment 2.
Embodiment 10
Preparation method is the same as embodiment 2.
Embodiment 11
Preparation method is the same as embodiment 2.
Embodiment 12：
It simulates human skin environment (about 30 DEG C of temperature) and carries out regenerated silk fibroin hydrogel film forming experiment (being shown in Table 1)
Table 1
| Group | Film formation time |
| Embodiment 2 | 56s |
| Embodiment 3 | 129s |
| Embodiment 4 | 97s |
| Embodiment 5 | 125s |
| Embodiment 6 | 68s |
| Embodiment 7 | 65s |
| Embodiment 8 | 115s |
| Embodiment 9 | 126s |
| Embodiment 10 | 138s |
According to above-mentioned experiment, illustrate that regenerated silk fibroin hydrogel film formation time is short, usually between 50-140 seconds.According to
The embodiment of said ratio adjusts data, and the proportioning of embodiment 2 is optimal.
Embodiment 13
Acute irritation test is tested
Before experiment about for 24 hours, family's rabbit back backbone diamond wool is cut, can not damages epidermis, unhairing range is left and right respectively about
3cm×3cm。
Table 2
Tested material sample is taken to select the product 0.5mL (g) of embodiment 2；Reference substance is that distilled water 0.5ml is coated directly on skin
On, it is then covered with two layers of gauze (2.5cm × 2.5cm) and one layer of glassine paper or the like, then with nonirritant adhesive plaster and stretch tight
Band is fixed.Other side skin is as a contrast.Using blocking test, the application time is 4h.Warm water or nothing are used after the test
Irritation solvent cleaning remains tested material, the results are shown in Table 2.
Test result illustrates that regenerated silk fibroin hydrogel of the present invention is nonirritant, safe to use.
Claims (4)
1. a kind of regenerated silk fibroin hydrogel bandage, which is characterized in that the regenerated silk fibroin hydrogel bandage by
The raw material of following proportioning is made
The solid component is regenerated silk fibroin powder, carbomer, Pioloform, polyvinyl acetal, butyl p-hydroxybenzoate；Liquid
Group is divided into triethanolamine, glycerine, ethyl alcohol, deionized water；
Described solid unit part is weight g；Liquid unit part is volume ml.
2. a kind of regenerated silk fibroin hydrogel bandage, which is characterized in that the regenerated silk fibroin hydrogel bandage by
The raw material of following proportioning is made
3. a kind of preparation method of regenerated silk fibroin hydrogel bandage as claimed in claim 1 or 2, which is characterized in that
This approach includes the following steps：
Regenerated silk fibroin powder, carbomer, Pioloform, polyvinyl acetal, ethyl alcohol and deionized water are mixed, in 50-80 DEG C of water-bath
Heating stirring measures pH value after all dissolving, and carries out being neutralized to pH value being that 6.7-7.2 adjusts mixing speed with triethanolamine
300-400rpm stirs 5h, and glycerine, butyl p-hydroxybenzoate is then added, continues to stir 12h, regenerated silk egg is made
Plain boiled water gel, namely regenerated silk fibroin hydrogel bandage.
4. regenerated silk fibroin hydrogel bandage according to claim 1 or 2, which is characterized in that the raw material regeneration
Fibroin albumen is prepared by following method：
10g mulberry cocoons are dissolved in the NaSCN aqueous solutions that 40g mass fractions are 60%, are put into 65 DEG C of constant incubators and shake
Swing 1h.Dissolving 0.5h is stood at the same temperature, and dialyse 3d, and a deionized water is replaced every 12h, is dried in vacuo at 50 DEG C
For 24 hours, regenerated silk fibroin powder 3.8g is obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810102492.3A CN108478850A (en) | 2018-02-01 | 2018-02-01 | A kind of regenerated silk fibroin hydrogel bandage and the preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810102492.3A CN108478850A (en) | 2018-02-01 | 2018-02-01 | A kind of regenerated silk fibroin hydrogel bandage and the preparation method and application thereof |
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| CN108478850A true CN108478850A (en) | 2018-09-04 |
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ID=63344397
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1348820A (en) * | 2001-11-10 | 2002-05-15 | 苏州大学 | Coremia-resisting wound protecting film and its prepn |
| CN102167724A (en) * | 2011-01-04 | 2011-08-31 | 复旦大学 | Controllable preparation method for high-concentration regenerated silk protein aqueous solution |
| CN103463669A (en) * | 2013-09-16 | 2013-12-25 | 重庆海默尼生物技术有限公司 | Liquid wound dressing and preparation method thereof |
| CN105477341A (en) * | 2015-12-16 | 2016-04-13 | 成都中医药大学 | Liquid adhesive bandage and preparation method thereof |
| CN107007876A (en) * | 2017-06-02 | 2017-08-04 | 江苏昌吉永生物科技股份有限公司 | Hyaluronic acid medical aseptic dressing and preparation method thereof |
-
2018
- 2018-02-01 CN CN201810102492.3A patent/CN108478850A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1348820A (en) * | 2001-11-10 | 2002-05-15 | 苏州大学 | Coremia-resisting wound protecting film and its prepn |
| CN102167724A (en) * | 2011-01-04 | 2011-08-31 | 复旦大学 | Controllable preparation method for high-concentration regenerated silk protein aqueous solution |
| CN103463669A (en) * | 2013-09-16 | 2013-12-25 | 重庆海默尼生物技术有限公司 | Liquid wound dressing and preparation method thereof |
| CN105477341A (en) * | 2015-12-16 | 2016-04-13 | 成都中医药大学 | Liquid adhesive bandage and preparation method thereof |
| CN107007876A (en) * | 2017-06-02 | 2017-08-04 | 江苏昌吉永生物科技股份有限公司 | Hyaluronic acid medical aseptic dressing and preparation method thereof |
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| TA01 | Transfer of patent application right |
Effective date of registration: 20181228 Address after: 264670 No. 11 Weiwu Road, Yantai High-tech Zone, Shandong Province Applicant after: Yantai Saida Medical Technology Co., Ltd. Address before: 225300 Room 1610, C1 Building, No. 1 Yaocheng Avenue, Taizhou City, Jiangsu Province Applicant before: Shuhe medical technology Jiangsu Co., Ltd. |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180904 |
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| WD01 | Invention patent application deemed withdrawn after publication |