CN108477488A - The production technology of low citrinin red yeast rice - Google Patents
The production technology of low citrinin red yeast rice Download PDFInfo
- Publication number
- CN108477488A CN108477488A CN201810287424.9A CN201810287424A CN108477488A CN 108477488 A CN108477488 A CN 108477488A CN 201810287424 A CN201810287424 A CN 201810287424A CN 108477488 A CN108477488 A CN 108477488A
- Authority
- CN
- China
- Prior art keywords
- rice
- red yeast
- monascus
- yeast rice
- long
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229940026314 red yeast rice Drugs 0.000 title claims abstract description 75
- CQIUKKVOEOPUDV-IYSWYEEDSA-N antimycin Chemical compound OC1=C(C(O)=O)C(=O)C(C)=C2[C@H](C)[C@@H](C)OC=C21 CQIUKKVOEOPUDV-IYSWYEEDSA-N 0.000 title claims abstract description 23
- CQIUKKVOEOPUDV-UHFFFAOYSA-N citrinine Natural products OC1=C(C(O)=O)C(=O)C(C)=C2C(C)C(C)OC=C21 CQIUKKVOEOPUDV-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 238000005516 engineering process Methods 0.000 title claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 241000209094 Oryza Species 0.000 claims abstract description 70
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 70
- 235000009566 rice Nutrition 0.000 claims abstract description 70
- 241000228347 Monascus <ascomycete fungus> Species 0.000 claims abstract description 66
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 61
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000001963 growth medium Substances 0.000 claims abstract description 32
- 235000013339 cereals Nutrition 0.000 claims abstract description 22
- 238000005406 washing Methods 0.000 claims abstract description 21
- 238000001035 drying Methods 0.000 claims abstract description 17
- 238000011534 incubation Methods 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims description 41
- 229960002163 hydrogen peroxide Drugs 0.000 claims description 28
- 239000000725 suspension Substances 0.000 claims description 15
- 239000002609 medium Substances 0.000 claims description 13
- 238000011081 inoculation Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 244000061458 Solanum melongena Species 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 235000020195 rice milk Nutrition 0.000 claims description 5
- 239000003643 water by type Substances 0.000 claims description 5
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 3
- 229910052739 hydrogen Inorganic materials 0.000 claims description 3
- 239000001257 hydrogen Substances 0.000 claims description 3
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 claims 1
- 241000894006 Bacteria Species 0.000 description 6
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 244000113306 Monascus purpureus Species 0.000 description 2
- 235000002322 Monascus purpureus Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229940057059 monascus purpureus Drugs 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Polymers & Plastics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of production technologies of low citrinin red yeast rice, and monascus thalline is inoculated in long-grained nonglutinous rice culture medium and carries out grain of rice Spawn incubation to obtain red yeast rice;Then, hydrogen peroxide washing and drying are first carried out, then is irradiated;Alternatively, first being irradiated, then carry out hydrogen peroxide washing and drying;To obtain finished product;The irradiation dose is 7kGy 8kGy.The purpose of the present invention is to provide a kind of production technologies of low citrinin red yeast rice.
Description
Technical field
The present invention relates to a kind of production technologies of low citrinin red yeast rice.
Background technology
Citrinin poisons with kidney and with carcinogenicity, however it is the intrinsic secondary metabolite of Monascus.Often
Citrinin can be metabolized by advising in the preparation process of red yeast rice, this is the serious obstruction for ensureing red yeast rice safety.
Invention content
The purpose of the present invention is to provide a kind of production technologies of low citrinin red yeast rice.
The purpose of the present invention is achieved through the following technical solutions:A kind of production technology of low citrinin red yeast rice, by red yeast rice
Mould body, which is inoculated in long-grained nonglutinous rice culture medium, carries out grain of rice Spawn incubation to obtain red yeast rice;
Then, hydrogen peroxide washing and drying are first carried out, then is irradiated;Alternatively, first being irradiated, then carry out peroxidating
Hydrogen is washed and is dried;
To obtain finished product;The irradiation dose is 7kGy-8kGy.
For the prior art, the advantage of the invention is that:Red yeast rice culture is carried out using long-grained nonglutinous rice, and with oxidation and spoke
According to combined treatment, technique is relatively simple, efficient, under the premise of ensureing red yeast rice quality (keeping haematochrome to greatest extent),
The citrinin of 80%-90% is effectively removed, ensures red yeast rice safety;The highest color value 4500-5500 of finished product red yeast rice;Red yeast rice yield
35%-40%, citrinin content (in terms of unit color value) are less than 0.04mg/U.
Specific implementation mode
The content of present invention is described in detail with reference to embodiment:
Monascus thalline is inoculated with progress grain of rice bacterium in long-grained nonglutinous rice culture medium by a kind of production technology of low citrinin red yeast rice
Kind culture is to obtain red yeast rice;
Then, hydrogen peroxide washing and drying are first carried out, then is irradiated;Alternatively, first being irradiated, then carry out peroxidating
Hydrogen is washed and is dried;
To obtain finished product;The irradiation dose is 7kGy-8kGy.
The cultural method of the monascus thalline is,
It is prepared by a slant mediums:Brewer's wort 10oB é, are added 2% agar, and pH is adjusted to 6-7, dispenses, goes out after heating for dissolving
Bacterium tiltedly puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus (Monascus) spore suspension is inoculated in gnotobasis and is made into through step a
Slant medium in, the volume ratio of slant medium and Monascus spore suspension is 1:(0.032-0.06);The red yeast rice
The Monascus spore a concentration of 1.5 × 10 of mould spore suspension7A/mL-2.5 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, in 28 DEG C -30 DEG C of temperature
Middle culture -11 days 9 days, until inclined-plane is in integrally aubergine.
The specific method of the grain of rice Spawn incubation is,
It is prepared by d long-grained nonglutinous rice culture mediums:500 grams of -600 grams of long-grained nonglutinous rices are put into every 1 liter of water, are impregnated -30 hours 20 hours after stirring evenly,
Clear water is carried out later to rinse to no rice milk, then is drained to without dripping, and sterilizing in culture vessel is finally packed into;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through long-grained nonglutinous rice culture medium made from step d in gnotobasis
In be sufficiently mixed uniformly;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for that rice grain is in aubergine
.
The specific method of the monascus thalline inoculation is, to the inclined-plane culture after monascus thalline culture of every 1mL
1mL-2.5mL sterile waters are added in base and stir evenly, red yeast rice seed is made;Then red yeast rice seed is drawn to be added in long-grained nonglutinous rice culture medium simultaneously
It is sufficiently mixed uniformly, the long-grained nonglutinous rice before 1mL red yeast rice seed is impregnated with 30g-40g carries out inoculation proportioning.
The specific method of the red yeast rice culture is first to shake long-grained nonglutinous rice culture medium to one in 25 DEG C of -35 DEG C of incubators
Long-grained nonglutinous rice culture medium is shaken up and is divided out to continue heat insulating culture, daily shaking flask 2 by heap, heat insulating culture -30 hours 24 hours later
Secondary -3 times, be in aubergine through -7 days 5 days rice grains.
The specific method of hydrogen peroxide washing is, will treated that red yeast rice is put into concentration 0.8%- through previous process
It is rinsed in 1.2% hydrogenperoxide steam generator, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:(1.5-3), fully shakes
After even -30 minutes 20 minutes, drains to nothing and drip.
The method of the drying is that the red yeast rice after hydrogen peroxide carrying out washing treatment is put into insulating box, in 50 DEG C -70
DEG C temperature environment under, it is dry to moisture be 12% or less.
The method of the irradiation is, will treated that red yeast rice is irradiated using Co 60 through previous process.
It can be obtained finished product after irradiation.
In the present invention, the preferred monascus purpureus of monascus (Monascus purpureus);The method of the sterilizing can
To be in 0.08MPa-0.13MPa high pressure steam sterilizations -30 minutes 25 minutes;The gnotobasis can be first 2 hours of inoculation
Ultraviolet disinfection is carried out to reach the environment of Sterile standard.
Embodiment 1:
Monascus thalline is inoculated with progress grain of rice bacterium in long-grained nonglutinous rice culture medium by a kind of production technology of low citrinin red yeast rice
Then kind culture carries out hydrogen peroxide washing and drying to obtain red yeast rice, finally irradiate and obtain finished product;The irradiation dose is
7kGy。
The cultural method of the monascus thalline is,
It is prepared by a slant mediums:Brewer's wort 10o2% agar is added in B é, and pH is adjusted to 6, is dispensed after heating for dissolving, sterilizing,
It tiltedly puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus spore suspension is inoculated with through inclined-plane culture made from step a in gnotobasis
In base, the volume ratio of slant medium and Monascus spore suspension is 1:0.06;The red yeast rice of the Monascus spore suspension
Mould spore concentration is 1.5 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, is trained in 28 DEG C of temperature
It supports 11 days.
The specific method of the grain of rice Spawn incubation is,
It is prepared by d long-grained nonglutinous rice culture mediums:500 grams of long-grained nonglutinous rices are put into every 1 liter of water, is impregnated 20 hours after stirring evenly, carries out clear water later
It rinses to no rice milk, then drains to without dripping, be finally packed into sterilizing in culture vessel;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through long-grained nonglutinous rice culture medium made from step d in gnotobasis
In be sufficiently mixed uniformly;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for that rice grain is in aubergine
.
The specific method of the monascus thalline inoculation is, to the inclined-plane culture after monascus thalline culture of every 1mL
2.5mL sterile waters are added in base and stir evenly, red yeast rice seed is made;Then red yeast rice seed is drawn to be added in long-grained nonglutinous rice culture medium simultaneously fully
It is uniformly mixed, the long-grained nonglutinous rice before 1mL red yeast rice seed is impregnated with 30g carries out inoculation proportioning.
The specific method of the red yeast rice culture is first to shake long-grained nonglutinous rice culture medium to a pile in 25 DEG C of incubators, protect
Temperature culture 30 hours, long-grained nonglutinous rice culture medium is shaken up and divided out later to continue heat insulating culture 7 days, daily shaking flask 2 times.
The specific method of the hydrogen peroxide washing is that the red yeast rice obtained through grain of rice Spawn incubation is put into concentration
It is rinsed in 1.2% hydrogenperoxide steam generator, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:3, fully shake up 20 points
Zhong Hou is drained to nothing and is dripped.
The method of the drying is that the red yeast rice after hydrogen peroxide carrying out washing treatment is put into insulating box, in 50 DEG C
Under temperature environment, drying to moisture is 12% or less.
The method of the irradiation is, will be oxidation-treated after red yeast rice irradiated using Co 60.
It can be obtained finished product after irradiation.
Embodiment 2:
Monascus thalline is inoculated with progress grain of rice bacterium in long-grained nonglutinous rice culture medium by a kind of production technology of low citrinin red yeast rice
Then kind culture carries out hydrogen peroxide washing and drying to obtain red yeast rice, finally irradiate and obtain finished product;The irradiation dose is
7.5kGy。
The cultural method of the monascus thalline is,
It is prepared by a slant mediums:Brewer's wort 10oB é, are added 2% agar, and pH is adjusted to 6.5, dispenses, goes out after heating for dissolving
Bacterium tiltedly puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus spore suspension is inoculated with through inclined-plane culture made from step a in gnotobasis
In base, the volume ratio of slant medium and Monascus spore suspension is 1:0.05;The red yeast rice of the Monascus spore suspension
Mould spore concentration is 2.0 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, is trained in 29 DEG C of temperature
It supports 10 days.
The specific method of the grain of rice Spawn incubation is,
It is prepared by d long-grained nonglutinous rice culture mediums:585 grams of long-grained nonglutinous rices are put into every 1 liter of water, is impregnated 24 hours after stirring evenly, carries out clear water later
It rinses to no rice milk, then drains to without dripping, be finally packed into sterilizing in culture vessel;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through long-grained nonglutinous rice culture medium made from step d in gnotobasis
In be sufficiently mixed uniformly;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for that rice grain is in aubergine
.
The specific method of the monascus thalline inoculation is, to the inclined-plane culture after monascus thalline culture of every 1mL
2mL sterile waters are added in base and stir evenly, red yeast rice seed is made;Then red yeast rice seed is drawn to be added in long-grained nonglutinous rice culture medium and fully mix
It closes uniformly, the long-grained nonglutinous rice before 1mL red yeast rice seed is impregnated with 36g carries out inoculation proportioning.
The specific method of the red yeast rice culture is first to shake long-grained nonglutinous rice culture medium to a pile in 30 DEG C of incubators, protect
Temperature culture 27 hours, long-grained nonglutinous rice culture medium is shaken up and divided out later to continue heat insulating culture 6.5 days, daily shaking flask 3 times.
The specific method of the hydrogen peroxide washing is that the red yeast rice obtained through grain of rice Spawn incubation is put into concentration 1%
Hydrogenperoxide steam generator in rinsed, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:2, after fully shaking up 30 minutes,
It drains to nothing and drips.
The method of the drying is that the red yeast rice after hydrogen peroxide carrying out washing treatment is put into insulating box, in 60 DEG C
Under temperature environment, drying to moisture is 12% or less.
The method of the irradiation is, will be oxidation-treated after red yeast rice irradiated using Co 60.
It can be obtained finished product after irradiation.
Embodiment 3:
Monascus thalline is inoculated with progress grain of rice bacterium in long-grained nonglutinous rice culture medium by a kind of production technology of low citrinin red yeast rice
Then kind culture carries out hydrogen peroxide washing and drying to obtain red yeast rice, finally irradiate and obtain finished product;The irradiation dose is
8kGy。
The cultural method of the monascus thalline is,
It is prepared by a slant mediums:Brewer's wort 10o2% agar is added in B é, and pH is adjusted to 7, is dispensed after heating for dissolving, sterilizing,
It tiltedly puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus spore suspension is inoculated with through inclined-plane culture made from step a in gnotobasis
In base, the volume ratio of slant medium and Monascus spore suspension is 1:0.032;The red yeast rice of the Monascus spore suspension
Mould spore concentration is 2.5 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, is trained in 30 DEG C of temperature
It supports 9 days.
The specific method of the grain of rice Spawn incubation is,
It is prepared by d long-grained nonglutinous rice culture mediums:600 grams of long-grained nonglutinous rices are put into every 1 liter of water, is impregnated 30 hours after stirring evenly, carries out clear water later
It rinses to no rice milk, then drains to without dripping, be finally packed into sterilizing in culture vessel;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through long-grained nonglutinous rice culture medium made from step d in gnotobasis
In be sufficiently mixed uniformly;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for that rice grain is in aubergine
.
The specific method of the monascus thalline inoculation is, to the inclined-plane culture after monascus thalline culture of every 1mL
1mL sterile waters are added in base and stir evenly, red yeast rice seed is made;Then red yeast rice seed is drawn to be added in long-grained nonglutinous rice culture medium and fully mix
It closes uniformly, the long-grained nonglutinous rice before 1mL red yeast rice seed is impregnated with 40g carries out inoculation proportioning.
The specific method of the red yeast rice culture is first to shake long-grained nonglutinous rice culture medium to a pile in 35 DEG C of incubators, protect
Temperature culture 24 hours, long-grained nonglutinous rice culture medium is shaken up and divided out later to continue heat insulating culture 5 days, daily shaking flask 3 times.
The specific method of the hydrogen peroxide washing is that the red yeast rice obtained through grain of rice Spawn incubation is put into concentration
It is rinsed in 0.8% hydrogenperoxide steam generator, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:1.5, fully shake up 27
After minute, drains to nothing and drip.
The method of the drying is that the red yeast rice after hydrogen peroxide carrying out washing treatment is put into insulating box, in 70 DEG C
Under temperature environment, drying to moisture is 12% or less.
The method of the irradiation is, will be oxidation-treated after red yeast rice irradiated using Co 60.
It can be obtained finished product after irradiation.
Embodiment 4:
This example the difference from example 2 is that:In this example, irradiation steps are first carried out, then carry out hydrogen peroxide washing step,
Step is finally dried.
Comparative example 1:
This example the difference from example 2 is that:In this example in hydrogen peroxide washing step, be used only etc. weight distilled water
It is rinsed;And there is no irradiation steps in this example.
Comparative example 2:
This example the difference from example 2 is that:There is no irradiation steps in this example.
Comparative example 3:
This example the difference from example 2 is that:There is no hydrogen peroxide washing step in this example.
In upper table, the measurement unit of citrinin content (in terms of unit color value) is mg/U.
Claims (7)
1. a kind of production technology of low citrinin red yeast rice, it is characterised in that:Monascus thalline is inoculated in long-grained nonglutinous rice culture medium
Grain of rice Spawn incubation is carried out to obtain red yeast rice;
Then, hydrogen peroxide washing and drying are first carried out, then is irradiated;Alternatively, first being irradiated, then carries out hydrogen peroxide and wash
It washs and dries;
To obtain finished product;The irradiation dose is 7kGy-8kGy.
2. the production technology of low citrinin Monascus color according to claim 1, it is characterised in that:The monascus thalline
Cultural method is,
It is prepared by a slant mediums:10 ° of B é of brewer's wort, are added 2% agar, and pH is adjusted to 6-7, is dispensed after heating for dissolving, sterilizing, tiltedly
It puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus spore suspension is inoculated with through slant medium made from step a in gnotobasis
In, the volume ratio of slant medium and Monascus spore suspension is 1:(0.032-0.06);The Monascus spore suspension
Monascus spore a concentration of 1.5 × 107A/mL-2.5 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, is trained in 28 DEG C -30 DEG C of temperature
It supports -11 days 9 days, until inclined-plane is in integrally aubergine.
3. according to the production technology of low citrinin Monascus color described in claim 1, it is characterised in that:The grain of rice Spawn incubation
Specific method is,
It is prepared by d long-grained nonglutinous rice culture mediums:500 grams of -600 grams of long-grained nonglutinous rices are put into every 1 liter of water, impregnate -30 hours 20 hours after stirring evenly, later
It carries out clear water to rinse to no rice milk, then drains to without dripping, be finally packed into sterilizing in culture vessel;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through being filled in long-grained nonglutinous rice culture medium made from step d in gnotobasis
Divide and is uniformly mixed;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for rice grain in aubergine.
The specific method of monascus thalline inoculation is,
1mL-2.5mL sterile waters are added into the slant medium after monascus thalline culture of every 1mL and stir evenly, are made red
Sort of quyi;Then it draws red yeast rice seed to be added in long-grained nonglutinous rice culture medium and be sufficiently mixed uniformly, 1mL red yeast rice seed is soaked with 30g-40g
Long-grained nonglutinous rice before bubble carries out inoculation proportioning.
4. the production technology of low citrinin Monascus color according to claim 3, it is characterised in that:The red yeast rice culture
Specific method is first to shake long-grained nonglutinous rice culture medium to a pile, heat insulating culture -30 hours 24 hours in 25 DEG C of -35 DEG C of incubators,
Long-grained nonglutinous rice culture medium is shaken up later and is divided out to continue heat insulating culture, daily shaking flask 2 times -3 times, through -7 days 5 days rice grains in purple
Red.
5. the production technology of low citrinin Monascus color according to claim 1, it is characterised in that:The method of the drying
The red yeast rice after hydrogen peroxide carrying out washing treatment to be put into insulating box, under 50 DEG C -70 DEG C of temperature environment, drying is extremely
Moisture is 12% or less.
6. the production technology of the low citrinin Monascus color according to claim 1-5 any one, it is characterised in that:The mistake
The specific method of hydrogen oxide washing is, by the peroxidating through previous process treated red yeast rice is put into concentration 0.8%-1.2%
It is rinsed in hydrogen solution, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:(1.5-3), fully shake up 20 minutes -30 points
Zhong Hou is drained to nothing and is dripped.
7. the production technology of low citrinin Monascus color according to claim 6, it is characterised in that:The method of the irradiation
For will treated that red yeast rice is irradiated using Co 60 through previous process.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810287424.9A CN108477488B (en) | 2018-03-30 | 2018-03-30 | Production process of low citrinin red yeast rice |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810287424.9A CN108477488B (en) | 2018-03-30 | 2018-03-30 | Production process of low citrinin red yeast rice |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108477488A true CN108477488A (en) | 2018-09-04 |
CN108477488B CN108477488B (en) | 2021-08-13 |
Family
ID=63317728
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810287424.9A Active CN108477488B (en) | 2018-03-30 | 2018-03-30 | Production process of low citrinin red yeast rice |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108477488B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116349830A (en) * | 2023-05-05 | 2023-06-30 | 安徽农业大学 | Method for improving production efficiency of red rice by preprocessing long-shaped rice |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101880691A (en) * | 2010-01-14 | 2010-11-10 | 陈豪锋 | Preparation method for brewing functional red yeast rice with low-yield citrinin |
CN102987180A (en) * | 2012-12-13 | 2013-03-27 | 晨光生物科技集团股份有限公司 | Production method for red yeast rice with low citrinin |
CN106360259A (en) * | 2016-08-22 | 2017-02-01 | 张国凤 | Processing method of red rice |
CN106967665A (en) * | 2017-05-24 | 2017-07-21 | 福州大学 | A kind of method that external source adds antioxidant liquid state fermentation monascus low-yield citrinin |
-
2018
- 2018-03-30 CN CN201810287424.9A patent/CN108477488B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101880691A (en) * | 2010-01-14 | 2010-11-10 | 陈豪锋 | Preparation method for brewing functional red yeast rice with low-yield citrinin |
CN102987180A (en) * | 2012-12-13 | 2013-03-27 | 晨光生物科技集团股份有限公司 | Production method for red yeast rice with low citrinin |
CN106360259A (en) * | 2016-08-22 | 2017-02-01 | 张国凤 | Processing method of red rice |
CN106967665A (en) * | 2017-05-24 | 2017-07-21 | 福州大学 | A kind of method that external source adds antioxidant liquid state fermentation monascus low-yield citrinin |
Non-Patent Citations (1)
Title |
---|
李蕙蕙等: "辐照对红曲米中桔霉素及Monacolin K含量的影响 ", 《食品科技》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116349830A (en) * | 2023-05-05 | 2023-06-30 | 安徽农业大学 | Method for improving production efficiency of red rice by preprocessing long-shaped rice |
Also Published As
Publication number | Publication date |
---|---|
CN108477488B (en) | 2021-08-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5756477B2 (en) | Kumestrol production method | |
CN104770300B (en) | A kind of method of fast breeding wool savatier monochasma herb seedling | |
CN104478600B (en) | A kind of preparation method of microbial function microbial inoculum | |
CN106867783A (en) | A kind of brewing process of rice wine | |
CN107446825A (en) | One plant of white fungus bacterial strain and its application | |
CN108165497A (en) | A kind of Monascus Strains breeding method of high yield Mo Nakelin K | |
CN106190889A (en) | A kind of caproic acid fermentation strain and caproic acid extracting method | |
CN103283484B (en) | Method for culturing hericium erinaceus by persimmon sawdust | |
CN106212044A (en) | A kind of Antrodia Camphorata ware formula cultural method | |
CN108477488A (en) | The production technology of low citrinin red yeast rice | |
CN108085343A (en) | The production technology of fermented grain functional Monascus powder | |
CN105230850B (en) | A kind of production method of ganoderma tea | |
CN108486188A (en) | A kind of method of hypha,hyphae fermentation productive fungal polysaccharide | |
CN106967670A (en) | A kind of preparation method of birch-leaf pear protoplast | |
CN109735483A (en) | A kind of improved polyoxin Spore cultivation base and preparation method thereof | |
CN103210791B (en) | Method for cultivating pleurotus citrinopileatus by using persimmon sawdust | |
CN107099421A (en) | Blueberry black rice wine brewage technology | |
CN107267398A (en) | A kind of Liquid Strain of Ganoderma Lucidum culture medium prescription and Liquid Strain of Ganoderma Lucidum cultural method | |
CN104762163B (en) | A kind of method preparing biological song | |
Abbasi et al. | Cichoric acid production from hairy root cultures of Echinacea purpurea grown in a modified airlift bioreactor | |
CN108486164A (en) | The production technology of low citrinin Monascus color | |
CN116287040A (en) | Process for synthesizing epsilon-polylysine by mixing and fermenting streptomycete and mould | |
CN108949846A (en) | A kind of method that high density fermentation improves PQQ yield | |
CN108611381A (en) | It is a kind of to be conducive to the liquid fermentation culturing method that Flavonoid substances are enriched in Phellinus | |
CN107048166A (en) | A kind of method of natural gamma Gamma-propalanine content in increase highland barley monascus |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address |
Address after: 350003 No. 54 North 247 Road, Fujian, Fuzhou Patentee after: Fujian Academy of Agricultural Sciences Agricultural Product Processing Research Institute Country or region after: China Address before: 350003 No. 54, 247 North Road, Gulou District, Fujian, Fuzhou Patentee before: INSTITUTE OF AGRICULTURAL ENGINEERING TECHNOLOGY, FUJIAN ACADEMY OF AGRICULTURAL SCIENCES Country or region before: China |
|
CP03 | Change of name, title or address |