CN108477488A - The production technology of low citrinin red yeast rice - Google Patents

The production technology of low citrinin red yeast rice Download PDF

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CN108477488A
CN108477488A CN201810287424.9A CN201810287424A CN108477488A CN 108477488 A CN108477488 A CN 108477488A CN 201810287424 A CN201810287424 A CN 201810287424A CN 108477488 A CN108477488 A CN 108477488A
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rice
red yeast
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陈慎
杨成龙
黄颖颖
陆东和
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Fujian Academy Of Agricultural Sciences Agricultural Product Processing Research Institute
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Abstract

The present invention relates to a kind of production technologies of low citrinin red yeast rice, and monascus thalline is inoculated in long-grained nonglutinous rice culture medium and carries out grain of rice Spawn incubation to obtain red yeast rice;Then, hydrogen peroxide washing and drying are first carried out, then is irradiated;Alternatively, first being irradiated, then carry out hydrogen peroxide washing and drying;To obtain finished product;The irradiation dose is 7kGy 8kGy.The purpose of the present invention is to provide a kind of production technologies of low citrinin red yeast rice.

Description

The production technology of low citrinin red yeast rice
Technical field
The present invention relates to a kind of production technologies of low citrinin red yeast rice.
Background technology
Citrinin poisons with kidney and with carcinogenicity, however it is the intrinsic secondary metabolite of Monascus.Often Citrinin can be metabolized by advising in the preparation process of red yeast rice, this is the serious obstruction for ensureing red yeast rice safety.
Invention content
The purpose of the present invention is to provide a kind of production technologies of low citrinin red yeast rice.
The purpose of the present invention is achieved through the following technical solutions:A kind of production technology of low citrinin red yeast rice, by red yeast rice Mould body, which is inoculated in long-grained nonglutinous rice culture medium, carries out grain of rice Spawn incubation to obtain red yeast rice;
Then, hydrogen peroxide washing and drying are first carried out, then is irradiated;Alternatively, first being irradiated, then carry out peroxidating Hydrogen is washed and is dried;
To obtain finished product;The irradiation dose is 7kGy-8kGy.
For the prior art, the advantage of the invention is that:Red yeast rice culture is carried out using long-grained nonglutinous rice, and with oxidation and spoke According to combined treatment, technique is relatively simple, efficient, under the premise of ensureing red yeast rice quality (keeping haematochrome to greatest extent), The citrinin of 80%-90% is effectively removed, ensures red yeast rice safety;The highest color value 4500-5500 of finished product red yeast rice;Red yeast rice yield 35%-40%, citrinin content (in terms of unit color value) are less than 0.04mg/U.
Specific implementation mode
The content of present invention is described in detail with reference to embodiment:
Monascus thalline is inoculated with progress grain of rice bacterium in long-grained nonglutinous rice culture medium by a kind of production technology of low citrinin red yeast rice Kind culture is to obtain red yeast rice;
Then, hydrogen peroxide washing and drying are first carried out, then is irradiated;Alternatively, first being irradiated, then carry out peroxidating Hydrogen is washed and is dried;
To obtain finished product;The irradiation dose is 7kGy-8kGy.
The cultural method of the monascus thalline is,
It is prepared by a slant mediums:Brewer's wort 10oB é, are added 2% agar, and pH is adjusted to 6-7, dispenses, goes out after heating for dissolving Bacterium tiltedly puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus (Monascus) spore suspension is inoculated in gnotobasis and is made into through step a Slant medium in, the volume ratio of slant medium and Monascus spore suspension is 1:(0.032-0.06);The red yeast rice The Monascus spore a concentration of 1.5 × 10 of mould spore suspension7A/mL-2.5 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, in 28 DEG C -30 DEG C of temperature Middle culture -11 days 9 days, until inclined-plane is in integrally aubergine.
The specific method of the grain of rice Spawn incubation is,
It is prepared by d long-grained nonglutinous rice culture mediums:500 grams of -600 grams of long-grained nonglutinous rices are put into every 1 liter of water, are impregnated -30 hours 20 hours after stirring evenly, Clear water is carried out later to rinse to no rice milk, then is drained to without dripping, and sterilizing in culture vessel is finally packed into;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through long-grained nonglutinous rice culture medium made from step d in gnotobasis In be sufficiently mixed uniformly;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for that rice grain is in aubergine .
The specific method of the monascus thalline inoculation is, to the inclined-plane culture after monascus thalline culture of every 1mL 1mL-2.5mL sterile waters are added in base and stir evenly, red yeast rice seed is made;Then red yeast rice seed is drawn to be added in long-grained nonglutinous rice culture medium simultaneously It is sufficiently mixed uniformly, the long-grained nonglutinous rice before 1mL red yeast rice seed is impregnated with 30g-40g carries out inoculation proportioning.
The specific method of the red yeast rice culture is first to shake long-grained nonglutinous rice culture medium to one in 25 DEG C of -35 DEG C of incubators Long-grained nonglutinous rice culture medium is shaken up and is divided out to continue heat insulating culture, daily shaking flask 2 by heap, heat insulating culture -30 hours 24 hours later Secondary -3 times, be in aubergine through -7 days 5 days rice grains.
The specific method of hydrogen peroxide washing is, will treated that red yeast rice is put into concentration 0.8%- through previous process It is rinsed in 1.2% hydrogenperoxide steam generator, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:(1.5-3), fully shakes After even -30 minutes 20 minutes, drains to nothing and drip.
The method of the drying is that the red yeast rice after hydrogen peroxide carrying out washing treatment is put into insulating box, in 50 DEG C -70 DEG C temperature environment under, it is dry to moisture be 12% or less.
The method of the irradiation is, will treated that red yeast rice is irradiated using Co 60 through previous process.
It can be obtained finished product after irradiation.
In the present invention, the preferred monascus purpureus of monascus (Monascus purpureus);The method of the sterilizing can To be in 0.08MPa-0.13MPa high pressure steam sterilizations -30 minutes 25 minutes;The gnotobasis can be first 2 hours of inoculation Ultraviolet disinfection is carried out to reach the environment of Sterile standard.
Embodiment 1:
Monascus thalline is inoculated with progress grain of rice bacterium in long-grained nonglutinous rice culture medium by a kind of production technology of low citrinin red yeast rice Then kind culture carries out hydrogen peroxide washing and drying to obtain red yeast rice, finally irradiate and obtain finished product;The irradiation dose is 7kGy。
The cultural method of the monascus thalline is,
It is prepared by a slant mediums:Brewer's wort 10o2% agar is added in B é, and pH is adjusted to 6, is dispensed after heating for dissolving, sterilizing, It tiltedly puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus spore suspension is inoculated with through inclined-plane culture made from step a in gnotobasis In base, the volume ratio of slant medium and Monascus spore suspension is 1:0.06;The red yeast rice of the Monascus spore suspension Mould spore concentration is 1.5 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, is trained in 28 DEG C of temperature It supports 11 days.
The specific method of the grain of rice Spawn incubation is,
It is prepared by d long-grained nonglutinous rice culture mediums:500 grams of long-grained nonglutinous rices are put into every 1 liter of water, is impregnated 20 hours after stirring evenly, carries out clear water later It rinses to no rice milk, then drains to without dripping, be finally packed into sterilizing in culture vessel;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through long-grained nonglutinous rice culture medium made from step d in gnotobasis In be sufficiently mixed uniformly;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for that rice grain is in aubergine .
The specific method of the monascus thalline inoculation is, to the inclined-plane culture after monascus thalline culture of every 1mL 2.5mL sterile waters are added in base and stir evenly, red yeast rice seed is made;Then red yeast rice seed is drawn to be added in long-grained nonglutinous rice culture medium simultaneously fully It is uniformly mixed, the long-grained nonglutinous rice before 1mL red yeast rice seed is impregnated with 30g carries out inoculation proportioning.
The specific method of the red yeast rice culture is first to shake long-grained nonglutinous rice culture medium to a pile in 25 DEG C of incubators, protect Temperature culture 30 hours, long-grained nonglutinous rice culture medium is shaken up and divided out later to continue heat insulating culture 7 days, daily shaking flask 2 times.
The specific method of the hydrogen peroxide washing is that the red yeast rice obtained through grain of rice Spawn incubation is put into concentration It is rinsed in 1.2% hydrogenperoxide steam generator, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:3, fully shake up 20 points Zhong Hou is drained to nothing and is dripped.
The method of the drying is that the red yeast rice after hydrogen peroxide carrying out washing treatment is put into insulating box, in 50 DEG C Under temperature environment, drying to moisture is 12% or less.
The method of the irradiation is, will be oxidation-treated after red yeast rice irradiated using Co 60.
It can be obtained finished product after irradiation.
Embodiment 2:
Monascus thalline is inoculated with progress grain of rice bacterium in long-grained nonglutinous rice culture medium by a kind of production technology of low citrinin red yeast rice Then kind culture carries out hydrogen peroxide washing and drying to obtain red yeast rice, finally irradiate and obtain finished product;The irradiation dose is 7.5kGy。
The cultural method of the monascus thalline is,
It is prepared by a slant mediums:Brewer's wort 10oB é, are added 2% agar, and pH is adjusted to 6.5, dispenses, goes out after heating for dissolving Bacterium tiltedly puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus spore suspension is inoculated with through inclined-plane culture made from step a in gnotobasis In base, the volume ratio of slant medium and Monascus spore suspension is 1:0.05;The red yeast rice of the Monascus spore suspension Mould spore concentration is 2.0 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, is trained in 29 DEG C of temperature It supports 10 days.
The specific method of the grain of rice Spawn incubation is,
It is prepared by d long-grained nonglutinous rice culture mediums:585 grams of long-grained nonglutinous rices are put into every 1 liter of water, is impregnated 24 hours after stirring evenly, carries out clear water later It rinses to no rice milk, then drains to without dripping, be finally packed into sterilizing in culture vessel;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through long-grained nonglutinous rice culture medium made from step d in gnotobasis In be sufficiently mixed uniformly;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for that rice grain is in aubergine .
The specific method of the monascus thalline inoculation is, to the inclined-plane culture after monascus thalline culture of every 1mL 2mL sterile waters are added in base and stir evenly, red yeast rice seed is made;Then red yeast rice seed is drawn to be added in long-grained nonglutinous rice culture medium and fully mix It closes uniformly, the long-grained nonglutinous rice before 1mL red yeast rice seed is impregnated with 36g carries out inoculation proportioning.
The specific method of the red yeast rice culture is first to shake long-grained nonglutinous rice culture medium to a pile in 30 DEG C of incubators, protect Temperature culture 27 hours, long-grained nonglutinous rice culture medium is shaken up and divided out later to continue heat insulating culture 6.5 days, daily shaking flask 3 times.
The specific method of the hydrogen peroxide washing is that the red yeast rice obtained through grain of rice Spawn incubation is put into concentration 1% Hydrogenperoxide steam generator in rinsed, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:2, after fully shaking up 30 minutes, It drains to nothing and drips.
The method of the drying is that the red yeast rice after hydrogen peroxide carrying out washing treatment is put into insulating box, in 60 DEG C Under temperature environment, drying to moisture is 12% or less.
The method of the irradiation is, will be oxidation-treated after red yeast rice irradiated using Co 60.
It can be obtained finished product after irradiation.
Embodiment 3:
Monascus thalline is inoculated with progress grain of rice bacterium in long-grained nonglutinous rice culture medium by a kind of production technology of low citrinin red yeast rice Then kind culture carries out hydrogen peroxide washing and drying to obtain red yeast rice, finally irradiate and obtain finished product;The irradiation dose is 8kGy。
The cultural method of the monascus thalline is,
It is prepared by a slant mediums:Brewer's wort 10o2% agar is added in B é, and pH is adjusted to 7, is dispensed after heating for dissolving, sterilizing, It tiltedly puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus spore suspension is inoculated with through inclined-plane culture made from step a in gnotobasis In base, the volume ratio of slant medium and Monascus spore suspension is 1:0.032;The red yeast rice of the Monascus spore suspension Mould spore concentration is 2.5 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, is trained in 30 DEG C of temperature It supports 9 days.
The specific method of the grain of rice Spawn incubation is,
It is prepared by d long-grained nonglutinous rice culture mediums:600 grams of long-grained nonglutinous rices are put into every 1 liter of water, is impregnated 30 hours after stirring evenly, carries out clear water later It rinses to no rice milk, then drains to without dripping, be finally packed into sterilizing in culture vessel;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through long-grained nonglutinous rice culture medium made from step d in gnotobasis In be sufficiently mixed uniformly;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for that rice grain is in aubergine .
The specific method of the monascus thalline inoculation is, to the inclined-plane culture after monascus thalline culture of every 1mL 1mL sterile waters are added in base and stir evenly, red yeast rice seed is made;Then red yeast rice seed is drawn to be added in long-grained nonglutinous rice culture medium and fully mix It closes uniformly, the long-grained nonglutinous rice before 1mL red yeast rice seed is impregnated with 40g carries out inoculation proportioning.
The specific method of the red yeast rice culture is first to shake long-grained nonglutinous rice culture medium to a pile in 35 DEG C of incubators, protect Temperature culture 24 hours, long-grained nonglutinous rice culture medium is shaken up and divided out later to continue heat insulating culture 5 days, daily shaking flask 3 times.
The specific method of the hydrogen peroxide washing is that the red yeast rice obtained through grain of rice Spawn incubation is put into concentration It is rinsed in 0.8% hydrogenperoxide steam generator, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:1.5, fully shake up 27 After minute, drains to nothing and drip.
The method of the drying is that the red yeast rice after hydrogen peroxide carrying out washing treatment is put into insulating box, in 70 DEG C Under temperature environment, drying to moisture is 12% or less.
The method of the irradiation is, will be oxidation-treated after red yeast rice irradiated using Co 60.
It can be obtained finished product after irradiation.
Embodiment 4:
This example the difference from example 2 is that:In this example, irradiation steps are first carried out, then carry out hydrogen peroxide washing step, Step is finally dried.
Comparative example 1:
This example the difference from example 2 is that:In this example in hydrogen peroxide washing step, be used only etc. weight distilled water It is rinsed;And there is no irradiation steps in this example.
Comparative example 2:
This example the difference from example 2 is that:There is no irradiation steps in this example.
Comparative example 3:
This example the difference from example 2 is that:There is no hydrogen peroxide washing step in this example.
In upper table, the measurement unit of citrinin content (in terms of unit color value) is mg/U.

Claims (7)

1. a kind of production technology of low citrinin red yeast rice, it is characterised in that:Monascus thalline is inoculated in long-grained nonglutinous rice culture medium Grain of rice Spawn incubation is carried out to obtain red yeast rice;
Then, hydrogen peroxide washing and drying are first carried out, then is irradiated;Alternatively, first being irradiated, then carries out hydrogen peroxide and wash It washs and dries;
To obtain finished product;The irradiation dose is 7kGy-8kGy.
2. the production technology of low citrinin Monascus color according to claim 1, it is characterised in that:The monascus thalline Cultural method is,
It is prepared by a slant mediums:10 ° of B é of brewer's wort, are added 2% agar, and pH is adjusted to 6-7, is dispensed after heating for dissolving, sterilizing, tiltedly It puts into after inclined-plane cools down and is made;
B monascuses are inoculated with:Monascus spore suspension is inoculated with through slant medium made from step a in gnotobasis In, the volume ratio of slant medium and Monascus spore suspension is 1:(0.032-0.06);The Monascus spore suspension Monascus spore a concentration of 1.5 × 107A/mL-2.5 × 107A/mL;
C monascus thalline cultures:The slant medium handled through step b is put into incubator, is trained in 28 DEG C -30 DEG C of temperature It supports -11 days 9 days, until inclined-plane is in integrally aubergine.
3. according to the production technology of low citrinin Monascus color described in claim 1, it is characterised in that:The grain of rice Spawn incubation Specific method is,
It is prepared by d long-grained nonglutinous rice culture mediums:500 grams of -600 grams of long-grained nonglutinous rices are put into every 1 liter of water, impregnate -30 hours 20 hours after stirring evenly, later It carries out clear water to rinse to no rice milk, then drains to without dripping, be finally packed into sterilizing in culture vessel;
E monascus thalline are inoculated with:Monascus thalline is inoculated with through being filled in long-grained nonglutinous rice culture medium made from step d in gnotobasis Divide and is uniformly mixed;
F red yeast rice cultures:The long-grained nonglutinous rice culture medium handled through step e is put into incubator and is cultivated, waits for rice grain in aubergine.
The specific method of monascus thalline inoculation is,
1mL-2.5mL sterile waters are added into the slant medium after monascus thalline culture of every 1mL and stir evenly, are made red Sort of quyi;Then it draws red yeast rice seed to be added in long-grained nonglutinous rice culture medium and be sufficiently mixed uniformly, 1mL red yeast rice seed is soaked with 30g-40g Long-grained nonglutinous rice before bubble carries out inoculation proportioning.
4. the production technology of low citrinin Monascus color according to claim 3, it is characterised in that:The red yeast rice culture Specific method is first to shake long-grained nonglutinous rice culture medium to a pile, heat insulating culture -30 hours 24 hours in 25 DEG C of -35 DEG C of incubators, Long-grained nonglutinous rice culture medium is shaken up later and is divided out to continue heat insulating culture, daily shaking flask 2 times -3 times, through -7 days 5 days rice grains in purple Red.
5. the production technology of low citrinin Monascus color according to claim 1, it is characterised in that:The method of the drying The red yeast rice after hydrogen peroxide carrying out washing treatment to be put into insulating box, under 50 DEG C -70 DEG C of temperature environment, drying is extremely Moisture is 12% or less.
6. the production technology of the low citrinin Monascus color according to claim 1-5 any one, it is characterised in that:The mistake The specific method of hydrogen oxide washing is, by the peroxidating through previous process treated red yeast rice is put into concentration 0.8%-1.2% It is rinsed in hydrogen solution, the weight ratio of red yeast rice and hydrogenperoxide steam generator is 1:(1.5-3), fully shake up 20 minutes -30 points Zhong Hou is drained to nothing and is dripped.
7. the production technology of low citrinin Monascus color according to claim 6, it is characterised in that:The method of the irradiation For will treated that red yeast rice is irradiated using Co 60 through previous process.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116349830A (en) * 2023-05-05 2023-06-30 安徽农业大学 Method for improving production efficiency of red rice by preprocessing long-shaped rice

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CN102987180A (en) * 2012-12-13 2013-03-27 晨光生物科技集团股份有限公司 Production method for red yeast rice with low citrinin
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