CN108477224A - Sargassum henslowianum acetic acid ethyl ester extract separation component B and preparation method thereof and the application in preventing and removing marine fouling organisms - Google Patents
Sargassum henslowianum acetic acid ethyl ester extract separation component B and preparation method thereof and the application in preventing and removing marine fouling organisms Download PDFInfo
- Publication number
- CN108477224A CN108477224A CN201810128601.9A CN201810128601A CN108477224A CN 108477224 A CN108477224 A CN 108477224A CN 201810128601 A CN201810128601 A CN 201810128601A CN 108477224 A CN108477224 A CN 108477224A
- Authority
- CN
- China
- Prior art keywords
- acetic acid
- acid ethyl
- ethyl ester
- ester extract
- separation component
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
- C09D5/16—Antifouling paints; Underwater paints
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Materials Engineering (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Application the invention discloses Sargassum henslowianum acetic acid ethyl ester extract separation component B and preparation method thereof and in preventing and removing marine fouling organisms.When the Sargassum henslowianum acetic acid ethyl ester extract separation component B of the present invention is coated on the surface of solids with low dosage, that is, plays the role of that barnacle and Mytilus Edulis Larva is significantly inhibited to adhere to, can be consequently used for preparing preventing and removing marine fouling organisms agent.And Sargassum henslowianum acetic acid ethyl ester extract separation component B is to extract to obtain from Sargassum henslowianum, for naturally occurring organic compound, will not polluted-water environment and by food chain transmission lead to its enrichment in organism, it is environmentally friendly, it is safe, while effectively inhibiting marine organisms attachment, the not heavy metal elements such as cupric and tin, there is good social benefit for environmental protection angle, and it is derived from a wealth of sources, it is easy to detach preparation approach, it is suitable for mass producing, promotion potential is big, it has a good application prospect in preventing and removing marine fouling organisms.
Description
Technical field：
The invention belongs to natural product fields, and in particular to Sargassum henslowianum acetic acid ethyl ester extract separation component B and its
Preparation method and the application in preventing and removing marine fouling organisms.
Background technology：
Marine fouling organism refers to fixation or inhabites in ship and Artificial facilities underwater sites, is generated to economical activities of mankind
The different kind organism of adverse effect, harm predominantly increase resistance, reduce the speed of a ship or plane, increase fuel consumption；Pipe-line system is blocked, is changed
Become Process of Metallic Corrosion, causes corrosion failure；Increase dynamic load(loading) effect, causes artificial facility drift, unbalance or even topple；With
Object contention attachment base and bait are cultivated, its growth and development is interfered, reduces aquatic products quality.Therefore, it is produced in Development of Marine economy
During industry, preventing and removing marine fouling organisms are an indispensable pith always.
Marine fouling organism is by animal, plant and microorganism group at wherein it is main to endanger type that is larger and being difficult to clean off
It is to have calcium carbonate shell, the fixed stockless cirrus class (barnacle) lived of battalion and bivalve (mussel and oyster).In heat
Band, subtropical zone littoral sea, the Typical Representative and the absolute predominance in fouling community that reticulate pattern barnacle is stockless cirrus class
Kind；Perna viridis is then the main bivalve in the East Sea and the South Sea.Fouling organism is generally divided into two life stages,
It is developed to until exploring body surface preparation attachment off and on from larva abjection egg membrane, for the life stage that swims；Larva selects
Good colonization sites are then the fixed or epiphytism stage after the attachment of attachment primary surface, the abnormal formation young.From preventing and kill off angle
From the point of view of, after mankind's generation harm is started from fixation or is adhered to.As can effectively inhibiting larva attachment, it can reach and prevent and kill off mesh
's.Therefore, verification test of the invention uses reticulate pattern barnacle and Perna viridis as experimental subjects, the achievement in research obtained
To have scientific rationality and extensive representativeness.
Conventional marine fouling organism control technology has the defects of of high cost, action time is short, and target organism specific aim is poor,
Environmentally hazardous problem is also even will produce, therefore, understands fully that the antifouling mechanism of action of marine organisms native chemical can be to develop without public affairs
Evil anti-soil technology is offered reference, and marine organisms itself also should be the important sources of novel antifouling agent.Based on anti-attachment activity
Comparision contents of the substance in marine animal, microbial body are few, and structure is relative complex, are not easy to deeply develop and answer extensively
With.So finding natural anti-fouling agent in the sea-plant big from widely distributed, stock number, it is not only a kind of completely new trial, and
And there is important theory and realistic meaning.
Sargassum henslowianum (Sargassum henslowianum C.Agardh) is under the jurisdiction of Phaeophyta, Phaeophyceae, bladder-wrack
Mesh, Sargassaceae, Sargassum are distributed widely on the coastal low tide band rock in the ground such as Fujian, Guangdong and Hong Kong.Its frond is big,
Resourceful, growth cycle is short, can large-scale cultivation, have the good prospect of utilization, recently it has been discovered that it contains
Antitumor, antiviral, reducing blood lipid, ingredient that is anti-oxidant and removing the bioactivity such as free radical.However, being for Sargassum henslowianum
It is no to adhere to the active ingredient with spore germination containing anti-marine fouling organism larva and marime fouling life is applied to
Object prevents and kill off the content in field etc., has no any report at present.
Invention content：
The object of the present invention is to provide Sargassum henslowianum acetic acid ethyl ester extract separation component B and preparation method thereof and in sea
Foreign fouling organism prevent and kill off in application.
The first purpose of the invention is to provide a kind of preparation sides of Sargassum henslowianum acetic acid ethyl ester extract separation component B
Method includes the following steps：
Sargassum henslowianum is extracted with ethanol water, paste extract is obtained after ethanol extract is concentrated；Paste carries
It takes object that petroleum ether and ethyl acetate is used to extract successively, acetic acid ethyl ester extract is obtained after the concentrated drying of acetic acid ethyl acetate extract；
Acetic acid ethyl ester extract is through silica gel column chromatography, using petroleum ether-acetone and chloroform-methanol as eluant, eluent, first from petroleum ether-acetone body
Product ratio 100:0,50:1,20:1,10:1,5:1,3:1,2:1,1:1,1:2, then from chloroform-methanol volume ratio 5:1,3:1,1:1,
0:100 carry out gradient elution successively, collect petroleum ether：Acetone volume ratio 10:The component of 1 elution, then through silica gel column chromatography, with stone
Oily ether-acetone and chloroform-methanol are eluant, eluent, first from petroleum ether-acetone volume ratio 100:1,50:1,20:1,10:1,5:1,3:
1,2:1,1:1,1:2, then from chloroform-methanol volume ratio 5:1,3:1,1:1,0:100 carry out gradient elution, collect petroleum ether：Third
Ketone volume ratio 5:The component of 1 elution, then through LH-20 gel chromatographies twice, respectively with chloroform:Methanol volume ratio 1:1 is eluant, eluent
Elution is collected on lamellae using thin-layer chromatography TLC tracing detections with petroleum ether：Acetone volume ratio 50:11 are used as solvent,
The component that Rf values are 0.60, obtains Sargassum henslowianum acetic acid ethyl ester extract separation component B.
The ethanol water is preferably 95% ethanol water of volume fraction.
Second object of the present invention is to provide a kind of according to above-mentioned Sargassum henslowianum acetic acid ethyl ester extract separation group
The Sargassum henslowianum acetic acid ethyl ester extract separation component B for dividing the preparation method of B to be prepared.
The Sargassum henslowianum acetic acid ethyl ester extract separation component B of the present invention is 10 μ g/cm in coated weight2When, barnacle children
Adhesive rate of the worm after 72 hours is 52.8%, substantially less than the 77.4% of control group, shows that Sargassum henslowianum ethyl acetate extracts
Object separation component B generates apparent inhibiting effect to kentrogon.It is applied in Sargassum henslowianum acetic acid ethyl ester extract separation component B
The amount of covering is 10 μ g/cm2When, adhesive rate of the Mytilus Edulis Larva after 72 hours is only 7.8%, well below control group, shows Heng Shi horses
Tail algae acetic acid ethyl ester extract separation component B can effectively inhibit the attachment of Mytilus Edulis Larva.It can be seen that Sargassum henslowianum acetic acid
Ethyl ester extract separation component B is attached with good inhibiting effect to the larva of stockless cirrus class and bivalve.
Therefore, third object of the present invention is to provide Sargassum henslowianum acetic acid ethyl ester extract separation component B in ocean
Fouling organism prevent and kill off in application.
The marine fouling organism is preferably stockless cirrus class and bivalve.
The stockless cirrus class is preferably reticulate pattern barnacle, and the bivalve is preferably Perna viridis.
It is preferred that the application is that the Sargassum henslowianum acetic acid ethyl ester extract separation component B is coated on solid
Surface, coated weight are 10 μ g/cm2More than.
Fourth object of the present invention is to provide Sargassum henslowianum acetic acid ethyl ester extract separation component B and is preparing ocean dirt
Damage the application in biological insect control agent.
The preventing and removing marine fouling organisms agent is preferably kentrogon agent for preventing and eliminating or Mytilus Edulis Larva agent for preventing and eliminating.
Compared with prior art, the present invention has the advantages that：
When the Sargassum henslowianum acetic acid ethyl ester extract separation component B low dosages of the present invention are coated on the surface of solids, that is, have
The apparent effect for inhibiting marine organisms attachment, can be consequently used for preparing preventing and removing marine fouling organisms agent.And Heng Shi horse hairs
Algae acetic acid ethyl ester extract separation component B is naturally occurring organic compound, polluted-water environment and will not pass through food chain
Transmission leads to its enrichment in organism, environmentally friendly, safe, while effectively inhibiting marine organisms attachment,
The not heavy metal elements such as cupric and tin have good social benefit for environmental protection angle, and Sargassum henslowianum comes
Source is very extensive, and Sargassum henslowianum acetic acid ethyl ester extract separation component B separation preparation approachs are easy, are suitable for extensive life
Production, promotion potential is big, has a good application prospect in preventing and removing marine fouling organisms.
Specific implementation mode：
The following examples are further illustrations of the invention, rather than limiting the invention.
Embodiment 1：
Sargassum henslowianum acetic acid ethyl ester extract separation component B is to extract to obtain from Sargassum henslowianum, separation and Extraction
Step includes：
(1) by Sargassum henslowianum (Sargassum henslowianum C.Agardh) 95% ethanol water of volume fraction
Solution extracts 3 times, merges extracting solution and obtains ethanol extract, 45 DEG C of rotary evaporation in vacuo concentrations remove ethyl alcohol, obtain paste and carry
Take object；
(2) paste extract is added water with 6 milliliters every gram of ratio and is resuspended, and first extracts 3 respectively with isometric petroleum ether
It is secondary, petroleum ether extraction component is discarded, remaining water layer is extracted 3 times with isometric ethyl acetate, the concentration of combined ethyl acetate extract liquor
Acetic acid ethyl ester extract is obtained after drying；
(3) acetic acid ethyl ester extract is carried out in normal pressure silica gel column (silica gel 8000g, column length 180cm, column diameter 20cm)
Chromatography, using petroleum ether-acetone and chloroform-methanol as eluant, eluent, first from petroleum ether-acetone volume ratio 100:0,50:1,20:
1,10:1,5:1,3:1,2:1,1:1,1:2, then from chloroform-methanol volume ratio 5:1,3:1,1:1,0:100 carry out gradient successively washes
It is de-, tracing detection is carried out using thin-layer chromatography TLC, obtains 25 components after merging similar compositions, and it is Fr.1-25 to number；
(4) by step (3) petrochina ether：Acetone volume ratio 10:The combined component Fr.2 of 1 elution is in normal pressure silica gel column (silicon
Glue 250g, column length 50cm, column diameter 4cm) in carry out silica gel column chromatography separation, be elution with petroleum ether-acetone and chloroform-methanol
Agent, first from petroleum ether-acetone volume ratio 100:0,50:1,20:1,10:1,5:1,3:1,2:1,1:1,1:2, then from chloroform-first
Alcohol volume ratio 5:1,3:1,1:1,0:100 carry out gradient elution successively, carry out tracing detection using thin-layer chromatography TLC, merge phase
Like component.Wherein petroleum ether：Acetone volume ratio 10:The combined component of 1 elution, again passes by silicagel column (silica gel 250g, column length
50cm, column diameter 4cm) chromatography, using petroleum ether-acetone and chloroform-methanol as eluant, eluent, first from petroleum ether-acetone volume
Than 100:1,50:1,20:1,10:1,5:1,3:1,2:1,1:1,1:2, then from chloroform-methanol volume ratio 5:1,3:1,1:1,0:
100 carry out gradient elution, carry out tracing detection using thin-layer chromatography TLC, merge similar compositions.Wherein petroleum ether：Acetone volume
Than 5:The combined component Fr.2-1 of 1 elution is detached by Sephadex LH-20 gel chromatographies, with chloroform:Methanol volume ratio 1:1
For eluent, the collection of eluting fraction is carried out according to the speed of 8min/ pipes, and is compiled according to the time sequencing of collection
Number, number 1-60, using thin-layer chromatography TLC tracing detections, wherein 25-35 pipes are similar compositions (with petroleum ether：Acetone body
Product ratio 50:11 are used as solvent, obtain 2 spots, and Rf values are respectively 0.47 and 0.60), merge after 25-35 pipe components using
Sephadex LH-20 gel chromatographies detach, with chloroform:Methanol volume ratio 1:1 is eluent, according to the speed of 6min/ pipes
The collection of eluting fraction is carried out, and is numbered according to the time sequencing of collection, number 1 ' -45 ', using thin-layer chromatography TLC
Tracing detection, wherein 30 ' -33 ' manage as same component (with petroleum ether：Acetone volume ratio 50:11 are used as solvent, and Rf values are
0.60) it is Sargassum henslowianum acetic acid ethyl ester extract separation component B, to merge after concentrating.
Embodiment 2：
Experimental group：By Sargassum henslowianum acetic acid ethyl ester extract separation component B chloroform methanol mixed solvent (chloroform methanols
Mixed solvent is by chloroform and methanol by volume 3:1 mixes) dissolving, compound concentration is the solution of 282.6 μ g/ml.
The 1ml solution is added in the culture dish of a diameter of 6cm, and makes its uniform fold culture dish bottom.After solvent completely volatilization, apply
The dosage for being overlying on the Sargassum henslowianum acetic acid ethyl ester extract separation component B of culture dish bottom is 10 μ g/cm2.Then it is cultivating
13ml seawater is added in ware.
Control group：Adding 1ml chloroform methanols mixed solvent, (chloroform methanol mixed solvent is by chloroform and methanol by volume 3:
1 mixes), so that it is uniformly distributed in culture dish bottom, it is to be evaporated to add 13ml seawater completely.
Blank group：13ml seawater is added.
Experimental group, blank group and control group respectively set 4 Duplicate Samples.30 reticulate pattern barnacle Venus are separately added into each sample
Larva.It is placed in the constant incubator that temperature is 30 DEG C or so and is cultivated in dark surrounds.It is primary every observation in 24 hours.Culture
The attachment final to each group larva and death state are for statistical analysis after 72 hours.
Table 1 lists experimental group, the attachment of control group and blank group cyprids and the death rate.As it can be seen that in constant temperature incubation
After being cultivated 72 hours in case, the larva adhesive rate of blank group is 76.3%, control group 77.4%, blank group and control group Venus children
The adhesive rate of worm is without significant difference (P>0.05) influence cyprids work is will not leave after, showing the volatilization of chloroform methanol mixed solvent
The harmful substance of property.As for the experimental group of culture dish bottom covering Sargassum henslowianum acetic acid ethyl ester extract separation component B, gold
The adhesive rate of star larva is 52.8%, is less than control group, and significant difference (P<0.05), show that Sargassum henslowianum ethyl acetate extracts
Take object separation component B in 10 μ g/cm2Dosage under can effectively inhibit the attachments of reticulate pattern barnacle cyprids.In addition, blank
The larval mortality of group, control group and experimental group is 0, shows Sargassum henslowianum acetic acid ethyl ester extract separation component B at this
Dosage will not generate toxic action to larva.
Table 1：Attachment and death state of the reticulate pattern barnacle cyprids after 72 hours
| Group | Proof load | Adhesive rate (%) | The death rate (%) |
| Blank group | 76.3 | 0 | |
| Control group | 77.4 | 0 | |
| Experimental group | 10μg/cm2 | 52.8 | 0 |
Embodiment 3：
Experimental group：By Sargassum henslowianum acetic acid ethyl ester extract separation component B chloroform methanol mixed solvent (chloroform methanols
Mixed solvent is by chloroform and methanol by volume 3:1 mixes) dissolving, compound concentration is the solution of 282.6 μ g/ml.
The 1ml solution is added in the culture dish of a diameter of 6cm and makes its uniform fold culture dish bottom.After solvent completely volatilization, apply
The Sargassum henslowianum acetic acid ethyl ester extract separation component B dosage for being overlying on culture dish bottom is 10 μ g/cm2.Then in culture dish
Middle addition 13ml seawater.
Control group：Adding 1ml chloroform methanols mixed solvent, (chloroform methanol mixed solvent is by chloroform and methanol by volume 3:
1 mixes), so that solvent is uniformly distributed in culture dish bottom, waits for that solvent volatilization is complete, add 13ml seawater.
Blank group：13ml seawater is added.
Experimental group, blank group and control group are all provided with 4 Duplicate Samples, and about 30 Perna viridis faces are added in each sample
Larva.It is cultivated under dark surrounds in the incubator that temperature is about 26 DEG C.It is primary every observation in 24 hours.After culture 72 hours
The attachment final to each group larva and death state are for statistical analysis.
Table 2 lists experimental group, the attachment of control group and blank group Perna viridis larva and the death rate.As it can be seen that in constant temperature
After being cultivated 72 hours in incubator, the larva adhesive rate of blank group is about 47.2%, control group about 44.6%, blank group and control
The adhesive rate of group veliger is without significant difference (P>0.05) influence face is will not leave after, showing the volatilization of chloroform methanol mixed solvent
The active harmful substance of disk larva.And through the experimental group of Sargassum henslowianum acetic acid ethyl ester extract separation component B processing, larva
Adhesive rate be only 7.8%, well below control group, difference extremely significantly (P<0.01), show Sargassum henslowianum ethyl acetate
Extract separation component B can effectively inhibit the attachment of Perna viridis larva.In addition, the children of blank group, control group and experimental group
The worm death rate is 0, shows that Sargassum henslowianum acetic acid ethyl ester extract separation component B will not generate poisoning in the dosage to larva
Effect.
Table 2：Attachment and death state of the Perna viridis veliger after 72 hours
| Group | Proof load | Adhesive rate % | Death rate % |
| Blank group | 47.2 | 0 | |
| Control group | 44.6 | 0 | |
| Experimental group | 10μg/cm2 | 7.8 | 0 |
Claims (9)
1. a kind of preparation method of Sargassum henslowianum acetic acid ethyl ester extract separation component B, which is characterized in that including following step
Suddenly：Sargassum henslowianum is extracted with ethanol water, paste extract is obtained after ethanol extract is concentrated；Paste extract according to
It is secondary to be extracted with petroleum ether and ethyl acetate, obtain acetic acid ethyl ester extract after the concentrated drying of acetic acid ethyl acetate extract；Acetic acid second
Ester extract is through silica gel column chromatography, using petroleum ether-acetone and chloroform-methanol as eluant, eluent, first from petroleum ether-acetone volume ratio
100:0,50:1,20:1,10:1,5:1,3:1,2:1,1:1,1:2, then from chloroform-methanol volume ratio 5:1,3:1,1:1,0:100
Gradient elution is carried out successively, collects petroleum ether：Acetone volume ratio 10:The component of 1 elution, then through silica gel column chromatography, with petroleum ether-
Acetone and chloroform-methanol are eluant, eluent, first from petroleum ether-acetone volume ratio 100:1,50:1,20:1,10:1,5:1,3:1,2:
1,1:1,1:2, then from chloroform-methanol volume ratio 5:1,3:1,1:1,0:100 carry out gradient elution, collect petroleum ether：Acetone body
Product ratio 5:The component of 1 elution, then through LH-20 gel filtration chromatographies twice, respectively with chloroform:Methanol volume ratio 1:1 washes for eluant, eluent
It is de-, using thin-layer chromatography TLC tracing detections, collect on lamellae with petroleum ether：Acetone volume ratio 50:11 are used as solvent, Rf
Value is 0.60 component, obtains Sargassum henslowianum acetic acid ethyl ester extract separation component B.
2. preparation method according to claim 1, which is characterized in that the ethanol water is 95% second of volume fraction
Alcohol solution.
3. a kind of preparation method system according to Sargassum henslowianum acetic acid ethyl ester extract separation component B as claimed in claim 1 or 2
Standby obtained Sargassum henslowianum acetic acid ethyl ester extract separation component B.
4. the Sargassum henslowianum acetic acid ethyl ester extract separation component B described in claim 3 is in preventing and removing marine fouling organisms
Using.
5. application according to claim 4, which is characterized in that the marine fouling organism is stockless cirrus class and bivalve
Class mollusk.
6. application according to claim 5, which is characterized in that the stockless cirrus class is reticulate pattern barnacle, and described is double
Shell class mollusk is Perna viridis.
7. according to the application described in claim 4,5 or 6, which is characterized in that the application is by the Sargassum henslowianum
Acetic acid ethyl ester extract separation component B is coated on the surface of solids, and coated weight is 10 μ g/cm2More than.
8. the Sargassum henslowianum acetic acid ethyl ester extract separation component B described in claim 3 is preparing preventing and removing marine fouling organisms
Application in agent.
9. application according to claim 8, which is characterized in that the preventing and removing marine fouling organisms agent is anti-for kentrogon
Except agent or Mytilus Edulis Larva agent for preventing and eliminating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810128601.9A CN108477224B (en) | 2018-02-08 | 2018-02-08 | Component B separated from sargassum henryi ethyl acetate extract, preparation method thereof and application of component B in marine fouling organism prevention and removal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810128601.9A CN108477224B (en) | 2018-02-08 | 2018-02-08 | Component B separated from sargassum henryi ethyl acetate extract, preparation method thereof and application of component B in marine fouling organism prevention and removal |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108477224A true CN108477224A (en) | 2018-09-04 |
| CN108477224B CN108477224B (en) | 2020-12-08 |
Family
ID=63339961
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810128601.9A Expired - Fee Related CN108477224B (en) | 2018-02-08 | 2018-02-08 | Component B separated from sargassum henryi ethyl acetate extract, preparation method thereof and application of component B in marine fouling organism prevention and removal |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108477224B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5629045A (en) * | 1992-09-17 | 1997-05-13 | Richard L. Veech | Biodegradable nosiogenic agents for control of non-vertebrate pests |
| US20040235901A1 (en) * | 2003-02-20 | 2004-11-25 | Kem William Reade | Materials and methods for inhibiting fouling of surfaces exposed to aquatic environments |
| JP2006160665A (en) * | 2004-12-07 | 2006-06-22 | Terra Bio Remedic Co Ltd | Marine organism adhesion-preventing agent and its use |
| EP2198713A1 (en) * | 2007-09-07 | 2010-06-23 | National University Corporation Hokkaido University | Extract of red alga laurencia sp. with organic solvent, and agent for prevention of the settlement of barnacle comprising compound isolated from the extract |
| CN102757673A (en) * | 2011-04-27 | 2012-10-31 | 香港科技大学 | Application of amide compound in prevention of marine biological pollution |
| CN104592798A (en) * | 2014-12-29 | 2015-05-06 | 岭南师范学院 | Application of aurantiamide acetate in preventing and removing marine fouling organisms |
-
2018
- 2018-02-08 CN CN201810128601.9A patent/CN108477224B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5629045A (en) * | 1992-09-17 | 1997-05-13 | Richard L. Veech | Biodegradable nosiogenic agents for control of non-vertebrate pests |
| US20040235901A1 (en) * | 2003-02-20 | 2004-11-25 | Kem William Reade | Materials and methods for inhibiting fouling of surfaces exposed to aquatic environments |
| JP2006160665A (en) * | 2004-12-07 | 2006-06-22 | Terra Bio Remedic Co Ltd | Marine organism adhesion-preventing agent and its use |
| EP2198713A1 (en) * | 2007-09-07 | 2010-06-23 | National University Corporation Hokkaido University | Extract of red alga laurencia sp. with organic solvent, and agent for prevention of the settlement of barnacle comprising compound isolated from the extract |
| CN102757673A (en) * | 2011-04-27 | 2012-10-31 | 香港科技大学 | Application of amide compound in prevention of marine biological pollution |
| CN104592798A (en) * | 2014-12-29 | 2015-05-06 | 岭南师范学院 | Application of aurantiamide acetate in preventing and removing marine fouling organisms |
Non-Patent Citations (1)
| Title |
|---|
| 吴慧: "亨氏马尾藻醇提取物化学成分的分离鉴定及生物活性的初步筛选", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108477224B (en) | 2020-12-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101715770B (en) | Application of loop proline-3-hydroxy-2-aminobutyric acid in preventing and removing marine fouling organisms | |
| Hessami et al. | Evaluation of tropical seaweeds as feedstock for bioethanol production | |
| CN101536687A (en) | Use of flavone and isoflavone derivative as anti-biofouling substances and method and paint for preventing marine biofouling | |
| CN102919285B (en) | Method for extracting effective algae inhibition refinement component from barley | |
| Dar et al. | Effect of seasonal variation on the anti-inflammatory activity of Sargassum wightii growing on the N. Arabian Sea coast of Pakistan | |
| CN104592798B (en) | Application of the acetic acid orange acid amides in preventing and removing marine fouling organisms | |
| Saeidnia et al. | Biological activity of two red algae, Gracilaria salicornia and Hypnea flagelliformis from Persian Gulf. | |
| CN101724305B (en) | Application of cycle (proline-alanine) in preventing and removing marine fouling organism | |
| CN108056113A (en) | Sargassum henslowianum acetic acid ethyl ester extract separation component E and preparation method thereof and the application in preventing and removing marine fouling organisms | |
| CN102617529B (en) | Application for isobenzofuran type compounds in marine biofouling prevention and preparation method thereof | |
| CN108477224A (en) | Sargassum henslowianum acetic acid ethyl ester extract separation component B and preparation method thereof and the application in preventing and removing marine fouling organisms | |
| CN108308199A (en) | Sargassum henslowianum acetic acid ethyl ester extract separation component D and preparation method thereof and the application in preventing and removing marine fouling organisms | |
| CN108124908A (en) | Sargassum henslowianum acetic acid ethyl ester extract separation component C and preparation method thereof and the application in preventing and removing marine fouling organisms | |
| CN108056112A (en) | Sargassum henslowianum acetic acid ethyl ester extract separation component A and preparation method thereof and the application in preventing and removing marine fouling organisms | |
| CN102613201B (en) | Isoflavanone compound for protecting underwater structure surface and application thereof | |
| KR100386417B1 (en) | Antifouling agent extracted from marine algae | |
| CN108484628B (en) | Application of quassin lactone compounds in preventing marine biofouling | |
| CN110724149B (en) | Indole alkaloid dimer compound derived from marine fungi, preparation method and application of indole alkaloid dimer compound in marine fouling organism resistant control agent | |
| CN102321089A (en) | Screw ring alkaloid compound, preparation method thereof and application in the aspect of marine organism fouling resistance | |
| CN111406759B (en) | Grateloupia filicina extract and application thereof in preparation of marine fouling organism control agent | |
| CN101717594B (en) | Application of loop proline-leucine in preventing and removing marine fouling organisms | |
| CN105394057B (en) | Application of the epiberberine in preventing and removing marine fouling organisms agent is prepared | |
| Khalid et al. | Studies on the bioactivity and phycochemistry of Lyngbya Agardh (Cyanophycota) | |
| CN105461514B (en) | A sponge diterpene compound, a preparing method thereof and applications of the diterpene compound | |
| CN105325432B (en) | Application of the jamaicin in preventing and removing marine fouling organisms agent is prepared |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201208 Termination date: 20220208 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |