CN108469686A - A kind of optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens - Google Patents
A kind of optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens Download PDFInfo
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- CN108469686A CN108469686A CN201810658731.3A CN201810658731A CN108469686A CN 108469686 A CN108469686 A CN 108469686A CN 201810658731 A CN201810658731 A CN 201810658731A CN 108469686 A CN108469686 A CN 108469686A
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- 239000004005 microsphere Substances 0.000 title claims abstract description 60
- 230000003287 optical effect Effects 0.000 title claims abstract description 44
- 238000003384 imaging method Methods 0.000 title claims abstract description 41
- 238000012576 optical tweezer Methods 0.000 title claims abstract description 37
- 238000006073 displacement reaction Methods 0.000 claims abstract description 8
- 230000005540 biological transmission Effects 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 230000003321 amplification Effects 0.000 claims description 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 59
- 101000857682 Homo sapiens Runt-related transcription factor 2 Proteins 0.000 description 10
- 102100025368 Runt-related transcription factor 2 Human genes 0.000 description 10
- 238000010586 diagram Methods 0.000 description 6
- 230000000007 visual effect Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 230000004438 eyesight Effects 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
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- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
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Abstract
The optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens that the present invention provides a kind of, for biomedical and material science research field.A kind of optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens, including CCD, laser, semi-transparent semi-reflecting lens, object lens, microsphere lens, sample cell, sample and mobile platform, sample is placed in sample cell, and microsphere lens is independently controlled by light near sample surface；Sample cell is placed on mobile platform；CCD is used to receive the light of the first semi-transparent semi-reflecting lens reflection, and shows super-resolution image.The present invention has broken the optical diffraction limit of light microscope using microsphere lens, and optical tweezer and microsphere lens microtechnic are blended, and can carry out real time imagery to nano level sample.For target to be observed, the position that microballoon and sample distance make focussing plane lock target to be observed can be adjusted first with the optical optical tweezers system that laser and object lens are constituted, displacement platform is recycled to carry out super resolution dynamic observation and scanning imagery.
Description
Technical field
The optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens that the present invention relates to a kind of specifically utilizing laser
The distance between microsphere lens and sample are controlled with the optical optical tweezers system that object lens are constituted, using the movement of three-D displacement platform to sample
Carry out optical ultra-discrimination rate scanning imagery, it may be implemented to the sample of nanostructure into super-resolution imaging, can be used for biology
The research fields such as medicine and material science.
Background technology
Limited by diffraction limit, the resolution ratio of conventional optical microscope can only achieve the half of lambda1-wavelength, and one
A little its complex manufacturing technology of super-resolution microscope have strict requirements to imaging.Such as scanning electron microscope and transmission electron microscope are to sample
It requires, and in vacuo sample need to be imaged.It is not suitable for observing living body biological sample, and scanning near-field optical is micro-
Mirror then requires optical fiber probe to be sufficiently close to be imaged with sample surfaces, and the time being imaged is longer, and observation cannot in real time
It is implemented.The present invention controls microballoon using the stress effect of light, realizes the imaging of oversubscription variability.By a diameter of micron or milli
The microballoon of rice is controlled in sample surfaces, can significantly improve the resolution capability of conventional optical microscope.
Invention content
The technical problem to be solved by the present invention is to：There is provided it is a kind of may be implemented optical ultra-discrimination rate imaging based on optical tweezer
With the optical ultra-discrimination rate imaging system of microsphere lens.
Technical scheme of the present invention：
A kind of optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens, including it is CCD1, laser 2, semi-transparent semi-reflecting
Mirror 3, object lens 4, microsphere lens 5, sample 6, sample cell 7 and mobile platform 8, sample 6 are placed in sample cell 7,5 quilt of microsphere lens
Optical tweezer controls near 6 surface of sample；Sample cell 7 is placed on mobile platform 8；CCD1 is anti-for receiving the first semi-transparent semi-reflecting lens 3
The light penetrated, and show super-resolution image.
Sample 6 is fixed in sample cell 7, and change microballoon by adjusting the optical optical tweezers system that laser 2 and object lens 4 are constituted
The distance between lens 5 and sample 6；So that laser 2 is transmitted by semi-transparent semi-reflecting lens 3, then by object lens 4, eventually passes through microsphere lens 5
It focuses in the plane to be observed of sample 6, observation area is arrived by 8 mobile example 6 of displacement platform, the light that sample 6 reflects is through micro-
Globe lens 5, then reflected through the first semi-transparent semi-reflecting lens 3 by object lens 4 and finally received by CCD1 and super-resolution image is presented.
The photocontrol is by the way of：Laser 2 first penetrates the first semi-transparent semi-reflecting lens 3, using object lens 4, directly controls
Microsphere lens 5 processed.
The optical ultra-discrimination rate imaging system further includes galvanometer 9, galvanometer 9 be located at semi-transparent semi-reflecting lens 3 and object lens 4 it
Between.
First semi-transparent semi-reflecting lens of optical ultra-discrimination rate imaging system 3 are replaced by dichroscope 10, and in CCD1 and
Increase by the second semi-transparent semi-reflecting lens 12 between first semi-transparent semi-reflecting lens 3, light source 11 is transmitted through the second semi-transparent semi-reflecting lens 12.
First semi-transparent semi-reflecting lens 3 and laser transmission direction angle at 45 °.
Second semi-transparent semi-reflecting lens 12 and light source transmission direction angle at 45 °.
The a diameter of micron or millimeter of the microsphere lens 5.
The amplification factor of the object lens 4 is not less than 40 times.
Beneficial effects of the present invention：
The present invention has broken the optical diffraction limit of light microscope using microsphere lens, and optical tweezer and microsphere lens is micro-
Technology blends, and can carry out real time imagery to nano level sample.
For target to be observed, microballoon 5 and sample 6 can be adjusted first with the optical optical tweezers system that laser 2 and object lens 4 are constituted
Distance makes focussing plane lock the position of target to be observed, recycles displacement platform to carry out super resolution dynamic and observes and be scanned into
Picture.
When carrying out real time imagery to sample, without additionally modifying sample.
Description of the drawings
Fig. 1 (a) be a kind of the first form of the optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens just
Set microscope schematic diagram.
Fig. 1 (b) is a kind of falling for the first form of the optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens
Set microscope schematic diagram.
Fig. 2 (a) be a kind of second of form of the optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens just
Set microscope schematic diagram.
Fig. 2 (b) is a kind of falling for second of form of the optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens
Set microscope schematic diagram.
Fig. 3 (a) be a kind of the third form of the optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens just
Set microscope schematic diagram.
Fig. 3 (b) is a kind of falling for the third form of the optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens
Set microscope schematic diagram.
In figure：1 CCD；2 laser；3 first semi-transparent semi-reflecting lens；4 object lens；5 microsphere lens；6 samples；7 sample cells；
8 mobile platforms；9 galvanometers；10 dichroscopes；11 light sources；12 second semi-transparent semi-reflecting lens.
Specific implementation mode
Below in conjunction with the accompanying drawings, the specific implementation mode of the system of the present invention is described.
In dynamic observation imaging, the relative position of microsphere lens 5 and object lens 4 will immobilize；Used microsphere lens 2
A diameter of micron or millimeter, and microballoon is immersed in the liquid of sample cell.
As shown in a, b of Fig. 1, in a specific embodiment, a kind of optical ultra-discrimination rate based on optical tweezer and microsphere lens
Imaging system, including CCD1, light source 2, semi-transparent semi-reflecting lens 3, object lens 4, microsphere lens 5, sample 6, sample cell 7 and mobile platform
8, sample of the microsphere lens 5 by laser 2 through the first semi-transparent semi-reflecting lens 3 using the photocontrol of object lens 4 in sample cell 7
Right over product 6 or underface, sample 6 are fixed in sample cell 7, a diameter of micron or millimeter of microsphere lens and are immersed in sample
In the liquid in pond, first semi-transparent semi-reflecting lens 3 are in 45 degree between laser and object lens and with light, and the CCD1 is used
In the light that through first semi-transparent semi-reflecting lens 3 reflects of the reception with sample message.
It is for the dynamic imaging step in specific implementation method：Adjustment mobile platform 8 makes sample 6 in the visual field of object lens 4
It is interior, then adjust laser 2 closely makes field-of-view image adjust at a distance from the optical optical tweezers system control microsphere lens and sample that object lens 4 are constituted
To most clearly then position is scanned imaging using displacement platform 8 to sample.And by these dynamic super-resolution visions
Image is sent in computer.
As shown in a, b of Fig. 2, in a specific embodiment, a kind of optical ultra-discrimination rate based on optical tweezer and microsphere lens
Imaging system, including it is CCD1, laser 2, the first semi-transparent semi-reflecting lens 3, object lens 4, microsphere lens 5, sample 6, sample cell 7, mobile flat
Platform 8 and galvanometer 9.The microsphere lens 5 is reflected by galvanometer 9 eventually by object lens 4 by laser 2 through semi-transparent semi-reflecting lens 3 again
Photocontrol is right over sample 6 in sample cell 7 or underface, sample 6 are fixed in sample cell 7, the diameter of microsphere lens
For micron or millimeter and be immersed in the liquid of sample cell, the semi-transparent semi-reflecting lens 3 between laser 2 and galvanometer 9 and with
Light is in 45 degree, and the CCD1 is used to receive the light reflected through semi-transparent semi-reflecting lens 3 with sample message.
Dynamic imaging step for specific implementation method is：Adjustment mobile platform 8 makes sample 6 in the visual field of object lens 4,
Adjust laser 2 again closely makes field-of-view image be adjusted at a distance from the optical optical tweezers system control microsphere lens and sample that object lens 4 are constituted
Most clearly position, is then scanned imaging using displacement platform 8 or galvanometer 9 to sample.And by these dynamic super-resolutions
Visual pattern is sent in computer.
As shown in a, b of Fig. 3, in a specific embodiment, a kind of optical ultra-discrimination rate based on optical tweezer and microsphere lens
Imaging system, including CCD1, laser 2, dichroscope 10, object lens 4, microsphere lens 5, sample 6, sample cell 7, mobile platform 8, light
Source 11 and the second semi-transparent semi-reflecting lens 12.The microsphere lens 5 is by laser 2 through dichroscope 10 using the light-operated of object lens 4
It makes right over the sample 6 in sample cell 7 or underface, the light of light source passes through the reflection of the second semi-transparent semi-reflecting lens 12 using two
Reflection to Look mirror 10 is beaten eventually by object lens 4 on sample 6, and second semi-transparent semi-reflecting lens 12 and light are in 45 degree, institute
The sample 6 stated is fixed in sample cell 7, a diameter of micron or millimeter of microsphere lens 5 and is immersed in the liquid of sample cell, institute
The dichroscope 10 stated is in 45 degree between laser 2 and object lens 4 and with light, and the CCD1 believes for receiving with sample
The light of breath transmitted through the second semi-transparent semi-reflecting lens 12.
Dynamic imaging step for specific implementation method is：Adjustment mobile platform 8 makes sample 6 in the visual field of object lens 4,
Adjust laser 2 again closely makes field-of-view image be adjusted at a distance from the optical optical tweezers system control microsphere lens and sample that object lens 4 are constituted
Most clearly then position is scanned imaging using displacement platform 8 to sample.And it is these dynamic super-resolution visions are color
Color image is sent in computer.
In conclusion under the premise of not departing from the scope and spirit described in the claims in the present invention, also there are many improve
Should all include in scope of the presently claimed invention with variation.
Claims (9)
1. a kind of optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens, which is characterized in that described based on optical tweezer
With the optical ultra-discrimination rate imaging system of microsphere lens, including CCD (1), laser (2), the first semi-transparent semi-reflecting lens (3), object lens
(4), microsphere lens (5), sample (6), sample cell (7) and mobile platform (8), sample (6) are placed in sample cell (7), microballoon
Lens (5) are independently controlled by light near sample (6) surface；Sample cell (7) is placed on mobile platform (8)；CCD (1) is for receiving the
The light of one semi-transparent semi-reflecting lens (3) reflection, and show super-resolution image；
The optical tweezer system that sample (6) is fixed in the liquid of sample cell (7), and is constituted by adjusting laser (2) and object lens (4)
System changes the distance between microsphere lens (5) and sample (6)；So that laser (2) is transmitted by the first semi-transparent semi-reflecting lens (3), then passes through
Object lens (4) eventually pass through microsphere lens (5) and focus in the plane to be observed of sample (6), and sample is moved by displacement platform (8)
Product (6) arrive observation area, and the light of sample (6) reflection is through microsphere lens (5), then by object lens (4) through the first semi-transparent semi-reflecting lens (3)
Reflection is finally received by CCD (1) and super-resolution image is presented；
The photocontrol is by the way of：Laser (2) first penetrates the first semi-transparent semi-reflecting lens (3), using object lens (4), directly
Control microsphere lens (5).
2. the optical ultra-discrimination rate imaging system according to claim 1 based on optical tweezer and microsphere lens, which is characterized in that
The optical ultra-discrimination rate imaging system further includes galvanometer (9), galvanometer (9) be located at semi-transparent semi-reflecting lens (3) and object lens (4) it
Between.
3. the optical ultra-discrimination rate imaging system according to claim 1 based on optical tweezer and microsphere lens, which is characterized in that
The first semi-transparent semi-reflecting lens (3) are replaced by dichroscope (10) in the optical ultra-discrimination rate imaging system, and in CCD (1) and
Increase the second semi-transparent semi-reflecting lens (12) between semi-transparent semi-reflecting lens (3), light source (11) is transmitted through the second semi-transparent semi-reflecting lens (12).
4. the optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens according to claim 1-3, feature exist
In, first semi-transparent semi-reflecting lens (3) and laser (2) transmission direction angle at 45 °, second semi-transparent semi-reflecting lens (12)
With light source (11) transmission direction angle at 45 °.
5. special according to any optical ultra-discrimination rate imaging systems based on optical tweezer and microsphere lens of claim 1-3
Sign is, a diameter of micron or millimeter of the microsphere lens (5).
6. the optical ultra-discrimination rate imaging system according to claim 4 based on optical tweezer and microsphere lens, which is characterized in that
The a diameter of micron or millimeter of the microsphere lens (5).
7. according to any optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens in claim 1,2,3 or 6,
It is characterized in that, the amplification factor of the object lens (4) is not less than 40 times.
8. the optical ultra-discrimination rate imaging system according to claim 4 based on optical tweezer and microsphere lens, which is characterized in that
The amplification factor of the object lens (4) is not less than 40 times.
9. the optical ultra-discrimination rate imaging system according to claim 5 based on optical tweezer and microsphere lens, which is characterized in that
The amplification factor of the object lens (4) is not less than 40 times.
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| CN201810658731.3A CN108469686A (en) | 2018-06-20 | 2018-06-20 | A kind of optical ultra-discrimination rate imaging system based on optical tweezer and microsphere lens |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109269980A (en) * | 2018-10-16 | 2019-01-25 | 中国科学院光电技术研究所 | High-resolution optical detection method based on single optical tweezers medium microspheres |
| CN109387481A (en) * | 2018-11-28 | 2019-02-26 | 同济大学 | A kind of apparatus and method detecting circular dichroism |
| CN110082282A (en) * | 2019-04-18 | 2019-08-02 | 天津大学 | The method and apparatus for realizing optical ultra-discrimination imaging based on optical tweezer |
| CN110132920A (en) * | 2019-05-16 | 2019-08-16 | 北京大学 | A kind of optical ultra-discrimination imaging devices and methods therefor based on laser manipulation microballoon mirror |
| CN111381355A (en) * | 2018-12-29 | 2020-07-07 | 南京培轩雅谱光电科技有限公司 | Optical imaging apparatus and method |
| CN112461830A (en) * | 2020-11-05 | 2021-03-09 | 山东建筑大学 | Combined transparent medium microsphere small-sized optical tweezers device and application |
| CN113484322A (en) * | 2021-07-13 | 2021-10-08 | 天津大学 | Optical tweezers super-resolution imaging method and system capable of feeding back axial optical trap position in real time |
| CN113608287A (en) * | 2021-08-06 | 2021-11-05 | 暨南大学 | Micro lens and application thereof |
| CN114077168A (en) * | 2022-01-06 | 2022-02-22 | 之江实验室 | Super-resolution laser direct writing and real-time imaging device and method based on optical tweezers microspheres |
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| CN109269980A (en) * | 2018-10-16 | 2019-01-25 | 中国科学院光电技术研究所 | High-resolution optical detection method based on single optical tweezers medium microspheres |
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| CN110132920A (en) * | 2019-05-16 | 2019-08-16 | 北京大学 | A kind of optical ultra-discrimination imaging devices and methods therefor based on laser manipulation microballoon mirror |
| CN112461830A (en) * | 2020-11-05 | 2021-03-09 | 山东建筑大学 | Combined transparent medium microsphere small-sized optical tweezers device and application |
| CN113484322A (en) * | 2021-07-13 | 2021-10-08 | 天津大学 | Optical tweezers super-resolution imaging method and system capable of feeding back axial optical trap position in real time |
| CN113484322B (en) * | 2021-07-13 | 2023-01-10 | 天津大学 | Optical tweezers super-resolution imaging method and system capable of feeding back axial optical trap position in real time |
| CN113608287A (en) * | 2021-08-06 | 2021-11-05 | 暨南大学 | Micro lens and application thereof |
| CN114077168A (en) * | 2022-01-06 | 2022-02-22 | 之江实验室 | Super-resolution laser direct writing and real-time imaging device and method based on optical tweezers microspheres |
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