CN108456024A - A kind of repairing method of microorganism of organic castoff compost containing heavy metal - Google Patents
A kind of repairing method of microorganism of organic castoff compost containing heavy metal Download PDFInfo
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- CN108456024A CN108456024A CN201810066775.7A CN201810066775A CN108456024A CN 108456024 A CN108456024 A CN 108456024A CN 201810066775 A CN201810066775 A CN 201810066775A CN 108456024 A CN108456024 A CN 108456024A
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- Prior art keywords
- microorganism
- heavy metal
- organic
- repairing method
- filler
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/80—Separation, elimination or disposal of harmful substances during the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
A kind of repairing method of microorganism of organic castoff compost containing heavy metal, is related to a kind of organic castoff compost repairing method of microorganism.The problem of bringing secondary pollution the present invention is to solve existing repairing method of microorganism.Method:One, the organic waste containing heavy metal is chosen;Two, after taking filler to be soaked in organic carbon source, taking-up is dried spare;Three, the mechanism difference of heavy metal pollution is handled for specified microorganisms to determine the time of addition filler;Four, after composting cycle is for 2 weeks, spherical porous medium class filler is taken out for regular turning in composting process;Five, step 3 is repeated to step 4, until content of beary metal reaches desired value in material.The organic carbon source nutrient solution that this method is carried by filler extracts the microorganism that heavy metal has been carried in soil, to make heavy metal contaminants thoroughly eradicate.The present invention is used for organic castoff compost field.
Description
Technical field
The present invention relates to a kind of organic castoff compost repairing method of microorganism.
Background technology
In recent years, with the rapid development of China's livestock and poultry breeding industry, a large amount of feces of livestock and poultry is produced.And China livestock and poultry are raised
The phenomenon that adding micro heavy there are excess in material, some minor metallic elements such as copper, zinc, arsenic act not only as animal
Growth necessary to nutrient, and can play disease preventing and treating, improve feed efficiency, promote growth of animal the effects that, therefore also by
It is widely used in feed addictive.Since heavy metal cannot fully absorb metabolism, most of shape with active compound in animal body
Formula is excreted with excrement, these hazardous contaminants not only seriously threaten the quality safety of China's animal products, Er Qiesui
It animal manures application and enters soil, water body, huge potential hazard is brought to environmental and human health impacts.Heavy metal is in environment
In existing forms include:1) it is dissolved in water;2) it is present in the ion-exchange sites of surface particles;3) it adsorbs or is complexed solid
Body particle surface;4) exist in a manner of co-precipitation;5) it is combined to form complex with organic matter;6) it is present in the lattice of minerals
In.As it can be seen that heavy metal can exist in a variety of forms in the environment, the heavy metal bonding mechanism and toxicity of different shape are not
Together.Aerobic compost and anaerobic digestion are that feces of livestock and poultry is innoxious, main means of recycling, and compost is a complicated object
Reason, chemistry and bioprocess, the mutual mechanism of action of composting material internal mix object is more complicated, so as to cause compost shadow
The factor of sound is more.Root is it was found that controllable process technology includes mainly:Moisture, oxygen supply, temperature, C/N ratios, additive, by reasonable
Ground regulates and controls to reduce the biologically effective form of heavy metal, the heavy metal being passivated in composting material.But when external environment is (such as temperature
Degree, pH etc.) change when, the form of heavy metal can also may change correspondingly again, cause secondary pollution, cannot practically solve weight
Rnetal contamination problem.
There are many heavy metals removal methods at present, as chemical deposition, physical-chemical process, phytoremediation, microorganism are repaiied
Multiple method, wherein microorganism remediation technology have at low cost, repair time is short, operation is easy, bacterial strain can by tame raising patience,
Many advantages, such as strain resource is abundant, low energy consumption is the ideal method of the following solution heavy metal pollution problem.But by
It still will cannot therefrom be extracted for the microorganism of reparation in current technology, wait for microorganism after death vertical distribution
It can be discharged, can not be removed heavy metal is practical, the problem of secondary pollution can be brought again again.So greatly limiting micro-
Biological prosthetic application and popularization.
Invention content
The problem of bringing secondary pollution the present invention is to solve existing repairing method of microorganism, provide a heavy metal species
Organic castoff compost repairing method of microorganism.
Heavy metal organic castoff compost repairing method of microorganism of the present invention, carries out according to the following steps:
One, the organic waste containing heavy metal is chosen, adjusting moisture to 60%-70% (quality), C/N is (19-21):1;
Two, after taking the anti-spherical porous medium class filler of degradation to be soaked in organic carbon source, drying for standby is taken out;
Three, the mechanism difference of heavy metal pollution is handled for specified microorganisms to determine the time of addition filler, specifically such as
Under:
Remediation microbial inoculum, is first inoculated at step 2 by the microorganism remediation microbial inoculum that huge sum of money genotype is adsorbed for extracellular polymeric
In filler after reason, uniformly puddles in the organic waste after step 1 adjusting and carry out compost;
The microorganism remediation microbial inoculum of huge sum of money genotype is absorbed for intracellular, after remediation microbial inoculum is first inoculated into step 1 adjusting
Compost is carried out in organic waste, in the phase in the microorganism growth index end of term, after step 2 processing is uniformly blended into compost material
Filler;
Four, regular turning in composting process, water tariff collection is 55%~65%, and temperature control is at 45 DEG C hereinafter, compost is all
After phase is for 2 weeks, spherical porous medium class filler is taken out, measures content of beary metal on filler;
Five, step 3 is repeated to step 4, until content of beary metal reaches desired value in material.
Further, organic waste is feces of livestock and poultry, sludge, house refuse or agricultural wastes (such as straw in step 1
Stalk) etc..
Further, dry for drying or air-dried described in step 2.
Further, filler described in step 2 is spherical porous medium, such as cotton ball, sponge, polyamide, have compared with
Big porosity, porosity reach 50% or more.
Further, organic carbon source described in step 2 is the solution containing abundant carbon source such as glucose, and phosphorus content reaches
20% or more.
Further, the filler after being impregnated described in step 2 will be dried or be air-dried, it is made to keep dry and containing free
Gap makes it keep drying, nutrition and habitat can be provided for microorganism, and be in state that is half-full or having gap.
Further, the microorganism remediation microbial inoculum of the absorption of extracellular polymeric described in step 3 huge sum of money genotype is not understand sugar vacation
Anthropi (Pseudochrobactrum asaccharolyticum) ZS2, bacillus licheniformis (Bacillus
Licheniformis) R08 or Nostoc (Nostoc);The wherein described puzzled sugar vacation anthropi ZS2 is in article《It is anti-
The separation identification and anti-metal characteristic research of zinc bacterium ZS2》It is disclosed in (Qu Jia, biotechnology notification, 2016.08), lichens bud
Spore bacillus R08 is in article《Bacillus licheniformis R08 adsorbs Ag+Physicochemical characteristics research》It is public in (Sun Daohua, 2008)
It opens, the Nostoc is in article《Polyethylene Glycol Simulated drought stress gives birth to liquid suspension culture hair-like Nostoc
Long and Physiology and biochemistry influence》(fourth is shaken, 2012).
Further, the microorganism remediation microbial inoculum that intracellular described in step 3 absorbs huge sum of money genotype is acinetobacter calcoaceticus
(Acinetobacter sp.) USTB-F or resistance to metals covet copper bacterium (Cupriavidus metallidurans) CH34.Wherein institute
Acinetobacter calcoaceticus USTB-F is stated in article《The enriched character of the one plant of acinetobacter calcoaceticus of resistance to copper USTB-F》(Environmental Chemistry, in April, 2011
The 4th phase of volume 30) in disclose, the resistance to metal covet copper bacterium CH34 in article《Greedy copper bacterium 6-5 Two-Component Signaling Systems czcR2-
The clone of czcS2 and bioinformatic analysis》It is disclosed in (Chinese agronomy notification, 2014).
Further, regular turning described in step 4 uniformly repairs compost material mainly for changing filler position.
Further, temperature described in step 4 should be controlled at 45 DEG C hereinafter, being in order to avoid microbe inoculation is by height
Temperature injury.
Beneficial effects of the present invention:
1, the organic carbon source nutrient solution that this method is carried by filler will carry the microorganism of heavy metal in soil
It extracts, to make heavy metal contaminants thoroughly eradicate.The removal rate that filler heavy metal is intended for single use in this method is more than 30%,
The removal rate of filler heavy metal is repeatedly replaced up to 60% or more.
2, since the method for the present invention is will to carry the spherical porous medium class filler taking-up of microorganism, to effectively will
Heavy metal is extracted from compost, avoids secondary pollution.
3, the method for the present invention is economic and practical, easy to operate, extraction effect is good, repair time is short, energy conservation and environmental protection, and there is no weights
Metal secondary pollution problem.
Specific implementation mode
Technical solution of the present invention is not limited to act specific implementation mode set forth below, further includes between each specific implementation mode
Arbitrary combination.
Specific implementation mode one:Present embodiment heavy metal organic castoff compost repairing method of microorganism, by following step
It is rapid to carry out:
One, the organic waste containing heavy metal is chosen, adjusting moisture to 60%-70% (quality), C/N is (19-21):1;
Two, after taking the anti-spherical porous medium class filler of degradation to be soaked in organic carbon source, drying for standby is taken out;
Three, the mechanism difference of heavy metal pollution is handled for specified microorganisms to determine the time of addition filler, specifically such as
Under:
Remediation microbial inoculum, is first inoculated at step 2 by the microorganism remediation microbial inoculum that huge sum of money genotype is adsorbed for extracellular polymeric
In filler after reason, uniformly puddles in the organic waste after step 1 adjusting and carry out compost;
The microorganism remediation microbial inoculum of huge sum of money genotype is absorbed for intracellular, after remediation microbial inoculum is first inoculated into step 1 adjusting
Compost is carried out in organic waste, in the phase in the microorganism growth index end of term, after step 2 processing is uniformly blended into compost material
Filler;
Four, regular turning in composting process, water tariff collection is 55%~65%, and temperature control is at 45 DEG C hereinafter, compost is all
After phase is for 2 weeks, spherical porous medium class filler is taken out;
Five, step 3 is repeated to step 4, until content of beary metal reaches desired value in material.
Specific implementation mode two:The present embodiment is different from the first embodiment in that:Organic waste in step 1
For feces of livestock and poultry, sludge, house refuse or agricultural wastes.It is other same as the specific embodiment one.
Specific implementation mode three:The present embodiment is different from the first and the second embodiment in that:It is dry described in step 2
To dry or air-drying.It is other the same as one or two specific embodiments.
Specific implementation mode four:Unlike one of present embodiment and specific implementation mode one to three:Institute in step 2
It is spherical porous medium, specially cotton ball, sponge or polyamide to state filler.Other phases one of with specific implementation mode one to three
Together.
Specific implementation mode five:Present embodiment is unlike specific implementation mode four:The spherical porous medium
Porosity is 50% or more.It is other identical as specific implementation mode four.
Specific implementation mode six:Unlike one of present embodiment and specific implementation mode one to five:Institute in step 2
It is glucose solution to state organic carbon source.It is other identical as one of specific implementation mode one to five.
Specific implementation mode seven:Unlike one of present embodiment and specific implementation mode one to six:Institute in step 3
The microorganism remediation microbial inoculum for stating extracellular polymeric absorption huge sum of money genotype is not understand the false anthropi (Pseudochrobactrum of sugar
Asaccharolyticum) ZS2, bacillus licheniformis (Bacillus licheniformis) R08 or Nostoc
(Nostoc).It is other identical as one of specific implementation mode one to six.
Specific implementation mode eight:Unlike one of present embodiment and specific implementation mode one to seven:Institute in step 3
It is acinetobacter calcoaceticus (Acinetobacter sp.) USTB-F or resistance to metals to state intracellular to absorb the microorganism remediation microbial inoculum of huge sum of money genotype
Greedy copper bacterium (Cupriavidus metallidurans) CH34.It is other identical as one of specific implementation mode one to seven.
Specific implementation mode nine:Unlike one of present embodiment and specific implementation mode one to eight:Water in step 4
Divide and is maintained at 60%.It is other identical as one of specific implementation mode one to eight.
Elaborate below to the embodiment of the present invention, following embodiment under based on the technical solution of the present invention into
Row is implemented, and gives detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following realities
Apply example.
Experimental example 1:
The present embodiment heavy metal organic castoff compost is directed to the repairing method of microorganism of extracellular absorbent-type, including following step
Suddenly:
Once, take 1kg to add chicken and the cow dung of feed growth, and measure Zn content in excrement, adjust moisture to 60%-
70%, C/N is about 20:1;
Two, cotton ball is taken to be soaked in the glucose solution of mass concentration 0.5% after immersion 12h, taking-up is dried spare;
Three, puzzled sugar vacation anthropi (Pseudochrobactrum asaccharolyticum) ZS2 is inoculated in step
In rapid two treated cotton balls, uniformly puddles in the excrement after step 1 adjusting and carry out compost;
Four, regular turning in composting process, water tariff collection is 60% or so, and temperature control is at 40 DEG C or so, composting cycle
After for 2 weeks, cotton ball is taken out, measures content of beary metal on filler;
Five, step 3 is repeated to step 4, until content of beary metal reaches desired value in material.
Control group:Remediation microbial inoculum is not inoculated in step 2 with differing only in for the present embodiment method.
It is multiple to repeat above procedure, finally measures after compost Zn-ef ficiency content in sample and filler (cotton ball), and convert
It is as shown in table 1 at quality.
Table 1
This experimental example proves that the method for the present invention can quickly remove the heavy metal in compost, can be removed in compost in 60 days
Heavy metal.Single experiment heavy metal removing rate is more than 30%, replaces 10 fillers and repeats experiment removal rate up to 60% or more.
The method of the present invention is detached and is removed from truly realizing heavy metal from compost sample, and does not generate secondary pollution.
Experimental example 2:
The present embodiment heavy metal organic castoff compost is directed to the microorganism adsorption method of intracellular absorption-type, including following step
Suddenly:
Once, take 1kg to add the cow dung of feed growth, and measure wherein copper content, it is big to 60%-70%, C/N to adjust moisture
About 20:1;
Two, cotton ball is taken to be soaked in the glucose solution of mass concentration 0.5% after immersion 12h, taking-up is dried spare;
Three, acinetobacter calcoaceticus (Acinetobacter sp.) USTB-F is inoculated into the cow dung of step 1 and carries out compost, often
Every the OD values that 30 minutes measure microorganism, in the phase in the microorganism growth index end of term (after OD values reach peak value), in compost material
It is uniformly blended into step 2 treated cotton ball;
Four, regular turning in compost, 60% or so, temperature is controlled at 40 DEG C or so, by cotton ball after 10 water tariff collection
It takes out, measures content of beary metal on filler;
Five, step 3 is repeated to step 4, until heavy metal amount reaches desired value in material.
Control group:Remediation microbial inoculum is not inoculated in step 2 with differing only in for the present embodiment method.
It is multiple to repeat above procedure, finally measures after compost copper coin cellulose content in sample and cotton, and be converted into quality such as
Shown in table 2.
Table 2
Original compost sample | Experimental group compost sample | Control group compost sample | Experimental group filler | Control group filler | |
Cow dung | 51.02mg | 29.67mg | 49.48mg | 19.94mg | 1.03mg |
This experimental example goes removing heavy metals, single experiment heavy metal removing rate to be more than 15% for intracellular absorption-type microbial inoculum, more
The removal rate for changing 10 filler heavy metals reaches 40% or more.Illustrate that the method for the present invention is feasible and practical, it can be from real meaning
Removing heavy metals up reduce environmental hazard, and do not generate secondary pollution.
Claims (8)
1. a kind of repairing method of microorganism of organic castoff compost containing heavy metal, it is characterised in that this method according to the following steps into
Row:
One, the organic waste containing heavy metal is chosen, adjusting moisture to 60%-70%, C/N is (19-21):1;
Two, after taking the anti-spherical porous medium class filler of degradation to be soaked in organic carbon source, drying for standby is taken out;
Three, the time of addition filler is determined for the mechanism difference of specified microorganisms processing heavy metal pollution, it is specific as follows:
The microorganism remediation microbial inoculum of huge sum of money genotype is adsorbed for extracellular polymeric, after remediation microbial inoculum is first inoculated in step 2 processing
Filler in, uniformly puddle in step 1 adjusting after organic waste in carry out compost;
The microorganism remediation microbial inoculum of huge sum of money genotype is absorbed for intracellular, is first inoculated into remediation microbial inoculum organic after step 1 is adjusted
Compost is carried out in waste, in the phase in the microorganism growth index end of term, is uniformly blended into step 2 in compost material treated and fill out
Material;
Four, regular turning in composting process, water tariff collection is 55%~65%, and temperature control is at 45 DEG C hereinafter, composting cycle is held
After 2 weeks continuous, spherical porous medium class filler is taken out;
Five, step 3 is repeated to step 4, until content of beary metal reaches desired value in material.
2. one kind repairing method of microorganism of organic castoff compost containing heavy metal according to claim 1, it is characterised in that:
Organic waste is feces of livestock and poultry, sludge, house refuse or agricultural wastes in step 1.
3. one kind repairing method of microorganism of organic castoff compost containing heavy metal according to claim 1 or 2, feature exist
In:It is dry for drying or air-dried described in step 2.
4. one kind repairing method of microorganism of organic castoff compost containing heavy metal according to claim 3, it is characterised in that:
Filler described in step 2 is spherical porous medium, specially cotton ball, sponge or polyamide.
5. one kind repairing method of microorganism of organic castoff compost containing heavy metal according to claim 4, it is characterised in that:
The porosity of the spherical porous medium is 50% or more.
6. one kind repairing method of microorganism of organic castoff compost containing heavy metal according to claim 1,2 or 4, feature
It is:Organic carbon source described in step 2 is glucose solution.
7. one kind repairing method of microorganism of organic castoff compost containing heavy metal according to claim 6, it is characterised in that:
The microorganism remediation microbial inoculum that extracellular polymeric described in step 3 adsorbs huge sum of money genotype is not understand the false anthropi of sugar
(Pseudochrobactrum asaccharolyticum) ZS2, bacillus licheniformis (Bacillus licheniformis)
R08 or Nostoc (Nostoc).
8. one kind repairing method of microorganism of organic castoff compost containing heavy metal according to claim 7, it is characterised in that:
The microorganism remediation microbial inoculum that intracellular described in step 3 absorbs huge sum of money genotype is acinetobacter calcoaceticus (Acinetobacter sp.)
USTB-F or resistance to metals covet copper bacterium (Cupriavidus metallidurans) CH34.
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