CN108445130B - Method for finely screening optimal characteristic peptide segments in milk powder allergic protein - Google Patents
Method for finely screening optimal characteristic peptide segments in milk powder allergic protein Download PDFInfo
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- CN108445130B CN108445130B CN201810219686.1A CN201810219686A CN108445130B CN 108445130 B CN108445130 B CN 108445130B CN 201810219686 A CN201810219686 A CN 201810219686A CN 108445130 B CN108445130 B CN 108445130B
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- enzymolysis
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- peptide fragments
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 36
- 238000012216 screening Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 28
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 27
- 235000013336 milk Nutrition 0.000 title claims abstract description 20
- 239000008267 milk Substances 0.000 title claims abstract description 20
- 210000004080 milk Anatomy 0.000 title claims abstract description 20
- 230000000172 allergic effect Effects 0.000 title claims abstract description 17
- 208000010668 atopic eczema Diseases 0.000 title claims abstract description 17
- 239000000843 powder Substances 0.000 title claims abstract description 17
- 239000000243 solution Substances 0.000 claims abstract description 80
- 102000007079 Peptide Fragments Human genes 0.000 claims abstract description 67
- 108010033276 Peptide Fragments Proteins 0.000 claims abstract description 67
- 239000012488 sample solution Substances 0.000 claims abstract description 32
- 238000001819 mass spectrum Methods 0.000 claims abstract description 25
- 239000012086 standard solution Substances 0.000 claims abstract description 15
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000001514 detection method Methods 0.000 claims abstract description 8
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims abstract description 8
- NCGWKCHAJOUDHQ-UHFFFAOYSA-N n,n-diethylethanamine;formic acid Chemical compound OC=O.OC=O.CCN(CC)CC NCGWKCHAJOUDHQ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001195 ultra high performance liquid chromatography Methods 0.000 claims abstract description 6
- 238000004445 quantitative analysis Methods 0.000 claims abstract description 5
- PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000007853 buffer solution Substances 0.000 claims abstract description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 35
- 239000004202 carbamide Substances 0.000 claims description 35
- 238000006243 chemical reaction Methods 0.000 claims description 14
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 10
- 239000000523 sample Substances 0.000 claims description 9
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 235000019419 proteases Nutrition 0.000 claims description 8
- 238000001228 spectrum Methods 0.000 claims description 6
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 5
- XBJFCYDKBDVADW-UHFFFAOYSA-N acetonitrile;formic acid Chemical compound CC#N.OC=O XBJFCYDKBDVADW-UHFFFAOYSA-N 0.000 claims description 5
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 5
- 235000019253 formic acid Nutrition 0.000 claims description 5
- HQVFCQRVQFYGRJ-UHFFFAOYSA-N formic acid;hydrate Chemical compound O.OC=O HQVFCQRVQFYGRJ-UHFFFAOYSA-N 0.000 claims description 5
- 229910052708 sodium Inorganic materials 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 238000002372 labelling Methods 0.000 claims description 4
- 238000004704 ultra performance liquid chromatography Methods 0.000 claims description 4
- 102000004142 Trypsin Human genes 0.000 claims description 3
- 108090000631 Trypsin Proteins 0.000 claims description 3
- 239000013566 allergen Substances 0.000 claims description 3
- 230000002009 allergenic effect Effects 0.000 claims description 3
- 238000010844 nanoflow liquid chromatography Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000012588 trypsin Substances 0.000 claims description 3
- 108010067770 Endopeptidase K Proteins 0.000 claims description 2
- 108010051815 Glutamyl endopeptidase Proteins 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 4
- 235000018102 proteins Nutrition 0.000 description 18
- 229920001184 polypeptide Polymers 0.000 description 9
- 102000004196 processed proteins & peptides Human genes 0.000 description 9
- 235000020248 camel milk Nutrition 0.000 description 4
- 235000020250 donkey milk Nutrition 0.000 description 4
- 235000020251 goat milk Nutrition 0.000 description 4
- 235000016046 other dairy product Nutrition 0.000 description 4
- 108010076119 Caseins Proteins 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 102000007562 Serum Albumin Human genes 0.000 description 2
- 108010071390 Serum Albumin Proteins 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000037029 cross reaction Effects 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102100038920 Alpha-S1-casein Human genes 0.000 description 1
- 108050000244 Alpha-s1 casein Proteins 0.000 description 1
- 108050001786 Alpha-s2 casein Proteins 0.000 description 1
- 102000012045 Casein, beta Human genes 0.000 description 1
- 102000011632 Caseins Human genes 0.000 description 1
- 102000004407 Lactalbumin Human genes 0.000 description 1
- 108090000942 Lactalbumin Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- -1 beta-lactalbumin Proteins 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013568 food allergen Substances 0.000 description 1
- 238000004896 high resolution mass spectrometry Methods 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 235000021241 α-lactalbumin Nutrition 0.000 description 1
- 235000021247 β-casein Nutrition 0.000 description 1
- 235000021246 κ-casein Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/067—Preparation by reaction, e.g. derivatising the sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201810219686.1A CN108445130B (en) | 2018-03-16 | 2018-03-16 | Method for finely screening optimal characteristic peptide segments in milk powder allergic protein |
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CN201810219686.1A CN108445130B (en) | 2018-03-16 | 2018-03-16 | Method for finely screening optimal characteristic peptide segments in milk powder allergic protein |
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CN108445130A CN108445130A (en) | 2018-08-24 |
CN108445130B true CN108445130B (en) | 2019-12-31 |
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CN201810219686.1A Active CN108445130B (en) | 2018-03-16 | 2018-03-16 | Method for finely screening optimal characteristic peptide segments in milk powder allergic protein |
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Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103995077A (en) * | 2014-05-21 | 2014-08-20 | 中国计量科学研究院 | Method for determining content of beta-lactoglobulin in milk powder |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5242697A (en) * | 1990-10-26 | 1993-09-07 | Maurizio Luca | Method of preparing nutrified food products |
CN1082339A (en) * | 1992-08-17 | 1994-02-23 | 王景和 | Protein particle fodder for milk cow and preparation method thereof |
CN103134881A (en) * | 2013-02-01 | 2013-06-05 | 浙江省疾病预防控制中心 | Cattle 2, 2-bipyridine-lactalbumin quantitative detection reagent box and application thereof |
CN106980021B (en) * | 2017-03-31 | 2018-10-26 | 李森康 | A kind of protein detection techniques based on enzymolysis polypeptide principle being able to verify that hydrolysis result |
-
2018
- 2018-03-16 CN CN201810219686.1A patent/CN108445130B/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103995077A (en) * | 2014-05-21 | 2014-08-20 | 中国计量科学研究院 | Method for determining content of beta-lactoglobulin in milk powder |
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CN108445130A (en) | 2018-08-24 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Method for finely screening optimal feature peptide fragment in milk powder allergic protein Effective date of registration: 20200528 Granted publication date: 20191231 Pledgee: China Merchants Bank Co.,Ltd. Hangzhou Branch Pledgor: GREENTOWN NONGKE DETECTION TECHNOLOGY Co.,Ltd. Registration number: Y2020330000281 |
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Date of cancellation: 20210526 Granted publication date: 20191231 Pledgee: China Merchants Bank Co.,Ltd. Hangzhou Branch Pledgor: GREENTOWN NONGKE DETECTION TECHNOLOGY Co.,Ltd. Registration number: Y2020330000281 |
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Denomination of invention: A method for fine screening of the optimal characteristic peptide in milk powder allergenic protein Effective date of registration: 20210527 Granted publication date: 20191231 Pledgee: China Merchants Bank Co.,Ltd. Hangzhou Branch Pledgor: GREENTOWN NONGKE DETECTION TECHNOLOGY Co.,Ltd. Registration number: Y2021330000474 |
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Date of cancellation: 20220523 Granted publication date: 20191231 Pledgee: China Merchants Bank Co.,Ltd. Hangzhou Branch Pledgor: GREENTOWN NONGKE DETECTION TECHNOLOGY Co.,Ltd. Registration number: Y2021330000474 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for fine screening the best characteristic peptide in milk powder allergy protein Effective date of registration: 20220614 Granted publication date: 20191231 Pledgee: China Merchants Bank Co.,Ltd. Hangzhou Branch Pledgor: GREENTOWN NONGKE DETECTION TECHNOLOGY Co.,Ltd. Registration number: Y2022330000949 |
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Date of cancellation: 20230815 Granted publication date: 20191231 Pledgee: China Merchants Bank Co.,Ltd. Hangzhou Branch Pledgor: GREENTOWN NONGKE DETECTION TECHNOLOGY Co.,Ltd. Registration number: Y2022330000949 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A Method for Fine Screening the Optimal Characteristic Peptide Segments in Milk Powder Allergic Proteins Effective date of registration: 20230822 Granted publication date: 20191231 Pledgee: China Merchants Bank Co.,Ltd. Hangzhou Branch Pledgor: GREENTOWN NONGKE DETECTION TECHNOLOGY Co.,Ltd. Registration number: Y2023980053183 |
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