CN108444978A - It is a kind of based on dendritic gold nano body structure surface enhance Raman spectrum ferroheme detection method and its application - Google Patents
It is a kind of based on dendritic gold nano body structure surface enhance Raman spectrum ferroheme detection method and its application Download PDFInfo
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- CN108444978A CN108444978A CN201810587697.5A CN201810587697A CN108444978A CN 108444978 A CN108444978 A CN 108444978A CN 201810587697 A CN201810587697 A CN 201810587697A CN 108444978 A CN108444978 A CN 108444978A
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 86
- 239000010931 gold Substances 0.000 title claims abstract description 85
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 85
- 238000001514 detection method Methods 0.000 title claims abstract description 44
- 238000001237 Raman spectrum Methods 0.000 title claims abstract description 30
- 239000002086 nanomaterial Substances 0.000 claims abstract description 38
- 238000000034 method Methods 0.000 claims abstract description 31
- 238000001069 Raman spectroscopy Methods 0.000 claims abstract description 26
- 230000002708 enhancing effect Effects 0.000 claims abstract description 14
- 230000000694 effects Effects 0.000 claims abstract description 11
- 102000001554 Hemoglobins Human genes 0.000 claims abstract description 8
- 108010054147 Hemoglobins Proteins 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 34
- 239000000243 solution Substances 0.000 claims description 32
- 239000007788 liquid Substances 0.000 claims description 14
- 239000002105 nanoparticle Substances 0.000 claims description 14
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000013019 agitation Methods 0.000 claims description 9
- 239000006185 dispersion Substances 0.000 claims description 9
- 239000013049 sediment Substances 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 7
- 210000004369 blood Anatomy 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 238000012417 linear regression Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- BMUDPLZKKRQECS-UHFFFAOYSA-K 3-[18-(2-carboxyethyl)-8,13-bis(ethenyl)-3,7,12,17-tetramethylporphyrin-21,24-diid-2-yl]propanoic acid iron(3+) hydroxide Chemical compound [OH-].[Fe+3].[N-]1C2=C(C)C(CCC(O)=O)=C1C=C([N-]1)C(CCC(O)=O)=C(C)C1=CC(C(C)=C1C=C)=NC1=CC(C(C)=C1C=C)=NC1=C2 BMUDPLZKKRQECS-UHFFFAOYSA-K 0.000 claims description 3
- 230000005284 excitation Effects 0.000 claims description 3
- 229940109738 hematin Drugs 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 238000004445 quantitative analysis Methods 0.000 claims description 3
- 210000003743 erythrocyte Anatomy 0.000 claims description 2
- 230000010354 integration Effects 0.000 claims description 2
- 239000012452 mother liquor Substances 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims 1
- 238000010998 test method Methods 0.000 claims 1
- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 abstract description 5
- 238000005259 measurement Methods 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract description 2
- 235000019441 ethanol Nutrition 0.000 description 9
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
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- 230000001404 mediated effect Effects 0.000 description 2
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- 239000000758 substrate Substances 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SJUCACGNNJFHLB-UHFFFAOYSA-N O=C1N[ClH](=O)NC2=C1NC(=O)N2 Chemical compound O=C1N[ClH](=O)NC2=C1NC(=O)N2 SJUCACGNNJFHLB-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229940124344 antianaemic agent Drugs 0.000 description 1
- 239000003173 antianemic agent Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002082 metal nanoparticle Substances 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 150000003233 pyrroles Chemical class 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of detection method of ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface and its application, this method includes that (1) prepares dendritic gold nano structure-solution；(2) haemachrome solution is prepared；(3) standard sample is prepared；(4) standard curve of dendritic gold nano structure detection hemoglobin concentration is established；(5) concentration of ferroheme in sample to be tested is detected.Surface-enhanced Raman effect the present invention is based on dendritic gold nano structure to ferroheme realizes ferroheme by surface enhanced Raman spectroscopy analysis method and efficiently, quickly detects by dendritic gold nano structure first Application in the measurement of ferroheme.This method is easy to operate, and detection sensitivity is high, and detection limit is low, and stability is good.
Description
Technical field
The invention belongs to technical field of biological, and in particular to a kind of detection method of ferroheme, more particularly to one
Kind enhances detection method and its application of the ferroheme of Raman spectrum based on dendritic gold nano body structure surface.
Background technology
Ferroheme is important native metal metalloporphyrin complex, is the activity of hemoglobin and myoglobins etc. in organism
Center, and as a kind of natural pigment, ferrous-fortifier and Antianemic Agents, there is important physiological action and very high practical valence
Value, is widely used in food industry, medicines and health protection field, is the best biology of generally acknowledged at present absorptivity highest, effect
Chalybeate.
In conventional blood testing, other than detection blood glucose and cholesterol, ferroheme is also an important biochemistry
Examination criteria, and be also required to detect its content to control product quality, thus researcher in the industrialized production of ferroheme
Also increasingly pay attention to ferroheme detection.Currently, detection ferroheme method mainly have colorimetric method, ultraviolet spectrophotometry,
Electrochemical method and HPLC methods etc..But these methods have, and operating procedure is complicated, operating error is larger, measurement result accuracy
The problems such as low.
Invention content
Goal of the invention：In view of the problems of the existing technology, the present invention provides a kind of based on dendritic gold nano body structure surface
Enhance the detection method of the ferroheme of Raman spectrum, dendritic gold nano structure is applied to the detection of ferroheme by this method, is established
It is a kind of based on dendritic gold nano body structure surface enhancing Raman effect hematin test new method, the method can quickly,
Effective detection ferroheme, and have many advantages, such as high sensitivity, easy to operate, of low cost.
The present invention also provides a kind of detection methods for the ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface
Application.
Technical solution：It is above-mentioned purpose to realize, it is a kind of as described herein to be increased based on dendritic gold nano body structure surface
The detection method of the ferroheme of strong Raman spectrum, includes the following steps：
(1) dendritic gold nano structure-solution is prepared：By gold nano structure disperses in ethanol solution, gold nano structure is obtained
Dispersion liquid A；
(2) haemachrome solution is prepared：Ferroheme is dissolved in dimethyl sulfoxide (DMSO), dilution is used in combination, obtains various concentration
Haemachrome solution B；
(3) standard sample is prepared：Gold nano structure disperses liquid A and ferroheme mother liquor B is mixed, is then centrifuged for point
From removing supernatant, lower sediment thing be distributed in ethanol solution, dispersion liquid is taken to dry, obtain blood red containing various concentration
The standard sample of element；
(4) standard curve of dendritic gold nano structure detection ferroheme is established：Pass through Raman spectrometer detecting step (3) institute
The Raman spectrum of the standard sample of preparation, and record its Raman spectrum characteristic peak signal I；Finally by obtained I and step (3)
The natural number logarithm of the hemoglobin concentration of the standard sample of gained various concentration is depicted as I-ln c standard curves, using linear
The Return Law obtains I-ln c equations of linear regression, establishes the ferroheme of the surface-enhanced Raman effect based on dendritic gold nano structure
Detection method；
(5) concentration of ferroheme in sample to be tested is detected：Sample to be tested and the gold nano structure of preparation in step (1) is molten
Liquid is mixed, and is then centrifuged for detaching, removes supernatant, lower sediment thing is distributed in ethanol solution, dispersion liquid is taken to dry,
Its Raman spectrum is detected according to the identical method with step (4), according to the intensity I of Raman spectrum characteristic peak, by being obtained in step 4)
The standard curve arrived calculates the content of ferroheme in sample to be tested.
Wherein, step (1) the gold nano structure is dendritic, and particle size is 50~60nm.Existing skill may be used
Known method synthesis in art.Synthesis in relation to dendritic gold nanoparticle, reference can be made to document (Kumar, P.S.；Pastoriza-
Santos,I.；Rodríguez-González,B.；García de Abajo,F.J.；Liz-Marzán L.M.；High-
yield synthesis and optical response of gold nanostars,Nanotechnology,2008,
19,015606(6pp)。
Preferably, in the prepared gold nano structure disperses liquid A of step (1), a concentration of the 50 of dendritic gold nanoparticle
~150 μ g/mL.
Further, in the prepared haemachrome solution B of step (2), the molar concentration of ferroheme is 0.075~
1.50mmol/L。
Wherein, it is 12-24h that step (3) mixing, which is the magnetic agitation time,.
Further, the lower sediment thing is distributed to a concentration of 100~300 of dendritic gold nanoparticle in ethanol solution
μ g/mL, a concentration of 0.05~1.20mmol/L of the ferroheme of the standard sample of the various concentration ferroheme.
Preferably, the excitation wavelength of step (4) described Raman spectrometer is 514nm, power is 10mW, and the time of integration is
60ms。
Preferably, step (4) the middle Raman peaks selected at 1622nm wavelength are as quantitative analysis characteristic peak.
The detection method of the ferroheme of the present invention for being enhanced Raman spectrum based on dendritic gold nano body structure surface is being examined
Survey the application in the concentration of ferroheme in human erythrocyte.
Mechanism：The invention firstly uses the gold nanoparticles that seed mediated growth method is prepared for dendritic morphology；By itself and ferroheme
Effect acts on the surface-enhanced Raman of ferroheme by evaluating prepared dendritic gold nano structure, finds dendritic gold nano
Structure has good humidification to the Raman signal of ferroheme, a kind of using Surface enhanced Raman spectroscopy method pair to propose
The method that ferroheme carries out quantitative analysis.
The method of the present invention uses dendritic gold nano structure for Raman substrate, after gold nano structure is added, the drawing of ferroheme
Graceful signal can be remarkably reinforced, and wherein feature peak intensity is related with the content of ferroheme, to the dendritic gold nano knot
Structure is Raman substrate, and Surface enhanced Raman spectroscopy method may be used and carry out quantitative detection to ferroheme.Gold nano structure has only
Special electrical and optical properties and good biocompatibility, can be used safely in field of biological detection.
Advantageous effect：Compared with prior art, the present invention high ferro according to the present invention based on Surface enhanced Raman spectroscopy
Dendritic gold nano structure is applied to the detection of ferroheme by the detection method of ferroheme, is established a kind of based on dendritic gold nano knot
The new method of the hematin test of structure surface-enhanced Raman effect, the method can fast and effectively detect ferroheme, and
Has many advantages, such as high sensitivity, easy to operate, of low cost.
Specifically have the following advantages that：
1, dendritic gold nano structure used by the method for the present invention has more compared with the metal nanoparticle of other patterns
Strong surface-enhanced Raman effect, and there is better biocompatibility with ferroheme, it is more suitable for biochemistry detection.
2, in method of the invention, the dendritic gold nano structure of use need not be modified additionally, and valence need not be used
The organic or biochemical material that lattice are more expensive, Connection Step is cumbersome carries out pre-treatment, therefore easy to operate.
3, it is quick by Surface enhanced Raman spectroscopy ferroheme to may be implemented in the present invention, efficient to detect.
4, for the present invention to the detection method of ferroheme, the range of linearity is 0.05~1.20mmol/L；Detection is limited to
0.02mmol/L, detection range is wide, and detection limit is low.
Description of the drawings
Fig. 1 is the transmission electron microscope picture of dendritic gold nanoparticle prepared by the present invention；
Fig. 2 is the standard curve that hemoglobin concentration detects in the present invention.
Specific implementation mode
Below in conjunction with drawings and examples, the invention will be further described.
Embodiment 1
The preparation of dendritic gold nano structure and solution are prepared
1, the preparation of dendritic gold nano structure
The present embodiment synthesizes dendritic gold nano structure using seed mediated growth method.Specific method is：Weigh 5g polyvinyl pyrroles
Alkanone (PVP) is dissolved in 25mL n,N-Dimethylformamide (DMF), takes wherein 15mL PVP-DMF solution magnetic agitation, rapidly
The gold seeds of the chlorauric acid solution and 43 μ L PVP packages of 85 μ L 2% (wt.) are added, continue magnetic agitation 1h.Wait for that the reaction was complete
Afterwards, by product centrifuge washing, gold nano grain structure is dendritic morphology, and particle size is 50~60nm.
2, dendritic gold nano structure-solution is prepared：The dendritic gold nanoparticle of above-mentioned synthesis is dispersed in ethanol solution,
Gold nano structure-solution is obtained, wherein a concentration of 100 μ g/mL of dendritic gold nano structure.
3, haemachrome solution is prepared：Ferroheme is dissolved in DMSO, be used in combination dilution (volume ratio be 66.5% ethyl alcohol,
17% acetic acid and 16.5% pure water mixed liquor) dilution obtain a series of haemachrome solution of concentration, concentration is respectively
0.075mmol/L, 0.15mmol/L, 0.30mmol/L, 0.60mmol/L, 1.20mmol/L, 1.50mmol/L.
Embodiment 2
The surface-enhanced Raman evaluation of effect of dendritic gold nano structure
Dendritic gold nano structure is detected by Raman spectrometer to act on the surface-enhanced Raman of ferroheme；Wherein excitation wave
A length of 514nm, power 10mW, it is analysis object to choose the signal at wavelength 1622nm.It takes a concentration of in 20 μ L embodiments 1
The haemachrome solution of 1.20mmol/L drips on clean sheet glass, dries, measure its Raman spectrum；Take 2.0mL embodiments 1
The haemachrome solution mixing of the dendritic gold nano structure-solution and a concentration of 1.50mmol/L of 1.0mL of middle preparation, magnetic agitation
12h is centrifuged, is removed supernatant liquor, following sediment is distributed in 1.0mL ethanol solutions, ultrasonic disperse, wherein gold
A concentration of 200 μ g/mL of nanostructure, a concentration of 1.20mmol/L of ferroheme pipette wherein 20 μ L and drop in clean slide
On, it dries, its Raman spectrum is detected by Raman spectrometer.The result shows that after the 200 dendritic gold nanoparticles of μ g/mL are added,
The Raman signal of ferroheme is remarkably reinforced, and illustrates that dendritic gold nano structure makees ferroheme with good surface-enhanced Raman
With.
Embodiment 3
Establish the standard curve of dendritic gold nano structure detection hemoglobin concentration
The gold nano structure disperses liquid prepared in 2.0mL steps embodiment 1 and 1.0mL ferroheme mother liquors are taken respectively, are mixed,
Magnetic agitation 12h is centrifuged, is removed supernatant liquor, lower sediment thing is distributed in 1.0mL ethanol solutions, ultrasonic disperse,
A concentration of 200 μ g/mL of wherein dendritic gold nanoparticle, the concentration of ferroheme are respectively 0.05mmol/L, 0.10mmol/L,
0.25mmol/L, 0.50mmol/L, 1.00mmol/L, 1.20mmol/L,.It takes wherein 20 μ L to drip on clean sheet glass, dries in the air
It is dry, obtain the standard sample containing various concentration ferroheme；The Raman light of prepared standard sample is detected using Raman spectrometer
Spectrum records Raman spectrum characteristic peak signal I, and the natural number logarithm of obtained I and hemoglobin concentration are depicted as I-ln c marks
Directrix curve (as shown in Figure 2) uses linear regression method to obtain I-ln c equations of linear regression as I=731ln c-2235.Accordingly
Establish the standard curve of Raman signatures peak intensity and hemoglobin concentration natural number logarithm, the range of linearity is 0.05~
1.20mmol/L, detection are limited to 0.02mmol/L.
Embodiment 4
In order to verify a kind of detection of ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface of the invention
Method simulates a sample containing ferroheme under same experimental conditions.Take the prepared dendritic gold nano of 2.0mL embodiments 1
Structure and 1.0mL samples to be detected, mixing, magnetic agitation 12h centrifuge, remove supernatant liquor, by following sediment point
It is scattered in 1.0mL ethanol solutions, ultrasonic disperse；Wherein, a concentration of 200 μ g/mL of gold nano structure.Wherein 20 μ L are pipetted to drop in
On clean slide, dries, its Raman spectrum is detected by Raman spectrometer.According to the standard curve that embodiment 3 is established, calculate
It is about 0.83mmol/L to obtain content of hemachrome in sample to be tested, with ferroheme original concentration (0.80mmol/L) quite, by
This is as it can be seen that the detection method measurement result of the present invention is reliable.
Embodiment 5
The detection method of embodiment 5 is identical as embodiment 1-5, the difference is that：1 prepared gold nano knot of embodiment
In structure dispersion liquid, a concentration of 50 μ g/mL of dendritic gold nanoparticle；The mixed liquor magnetic agitation time is for 24 hours, to surpass in embodiment 2
A concentration of 100 μ g/mL of wherein dendritic gold nanoparticle after sound dispersion.
Embodiment 6
The detection method of embodiment 6 is identical as embodiment 1-5, the difference is that：1 prepared gold nano knot of embodiment
In structure dispersion liquid, a concentration of 150 μ g/mL of dendritic gold nanoparticle；The mixed liquor magnetic agitation time is 12h in embodiment 2, is surpassed
A concentration of 300 μ g/mL of wherein dendritic gold nanoparticle after sound dispersion.
Claims (9)
1. a kind of detection method for the ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface, which is characterized in that packet
Include following steps：
(1) dendritic gold nano structure-solution is prepared：By gold nano structure disperses in ethanol solution, gold nano structure disperses are obtained
Liquid A；
(2) haemachrome solution is prepared：Ferroheme is dissolved in dimethyl sulfoxide (DMSO), dilution is used in combination, obtains the blood red of various concentration
Plain solution B；
(3) standard sample is prepared：Gold nano structure disperses liquid A and ferroheme mother liquor B are mixed, are then centrifuged for detaching, is moved
Supernatant is removed, lower sediment thing is distributed in ethanol solution, dispersion liquid is taken to dry, obtains the mark containing various concentration ferroheme
Quasi- sample；
(4) standard curve of dendritic gold nano structure detection ferroheme is established：It is prepared by Raman spectrometer detecting step (3)
Standard sample Raman spectrum, and record its Raman spectrum characteristic peak signal I；Finally by obtained I and step (3) standard
The natural number logarithm of the hemoglobin concentration of sample is depicted as I-ln c standard curves, and I-ln c lines are obtained using linear regression method
Property regression equation, establish based on dendritic gold nano structure surface-enhanced Raman effect hematin test method；
(5) concentration of ferroheme in sample to be tested is detected：The gold nano structure-solution prepared in sample to be tested and step (1) is mixed
Stirring is closed, is then centrifuged for detaching, removes supernatant, lower sediment thing is distributed in ethanol solution, dispersion liquid is taken to dry, according to
Identical method detects its Raman spectrum with step (4), according to the intensity I of Raman spectrum characteristic peak, by what is obtained in step 4)
Standard curve calculates the content of ferroheme in sample to be tested.
2. a kind of detection of ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface according to claim 1
Method, which is characterized in that step (1) the gold nano structure is dendritic, and particle size is 50~60nm.
3. a kind of detection of ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface according to claim 1
Method, which is characterized in that in the prepared gold nano structure disperses liquid A of step (1), the concentration of dendritic gold nanoparticle is preferably
50~150 μ g/mL.
4. a kind of detection of ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface according to claim 1
Method, which is characterized in that in the prepared haemachrome solution B of step (2), the molar concentration of ferroheme is 0.075~
1.50mmol/L。
5. a kind of detection of ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface according to claim 1
Method, which is characterized in that it is 12-24h that step (3) mixing, which is the magnetic agitation time,.
6. a kind of detection of ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface according to claim 1
Method, which is characterized in that step (3) described lower sediment thing is distributed to a concentration of of dendritic gold nanoparticle in ethanol solution
100~300 μ g/mL, a concentration of 0.05~1.20mmol/L of the ferroheme of the standard sample of the various concentration ferroheme.
7. a kind of detection of ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface according to claim 1
Method, which is characterized in that the excitation wavelength of step (4) described Raman spectrometer is 514nm, and power is 10mW, and the time of integration is
60ms。
8. a kind of detection of ferroheme enhancing Raman spectrum based on dendritic gold nano body structure surface according to claim 1
Method, which is characterized in that step (4) the middle Raman peaks selected at 1622nm wavelength are as quantitative analysis characteristic peak.
9. a kind of detection method of the ferroheme described in claim 1 enhancing Raman spectrum based on dendritic gold nano body structure surface
Application in detecting human erythrocyte in the concentration of ferroheme.
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| CN112540071A (en) * | 2020-11-10 | 2021-03-23 | 江苏大学 | Rapid and ultrasensitive detection method for capsaicin in illegal cooking oil based on surface enhanced Raman spectroscopy combined with molecular derivatization technology |
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2018
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|---|---|---|---|---|
| WO2003042403A2 (en) * | 2001-11-14 | 2003-05-22 | Array Bioscience Corporation | Biochips for characterizing biological processes |
| CN104267017A (en) * | 2014-10-09 | 2015-01-07 | 吉林大学 | Heme protein functional magnetic surface enhanced Raman active substrate and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 饶世贤等: "基于NIR-SERS基底的柠檬酸钠、氯化血红素NIR-SERS光谱研究", 《光散射学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112540071A (en) * | 2020-11-10 | 2021-03-23 | 江苏大学 | Rapid and ultrasensitive detection method for capsaicin in illegal cooking oil based on surface enhanced Raman spectroscopy combined with molecular derivatization technology |
| CN112540071B (en) * | 2020-11-10 | 2022-08-23 | 江苏大学 | Rapid and ultrasensitive detection method for capsaicin in illegal cooking oil based on surface enhanced Raman spectroscopy combined with molecular derivatization technology |
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| CN108444978B (en) | 2020-09-18 |
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