CN108444966A - A kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner - Google Patents
A kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner Download PDFInfo
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- CN108444966A CN108444966A CN201810336844.1A CN201810336844A CN108444966A CN 108444966 A CN108444966 A CN 108444966A CN 201810336844 A CN201810336844 A CN 201810336844A CN 108444966 A CN108444966 A CN 108444966A
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- concentration gradient
- glass substrate
- mixed liquor
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- battle array
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention provides a kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner, including glass substrate and organic fluorescence mixed liquor, the organic fluorescence mixed liquor is uniformly distributed on a glass substrate in dot matrix, the organic fluorescence mixed liquor includes 488 fluorescent dyes of Alexa Fluor, Cy3 dCTP fluorescent dyes and dilution, the dilution is phosphate buffer or TE buffer solutions or aqueous solution, and chemical modification is done on the surface of the glass substrate or surface is coated with polymeric membrane.Organic fluorescence mixed liquor dot matrix on the glass substrate is made of 1 ~ 8 branch battle array, and each branch battle array is uniformly distributed and is arranged on glass substrate, the organic fluorescence mixed liquor drop of each branch battle array is in A rows × B row arrangements, the value range of the A and B is respectively 20 ~ 40 and 5 ~ 20, the beneficial effects of the present invention are：The concentration gradient fluorescent calibration piece makes simple, detection result protrusion, with short production cycle and of low cost.
Description
Technical field
The present invention relates to biochip production equipment technologies, are especially for calibration indigo plant, the green micro- battle array of double excitation
A kind of concentration gradient fluorescence of laser micro array chip scanner of the range of linearity of row chip scanner, sensitivity and resolving power
Corrector strip.
Background technology
With the rapid development of biotechnology, electronic technology and biotechnology are combined the brother for the semiconductor chip that has been born
Product --- biochip, this will bring a deepgoing revolution to our life.This revolution is held for global
Supervention exhibition can all play immeasurable contribution.Between China's biochip research starts from 1997-1998, although starting late,
But technology and industry development are rapid, realize interim breakthrough from scratch, and gradually develop, biochip is
From technical research and product development stage move towards technology apply and the product sales stage, chip of expression spectrum, major disease diagnose
Larger achievement is achieved in the relevant device development of chip and biochip.The biochip market of China in 2008 is about 100,000,000 U.S.s
Member, and just increased with 20% or more speed, biochip market is up to 900,000,000 dollars to 2020.Wherein, micro-array chip
Scanner is exactly for micro-array chip scanning, detecting instrument that is essential, playing effect of crucial importance.Due to sweeping
Light source, light path and the detecting system for retouching instrument will appear subtle variation with the extension of usage time, this often brings measurement to miss
Difference, and fluorescence detection is high to sensitivity, minimum detection error may cause completely opposite when in for trace detection
Conclusion, therefore prepare concentration gradient corrector strip and just seem extremely necessary for the calibration of micro-array chip scanner.
Currently, the material for being commonly used for fluorescent calibration mainly has organic fluorescence materials, polymer-doped organic fluorescence materials, height
Molecular material, inorganic material doping organic fluorescent dye, inorganic ions doping solid inorganic material etc..Inorganic fluorescent material makes
Corrector strip trivial operations, take it is longer, cost is higher, and organic fluorescence materials make corrector strip it is simple and fast, of low cost.With
The continuous expansion in biochip market, the demand of scanner is also increasing, this to make simple, low-cost corrector strip
It is more suitable for promoting and applying.
Invention content
The technical problem to be solved by the present invention is to：In view of the problems of the existing technology, a kind of laser micro array core is provided
The concentration gradient fluorescent calibration piece and preparation method of piece scanner, this method are easy to operate, with short production cycle and of low cost.
The technical problem to be solved in the present invention is realized using following technical scheme：A kind of laser micro array chip scanner
Concentration gradient fluorescent calibration piece, including glass substrate and organic fluorescence mixed liquor, the organic fluorescence mixed liquor is equal in dot matrix
On a glass substrate, the organic fluorescence mixed liquor includes 488 fluorescent dyes of Alexa Fluor, Cy3-dCTP fluorescent dyes to cloth
And dilution, the dilution are phosphate buffer or TE buffer solutions or aqueous solution.
Preferably, chemical modification is done on the surface of the glass substrate or surface is coated with polymeric membrane.
Preferably, the organic fluorescence mixed liquor dot matrix on the glass substrate is made of 1 ~ 8 branch battle array, and each branch
Battle array, which is uniformly distributed, to be arranged on glass substrate, and the organic fluorescence mixed liquor drop of each branch battle array is in A rows × B row arrangements, the A
Value range with B is respectively 20 ~ 40 and 5 ~ 20.
Preferably, the fluorescence signal value of the organic fluorescence mixed liquor drop of the branch battle array is with a line all same, and adjacent
Two rows change in multiple proportion.
Preferably, the fluorescence signal value is the 1st row in multiple proportion and the 1st row reciprocal is saturated fluorescence value, the 2nd row
The 2nd row fluorescence signal value of ~ inverse is in the increased linear relationship of positive number multiple proportions, and sensitivity is less than or equal to 13 rows.
Preferably, the coefficient R of the linear relationship2﹥ 0.99, that is, taking the median of every row fluorescence signal value to be used as should
Concentration single measurements are to the logarithm of induction signal median by abscissa, logarithm of the logarithm of line number corresponding concentration value
Ordinate is mapped, and the coefficient R for the linear relationship for obtaining curve is investigated2, and work as R2When ﹥ 0.99, meet the minimum of the range of linearity
The ratio of concentration and maximum concentration is greater than or equal to 103。
Preferably, it is corresponding to meet the minimum identifiable signal of the computing system in same scan image for the sensitivity
Concentration value, that is, minimum response is more than 10 with the ratio for the Cmin for reaching saturation signal3, i.e., the line number of minimum response is small
In or be equal to 13 rows, and equal to 13 row when requires signal to be greater than or equal to 2 to the ratio SNR of noise.
Preferably, also contain dimethyl sulphoxide solution in the dilution.
Preferably, the battle array spacing of the adjacent branch battle array is identical, and the point spacing of the branch battle array is identical and is greater than or equal to
400 microns.
Compared with prior art, the beneficial effects of the invention are as follows：The production method of corrector strip is simple and fast, the production cycle
Short, required making material and instrument is relatively fewer, of low cost, has stronger product competitiveness, with biochip city
The continuous expansion of field is suitble to marketing and application.
Description of the drawings
Fig. 1 is the scanning figure of the bluish-green fluorescence mixed liquor dot matrix of the concentration gradient fluorescent calibration piece of the present invention.
Fig. 2 is the scanning figure of the branch battle array of the bluish-green fluorescence of the concentration gradient fluorescent calibration piece of the present invention.
Fig. 3 is the initial concentration sample spot surface sweeping figure of the present invention.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with the accompanying drawings and specific implementation
The present invention is described in detail for example.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, not
For limiting the present invention.
One embodiment of the present of invention：31 concentration（Row）The preparation of × 10 row gradient corrector strips
A kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner, the corrector strip are that chemical modification is done on surface
Glass substrate or surface are coated with the glass substrate of polymeric membrane, and size is 75.6 × 25mm, is on the surface of corrector strip
Dot matrix arranges organic fluorescence mixed liquor, and the organic fluorescence mixed liquor includes 488 fluorescent dyes of Alexa Fluor, Cy3-dCTP
Fluorescent dye and dilution, the dilution are phosphate buffer or TE buffer solutions or aqueous solution.The Alexa Fluor
488 fluorescent dyes not only have brightness higher, but also have the characteristics that photostability is more preferable, 488 Hes of Alexa Fluor
Cy3-dCTP is played an important role in the assessment of the quantitative accuracy of gene expression after dilution.
As shown in Fig. 1 ~ Fig. 3, organic fluorescence mixed liquor develop the color on a glass substrate after state diagram.
Chemical modification is done on the surface of glass substrate or surface is coated with polymeric membrane, the organic fluorescence on the glass substrate
Mixed liquor dot matrix is made of 1 ~ 8 branch battle array, and each branch battle array is uniformly distributed and is arranged on glass substrate, each branch battle array
For organic fluorescence mixed liquor drop in A rows × B row arrangements, the value range of the A and B is respectively 20 ~ 40 and 5 ~ 20.It is described
The fluorescence signal value of the organic fluorescence mixed liquor drop of branch battle array is with a line all same, and adjacent rows change in multiple proportion.
The fluorescence signal value is the 1st row in multiple proportion and the 1st row reciprocal is saturated fluorescence value, the 2nd row ~ the 2nd row fluorescence of inverse
Signal value is in the increased linear relationship of positive number multiple proportions, and sensitivity is less than or equal to 13 rows.The related coefficient of the linear relationship
R2﹥ 0.99 takes the median of every row fluorescence signal value as the concentration single measurements, with the logarithm of line number corresponding concentration value
Value is that abscissa, logarithm are ordinate mapping to the logarithm of induction signal median, investigates the phase for the linear relationship for obtaining curve
Close coefficients R2, and work as R2When ﹥ 0.99, the ratio of the minimum concentration and maximum concentration that meet the range of linearity is greater than or equal to 103.Institute
It states sensitivity and meets the corresponding i.e. minimum response of concentration value of the identifiable signal of computing system minimum in same scan image
Value is more than 10 with the ratio for the Cmin for reaching saturation signal3, i.e., the line number of minimum response is less than or equal to 13 rows, and
Signal is required to be greater than or equal to 2 to the ratio SNR of noise when equal to 13 row.
Also contain dimethyl sulphoxide solution in dilution, the battle array spacing of the adjacent branch battle array is identical, the branch battle array
Point spacing is identical and is greater than or equal to 400 microns.
The glass substrate that the glass substrate of surface chemical modification or surface are coated with to polymeric membrane is horizontally arranged, in glass base
On piece sprays organic fluorescence mixed liquor, and the machine fluorescence mixed liquor arranges that the machine is glimmering on a glass substrate in the form of dot matrix
Light mixed liquor dot matrix is made of four branch battle arrays, and four branch battle arrays arrange setting on a glass substrate in 2 rows × 2, described every
A branch battle array is the column matrix of 31 rows × 10, in the organic fluorescent dye contained by with the machine fluorescence mixed liquor of a line in branch battle array
Concentration all same, since the 3rd row, often the contained organic fluorescent dye concentration of row is successively decreased successively, wherein the 1st row is organic
For fluorescence mixing drop as index marker, the 2nd row uses deionized water spraying as blank control, that is, completes for blue, green bidifly
The preparation of the concentration gradient piece of light micro-array chip scanner calibration measurement.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, it is noted that all
All any modification, equivalent and improvement made by within the spirit and principles in the present invention etc. should be included in the guarantor of the present invention
Within the scope of shield.
Claims (9)
1. a kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner, including glass substrate and organic fluorescence mixing
Liquid, the organic fluorescence mixed liquor are uniformly distributed on a glass substrate in dot matrix, it is characterised in that：The organic fluorescence mixed liquor includes
488 fluorescent dyes of Alexa Fluor, Cy3-dCTP fluorescent dyes and dilution, the dilution be phosphate buffer or
TE buffer solutions or aqueous solution.
2. a kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner, feature exist according to claim 1
In：Chemical modification is done on the surface of the glass substrate or surface is coated with polymeric membrane.
3. a kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner according to claim 1 or claim 2, feature
It is：Organic fluorescence mixed liquor dot matrix on the glass substrate is made of 1 ~ 8 branch battle array, and each branch battle array is uniformly distributed
It is arranged on glass substrate, the organic fluorescence mixed liquor drop of each branch battle array is in A rows × B row arrangements, the value of the A and B
Range is respectively 20 ~ 40 and 5 ~ 20.
4. a kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner, feature exist according to claim 3
In：The fluorescence signal value of the organic fluorescence mixed liquor drop of the branch battle array is with a line all same, and adjacent rows are closed in multiple
System's variation.
5. a kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner, feature exist according to claim 4
In：The fluorescence signal value is the 1st row in multiple proportion and the 1st row reciprocal is saturated fluorescence value, and the 2nd row ~ the 2nd row of inverse is glimmering
Optical signal value is in the increased linear relationship of positive number multiple proportions, and sensitivity is less than or equal to 13 rows.
6. a kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner, feature exist according to claim 5
In：The coefficient R of the linear relationship2﹥ 0.99 takes the median of every row fluorescence signal value as the concentration single measurement
Value is ordinate mapping to the logarithm of induction signal median by abscissa, logarithm of the logarithm of line number corresponding concentration value, examines
It examines to obtain the coefficient R of the linear relationship of curve2, and work as R2When ﹥ 0.99, meet the minimum concentration of the range of linearity and most highly concentrated
The ratio of degree is greater than or equal to 103。
7. a kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner, feature exist according to claim 6
In：The sensitivity meets the corresponding concentration value of the minimum identifiable signal of the computing system in same scan image, that is, minimum
Response is more than 10 with the ratio for the Cmin for reaching saturation signal3, i.e., the line number of minimum response is less than or equal to 13
Signal is required to be greater than or equal to 2 to the ratio SNR of noise when going, and being equal to 13 row.
8. according to a kind of concentration gradient fluorescent calibration of laser micro array chip scanner described in claim 1-7 any one
Piece, it is characterised in that：Also contain dimethyl sulphoxide solution in the dilution.
9. according to a kind of concentration gradient fluorescent calibration of laser micro array chip scanner described in claim 1-7 any one
Piece, it is characterised in that：The battle array spacing of the adjacent branch battle array is identical, and the point spacing of the branch battle array is identical and is greater than or equal to
400 microns.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810336844.1A CN108444966A (en) | 2018-04-16 | 2018-04-16 | A kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810336844.1A CN108444966A (en) | 2018-04-16 | 2018-04-16 | A kind of concentration gradient fluorescent calibration piece of laser micro array chip scanner |
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| CN108444966A true CN108444966A (en) | 2018-08-24 |
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Cited By (3)
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| CN110146477A (en) * | 2019-05-31 | 2019-08-20 | 成都博奥晶芯生物科技有限公司 | A kind of preparation method and its calibration method of concentration gradient calibration chip |
| CN110865058A (en) * | 2019-11-21 | 2020-03-06 | 成都博奥独立医学实验室有限公司 | Concentration gradient fluorescence calibration sheet of laser microarray chip scanner and calibration method |
| CN111638104A (en) * | 2020-02-11 | 2020-09-08 | 上海萨迦生物科技有限公司 | Fluorescence calibration sheet of biochip scanner |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110146477A (en) * | 2019-05-31 | 2019-08-20 | 成都博奥晶芯生物科技有限公司 | A kind of preparation method and its calibration method of concentration gradient calibration chip |
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| CN110865058A (en) * | 2019-11-21 | 2020-03-06 | 成都博奥独立医学实验室有限公司 | Concentration gradient fluorescence calibration sheet of laser microarray chip scanner and calibration method |
| WO2021098142A1 (en) * | 2019-11-21 | 2021-05-27 | 成都博奥独立医学实验室有限公司 | Concentration gradient fluorescence calibration sheet for laser microarray chip scanner and calibration method |
| JP2022535937A (en) * | 2019-11-21 | 2022-08-10 | チォンドウ キャピタルバイオ メディアブ リミテッド | Density Gradient Fluorescence Calibration Sheet and Calibration Method for Laser Microarray Chip Scanner |
| US20220307982A1 (en) * | 2019-11-21 | 2022-09-29 | Chengdu Capitalbio Mediab Ltd. | Concentration gradient fluorescence calibration sheet for laser microarray chip scanner and calibration method |
| JP7280390B2 (en) | 2019-11-21 | 2023-05-23 | チォンドウ キャピタルバイオ メディアブ リミテッド | Density Gradient Fluorescence Calibration Sheet and Calibration Method for Laser Microarray Chip Scanner |
| US11946868B2 (en) * | 2019-11-21 | 2024-04-02 | Chengdu Capitalbio Mediab Ltd | Concentration gradient fluorescence calibration sheet for laser microarray chip scanner and calibration method |
| CN111638104A (en) * | 2020-02-11 | 2020-09-08 | 上海萨迦生物科技有限公司 | Fluorescence calibration sheet of biochip scanner |
| CN111638104B (en) * | 2020-02-11 | 2023-12-29 | 上海萨迦生物科技有限公司 | Fluorescent calibration sheet of biochip scanner |
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