CN108441418B - Identification system of bacteria - Google Patents
Identification system of bacteria Download PDFInfo
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- CN108441418B CN108441418B CN201810253414.3A CN201810253414A CN108441418B CN 108441418 B CN108441418 B CN 108441418B CN 201810253414 A CN201810253414 A CN 201810253414A CN 108441418 B CN108441418 B CN 108441418B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
Abstract
The invention belongs to the technical field of bacteria identification, and particularly relates to a bacteria identification system which comprises an installation body, a power module, a reagent adding unit, a stirring unit and an identification box, wherein the installation body is provided with a first cavity, a second cavity and a third cavity; the identification box is positioned at the third position of the cavity and contains bacteria to be identified; the power module is positioned at the upper end of the mounting body and used for providing power for the stirring unit; the reagent adding unit is positioned at the first cavity and used for sequentially adding a plurality of reagents into the identification box, and at least 2 reagent adding units are arranged; the stirring unit is positioned at the second cavity and connected with the power module, the stirring unit is used for stirring bacteria and reagents to be identified in the identification box, and at least 2 stirring units are arranged; the invention improves the reaction speed when the bacteria and the reagent are mixed, and improves the efficiency of bacteria identification and the accuracy of cell identification.
Description
Technical Field
The invention belongs to the technical field of bacteria identification, and particularly relates to a bacteria identification system.
Background
The identification of bacteria is to examine bacteria in various aspects, and the bacteria obtained by isolated culture must be cultured to a pure degree without other microorganisms for systematic identification. The systematic identification is to detect the morphological structure, growth characteristics, antigenicity, pathogenicity and the like of pathogenic bacteria and determine the genus, species and type of the isolated bacteria by using known standard immune serum. The procedure for microbial identification is usually based on their morphology, growth, biochemical characteristics, etc. and finally based on the immunoserological examination of the antigen. Morphological examination of the morphology of various bacteria, is relatively stable under appropriate circumstances. However, environmental changes, such as changes in the culture medium conditions, the action of antibiotics and chemicals, etc., can cause the bacteria to develop irregular morphologies and defects and diversity in cell walls.
The existing bacteria identification device has high requirement on manual operation, poor identification accuracy, limited use, more defects and inconvenient operation.
In view of the above, the present invention provides an identification system for identifying bacteria by using reagents, which can improve the speed and accuracy of the bacteria identification process.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides a bacteria identification system which is mainly used for identifying and identifying bacteria and aims to improve the efficiency of a bacteria identification process. The invention improves the reaction speed when the bacteria and the reagent are mixed and the efficiency of bacteria identification by the mutual matching work of the power module and the stirring unit, and simultaneously, the invention effectively simplifies the step of manually adding the reagent when observing experimental phenomena by the mutual matching work of the reagent adding unit and the identification box, ensures the concentration of the attention of a user and improves the accuracy of cell identification.
The technical scheme adopted by the invention for solving the technical problems is as follows: the identification system comprises an installation body, a power module, a reagent adding unit, a stirring unit and an identification box, wherein the installation body is provided with a first cavity, a second cavity and a third cavity; the identification box is positioned at the third position of the cavity and contains bacteria to be identified; the power module is positioned at the upper end of the mounting body and used for providing power for the stirring unit; the reagent adding unit is positioned at the first cavity and used for sequentially adding a plurality of reagents into the identification box, and at least 2 reagent adding units are arranged; the stirring unit is located cavity two, and the stirring unit is connected with power module, and the stirring unit is used for stirring wait appraisal bacterium and reagent in the appraisal box, and the stirring unit is provided with 2 at least.
The power module comprises a first motor, a rotary table, a connecting rod, a first sliding block and a hollow guide rod, wherein the upper end of the mounting body is provided with a first sliding groove, the first sliding block is positioned in the first sliding groove, and the first sliding block is in sliding connection with the first sliding groove; the turntable is positioned at the upper end of the mounting body; the first motor is positioned beside the turntable and drives the turntable to rotate; one end of the connecting rod is hinged with the edge of the rotary table, and the other end of the connecting rod is hinged with the first sliding block; the mounting body is provided with a second sliding groove, and the second sliding groove and the first sliding groove are positioned on the same straight line; the hollow guide rod is positioned in the second sliding groove and fixedly connected with the first sliding block, and the lower end of the hollow guide rod is hollow; the first motor drives the hollow guide rod to slide up and down in the second sliding groove through the rotary disc, the connecting rod and the first sliding block. When the sliding guide rod device is used, the first motor is started, the first motor rotates to drive the rotating disc to rotate, the rotating disc drives the first sliding block to slide back and forth in the first sliding groove through the connecting rod, and the sliding block drives the hollow guide rod to slide up and down in the second sliding groove.
The reagent adding unit comprises a gas cylinder, a reagent bottle, a gas pipe and an automatic ventilation valve, a plurality of bottle clamps are arranged on the mounting body, the bottle clamps are positioned at the first cavity, and the bottle clamps are used for clamping the reagent bottle; the reagent bottle comprises a bottle body, a rubber air plug and a needle plug; the rubber air plug is arranged on the bottle body and seals the bottle body, a plurality of capillary holes are formed in the center of the rubber air plug, and the rubber air plug is airtight when no external air pressure acts on the rubber air plug; the needle plug is arranged at the lower end of the bottle body and seals the lower end of the bottle body, the needle head on the needle plug is a capillary needle head, and the reagent cannot flow out of the needle plug under no external pressure; the automatic ventilation valve is positioned between the gas cylinder and the gas pipe, one end of the automatic ventilation valve is communicated with the gas cylinder, and the other end of the automatic ventilation valve is communicated with the upper end of the gas pipe; the lower end of the air pipe is provided with a rubber sleeve which is sleeved at the upper end of the reagent bottle; the mounting body is provided with a plurality of reagent runners, one end of each reagent runner is located under the corresponding needle plug, and the other end of each reagent runner is communicated with the through hole formed in the hollow guide rod. During the use, start the automatic ventilation valve, the gaseous flow direction automatic ventilation valve in the gas cylinder, the automatic ventilation valve is regularly started, the automatic control time of ventilating makes the gas through the automatic ventilation valve be quantitative gas, and gas passes through the trachea and gets into the reagent bottle, and the reagent in the quantitative reagent bottle of quantitative gas compression, in the reagent runner was located from the syringe needle stopper, during reagent passed through the reagent runner and gets into hollow guide arm, quantitative reagent got into in the appraisal box from hollow guide arm.
The automatic ventilation valve comprises a controller, a magnetic ball, an electromagnet and a first spring, wherein the controller is positioned on the outer wall of the mounting body; an air guide hole is formed in one position of the cavity; the electromagnet is positioned at the upper part of the air guide hole, a vent hole is formed in the electromagnet, the electromagnet is connected with the controller through a lead, and the electromagnet is used for sucking the magnetic ball to enable the air guide hole to be in a ventilation state; the magnetic ball is positioned at the lower end of the air guide hole and used for blocking the air guide hole; the first spring is positioned between the electromagnet and the magnetic ball and is used for pressing the magnetic ball against the lower end of the air guide hole; the upper end of the gas guide hole is communicated with the gas cylinder, the lower end of the gas guide hole is communicated with the gas pipe, a flowmeter is arranged on the gas pipe and connected with the controller, and the flowmeter is used for detecting the flow of gas flowing through the gas pipe. When the automatic ventilation valve is used, the first spring supports the magnetic ball at the orifice of the gas guide hole, the automatic ventilation valve is in a closed state, after the automatic ventilation valve is started, the controller controls the electromagnet to adsorb the magnetic ball in a timing mode, the compression amount of the first spring compressed by the magnetic ball is increased, the magnetic ball leaves the orifice of the gas guide hole, the automatic ventilation valve is in a ventilation state, gas in the gas cylinder flows downwards through the vent hole in the electromagnet, the gas is subjected to flow timing, the flow meter meters the volume of the flowing gas and feeds the volume back to the controller, the controller analyzes and controls the electromagnet to electrify in a timing mode to adsorb the magnetic ball, the flow of the gas flowing through the automatic ventilation valve is controlled, and the gas enters the gas pipe after passing through the gas guide hole.
The stirring unit comprises a rotating shaft, a torsion spring, an elastic rope I, an elastic rope II, a return spring, a first shaft sleeve, a first shaft, a second shaft, a thrust ball bearing and a slide block II, wherein the rotating shaft is positioned in the cavity I and is rotatably connected with the hollow guide rod; one end of the torsion spring is connected with the rotating shaft, and the other end of the torsion spring is fixed on the inner wall of the cavity; a second sliding groove is formed in the mounting body, and an included angle of 20-45 degrees is formed between the second sliding groove and the vertical direction; the first shaft sleeve and the sliding groove are positioned on the same straight line; the second sliding block is positioned in the second sliding groove, the second sliding block is connected with the second sliding groove in a sliding mode, one end of the second sliding block is fixedly connected with the upper end of the second shaft, the other end of the second sliding block is connected with a return spring, and the return spring is positioned in the second sliding groove; one end of the first elastic rope is fixedly connected with the inner wall of the first cavity, and the other end of the first elastic rope is wound on the rotating shaft; one end of the second elastic rope is fixed on the rotating shaft, and the other end of the second elastic rope penetrates through the return spring and is fixed on the second sliding block; a thrust ball bearing is arranged between the first shaft and the second shaft, the lower end of the first shaft is connected with the upper end of the thrust ball bearing, the upper end of the second shaft is connected with the lower end of the thrust ball bearing, and the thrust ball bearing is used for realizing the relative rotation of the first shaft and the second shaft; a first spiral chute is arranged on the outer curved wall of the first shaft, and a stirring impeller is arranged at the tail end of the first shaft and used for stirring; the first shaft sleeve is internally provided with a first ball head, the first ball head is matched with the first spiral chute, the first shaft is connected with the first shaft sleeve in a rotating and sliding mode, and the first shaft moves up and down in the first shaft sleeve to realize forward and reverse rotation of the first shaft while moving up and down. When the stirring impeller is used, the hollow guide rod slides up and down in the sliding groove II, the hollow guide rod drives the rotating shaft to move up and down, when the rotating shaft moves up, the elastic rope I wound on the rotating shaft is pulled down, the rotating shaft rotates forwards, the torsional spring is wound with coexisting elastic potential energy, the elastic rope II pulls the sliding block II, the sliding block II drives the second shaft to move upwards, the second shaft drives the first shaft to move upwards at the first shaft sleeve through the thrust ball bearing, at the moment, the reset spring is compressed, when the first shaft moves upwards in the first shaft sleeve, the first shaft rotates forwards, and the first shaft rotates to drive the stirring impeller to rotate forwards; when the rotating shaft moves downwards, the torsion spring is scattered outwards and releases elastic potential energy, the rotating shaft rotates reversely and winds the first elastic rope, the second elastic rope is loosened, the reset spring resets, the second sliding block moves downwards and drives the second shaft to move downwards, the second shaft drives the first shaft to move downwards at the first shaft sleeve through the thrust ball bearing, the first shaft rotates reversely, and the first shaft drives the stirring impeller to rotate reversely; the first shaft moves up and down in the first shaft sleeve, so that the first shaft rotates forwards and backwards while moving up and down, and the stirring function of the stirring module is realized.
The invention has the beneficial effects that:
1. according to the bacteria identification system, the power module, the reagent adding unit, the stirring unit and the identification box are matched with one another to work, so that the functions of automatically adding the reagent to the identification box, automatically stirring the bacterial liquid and the reagent are realized, manual operation is reduced, and the bacteria identification efficiency and accuracy are improved.
2. According to the bacteria identification system, the power module and the stirring unit work in a matched mode, the power module drives the stirring module to mix and stir bacteria liquid and reagents, so that the bacteria liquid and the reagents can be mixed fast and fully, the reaction speed of the bacteria liquid and the reagents is increased, the bacteria identification speed is increased, and the bacteria identification efficiency is improved.
3. According to the bacteria identification system, the reagent adding unit and the identification box are matched with each other, the reagent adding unit can add the reagent to the identification box regularly and quantitatively, the trouble of manually adding the reagent is avoided, the concentration of the user in observing experimental phenomena is ensured, and the accuracy of cell identification is improved.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the connection of the automatic vent valve of the present invention to a reagent bottle;
FIG. 3 is a schematic view of the connection of a first shaft to a second shaft of the present invention;
FIG. 4 is a schematic view of the construction of a stirring impeller according to the invention;
in the figure: the device comprises an installation body 1, a first cavity 11, a second cavity 12, a third cavity 13, a bottle clamp 14, a power module 2, a first motor 21, a rotating disc 22, a first sliding block 23, a hollow guide rod 24, a stirring unit 3, an air bottle 31, a reagent bottle 32, a bottle body 321, an air pipe 33, an automatic ventilation valve 34, a controller 341, a magnetic ball 342, an electromagnet 343, a reagent adding unit 4, a rotating shaft 41, a torsion spring 42, a first elastic rope 43, a second elastic rope 44, a return spring 45, a first shaft sleeve 46, a first shaft 47, a second shaft 48, a thrust ball bearing 49 and an identification box 5.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described in the following combined with the specific embodiments.
As shown in fig. 1 to 4, a bacteria identification system is adopted in the identification method, the identification system comprises a mounting body 1, a power module 2, a stirring unit 3, a reagent adding unit 4 and an identification box 5, and a cavity I11, a cavity II 12 and a cavity III 13 are arranged on the mounting body 1; the identification box 5 is positioned at the third 13 position of the cavity, and bacteria to be identified are contained in the identification box 5; the power module 2 is positioned at the upper end of the mounting body 1, and the power module 2 is used for providing power for the reagent adding unit 4; the reagent adding unit 4 is positioned at the first cavity 11, the reagent adding unit 4 is used for sequentially adding a plurality of reagents to the identification box 5, and at least 2 reagent adding units 4 are arranged; stirring unit 3 is located cavity two 12 departments, and stirring unit 3 is connected with power module 2, and stirring unit 3 is used for stirring and appraises waiting to appraise bacterium and reagent in the box 5, and stirring unit 3 is provided with 2 at least.
The power module 2 comprises a first motor 21, a rotary table 22, a connecting rod, a first sliding block 23 and a hollow guide rod 24, the upper end of the mounting body 1 is provided with a first sliding groove, the first sliding block 23 is positioned in the first sliding groove, and the first sliding block 23 is in sliding connection with the first sliding groove; the rotary table 22 is positioned at the upper end of the mounting body 1; the first motor 21 is positioned beside the turntable 22, and the first motor 21 drives the turntable 22 to rotate; one end of the connecting rod is hinged with the edge of the rotary table 22, and the other end of the connecting rod is hinged with the first sliding block 23; the mounting body 1 is provided with a second sliding groove, and the second sliding groove and the first sliding groove are positioned on the same straight line; the hollow guide rod 24 is positioned in the second sliding groove, the hollow guide rod 24 is fixedly connected with the first sliding block 23, and the lower end of the hollow guide rod 24 is hollow; the first motor 21 drives the hollow guide rod 24 to slide up and down in the second sliding groove through the rotary disc 22, the connecting rod and the first sliding block 23. When the sliding type sliding device is used, the first motor 21 is started, the first motor 21 rotates to drive the rotating disc 22 to rotate, the rotating disc 22 drives the first sliding block 23 to slide back and forth in the first sliding groove through the connecting rod, and the first sliding block 23 drives the hollow guide rod 24 to slide up and down in the second sliding groove.
The reagent adding unit 4 comprises a gas cylinder 31, a reagent bottle 32, a gas pipe 33 and an automatic ventilation valve 34, a plurality of bottle clamps 14 are arranged on the mounting body 1, the bottle clamps 14 are located at the first cavity 11, and the bottle clamps 14 are used for clamping the reagent bottle 32; the reagent bottle 32 comprises a bottle body 321, a rubber air plug and a needle plug; the rubber air plug is arranged on the bottle body 321 and seals the bottle body 321, a plurality of capillary holes are formed in the center of the rubber air plug, and the rubber air plug is airtight when no external air pressure acts on the rubber air plug; the needle plug is arranged at the lower end of the bottle body 321 and seals the lower end of the bottle body 321, the needle on the needle plug head is a capillary needle, and the reagent cannot flow out of the needle plug head under no external pressure; the gas bottle 31 is positioned at the upper part of the first cavity 11, the gas pipe 33 is positioned at the upper end of the reagent bottle 32, the automatic ventilation valve 34 is positioned between the gas bottle 31 and the gas pipe 33, one end of the automatic ventilation valve 34 is communicated with the gas bottle 31, and the other end of the automatic ventilation valve 34 is communicated with the upper end of the gas pipe 33; the lower end of the air pipe 33 is provided with a rubber sleeve, and the rubber sleeve is sleeved at the upper end of the reagent bottle 32; a plurality of reagent flow channels are arranged on the mounting body 1, one end of each reagent flow channel is positioned right below the needle plug, and the other end of each reagent flow channel is communicated with a through hole arranged on the hollow guide rod 24. During the use, start automatic ventilation valve 34, the gaseous flow direction automatic ventilation valve 34 of gas cylinder 31, automatic ventilation valve 34 is regularly started, automatic control ventilation time, the gas that makes through automatic ventilation valve 34 is quantitative gas, and gas passes through trachea 33 entering reagent bottle 32, and the reagent in the quantitative reagent bottle 32 of quantitative gas compression, reagent follow needle stopper department runner reagent runner in, reagent passes through the reagent runner and gets into in the hollow guide arm 24, and quantitative reagent gets into in appraising box 5 from hollow guide arm 24.
The automatic ventilation valve 34 comprises a controller 341, a magnetic ball 342, an electromagnet 343 and a first spring, wherein the controller 341 is positioned on the outer wall of the mounting body 1; an air guide hole is formed in the first cavity 11; the electromagnet 343 and the first spring are positioned at the upper part of the air guide hole, the electromagnet 343 and the first spring are provided with air guide holes, the electromagnet 343 and the first spring are connected with the controller 341 through a lead, and the electromagnet 343 and the first spring are used for sucking the magnetic ball 342 to enable the air guide hole to be in an air guide state; the magnetic ball 342 is positioned at the lower end of the air guide hole, and the magnetic ball 342 is used for blocking the air guide hole; the first spring is positioned between the electromagnet 343 and the first spring and the magnetic ball 342, and the first spring is used for pressing the magnetic ball 342 against the lower end of the air guide hole; the upper end of air guide hole and gas cylinder 31 UNICOM, the lower extreme of air guide hole and trachea 33 UNICOM, and be provided with the flowmeter on the trachea 33, the flowmeter is connected with controller 341, and the flowmeter is used for detecting the gas flow who flows through trachea 33. When the automatic ventilation valve 34 is started, the controller 341 controls the electromagnet 343 and the spring I to adsorb the magnetic ball 342 at regular time, the compression amount of the spring I compressed by the magnetic ball 342 is increased, the magnetic ball 342 leaves the orifice of the gas guide hole, the automatic ventilation valve 34 is in a ventilation state, gas in the gas cylinder 31 flows downwards through the electromagnet 343 and the ventilation hole on the spring I, the gas flows through the flow meter, the volume of the flowing gas is measured by the flow meter and fed back to the controller 341, the controller 341 analyzes and controls the electromagnet 343 and the spring to be electrified at regular time to adsorb the magnetic ball 342, the flow of the gas flowing through the automatic ventilation valve 34 is controlled, and the gas enters the gas pipe 33 after passing through the gas guide hole.
The stirring unit 3 comprises a rotating shaft 41, a torsion spring 42, a first elastic rope 43, a second elastic rope 44, a return spring 45, a first shaft sleeve 46, a first shaft 47, a second shaft 48, a thrust ball bearing 49 and a second sliding block, wherein the rotating shaft 41 is positioned in the first cavity 11, and the rotating shaft 41 is rotatably connected with the hollow guide rod 24; one end of the torsion spring 42 is connected with the rotating shaft 41, and the other end of the torsion spring 42 is fixed on the inner wall of the first cavity 11; a second sliding groove is formed in the mounting body 1, and an included angle of 20-45 degrees is formed between the second sliding groove and the vertical direction; the first shaft sleeve 46 and the sliding groove are positioned on the same straight line; the second sliding block is positioned in the second sliding groove and is in sliding connection with the second sliding groove, one end of the second sliding block is fixedly connected with the upper end of the second shaft 48, the other end of the sliding block is connected with a return spring 45, and the return spring 45 is positioned in the second sliding groove; one end of the first elastic rope 43 is fixedly connected with the inner wall of the first cavity 11, and the other end of the first elastic rope 43 is wound on the rotating shaft 41; one end of the second elastic rope 44 is fixed on the rotating shaft 41, and the other end of the second elastic rope 44 penetrates through the reset spring 45 and is fixed on the second sliding block; a thrust ball bearing 49 is arranged between the first shaft 47 and the second shaft 48, the lower end of the first shaft 47 is connected with the upper end of the thrust ball bearing 49, the upper end of the second shaft 48 is connected with the lower end of the thrust ball bearing 49, and the thrust ball bearing 49 is used for realizing the relative rotation of the first shaft 47 and the second shaft 48; a first spiral sliding groove is formed in the outer curved wall of the first shaft 47, and a stirring impeller is arranged at the tail end of the first shaft 47 and used for stirring; the inner wall of the first shaft sleeve 46 is provided with a first ball head, the first ball head is matched with the first spiral chute, the first shaft 47 is connected with the first shaft sleeve 46 in a rotating and sliding manner, and the first shaft 47 moves up and down in the first shaft sleeve 46 to realize the forward and reverse rotation of the first shaft 47 while moving up and down. When the stirring impeller is used, the hollow guide rod 24 slides up and down in the second sliding groove, the hollow guide rod 24 drives the rotating shaft 41 to move up and down, when the rotating shaft 41 moves up, the first elastic rope 43 wound on the rotating shaft 41 is pulled down, the rotating shaft 41 rotates forwards, the torsion spring 42 winds up and stores elastic potential energy, the second elastic rope 44 pulls up the second sliding block, the second sliding block drives the second shaft 48 to move upwards, the second shaft 48 drives the first shaft 47 to move upwards at the first shaft sleeve 46 through the thrust ball bearing 49, at the moment, the return spring 45 is compressed, when the first shaft 47 moves upwards in the first shaft sleeve 46, the first shaft 47 rotates forwards, and the first shaft 47 rotates to drive the stirring impeller 371 to rotate forwards; when the rotating shaft 41 moves downwards, the torsion spring 42 disperses and releases elastic potential energy, the rotating shaft 41 rotates reversely and winds the elastic rope I43, the elastic rope II 44 looses, the return spring 45 resets, the sliding block II moves downwards and drives the second shaft 48 to move downwards, the second shaft 48 drives the first shaft 47 to move downwards at the first shaft sleeve 46 through the thrust ball bearing 49, the first shaft 47 rotates reversely, and the first shaft 47 rotates to drive the stirring impeller 371 to rotate reversely; the first shaft 47 moves up and down in the first bushing 46, so that the first shaft 47 rotates forward and backward while moving up and down, thereby realizing the stirring function of the stirring module.
The specific operation flow is as follows:
firstly, placing the reagent bottle 32 into the bottle clamp 14 for clamping, enabling the needle plug of the reagent bottle 32 to face downwards and be aligned with the reagent flow channel, and sheathing one end of the rubber air plug of the reagent bottle 32 by a rubber sleeve; placing the identification box 5 filled with the bacterial liquid to be detected into the third cavity 13 of the identification system, and enabling the identification box 5 to be positioned under the hollow guide rod 24;
starting the first motor 21, driving the rotating disc 22 to rotate by the rotation of the first motor 21, driving the first slider 23 to slide back and forth in the first sliding groove through the connecting rod by the rotating disc 22, driving the hollow guide rod 24 to slide up and down in the second sliding groove by the first slider 23, driving the rotating shaft 41 to move up and down by the hollow guide rod 24, when the rotating shaft 41 moves up, pulling down the first elastic rope 43 wound on the rotating shaft 41, rotating the rotating shaft 41 forwards, winding and accumulating elastic potential energy in the torsion spring 42, pulling up the second slider by the second elastic rope 44, driving the second shaft 48 to move up, driving the first shaft 47 to move up at the first shaft sleeve 46 by the second shaft 48 through the thrust ball bearing 49, compressing the return spring 45, when the first shaft 47 moves up in the first shaft sleeve 46, driving the first shaft 47 to rotate forwards, and driving the stirring impeller 371 to rotate forwards; when the rotating shaft 41 moves downwards, the torsion spring 42 disperses and releases elastic potential energy, the rotating shaft 41 rotates reversely and winds the elastic rope I43, the elastic rope II 44 looses, the return spring 45 resets, the sliding block II moves downwards and drives the second shaft 48 to move downwards, the second shaft 48 drives the first shaft 47 to move downwards at the first shaft sleeve 46 through the thrust ball bearing 49, the first shaft 47 rotates reversely, and the first shaft 47 rotates to drive the stirring impeller 371 to rotate reversely; the first shaft 47 moves up and down in the first shaft sleeve 46, so that the first shaft 47 rotates forwards and backwards while moving up and down, and the stirring function of the stirring module is realized;
the automatic ventilation valve 34 is started, the gas in the gas cylinder 31 flows to the automatic ventilation valve 34, the first spring presses the magnetic ball 342 against the orifice of the gas guide hole, the automatic ventilation valve 34 is in a closed state, after the automatic ventilation valve 34 is started, the controller 341 controls the electromagnet 343 and the first spring to adsorb the magnetic ball 342 at regular time, the compression amount of the first spring compressed by the magnetic ball 342 is increased, the magnetic ball 342 leaves the orifice of the gas guide hole, the automatic ventilation valve 34 is in a ventilation state, the gas in the gas cylinder 31 flows downwards through the vent holes on the electromagnet 343 and the first spring, the gas flows through the flow meter, the volume of the flowing gas is measured by the flow meter and fed back to the controller 341, the controller 341 analyzes and controls the electromagnet 343 and the spring 342 to be electrified at regular time to adsorb the magnetic ball and accordingly control the flow rate of the gas flowing through the automatic ventilation valve 34, the gas enters the gas pipe 33 after passing through the gas guide hole, the gas enters the reagent bottle 32 through the gas pipe 33, the quantitative gas compresses the reagent in the quantitative reagent bottle 32, the reagent flows into the reagent flow channel from the needle plug, the reagent enters the hollow guide rod 24 through the reagent flow channel, and the quantitative reagent enters the identification box 5 from the hollow guide rod 24.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and are only illustrative of the principles of the present invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A system for identifying bacteria, comprising: the reagent box comprises an installation body (1), a power module (2), a stirring unit (3), a reagent adding unit (4) and an identification box (5), wherein a first cavity (11), a second cavity (12) and a third cavity (13) are arranged on the installation body (1); the identification box (5) is positioned at the third cavity (13), and bacteria to be identified are contained in the identification box (5); the power module (2) is positioned at the upper end of the mounting body (1), and the power module (2) is used for providing power for the reagent adding unit (4); the reagent adding unit (4) is positioned at the first cavity (11), the reagent adding unit (4) is used for sequentially adding a plurality of reagents to the identification box (5), and at least 2 reagent adding units (4) are arranged; stirring unit (3) are located cavity two (12) department, and stirring unit (3) are connected with power module (2), and stirring unit (3) are used for stirring and appraise waiting to appraise bacterium and reagent in box (5), and stirring unit (3) are provided with 2 at least.
2. A system for identifying bacteria according to claim 1, wherein: the power module (2) comprises a motor I (21), a rotary table (22), a connecting rod, a sliding block I (23) and a hollow guide rod (24), a sliding groove I is arranged at the upper end of the mounting body (1), the sliding block I (23) is located in the sliding groove I, and the sliding block I (23) is in sliding connection with the sliding groove I; the turntable (22) is positioned at the upper end of the mounting body (1); the first motor (21) is positioned beside the turntable (22), and the first motor (21) drives the turntable (22) to rotate; one end of the connecting rod is hinged with the edge of the rotary table (22), and the other end of the connecting rod is hinged with the first sliding block (23); a second sliding groove is formed in the mounting body (1), and the second sliding groove and the first sliding groove are located on the same straight line; the hollow guide rod (24) is positioned in the second sliding groove, the hollow guide rod (24) is fixedly connected with the first sliding block (23), and the lower end of the hollow guide rod (24) is hollow; the first motor (21) drives the hollow guide rod (24) to slide up and down in the second sliding groove through the rotary table (22), the connecting rod and the first sliding block (23).
3. A system for identifying bacteria according to claim 2, wherein: the reagent adding unit (4) comprises an air bottle (31), a reagent bottle (32), an air pipe (33) and an automatic ventilation valve (34), a plurality of bottle clamps (14) are arranged on the mounting body (1), the bottle clamps (14) are located in the first cavity (11), and the bottle clamps (14) are used for clamping the reagent bottle (32); the reagent bottle (32) comprises a bottle body, a rubber air plug and a needle plug; the rubber air plug is arranged on the bottle body (321) and seals the bottle body (321), and the needle plug is arranged at the lower end of the bottle body (321) and seals the lower end of the bottle body (321); the gas bottle (31) is positioned at the upper part of the first cavity (11), the gas pipe (33) is positioned at the upper end of the reagent bottle (32), the automatic ventilation valve (34) is positioned between the gas bottle (31) and the gas pipe (33), one end of the automatic ventilation valve (34) is communicated with the gas bottle (31), and the other end of the automatic ventilation valve (34) is communicated with the upper end of the gas pipe (33); the lower end of the air pipe (33) is provided with a rubber sleeve, and the rubber sleeve is sleeved at the upper end of the reagent bottle (32); the mounting body (1) is provided with a plurality of reagent flow channels, one end of each reagent flow channel is positioned under the needle plug, and the other end of each reagent flow channel is communicated with a through hole formed in the hollow guide rod (24).
4. A system for identifying bacteria according to claim 3, wherein: the automatic ventilation valve (34) comprises a controller (341), a magnetic ball (342), an electromagnet (343) and a first spring, wherein the controller (341) is positioned on the outer wall of the mounting body (1); an air guide hole is formed in the first cavity (11); the electromagnet (343) and the first spring are positioned at the upper part of the air guide hole, vent holes are formed in the electromagnet (343) and the first spring, the electromagnet (343) and the first spring are connected with the controller (341) through leads, and the electromagnet (343) and the first spring are used for sucking the magnetic ball (342) to enable the air guide hole to be in a ventilation state; the magnetic ball (342) is positioned at the lower end of the air guide hole, and the magnetic ball (342) is used for blocking the air guide hole; the first spring is positioned between the electromagnet (343) and the first spring and the magnetic ball (342), and the first spring is used for pressing the magnetic ball (342) to the lower end of the air guide hole; the upper end and the gas bottle (31) UNICOM of air guide hole, the lower extreme and trachea (33) UNICOM of air guide hole, and be provided with the flowmeter on trachea (33), the flowmeter is connected with controller (341), and the flowmeter is used for detecting the gas flow who flows through trachea (33).
5. A system for identifying bacteria according to claim 2, wherein: the stirring unit (3) comprises a rotating shaft (41), a torsion spring (42), an elastic rope I (43), an elastic rope II (44), a return spring (45), a first shaft sleeve (46), a first shaft (47), a second shaft (48), a thrust ball bearing (49) and a sliding block II, wherein the rotating shaft (41) is positioned in the cavity I (11), and the rotating shaft (41) is rotatably connected with the hollow guide rod (24); one end of the torsion spring (42) is connected with the rotating shaft (41), and the other end of the torsion spring (42) is fixed on the inner wall of the first cavity (11); a second sliding groove is formed in the mounting body (1), and an included angle of 20-45 degrees is formed between the second sliding groove and the vertical direction; the first shaft sleeve (46) and the sliding groove are positioned on the same straight line; the second sliding block is positioned in the second sliding groove and is in sliding connection with the second sliding groove, one end of the second sliding block is fixedly connected with the upper end of the second shaft (48), the other end of the sliding block is connected with a return spring (45), and the return spring (45) is positioned in the second sliding groove; one end of the first elastic rope (43) is fixedly connected with the inner wall of the first cavity (11), and the other end of the first elastic rope (43) is wound on the rotating shaft (41); one end of the second elastic rope (44) is fixed on the rotating shaft (41), and the other end of the second elastic rope (44) penetrates through the return spring (45) and is fixed on the second sliding block; a thrust ball bearing (49) is arranged between the first shaft (47) and the second shaft (48), the lower end of the first shaft (47) is connected with the upper end of the thrust ball bearing (49), and the upper end of the second shaft (48) is connected with the lower end of the thrust ball bearing (49); a first spiral chute is arranged on the outer curved wall of the first shaft (47), a stirring impeller (371) is arranged at the tail end of the first shaft (47), and the stirring impeller (371) is used for stirring; the inner wall of the first shaft sleeve (46) is provided with a first ball head, the first ball head is matched with the first spiral sliding groove, and the first shaft (47) is connected with the first shaft sleeve (46) in a rotating and sliding mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810253414.3A CN108441418B (en) | 2018-03-26 | 2018-03-26 | Identification system of bacteria |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810253414.3A CN108441418B (en) | 2018-03-26 | 2018-03-26 | Identification system of bacteria |
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| CN108441418B true CN108441418B (en) | 2021-11-30 |
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| JP3601823B2 (en) * | 2002-05-23 | 2004-12-15 | 英治 吉田 | Fluid extraction device, fluid extraction method and fluid extraction unit |
| CN1768150A (en) * | 2003-04-07 | 2006-05-03 | 拜奥默里克斯公司 | Method for the detection and/or identification of bacteria present in a sample |
| WO2011035304A3 (en) * | 2009-09-21 | 2011-08-04 | Pocared Diagnostics Ltd. | System for conducting the identification of bacteria in biological samples |
| CN102325894A (en) * | 2008-12-31 | 2012-01-18 | 3M创新有限公司 | Coliform detection process and kit for use therein |
| CN103923830A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Microenvironment improvement method and system for bioactive substance extractor |
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2018
- 2018-03-26 CN CN201810253414.3A patent/CN108441418B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3601823B2 (en) * | 2002-05-23 | 2004-12-15 | 英治 吉田 | Fluid extraction device, fluid extraction method and fluid extraction unit |
| CN1768150A (en) * | 2003-04-07 | 2006-05-03 | 拜奥默里克斯公司 | Method for the detection and/or identification of bacteria present in a sample |
| CN102325894A (en) * | 2008-12-31 | 2012-01-18 | 3M创新有限公司 | Coliform detection process and kit for use therein |
| WO2011035304A3 (en) * | 2009-09-21 | 2011-08-04 | Pocared Diagnostics Ltd. | System for conducting the identification of bacteria in biological samples |
| CN103923830A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Microenvironment improvement method and system for bioactive substance extractor |
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