CN108420846A - Composition for protecting liver and preventing liver fibrosis from being formed and preparation method - Google Patents
Composition for protecting liver and preventing liver fibrosis from being formed and preparation method Download PDFInfo
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Abstract
The invention discloses a kind of compositions for protecting liver and preventing liver fibrosis from being formed, and are made of by weight major ingredient extract of Radix Ginseng stem and leaf 20 40%, milk thistle extract 20 35%, excessive sheep extract 10 20%, Astragalus Root P.E 10 20% and auxiliary material plant amylum 10 20%.The ginseng of extract of Radix Ginseng stem and leaf fictitious hosts costliness in the present invention, cooperation Herba Silybi mariani flavone, epimedium flavone, astragalus root total saponin more play raising and are immunized, and increase the effect of Liver protection wound and prevent the formation of liver fibrosis.It is suitable for various hepatitis, the health care of cirrhotic population and supplies treatment, the composition is convenient, hygienic, safe.The invention also discloses a kind of preparation methods of the composition for protecting liver and preventing liver fibrosis from being formed.
Description
Technical field
The composition and preparation method that the present invention relates to a kind of for protecting liver and preventing liver fibrosis from being formed.
Background technology
Hepatopathy is the common disease frequently-occurring disease in China, wide-scale distribution due to virus hepatitis, long-term heavy drinking in China
The phenomenon that it is much, cause liver fibrosis, hepatic sclerosis become one of common disease and the main vital cause of disease.Currently, the whole nation is every
Year, there are about more than 10 ten thousand people to die of hepatic sclerosis disease, its morbidity and mortality is in rising trend in recent years.What is occurred in the market is big
Most liver protecting health care improvement unobvious, absorption of human body effect is bad, and products material purity is inadequate, and formula is unreasonable,
It is of high cost.
Invention content
The purpose of the invention is to overcome the shortcomings of the prior art, and provide a kind of fine with Liver protection and anti-liver
The composition and preparation method for protecting liver and preventing liver fibrosis from being formed of dimensionization effect.
To achieve the purpose of the present invention, the technical scheme is that：
A kind of composition for protecting liver and preventing liver fibrosis from being formed is contained by following raw materials percentage by weight and is formed：
The excessive sheep extract 10-20% of extract of Radix Ginseng stem and leaf 20-40% milk thistle extracts 20-35%
Astragalus Root P.E 10-20% plant amylums 10-20%.
It preferably constitutes：25% milk thistle extract of extract of Radix Ginseng stem and leaf, 25% Shorthorned Epimedium P.E 20%
15% plant amylum 15% of Astragalus Root P.E.
Further, the extract of Radix Ginseng stem and leaf is made as follows：After gen-seng haulms are dried, by powder
Broken, the water measured using 15 times is solvent, and three times with the extraction of 95% ethyl alcohol, extraction time is respectively 3h, 3h and 2h；Extracting solution sheet frame mistake
Filter merges extracting solution and stands 24 hours, takes supernatant, when sodium hydroxide being added to adjust pH value to 10~12, through macroporous resin adsorption,
Impurity elimination is washed with water, alcohol-water elutes general ginsenoside, concentrate eluant, decoloration, recycling ethyl alcohol to no alcohol；Continue concentration and evaporation
Water is to proportion d=1.15；Spray drying, pulverizes and sieves.
Further, the milk thistle extract is made as follows：Milk thistle herb medicinal material coarse powder is broken, it crushes
8 times of amount hexanes reflux degreasings of medicinal material, are extracted 3 times with 12 times of 75% alcohol refluxs of amount after being vaporized hexane, 2 hours every time, are merged
Extracting solution recycles ethyl alcohol, and milk thistle ethanol extract is concentrated under reduced pressure to obtain；Milk thistle ethanol extract adds 80 DEG C of hot water dissolvings,
After cooling with hydrochloric acid tune PH to 2~3,12 hours are stood to filter, 2.5 kg HPD427 macroporous resin adsorptions of filtrate, first
It is rinsed to colourless with purified water, discards eluent, then with 2% sodium bicarbonate solution 9 L elutions, and add 16 L purified waters continuous
It washes, collects alkali and water continues washing lotion；5% hydrochloric acid solution tune pH to 2~4 of eluent is adsorbed with the polyamide of 6 L, then used
70% ethyl alcohol, 10 L elutions, collect alcohol eluen, are concentrated under reduced pressure, and it is total to obtain the milk thistle that concentration is about 60% or so for vacuum drying
Flavones.
Further, the Shorthorned Epimedium P.E is made as follows：Take epimedium herb, 15 times of volumes of dosing material
75% ethyl alcohol decoct refluxing extraction 3 hours, filtration, filtrate is spare；The dregs of a decoction add 75% alcohol reflux 2 hours of 13 times of volumes again,
Filtration, filtrate are spare；The dregs of a decoction add 75% alcohol reflux of 10 times of volumes to extract 2 hours again, filtration, and filtrate is spare；Merge 3 times and carries
Liquid is taken, relative density d=1.10~1.20 (50~60 DEG C) concentrate is concentrated under reduced pressure into, the water of 2 times of volumes is added in concentrate
Make dissolving, place about 4~6 hours, filtering, it is colourless, flow velocity that filtrate, which is first washed with water by D-101 types macroporous resin column to washing lotion,
It is 45ml/ points, discards water elution, then with 95% ethyl alcohol (4~5 times of amount bed volumes) elution, collect eluent, recycle ethyl alcohol
And relative density d=1.10~1.20 (50~60 DEG C) are concentrated into, it dry, pulverize, obtain barren wort total chromocor.
Further, the Astragalus Root P.E is made as follows：Milkvetch Root is placed in extractor with 75% second
Alcohol reflux extracts 2 times, the 1st time plus 10 times amount alcohol refluxs extractions, 3 hours, the 2nd time plus 8 times amount alcohol refluxs and extracts 2 hours,
Merge extracting solution, ethyl alcohol is recovered under reduced pressure, is concentrated into 1:1；The hot water dissolving for adding double quantity, stands overnight, filtering；Filtrate is used
D101 large pore resin absorption column upper props, filtrate adsorbed by macroporous absorbent resin after first with the distillation water elution of 4 times of column volumes, to remove
Carbohydrate content is removed, after with 70% ethanol elution of 4 times of column volumes, collects 70% ethanol eluate, decompression is concentrated to give the total soap of Radix Astragali
Glycosides.
A kind of preparation method of the composition as described above for protecting liver and preventing liver fibrosis from being formed, including under
Row step：
1. extract of Radix Ginseng stem and leaf that is dry, crushing, milk thistle extract, Shorthorned Epimedium P.E, Astragalus Root P.E are weighed according to quantity,
It is placed in blender, stirs evenly with plant amylum；
2. the material of mixing is packed by dosage form.
Further, the combination dosage form is granule, capsule, tablet or powder.
In the present invention, ginseng (Panax ginseng) is Araliaceae (Araliaceae) Panax (Panax) perennial grass
This plant, the ground such as three provinces in the northeast of China of main product China and the external Korea peninsula, Fukushima, Japan, former Soviet Union's East Siberia.Ginseng is
The traditional rare Chinese medicine in China has the laudatory title of " hundred herbal medicine kings ", mild-natured, sweet in flavor, slight bitter, tepor to reinforce vital energy, and multiple arteries and veins is solid
It is de-, it reinforces the spleen to benefit the lung, promotes the production of body fluid to quench thirst, the effect of tranquilize the mind and promote the intelligence.Ginseng is applied to tcm clinical practice and has more than 2000 years history, due to
It is paid attention to extensive pharmacological action and medical application by lot of domestic and foreign scholar.The principle active component of ginseng is behaved
Join saponin(e, in recent years, chemistry, pharmacology and clinical research prove, the pharmacological action of ginsenoside contained by gen-seng haulms and ginseng
Root saponin(e is essentially identical, and total saponin content is apparently higher than ginseng, about 6~12%.Currently, from gen-seng haulms first
After obtain nearly 60 saponins compounds, mainly include ginsenoside Rb 1, Rb 2, Rc, Rd, Rd 2, Re, F 1, F 2
、F 3 、F 4 、Rg 1 、Rg 2 、Rg 3 、Rg 4 、Rg 7 、Rh 1 、Rh 3 、Rh 4 、Rh 5 、Rh 6 、Rh 7 、
Rh 8, Rh 9, Ib etc..Ginsenoside has a variety of pharmacological activity, such as resisting cardiovascular systemic disease, anti-senile dementia, resists
The pharmacological actions such as diabetes, strengthen immunity, antifatigue, antitumor, antibacterial, antiviral, inhibition Apoptosis.Ginsenoside Re
It is two kinds of saponin(es common in gen-seng haulms with Rg 1, has been able to isolate and purify to obtain two kinds of soaps from gen-seng haulms at present
The monomer of glycosides, but preparation difficulty is larger, the process is more complicated, is not suitable for industrialized production, also just without practical popularization and application
Value.
Production has gen-seng chewing tablet at present, and it is total to mostly use ginseng slice, ginseng powder and ginseng extraction ginseng greatly
The methods of saponin(e, production cost is high, active ingredient absorption difference of the human body to ginseng.General ginsenoside used in the present invention is to pass through people
Join the product of cauline leaf extraction, past gen-seng haulms are thrown away by discarded, become the rubbish of pollution environment, the present invention makes full use of locality
Waste resource, reduce entreprise cost, clean social environment, have preferable economic benefit, social benefit and Ecological Effect
Benefit.It is proved through pharmacological research, ginsenoside is in treatment liver cancer, liver fibrosis, hepatic injury, hepatitis, hepatic ischemia reperfusion, fat
Have extensive pharmacological action in the diseases such as liver, mechanism of action is predominantly anti-oxidant, it is anti-inflammatory, inhibit CYP450 and promote liver
Several aspects such as cytothesis.
For milk thistle extensively using being Liver protection and anti-hepatic fibrosis medicines, active ingredient is Flavonoid substances, milk thistle
Element is a kind of antioxidant, can reduce the generation of free radical, anti-lipid peroxidation reaction, the work with anti-fibrosis
Property, there is protective effect to liver plasma membrane, moreover it is possible to be played by blocking on toxin and liver plasma membrane the combination of receptor antiviral
Effect.Silymarin energy directly Scavenger of ROS (ROS) maintains the mobility of cell membrane to anti peroxidation of lipid.To liver
Damaging agents such as ROS, hydroxy radical, hypochlorous acid anion, hydrogen peroxide etc. have the reactivity of height, can be with unsaturation
Aliphatic acid effect prevents liver plasma membrane damage caused by peroxidatic reaction of lipid.Silymarin also has anti-reduced glutathione
(GSH) emptying plays Hepatocyte protection.GSH levels are then in a slight decrease, illustrate that kidney is the main portions of blood plasma GSH degradations.
Silymarin protects liver plasma membrane, and silymarin removes, and there is resisting oxidation free radical, anti peroxidation of lipid to maintain the stream of cell membrane
Outside the effects that dynamic property, protection liver plasma membrane, moreover it is possible to block mycotoxin phalloidine etc. and specific receptors on liver plasma membrane
Combination, inhibit its attack and transmembrane transport to liver plasma membrane, interrupt its intestines liver cycle, enhancing liver plasma membrane to a variety of
The resistance of damage factor.The effect of anti hepatic fibrosis of silymarin is apparent, so that liver collagen content is declined 30%, and think this
One effect is related with its anti-oxidant and stabilizing cell membrane effect.Since it has preferable effect to various hepatopathys, make at present
Being developed with milk thistle and being applied to clinical drug has milk thistle capsule, legalon etc..
Epimedium active constituent is also Flavonoid substances, and barren wort total chromocor has extensive pharmacological activity, such as to painstaking effort
Guard system, central nervous system, hematological system, immune system, anti-inflammatory, anti-osteoporosis, anti-aging, antitumor etc..Herba Epimedii
General flavone has anti-oxidant and enhancing immunization, to immunologic hypofunction model caused by large dosage hydrogenation cortisone and hydroxycarbamide
Immune function of mice has humidification.Barren wort total chromocor can significantly increase the specific immunity and non-specificity of both models
Thus immune function prompts the inhibiting effect that general flavone hydrogenates cortisone to the facilitation that nucleic acid synthesizes with antagonism large dosage to have
Substantial connection.Barren wort total chromocor has anti-inflammatory effect, anti-aging and antitumor action.China is point of Herba Epimedii medicinal plant
Cloth center, resources advantage is apparent, the reliable effect of the main component barren wort total chromocor of Herba Epimedii, has development prospect.
Radix Astragali is the root of leguminous herbaceous plant's astragalus mongolicus, Astragalus membranacus, and pharmacological property is sweet and tepor, returns spleen, lung channel have
Strengthening spleen and middle warmer, yang invigorating, invigorating qi for consolidating superficies, inducing diuresis for removing edema, pus draining and toxin expelling, myogenic and other effects.Modern pharmacological research confirms Radix Astragali
There are anti peroxidation of lipid, antioxidant radical, and the formation of liver fibrosis has the participation of various kinds of cell factor, with oxygen freedom
Base, lipid peroxidation product etc. are closely related.Radix Astragali primary pharmacological activity ingredient is saponins compound Astragaloside IV, through pharmacology
Research shows that Astragaloside IV has immunological regulation, Organoprotective, adjusts various pharmacology such as Apoptosis, anti-inflammatory, antiviral
Effect.
Contain flavonols and its glycoside, a variety of Flavonoid substances such as isoflavones and its glycoside, Recent study table in Radix Astragali
Bright Radix Astragali flavone constituents, which have to adjust, to be immunized, resists myocardial ischemia, liver protection, anti-inflammatory, anti-mutation, removing a variety of pharmacology such as free radical
Effect.Radix Astragali flavone substance acts on obviously the shield shield of hepatic injury, significantly improves hepatic injury haemorrhagic shock.Rabbit Reperfu- sion
Afterwards for 24 hours, plasma alanine aminopherase (ALT) and asparagus fern aminopherase (AST) value increase, and blood gas index changes
Become, can effectively inhibit the raising of ALT, AST caused by ischemia-reperfusion, the variation of blood gas index to be obviously improved, show Huang
Stilbene general flavone has protective action as a kind of antioxidant to hepatic injury caused by hemorrhagic shock-reperfusion.It was found that astragalus flavonid
The hepatic injury degree of gavage paracetamol (paracetamol) mouse can be made substantially reduced, and action intensity and drug concentration
Directly proportional, mechanism of action may be with the certain metabolic processes of inhibition paracetamol and reduction metabolism of acetaminophen mistake
It is related that toxic metabolite is generated in journey.
Extract of Radix Ginseng stem and leaf of the present invention plays enhancing and is immunized, anti-oxidant, promotion liver cell metabolism, anti-hepatic fibrosis, inhibition
The generation of liver tumour；Milk thistle and epimedium flavone have antioxidant radical, anti peroxidation of lipid, protection liver plasma membrane, resist
Fibrosis effect；Radix Astragali as Qi-tonifying drug by the research of modern pharmacology its shown in preventing liver fibrosis it is good
Curative effect.The preparation applied in this patent based on the above reasons be suitable for it is all suffer from hepatopathy crowd, especially more suitable for chronic
Hepatitis and the crowd for tending to liver fibrosis.
In a word the present invention is characterized in that containing to liver after four ginseng, milk thistle, Herba Epimedii, Radix Astragali active principle cooperations
Active ingredient ginsenoside with health role, what milk thistle, barren wort total chromocor and astragaloside formed has liver protection, resists
The prescription of the health medicine of fibrosis effect.The advantage of the invention is that extract of Radix Ginseng stem and leaf fictitious hosts are high in the present invention
Expensive ginseng, cooperation Herba Silybi mariani flavone, epimedium flavone, astragalus root total saponin more play raising and are immunized, and increase Liver protection wound and make
With and prevent the formation of liver fibrosis.It is suitable for various hepatitis, the health care of cirrhotic population and supplies treatment, the composition is edible
Convenient, healthy, safety.
To confirm that effectiveness of the invention, the present invention select case 100 to be counted for one, see the table below altogether.
Typical clinical example：
Poplar：48 years old, man.Mild Obesity, with diabetes B, moderate fatty liver, hypoimmunity.After this product 3 months,
Blood glucose target is substantially reduced, and evening total urination time is switched to 2-3 times by original 4-5 times, and sleep quality increases, and feels energy
The vigorous steady and sure sleep of energy, hunger are also declined.It after 6 months, is checked to hospital, transaminase index decreased, moderate
Fatty liver switchs to mild fatty liver.
Mr. Wang：62 years old, man.Suffer from chronic hepatitis B 3 years, often there is Nausea and vomiting, loss of appetite, tiredness sense, upper right
Abdomen pain, abnormal liver function, the severity that stair activity is all panted.This product is used in the original plan 6 months, after being finished 2 months, feel essence
Power is vigorous, and stair activity is not being panted as before, can suitably do some housework, and nausea and vomiting sense is decreased obviously, appetite
Good very much, liver function numerical value tends to be normal, is gradually become shallower as before urine color is opposite.There are many gather colour of entire people.
Mr. Wang：57 years old, man.Since chronic alcoholism is adopted with alcoholic liver because not paying attention to developing into hepatic sclerosis in turn
After taking treatment, the state of an illness could not be controlled, liver cancer is converted to.Entire people gradually becomes thin, and right abdomen pain is serious, and abdomen occurs in fever from head to foot
The symptoms such as swollen diarrhea jaundice.After taking this product three months.Pass through transmission electron microscope observing to human liver cancer cell BEL-7402, HepG2
There is the metamorphosis of apoptosis.To occur to inhibit tumour growth effect.Weak sense decrease is felt, appetite increases.Abdominal distension
The symptoms such as diarrhea jaundice are alleviated.
Lee：46 years old, man.Two years ago suffers from chronic hepatitis, and because autoimmunity is low, hepatitis is converted into liver fibrosis.Chang You
Sour regurgitation, belch, hiccup, upper abdomen pain and Abdeminal pain sense, bleeding gums are serious, and there are purple plague purpura and bleeding in skin and mucous membrane many places
Point.Abnormal liver function, laminin is horizontal significantly raised, and hyaluronidase extremely increases in serum, is particularly easy to catch a cold, entirely
People is faint.After this product 3 months, indices are declined than before, under sour regurgitation, vomiting, feeling of pain feeling of repletion are apparent
Drop, bleeding gums situation also weaken relatively, and flu is converted into interim flu throughout the year, and tiredness sense obviously weakens, upper to go downstairs
It is terraced easily many, it is full of vitality.
Specific implementation mode
Embodiment 1
A kind of composition for protecting liver and preventing liver fibrosis from being formed, by major ingredient extract of Radix Ginseng stem and leaf 20-40%, water
Fly Ji extract 20-35%, excessive sheep extract 10-20%, Astragalus Root P.E 10-20% and auxiliary material plant amylum 10-20% by weight
Composition.
Preparation method：1. providing raw material：Extract of Radix Ginseng stem and leaf is made as follows：After gen-seng haulms are dried, warp
Crushing is crossed, the water measured using 15 times is solvent, and three times with the extraction of 95% ethyl alcohol, extraction time is respectively 3h, 3h and 2h；Extracting solution plate
Frame filters, and merges extracting solution and stands 24 hours, take supernatant, when sodium hydroxide being added to adjust pH value to 10~12, through macroreticular resin
Absorption, is washed with water impurity elimination, and alcohol-water elutes general ginsenoside, concentrate eluant, decoloration, recycling ethyl alcohol to no alcohol；Continue to concentrate
Water is evaporated to proportion d=1.15；Spray drying, pulverizes and sieves.Milk thistle extract is made as follows：By milk thistle
Herb medicinal material 1kg coarse crushings, 8 times of amount hexanes reflux degreasings of grinding medicinal materials, are vaporized after hexane and measure 75% alcohol refluxs with 12 times
Extraction 3 times 2 hours every time, merges extracting solution, recycles ethyl alcohol, and milk thistle ethanol extract is concentrated under reduced pressure to obtain；Milk thistle ethyl alcohol
Extract adds 80 DEG C of hot water dissolvings, after cooling with hydrochloric acid tune PH to 2~3, stands 12 hours with, filtering, and filtrate is with 2.5 kg
HPD427 macroporous resin adsorptions are first rinsed with purified water to colourless, discard eluent, then with 2% 9 L of sodium bicarbonate solution
Elution, and add 16 L purified waters are continuous to wash, it collects alkali and water continues washing lotion；5% hydrochloric acid solution tune pH to 2~4 of eluent, with 6 L's
Polyamide adsorbs, then is eluted with 70% ethyl alcohol, 10 L, collects alcohol eluen, is concentrated under reduced pressure, and vacuum drying obtains concentration about
For 60% or so Herba Silybi mariani total flavones.Shorthorned Epimedium P.E is made as follows：Take epimedium herb, 15 times of bodies of dosing material
75% long-pending ethyl alcohol decocts refluxing extraction 3 hours, and filtration, filtrate is spare；The dregs of a decoction add 75% alcohol reflux 2 of 13 times of volumes small again
When, it filters, filtrate is spare；The dregs of a decoction add 75% alcohol reflux of 10 times of volumes to extract 2 hours again, filtration, and filtrate is spare；Merge 3
Secondary extracting solution is concentrated under reduced pressure into relative density d=1.10~1.20 (50~60 DEG C) concentrate, 2 times of volumes is added in concentrate
Water make dissolving, place about 4~6 hours, filtering, filtrate be first washed with water to washing lotion by D-101 types macroporous resin column be it is colourless,
Flow velocity is 45ml/ points, discards water elution, then with 95% ethyl alcohol (4~5 times of amount bed volumes) elution, collect eluent, recycling
Ethyl alcohol is simultaneously concentrated into relative density d=1.10~1.20 (50~60 DEG C), dry, pulverize, obtains barren wort total chromocor.Radix Astragali
Extract is made as follows：5 kg of Milkvetch Root be placed in extractor with 75% alcohol reflux extract 2 times, the 1st time plus
10 times of amount alcohol refluxs extract 3 hours, the 2nd time plus 8 times amount alcohol refluxs and extract 2 hours, merge extracting solution, second is recovered under reduced pressure
Alcohol is concentrated into 1:1；The hot water dissolving for adding double quantity, stands overnight, filtering；Filtrate D101 large pore resin absorption column upper props,
Filtrate adsorbed by macroporous absorbent resin after first with the distillation water elution of 4 times of column volumes, to remove carbohydrate content, after with 4 times of cylinders
70% long-pending ethanol elution, collects 70% ethanol eluate, and decompression is concentrated to give astragalus root total saponin.
2. weighing extract of Radix Ginseng stem and leaf that is dry, crushing, milk thistle extract, Shorthorned Epimedium P.E, Radix Astragali according to quantity to carry
Object is taken, is placed in blender with plant amylum, is stirred evenly；
3. the material of mixing is packed by dosage form, dosage form is the various solid pharmaceutical preparations such as granule, capsule, tablet or powder.
Embodiment 2
A kind of composition for protecting liver and preventing liver fibrosis from being formed, by major ingredient extract of Radix Ginseng stem and leaf 25%, fine grinding
Ji extract 25%, excessive sheep extract 20%, Astragalus Root P.E 15% and auxiliary material plant amylum 15% form by weight.
The preparation method is the same as that of Example 1.
Embodiment 3
It is a kind of to be used to protect liver and prevent the composition that liver fibrosis is formed with capsule formula（100g）, gen-seng haulms is taken to carry
Take object 25g, milk thistle extract（Herba Silybi mariani flavone）25g, Shorthorned Epimedium P.E（Epimedium flavone）20g, Astragalus Root P.E
15g, plant amylum 15g, it is 12 hours dry in 50 DEG C of environment, particle is made, drying is crushed with pulverizer, crosses 120 mesh sieve, dress
Capsule.Or take each parts by weight 12 hours dry in 50 DEG C of environment, particle is made, then prepares piece agent.
Embodiment 4
Take extract of Radix Ginseng stem and leaf 35g, milk thistle extract（Herba Silybi mariani flavone）25g, Shorthorned Epimedium P.E（Epimedium flavone）
15g, Astragalus Root P.E 10g, plant amylum 15g is 12 hours dry in 50 DEG C of environment, and particle is made, dry to use pulverizer powder
It is broken, 120 mesh sieve is crossed, it is encapsulated.Or take each parts by weight 12 hours dry in 50 DEG C of environment, particle is made, then prepares in flakes
Agent.
Embodiment 5
Take extract of Radix Ginseng stem and leaf 20g, milk thistle extract（Herba Silybi mariani flavone）35g, Shorthorned Epimedium P.E（Epimedium flavone）
15g, Astragalus Root P.E 15g, plant amylum 15g is 12 hours dry in 50 DEG C of environment, and particle is made, dry to use pulverizer powder
It is broken, 120 mesh sieve is crossed, it is encapsulated.Or take each parts by weight 12 hours dry in 50 DEG C of environment, particle is made, then prepares in flakes
Agent.
Claims (8)
1. a kind of composition for protecting liver and preventing liver fibrosis from being formed contains group by following raw materials percentage by weight
At：
The excessive sheep extract 10-20% of extract of Radix Ginseng stem and leaf 20-40% milk thistle extracts 20-35%
Astragalus Root P.E 10-20% plant amylums 10-20%.
2. a kind of composition for protecting liver and preventing liver fibrosis from being formed according to claim 1, feature exist
It is formed in being contained by following raw materials percentage by weight：
25% milk thistle extract of extract of Radix Ginseng stem and leaf, 25% Shorthorned Epimedium P.E 20%
15% plant amylum 15% of Astragalus Root P.E.
3. a kind of composition for protecting liver and preventing liver fibrosis from being formed according to claim 1, feature exist
It is made as follows in the extract of Radix Ginseng stem and leaf：After gen-seng haulms are dried, by crushing, the water measured with 15 times is
Solvent, three times with the extraction of 95% ethyl alcohol, extraction time is respectively 3h, 3h and 2h；Extracting solution plate-frame filtering merges extracting solution and stands
24 hours, supernatant is taken, when sodium hydroxide being added to adjust pH value to 10~12, through macroporous resin adsorption, impurity elimination, ethyl alcohol-is washed with water
Water elution general ginsenoside, concentrate eluant, decoloration, recycling ethyl alcohol to no alcohol；Continue concentration and evaporation water to proportion d=1.15；Spray
Mist dry, pulverize sieving.
4. a kind of composition for protecting liver and preventing liver fibrosis from being formed according to claim 1, feature exist
It is made as follows in the milk thistle extract：Milk thistle herb medicinal material coarse powder is broken, 8 times of amount hexanes of grinding medicinal materials
Flow back degreasing, is extracted 3 times with 12 times of 75% alcohol refluxs of amount after being vaporized hexane, 2 hours every time, merges extracting solution, recycle ethyl alcohol,
And milk thistle ethanol extract is concentrated under reduced pressure to obtain；Milk thistle ethanol extract adds 80 DEG C of hot water dissolvings, after cooling with hydrochloric acid tune
PH to 2~3 stands 12 hours so that filtering, 2.5 kg HPD427 macroporous resin adsorptions of filtrate are first rinsed with purified water to nothing
Color, discards eluent, then with 2% sodium bicarbonate solution 9 L elutions, and adds 16 L purified waters are continuous to wash, and collects alkali and water is continuous washes
Liquid；5% hydrochloric acid solution tune pH to 2~4 of eluent is adsorbed with the polyamide of 6 L, then is eluted with 70% ethyl alcohol, 10 L, is received
Collect alcohol eluen, be concentrated under reduced pressure, vacuum drying obtains the Herba Silybi mariani total flavones that concentration is about 60% or so.
5. a kind of composition for protecting liver and preventing liver fibrosis from being formed according to claim 1, feature exist
It is made as follows in the Shorthorned Epimedium P.E：Epimedium herb, 75% ethyl alcohol of 15 times of volumes of dosing material is taken to decoct back
Stream extraction 3 hours, filtration, filtrate are spare；The dregs of a decoction add 75% alcohol reflux 2 hours of 13 times of volumes again, filtration, and filtrate is spare；
The dregs of a decoction add 75% alcohol reflux of 10 times of volumes to extract 2 hours again, filtration, and filtrate is spare；Merge No. 3 extracting solutions, is concentrated under reduced pressure
To relative density d=1.10~1.20 (50~60 DEG C) concentrate, the water of 2 times of volumes is added in concentrate makes dissolving, places about 4
~6 hours, filtering, it was colourless that filtrate, which is first washed with water by D-101 types macroporous resin column to washing lotion, and flow velocity is 45ml/ points, is discarded
Water elution, then with 95% ethyl alcohol (4~5 times of amount bed volumes) elution, collect eluent, recycle ethyl alcohol and be concentrated into relatively close
It spends d=1.10~1.20 (50~60 DEG C), dry, pulverize, obtain barren wort total chromocor.
6. a kind of composition for protecting liver and preventing liver fibrosis from being formed according to claim 1, feature exist
It is made as follows in the Astragalus Root P.E：Milkvetch Root is placed in extractor to be extracted 2 times with 75% alcohol reflux,
1st time plus 10 times amount alcohol reflux extracts 3 hours, the 2nd time plus 8 times amount alcohol refluxs and extracts 2 hours, merges extracting solution, decompression
Ethyl alcohol is recycled, is concentrated into 1:1；The hot water dissolving for adding double quantity, stands overnight, filtering；Filtrate D101 large pore resin absorption columns
Upper prop, filtrate adsorbed by macroporous absorbent resin after first with the distillation water elution of 4 times of column volumes, to remove carbohydrate content, after with 4 times
70% ethanol elution of column volume, collects 70% ethanol eluate, and decompression is concentrated to give astragalus root total saponin.
7. a kind of preparation method of the composition as described in claim 1 for protecting liver and preventing liver fibrosis from being formed,
Include the following steps：
1. extract of Radix Ginseng stem and leaf that is dry, crushing, milk thistle extract, Shorthorned Epimedium P.E, Astragalus Root P.E are weighed according to quantity,
It is placed in blender, stirs evenly with plant amylum；
2. the material of mixing is packed by dosage form.
8. a kind of preparation side of composition for protecting liver and preventing liver fibrosis from being formed according to claim 7
Method, it is characterised in that the combination dosage form is granule, capsule, tablet or powder.
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| CN102579561A (en) * | 2012-03-16 | 2012-07-18 | 甘肃中医学院 | Method for extracting total flavones and total saponins from astragalus |
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| CN102579561A (en) * | 2012-03-16 | 2012-07-18 | 甘肃中医学院 | Method for extracting total flavones and total saponins from astragalus |
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