CN108414638A - The detection method of active constituent in a kind of quick detection common yam rhizome powder - Google Patents
The detection method of active constituent in a kind of quick detection common yam rhizome powder Download PDFInfo
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- CN108414638A CN108414638A CN201810248718.0A CN201810248718A CN108414638A CN 108414638 A CN108414638 A CN 108414638A CN 201810248718 A CN201810248718 A CN 201810248718A CN 108414638 A CN108414638 A CN 108414638A
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- yam rhizome
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- rhizome powder
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- 238000001514 detection method Methods 0.000 title claims abstract description 60
- 239000000843 powder Substances 0.000 title claims abstract description 46
- 239000000470 constituent Substances 0.000 title claims abstract description 20
- 244000281702 Dioscorea villosa Species 0.000 title claims abstract 12
- 238000001035 drying Methods 0.000 claims abstract description 16
- 238000005119 centrifugation Methods 0.000 claims abstract description 11
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 239000006228 supernatant Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 claims description 24
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 18
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 claims description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 229960000458 allantoin Drugs 0.000 claims description 12
- 229930183633 Batatasin Natural products 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- CTENFNNZBMHDDG-UHFFFAOYSA-N Dopamine hydrochloride Chemical compound Cl.NCCC1=CC=C(O)C(O)=C1 CTENFNNZBMHDDG-UHFFFAOYSA-N 0.000 claims description 8
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 claims description 8
- 229960001231 choline Drugs 0.000 claims description 8
- 229960001149 dopamine hydrochloride Drugs 0.000 claims description 8
- 239000007853 buffer solution Substances 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 238000002604 ultrasonography Methods 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 4
- 235000019441 ethanol Nutrition 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 238000000825 ultraviolet detection Methods 0.000 claims description 3
- QPFMBZIOSGYJDE-UHFFFAOYSA-N 1,1,2,2-tetrachloroethane Chemical class ClC(Cl)C(Cl)Cl QPFMBZIOSGYJDE-UHFFFAOYSA-N 0.000 claims description 2
- 239000008363 phosphate buffer Substances 0.000 claims description 2
- 238000007689 inspection Methods 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 abstract description 5
- 238000005251 capillar electrophoresis Methods 0.000 abstract description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract description 5
- 239000010452 phosphate Substances 0.000 abstract description 5
- 238000002137 ultrasound extraction Methods 0.000 abstract 1
- 240000005717 Dioscorea alata Species 0.000 description 26
- 235000004879 dioscorea Nutrition 0.000 description 26
- 238000000034 method Methods 0.000 description 7
- 239000012071 phase Substances 0.000 description 7
- 235000002722 Dioscorea batatas Nutrition 0.000 description 4
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 4
- 240000001811 Dioscorea oppositifolia Species 0.000 description 4
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000004853 microextraction Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 241000234272 Dioscoreaceae Species 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000218989 Trichosanthes Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 210000001643 allantois Anatomy 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000008472 epithelial growth Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- -1 hydrochloric acid DOPA Amine Chemical class 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 229960004502 levodopa Drugs 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000005426 pharmaceutical component Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- DHRLEVQXOMLTIM-UHFFFAOYSA-N phosphoric acid;trioxomolybdenum Chemical compound O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.OP(O)(O)=O DHRLEVQXOMLTIM-UHFFFAOYSA-N 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of detection methods of active constituent in quick detection common yam rhizome powder.The present invention centrifuges after utilizing ethanol water ultrasonic extraction after first drying common yam rhizome powder, then the supernatant 2ml after centrifugation is taken to be mixed with 200ul extractants, mixture is quickly squeezed into 8ml water using syringe, it is vortexed, after centrifugation, with after the dissolving of 100ul 30mmol/L phosphate after lower phase is dried, finally analyzed it with Capillary Electrophoresis UV detector.The detection method of the present invention is quick, sensitive, safely and effectively, can be widely used in the detection of various common yam rhizome powders.
Description
Technical field
The present invention relates to field of food detection, the detection method of active constituent in specially a kind of quick detection common yam rhizome powder.
Background technology
Chinese yam is Dioscoreaceae plant, original name Chinese yam, also referred to as Chinese yam, and pharmacological research shows that common yam rhizome powder is a large amount of in addition to containing
Protein, various vitamins, except trace element and carbohydrate, also contain more pharmaceutical components, such as mucopolysaccharide, allantois
Element, batatasins, choline, Dopamine hydrochloride etc. are the very high integration of drinking and medicinal herbs food of nutritive value.Common yam rhizome powder have invigorating the spleen, tonifying lung,
It reinforces the kidney, strengthening the essence and other effects.Research shows that the allantoin in common yam rhizome powder has anti-irritant object, analgesia, promotes epithelial growth, disappears
The effects that scorching antibacterial.Occur adulterant common yam rhizome powder currently on the market, is often consumed with deceptions such as cassava, root of Chinese trichosanthes, sweet potato, ginseng potatos
Person.Therefore, the detection method for developing allantoin in common yam rhizome powder has great importance.
Content of Allantoin analysis method mainly has pH methods, ultraviolet spectrophotometry, thin layer chromatography scanning, high-efficient liquid phase color at present
Spectrometry, but that there are sensitivity is not high for these methods, detects the not congruent deficiency of sample.In version in 2015《Chinese Pharmacopoeia》It is defined
The detection method of mountain the effective elements of the medicine is：It takes this product powder 5g dichloromethane 30ml to flow back 2 hours to filter, after filtrate drying,
Residue makes dissolving with 1ml dichloromethane, is tested according to 0502 thin-layered chromatography of general rule, pipette samples point in silica gel-G lamellaes, with
Acetate-methanol-strong ammonia solution ratio is 9: 1:0.5 is solvent, is unfolded, and is taken out, and 10% phosphomolybdic acid ethanol of spray is dried,
It is clear to point colour developing at 105 °C.In test sample chromatography, on the position of control medicinal material chromatography phase, the point of same color is shown.It should
Method not only used a large amount of organic solvent and quantitative inaccuracy.Therefore it must be set up a kind of quick, simple, science mirror
Determine method, the link that goods is entered to high-volume common yam rhizome powder carries out effective monitoring based on this, and this law provides a kind of quickly detection Chinese yam
The detection method of active constituent in powder.
Invention content
It is not high that there are sensitivity when in order to solve common yam rhizome powder detection, detects the not congruent problem of sample, and the present invention discloses a kind of quick
Detect the detection method of active constituent in common yam rhizome powder.
The method of the present invention includes the following steps：
（1）By common yam rhizome powder under the conditions of 40-60 DEG C after dry 4-6 hour, accurately weighing 1.0g sample powders and 8-10ml
After ethanol-water solution vortex 20-40s, ultrasound 30-45min postcoolings, will mix in 400-600W power, 40-60 DEG C of water-bath
Liquid centrifuges 5-10min under the conditions of 4000-6000rpm；
（2）It takes the supernatant 2ml after centrifugation to be mixed with 200ul extractants, is quickly squeezed into mixture in 8ml water using syringe,
It is dry after lower phase is taken out after centrifuging 3-5min under the conditions of 4000-6000rpm after vortex 20-40s；
（3）After sample after drying is dissolved with 100ul 30mmol/L phosphate buffers, with Capillary Electrophoresis-ultraviolet detection
Device analyzes it.
Further, step（1）Described in active constituent include allantoin, batatasins, choline, Dopamine hydrochloride
Further, step（1）Described in ethanol-water solution in ethyl alcohol volume content be 60-70%.
Further, step（2）Described in extractant be dichloromethane, chloroform, in 1,1,2,2- tetrachloroethanes
One kind.
Further, step（2）Described in lower phase volume be 180-200ul；The drying mode is that nitrogen drying is dry.
Further, step（3）Described in buffer solution pH value be 9.15-9.35.
Further, step（3）Described in Capillary Electrophoresis-ultraviolet detection condition be to apply voltage 20-25kV；Detection
Wavelength is 200nm；Sampling condition is 30mbar pressure injections 10 seconds, and detection temperature is 25 DEG C.
The detection method of active constituent has in a kind of quick detection common yam rhizome powder of the present invention compared with prior art
Following advantageous effect, the present invention use EtOH Sonicate auxiliary extraction, and the solvent safety used is nontoxic and ultrasonic technique can be significant
Shorten extraction time.The highly polar impurity in part can not only be removed using dispersion liquid micro-extraction skill, reduce interference, reduce organic solvent
Dosage, while can significantly improve using the concentration effect of micro-extraction technique the sensitivity of detection；It is ultraviolet using Capillary Electrophoresis-
Separation detection technique can be realized is carried out at the same time detection to four kinds of active ingredients.The detection method of the present invention is quick, sensitive, safety
Effectively, can be widely used in the detection of various common yam rhizome powders.
Description of the drawings
Fig. 1 is the Capillary Electrophoresis chromatogram of allantoin, batatasins, choline, Dopamine hydrochloride standard sample, wherein hydrochloric acid DOPA
Amine, four kinds of allantoin, batatasins, choline material concentrations are 2ug/ml, and chromatogram ordinate indicates that peak intensity unit is uV.
Fig. 2 is the Capillary Electrophoresis chromatogram that the detection method extraction common yam rhizome powder of the present invention obtains, chromatogram ordinate table
Show that peak intensity unit is uV.
Wherein, peak 1 indicates that Dopamine hydrochloride, peak 2 indicate that allantoin, peak 3 indicate that allantoin, peak 4 indicate batatasins.
Specific implementation mode
【Embodiment 1】
The detection method of active constituent, includes the following steps in a kind of quick detection common yam rhizome powder：By common yam rhizome powder under the conditions of 50 DEG C
After dry 5 hours, after the 70% ethanol-water solution vortex 25s for accurately weighing 1.0g sample powders and 8ml, in 500W power,
Mixed liquor is centrifuged 6min by ultrasound 35min postcoolings in 50 DEG C of water-baths under the conditions of 5000rpm；Take centrifugation after supernatant 2ml with
After the mixing of 200ul dichloromethane, mixture is quickly squeezed into 8ml water using syringe, in 5000rpm conditions after vortex 25s
After lower centrifugation 4min, nitrogen drying is dry after lower phase is taken out；By the sample after drying with 100ul 30mmol/L phosphate (pH=
9.30) after buffer solution, applying voltage 20-25kV with Capillary Electrophoresis-UV detector；Detection wavelength is 200nm；Into
Batten part is 30mbar pressure injections 10 seconds, and detection temperature is detected under the conditions of being 25 DEG C.
【Embodiment 2】
The detection method of active constituent, includes the following steps in a kind of quick detection common yam rhizome powder：By common yam rhizome powder under the conditions of 60 DEG C
After dry 4 hours, after the 65% ethanol-water solution vortex 25s for accurately weighing 1.0 sample powders and 10ml, in 600W power,
Mixed liquor is centrifuged 7min by ultrasound 30min postcoolings in 50 DEG C of water-baths under the conditions of 5000rpm；Take centrifugation after supernatant 2ml with
After the mixing of 200ul chloroforms, mixture is quickly squeezed into 8ml water using syringe, in 6000rpm conditions after vortex 20s
After lower centrifugation 3min, nitrogen drying is dry after lower phase is taken out；By the sample after drying with 100ul 30mmol/L phosphate (pH=
9.15) after buffer solution, applying voltage 20-25kV with Capillary Electrophoresis-UV detector；Detection wavelength is 200nm；Into
Batten part is 30mbar pressure injections 10 seconds, and detection temperature is detected under the conditions of being 25 DEG C.
【Embodiment 3】
The detection method of active constituent, includes the following steps in a kind of quick detection common yam rhizome powder：By common yam rhizome powder under the conditions of 60 DEG C
After dry 5 hours, after the 60% ethanol-water solution vortex 35s for accurately weighing 1.0g sample powders and 10ml, in 600W power,
Mixed liquor is centrifuged 5min by ultrasound 30min postcoolings in 55 DEG C of water-baths under the conditions of 6000rpm；Take centrifugation after supernatant 2ml with
200ul1,1,2,2- tetrachloroethanes mixing after, mixture is quickly squeezed into 8ml water using syringe, after vortex 25s
After centrifuging 4min under the conditions of 5000rpm, nitrogen drying is dry after lower phase is taken out；By the 100ul 30mmol/L of the sample after drying
After phosphate (pH=9.30) buffer solution, applying voltage 20-25kV with Capillary Electrophoresis-UV detector；Detection wavelength
For 200nm；Sampling condition is 30mbar pressure injections 10 seconds, and detection temperature is detected under the conditions of being 25 DEG C.
【Embodiment 4】
The detection method of active constituent, includes the following steps in a kind of quick detection common yam rhizome powder：By common yam rhizome powder under the conditions of 60 DEG C
After dry 4 hours, after the 65% ethanol-water solution vortex 30s for accurately weighing 1.0g sample powders and 9ml, in 550W power,
Mixed liquor is centrifuged 6min by ultrasound 45min postcoolings in 55 DEG C of water-baths under the conditions of 6000rpm；Take centrifugation after supernatant 2ml with
After the mixing of 200ul dichloromethane, mixture is quickly squeezed into 8ml water using syringe, in 6000rpm conditions after vortex 25s
After lower centrifugation 3min, nitrogen drying is dry after lower phase is taken out；By the sample after drying with 100ul 30mmol/L phosphate (pH=
9.35) after buffer solution, applying voltage 20-25kV with Capillary Electrophoresis-UV detector；Detection wavelength is 200nm；Into
Batten part is 30mbar pressure injections 10 seconds, and detection temperature is detected under the conditions of being 25 DEG C.
For example, Fig. 1 is the Capillary Electrophoresis chromatogram of allantoin, batatasins, choline, Dopamine hydrochloride standard sample, Fig. 2
It is the Capillary Electrophoresis chromatogram that the detection method extraction common yam rhizome powder of the present invention obtains.It can be seen from the figure that the present invention can be from
Detect allantoin, batatasins, choline, Dopamine hydrochloride in common yam rhizome powder, and the matrix of common yam rhizome powder to detect these four activity at
Divide without influence.
The allantoin that is detected in common yam rhizome powder in embodiment 1-4, batatasins, choline, Dopamine hydrochloride concentration such as 1 institute of table
Show.
For the ordinary skill in the art, specific embodiment is only exemplarily described the present invention,
Obviously the present invention specific implementation is not subject to the restrictions described above, as long as use the inventive concept and technical scheme of the present invention into
The improvement of capable various unsubstantialities, or it is not improved by the present invention design and technical solution directly apply to other occasions
, within protection scope of the present invention.
Claims (7)
1. the detection method of active constituent in a kind of quick detection common yam rhizome powder, it is characterised in that include the following steps：
（1）By common yam rhizome powder under the conditions of 40-60 DEG C after dry 4-6 hour, accurately weighing 1.0g sample powders and 8-10ml
After ethanol-water solution vortex 20-40s, ultrasound 30-45min postcoolings, will mix in 400-600W power, 40-60 DEG C of water-bath
Liquid centrifuges 5-10min under the conditions of 4000-6000rpm；
（2）It takes the supernatant 2ml after centrifugation to be mixed with 200ul extractants, is quickly squeezed into mixture in 8ml water using syringe,
It is dry after lower phase is taken out after centrifuging 3-5min under the conditions of 4000-6000rpm after vortex 20-40s；
（3）After sample after drying is dissolved with 100ul 30mmol/L phosphate buffers, with the ultraviolet inspection of Capillary Electrophoresis-
Device is surveyed to analyze it.
2. the detection method of active constituent in a kind of quick detection common yam rhizome powder as described in claim 1, it is characterised in that step
（1）Described in active constituent include allantoin, batatasins, choline, Dopamine hydrochloride.
3. the detection method of active constituent in a kind of quick detection common yam rhizome powder as described in claim 1, it is characterised in that step
（1）Described in ethanol-water solution in ethyl alcohol volume content be 60-70%.
4. the detection method of active constituent in a kind of quick detection common yam rhizome powder as described in claim 1, it is characterised in that step
（2）Described in extractant be dichloromethane, chloroform, one kind in 1,1,2,2- tetrachloroethanes.
5. the detection method of active constituent in a kind of quick detection common yam rhizome powder as described in claim 1, which is characterized in that step
（2）Described in lower phase volume be 180-200ul；The drying mode is that nitrogen drying is dry.
6. the detection method of active constituent in a kind of quick detection common yam rhizome powder as described in claim 1, which is characterized in that it is special
Sign is step（3）Described in buffer solution pH value be 9.15-9.35.
7. the detection method of active constituent in a kind of quick detection common yam rhizome powder as described in claim 1, it is characterised in that step
（3）Described in Capillary Electrophoresis-ultraviolet detection condition be apply voltage 20-25kV, Detection wavelength 200nm；Sampling condition
For 30mbar pressure injections 10 seconds, detection temperature was 25 DEG C.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06160366A (en) * | 1992-10-27 | 1994-06-07 | Jasco Corp | Measuring method and device for catecholamine in biological fluid |
| CN101231274A (en) * | 2008-01-28 | 2008-07-30 | 河南中医学院 | Method for rapid measuring allantoin content in yam using near infrared spectrum |
| CN104330516A (en) * | 2014-11-26 | 2015-02-04 | 广西壮族自治区药用植物园 | Method for simultaneously determining content of allantoin and adenosine in Chinese yams |
-
2018
- 2018-03-24 CN CN201810248718.0A patent/CN108414638A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06160366A (en) * | 1992-10-27 | 1994-06-07 | Jasco Corp | Measuring method and device for catecholamine in biological fluid |
| CN101231274A (en) * | 2008-01-28 | 2008-07-30 | 河南中医学院 | Method for rapid measuring allantoin content in yam using near infrared spectrum |
| CN104330516A (en) * | 2014-11-26 | 2015-02-04 | 广西壮族自治区药用植物园 | Method for simultaneously determining content of allantoin and adenosine in Chinese yams |
Non-Patent Citations (4)
| Title |
|---|
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