CN108404212A - A kind of preparation method of acellular dermal matrix material - Google Patents
A kind of preparation method of acellular dermal matrix material Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3633—Extracellular matrix [ECM]
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
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Abstract
" a kind of preparation method of acellular dermal matrix material " of the invention, belongs to biomaterial preparing technical field.Shown preparation method includes the following steps：(1) skin histology of human or animal is placed in fixative and processing is fixed；(2) skin histology after fixing process is placed in alkaline solution and carries out de- cell processing；(3) by de- cell, treated that skin histology carries out extraction cleaning treatment；The fixative is glutaraldehyde, formaldehyde or Peracetic acid；The alkaline solution is selected from NaOH or KOH solution of the mass percent concentration range between 4% 15%；The cleaning solution used in the extraction cleaning treatment is physiological saline.The gap of acellular dermal matrix material being prepared is handled through preparation method of the present invention and can reach 15 μm 42 μm, and voidage enables cell when products application to grow between 70% 90%, and is unlikely to cause Fiber Digestion to occur collapsing and void layer.
Description
Technical field
The present invention relates to biomaterial preparing technical fields, and in particular to a kind of preparation side of acellular dermal matrix material
Method.
Background technology
The ingredient of extracellular matrix is usually guarded between species, and can be resistant to by heterologous receptor.It is obtained from different tissues
Extracellular matrix, including skin, heart valve, blood vessel, nerve, skeletal muscle, tendon, ligament, submucous layer of small intestine, in group
Extensive research has been done in weaver's journey and the application of recyclability material.Cell free target is effectively to remove all cells and karyon
Substance, while minimizing to material bioactivity, the adverse effect of mechanical integrity.It is existing that there is also some for this field at present
Technology, such as：
Application for a patent for invention 201710682043.6 provides a kind of preparation method of acellular dermal matrix comprising with
Lower step：After malicious split-thickness skin graft go out with degreasing agent degreasing, cell is taken off through de-cell liquid, obtains product A；The product A is with olive
After bitter glycosides solution crosslinking, successively through alcohol rinsing, PBS solution rinsing, dry, irradiation sterilization, product B is obtained；The product B with
It after carboxymethyl chitosan solution processing, is rinsed through water, obtains product C；After the product C is impregnated with glutaraldehyde solution, through softening,
Moisturizing processing, again with physiological saline rinsing, after inactivation of virus, be lyophilized and acellular dermal matrix be made；The degreasing agent includes water
With following mass fraction：Soda ash 1.0%~4.0%；Caustic soda 1.0%~4.0%；Surfactant 0.1%~1.0%；Institute
It states surfactant and is selected from peregal, sodium dodecyl aminopropionitrile, fatty alcohol polyoxyethylene ether or Qula ketone；The de- cell
Liquid includes water and following mass fraction：Pancreatin 0.1%~0.5%；Activator 0.1%~0.3%；Degreasing agent 0.2%~
0.6%；The activator is ammonium sulfate；The degreasing agent is peregal or sodium dodecyl sulfate solution.
Application for a patent for invention 201710323525.2 discloses a kind of preparation method of Xenogenic acellular dermal matrix, special
Sign is, includes the following steps：(1) it is that skin source is taken to taking dermatotome to be lost hair or feathers, sterilized with dermatome to select BaMa miniature pig skin
Lower spready hide skin, sterile saline cleaning, skin rouses the anti-split-thickness skin graft for taking into 0.25~0.5mm, using laser drilling
Drilling is carried out to split-thickness skin graft, it is spare；(2) split-thickness skin graft made from step (1) is soaked in the adjacent benzene of 0.5~1.0% (m/v)
In base aqueous sodium phenolate solution, 6~8min is handled, sterile saline is cleaned 3~4 times, and 1~2% (m/v) three hydroxyl first is then added
Base methylamino ethanesulfonic acid aqueous solution, ultrasonic vibration handle 6~12h, a solution are replaced per 2h, wherein the trihydroxy methyl first being added
The weight ratio of amido ethanesulfonic acid aqueous solution and split-thickness skin graft is 10:1, obtain the corium of epidermis；(3) step (2) is obtained
Go the corium of epidermis to be placed in 0.2~0.4%TritonX-100 (v/v) solution, at room temperature oscillation washing 6~12h, with containing
4000U/L gentamicin normal saline solutions wash 3 times, are added and take off cell treatment fluid, act on 12 under the conditions of 37 DEG C~for 24 hours,
The weight ratio of the de- cell treatment fluid and the corium for removing epidermis is 10:1, it is water-soluble with the physiology salt of gentamicin containing 4000U/L
Liquid washs 3 times, then is cleaned 3~4 times with sterile saline, obtains acellular dermal；(4) the de- cell for obtaining step (3)
Corium is put into rotary drum, then by several times be added temperature be 35 DEG C, the carboxymethyl chitosan solution that pH is 5.7, react 30min after adjust
PH value is saved to 5.0, then reacts 75min, adjusts pH value to 6.8, puts net waste liquid after being disposed, then will treated material nothing
Bacterium physiology salt is washed 2 times, 15 minutes every time, is drained water after washing, is obtained acellular dermal matrix；(5) it will be handled through step (4)
To acellular dermal matrix be soaked in volumetric concentration be 0.1~0.3% (v/v) glutaraldehyde solution in be crosslinked 15~30min,
Sterile saline rinses 10~15min, is subsequently placed in 30~60min of immersion in softening agent solution, moisturizing is placed into after cleaning
In agent solution, 37 DEG C of 30~60min of isothermal holding, finally with the abundant wash and remove residual of sterile saline；It (6) will be through step
(5) acellular dermal matrix that processing obtains is put into the freezing chamber of freeze drier after freeze-drying, shaping packaging, then through gamma-rays
Radiation sterilization, as finished product.
Application for a patent for invention 201710247775.2 discloses a kind of preparation method of medical acellular dermal matrix, special
Sign is, including following operating procedure：1) it by after fresh animal skin fleshing, carries out disinfection, clean successively, hypertonic salt treatment,
It tears epidermis off, obtains pending corium skin graft；2) mixing that corium skin graft made from step 1) is put into lye and hydrogen peroxide is molten
It is impregnated 3~24 hours in liquid；3) treated that corium skin graft cut by step 2), cut open layer after be immersed in containing trypsase
Solution in, at a temperature of 35~40 DEG C, pH7~9, impregnate 1~12 hour；4) step 3) treated corium skin graft is cleaned
Afterwards, freezing storage, that is, complete.
But there are the following problems for above-mentioned existing method：The concentration of manufactured extracellular matrix, lye is high, the time of immersion
Long, treatment temperature is high, causes Fiber Digestion, occurs collapsing and void layer, this will cause cell when products application that cannot grow into, right
The use of finished product impacts.Its concentration of lye is low, soaking time is short, and treatment temperature is low, causes cell de- unclean, causes
The immune response of matrix causes product to use failure.
Invention content
Based on this field above shortcomings, the present invention is intended to provide one kind can make cell removing clean and protect
The integrality for demonstrate,proving dermal matrix fiber, to achieve the purpose that ensure product safety, high quality.
Technical scheme is as follows：
A kind of preparation method of acellular dermal matrix material, which is characterized in that include the following steps：
(1) skin histology of human or animal is placed in fixative and processing is fixed；
(2) skin histology after fixing process is placed in alkaline solution and carries out de- cell processing；
(3) by de- cell, treated that skin histology carries out extraction cleaning treatment；
The fixative is glutaraldehyde, formaldehyde or Peracetic acid；
The alkaline solution is selected from NaOH or KOH solution of the mass percent concentration range between 4%-15%；
The cleaning solution used in the extraction cleaning treatment is physiological saline.
The time of the fixing process is 2 hours or more.
The time of the de- cell processing is 15-30 hours, and temperature is 15-35 DEG C.
The time of the extraction cleaning treatment is 5-7 hours.
The preparation method further includes：After carrying out the extraction cleaning treatment, can change washing solution, continue cleaning until
Skin histology is translucent state.
The preparation method further includes：Cleaned skin histology will be extracted to be placed in amino acid solution.
The amino acid solution includes asparatate, glutamic acid or glycine；Preferably, the amino acid solution is dense
Degree is 0.5%-2%.
The acellular dermal matrix material preservation is in amino acid solution, and it is 7.4 or so to adjust solution ph.
The skin histology of the human or animal is the skin histology for eliminating fat, epidermis and hair.
The dermal matrix material that the preparation method is prepared.
In some specific embodiments of the invention, the preparation method carries out as follows：Take human or animal tissues
The skin of gained removes fat and fur, is put into fixative and fixes, fixative can be glutaraldehyde, formaldehyde or Peracetic acid.It should
Group, which is woven in fixer, at least to be retained 2 hours, or until the tissue is fixed.After tissue is fixed with suitable solution to its into
Row washing, which can be distilled water, and washing times can be multiple.
Then the tissue is placed in alkaline solution and carries out de- cell, alkaline solution can be NaOH or KOH solution.It places
Time is 15-30 hours, and temperature is 15-35 DEG C, concentration of lye 4%-15%.
The percent concentration being referred to herein is mass percent concentration.
After tissue takes off cell, extraction cleaning is carried out using physiological saline, soaking time is 5-7 hours, ensures the life of tissue
Object safety.It is found in practice, if without soaking and washing, the cytotoxicity of tissue is unqualified.
After organizing soaking and washing, washing solution is can change, is translucent until cleaning, tissue is then positioned over ammonia
Base acid Chinese medicine eliminates remaining free fixative and reduces pH.Amino acid can be asparatate, glutamic acid or glycine, dense
Degree is 0.5%-2%, and it is about 7.4 finally to adjust pH.
Present invention preserves intact extracellular matrix and active materials, for the growth of cell, differentiation, metabolism, tune
Section plays the role of not replaceable.By the tissue of the technical finesse, due to can be identified to host expresses group by host
The antigenic cellular material of active immunity is removed, and completely remains the form of acellular matrix, institutional framework, ultra microstructure are fixed
Position and tolerance etc. are all as former organized matrix.
The present invention relates to the methods that the skin histology obtained by human or animal tissues prepares biological cell epimatrix.This method can
Skin histology is handled by physicochemical manner, removal can cause the cell component repelled, obtain extracellular matrix frame, be used for
Transplanting or the surface of a wound for repairing life entity.
Host material generation is set to collapse in order to avoid the excessively high caused excessively digestion acellular matrix of concentration of lye, and alkali
Liquid concentration is too low and situation that de- cell effect can be caused undesirable occurs, the present invention through verify repeatedly find out it is a set of completely new
Dermal matrix takes off cellular processes, can using NaOH of the mass percent concentration range between 4%-15% or KOH solution
So that the voidage for the acellular dermal matrix material being finally prepared is maintained between 70%-90%, pore size 15 μm-
Between 42 μm, cell residue amount is 0, and the acellular dermal matrix material for having above-mentioned performance can perform well in dermatoplasty
And/or repair, while so that Skin Cell is quickly grown into, not generating immunogenicity, is safe to use, and will not be because of matrix fibre
Dimension excessively digests and generation collapses or void layer, ensure that the integrality and product quality of acellular dermal matrix material.
Description of the drawings
The acellular dermal matrix material that Fig. 1 is prepared by the preparation method that one embodiment of the invention provides at
Product figure.
The H- for the acellular dermal matrix material that Fig. 2 is prepared by the preparation method that one embodiment of the invention provides
E coloration result figures, × 400, as seen from the figure：After dermal matrix is handled by the above method, arrangement of collagen fibers is neat, fine and close,
Even dyeing, no oedema, disintegration.No cell structure is found.
The electricity for the acellular dermal matrix material that Fig. 3 is prepared by the preparation method that one embodiment of the invention provides
Mirror photo figure (scale：50 μm), it can be seen that after dermal matrix is handled by the above method, surface is smooth, locally there is a small amount of hole
Gap occurs, no any cell attachment.
The electricity for the acellular dermal matrix material that Fig. 4 is prepared by the preparation method that one embodiment of the invention provides
Mirror photo figure (scale：30 μm), it can be seen that：After dermal matrix is handled by the above method, surface is smooth, it is seen that a large amount of to interweave
The collagenous fibres (→) of arrangement, no any cell attachment.
Specific implementation mode
Present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments, but is not intended to limit the present invention
Range.Unless otherwise specified, the operation used in following embodiments is conventional method, and used material can be commercially available
It obtains.
The source of biomaterial
The skin histology of human or animal comes from：Human skin have drawn from no hyperlipidemia cardiovascular disease family history, serology inspection
Look into human immunodeficiency virus (exclusion of PCR methods), hepatitis B, hepatitis C virus, syphilis people each site tissue, source by
It contributes people or contributes relatives and agree to contribute tissue to hospital or be supplied to associated mechanisms to carry out clinical medicine use by hospital
Tissue.Animal skin, which has drawn from, pinpoints raising, procurement at designated enterprises, fixed point massacre, and carries out animal according to national relevant regulations
The animal skin of epidemic prevention, quarantine.
The present invention provides a kind of preparation method of acellular dermal matrix material.In all embodiments of the present invention, all
Has following common trait：The preparation method includes the following steps：
(1) skin histology of human or animal is placed in fixative and processing is fixed；
(2) skin histology after fixing process is placed in alkaline solution and carries out de- cell processing；
(3) by de- cell, treated that skin histology carries out extraction cleaning treatment；
The fixative is glutaraldehyde, formaldehyde or Peracetic acid；
It is molten in mass percent concentration NaOH between 4%-15% or KOH that the alkaline solution is selected from concentration range
Liquid；The cell for the acellular dermal matrix material that processing obtains can be made de- clean using this concentration of lye, not cause body
Immune response, while the acellular dermal matrix material for making processing obtain has enough gaps.It is handled through preparation method of the present invention
The gap for the acellular dermal matrix material being prepared can reach 15 μm -42 μm, and voidage makes production between 70%-90%
Cell is grown into when product are applied, and is unlikely to cause Fiber Digestion to occur collapsing and void layer.
The definition in " gap " herein：Additional space is vacated after cell clearance, in the space that original cell is vacateed i.e. gap.
Due to original biomaterial, such as corium, a series of processing such as de- cell are have passed through, take off the matrix frame structure meeting after cell
A degree of change occurs, therefore " gap " herein cannot be equal with the intercellular structural void in original corium.
The cleaning solution used in the extraction cleaning treatment is physiological saline.
There is " corium " word herein the conventional sense of this field, those skilled in the art can pass through routine techniques hand
Section obtains " corium " as described herein.
In a further embodiment, the time of the fixing process is 2 hours or more.The effect of this step is：It protects
Shield frame structure is not destroyed in de- cell processing procedure.
In some embodiments, the time of the de- cell processing is 15-30 hours, and temperature is 15-35 DEG C.Select these
Response parameter and the benefit of reaction condition are：Cell removing is clean, and frame keeps complete.
In further embodiments, the time of the extraction cleaning treatment is 5-7 hours.
In a further embodiment, the preparation method further includes：After carrying out the extraction cleaning treatment, changes and wash
Liquid is washed, continues to clean until skin histology is translucent state.Specifically, the cleaning solution is selected from purified water or distilled water.
In a still further embodiment, the preparation method further includes：Cleaned skin histology will be extracted to set
In amino acid solution.
In the particular embodiment, the amino acid solution includes asparatate, glutamic acid or glycine；This few class ammonia
Base acid is necessary to acellular dermal matrix frame components (based on collagen component) maintenance.
In a preferred embodiment, wherein a concentration of mass concentration 0.5%-2% of the amino acid solution.
In a more specific embodiment, the acellular dermal matrix material preservation is in amino acid solution, and adjusts molten
Liquid pH value is 7.4 or so；Using pH7.4 or so amino acid solution as acellular dermal matrix material preservation solution benefit
It is：For weakly alkaline solution, protein active, the living environment of simulated albumin can be kept.
There are many aspect of the preparation-obtained acellular dermal matrix material product application of preparation method of the present invention, skin wound
The reparation of face defect, such as burn and scald reparation, covering, guide tissue regeneration, such as mucous membrane of mouth reparation, hernia reparation, body wall defect
Reparation, ear nose larynx Mucous rehabilitation, corneal restoration etc.；Shaping application etc..
In certain embodiments, the skin histology of the human or animal is the skin group for eliminating fat, epidermis and hair
It knits, that is, dermis layer tissue.
The concrete operations of experimental example 1, the present invention
The skin obtained by human or animal tissues is taken, fat and fur are removed, is put into fixative and fixes, fixative can be penta
Dialdehyde, formaldehyde or Peracetic acid.The group, which is woven in fixer, at least to be retained 2 hours, or until the tissue is fixed.Tissue is solid
It is washed with suitable solution after fixed, which can be distilled water, and washing times can be multiple.
Then the tissue is placed in alkaline solution and carries out de- cell, alkaline solution can be NaOH or KOH solution.It places
Time is 15-30 hours, and temperature is 15-35 DEG C, concentration of lye 4%-15%.
After tissue takes off cell, extraction cleaning is carried out using physiological saline, soaking time is 5-7 hours, ensures the life of tissue
Object safety.It is found in practice, if without soaking and washing, the cytotoxicity of tissue is unqualified.
After organizing soaking and washing, washing solution is can change, is translucent until cleaning, tissue is then positioned over ammonia
Base acid Chinese medicine eliminates remaining free fixative and reduces pH.Amino acid can be asparatate, glutamic acid or glycine, dense
Degree is 0.5%-2%, and it is about 7.4 finally to adjust pH.
The impact of performance verification for the dermal matrix material that experimental example 2, method of the invention are prepared
The dermal matrix material that preparation method using the present invention obtains, through this field common detection methods, such as is invented
The detection method that patent application 201711056849.0 is recorded, detects following property indices, the results are as follows：
Cytotoxicity experiment result：As shown in Figures 2 and 3, cytotoxic；Immunogenic components are detected as 0；
De- cell effect data：Cell residue amount is 0；
Mechanical properties data：Tensile strength is more than 3MPa；Disruptive force is more than 20N；Bursting power is more than 35N；Suture power is more than
32N。
It is observed and is counted by Electronic Speculum, statistics obtains following gap data：As shown in Figure 3 and Figure 4, preparation method of the present invention
The voidage 70%-90% of obtained acellular dermal matrix material, pore size are 15 μm -42 μm.
Claims (10)
1. a kind of preparation method of acellular dermal matrix material, which is characterized in that include the following steps：
(1) skin histology of human or animal is placed in fixative and processing is fixed；
(2) skin histology after fixing process is placed in alkaline solution and carries out de- cell processing；
(3) by de- cell, treated that skin histology carries out extraction cleaning treatment；
The fixative is glutaraldehyde, formaldehyde or Peracetic acid；
The alkaline solution is selected from NaOH or KOH solution of the mass percent concentration range between 4%-15%；
The cleaning solution used in the extraction cleaning treatment is physiological saline.
2. preparation method according to claim 1, which is characterized in that the time of the fixing process is 2 hours or more.
3. preparation method according to claim 1, which is characterized in that the time of the de- cell processing is 15-30 hours,
Temperature is 15-35 DEG C.
4. requiring the preparation method described in 1 according to leaching, which is characterized in that the time of the extraction cleaning treatment is 5-7 hours.
5. preparation method according to claim 1 or 4, which is characterized in that further include：Carry out the extraction cleaning treatment
Afterwards, washing solution is can change, continues to clean until skin histology is translucent state.
6. according to any preparation methods of claim 1-5, which is characterized in that further include：It will extract cleaned
Skin histology is placed in amino acid solution.
7. preparation method according to claim 6, which is characterized in that the amino acid solution includes asparatate, paddy
Propylhomoserin or glycine；
Preferably, the mass percent concentration of the amino acid solution is 0.5%-2%.
8. according to a kind of preparation method of any acellular dermal matrix materials of claim 1-7, which is characterized in that institute
Acellular dermal matrix material preservation is stated in amino acid solution, and it is 7.4 or so to adjust solution ph.
9. preparation method according to claim 1, wherein the skin histology of the human or animal is to eliminate fat, table
The skin histology of skin and hair.
10. the dermal matrix material that any preparation methods of claim 1-9 are prepared.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109646718A (en) * | 2019-01-29 | 2019-04-19 | 北京颢美细胞基因生物技术有限公司 | Regenerating tissues base composition, preparation and application for micro-shaping |
| CN112190764A (en) * | 2020-09-17 | 2021-01-08 | 上海亚朋生物技术有限公司 | Allogeneic skin acellular method |
| CN114190075A (en) * | 2020-07-13 | 2022-03-15 | 爱恩斯生物科技(昆山)有限公司 | Hydrated acellular skin substitute material and preparation method thereof |
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