CN107029298A - A kind of medical acellular dermal matrix and preparation method thereof - Google Patents
A kind of medical acellular dermal matrix and preparation method thereof Download PDFInfo
- Publication number
- CN107029298A CN107029298A CN201710247775.2A CN201710247775A CN107029298A CN 107029298 A CN107029298 A CN 107029298A CN 201710247775 A CN201710247775 A CN 201710247775A CN 107029298 A CN107029298 A CN 107029298A
- Authority
- CN
- China
- Prior art keywords
- medical
- preparation
- concentration
- dermal matrix
- skin graft
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/362—Skin, e.g. dermal papillae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
Abstract
The present invention relates to medical biomaterial technical field, and in particular to a kind of medical acellular dermal matrix and preparation method thereof.Preparation method of the present invention includes after animal skin fleshing, successively carrying out disinfection, cleaning, after hypertonic salt treatment, is put into the mixed solution of alkali lye and hydrogen peroxide and soaks；Then it is immersed in again in trypsin solution and carries out collagenase treatment, reuses the crosslinking Treatment that crosslinking agent carries out collagen.The corium skin graft after hypertonic salt treatment is handled using alkali lye and mixed solution of hydrogen peroxide, while making in skin graft protein molecular expansion loose, directly go hair removal, save the process of artificial removal's hair, simplification of flowsheet, improving production efficiency saves production cost, reagent used at the same time is nontoxic, does not result in environmental pollution.Using after enzyme is cleared up again by the way of crosslinking Treatment, it is to avoid the destruction of the collagen structure caused and arrangement mode is excessively cleared up, while lifting the toughness of product, elasticity and flexibility.
Description
Technical field
The present invention relates to medical biomaterial technical field, and in particular to a kind of medical acellular dermal matrix and its preparation
Method.
Background technology
For the patient of large-area burns or wound, dermatoplasty is best treatment method, but for burning
Hinder the area especially huge surface of a wound hardly possible using autologous full pachydermia transplanting, and autologous microparticle skin or thin grafts need
Dermis scaffold.The acellular dermis made from the cadaver skin of people is ideal as dermal substitute, but is due to ethics
The reason for the virus such as HIV, it greatly limit its application.At present, acellular dermal base is confirmed according to practice and clinical use
Matter is a kind of new wound repair material, and its application field gradually expands to whole from the treatment of initial deep burn wound
The fields such as shape, beauty, oral cavity, ear nose larynx, department of general surgery.
Purpose prepared by acellular dermal matrix be remove as far as possible in skin histology immunogenicity very strong cell into
Point（Vascular endothelial cell, fibroblast in epidermal cell, hair follicle, sebaceous glands, sweat gland and corium etc.）, retain immunogenicity
The complete three-D space structure of weaker extracellular matrix components and dermal tissue.The life of guide tissue regeneration can be so provided
Thing support, reaches the purpose of repair tissue defect to greatest extent.The preparation method of current acellular dermal matrix mainly has
It is several：1st, Dispase II-Triton methods：The removing of this method cell is thorough, but big to basement membrane destruction, is unfavorable for epithelium thin
The adhesion and growth of born of the same parents；2nd, hypertonic salt-SDS methods：This method basilar memebrane retains complete, but fibronectin, elastin in corium
Equal size is higher, it is thus possible to have higher immunogenicity；3rd, hypertonic salt-sodium hydroxide corrodes method：This method utilizes hypertonic
Salt removes epidermis, while using the water sorption of basic ion in sodium hydroxide, seizing the moisture in histocyte, takes off cell
Water, this method collagen fiber structure is complete, and arrangement is relatively normal loose, but the removal effect for cell still has much room for improvement；4、
Multigelation method, due to being entirely physical operations process, the of long duration, cycle is long, now substantially without.
In above method, effect means are single, and processing time is too short, it is impossible to remove immunogenic substance comprehensively, if
Action time is long, basement membrane destruction can be caused again, and be likely to cause the missing of intact cell skeleton.Meanwhile, artificial physics
Gimmick removes pig hair, wastes time and energy, and takes the producer's a large amount of man-hours.
The content of the invention
In order to overcome the defect of prior art, it is an object of the invention to provide a kind of preparation of medical acellular dermal matrix
Method, can either effectively reduce the immunogenicity of dermal matrix, and the normal collagen structure of dermal matrix is remained again, is used simultaneously
Nontoxic chemical reagent, effectively removes animal hair, and processing procedure is time saving and energy saving, saves production cost, environment is not resulted in again dirty
Dye.
Meanwhile, the present invention, which is also resided in, provides a kind of medical acellular dermal matrix prepared using preparation method of the present invention.
In order to realize the above object the technical solution adopted in the present invention is：
A kind of preparation method of medical acellular dermal matrix, including following operating procedure：
1）After fresh animal skin fleshing, carry out disinfection, clean successively, hypertonic salt treatment, tearing epidermis off, obtain pending corium
Skin graft；
2）By step 1）Obtained corium skin graft, which is put into the mixed solution of alkali lye and hydrogen peroxide, to be soaked 3 ~ 24 hours；
3）By step 2）Corium skin graft after processing cut, is cutd open and is immersed in after piece in the solution containing trypsase, 35 ~
At a temperature of 40 DEG C, pH7 ~ 9 are soaked 1 ~ 12 hour；
4）By step 3）After corium skin graft cleaning after processing, freezing storage completes.
Step 1）Described in animal skin be pigskin, sheepskin or ox-hide.
Above-mentioned preparation method is also included step 3）Corium skin graft after processing, which is immersed in crosslinking agent, carries out normal temperature crosslinked 1
~ 24 hours.Crosslinking Treatment is carried out after enzymic digestion, it is to avoid collagen is degraded excessively in trypsin solution, the collagen caused
The damage of albumen network structure and arrangement mode, it is ensured that the integrality of dermal matrix structure, in the hardness of reduction product, makes product
More soft conformable skin while, make product that there is certain tensile strength, meet the requirement of medical dressing.And crosslinking agent
Use can also further blocking antigen, reduce immune response, the shrinkage temperature of product can also be improved.
Optionally, the compound method of used crosslinking agent is to be added to carbodiimide hydrochloride and succinimide
Containing volumn concentration in 40% ethanol water, pH5.5 stirs.
Optionally, the mass concentration of each component is in used crosslinking agent：Carbodiimide hydrochloride is 0.15%-
2.0%, succinimide concentration is 0.05%-0.5%.
It is preferred that, the mass concentration of each component is in used crosslinking agent：Carbodiimides is 0.5%, succinimide
For 0.2%.
Optionally, step 2）The concentration of alkali is 2 ~ 6% in middle mixed solution；The concentration of hydrogen peroxide is 0.5 ~ 5%.
Optionally, the alkali is sodium hydroxide and/or potassium hydroxide.
In order to ensure the removal effect of alkali lye and hydrogenperoxide steam generator to the hair on corium skin graft, and to egg in skin graft
The expansion loose effect of white fiber, while avoiding the destruction to skin graft collagen space structure and arrangement mode, it is preferred that step 2）
The concentration of sodium hydroxide is 4% in middle mixed solution, and the concentration of hydrogen peroxide is 2%, and the soak time in mixed solution is 18
Hour.
Optionally, step 3）The concentration of trypsase is 0.05 ~ 0.5% in middle trypsin solution.
Optionally, the trypsase is pharmaceutical grade trypsase.
In order to while ensuring that trypsase is cleared up to unnecessary fat and immunocompetence foreign protein, it is to avoid pancreas egg
White destruction of the enzyme to collagen space structure in skin graft, it is to avoid trypsin solution is decomposed or be dissolved in collagen
In, it is further preferred that the concentration of trypsase is 0.1%, the immersion in trypsin solution in the trypsin solution
Time is 2 hours.
Optionally, step 3）In be cut into corium skin graft cut into growth * wide * thickness=10cm ~ 80cm*10cm ~ 80cm*
The skin graft of 0.2mm ~ 1.0mm sizes.
Optionally, step 1）The specific method of middle sterilization is：After fresh animal skin fleshing, be soaked into concentration for 0.5 ~
Disinfected in 1.0% thimerosal 30 ~ 60 minutes.
Optionally, the thimerosal is benzalkonium bromide or Peracetic acid.
Further, it is preferred that the thimerosal is the benzalkonium bromide that concentration is 0.5%, during the processing being immersed in thimerosal
Between be 30 minutes.Inactivation of virus and sterilization processing are carried out to skin surface using thimerosal.
Optionally, step 1）Described in the specific method that cleans be：Use concentration for 0.1 ~ 2% surfactant solution
Animal skin after to disinfecting is cleaned 2 ~ 3 times, every time 0.5 ~ 2 hour.By the effect of surfactant, skin table is removed
The greasy dirt and fat in face.
Optionally, the surfactant is one kind in alkyl polyoxyethylene ether, lauryl sodium sulfate, APG
Or it is a variety of.
It is preferred that, the surfactant is APG1214 or APG1216.
It is further preferred that the APG1214 solution that it is 1% that the surfactant solution, which is concentration,.
Optionally, step 1）The specific method of middle and high infiltration salt treatment is：It is 0.8 that pending animal skin is immersed in into concentration
4 ~ 24 hours in ~ 2.0mol/L sodium chloride solution.
It is preferred that, step 1）The concentration of the sodium chloride used during middle and high infiltration salt treatment is 1.0mol/L, soak time
For 24 hours.The half-bridge kernel structure between epithelial cell and connective tissue is interrupted by high concentration salt solution.
Optionally, step 4）Described in cleaning be using sterile pure water to step 3）Corium skin graft after processing is rinsed
4 ~ 12 hours.
Optionally, step 4）Described in freezing storage to freeze in the environment of -80 DEG C.
The preparation method of the medical acellular dermal matrix of the present invention, is mixed alkali lye and hydrogen peroxide formation by creativeness
Solution is handled the corium skin graft after hypertonic salt treatment, while making in skin graft filament expansion using alkali lye and be loose,
Hair removal is directly gone by the synergy of alkali lye and hydrogen peroxide by the way of chemical immersion, artificial removal's hair is saved
Process, simplification of flowsheet, improving production efficiency saves production cost, and reagent used at the same time is nontoxic, does not result in environment dirty
Dye；
Meanwhile, preparation method of the present invention after alkali lye and hydrogen peroxide carry out unhairing and filament expansion, loose processing to skin graft, then
Trypsin Induced processing is carried out, unnecessary fat is further removed and with immunocompetent foreign protein, removes residual in skin graft
The cell stayed, while by carrying out crosslinking Treatment after enzymic digestion, it is to avoid collagen is degraded excessively in trypsin solution, is made
Into collagen spacial framework and arrangement mode damage, it is ensured that the integrality of dermal matrix structure, reduction product
Hardness, make product more soft conformable skin while, make product that there is certain tensile strength, meet medical dressing will
Ask.And the use of crosslinking agent can also further blocking antigen, reduce immune response, the contraction temperature of product can also be improved
Degree.
Embodiment
Technical scheme is described in detail below by specific embodiment.
Embodiment 1
A kind of preparation method of medical acellular dermal matrix, including following operating procedure：
1）After fresh porcine skin skin fleshing, it is soaked into the benzalkonium bromide solution that concentration is 0.5% and disinfects 30 minutes；
2）By step 1）Animal skin after disinfecting is soaked into the APG1214 solution that concentration is 1%, cleaning 3 times, every time
Cleaning 2 hours；
3）By step 2）Animal skin after cleaning is soaked into the sodium chloride solution that concentration is 1mol/L, is soaked 24 hours, is beaten
Disconnected hemidesmosome between epithelial cell and connective tissue, tears epidermis off, obtains pending corium skin graft；
4）By step 3）Obtained pending corium skin graft is put into the mixed solution containing 4% sodium hydroxide and 2% hydrogen peroxide
In, soak 18 hours；
5）By step 4）Corium skin graft after processing cuts the wide * thickness=10cm ~ 80cm*10cm ~ 80cm*0.2mm ~ 1.0mm of growth *
The skin graft of size；
6）By step 5）Skin graft after cutting is immersed in the solution containing 0.1% trypsase, at a temperature of 37 DEG C, pH7 ~ 9,
Immersion 2 hours；
7）Carbodiimide hydrochloride and succinimide are added to containing 40%（v/v）In the aqueous solution of ethanol, pH5.5 is stirred
Mix uniform, the cross-linking agent solution containing 0.5% carbodiimides and 0.1% succinimide is obtained, then by step 6）Processing
Skin graft afterwards is immersed in cross-linking agent solution, normal temperature crosslinked processing 6 hours；
8）By step 7）After skin graft after processing is rinsed 7 hours using sterile pure water, discharge is neat, freezes the refrigerator at -80 DEG C
In.
Embodiment 2
A kind of preparation method of medical acellular dermal matrix, including following operating procedure：
1）After fresh porcine skin skin fleshing, it is soaked into the peracetic acid soln that concentration is 0.8% and disinfects 45 minutes；
2）By step 1）Animal skin after disinfecting is soaked into the APG1216 solution that concentration is 0.1%, cleaning 3 times, often
Secondary cleaning 1 hour；
3）By step 2）Animal skin after cleaning is soaked into the sodium chloride solution that concentration is 0.8mol/L, is soaked 12 hours,
The hemidesmosome between epithelial cell and connective tissue is interrupted, epidermis is torn off, obtains pending corium skin graft；
4）By step 3）It is molten that obtained pending corium skin graft is put into the mixing containing 2% sodium hydroxide and 0.5% hydrogen peroxide
In liquid, soak 24 hours；
5）By step 4）Corium skin graft after processing cuts the wide * thickness=10cm ~ 80cm*10cm ~ 80cm*0.2mm ~ 1.0mm of growth *
The skin graft of size；
6）By step 5）Skin graft after cutting is immersed in the solution containing 0.05% trypsase, at a temperature of 40 DEG C, pH7 ~ 9,
Immersion 12 hours；
7）Carbodiimide hydrochloride and succinimide are added to containing 40%（v/v）In the aqueous solution of ethanol, pH5.5 is stirred
Mix uniform, the cross-linking agent solution containing 0.15% carbodiimides and 0.05% succinimide is obtained, then by step 6）Place
Skin graft after reason is immersed in cross-linking agent solution, normal temperature crosslinked processing 24 hours；
8）By step 6）After skin graft after processing is rinsed 12 hours using sterile pure water, discharge is neat, freezes the refrigerator at -80 DEG C
In.
Embodiment 3
A kind of preparation method of medical acellular dermal matrix, including following operating procedure：
1）After fresh porcine skin skin fleshing, it is soaked into the benzalkonium bromide solution that concentration is 1.0% and disinfects 60 minutes；
2）By step 1）Animal skin after disinfecting is soaked into the sodium dodecyl sulfate solution that concentration is 2.0%, cleaning
2 times, clean 0.5 hour every time；
3）By step 2）Animal skin after cleaning is soaked into the sodium chloride solution that concentration is 2.0mol/L, is soaked 4 hours, is beaten
Disconnected hemidesmosome between epithelial cell and connective tissue, tears epidermis off, obtains pending corium skin graft；
4）By step 3）Obtained pending corium skin graft is put into the mixed solution containing 6% potassium hydroxide and 5% hydrogen peroxide
In, soak 3 hours；
5）By step 4）Corium skin graft after processing cuts the wide * thickness=10cm ~ 80cm*10cm ~ 80cm*0.2mm ~ 1.0mm of growth *
The skin graft of size；
6）By step 5）Skin graft after cutting is immersed in the solution containing 0.5% trypsase, at a temperature of 35 DEG C, pH7 ~ 9,
Immersion 1 hour；
7）Carbodiimide hydrochloride and succinimide are added to containing 40%（v/v）In the aqueous solution of ethanol, pH5.5 is stirred
Mix uniform, the cross-linking agent solution containing 2.0% carbodiimides and 0.5% succinimide is obtained, then by step 6）Processing
Skin graft afterwards is immersed in cross-linking agent solution, normal temperature crosslinked processing 1 hour；
8）By step 6）After skin graft after processing is rinsed 4 hours using sterile pure water, discharge is neat, freezes the refrigerator at -80 DEG C
In.
The performance of the dermal matrix prepared to above-described embodiment 1 ~ 3 is detected, is as a result shown, embodiment 1 ~ 3 is made
Standby dermal matrix is after natural drying, soft texture, can be good at and skin attachement, and tensile strength is high, artificial free-hand
It is not easy to break during pullling, it can be good at meeting clinical use requirement.
Comparative example 1
A kind of preparation method of medical acellular dermal matrix, including following operating procedure：
1）After fresh porcine skin skin fleshing, it is soaked into the benzalkonium bromide solution that concentration is 0.5% and disinfects 30 minutes；
2）By step 1）Animal skin after disinfecting is soaked into the APG1214 solution that concentration is 1%, cleaning 3 times, every time
Cleaning 2 hours；
3）By step 2）Animal skin after cleaning is soaked into the mixed solution containing 4% sodium hydroxide and 2% hydrogen peroxide,
Immersion 12 hours；
4）By step 3）Animal skin after cleaning is soaked into the sodium chloride solution that concentration is 1mol/L, is soaked 24 hours, is beaten
Disconnected hemidesmosome between epithelial cell and connective tissue, tears epidermis off, obtains pending corium skin graft；
5）By step 4）Obtained pending corium skin graft is put into the alkali lye containing 4% sodium hydroxide, is soaked 12 hours；
5）By step 4）Corium skin graft after processing cuts the wide * thickness=10cm ~ 80cm*10cm ~ 80cm*0.2mm ~ 1.0mm of growth *
The skin graft of size；
6）By step 5）Skin graft after cutting is immersed in the solution containing 0.1% trypsase, at a temperature of 37 DEG C, pH7 ~ 9,
Immersion 2 hours；
7）Carbodiimide hydrochloride and succinimide are added to containing 40%（v/v）In the aqueous solution of ethanol, pH5.5 is stirred
Mix uniform, the cross-linking agent solution containing 0.5% carbodiimides and 0.1% succinimide is obtained, then by step 6）Processing
Skin graft afterwards is immersed in cross-linking agent solution, normal temperature crosslinked processing 6 hours；
8）By step 7）After skin graft after processing is rinsed 7 hours using sterile pure water, discharge is neat, freezes the refrigerator at -80 DEG C
In.
Skin is immersed in the mixing of alkali lye and hydrogen peroxide composition compared to embodiment 1 by this comparative example before hypertonic salt treatment
In solution, observation result shows that this mode of operation can not remove the hair remained in hair removal, especially corium pore well
Hair is, it is necessary to be additionally carried out the process of artificial removal's hair.
Comparative example 2
A kind of preparation method of medical acellular dermal matrix, including following operating procedure：
1）After fresh porcine skin skin fleshing, it is soaked into the benzalkonium bromide solution that concentration is 0.5% and disinfects 30 minutes；
2）By step 1）Animal skin after disinfecting is soaked into the APG1214 solution that concentration is 1%, cleaning 3 times, every time
Cleaning 2 hours；
3）By step 2）Animal skin after cleaning is soaked into the sodium chloride solution that concentration is 1mol/L, is soaked 24 hours, is beaten
Disconnected hemidesmosome between epithelial cell and connective tissue, tears epidermis off, obtains pending corium skin graft；
4）By step 3）Obtained pending corium skin graft be put into the alkali lye containing 4% sodium hydroxide soak 12 hours after, then
In 2% hydrogenperoxide steam generator, soak 12 hours；
5）By step 4）Corium skin graft after processing cuts the wide * thickness=10cm ~ 80cm*10cm ~ 80cm*0.2mm ~ 1.0mm of growth *
The skin graft of size；
6）By step 5）Skin graft after cutting is immersed in the solution containing 0.1% trypsase, at a temperature of 37 DEG C, pH7 ~ 9,
Immersion 2 hours；
7）Carbodiimide hydrochloride and succinimide are added to containing 40%（v/v）In the aqueous solution of ethanol, pH5.5 is stirred
Mix uniform, the cross-linking agent solution containing 0.5% carbodiimides and 0.1% succinimide is obtained, then by step 6）Processing
Skin graft afterwards is immersed in cross-linking agent solution, normal temperature crosslinked processing 6 hours；
8）By step 7）After skin graft after processing is rinsed 7 hours using sterile pure water, discharge is neat, freezes the refrigerator at -80 DEG C
In.
This comparative example as different from Example 1, the corium skin graft after hypertonic salt treatment is separately immersed in alkali lye
After the loose processing of row expansion, then it is immersed in hydrogenperoxide steam generator, observation result shows that this mode of operation can not be good
Hair removal is gone, it is necessary to carry out the extra operating procedure for manually removing hair.
Comparative example 3
A kind of preparation method of medical acellular dermal matrix, including following operating procedure：
1）After fresh porcine skin skin fleshing, it is soaked into the benzalkonium bromide solution that concentration is 0.5% and disinfects 30 minutes；
2）By step 1）Animal skin after disinfecting is soaked into the APG1214 solution that concentration is 1%, cleaning 3 times, every time
Cleaning 2 hours；
3）By step 2）Animal skin after cleaning is soaked into the sodium chloride solution that concentration is 1mol/L, is soaked 24 hours, is beaten
Disconnected hemidesmosome between epithelial cell and connective tissue, tears epidermis off, obtains pending corium skin graft；
4）By step 3）Obtained pending corium skin graft is put into the mixed solution containing 4% sodium hydroxide and 2% hydrogen peroxide
In, soak 18 hours；
5）By step 4）Corium skin graft after processing cuts the wide * thickness=10cm ~ 80cm*10cm ~ 80cm*0.2mm ~ 1.0mm of growth *
The skin graft of size；
6）By step 5）Skin graft after cutting is immersed in the solution containing 0.1% trypsase, at a temperature of 37 DEG C, pH7 ~ 9,
Immersion 2 hours；
7）By step 6）After skin graft after processing is rinsed 7 hours using sterile pure water, discharge is neat, freezes the refrigerator at -80 DEG C
In.
The difference of this comparative example and embodiment 1 is, is not crosslinked after collagenase treatment in this comparative example preparation method
Processing, the result for comparing two kinds of properties of product shows that product tensile strength prepared by this comparative example is low, it is easy to pull apart, it is impossible to
The need for normal satisfaction is clinical, product can be hardened after spontaneously drying, and can also bring uncomfortable sensation during use to patient.
Claims (10)
1. a kind of preparation method of medical acellular dermal matrix, it is characterised in that including following operating procedure：
1）After fresh animal skin fleshing, carry out disinfection, clean successively, hypertonic salt treatment, tearing epidermis off, obtain pending corium
Skin graft；
2）By step 1）Obtained corium skin graft, which is put into the mixed solution of alkali lye and hydrogen peroxide, to be soaked 3 ~ 24 hours；
3）By step 2）Corium skin graft after processing cut, is cutd open and is immersed in after layer in the solution containing trypsase, 35 ~
At a temperature of 40 DEG C, pH7 ~ 9 are soaked 1 ~ 12 hour；
4）By step 3）After corium skin graft cleaning after processing, freezing storage completes.
2. the preparation method of medical acellular dermal matrix as claimed in claim 1, it is characterised in that also including by step 3）
Corium skin graft after processing, which is immersed in crosslinking agent, to be carried out normal temperature crosslinked 1 ~ 24 hour.
3. the preparation method of medical acellular dermal matrix as claimed in claim 2, it is characterised in that used crosslinking agent
Compound method be by carbodiimide hydrochloride and succinimide be added to containing volumn concentration be 40% ethanol water
In solution, pH5.5 stirs；The mass concentration of each component is in used crosslinking agent：Carbodiimide hydrochloride is
0.15% ~ 2.0%, succinimide concentration is 0.05% ~ 0.5%.
4. the preparation method of the medical acellular dermal matrix as described in any one of claim 1 ~ 3, it is characterised in that step 2）
The concentration of alkali is 2 ~ 6% in middle mixed solution；The concentration of hydrogen peroxide is 0.5 ~ 5%；The alkali is sodium hydroxide and/or hydroxide
Potassium.
5. the preparation method of the medical acellular dermal matrix as described in any one of claim 1 ~ 3, it is characterised in that step 3）
The concentration of trypsase is 0.05 ~ 0.5% in middle trypsin solution.
6. the preparation method of the medical acellular dermal matrix as described in any one of claim 1 ~ 3, it is characterised in that step 3）
In cut, cut open layer be by corium skin graft cut growth the wide * thickness=10cm ~ 80cm*10cm ~ 80cm*0.2mm ~ 1.0mm sizes of * skin
Piece.
7. the preparation method of the medical acellular dermal matrix as described in any one of claim 1 ~ 3, it is characterised in that step 1）
The specific method of middle sterilization is：After fresh animal skin fleshing, it is soaked into the thimerosal that concentration is 0.5 ~ 1.0% at sterilization
Reason 30 ~ 60 minutes；The thimerosal is benzalkonium bromide or Peracetic acid.
8. the preparation method of the medical acellular dermal matrix as described in any one of claim 1 ~ 3, it is characterised in that step 1）
Described in the specific method that cleans be：Use concentration for 0.1 ~ 2% surfactant solution to disinfecting after animal skin
Cleaning 2 ~ 3 times, every time 0.5 ~ 2 hour；The surfactant is alkyl polyoxyethylene ether, lauryl sodium sulfate, alkyl sugar
One or more in glycosides.
9. the preparation method of the medical acellular dermal matrix as described in any one of claim 1 ~ 3, it is characterised in that step 1）
The specific method of middle and high infiltration salt treatment is：Pending animal skin is immersed in the sodium chloride solution that concentration is 0.8 ~ 2.0mol/L
In 4 ~ 24 hours.
10. a kind of medical acellular dermal matrix, it is characterised in that as the preparation method as described in any one of claim 1 ~ 9
Prepare.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710247775.2A CN107029298A (en) | 2017-04-17 | 2017-04-17 | A kind of medical acellular dermal matrix and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710247775.2A CN107029298A (en) | 2017-04-17 | 2017-04-17 | A kind of medical acellular dermal matrix and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107029298A true CN107029298A (en) | 2017-08-11 |
Family
ID=59535341
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710247775.2A Pending CN107029298A (en) | 2017-04-17 | 2017-04-17 | A kind of medical acellular dermal matrix and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107029298A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109675112A (en) * | 2019-02-22 | 2019-04-26 | 上海仁康科技有限公司 | A kind of preparation method of the acellular dermal matrix of source of people |
| CN110433340A (en) * | 2019-07-26 | 2019-11-12 | 广州聚明生物科技有限公司 | Acellular matrix urethral slings repair materials and its preparation method and application |
| CN114377207A (en) * | 2022-01-19 | 2022-04-22 | 南方医科大学南方医院 | Preparation method and application of porcine acellular dermal matrix scaffold |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178981A (en) * | 2011-04-20 | 2011-09-14 | 北京市创伤骨科研究所 | Method for preparing cartilage repairing scaffold material |
| CN102218162A (en) * | 2011-05-24 | 2011-10-19 | 山西奥瑞生物材料有限公司 | Preparation method of homologous acellular dermal matrix |
| CN103785065A (en) * | 2014-01-24 | 2014-05-14 | 北京大清生物技术有限公司 | Preparation method of cartilage regeneration scaffold material |
| CN104587530A (en) * | 2015-01-15 | 2015-05-06 | 四川大学 | Medical purified pig dermis and preparation method thereof |
| CN105079880A (en) * | 2015-09-01 | 2015-11-25 | 河北爱能生物科技股份有限公司 | Preparing method of heterogeneous acellular dermal matrix substrate with good biocompatibility |
| CN105126170A (en) * | 2015-08-18 | 2015-12-09 | 深圳兰度生物材料有限公司 | Acellular dermal matrix and preparing method of acellular dermal matrix |
-
2017
- 2017-04-17 CN CN201710247775.2A patent/CN107029298A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178981A (en) * | 2011-04-20 | 2011-09-14 | 北京市创伤骨科研究所 | Method for preparing cartilage repairing scaffold material |
| CN102218162A (en) * | 2011-05-24 | 2011-10-19 | 山西奥瑞生物材料有限公司 | Preparation method of homologous acellular dermal matrix |
| CN103785065A (en) * | 2014-01-24 | 2014-05-14 | 北京大清生物技术有限公司 | Preparation method of cartilage regeneration scaffold material |
| CN104587530A (en) * | 2015-01-15 | 2015-05-06 | 四川大学 | Medical purified pig dermis and preparation method thereof |
| CN105126170A (en) * | 2015-08-18 | 2015-12-09 | 深圳兰度生物材料有限公司 | Acellular dermal matrix and preparing method of acellular dermal matrix |
| CN105079880A (en) * | 2015-09-01 | 2015-11-25 | 河北爱能生物科技股份有限公司 | Preparing method of heterogeneous acellular dermal matrix substrate with good biocompatibility |
Non-Patent Citations (1)
| Title |
|---|
| 柴家科: "《实用烧伤外科学》", 30 September 2014, 人民军医出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109675112A (en) * | 2019-02-22 | 2019-04-26 | 上海仁康科技有限公司 | A kind of preparation method of the acellular dermal matrix of source of people |
| CN110433340A (en) * | 2019-07-26 | 2019-11-12 | 广州聚明生物科技有限公司 | Acellular matrix urethral slings repair materials and its preparation method and application |
| CN110433340B (en) * | 2019-07-26 | 2022-04-22 | 广州聚明生物科技有限公司 | Acellular matrix urethral suspension repair material and preparation method and application thereof |
| CN114377207A (en) * | 2022-01-19 | 2022-04-22 | 南方医科大学南方医院 | Preparation method and application of porcine acellular dermal matrix scaffold |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3040088B1 (en) | Method for preparing an animal decellularized tissue matrix material and a decellularized tissue matrix material prepared thereby | |
| CA2173547C (en) | A raw membranous material for medical materials and manufacturing methods thereof | |
| JP4558107B2 (en) | Chemical cleaning of biological materials | |
| JP2020171735A (en) | Method for enzymatic treatment of tissue products | |
| CN104524634B (en) | Preparation method of tissue repair material | |
| US20100112543A1 (en) | Processing soft tissue, methods and compositions related thereto | |
| JP2018523467A (en) | Production and use of high purity collagen particles | |
| CN107029298A (en) | A kind of medical acellular dermal matrix and preparation method thereof | |
| CN109364298A (en) | A kind of preparation method of acellular dermal matrix material | |
| CN109675112B (en) | Preparation method of human-derived acellular dermal matrix | |
| CN112618796B (en) | Acellular dermal matrix material and preparation method and application thereof | |
| CN107412867B (en) | Preparation method of heterogenous acellular dermal matrix | |
| CA2677229A1 (en) | Decellularization of soft tissue | |
| CN114796615A (en) | Cartilage acellular matrix and preparation method thereof | |
| US20210069381A1 (en) | Methods and systems for stiffening of tissue for improved processing | |
| JPH0550295B2 (en) | ||
| WO2020062350A1 (en) | Acellular biological amnion containing loose layer and preparation method therefor | |
| TWI763265B (en) | An organ-repairing membrane with structural proteins and use thereof | |
| CN110772665B (en) | Method for removing heterogeneous corneal cells | |
| WO2023214361A1 (en) | Method for decellularization of a tissue, decellularized tissue matrix and a scaffold for use thereof in tissue repair | |
| CN110960731A (en) | Preparation method of medical surgical biological patch | |
| CN114146224A (en) | Preparation method of acellular dermal matrix particles | |
| AU774997B2 (en) | Chemical cleaning of biological material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170811 |
|
| RJ01 | Rejection of invention patent application after publication |