CN108402255A - A kind of Camellia nitidissima leaf Hypoylycemic health tea and its preparation method and application - Google Patents
A kind of Camellia nitidissima leaf Hypoylycemic health tea and its preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
Abstract
The present invention proposes a kind of preparation method of Camellia nitidissima leaf Hypoylycemic health tea, includes the following steps：(1) root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are sorted, dries, crushes, be sieved and the mixing of bionic extraction liquid, 37 DEG C of heating water baths, diaphragm seal, magneton extraction, filtering collect filtrate, be freeze-dried, obtain composition A；(2) it by Camellia nitidissima leaf, Qingqian Willow leaf wash clean, dries, crushes, fermentation, frying water-removing obtains composition B；(3) composition A powder and composition B powder are mixed, drying obtains dry tea powder；(4) step dry tea powder is packed into the non-woven fabrics tea bag after sterilizing and drying, is sealed after drawing a cotton thread, Camellia nitidissima leaf Hypoylycemic health tea is made.The health protection tea that the present invention is prepared into is of low cost, has no toxic side effect, and long-term drinking being capable of apparent hypoglycemic effect.
Description
Technical field
The present invention relates to a plant teas, and in particular to a kind of Hypoylycemic health tea.
Background technology
When (sugar-free and any food intake containing sugar in 8 hours) blood glucose is higher than normal range (NR), referred to as hyperglycemia on an empty stomach.Normally
In the case of, human body can ensure that the source of blood glucose keeps flat with outlet by hormone control and nerve modulation this two big regulating system
Weighing apparatus, makes blood glucose maintain certain level.But in inherent cause (such as Diabetes family history) and environmental factor (such as unreasonable meals
Food, obesity etc.) collective effect under, two big regulatory functions get muddled, and just will appear the raising of blood glucose level.Long-term high blood
Sugar can make each histoorgan of whole body that lesion occur, and lead to the generation of acute and chronic complication.Such as pancreas function failure, dehydration, electricity
Solve matter disorder, nutritional deficiency, resistance decline, impaired renal function, neuropathy, eyeground pathological changes etc..
The current drug for being used clinically for diabetes caused by treatment hyperglycemia is mainly including insulin injection etc..High agent
Amount insulin forces peripheral tissues' (such as fat and muscle) intake sugar to achieve the purpose that reduce blood glucose.Its advantage is that blood sugar reducing function
It is fast and apparent；The disadvantage is that body unavoidably will produce insulin dependence, the side effects such as hypoglycemic shock, and finally due to
Atrophy of pancreas and islet function completely loses caused by insulin relies on.Meanwhile regular iletin is at high price, to sugar
Urine patient brings heavy financial burden.
Golden flower Camellia Theaceae Camellia Camellia Section Chrysanthas, are known as " tea race queen " and " plant kingdom giant panda ".Gold
A kind of Chinese herbal medicine of the jasmine tea leaf as Zhuang nationality in Guangxi folk tradition has hypoglycemic, reducing blood lipid, inhibits tumour growth, improves machine
The different physiological roles such as body immunocompetence, wherein camellia chrysantha polysaccharide is considered as important one of effect ingredient.
Invention content
In order to solve the above technical problems, the present invention provides a kind of preparation method of Camellia nitidissima leaf Hypoylycemic health tea
And application, the preparation method is simple to operation, can extract the main composition of composition, the health protection tea being prepared into
It is of low cost, it has no toxic side effect, long-term drinking being capable of apparent hypoglycemic effect.
The present invention provides a kind of preparation method of Camellia nitidissima leaf Hypoylycemic health tea, includes the following steps：
(1) root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are sorted, dries, crushes, cross 60-120 mesh sieve, obtained powder and bionical
Extracting solution is according to weight ratio 1:The ratio of 10-50 mixes, 37 DEG C of heating water baths, and plastic film sealing, magneton extracts 100-
Filtrate is collected in 180min, filtering, is freeze-dried, and is crushed, is obtained composition A powder；
(2) by Camellia nitidissima leaf, Qingqian Willow leaf wash clean, 30-40 DEG C of constant-temperature vacuum drying crushes, is packed into sealing container
200-300 DEG C of frying water-removing 5-10min after 40-60 DEG C of fermentation 5-10 days, obtains composition B powder；
(3) composition A powder in step (1) and (2) and composition B powder are mixed, continues 40-60 DEG C of constant-temperature vacuum and dries
It does to water content<5-10% obtains dry tea powder；
(4) the dry tea powder for obtaining step (3) is packed into the non-woven fabrics tea bag after sterilizing and drying, is sealed after drawing a cotton thread
Mouthful, Camellia nitidissima leaf Hypoylycemic health tea is made.
It is further improved as of the invention, a kind of preparation method of Camellia nitidissima leaf Hypoylycemic health tea, including following
Step：
(1) root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are sorted, dries, crushes, cross 80 mesh sieve, obtained powder and bionic extraction
Liquid is according to weight ratio 1:30 ratio mixing, 37 DEG C of heating water baths, plastic film sealing, magneton extract 120min, filter, collect
Filtrate is freeze-dried, and crushes, obtains composition A powder；
(2) by Camellia nitidissima leaf, Qingqian Willow leaf wash clean, 35 DEG C of constant-temperature vacuum drying crush, are packed into 50 DEG C of sealing container
270 DEG C of fryings water-removing 8min after fermentation 7 days, obtain composition B powder；
(3) composition A powder in step (1) and (2) and composition B powder are mixed, continues 50 DEG C of constant-temperature vacuum drying
To water content<7%, obtain dry tea powder；
(4) the dry tea powder for obtaining step (3) is packed into the non-woven fabrics tea bag after sterilizing and drying, is sealed after drawing a cotton thread
Mouthful, Camellia nitidissima leaf Hypoylycemic health tea is made.
It is improved as of the invention further, tea leaf fermentation degree is in 20-50% when fermentation step.
Improved as of the invention further, bionic extraction liquid by 0.2% sodium chloride, 0.32% pepsin and
0.02mol/L hydrochloric acid configures.
As further improvement of the invention, 0.2% sodium chloride of bionic extraction liquid：0.32% pepsin：0.02mol/L
Hydrochloric acid is 1:1:2.
The present invention further protects a kind of Camellia nitidissima leaf Hypoylycemic health tea prepared by the above method, by following weight
The raw material of part is prepared：20-30 parts of Camellia nitidissima leaf, 15-20 parts of blue or green money willow, 15-20 parts of the root bark of Chinese wolf-berry, epipremnum aureum spend 10-15 parts,
7-12 parts of anchor of flower.
As further improvement of the invention, a kind of Camellia nitidissima leaf Hypoylycemic health tea, by the raw material of following parts by weight
It is prepared：25 parts of Camellia nitidissima leaf, 17 parts of blue or green money willow, 17 parts of the root bark of Chinese wolf-berry, epipremnum aureum spend 10 parts 12 parts, flower of anchor.
The present invention further protects a kind of application of above-mentioned Camellia nitidissima leaf Hypoylycemic health tea.
The present invention has the advantages that：
1, experiments have shown that the bioactive substance that the polysaccharide type organic of golden camellia tea has has a variety of pharmacological effects,
There is apparent hypoglycemic effect.
2, Camellia nitidissima plantation is simple, and yield is big, and the health protection tea that it is prepared into together with other compositions is of low cost, nothing
Toxic side effect, long-term drinking being capable of apparent hypoglycemic effects.
Description of the drawings
Fig. 1 is the preparation technology figure of Camellia nitidissima leaf Hypoylycemic health tea.
Specific implementation mode
Below in conjunction with the embodiment of the present invention, technical solution in the embodiment of the present invention carries out clear, complete description,
Obviously, the embodiment described is the embodiment of the part of representative of the present invention, rather than whole embodiments, this field are general
Other all embodiments that logical technical staff is obtained without making creative work belong to the protection of the present invention
Range.
The preparation of 1 golden camellia leaf Hypoylycemic health tea of embodiment
Raw material forms：20 parts of Camellia nitidissima leaf, 15 parts of blue or green money willow, 15 parts of the root bark of Chinese wolf-berry, epipremnum aureum spend 7 parts 10 parts, flower of anchor；
It prepares according to the following steps：
(1) root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are sorted, dries, crushes, cross 60 mesh sieve, obtained powder and bionic extraction
Liquid is according to weight ratio 1:10 ratio mixing, 37 DEG C of heating water baths, plastic film sealing, magneton extract 100min, filter, collect
Filtrate is freeze-dried, and crushes, obtains composition A powder, yield 67%；
(2) by Camellia nitidissima leaf, Qingqian Willow leaf wash clean, 30 DEG C of constant-temperature vacuum drying crush, are packed into 40 DEG C of sealing container
200-300 DEG C of frying water-removing 5min after fermentation 5 days, obtains composition B powder, yield 70%；
(3) composition A powder in step (1) and (2) and composition B powder are mixed, continues 40 DEG C of constant-temperature vacuum drying
To water content<10%, obtain dry tea powder；
(4) the dry tea powder for obtaining step (3) is packed into the non-woven fabrics tea bag after sterilizing and drying, is sealed after drawing a cotton thread
Mouthful, Camellia nitidissima leaf Hypoylycemic health tea is made.
The preparation of 2 golden camellia leaf Hypoylycemic health tea of embodiment
Raw material forms：25 parts of Camellia nitidissima leaf, 17 parts of blue or green money willow, 17 parts of the root bark of Chinese wolf-berry, epipremnum aureum spend 10 parts 12 parts, flower of anchor；
It prepares according to the following steps：
(1) root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are sorted, dries, crushes, cross 80 mesh sieve, obtained powder and bionic extraction
Liquid is according to weight ratio 1:30 ratio mixing, 37 DEG C of heating water baths, plastic film sealing, magneton extract 120min, filter, collect
Filtrate is freeze-dried, and crushes, obtains composition A powder, yield 89%；
(2) by Camellia nitidissima leaf, Qingqian Willow leaf wash clean, 35 DEG C of constant-temperature vacuum drying crush, are packed into 50 DEG C of sealing container
270 DEG C of fryings water-removing 8min after fermentation 7 days, obtain composition B powder, yield 92%；
(3) composition A powder in step (1) and (2) and composition B powder are mixed, continues 50 DEG C of constant-temperature vacuum drying
To water content<7%, obtain dry tea powder；
(4) the dry tea powder for obtaining step (3) is packed into the non-woven fabrics tea bag after sterilizing and drying, is sealed after drawing a cotton thread
Mouthful, Camellia nitidissima leaf Hypoylycemic health tea is made.
The preparation of 3 golden camellia leaf Hypoylycemic health tea of embodiment
Raw material forms：30 parts of Camellia nitidissima leaf, 20 parts of blue or green money willow, 20 parts of the root bark of Chinese wolf-berry, epipremnum aureum spend 12 parts 15 parts, flower of anchor；
It follows the steps below：
(1) root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are sorted, dries, crushes, cross 120 mesh sieve, obtained powder and bionic extraction
Liquid is according to weight ratio 1:50 ratio mixing, 37 DEG C of heating water baths, plastic film sealing, magneton extract 180min, filter, collect
Filtrate is freeze-dried, and crushes, obtains composition A powder, yield 82%；
(2) by Camellia nitidissima leaf, Qingqian Willow leaf wash clean, 40 DEG C of constant-temperature vacuum drying crush, are packed into 60 DEG C of sealing container
300 DEG C of fryings water-removing 10min after fermentation 10 days, obtain composition B powder, yield 85%；
(3) composition A powder in step (1) and (2) and composition B powder are mixed, continues 40-60 DEG C of constant-temperature vacuum and dries
It does to water content<5%, obtain dry tea powder；
(4) the dry tea powder for obtaining step (3) is packed into the non-woven fabrics tea bag after sterilizing and drying, is sealed after drawing a cotton thread
Mouthful, Camellia nitidissima leaf Hypoylycemic health tea is made.
1 commonsense method of reference examples extraction tea obtained
Raw material forms：25 parts of Camellia nitidissima leaf, 17 parts of blue or green money willow, 17 parts of the root bark of Chinese wolf-berry, epipremnum aureum spend 10 parts 12 parts, flower of anchor；
Operating procedure：
(1) each component is weighed by weight；
(2) Camellia nitidissima leaf, blue or green money willow, the root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are dried, is crushed, sieving adds 10 times of volumes
The water of 8 times of volumes, refluxing extraction 2h is added in water, refluxing extraction 2h, filtering, filter residue, and filtering merges filtrate twice, is concentrated to
Density is 0.9-1.3；
(3) concentrate for obtaining step (2) is freeze-dried, and is distributed into tea bag, is sealed after drawing a cotton thread, is made and is protected
Strong tea bag.
The amylase of reference examples 2 and pectase extraction tea obtained
Raw material forms：25 parts of Camellia nitidissima leaf, 17 parts of blue or green money willow, 17 parts of the root bark of Chinese wolf-berry, epipremnum aureum spend 10 parts 12 parts, flower of anchor；
It carries out according to the following steps：
(1) Camellia nitidissima leaf, blue or green money willow, the root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are taken by weight, are crushed, are mixed, sieving, gained
Traditional Chinese medicine powder be added to the water by a certain percentage, stir evenly, in distilled water, be added phosphate buffer by a certain percentage, be added and form sediment
Powder enzyme (200U/g) and pectase (350U/g), 400r/min is stirred to react 5h at a temperature of 50 DEG C, and reaction is completed, and is centrifuged, and is collected
Supernatant concentrates, and freeze-drying obtains Hypoylycemic health tea enzymatic hydrolyzed extract；
(2) extract is distributed into tea bag, is sealed after drawing a cotton thread, health care tea bag is made.
1 mouse test of test example
1, group is tested.Take mouse similar in 50 sizes, be divided into 5 groups, respectively experimental group, 1 group of control, 2 groups of control,
Compare 3 groups and blank group, every group 10.
2, test method.Hyperglycemia Establishment of mouse model and processing.Experimental group, 1 group of control, 2 groups of control and control are 3 groups small
Mouse gives tail vein injection 10mg/m L alloxans, dosage 50mg/kg.After 3d, tail vein blood surveys blood glucose.Blood glucose value exists
The mouse of 10mmol/L or more is the hyperglycemia mouse of successfully modeling.
Camellia nitidissima leaf Hypoylycemic health tea packet prepared by the embodiment 2 of 10g, the extraction of 1 commonsense method of reference examples is taken to be made
Tea bag and the amylase of reference examples 2 and pectase extraction tea bag obtained, 100mL brewed in hot water is added, filtering obtains corresponding
Tea.
Experimental mice gives the tea that the Camellia nitidissima leaf Hypoylycemic health tea of the preparation of tail vein injection embodiment 2 steeps
100g/kg compares the tea 100g/kg that 1 group of mouse injection 1 commonsense method of reference examples extraction tea bag obtained steeps, compares 2 groups
Mouse injects the tea 100g/kg that the amylase of reference examples 2 and pectase extraction tea bag obtained steep, and compares 3 groups and blank
Group mouse injects equivalent distilled water.Blood glucose is surveyed after 12h.
GOD-POD methods survey blood glucose.Saturated oxalic acid potassium solution (anti-coagulants) rinse pipette tips and 0.5m LDorf pipes, blood sampling,
3000r/min centrifuges 30min, and supernatant is taken to carry out blood sugar detection with glucose kit.
Experimental result is referring to table 1.
1 each group mouse blood sugar decreasing effect table of table
It can be seen from the above result that tea bag prepared by tea and the extraction of other enzymes that the tea bag prepared with commonsense method steeps steeps
The tea obtained is compared, and the tea that Camellia nitidissima leaf Hypoylycemic health tea prepared by embodiment 2 steeps can in a short time significantly
The blood glucose value for reducing mouse, is allowed to be restored within the scope of normal level.
2 human trial of test example
1, group is tested.Be divided into 5 groups, every group of 20 people, respectively experimental group, blank control group, 1 group of control, 2 groups of control and
Compare 3 groups.
2, experimental method.
(1) case selection：It is volunteer to participate in experimenter, and blood glucose is diagnosed as simultaneously by 3 internal clinic section doctors
Higher patient.All patients start to deactivate hypoglycemic drug or health products in first 2 weeks in experiment, screen case 100 altogether, wherein
Male 45, women 55, age 39-59 Sui, average age (47.5 years old ± 4.7) year.
Inclusion criteria：Continue to measure a fasting blood-glucose in and be above 8mmol/l, is less than 10mmol/l.
(2) experimental method：All tested volunteer is randomly divided into 5 groups, and every group of 20 people, experimental group takes the Camellia nitidissima of preparation
The tea that 1 commonsense method of reference examples extraction tea bag obtained steeps is taken in the tea that leaf Hypoylycemic health tea steeps, 1 group of control
The tea that the amylase of reference examples 2 and pectase extraction tea bag obtained steep is taken in water, 2 groups of control, and compound is taken in 3 groups of control
Yin-nourishing melbine, blank control group take plain boiled water.Instructions of taking, twice daily, each 100mL compare 3 groups twice daily,
After 1 tablet once, and 30 days as a treatment course, follow-up is primary weekly, records the fasting blood-glucose index of every patient, and inquiry is taken
The body-sensing of patient.
(3) evaluation criterion：It is recovery from illness that blood glucose, which is down to 3.9-6.1mmol/l, and it is effective, blood that blood glucose, which is down to 6.1-7mmol/l,
It is to improve that sugar, which is down to 7-8mmol/l, and blood glucose is still invalid in 8-10mmol/l.Total effective rate=recovery from illness+effective+improvement.
Experimental result is referring to table 2.
2 blood sugar decreasing effect table of table
| Group | n | Recovery from illness | It is effective | It improves | In vain | Total effective rate % |
| Experimental group | 20 | 7 | 10 | 2 | 1 | 95 |
| Compare 1 group | 20 | 3 | 4 | 4 | 9 | 55 |
| Compare 2 groups | 20 | 5 | 3 | 5 | 7 | 65 |
| Compare 3 groups | 20 | 5 | 7 | 4 | 4 | 80 |
| Blank control group | 20 | 0 | 0 | 2 | 18 | 10 |
It can be seen from the above result that the blood sugar decreasing effect of Camellia nitidissima leaf Hypoylycemic health tea prepared by the embodiment of the present invention 2
It is better than the tea of Traditional Chinese medicine for reducing blood glucose herbal mixture yin-nourishing melbine and the tea and the extraction preparation of other enzymes that are prepared with commonsense method,
Golden camellia leaf and other compositions are prepared into health protection tea using the method for bionic extraction, blood sugar decreasing effect is apparent, takes for a long time,
Contribute to the health of hyperglycemic patients.
Those skilled in the art is not under conditions of departing from the spirit and scope of the present invention of claims determination, also
Various modifications can be carried out to the above content.Therefore the scope of the present invention is not limited in above explanation, but by
The range of claims determines.
Claims (8)
1. a kind of preparation method of Camellia nitidissima leaf Hypoylycemic health tea, which is characterized in that include the following steps：
(1) root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are sorted, dries, crushes, cross 60-120 mesh sieve, obtained powder and bionic extraction
Liquid is according to weight ratio 1:The ratio of 10-50 mixes, 37 DEG C of heating water baths, and plastic film sealing, magneton extracts 100-180min, mistake
Filtrate is collected in filter, is freeze-dried, and is crushed, is obtained composition A powder；
(2) by Camellia nitidissima leaf, Qingqian Willow leaf wash clean, 30-40 DEG C of constant-temperature vacuum drying crushes, is packed into sealing container 40-60
200-300 DEG C of frying water-removing 5-10min after DEG C fermentation 5-10 days, obtains composition B powder；
(3) composition A powder in step (1) and (2) and composition B powder are mixed, continues 40-60 DEG C of constant-temperature vacuum and is dried to
Water content<5-10% obtains dry tea powder；
(4) the dry tea powder for obtaining step (3) is packed into the non-woven fabrics tea bag after sterilizing and drying, is sealed after drawing a cotton thread, makes
Obtain Camellia nitidissima leaf Hypoylycemic health tea.
2. a kind of preparation method of Camellia nitidissima leaf Hypoylycemic health tea according to claim 1, which is characterized in that including with
Lower step：
(1) root bark of Chinese wolf-berry, epipremnum aureum flower, flower anchor are sorted, dries, crushes, cross 80 mesh sieve, obtained powder and bionic extraction liquid is pressed
According to weight ratio 1:30 ratio mixing, 37 DEG C of heating water baths, plastic film sealing, magneton extract 120min, and filter is collected in filtering
Liquid is freeze-dried, and is crushed, is obtained composition A powder；
(2) by Camellia nitidissima leaf, Qingqian Willow leaf wash clean, 35 DEG C of constant-temperature vacuum drying crush, and are packed into 50 DEG C of fermentations of sealing container
270 DEG C of fryings water-removing 8min, obtain composition B powder after 7 days；
(3) composition A powder in step (1) and (2) and composition B powder are mixed, 50 DEG C of constant-temperature vacuums of continuation, which are dried to, to be contained
Water<7%, obtain dry tea powder；
(4) the dry tea powder for obtaining step (3) is packed into the non-woven fabrics tea bag after sterilizing and drying, is sealed after drawing a cotton thread, makes
Obtain Camellia nitidissima leaf Hypoylycemic health tea.
3. a kind of preparation method of Camellia nitidissima leaf Hypoylycemic health tea according to claim 1 or claim 2, which is characterized in that institute
Tea leaf fermentation degree is in 20-50% when stating fermentation step.
4. a kind of preparation method of Camellia nitidissima leaf Hypoylycemic health tea according to claim 1 or claim 2, which is characterized in that institute
Bionic extraction liquid is stated to be configured by 0.2% sodium chloride, 0.32% pepsin and 0.02mol/L hydrochloric acid.
5. a kind of preparation method of Camellia nitidissima leaf Hypoylycemic health tea according to claim 4, which is characterized in that described imitative
0.2% sodium chloride of raw extracting solution：0.32% pepsin：0.02mol/L hydrochloric acid is 1:1:2.
6. the Camellia nitidissima leaf Hypoylycemic health tea prepared according to claim 1, which is characterized in that by the raw material of following parts by weight
It is prepared：20-30 parts of Camellia nitidissima leaf, 15-20 parts of blue or green money willow, 15-20 parts of the root bark of Chinese wolf-berry, epipremnum aureum spend 10-15 parts, flower anchor 7-12
Part.
7. a kind of Camellia nitidissima leaf Hypoylycemic health tea according to claim 6, which is characterized in that by the original of following parts by weight
Material is prepared：25 parts of Camellia nitidissima leaf, 17 parts of blue or green money willow, 17 parts of the root bark of Chinese wolf-berry, epipremnum aureum spend 10 parts 12 parts, flower of anchor.
8. a kind of application of Camellia nitidissima leaf Hypoylycemic health tea described according to claim 6 or 7.
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