CN108402057A - Application of the indole carbazole compound in preparing resisting banana vascular wilt cause of disease microbial inoculum - Google Patents
Application of the indole carbazole compound in preparing resisting banana vascular wilt cause of disease microbial inoculum Download PDFInfo
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- CN108402057A CN108402057A CN201810185161.0A CN201810185161A CN108402057A CN 108402057 A CN108402057 A CN 108402057A CN 201810185161 A CN201810185161 A CN 201810185161A CN 108402057 A CN108402057 A CN 108402057A
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- Prior art keywords
- microbial inoculum
- carbazole compound
- banana
- indole carbazole
- vascular wilt
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides application of the indole carbazole compound in preparing resisting banana vascular wilt cause of disease microbial inoculum.In the structure of the indole carbazole compound such as Formulas I to formula IV shown in any compound.The present invention technique effect be:The present invention has developed a kind of high-efficiency low-toxicity natural pesticide, inhibited to the growth of Pathogen Causing Banana Fusarium Wilt Fusarium oxysporum No. 4 biological strains of f.sp.cubense, has certain preventive and therapeutic effect to banana blight.
Description
Technical field
The present invention relates to natural medicine technical field, more particularly to indole carbazole compound is preparing resisting banana vascular wilt
Application in cause of disease microbial inoculum.
Background technology
Banana is liked as a kind of tropical fruit (tree) with a variety of medicinal efficacies by people, makees as four generalized grain of the world
One of object, importance are only second to rice, wheat and maize.Banana is the maximum application kind in Hainan Province, while being sea
South saves the most important pillar of the economy of agricultural production, plays an important role in Hainan Province's economic development.However, banana is withered, one
The crushing soil of kind passes micro-bundle disease, since Panama is greatly, has given the main banana such as Asia, South America and Africa within 1910
Plantation area causes huge loss.The disease pathogen (Fusarium oxysporum f.sp.cubense) has 4 lifes
Microspecies are managed, wherein it is No. 1 and No. 4 microspecies to endanger maximum, No. 1 microspecies main harm dwarf banana veriety, and No. 4 microspecies are almost endangered
The all types of banana varieties of evil.In 20th century 60, the seventies, the Musa AAB on the ground such as China Guangdong, Guangxi, Fujian and Yunnan and
Dwarf banana large area infects No. 1 microspecies, and this time morbidity causes destructive loss;It is found in China Guangdong Panyu district, China within 1996
No. 4 microspecies, later 2001 soon its found in Sanya, Hainan, main harm Brazil any of several broadleaf plants, at present the germ harm expanded
Hainan Province multiple cities and counties' banana plantings base is arrived greatly, and morbidity any of several broadleaf plants garden diseased plant rate reaches 30%-50%, serious to cause up to 90% or more
Jiao Yuan leaves uncultivated, and therefore, the prevention of banana blight has become Hainan Province's banana industry and is badly in need of the major issue solved.
In the method for numerous prevention banana blights, chemical method is most direct, effective and convenient, and banana is withered
Disease is a kind of soil-borne vascular bundle disease, and pathogen infects sprawling after being invaded by root, basal part of stem in vascular bundle, general chemistry
Medicament is difficult to play a role.It is directed in the correlative study that the pathogen carries out both at home and abroad in recent years, not screening obtains very
Effective chemical agent.Now on the market in addition to carbendazim and phenylpropyl alcohol thiazole to its with certain preventive and therapeutic effect other than, other sterilizations
Agent is without effect.However, a large amount of frequent uses of such pesticide so that pathogen gradually develops immunity to drugs to it, usually makes
Prevention failure, and pollute the environment, but also Residual Pesticides in Farm Produce, influences the health of people, in agricultural
In terms of foreign exchange earning, the competitiveness of China's agricultural and sideline product is reduced.Therefore, new high activity, the natural agriculture of hypotoxicity are found
Medicine is extremely urgent for preventing banana blight, has important practical significance.
Invention content
In view of this, the answering in preparing resisting banana vascular wilt cause of disease microbial inoculum the present invention provides indole carbazole compound
With.The indole carbazole compound is to Pathogen Causing Banana Fusarium Wilt Fusarium oxysporum f.sp.cubense 4
The growth of biological strain is inhibited.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme:
The present invention provides application of the indole carbazole compound in preparing resisting banana vascular wilt cause of disease microbial inoculum.
Preferably, indole carbazole compound is any compound in Formulas I to formula IV:
Preferably, Pathogen Causing Banana Fusarium Wilt is No. 4 microspecies of Fusarium oxysporum f.sp.Cubense.
Preferably, resisting banana vascular wilt cause of disease microbial inoculum further includes acceptable auxiliary agent in Pesticide Science.
Preferably, resisting banana vascular wilt cause of disease microbial inoculum further includes the drug of other resisting banana vascular wilt pathogens.
Preferably, the dosage form of resisting banana vascular wilt cause of disease microbial inoculum is wettable powder, water dispersible granules, suspending agent or breast
Oil.
Preferably, the weight percent that indole carbazole compound accounts for the resisting banana vascular wilt cause of disease microbial inoculum is 1%
~99%.
Preferably, indole carbazole compound account for the resisting banana vascular wilt cause of disease microbial inoculum weight percent be 10%~
90%.
The present invention provides application of the indole carbazole compound in preparing resisting banana vascular wilt cause of disease microbial inoculum.The indoles
In the structure of carbazole compound such as Formulas I to formula IV shown in any compound.The present invention technique effect be:The present invention researches and develops
A kind of high-efficiency low-toxicity natural pesticide, to Pathogen Causing Banana Fusarium Wilt Fusarium oxysporum f.sp.cubense 4
The growth of biological strain is inhibited, has certain preventive and therapeutic effect to banana blight.
Specific implementation mode
The invention discloses application of the indole carbazole compound in preparing resisting banana vascular wilt cause of disease microbial inoculum, this fields
Technical staff can use for reference present disclosure, be suitably modified technological parameter realization.In particular, it should be pointed out that all similar replacements
Apparent to those skilled in the art with changing, they are considered as being included in the present invention.The method of the present invention
And application is described by preferred embodiment, related personnel can obviously not depart from the content of present invention, spirit and model
Enclose it is interior to method described herein and application be modified or suitably change and combine, to realize and apply the technology of the present invention.
PDB culture mediums:Potato-dextrose broth, Potato-dextrose broth bouillon are formulated and are:
200 grams of potato, 20 grams of glucose, ddH21000 milliliters of O, nature pH.
Indole carbazole compound provided by the invention is used in the application in preparing resisting banana vascular wilt cause of disease microbial inoculum
Bulk pharmaceutical chemicals or auxiliary material are available on the market.
With reference to embodiment, the present invention is further explained:
1 indole carbazole compound of embodiment is to No. 4 biological strains of Fusarium oxysporum f.sp.cubense
Inhibiting effect
1. prepared by bacterium solution:It prepares PDB culture mediums 100mL to be placed in 250mL triangle shake bottles, is engaged in the withered with banana of activation
Wither on the PDA plate of germ (Fusarium oxysporum f.sp.cubense) No. 4 microspecies the big small bacteria block of the yellowish green beans of picking in
In PDB culture mediums, it is placed in shaking table with 120r/min, 28 DEG C of cultures are for 24 hours.Then the sterile PDB of cultured bacterium solution is cultivated
Base is diluted to 5 × 105CFU (blood counting chamber counting).
2. prepared by sample solution:Monomeric compound to be measured and positive control drug (carbendazim) are formulated as by solvent of DMSO
The solution for later use of a concentration of 20mg/mL.
3. concrete operations:The sterile PDB culture mediums of 190 μ L are added in the first row of 96 orifice plates, 100 μ L are added in other holes
Sterile PDB culture mediums are separately added into 10 μ L sample solution in first row, 100 μ L are sucked out after being beaten with liquid-transfering gun in secondary series,
Be sucked out 100 μ L solution in next column, last row is sucked out 100 μ L solution and discards after successively sample being beaten according to above method.So
Afterwards plus 100 μ L of ready bacterium solution are in each hole.Each sample is repeated 3 times.96 orifice plates handled well are placed in 28 DEG C of incubators
Result is observed in middle culture afterwards for 24 hours.Be judged as with liquid-transparent it is antibacterial, drug minimum concentration asepsis growth person be minimum inhibitory concentration
(MIC)。
1 indole carbazole compound of table to No. 4 biological strains of Fusarium oxysporum f.sp.cubense most
Small inhibition concentration
Compound | MIC(μM) |
1 | 90.5 |
2 | 105.0 |
3 | 50.5 |
4 | 77.1 |
Carbendazim | 20.5 |
The results are shown in Table 1, and 4 kinds of indole carbazole compounds are to Fusarium oxysporum f.sp.cubense 4
The growth of number biological strain has certain inhibiting effect.
2 acute toxicity testing of embodiment
Kunming mouse, gavage is taken to give indole carbazole compound provided by the invention, given low 1g/kg.
Above-mentioned indole carbazole compound is taken respectively, then continuous gavage 4 times in 1 day give normal drink per minor tick 6h
Food, is observed continuously 14 days, carries out pathologic inspection to each organ, does not find organ disease.
3 long term toxicity test of embodiment
Kunming mouse, gavage is taken to give indole carbazole compound provided by the invention, middle dosage gavage processing group fills
Stomach dosage is 1.6g/kg;Large dosage of gavage processing group given low is 2.5g/kg.
Above-mentioned indole carbazole compound, daily gavage 2 times, continuous gavage is taken to measure mouse weight, the heart after 14 days respectively
The indexs such as function, liver function, renal function, electrocardiogram, gavage group compared with the control group, are shown no obvious abnormalities respectively.Pathology are examined
Look into the internal organs such as the heart, liver,kidney,spleen, lung, Stomach duodenum, large intestine, small intestine, adrenal gland and genitals, gavage group respectively with compare
Group compares, and is showed no apparent poisoning and sexually revises.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
1. application of the indole carbazole compound in preparing resisting banana vascular wilt cause of disease microbial inoculum.
2. application according to claim 1, which is characterized in that the indole carbazole compound is to appoint in Formulas I to formula IV
A kind of compound:
3. application according to claim 1, which is characterized in that the Pathogen Causing Banana Fusarium Wilt is Fusarium
No. 4 microspecies of oxysporum f.sp.Cubense.
4. application according to claim 1, which is characterized in that the resisting banana vascular wilt cause of disease microbial inoculum further includes Pesticide Science
Upper acceptable auxiliary agent.
5. application according to claim 1, which is characterized in that the resisting banana vascular wilt cause of disease microbial inoculum further includes others
The drug of resisting banana vascular wilt pathogen.
6. application according to claim 1, which is characterized in that the dosage form of the resisting banana vascular wilt cause of disease microbial inoculum is wettable
Property pulvis, water dispersible granules, suspending agent or missible oil.
7. application according to claim 1, which is characterized in that it is withered that the indole carbazole compound accounts for the anti-banana
The weight percent of cause of disease microbial inoculum is 1%~99%.
8. application according to any one of claim 1 to 7, which is characterized in that the indole carbazole compound accounts for institute
The weight percent for stating resisting banana vascular wilt cause of disease microbial inoculum is 10%~90%.
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20050072341A (en) * | 2004-01-06 | 2005-07-11 | 학교법인고려중앙학원 | Streptomyces roseoflavus ls-a24 strain effective against plant pathogenic fungi and fungicides produced from it |
CN102925511A (en) * | 2012-06-19 | 2013-02-13 | 文才艺 | Staurosporine preparation method |
WO2013100655A1 (en) * | 2011-12-27 | 2013-07-04 | 대한민국(농촌진흥청장) | Composition for controlling bacterial leaf blight of rice |
KR20170054791A (en) * | 2015-11-10 | 2017-05-18 | 대한민국(농촌진흥청장) | Composition containing K252d derived from Streptomyces for rice bacterial blight suppressing activity |
-
2018
- 2018-03-07 CN CN201810185161.0A patent/CN108402057A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20050072341A (en) * | 2004-01-06 | 2005-07-11 | 학교법인고려중앙학원 | Streptomyces roseoflavus ls-a24 strain effective against plant pathogenic fungi and fungicides produced from it |
WO2013100655A1 (en) * | 2011-12-27 | 2013-07-04 | 대한민국(농촌진흥청장) | Composition for controlling bacterial leaf blight of rice |
CN102925511A (en) * | 2012-06-19 | 2013-02-13 | 文才艺 | Staurosporine preparation method |
KR20170054791A (en) * | 2015-11-10 | 2017-05-18 | 대한민국(농촌진흥청장) | Composition containing K252d derived from Streptomyces for rice bacterial blight suppressing activity |
Non-Patent Citations (4)
Title |
---|
HEE JIN PARK ET.AL.: "Isolation and Antifungal and Antioomycete Activities of Staurosporine from Streptomyces roseoflavus Strain LS-A24", 《J. AGRIC. FOOD CHEM.》 * |
J. T. LINK ET.AL.: "First Total Synthesis of Staurosporine and enf-Staurosporine", 《J. AM. CHEM. SOC.》 * |
李敏慧等: "香蕉枯萎病菌1号和4号生理小种的快速检测与鉴定", 《中国农业科学》 * |
高春燕等: "拟诺卡菌及其次级代谢产物", 《中国抗生素杂志》 * |
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Application publication date: 20180817 |