CN108384738A - One Pseudomonas aeruginosa strain and its screening technique and its application in stalk lactic fermentation - Google Patents
One Pseudomonas aeruginosa strain and its screening technique and its application in stalk lactic fermentation Download PDFInfo
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Abstract
The present invention provides a Pseudomonas aeruginosa strain, deposit number is CCTCC NO：M2018169；The present invention also provides the screening technique of the Pseudomonas aeruginosa strain and its applications in stalk lactic fermentation；The Pseudomonas aeruginosa strain is to screen to obtain from the aerobic tank activated sludge of municipal sewage plant, it can a variety of typical products for being generated in preprocessing process of high-efficiency lignin degrading, it is cultivated 36 hours under the conditions of 37 DEG C, 100% is reached to the degradation rate of each substrate coumaric acid, parahydroxyben-zaldehyde, vanillic aldehyde, ferulic acid, syringaldehyde；Stalk lactic fermentation is carried out using the Pseudomonas aeruginosa strain, it can avoid microbial metabolism when these typical products inhibit stalk lactic fermentation, to improve lactic acid to stover rate, and fermentation period is short, 48 72h then fermentation ends are cultivated, fermentation raw material is done using straw, turns waste into wealth, environmental pollution is reduced, to ensureing that grain security has positive effect.
Description
Technical field
The present invention relates to microbiology and stalk lactic fermentation field more particularly to pseudomonas aeruginosa and its screening sides
The application of method and pseudomonas aeruginosa in stalk lactic fermentation.
Background technology
China is large agricultural country, and the yield of annual agricultural crop straw is more than 800,000,000 tons.Agricultural crop straw is mainly used as also
Field, ensiling and Huang store feed, biogas, charcoal, biomass fuel and bioenergy etc., but straw directly returning to field is decomposed
Slowly, unobvious are improved to farmland quality；As ensiling or yellow storage feed, the production cycle is long, and feed conversion rate is not high；Stalk fermentation
Production biogas will produce a large amount of biogas slurries and biogas residue, and a large amount of burnings of stalk lead to serious haze.To sum up, agricultural crop straw provides
Source is largely utilized, but still have utilization rate it is low, the problems such as secondary pollution.
It converts agricultural straw resource to the products such as lactic acid by microbial fermentation, is to realize that stalk resource efficiently utilizes
A kind of new way.Lactic acid is a kind of hydroxycarboxylic acid, is widely used in food service industry (preservative, flavoring agent), pharmaceuticals industry is (soft
Cream, lotion, injection) and leather industry (acidulant, calcium remover).Be widely used as in food industry acid, preservative and
Reducing agent.
The industrial production of lactic acid is based on microbial fermentation, and using cereal crops such as corns as raw material, this results in lactic acid valence
Lattice are high, while constituting potential threaten to national food security.Cellulose, hemicellulose in the main component of stalk
It can be converted into hexose and pentose, the homofermentative lactic process of microorganism, theoretical yield of the lactic acid to sugar by enzymolysis
It is 100%.So producing lactic acid by raw material of agricultural crop straw, the efficient utilization of stalk resource both may be implemented, improved stalk
Economic value, and can be that new approaches are opened up in the production of lactic acid, more cheap raw material extensively is provided, reduce production of lactic acid at
This, expands the application field of lactic acid, kills two birds with one stone.
Documents 1：Application No. is " CN201010609278.0 ", entitled " a kind of method for fermenting lactic acid bacteria " it is special
Profit, the invention produce lactic acid using after plant straw fibers element Partial digestion as lactobacillus-fermented carbon source through fermentation, wherein institute
A concentration of 1wt%~10wt% of sugar after the plant straw fibers element Partial digestion stated, the Partial digestion is by plant straw
Stalk fiber element, with cellulase degradation 12~72 hours, makes not only to have contained enzymolysis sugar in zymotic fluid, but also contain non-enzyme at 45~55 DEG C
The cellulose of solution, so as to the fermenting lactic acid in enzymolysis.Compared with the existing technology the production cycle was shortened to by 136 hours
64 hours, inhibiting effect of the high concentration glucose to lactobacillus-fermented lactic acid is relieved, fermentation level is increased to by 3.6wt%
6.8wt%.Fermentation raw material is done using straw, is turned waste into wealth, environmental pollution is reduced, to ensureing that grain security has actively meaning
Justice.However the invention lactic acid is not still high to stover rate, agricultural crop straw needs to be utilized by microorganism by pretreatment,
And stalk will produce a variety of by-products in preprocessing process, it is lignin and half to inhibit the metabolism of microorganism, main source
Decomposition of cellulose, including small molecular organic acid class, furans and phenols etc..Most of microbe cannot all utilize lignin,
And lignin and its Partial digestion product during the fermentation can adsorptive cellulose enzyme, formed co-precipitation, so that its activity is reduced to
It loses；Lignin, which decomposes, generates the phenolic compounds such as ferulic acid, coniferyl aldehyde, improves the mobility of microbial cell film, leads to potassium
Ion is largely lost, and is destroyed cell membrane, is inhibited the metabolism of microorganism, to influence lactic acid to stover rate.
Invention content
It is an object of the invention to overcome the defect of the prior art, a Pseudomonas aeruginosa strain and its screening technique are provided
With its application in stalk lactic fermentation, which is the aerobic tank activated sludge from municipal sewage plant
Middle screening obtains, can a variety of typical products for being generated in preprocessing process of high-efficiency lignin degrading, avoid these typical products
Inhibit microbial metabolism when stalk lactic fermentation, to improve lactic acid to stover rate, and fermentation period is short, utilizes plant
Stalk does fermentation raw material, turns waste into wealth, and reduces environmental pollution, to ensureing that grain security has positive effect.
One of the objects of the present invention is to provide a Pseudomonas aeruginosa strain, pseudomonas aeruginosa of the invention
(Bacillus subtilis), is named as pseudomonas aeruginosa FL02, from the aerobic tank activated sludge of municipal sewage plant
Middle screening obtains.
The 16S rRNA sequences such as SEQ ID NO of bacterial strain FL02:Shown in 1, primer 2 7F (5'-AGAGTT are utilized
) and the 16S of 1492R (5'-TAC GGCTACCTTGTTACGACTT-3') through the PCR amplification bacterial strain TGATCCTGGCTCAG-3'
RRNA genes.By 16S rRNA sequences in GenBank sequence analysis and tetraploid rice, pass through and compare bacterial strain FL02 and verdigris
The similitude of pseudomonad is 99%.In short, by the Morphological Identification of bacterial strain, bio-chemical characteristics and 16S rRNA sequences
Analysis and tetraploid rice, it is thus determined that bacterial strain pseudomonas aeruginosa FL02 is pseudomonas aeruginosa.
Based on information above, bacterial strain FL02 is accredited as pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterium
It has been preserved in China typical culture collection center (CCTCC), address：No. 299 forces of Wuhan City, Hubei Province Wuchang District Bayi Road
In Chinese institution of higher education, Wuhan University's collection, postcode：430072, deposit number CCTCCNO：M2018169, preservation date 2018
On March 30, in.
The second object of the present invention is to provide the screening technique of the Pseudomonas aeruginosa strain comprising following steps：
Enriched medium is added in step 1 in culture bottle, sequentially adds water treatment plant's secondary settling tank sludge, printing and dyeing mill goes out
Soil near the mouth of a river, obtains enrichment culture liquid, the enriched medium includes vanillic aldehyde, para hydroxybenzene after shake culture after sealing
Formaldehyde, syringaldehyde and yeast extract；
Step 2 installs screening and culturing medium in culture bottle, and the enrichment culture liquid that the step 1 obtains is added, is shaken after sealing
Swing culture, obtain screening seed liquor, the screening and culturing medium include vanillic aldehyde, parahydroxyben-zaldehyde, syringaldehyde, guaiacol,
Ferulic acid, coumaric acid, yeast extract, MgSO4 7H2O、CaCl2、(NH4)2SO4、K2HPO4, NaCl and FeCl3；
Step 3, by the screening seed liquor obtained by step 2 after gradient dilution, be coated on solid screening and culturing medium tablet
On, after stationary culture, single bacterium colony that picking has been grown；
Step 4 by single bacterium colony preservation that picking in step 3 obtains and does 16S rDNA identifications, and qualification result is that verdigris is false
Monad.
The third object of the present invention is to provide application of the Pseudomonas aeruginosa strain in stalk lactic fermentation.
Specific implementation mode as application of the Pseudomonas aeruginosa strain in stalk lactic fermentation comprising following step
Suddenly：
Step S1, maize straw pre-processes：Maize straw is weighed in aluminium foil bag, concentrated ammonia liquor is added, it is rapid to seal, repeatedly
Rubbing aluminium foil bag makes NH3It comes into full contact with, is placed three days or more under room temperature, you can obtain ammonia treatment stalk with stalk；
Step S2, stalk lactic fermentation：The pretreatment maize straw that the step S1 is obtained is filled in culture bottle, is connect simultaneously
Pseudomonas aeruginosa described in kind condensation pseudomonas aeruginosa and right 1, addition complex cellulase is as substrate, standing for fermentation
Up to lactic acid finished product after culture.
The invention has the advantages that：
1, the Pseudomonas aeruginosa strain provided by the invention can high-efficiency lignin degrading generated in preprocessing process it is more
Kind of typical product, such as parahydroxyben-zaldehyde, coumaric acid, vanillic aldehyde, ferulic acid, syringaldehyde, pseudomonas aeruginosa FL02 can more than
It is sole carbon source to state compound, and 150r/min is cultivated 36 hours under the conditions of 37 DEG C, to each substrate coumaric acid, parahydroxyben-zaldehyde,
Vanillic aldehyde, ferulic acid, syringaldehyde degradation rate reach 100%.
2, the Pseudomonas aeruginosa strain provided by the invention can high-efficiency lignin degrading generated in preprocessing process it is more
Kind typical product, microbial metabolism when these typical products being avoided to inhibit stalk lactic fermentation, to improve lactic acid to stalk
Conversion ratio, lactic acid is up to 65% to stover rate in stalk lactic fermentation experiment；
3, application of the Pseudomonas aeruginosa strain provided by the invention in stalk lactic fermentation, fermentation period is short, 37 DEG C
Under the conditions of cultivate 36 hours can degradable a variety of typical products, culture 48-72h then fermentation ends；
4, the present invention does fermentation raw material using straw, turns waste into wealth, and reduces environmental pollution, to ensureing grain security tool
There is positive effect.
The deposit date is on March 30th, 2018, deposit number CCTCCNO for the bacterial strain of the present invention：M2018169, classification life
Entitled Pseudomonas aeruginosa, the entitled China typical culture collection center of depositary institution (CCTCC), address is
No. 299 Wuhan Universitys of Wuhan City, Hubei Province Wuchang District Bayi Road are in the school.
Specific implementation mode
The screening of 1 bacterial strain FL02 of embodiment
1,90ml enriched mediums (1g/L vanillic aldehyde+1g/L parahydroxyben-zaldehydes+1g/L is added in 250ml triangular flasks
Syringaldehyde+1g/L yeast extracts, pH7.0), then it is separately added into water treatment plant's secondary settling tank sludge, printing and dyeing mill's water outlet soil nearby
Each 10g, sealing are placed on 37 DEG C of shaking tables, and 150r/min shake cultures for 24 hours, obtain enrichment culture liquid.
2,100ml screening and culturing medium (1g/L vanillic aldehyde+1g/L parahydroxyben-zaldehyde+1g/L fourths are filled in 250ml triangular flasks
Fragrant aldehyde+1g/L guaiacol+1g/L ferulic acids+1g/L coumaric acid+0.5g/L yeast extract+0.2g/L MgSO4 7H2O,0.1g/
L CaCl2,1g/L(NH4)2SO4,1g/L K2HPO4,0.1g/L NaCl,0.02g/L FeCl3, pH7.0), it is separately added into 1ml
Enrichment culture liquid, sealing are placed on 37 DEG C of shaking tables, and 150r/min shake culture 48h obtain screening seed liquor.
3, seed liquor will be screened after gradient dilution, be coated on solid screening and culturing medium tablet, 37 DEG C of stationary cultures
48h, picking single bacterium colony, you can obtain bacterial strain FL02.
The identification of embodiment 2, bacterial strain FL02
The 16S rRNA sequences such as SEQ ID NO of bacterial strain FL02:Shown in 1, primer 2 7F (5'- are utilized
AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TAC GGCTACCTTGTTACGACTT-3') is through the PCR amplification bacterial strain
16S rRNA genes.By 16S rRNA sequences in GenBank sequence analysis and tetraploid rice, pass through compare bacterial strain FL02
Similitude with Pseudomonas aeruginosa is 99%.Bacterial strain FL02 gives birth to by Morphological Identification, the physiology to bacterial strain
Change experiment and 16S rRNA sequence analyses and tetraploid rice, by comparing bacterial strain FL02 and Pseudomonas aeruginosa
Similitude be 99%, it is thus determined that bacterial strain pseudomonas aeruginosa FL02 be pseudomonas aeruginosa.
The microbial characteristic of bacterial strain FL02 is as follows：Thalline is elongated and different in size, sometimes in club-shaped or threadiness, in pairs
Or short catenation.There is single flagellum in one end of thalline, and visible bacteria motility is observed under dark-field microscope or phase contrast microscope
Vivaciously, thalline is elongated and different in size, is in club-shaped or threadiness, pairs of or short catenation sometimes.There is single whip in one end of thalline
Hair, no brood cell, no pod membrane.Formed on blood plate it is big and flat, moisten, have metallic luster, blue-green, transparent zone of hemolysis bacterium
It falls, have ginger taste.
The physicochemical characteristics of bacterial strain FL02 are as follows：Gram-negative bacteria, oxidase positive produce pyocyanin, and agar is dyed yellowish green
Color, it is specific as shown in table 1.
Table 1
The degradation of embodiment 3, bacterial strain FL02 p-Coumaric Acids, parahydroxyben-zaldehyde, vanillic aldehyde, ferulic acid, syringaldehyde
Pseudomonas aeruginosa strains (Pseudomonas aeruginosa) FL02 that detection present invention screening obtains is to perfume (or spice)
Beans acid, parahydroxyben-zaldehyde, vanillic aldehyde, ferulic acid, the degradation of syringaldehyde or utilization obstacle.
Specific experiment process is as follows：The pseudomonas aeruginosa strains for taking 5 parts of equivalent present invention to screen
(Pseudomonas aeruginosa) FL02 is added separately in culture medium 1-5, and the culture medium 1-5 contains 0.5g/L ferment
Mother's leaching powder, 0.2g/L MgSO4.7H2O,0.1g/L CaCl2,1g/L(NH4)2SO4,1g/L K2HPO4,0.1g/L NaCl,
0.02g/L FeCl3, and contain 1g/L vanillic aldehydes, 1g/L parahydroxyben-zaldehydes, 1g/L syringaldehydes, 1g/ in culture medium 1-5 respectively
L coumaric acids, 1g/L ferulic acids, pseudomonas aeruginosa FL02 can the above compound be sole carbon source, 150r/min, 37 DEG C of conditions
Lower culture 36 hours, the results show that pseudomonas aeruginosa strains (Pseudomonas aeruginosa) FL02 can be with above-mentioned
Substrate is carbon source for growth.In addition, being cultivated by 36h, as a result each substrate content of sample detection shows pseudomonas aeruginosa strains
(Pseudomonas aeruginosa) FL02 is degradable to each substrate, to each substrate coumaric acid, parahydroxyben-zaldehyde, chinese cymbidium
Element, ferulic acid, syringaldehyde degradation rate reach 100%.
Conversion ratio of 4 stalk lactic fermentation of the embodiment experiment detection lactic acid to stalk
1, maize straw pretreatment method：Maize straw 80g is weighed in aluminium foil bag, be added volumetric concentration be 25% it is dense
Ammonium hydroxide 20ml, it is rapid to seal, aluminium foil bag is rubbed repeatedly, makes NH3It comes into full contact with stalk, is placed three days or more under room temperature, you can
To ammonia treatment stalk.
2, stalk lactic fermentation is tested：Take two parts of pretreatments for filling a concentration of 50g/L of 100ml in 250ml triangular flasks beautiful
Rice stalk, while being inoculated with 1ml and condensing pseudomonas aeruginosa LA209 and 1ml pseudomonas aeruginosa FL02 as experimental group, and only connect
As a control group, experimental group and control group add complex cellulase 10FPU/g to kind 1ml condensation pseudomonas aeruginosas LA209
(substrate).50 DEG C of standing for fermentation, per 12h, adjusting pH is primary, controls in 6.0-6.5.After culture 48-72h or pH no longer becomes
Change, then fermentation ends.The lactic acid, acetic acid, total phenols content of each experimental group are detected, calculates conversion ratio, experimental group and control group are equal
3 repeated experiments.Such as the following table 2.
Table 2
It is above-mentioned the experimental results showed that, compared to control group, the lactic acid concn of experimental group 1-3 is significantly increased, and inhibits micro- life
The yield of the product such as acetic acid and total phenol of object metabolism etc., microorganism generation when these typical products being avoided to inhibit stalk lactic fermentation
It thanks, to improve lactic acid to stover rate, lactic acid is up to 65% to stover rate in stalk lactic fermentation experiment.
Application of the Pseudomonas aeruginosa strain provided by the invention in stalk lactic fermentation, fermentation period is short, 37 DEG C of items
Cultivated under part 36 hours can degradable a variety of typical products, culture 48-72h then fermentation ends；The present invention utilizes plant
Stalk does fermentation raw material, turns waste into wealth, and reduces environmental pollution, to ensureing that grain security has positive effect.
The deposit number of condensation pseudomonas aeruginosa LA209 in its Chinese：CCTCC No.2015773, specific visible Shen
Please number be " CN201610044993.1 ", it is entitled " bacillus coagulans and its cultural method with prepare answering for biological organic fertilizer
With " patent.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Sequence table
<110>Hua Zhong Agriculture University
<120>One Pseudomonas aeruginosa strain and its screening technique and its application in stalk lactic fermentation
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1387
<212> DNA
<213>Pseudomonas aeruginosa (Pseudomonas aeruginosa)
<400> 1
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ggaatctgcc tggtagtggg ggataacgtc cggaaacggg cgctaatacc gcatacgtcc 120
tgagggagaa agtgggggat cttcggacct cacgctatca gatgagccta ggtcggatta 180
gctagttggt ggggtaaagg cctaccaagg cgacgatccg taactggtct gagaggatga 240
tcagtcacac tggaactgag acacggtcca gactcctacg ggaggcagca gtggggaata 300
ttggacaatg ggcgaaagcc tgatccagcc atgccgcgtg tgtgaagaag gtcttcggat 360
tgtaaagcac tttaagttgg gaggaagggc agtaagttaa taccttgctg ttttgacgtt 420
accaacagaa taagcaccgg ctaacttcgt gccagcagcc gcggtaatac gaagggtgca 480
agcgttaatc ggaattactg ggcgtaaagc gcgcgtaggt ggttcagcaa gttggatgtg 540
aaatccccgg gctcaacctg ggaactgcat ccaaaactac tgagctagag tacggtagag 600
ggtggtggaa tttcctgtgt agcggtgaaa tgcgtagata taggaaggaa caccagtggc 660
gaaggcgacc acctggactg atactgacac tgaggtgcga aagcgtgggg agcaaacagg 720
attagatacc ctggtagtcc acgccgtaaa cgatgtcgac tagccgttgg gatccttgag 780
atcttagtgg cgcagctaac gcgataagtc gaccgcctgg ggagtacggc cgcaaggtta 840
aaactcaaat gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag 900
caacgcgaag aaccttacct ggccttgaca tgctgagaac tttccagaga tggattggtg 960
ccttcgggaa ctcagacaca ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt 1020
gggttaagtc ccgtaacgag cgcaaccctt gtccttagtt accagcacct cgggtgggca 1080
ctctaaggag actgccggtg acaaaccgga ggaaggtggg gatgacgtca agtcatcatg 1140
gcccttacgg ccagggctac acacgtgcta caatggtcgg tacaaagggt tgccaagccg 1200
cgaggtggag ctaatcccat aaaaccgatc gtagtccgga tcgcagtctg caactcgact 1260
gcgtgaagtc ggaatcgcta gtaatcgtga atcagaatgt cacggtgaat acgttcccgg 1320
gccttgtaca caccgcccgt cacaccatgg gagtgggttg ctccagaagt agctagtcta 1380
accgcaa 1387
Claims (10)
1. a Pseudomonas aeruginosa strain, which is characterized in that it is named as pseudomonas aeruginosa FL02, and deposit number is
CCTCCNO：M2018169.
2. a kind of screening technique of pseudomonas aeruginosa described in claim 1, which is characterized in that it includes the following steps：
Enriched medium is added in step 1 in culture bottle, sequentially adds water treatment plant's secondary settling tank sludge, printing and dyeing mill's water outlet
Neighbouring soil, obtains enrichment culture liquid after shake culture after sealing, the enriched medium includes vanillic aldehyde, para hydroxybenzene first
Aldehyde, syringaldehyde and yeast extract；
Step 2 installs screening and culturing medium in culture bottle, and the enrichment culture liquid that the step 1 obtains is added, and training is shaken after sealing
It supports, obtains screening seed liquor, the screening and culturing medium includes vanillic aldehyde, parahydroxyben-zaldehyde, syringaldehyde, guaiacol, asafoetide
Acid, coumaric acid, yeast extract, MgSO47H2O、CaCl2、(NH4)2SO4、K2HPO4, NaCl and FeCl3；
Step 3, by the screening seed liquor obtained by step 2 after gradient dilution, be coated on solid screening and culturing medium tablet, it is quiet
After setting culture, single bacterium colony that picking has been grown；
Step 4 by single bacterium colony preservation that picking in step 3 obtains and does 16S rDNA identifications, and qualification result is P. aeruginosa
Bacterium.
3. the screening technique of pseudomonas aeruginosa as claimed in claim 2, which is characterized in that the enrichment culture based formulas is
1g/L vanillic aldehyde+1g/L parahydroxyben-zaldehyde+1g/L syringaldehyde+1g/L yeast extracts, pH7.0.
4. the screening technique of pseudomonas aeruginosa as claimed in claim 2, which is characterized in that the formula of the screening and culturing medium
For 1g/L vanillic aldehyde+1g/L parahydroxyben-zaldehyde+1g/L syringaldehyde+1g/L guaiacol+1g/L ferulic acid+1g/L coumaric acids+
0.5g/L yeast extract+0.2g/L MgSO47H2O+0.1g/L CaCl2,1g/L(NH4)2SO4+1g/LK2HPO4+0.1g/LNaCl
+0.02g/L FeCl3, pH7.0.
5. the screening technique of pseudomonas aeruginosa as claimed in claim 2, which is characterized in that in the step 1, in 250ml
90ml enriched mediums are added in triangular flask, sequentially add water treatment plant's secondary settling tank sludge, printing and dyeing mill's water outlet soil nearby
Each 10g, sealing are placed on 37 DEG C of shaking tables, and 150r/min shake cultures for 24 hours, obtain enrichment culture liquid.
6. the screening technique of pseudomonas aeruginosa as claimed in claim 2, which is characterized in that in the step 2, in 250ml
100ml screening and culturing mediums are filled in triangular flask, and the 1ml enrichment culture liquid obtained by step 1 is added, sealing is placed on 37 DEG C of shaking tables,
150r/min shake culture 48h obtain screening seed liquor；In the step 3, by screening seed liquor after gradient dilution, apply
It is distributed on solid screening and culturing medium tablet, 37 DEG C of stationary culture 48h, picking single bacterium colony.
7. application of the pseudomonas aeruginosa described in claim 1 in stalk lactic fermentation.
8. application of the pseudomonas aeruginosa as claimed in claim 7 in stalk lactic fermentation, which is characterized in that including following
Step：
Step S1, maize straw pre-processes：Maize straw is weighed in aluminium foil bag, concentrated ammonia liquor is added, it is rapid to seal, aluminium is rubbed repeatedly
Foil bag makes NH3It comes into full contact with, is placed three days or more under room temperature, you can obtain ammonia treatment stalk with stalk；
Step S2, stalk lactic fermentation：The pretreatment maize straw that the step S1 is obtained is filled in culture bottle, while being inoculated with solidifying
The pseudomonas aeruginosa described in pseudomonas aeruginosa and right 1 is tied, addition complex cellulase is as substrate, standing for fermentation culture
Afterwards up to lactic acid finished product.
9. application of the pseudomonas aeruginosa as claimed in claim 8 in stalk lactic fermentation, which is characterized in that the step
In S1, maize straw 80g is weighed in aluminium foil bag, and the concentrated ammonia liquor 20ml that volumetric concentration is 25% is added.
10. application of the pseudomonas aeruginosa as claimed in claim 8 in stalk lactic fermentation, which is characterized in that the step
In rapid S2, the pretreatment maize straw of a concentration of 50g/L of 100ml is filled in 250ml triangular flasks, while being inoculated with 1ml condensation verdigris
Pseudomonad LA209 and 1ml pseudomonas aeruginosa FL02, addition complex cellulase 10FPU/g (substrate), 50 DEG C stand hair
Ferment, adjusts that pH is primary, and pH is in 6.0-6.5 for control per 12h, cultivates after 84h or when pH no longer changes, then fermentation ends to obtain the final product
Lactic acid finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810399264.7A CN108384738B (en) | 2018-04-28 | 2018-04-28 | Pseudomonas aeruginosa, screening method thereof and application thereof in straw lactic acid fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810399264.7A CN108384738B (en) | 2018-04-28 | 2018-04-28 | Pseudomonas aeruginosa, screening method thereof and application thereof in straw lactic acid fermentation |
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| CN112080450A (en) * | 2020-09-30 | 2020-12-15 | 内蒙古农业大学 | Straw degrading bacteria and separation screening method |
| CN116536209A (en) * | 2023-05-15 | 2023-08-04 | 四川大学 | Pseudomonas azotoformans YF-58 for high yield of guaiacol and application thereof |
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| CN101857847A (en) * | 2010-05-06 | 2010-10-13 | 合肥工业大学 | Pseudomonas aeruginosa strain separating, purifying and domesticating method and use |
| CN106520615A (en) * | 2016-11-16 | 2017-03-22 | 河南农业大学 | Composite microbial system for quickly degrading maize straw and preparation and pretreatment method thereof |
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| US20090191612A1 (en) * | 2002-03-21 | 2009-07-30 | Council Of Scientific And Industrial Research | Biological process for color reduction of pulp and paper effluent |
| CN101857847A (en) * | 2010-05-06 | 2010-10-13 | 合肥工业大学 | Pseudomonas aeruginosa strain separating, purifying and domesticating method and use |
| CN106520615A (en) * | 2016-11-16 | 2017-03-22 | 河南农业大学 | Composite microbial system for quickly degrading maize straw and preparation and pretreatment method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112080450A (en) * | 2020-09-30 | 2020-12-15 | 内蒙古农业大学 | Straw degrading bacteria and separation screening method |
| CN112080450B (en) * | 2020-09-30 | 2022-04-19 | 内蒙古农业大学 | Straw degrading bacteria and separation screening method |
| CN116536209A (en) * | 2023-05-15 | 2023-08-04 | 四川大学 | Pseudomonas azotoformans YF-58 for high yield of guaiacol and application thereof |
| CN116536209B (en) * | 2023-05-15 | 2024-04-19 | 四川大学 | Pseudomonas azotoformans YF-58 for high yield of guaiacol and application thereof |
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