CN108382745B - A kind of small-sized biological biopsy sample container - Google Patents

A kind of small-sized biological biopsy sample container Download PDF

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Publication number
CN108382745B
CN108382745B CN201711126987.1A CN201711126987A CN108382745B CN 108382745 B CN108382745 B CN 108382745B CN 201711126987 A CN201711126987 A CN 201711126987A CN 108382745 B CN108382745 B CN 108382745B
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China
Prior art keywords
small
biopsy sample
sample container
vessel
sized biological
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CN201711126987.1A
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Chinese (zh)
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CN108382745A (en
Inventor
倪兵
王奕文
陈瑛
王丽
巩志伟
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East China Normal University
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East China Normal University
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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D85/00Containers, packaging elements or packages, specially adapted for particular articles or materials
    • B65D85/50Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D51/00Closures not otherwise provided for
    • B65D51/24Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes
    • B65D51/28Closures not otherwise provided for combined or co-operating with auxiliary devices for non-closing purposes with auxiliary containers for additional articles or materials

Abstract

The invention discloses a kind of small-sized biological biopsy sample container, including vessel and sealable lid together in the lid of the vessel;Wherein, the vessel is arranged for accommodating the biological living sample;The lid includes an accommodating chamber, and the accommodating chamber is arranged for accommodating oxygen;The accommodating chamber has a ventilating mechanism towards the vessel side;In use process, the oxygen in the accommodating chamber diffuses in the vessel from the ventilating mechanism.Small-sized biological biopsy sample container of the invention, not only carrying and convenient transportation, but also the existence for a long time of permission living body biological sample, thus can be with long-distance transportation living body biological sample.

Description

A kind of small-sized biological biopsy sample container
Technical field
The present invention relates to biology and field of ecology more particularly to a kind of small-sized biological biopsy sample containers.
Background technique
During biology and ecological study, the acquisition of living animal sample is a basic work, for life Object and ecological Studies are of great significance to.The living animal for needing to carry out sample collection includes such as insect, aquatic life Object, small-sized reptile, small-sized amphibian, small-sized mammalian etc..
Currently, only being saved it in easy container after collecting living body biological sample in field environment.Due to difficulty To build good living environment, the living body biological sample of acquisition is acquired, not only the holding time is short, the death rate is high, inconvenient to carry, And it is difficult to carry out long distance transportation.
Therefore, be badly in need of a kind of living body biological sample that can permit acquisition in this field to survive for a long time, be convenient for carrying and The living body biological sample retention devices of long-distance transportation.
Summary of the invention
In view of the above drawbacks of the prior art, the present invention provides a kind of permission living body biological samples to survive for a long time, It is convenient for carrying the living body biological sample container with long-distance transportation.
The small-sized biological biopsy sample container of offer of the invention, including vessel and sealable lid are together in the container The lid of ontology;Wherein, the vessel is arranged for accommodating the biological living sample;The lid includes a receiving Chamber, the accommodating chamber are arranged for accommodating oxygen;The accommodating chamber has a ventilating mechanism towards the vessel side; In use process, the oxygen in the accommodating chamber diffuses in the vessel from the ventilating mechanism.
Preferably, the accommodating chamber includes an elastic balloon, and the opening of the elastic balloon is connected to the ventilating mechanism.
Preferably, the ventilating mechanism includes a gas-guide tube, and oxygen arrives at the vessel by the gas-guide tube Depths.It is highly preferred that the gas-guide tube is made of hard material (such as plastics).
Preferably, the gas-guide tube includes first segment and second section, and the second section is socketed in the first segment The first segment can be retracted in section and partly or entirely.
Preferably, the gas outlet envelope of the second section has polytetrafluoroethylene film.Polytetrafluoroethylene film is permeable watertight Film, be sealed on gas outlet so that oxygen can free diffusing, and the water and biological sample in vessel will not enter lead Pipe.
Preferably, the side wall of the second section is equipped with oxygen diffusion window, and the oxygen spreads window Feng Youju tetra- Fluoride film.
Preferably, the ventilating mechanism includes a permanent flow valve, and the escape pipe of the permanent flow valve stretches to the vessel.
Preferably, the ventilating mechanism further includes an adjusting washer (disk), and the valve of the adjusting washer (disk) and the permanent flow valve is opened The periphery for being connected and being socketed in the permanent flow valve is closed, can be rotated relative to the permanent flow valve, it is i.e. adjustable to rotate the adjusting washer (disk) The size of oxygen flow.
Preferably, the sidewall periphery of the vessel is equipped with label interlayer, for placing label.
Preferably, the biological living sample container further includes absorption unit of dioxide carbon, the carbon dioxide absorption dress Inside the vessel.
Preferably, the absorption unit of dioxide carbon is integrally cylindrical, including support frame, is closely coated on support frame periphery Polytetrafluoroethylene film and the carbon-dioxide absorbent (such as calcium hydroxide) in support frame.
Preferably, the top surface periphery of the absorption unit of dioxide carbon is equipped with a cushion rubber, the outer diameter of cushion rubber and the container The internal diameter of ontology matches, and support frame as described above outer diameter is less than the internal diameter of the vessel.
Preferably, the through-hole passed through for the gas-guide tube is equipped in the middle part of the absorption unit of dioxide carbon.
Preferably, the small-sized biological biopsy sample is that (such as weight is less than 10g (more preferably weight to small-sized or microfauna Less than 5g) animal), including insect, aquatic animal, small-sized reptile, small-sized amphibian or small-sized mammalian;More Preferably, the small-sized biological biopsy sample is protozoan biopsy sample.
Technical effect
Biological living sample container of the invention, not only carry and convenient transportation, but also allow living body biological sample it is long when Between survive, effectively reduce the death rate of biopsy sample, thus different researchs can be met with long-distance transportation living body biological sample The demand of biopsy sample exchange between mechanism.
Detailed description of the invention
Fig. 1 is the overall structure diagram of small-sized biological biopsy sample container of the present invention;
Fig. 2 is the cover body structure schematic diagram of small-sized biological biopsy sample container of the present invention;
Fig. 3 is the vessel structural schematic diagram of small-sized biological biopsy sample container of the present invention;
Fig. 4 is the structural schematic diagram of the absorption unit of dioxide carbon of small-sized biological biopsy sample container of the present invention.
Specific embodiment
Embodiment 1
As shown in Figure 1, small-sized biological biopsy sample container provided in this embodiment, including vessel 1 and sealable lid Together in the lid 2 of the vessel 1;Wherein, the vessel 1 is arranged for accommodating the biological living sample;Institute Stating lid 2 includes an accommodating chamber 21, and the accommodating chamber 21 is arranged for accommodating oxygen;The accommodating chamber 21 is towards the container 1 side of ontology has a ventilating mechanism 22;In use process, the oxygen in the accommodating chamber 21 is spread from the ventilating mechanism 22 In to the vessel 1.Vessel 1 and lid 2 are connected through a screw thread in the present embodiment, between vessel 1 and lid 2 Equipped with seal washer.
In the present embodiment, the accommodating chamber 22 includes an elastic balloon 221, and the opening of the elastic balloon 221 is connected to The ventilating mechanism 22.
As shown in Fig. 2, the ventilating mechanism 22 includes a gas-guide tube 221, oxygen is arrived at described by the gas-guide tube 221 The depths of vessel 1.The gas-guide tube 221 is made of hard material (such as plastics).The gas-guide tube 221 includes first segment 222 and second section 223, the second section 223 is socketed in the first segment 222 and can partly or entirely be retracted described First segment 222.The gas outlet envelope of the second section 223 has polytetrafluoroethylene film 2231.Polytetrafluoroethylene (PTFE) in the present invention Film is the film of permeable watertight, is sealed on gas outlet, so that gas can free diffusing.Water and biology in vessel 1 Sample will not enter inside gas-guide tube 221.The side wall of the second section 223 is equipped with oxygen and spreads window 2232, the oxygen Gas diffusion 2232 envelope of window has polytetrafluoroethylene film.
The ventilating mechanism 22 includes a permanent flow valve 224, and the vessel 1 is stretched in the gas outlet of the permanent flow valve 224. In use, the first segment 222 can be tightly sleeved on the gas outlet of the permanent flow valve 224.The ventilating mechanism 22 is also Including an adjusting washer (disk) 225, the adjusting washer (disk) 225 is connected with the valve switch of the permanent flow valve 224 and is socketed in the perseverance The periphery for flowing valve 224, can rotate relative to the permanent flow valve, rotate the size of the i.e. adjustable oxygen flow of the adjusting washer (disk) 225. There were significant differences for demand of the biological living sample of different size and type to oxygen, therefore, by adjusting oxygen flow Size can control oxygen-supplying amount, and then provide suitable oxygen environment for the biopsy sample in container as much as possible.The present embodiment In, an one-way ventilating valve is set on the side wall of the vessel 1.The one-way ventilating valve is disposed in proximity to the vessel The position of 1 opening, when the air pressure in the vessel 1 is higher than atmospheric pressure, the gas in the vessel 1 can be by this One-way ventilating valve enters outside, to balance inner and outer air pressure.The poly- of one layer of waterproof and breathable is sealed in the inner wall of the one-way ventilating valve Tetrafluoroethylene.
As shown in figure 3, the sidewall periphery of the vessel 1 is equipped with label interlayer 11, for placing label.The biology Biopsy sample container further includes absorption unit of dioxide carbon 3, and the absorption unit of dioxide carbon 3 is located in the vessel 1 Portion, for absorbing the carbon dioxide that living animal breathing generates.As shown in Figure 3, Figure 4, the absorption unit of dioxide carbon 3 is whole It is cylindrical, including support frame, closely it is coated on the polytetrafluoroethylene film of support frame periphery and the carbon dioxide in support frame Absorbent (such as calcium hydroxide).The top surface periphery of the absorption unit of dioxide carbon 3 be equipped with a cushion rubber 31, the outer diameter of cushion rubber 31 and The internal diameter of the vessel 1 is equal, thus position of the absorption unit of dioxide carbon 3 inside the vessel 1 can phase To fixation, the support frame outer diameter of absorption unit of dioxide carbon 3 is less than the internal diameter of the vessel 1, can increase surface area, mention The efficiency of high-selenium corn carbon dioxide.The middle part of the absorption unit of dioxide carbon 3 is equipped with the through-hole passed through for the gas-guide tube 221 32。
Small-sized biological biopsy sample container of the invention may be reused, after the completion of each use, it is proposed that replacement dioxy Change carbon absorption device, and lid is oxygenated, when oxygenation pulls up gas-guide tube, is oxygenated from the gas outlet of permanent flow valve.
Using paramecium as small-sized biological biopsy sample, small-sized biological biopsy sample container of the invention, observation grass are used Carry out survival condition of the worm in small-sized biological biopsy sample container of the invention.
The experimental results are shown inthe following table, and lid is not oxygenated in control group, is oxygenated in experimental group, and adjusts oxygen flow and be The 1% of permanent flow valve maximum oxygen flow, theoretical oxygen release time are about 10 days, and sampling calculated paramecium survival rate from the 2nd day.
Table 1
As can be seen from the above table, biopsy sample can be greatly prolonged using small-sized biological biopsy sample container of the invention Life span, the death rate of biopsy sample is effectively reduced.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that those skilled in the art without It needs creative work according to the present invention can conceive and makes many modifications and variations.Therefore, all technologies in the art Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea Technical solution, all should be within the scope of protection determined by the claims.

Claims (9)

1. a kind of small-sized biological biopsy sample container, which is characterized in that the small-sized biological biopsy sample container includes container sheet The lid of body and sealable lid together in the vessel;Wherein, the vessel is arranged for accommodating described biological living Body sample;The lid includes an accommodating chamber, and the accommodating chamber is arranged for accommodating oxygen;The accommodating chamber is towards the appearance Device ontology side has a ventilating mechanism;In use process, the oxygen in the accommodating chamber diffuses to institute from the ventilating mechanism It states in vessel;
The accommodating chamber includes an elastic balloon, and the opening of the elastic balloon is connected to the ventilating mechanism;
The small-sized biological biopsy sample is protozoan biopsy sample;
The small-sized biological biopsy sample container further includes absorption unit of dioxide carbon, and the absorption unit of dioxide carbon is located at institute It states inside vessel;
The ventilating mechanism includes a permanent flow valve, and the escape pipe of the permanent flow valve stretches to the vessel.
2. small-sized biological biopsy sample container as described in claim 1, which is characterized in that the small-sized biological biopsy sample is Weight is less than the animal of 5g.
3. small-sized biological biopsy sample container as described in claim 1, which is characterized in that the ventilating mechanism includes an air guide Pipe, oxygen arrive at the depths of the vessel by the gas-guide tube.
4. small-sized biological biopsy sample container as claimed in claim 3, which is characterized in that the gas-guide tube includes first segment And second section, the second section are socketed in the first segment and can partly or entirely be retracted the first segment.
5. small-sized biological biopsy sample container as claimed in claim 4, which is characterized in that the gas outlet of the second section is sealed There is polytetrafluoroethylene film.
6. small-sized biological biopsy sample container as claimed in claim 5, which is characterized in that set on the side wall of the second section There is oxygen diffusion window mouth, the oxygen diffusion window envelope has polytetrafluoroethylene film.
7. small-sized biological biopsy sample container as described in claim 1, which is characterized in that the ventilating mechanism further includes a tune Washer is saved, the adjusting washer (disk) is connected with the valve switch of the permanent flow valve and is socketed in the periphery of the permanent flow valve, can be opposite The permanent flow valve rotation rotates the size of the i.e. adjustable oxygen flow of the adjusting washer (disk).
8. small-sized biological biopsy sample container as described in claim 1, which is characterized in that the absorption unit of dioxide carbon is whole Body is cylindrical, including support frame, is closely coated on the polytetrafluoroethylene film of support frame periphery and the titanium dioxide in support frame Absorbent carbon.
9. small-sized biological biopsy sample container as claimed in claim 3, which is characterized in that the gas-guide tube is by hard material structure At.
CN201711126987.1A 2017-11-15 2017-11-15 A kind of small-sized biological biopsy sample container Active CN108382745B (en)

Priority Applications (1)

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CN201711126987.1A CN108382745B (en) 2017-11-15 2017-11-15 A kind of small-sized biological biopsy sample container

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Application Number Priority Date Filing Date Title
CN201711126987.1A CN108382745B (en) 2017-11-15 2017-11-15 A kind of small-sized biological biopsy sample container

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CN108382745B true CN108382745B (en) 2019-11-15

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Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPP616798A0 (en) * 1998-09-28 1998-10-22 Commonwealth Scientific And Industrial Research Organisation Method and apparatus for the storage of entomapathogenic nematodes
CN200966267Y (en) * 2006-11-09 2007-10-31 王亨利 Tank for storing and transmitting live aquatic product hibernating
CN201604922U (en) * 2010-01-27 2010-10-13 朱勤 Portable type aquatic living body storage and transportation device
KR20120013675A (en) * 2010-08-06 2012-02-15 주식회사 지에스리테일 Container to Store Aquatic Products
CN201770177U (en) * 2010-08-29 2011-03-23 奉化市兴洋水产食品有限公司 Dry/wet two-box shopping barrel
JP2014027914A (en) * 2012-06-28 2014-02-13 Kotomi Suisan Co Ltd Apparatus for transportation and preservation of live bait for fishing and manufacturing method thereof, and transportation and preservation method of live bait for fishing

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