CN108379318A - It is a kind of with the floral disc of sunflower water extract of xanthine oxidase inhibitory activity, preparation method and applications - Google Patents
It is a kind of with the floral disc of sunflower water extract of xanthine oxidase inhibitory activity, preparation method and applications Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 80
- 241000208818 Helianthus Species 0.000 title claims abstract description 56
- 235000003222 Helianthus annuus Nutrition 0.000 title claims abstract description 56
- 239000000284 extract Substances 0.000 title claims abstract description 49
- 108010093894 Xanthine oxidase Proteins 0.000 title claims abstract description 28
- 102100033220 Xanthine oxidase Human genes 0.000 title claims abstract description 28
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000003814 drug Substances 0.000 claims abstract description 19
- 239000000706 filtrate Substances 0.000 claims abstract description 18
- 229940079593 drug Drugs 0.000 claims abstract description 17
- 239000012153 distilled water Substances 0.000 claims abstract description 14
- 230000009514 concussion Effects 0.000 claims abstract description 10
- 239000012043 crude product Substances 0.000 claims abstract description 10
- 238000001556 precipitation Methods 0.000 claims abstract description 8
- 235000013305 food Nutrition 0.000 claims abstract description 7
- 238000004108 freeze drying Methods 0.000 claims abstract description 5
- 210000003734 kidney Anatomy 0.000 claims abstract description 5
- 239000012528 membrane Substances 0.000 claims abstract description 4
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 3
- 210000002700 urine Anatomy 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 3
- 230000001737 promoting effect Effects 0.000 abstract 1
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 14
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 10
- 241000699666 Mus <mouse, genus> Species 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 10
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 9
- 229940109239 creatinine Drugs 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 229940075420 xanthine Drugs 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 4
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 229940116269 uric acid Drugs 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 201000001431 Hyperuricemia Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- OFCNXPDARWKPPY-UHFFFAOYSA-N allopurinol Chemical compound OC1=NC=NC2=C1C=NN2 OFCNXPDARWKPPY-UHFFFAOYSA-N 0.000 description 2
- 229960003459 allopurinol Drugs 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- RYYCJUAHISIHTL-UHFFFAOYSA-N 5-azaorotic acid Chemical compound OC(=O)C1=NC(=O)NC(=O)N1 RYYCJUAHISIHTL-UHFFFAOYSA-N 0.000 description 1
- 208000010444 Acidosis Diseases 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 206010021703 Indifference Diseases 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- BKBGLYZQMLWOAS-UHFFFAOYSA-K [K+].O.[PH2](=O)[O-].[PH2](=O)[O-].[PH2](=O)[O-].[K+].[K+] Chemical compound [K+].O.[PH2](=O)[O-].[PH2](=O)[O-].[PH2](=O)[O-].[K+].[K+] BKBGLYZQMLWOAS-UHFFFAOYSA-K 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 229950000193 oteracil Drugs 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/06—Antigout agents, e.g. antihyperuricemic or uricosuric agents
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Natural Medicines & Medicinal Plants (AREA)
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- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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Abstract
It is a kind of with the floral disc of sunflower water extract of xanthine oxidase inhibitory activity, preparation method and applications, belong to medicine and pharmacology technical field.It is to grind sunflower disk drying, crosses 60 mesh sieve, obtain crude product；3~8g crude products are added in 100mL distilled water, 50~100 DEG C of concussion water carry 1~3h, and 3000~8000rpm/min centrifuges 10~20min, obtains filtrate I；Precipitation is collected, 100mL distilled water is added, concussion water carries, centrifuges and obtain filtrate II under the same terms；Precipitation is collected, 50mL distilled water is added, concussion water carries, centrifuges and obtain filtrate III under the same terms；Merging filtrate I, II and III；Filtrate is subjected to ultrafiltration, then crosses 0.45 μm of filter membrane, sunflower disk water extract of the present invention is obtained after freeze-drying, yield is 25.00~39.20%.Sunflower disk water extract of the present invention has application in terms of preparing anti-trioxypurine and promoting mouse kidney health drug or food.
Description
Technical field
The invention belongs to medicine and pharmacology technical fields, and in particular to a kind of sunflower with xanthine oxidase inhibitory activity
Floral disc water extract, preparation method and applications.
Background technology
Sunflower (helian thus annuus L.) be composite family Helianthus annual plant, originate in North America, now for
One of the oil crops in the world, the whole world are widely planted.It is not only a kind of economic food plant, and is a kind of worth utilization
Medicinal plant, it is sweet in flavor, warm-natured, nontoxic；Major function：Heat-clearing, Li Shui, lead to and urinate, control gastric cancer etc..
Sunflower is widely cultivated in China, and as a kind of important oil crops, sunflower has in the food industry
Highly important application.As its by-product resource floral disc, be often used as animal feeding-stuff, to its medical value research compared with
It is few.
Invention content
The floral disc of sunflower water extract that the purpose of the present invention is to provide a kind of with xanthine oxidase inhibitory activity, system
Preparation Method and its application.
Specifically, sunflower disk water extract of the present invention, yield is 25.00~39.20% (after yield=freeze-drying
Crude product quality × 100% for water extract quality/weigh)
The preparation method of sunflower disk water extract of the present invention, its step are as follows：
(1) sunflower disk drying is ground, crosses 60 mesh sieve, obtains crude product；
(2) 3~8g steps (1) crude product being added in 100mL distilled water, 50~100 DEG C of concussion water carry 1~3h, and 3000
~8000rpm/min centrifuges 10~20min, obtains filtrate I；Precipitation is collected, 100mL distilled water is added, is shaken under the same terms
Water is swung to carry, centrifuge and obtain filtrate II；Precipitation is collected, 50mL distilled water is added, concussion water carries, centrifuges and filtered under the same terms
Liquid III；Merging filtrate I, II and III；
(3) filtrate for obtaining step (2) carries out ultrafiltration, then crosses 0.45 μm of filter membrane, the present invention is obtained after freeze-drying
The sunflower disk water extract.
The present invention also provides application of the above-mentioned sunflower disk water extract in terms of preparing anti-trioxypurine drug or food.
Invention further provides above-mentioned sunflower disk water extracts to prepare promotion mouse kidney health drug or food side
The application in face.
Description of the drawings
Fig. 1：For the external XO inhibitory activity curve of sunflower disk water extract under different temperatures of the present invention；
Fig. 1 shows that sunflower disk water extract has good inhibition to xanthine oxidase (XO).At identical temperature, XO
Inhibiting rate has certain concentration-dependent relation.Under 20mg/mL concentration conditions, the inhibiting rate highest of 100 DEG C of sunflower disk water extracts,
Reach 84.94 ± 0.69%.
Fig. 2：Influence result schematic diagram for sunflower disk water extract of the present invention to mouse serum uric acid level.Wherein*P<
0.05, it indicates compared with normal blank group, difference reaches the level of signifiance；**P<0.01, it indicates compared with normal blank group, difference
Reach the pole level of signifiance；#P<0.05, it indicates compared with model group, difference reaches the level of signifiance；##P<0.01, indicate and model group
Compare, difference reaches the pole level of signifiance；&P<0.05, it indicates compared with positive drug group, difference reaches the level of signifiance；&&P<
0.01, it indicates compared with positive drug group, difference reaches the pole level of signifiance.
The sunflower disk water extract of the present invention has certain anti-trioxypurine effect to Studies on Animal Models of Hyperuricemic Mice；Sunflower disk water
The serum uric acid level of the basic, normal, high dosage of extract, compared with model group, hence it is evident that reduce, difference reaches the level of signifiance, respectively
102.62,132.16,158.49 μm of ol/L, close to normal blank group.Show that sunflower disk water extract can reduce blood urine sour water
It is flat.
Fig. 3：It is sunflower disk water extract of the present invention on the active influence result schematic diagrams of XO in mouse (a) serum (b) liver.
Wherein*P<0.05, it indicates compared with normal blank group, difference reaches the level of signifiance；**P<0.01, it indicates and normal blank group ratio
Compared with difference reaches the pole level of signifiance；#P<0.05, it indicates compared with model group, difference reaches the level of signifiance；##P<0.01, it indicates
Compared with model group, difference reaches the pole level of signifiance；&P<0.05, it indicates compared with positive drug group, difference reaches notable water
It is flat；&&P<0.01, it indicates compared with positive drug group, difference reaches the pole level of signifiance.
The sunflower disk water extract of the present invention presses down the anti-trioxypurine mechanism of Studies on Animal Models of Hyperuricemic Mice with xanthine oxidase
System may be related；Scheme (a), is influence of the sunflower disk water extract to XO vigor in serum.As can be seen that compared with model group, certain herbaceous plants with big flowers
XO vigor decreases compared with model group in the low middle high dose group serum of floral disc water extract, reduces 21.91%, 20.60% respectively,
5.33%.Wherein middle low dose group, difference reach the level of signifiance.It is shadow of the sunflower disk water extract to XO vigor in liver to scheme (b)
It rings.As can be seen that compared with model group, XO vigor decreases in the low middle high dose group serum of sunflower disk water extract, drops respectively
Low 7.7%, 11.64%, 11.46%.Wherein middle high dose group, difference reach the level of signifiance.
Fig. 4：Influence result schematic diagram for sunflower disk water extract of the present invention to mouse (a) urea nitrogen (b) creatinine.Wherein*P
<0.05, it indicates compared with normal blank group, difference reaches the level of signifiance；**P<0.01, it indicates compared with normal blank group, difference
Reach the pole level of signifiance；#P<0.05, it indicates compared with model group, difference reaches the level of signifiance；##P<0.01, indicate and model group
Compare, difference reaches the pole level of signifiance；&P<0.05, it indicates compared with positive drug group, difference reaches the level of signifiance；&&P<
0.01, it indicates compared with positive drug group, difference reaches the pole level of signifiance.
The sunflower disk water extract of the present invention measures the urea nitrogen and creatinine level of Studies on Animal Models of Hyperuricemic Mice, illustrates this
There is no toxic side effect to mouse health under experiment condition.Scheme the influence that (a) is sunflower disk water extract mouse urea nitrogen, can see
Go out, normal blank group urea nitrogen is 10.31mmol/L, and model group 11.34mmol/L, group difference is not notable, shows high urine
Acidaemia model group will not cause to damage to kidney of mouse；Positive drug group urea nitrogen reaches 36.04mmol/L, shows not
Fast alcohol seriously destroys nephridial tissue；And the low middle high dose group of sunflower disk water extract reduces urea nitrogen to 9.73,10.62,
10.48mmol/L is not notable with normal blank group difference.Showing sunflower disk water extract hardly influences nephridial tissue health.Figure
(b) be sunflower disk water extract mice serum creatinine influence, it can be seen that positive drug group so that creatinine level is sharply increased for
63.16μmol/L；And the low middle high dose group creatinine in serum level of sunflower disk water extract substantially with normal blank group indifference.Table
It is bright that low middle high dose group hardly causes injury of kidney compared with positive drug is to the damage of nephridial tissue, as a result with urea nitrogen knot
Fruit is consistent.
Specific implementation mode
Embodiment 1：The preparation of sunflower disk water extract and its external XO inhibitory activity
1. the preparation of sunflower disk water extract
(1) sample pre-treatments：Sunflower disk pulper is ground, 60 mesh sieve is crossed, it is spare to obtain crude product collection.
(2) 5g steps (1) crude product is weighed respectively, is respectively put into conical flask, 100 mL distilled water are added in each conical flask,
(50,60,70,80,90,100 DEG C) concussion water carry 2h at different temperatures respectively, and 5000rpm/min centrifuges 10min, filtered
Liquid I；Precipitation is collected, 100mL distilled water is added, concussion water carries 1h at identical temperature, and 5000rpm/min centrifuges 10min, obtains
Filtrate II；Precipitation to be collected, 50 mL distilled water are added, concussion water carries 1h at identical temperature, and 5000rpm/min centrifuges 10min,
Obtain filtrate III；Merging filtrate.Filtrate will be obtained, ultrafiltration is carried out, 0.45 μm of filter membrane is crossed, is freeze-dried, will be collected.Respectively
To the sunflower disk water extract prepared at different temperatures.Yield=water upgrading amount after freeze-drying/weighs crude product quality × 100%, knot
Fruit is as shown in table 1.
Table 1：Sunflower disk water extract yield
2.HPLC methods measure the reaction condition of external XO inhibitory activity
Prepare before experiment：
Phosphate buffer phosphate buffer is prepared：Weigh 0.478g potassium dihydrogen phosphates, tri- hypophosphite monohydrate potassium dihydrogens of 3.473g,
9.31mg EDTA-2Na are added in conical flask in 250mL distilled water, adjust pH to 7.4.
Preparation of samples：Sunflower disk water extract is dissolved in buffer solution phosphate buffer, is configured to the mother liquor of 100mg/mL, then
Mother liquor is diluted to the working solution of 12.5,25,50,75mg/mL, it is spare.
This experiment is based on xanthine and generates uric acid under xanthine oxidase catalysis, and by fast to Huang at 254nm wavelength
The characteristic absorption of purine carrys out the variation of detection substrate xanthine amount, to calculate inhibiting rate of the sunflower disk water extract to XO.But in order to
It is Interference Peaks to be not present at xanthine, i.e., in Suitable assays condition to ensure the accuracy of experiment, satisfactory experiment condition
Under, the independent peak of xanthine can be obtained.Therefore preliminary experiment is carried out to the amount of enzyme and substrate, finally obtains enzyme reaction system：200
400 μ L of sunflower disk water extract addition, the 0.02U/mL XO of μ L various concentrations, different preparation temperatures, 37 DEG C of incubation 15min；Again plus
Enter 400 μ L, 1.0mmol/L xanthine starts reaction, 37 DEG C of reaction 40min, 500 μ L of addition, 1M HCl termination reactions.HPLC colors
Spectral condition：Chromatographic column：C18 pillars, UV detector：254nm, column temperature：25 DEG C, mobile phase：90% (v/v), 0.02mmol/L
Potassium dihydrogen phosphate+10% (v/v) methanol, flow velocity：1mL/min, applied sample amount：20μL.Obtain that the results are shown in Table 1：
Table 2：The external XO inhibiting effect data of sunflower disk water extract
Experimental result
It may indicate that the sunflower disk water extract of the present invention has external XO inhibitory activity well from table 2 and Fig. 1, to subtract
Oliguresis acid generates.
Embodiment 2：Anti-trioxypurine and safety effect in sunflower disk water extract body
One, experiment material
Animal：Kunming mouse, male, weight (20 ± 2g) are provided by Jilin University's Life Science College experimental technique platform；
Sample is administered：Normal blank group (distilled water), hyperuricemia model group, positive drug group (Allopurinol), of the present invention group of (sunflower
Disk water extract, basic, normal, high, dosage group).
Two, experimental method
Male mice 60 is taken, adapts to after a week, weed out unqualified mouse, be randomly divided into normal blank group, high lithemia
Blood stasis model group, positive drug group and this experiment invention group (low, middle and high dose groups), every group 9.In addition to normal group, often
Group gavage hypoxanthine (500mg/kg), while Oteracil Potassium (100 mg/kg) is injected intraperitoneally, carry out modeling.It is normal empty after 1h
The physiological saline of white group and hyperuricemia model group gavage 0.2mL, positive drug group gastric infusion Allopurinol (10mg/kg),
Of the present invention group of low middle high dose group difference gastric infusion (0.5g/kg, 1.0g/kg, 1.5g/kg), continuous 8 days.Eyeball of mouse takes
Blood detaches serum, measures its uric acid concentration, xanthine oxidase activity, urea nitrogen and creatinine value.Obtain result such as Fig. 2, figure
3, Fig. 4.
Three, experimental result
Above-mentioned Fig. 2, Fig. 3, Fig. 4 the experimental results showed that, sunflower disk water extract of the invention can reduce blood urine in Mice Body
Sour water is flat, reduces internal XO activity, while can be substantially reduced mouse urea nitrogen and creatinine, improves renal function.
Claims (4)
1. a kind of preparation method of the floral disc of sunflower water extract with xanthine oxidase inhibitory activity, its step are as follows：
(1) sunflower disk drying is ground, crosses 60 mesh sieve, obtains crude product；
(2) 3~8g steps (1) crude product being added in 100mL distilled water, 50~100 DEG C of concussion water carry 1~3h, 3000~
8000rpm/min centrifuges 10~20min, obtains filtrate I；Precipitation is collected, 100mL distilled water is added, is shaken under the same terms
Water carries, centrifuges and obtains filtrate II；Precipitation is collected, 50mL distilled water is added, concussion water carries, centrifuges and obtain filtrate under the same terms
Ⅲ；Merging filtrate I, II and III；
(3) filtrate for obtaining step (2) carries out ultrafiltration, then crosses 0.45 μm of filter membrane, sunflower disk water is obtained after freeze-drying and is carried
Object.
2. a kind of floral disc of sunflower water extract with xanthine oxidase inhibitory activity, it is characterised in that：It is by claim 1
The method is prepared.
3. a kind of floral disc of sunflower water extract with xanthine oxidase inhibitory activity described in claim 2 is preparing drop urine
Application in terms of sour drug or food.
4. a kind of floral disc of sunflower water extract with xanthine oxidase inhibitory activity described in claim 2 is preparing promotion
Application in terms of mouse kidney health drug or food.
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| CN201810544670.8A CN108379318A (en) | 2018-05-31 | 2018-05-31 | It is a kind of with the floral disc of sunflower water extract of xanthine oxidase inhibitory activity, preparation method and applications |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810544670.8A CN108379318A (en) | 2018-05-31 | 2018-05-31 | It is a kind of with the floral disc of sunflower water extract of xanthine oxidase inhibitory activity, preparation method and applications |
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| CN108379318A true CN108379318A (en) | 2018-08-10 |
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| CN201810544670.8A Pending CN108379318A (en) | 2018-05-31 | 2018-05-31 | It is a kind of with the floral disc of sunflower water extract of xanthine oxidase inhibitory activity, preparation method and applications |
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| CN (1) | CN108379318A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116808172A (en) * | 2023-01-07 | 2023-09-29 | 王晓娟 | Sunflower disc liposome and application thereof in preparation of products for reducing uric acid and dissolving tophus |
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|---|---|---|---|---|
| CN105920067A (en) * | 2016-05-06 | 2016-09-07 | 洮南市金塔生物科技有限公司 | Sunflower calathide extract containing polysaccharide, flavonoid and alkaloid and preparation method of sunflower calathide extract |
| CN106420876A (en) * | 2016-11-16 | 2017-02-22 | 马业忠 | Drinking liquid with uric acid decreasing and lipid lowering effects and preparation method of drinking liquid |
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2018
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105920067A (en) * | 2016-05-06 | 2016-09-07 | 洮南市金塔生物科技有限公司 | Sunflower calathide extract containing polysaccharide, flavonoid and alkaloid and preparation method of sunflower calathide extract |
| CN106420876A (en) * | 2016-11-16 | 2017-02-22 | 马业忠 | Drinking liquid with uric acid decreasing and lipid lowering effects and preparation method of drinking liquid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116808172A (en) * | 2023-01-07 | 2023-09-29 | 王晓娟 | Sunflower disc liposome and application thereof in preparation of products for reducing uric acid and dissolving tophus |
| CN116808172B (en) * | 2023-01-07 | 2024-02-13 | 龚曙初 | Sunflower disc peptide, composite liposome and application thereof in preparation of products for reducing uric acid and dissolving tophus |
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