CN108378061A - 一种油用牡丹叶斑病诱抗剂 - Google Patents

一种油用牡丹叶斑病诱抗剂 Download PDF

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CN108378061A
CN108378061A CN201810434663.2A CN201810434663A CN108378061A CN 108378061 A CN108378061 A CN 108378061A CN 201810434663 A CN201810434663 A CN 201810434663A CN 108378061 A CN108378061 A CN 108378061A
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CN108378061B (zh
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刘高峰
张利
周天华
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Heze University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26—Phosphorus; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A01N37/04—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof polybasic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof

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Abstract

本发明公开了一种油用牡丹叶斑病诱抗剂，该诱抗剂为由草酸、FeSO4、脱落酸(ABA)、壳聚糖、KH2PO4和适量水配置成的悬浮剂；所述悬浮剂中含有：草酸30mMol/L，FeSO410mMol/L，脱落酸(ABA)20mMol/L，壳聚糖300mg/L，KH2PO40.5％。通过本发明的诱抗剂可以诱导油用牡丹产生抗病性，可在很大程度上降低农药的使用量，减少农药残留，还可促进油用牡丹生长，提高其全株系统抗性，为农民节省生产成本同时也可促进产量的提高，增加经济效益。

Description

一种油用牡丹叶斑病诱抗剂
技术领域
本发明涉及生物病害防控领域，具体涉及一种油用牡丹叶斑病诱抗剂。
背景技术
当前我国农药市场上普遍存在老产品多、同质化严重，产品效果良莠不齐、应用效果差、残留多的现象。
发明内容
本申请人根据多年植物抗性诱导方面的研究，结合在油用牡丹上的实验结果，研发出了一种油用牡丹叶部病害诱抗剂，可对油用牡丹叶部病害起到明显的预防效果。
为实现上述目的，本发明采取的技术方案为：
一种油用牡丹叶斑病诱抗剂，该诱抗剂为由草酸、FeSO4、脱落酸(ABA)、壳聚糖、KH2PO4和适量水配置成的悬浮剂；所述悬浮剂中含有：
草酸30mMol/L，FeSO410mMol/L，脱落酸(ABA)20mMol/L，壳聚糖300mg/L，KH2PO40.5％。
本发明的一种油用牡丹叶斑病诱抗剂在花后15天喷施到油用牡丹叶面上，7天后再喷一次。
本发明具有以下有益效果：
1、草酸与FeSO4、ABA、KH2PO4均可在一定程度上诱导油用牡丹叶片抗性酶过氧化物酶、超氧化物歧化酶、过氧化氢酶活性的升高，提高油用牡丹叶片对叶斑病的抗性；组合后的防病效果明显。
2、壳聚糖可在牡丹叶片表面形成一层均匀的保护膜，可明显抑制叶斑病病原菌孢子的产生、萌发以及菌丝生长。
3、草酸作为壳聚糖的有机溶剂，起到高效质子供体作用，可增加壳聚糖的溶解性，还可提高壳聚糖的抑菌性。
4、KH2PO4可作为一种营养补充，在起到防病的同时，满足花后植物营养需求，一举两得。
附图说明
图1为本发明实施例2中不同处理对油用牡丹抗叶斑病的病情指数的影响示意图。
图2为本发明实施例3中不同处理对油用牡丹抗叶斑病的病情指数的影响示意图。
具体实施方式
为了使本发明的目的及优点更加清楚明白，以下结合实施例对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。
实施例1
按草酸30mMol/L，FeSO410mMol/L，脱落酸(ABA)20mMol/L，壳聚糖300mg/L，KH2PO40.5％的比例称取各组分配置成悬浮液，即得诱抗剂。
实施例2
通过叶斑病悬浮孢子接种实验，以灭菌蒸馏水做对照，分别进行了水处理、草酸单独处理、NO单独处理、实施例1所得的诱抗剂处理，接种后第15天对比其病情指数。结果如图1所示，实施例所得的诱抗剂效果明显好于其他处理，病情指数明显低于对照，是对照的51.33％。
实施例3
以蒸馏水做对照，进行了草酸、ABA、实施例1所得的诱抗剂处理。许多研究表明，POD酶是植物叶片抗性酶之一，与植物的抗病性密切相关。结果表明，各处理中实施例所得的诱抗剂效果表现最好，可明显提高油用牡丹叶片POD酶活性，与对照相比提高了23.36％。
以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以作出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。

Claims (3)

1.一种油用牡丹叶斑病诱抗剂，其特征在于：该诱抗剂为由草酸、FeSO4、脱落酸、壳聚糖、KH2PO4和适量水配置成的悬浮剂。
2.如权利要求1所述的一种油用牡丹叶斑病诱抗剂，其特征在于：所述悬浮剂中含有：
草酸30mMol/L，FeSO410mMol/L，脱落酸20mMol/L，壳聚糖300mg/L，KH2PO40.5％。
3.如权利要求1所述的一种油用牡丹叶斑病诱抗剂的使用方式，其特征在于，在花后15天喷施到油用牡丹叶面上，7天后再喷一次。
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CN112913860A (zh) * 2021-02-01 2021-06-08 河北科技师范学院 一种防治农作物病菌的杀菌剂的制备方法

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CN109797109A (zh) * 2019-03-22 2019-05-24 河南科技大学 一种牡丹腔孢叶斑病菌分离筛选培养基及其应用
CN112913860A (zh) * 2021-02-01 2021-06-08 河北科技师范学院 一种防治农作物病菌的杀菌剂的制备方法

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