CN108344618A - A kind of malachite green fast detecting method - Google Patents
A kind of malachite green fast detecting method Download PDFInfo
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- CN108344618A CN108344618A CN201810020745.2A CN201810020745A CN108344618A CN 108344618 A CN108344618 A CN 108344618A CN 201810020745 A CN201810020745 A CN 201810020745A CN 108344618 A CN108344618 A CN 108344618A
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Abstract
The invention discloses a kind of malachite green fast detecting methods, using sodium sulfite or sodium thiosulfate as sensitizer, malachite green solution is added in the buffer solution containing the sensitizer, electrode is statically placed in the sensitizer buffer solution containing malachite green and carries out open circuit enrichment, malachite green occurs addition reaction with sodium sulfite and forms intermediate product, the intermediate product is enriched on electrode, it is detected with Square wave anodic stripping voltammetry method, obtain the standard curve that Stripping Currents change with malachite green solution concentration, recycle Square wave anodic stripping voltammetry method and standard curve, judge the concentration of malachite green solution to be measured, that is the content of malachite green.The preparation of the detection method reagent and detection process do not need expensive instrument and reagent, of low cost, easy to operate, and detection sensitivity is high, and test result is steady, have good market application prospect.
Description
Technical field
The present invention relates to organic compounds to test and analyze field, specially a kind of malachite green fast detecting method.
Background technology
Malachite green is a kind of triphenylmenthane type green dye, is commonly called as Viride Nitens, aniline green etc., is widely used in leather row
Industry, paper-making industry and textile printing and dyeing industry.Malachite green is also a kind of sterilization and insecticide that performance is extremely excellent simultaneously, for
The common disease of a variety of aquatic products such as fish, shrimp such as saprolegniasis, branchiomycosis and ich etc. have good killing effect；But malachite
The green rapid metabolization in fish body is leucomalachite green, and leucomalachite green has higher toxicity, and easily residual, has simultaneously
Teratogenesis, carcinogenesis disable medicine for the aquaculture that majority state is prohibited.But since malachite green is cheap, effect
Fruit is excellent and there are currently no corresponding substitute products, thus remains incessant after repeated prohibition.Malachite green common detection methods are concentrated mainly on two
Class, one kind be Physico-chemical tests method, it is another kind of be enzyme-linked immune detection method, these methods or be qualitative detection, Huo Zhexu
Want large-scale instrument and professional operator, or there are false positive issue and expensive, since it is desired that invention it is a kind of it is simple,
Effectively, specificity height and low-cost detection method.
Invention content
To solve the above-mentioned problems, the present invention provides a kind of malachite green fast detecting methods, with sodium sulfite or sulphur
Sodium thiosulfate is sensitizer, and malachite green solution is added in the buffer solution containing the sensitizer, electrode is statically placed in
Open circuit enrichment is carried out in sensitizer buffer solution containing malachite green, malachite green occurs addition reaction with sodium sulfite and formed
Intermediate product, the intermediate product are enriched on electrode, are detected with Square wave anodic stripping voltammetry method, obtain Stripping Currents with
The standard curve of malachite green solution concentration variation, recycles Square wave anodic stripping voltammetry method and standard curve, judges to be measured
The concentration of malachite green solution, the i.e. content of malachite green.The preparation of the detection method reagent and detection process need not be held high
Expensive instrument and reagent, of low cost, easy to operate, detection sensitivity is high, and test result is steady, before having the application of good market
Scape.
The technical scheme is that：
A kind of malachite green fast detecting method, which is characterized in that specifically include following steps：
1) sodium sulfite solution or hypo solution are added to acetate buffer solution or phosphate-buffered is molten
In liquid, it is uniformly mixed, obtains sensitizer mixed liquor；
2) electrode being connected on electrochemical workstation is immersed in step 1) the sensitizer mixed liquor, chooses electrochemistry work
Anodic stripping voltammetry on standing carries out electrode activation to the electrode；
3) after the completion of the electrode activation, it is mixed that malachite green solution is added to the sensitizer in step 2) immersed with electrode
It closes in liquid, stands 5-10 minutes；
4) after the completion of standing, the anodic stripping voltammetry chosen on electrochemical workstation is detected, and is obtained in step 3)
The Stripping Voltammetry figure of the malachite green solution Malachite Green；
5) step 1) is repeated to step 4), determines the Stripping Voltammetry of various concentration malachite green solution Malachite Green
Figure, and the standard curve that Stripping Currents change with malachite green solution concentration is drawn out according to the voltammogram；
6) step 1) is repeated to the Stripping Voltammetry figure for 4) measuring malachite green solution Malachite Green to be measured, according to the volt
Antu obtains Stripping Currents, using standard curve described in step 5), obtains the concentration of malachite green solution to be measured.
The acetate buffer solution is the acetum of acetate, pH value 4.0-5.0.
In the step 1), the molar ratio of sodium sulfite or sodium thiosulfate and acetate in acetate buffer solution is
0.002:1。
The sodium sulfite solution of 2 a concentration of 0.1mol/L of μ L or hypo solution are added to 1mL by the step 1)
In the acetate buffer solution of a concentration of 0.1mol/L, it is uniformly mixed, obtains the sensitizer mixed liquor.
The electrode is screen printing electrode.
The screen printing electrode is three electrode screen printing electrode plates.
The anodic stripping voltammetry is Square wave anodic stripping voltammetry method.
The step 5) and step 6) Malachite Green solution concentration be followed successively by 36ppb, 72ppb, 108ppb, 144ppb,
180ppb、225ppb、270ppb。
During the electrode activation, current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency 15Hz are detected.
When anodic stripping voltammetry in the step 4) on selection electrochemical workstation is detected, current potential is detected
0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency 15Hz.
The present invention technique effect be：
Malachite green fast detecting method of the present invention, sodium sulfite or sodium thiosulfate are with malachite green in acetate
Addition reaction occurs under buffer solution environment and forms intermediate, specific reaction is as follows：
The reaction intermediate is：
The reaction intermediate can reduce the detection current potential of malachite green, and it is mixed that electrode is placed in the enhanced sensitivity dissolved with malachite green
It closes in liquid, the reaction intermediate of generation is easy to be enriched to electrode surface, to achieve the effect that signal amplification, especially intermediate
Electrode enrichment process be open circuit enrichment, i.e., in the case where disconnecting power supply carry out electrode stand enrichment, this process is one
Determine to improve the sensitivity of detection in degree.Electrode is enriched to certain reaction intermediate after standing, recycles portable
Electrochemical workstation carries out anodic stripping voltammetry detection, can live, simple, quick, highly sensitive detection to build one kind
The detection platform of malachite green.
In addition, in the detection process, using screen printing electrode, electrode is at low cost, instant throw, is easy to operate, surveying
It is also highly stable to try performance；It is detected using Square wave anodic stripping voltammetry method, volt-ampere curve is clear, and variation tendency is apparent.
Detection method malachite green detection limit of the present invention can be down to 100nM, and detection time is short, without previous detection
The expensive reagents such as antibody required when sensor are built in method, it is only necessary to which micro sulphite or thiosulfate drops significantly
Low testing cost；The method is easy to operate, detection sensitivity is high；It is not needed during the structure of sensor and use simultaneously
Large-scale instrument and professional operator are relied on, the scene in the rural area and developing country that can be widely used for scarcity of resources is fixed
Amount detection.
Description of the drawings
Fig. 1 is the Stripping Voltammetry of a concentration of 36ppb-270ppb malachite green solutions Malachite Green in the embodiment of the present invention
Figure.
Fig. 2 is the linear fit of 36ppb-270ppb malachite green solutions concentration and Stripping Currents in the embodiment of the present invention
Figure.
Specific implementation mode
Technical scheme of the present invention is described in detail below by specific embodiment, but the scope of the present invention
It is not restricted by the embodiments.
Electrochemical workstation used is portable electrochemical work station in the embodiment of the present invention.
Ppm=mg/L of the present invention, ppb=μ g/L.
Embodiment 1
Present embodiments provide a kind of malachite green fast detecting method, including following step：
1) a concentration of 0.1mol/L and pH=4.5 sodium acetates of 1mL are added in 2 μ L0.1mol/L hypo solutions
Buffer solution, shaken well obtain sensitizer mixed liquor；
2) after screen printing electrode being connected with portable electrochemical work station, the sensitizer immersed obtained by step 1) is mixed
It closes in liquid, chooses Square wave anodic stripping voltammetry method, activation potential window 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency
Rate 15Hz carries out the activation of screen printing electrode；
3) it after the completion of activating, is added in step 2) is immersed with the sensitizer mixed liquor of screen printing electrode a concentration of
36ppb malachite green solutions carry out the pretreatment of malachite green, stand 5 minutes；
4) after the completion of standing, malachite green forms intermediary with sodium sulfite, using Square wave anodic stripping voltammetry method to molten
Liquid system is detected, parameter setting：Current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V is detected, frequency 15Hz is obtained
To volt-ampere curve.
Embodiment 2
Present embodiments provide a kind of malachite green fast detecting method, including following step：
1) a concentration of 0.1mol/L and pH=4.5 sodium acetates that 1mL is added in 2 μ L0.1mol/L sodium sulfite solutions are slow
Solution is rushed, shaken well obtains sensitizer mixed liquor；
2) after screen printing electrode being connected with portable electrochemical work station, the sensitizer immersed obtained by step 1) is mixed
It closes in liquid, chooses Square wave anodic stripping voltammetry method, activation potential window 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency
Rate 15Hz carries out the activation of screen printing electrode；
3) it after the completion of activating, is added in step 2) is immersed with the sensitizer mixed liquor of screen printing electrode a concentration of
36ppb malachite green solutions carry out the pretreatment of malachite green, stand 5 minutes；
4) after the completion of standing, malachite green forms intermediary with sodium sulfite, using Square wave anodic stripping voltammetry method to molten
Liquid system is detected, parameter setting：Current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V is detected, frequency 15Hz is obtained
To volt-ampere curve, as shown in a curves in Fig. 1.
By analyzing Examples 1 and 2, based on selection example 2, various concentration malachite green solution is measured.
Embodiment 3
Present embodiments provide a kind of malachite green fast detecting method, including following step：
1) a concentration of 0.1mol/L and pH=4.5 sodium acetates that 1mL is added in 2 μ L0.1mol/L sodium sulfite solutions are slow
Solution is rushed, shaken well obtains sensitizer mixed liquor；
2) after screen printing electrode being connected with portable electrochemical work station, the sensitizer immersed obtained by step 1) is mixed
It closes in liquid, chooses Square wave anodic stripping voltammetry method, activation potential window 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency
Rate 15Hz carries out the activation of screen printing electrode；
3) it after the completion of activating, is added in step 2) is immersed with the sensitizer mixed liquor of screen printing electrode a concentration of
72ppb malachite green solutions carry out the pretreatment of malachite green, stand 5 minutes；
4) after the completion of standing, malachite green forms intermediary with sodium sulfite, using Square wave anodic stripping voltammetry method to molten
Liquid system is detected, parameter setting：Current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V is detected, frequency 15Hz is obtained
To volt-ampere curve, as shown in b curves in Fig. 1.
Embodiment 4
Present embodiments provide a kind of malachite green fast detecting method, including following step：
1) a concentration of 0.1mol/L and pH=4.5 sodium acetates that 1mL is added in 2 μ L0.1mol/L sodium sulfite solutions are slow
Solution is rushed, shaken well obtains sensitizer mixed liquor；
2) after screen printing electrode being connected with portable electrochemical work station, the sensitizer immersed obtained by step 1) is mixed
It closes in liquid, chooses Square wave anodic stripping voltammetry method, activation potential window 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency
Rate 15Hz carries out the activation of screen printing electrode；
3) it after the completion of activating, is added in step 2) is immersed with the sensitizer mixed liquor of screen printing electrode a concentration of
108ppb malachite green solutions carry out the pretreatment of malachite green, stand 5 minutes；
4) after the completion of standing, malachite green forms intermediary with sodium sulfite, using Square wave anodic stripping voltammetry method to molten
Liquid system is detected, parameter setting：Current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V is detected, frequency 15Hz is obtained
To volt-ampere curve, as shown in c curves in Fig. 1.
Embodiment 5
Present embodiments provide a kind of malachite green fast detecting method, including following step：
1) a concentration of 0.1mol/L and pH=4.5 sodium acetates that 1mL is added in 2 μ L0.1mol/L sodium sulfite solutions are slow
Solution is rushed, shaken well obtains sensitizer mixed liquor；
2) after screen printing electrode being connected with portable electrochemical work station, the sensitizer immersed obtained by step 1) is mixed
It closes in liquid, chooses Square wave anodic stripping voltammetry method, activation potential window 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency
Rate 15Hz carries out the activation of screen printing electrode；
3) it after the completion of activating, is added in step 2) is immersed with the sensitizer mixed liquor of screen printing electrode a concentration of
144ppb malachite green solutions carry out the pretreatment of malachite green, stand 5 minutes；
4) after the completion of standing, malachite green forms intermediary with sodium sulfite, using Square wave anodic stripping voltammetry method to molten
Liquid system is detected, parameter setting：Current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V is detected, frequency 15Hz is obtained
To volt-ampere curve, as shown in d curves in Fig. 1.
Embodiment 6
Present embodiments provide a kind of malachite green fast detecting method, including following step：
1) a concentration of 0.1mol/L and pH=4.5 sodium acetates that 1mL is added in 2 μ L0.1mol/L sodium sulfite solutions are slow
Solution is rushed, shaken well obtains sensitizer mixed liquor；
2) after screen printing electrode being connected with portable electrochemical work station, the sensitizer immersed obtained by step 1) is mixed
It closes in liquid, chooses Square wave anodic stripping voltammetry method, activation potential window 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency
Rate 15Hz carries out the activation of screen printing electrode；
3) it after the completion of activating, is added in step 2) is immersed with the sensitizer mixed liquor of screen printing electrode a concentration of
180ppb malachite green solutions carry out the pretreatment of malachite green, stand 5 minutes；
4) after the completion of standing, malachite green forms intermediary with sodium sulfite, using Square wave anodic stripping voltammetry method to molten
Liquid system is detected, parameter setting：Current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V is detected, frequency 15Hz is obtained
To volt-ampere curve, as shown in e curves in Fig. 1.
Embodiment 7
Present embodiments provide a kind of malachite green fast detecting method, including following step：
1) a concentration of 0.1mol/L and pH=4.5 sodium acetates that 1mL is added in 2 μ L0.1mol/L sodium sulfite solutions are slow
Solution is rushed, shaken well obtains sensitizer mixed liquor；
2) after screen printing electrode being connected with portable electrochemical work station, the sensitizer immersed obtained by step 1) is mixed
It closes in liquid, chooses Square wave anodic stripping voltammetry method, activation potential window 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency
Rate 15Hz carries out the activation of screen printing electrode；
3) it after the completion of activating, is added in step 2) is immersed with the sensitizer mixed liquor of screen printing electrode a concentration of
225ppb malachite green solutions carry out the pretreatment of malachite green, stand 5 minutes；
4) after the completion of standing, malachite green forms intermediary with sodium sulfite, using Square wave anodic stripping voltammetry method to molten
Liquid system is detected, parameter setting：Current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V is detected, frequency 15Hz is obtained
To volt-ampere curve, as shown in f curves in Fig. 1.
Embodiment 8
Present embodiments provide a kind of malachite green fast detecting method, including following step：
1) a concentration of 0.1mol/L and pH=4.5 sodium acetates that 1mL is added in 2 μ L0.1mol/L sodium sulfite solutions are slow
Solution is rushed, shaken well obtains sensitizer mixed liquor；
2) after screen printing electrode being connected with portable electrochemical work station, the sensitizer immersed obtained by step 1) is mixed
It closes in liquid, chooses Square wave anodic stripping voltammetry method, activation potential window 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency
Rate 15Hz carries out the activation of screen printing electrode；
3) it after the completion of activating, is added in step 2) is immersed with the sensitizer mixed liquor of screen printing electrode a concentration of
270ppb malachite green solutions carry out the pretreatment of malachite green, stand 5 minutes；
4) after the completion of standing, malachite green forms intermediary with sodium sulfite, using Square wave anodic stripping voltammetry method to molten
Liquid system is detected, parameter setting：Current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V is detected, frequency 15Hz is obtained
To volt-ampere curve, as shown in g curves in Fig. 1.
According to embodiment 2-8, the concentration of measurement be respectively 36ppb, 72ppb, 108ppb, 144ppb, 180ppb,
The Stripping Voltammetry figure of the malachite green solution Malachite Green of 225ppb, 270ppb, and dissolution is drawn out according to the voltammogram
The standard curve that electric current changes with malachite green solution concentration, the i.e. linear fit of malachite green solution concentration and Stripping Currents
Figure, as shown in Figure 2.
Embodiment 9
1) a concentration of 0.1mol/L and pH=4.5 sodium acetates that 1mL is added in 2 μ L0.1mol/L sodium sulfite solutions are slow
Solution is rushed, shaken well obtains sensitizer mixed liquor；
2) after screen printing electrode being connected with portable electrochemical work station, the sensitizer immersed obtained by step 1) is mixed
It closes in liquid, chooses Square wave anodic stripping voltammetry method, activation potential window 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency
Rate 15Hz carries out the activation of screen printing electrode；
3) after the completion of activating, peacock to be measured is added in step 2) is immersed with the sensitizer mixed liquor of screen printing electrode
Malachite green solution carries out the pretreatment of malachite green, stands 5 minutes；
4) after the completion of standing, malachite green forms intermediary with sodium sulfite, using Square wave anodic stripping voltammetry method to molten
Liquid system is detected, parameter setting：Current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V is detected, frequency 15Hz is obtained
To volt-ampere curve, it is 0.2 μ A to obtain the Stripping Currents under this concentration according to the volt-ampere curve, according to 0.2 μ of the Stripping Currents
A, corresponding standard curve shown in Fig. 2 obtain a concentration of 201ppb of malachite green to be measured.
Detection method of the present invention is not necessarily to build the expensive reagents such as antibody required when sensor in previous detection method,
Micro sulphite or thiosulfate are only needed, testing cost is greatly reduced；And detection time is short, easy to operate, detection
High sensitivity；The use of screen printing electrode, electrode is at low cost, it is instant throw, easy to operate, test performance is also highly stable；
It is detected using Square wave anodic stripping voltammetry method, curvilinear characteristic of bending over the desk is apparent；Simultaneously the detection method sensor structure and make
With large-scale instrument and professional operator is needed not rely in the process, rural area and the development of scarcity of resources can be widely used for
The in situ quantitation of middle country detects.
Not the restriction to invention it should be pointed out that above example is explanation of the invention, without prejudice to
In the case of the spirit of the present invention, the present invention can make any type of modification, such as sulfite concentration, sodium hydroxide are dense
Degree, activation potential, soak time, pretreatment current potential, pretreatment time and detection parameters variation all should be in technical solution of the present invention
Within protection domain.
Claims (10)
1. a kind of malachite green fast detecting method, which is characterized in that specifically include following steps：
1) sodium sulfite solution or hypo solution are added in acetate buffer solution or phosphate buffer solution,
It is uniformly mixed, obtains sensitizer mixed liquor；
2) electrode being connected on electrochemical workstation is immersed in step 1) the sensitizer mixed liquor, chooses electrochemical workstation
On anodic stripping voltammetry to the electrode carry out electrode activation；
3) after the completion of the electrode activation, malachite green solution is added to the sensitizer mixed liquor in step 2) immersed with electrode
In, stand 5-10 minutes；
4) after the completion of standing, the anodic stripping voltammetry chosen on electrochemical workstation is detected, and is obtained described in step 3)
The Stripping Voltammetry figure of malachite green solution Malachite Green；
5) step 1) is repeated to step 4), determines the Stripping Voltammetry figure of various concentration malachite green solution Malachite Green, and
The standard curve that Stripping Currents change with malachite green solution concentration is drawn out according to the voltammogram；
6) step 1) is repeated to the Stripping Voltammetry figure for 4) measuring malachite green solution Malachite Green to be measured, according to the voltammogram
Stripping Currents are obtained, using standard curve described in step 5), obtain the concentration of malachite green solution to be measured.
2. malachite green fast detecting method according to claim 1, which is characterized in that the acetate buffer solution is
The acetum of acetate, pH value 4.0-5.0.
3. malachite green fast detecting method according to claim 2, which is characterized in that in the step 1), sulfurous acid
The molar ratio of sodium or sodium thiosulfate and acetate in acetate buffer solution is 0.002:1.
4. malachite green fast detecting method according to claim 3, which is characterized in that the step 1) is by 2 μ L concentration
For 0.1mol/L sodium sulfite solution or hypo solution be added to a concentration of 0.1mol/L of 1mL acetate salt buffer it is molten
In liquid, it is uniformly mixed, obtains the sensitizer mixed liquor.
5. malachite green fast detecting method according to claim 1, which is characterized in that the electrode is silk-screen printing electricity
Pole.
6. malachite green fast detecting method according to claim 5, which is characterized in that the screen printing electrode is three
Electrode screen printing electrode plate.
7. malachite green fast detecting method according to claim 1, which is characterized in that the anodic stripping voltammetry is
Square wave anodic stripping voltammetry method.
8. malachite green fast detecting method according to claim 1, which is characterized in that in the step 5) and step 6)
Malachite green solution concentration is followed successively by 36ppb, 72ppb, 108ppb, 144ppb, 180ppb, 225ppb, 270ppb.
9. malachite green fast detecting method according to claim 1, which is characterized in that during the electrode activation,
Detect current potential 0.3V -0.7V, voltage increment 4mV, amplitude 0.025V, frequency 15Hz.
10. malachite green fast detecting method according to claim 1, which is characterized in that choose electricity in the step 4)
When anodic stripping voltammetry on chem workstation is detected, current potential 0.3V -0.7V, voltage increment 4mV, amplitude are detected
0.025V, frequency 15Hz.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949846A (en) * | 2010-08-11 | 2011-01-19 | 昆明理工大学 | Leucomalachite green detection method |
| CN102004099A (en) * | 2010-09-17 | 2011-04-06 | 大连理工大学 | Method for detecting malachite green through electrochemiluminescence |
| CN106556593A (en) * | 2016-11-18 | 2017-04-05 | 常州大学 | A kind of method of fixing tripyridyl ruthenium and detecting malachite green through electrochemiluminescence |
| CN106596663A (en) * | 2016-12-10 | 2017-04-26 | 武汉市农业科学技术研究院农业环境安全检测研究所 | Method for rapidly detecting metformin hydrochloride |
-
2018
- 2018-01-10 CN CN201810020745.2A patent/CN108344618B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949846A (en) * | 2010-08-11 | 2011-01-19 | 昆明理工大学 | Leucomalachite green detection method |
| CN102004099A (en) * | 2010-09-17 | 2011-04-06 | 大连理工大学 | Method for detecting malachite green through electrochemiluminescence |
| CN106556593A (en) * | 2016-11-18 | 2017-04-05 | 常州大学 | A kind of method of fixing tripyridyl ruthenium and detecting malachite green through electrochemiluminescence |
| CN106596663A (en) * | 2016-12-10 | 2017-04-26 | 武汉市农业科学技术研究院农业环境安全检测研究所 | Method for rapidly detecting metformin hydrochloride |
Non-Patent Citations (2)
| Title |
|---|
| DAN ZHU 等: "Determination of Malachite Green in Aquaculture Water by Adsorptive Stripping Voltammetry", 《ANALYTICAL LETTERS》 * |
| 杨小洪 等: "基于阴离子表面活性剂增敏效应的孔雀石绿伏安检测", 《湖北民族学院学报(自然科学版)》 * |
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|---|---|
| CN108344618B (en) | 2020-12-01 |
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