CN108344609A - A kind of thin layer detection method quickly differentiating true and false grape seed extract - Google Patents

A kind of thin layer detection method quickly differentiating true and false grape seed extract Download PDF

Info

Publication number
CN108344609A
CN108344609A CN201810147028.6A CN201810147028A CN108344609A CN 108344609 A CN108344609 A CN 108344609A CN 201810147028 A CN201810147028 A CN 201810147028A CN 108344609 A CN108344609 A CN 108344609A
Authority
CN
China
Prior art keywords
grape seed
seed extract
extract
solution
thin layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810147028.6A
Other languages
Chinese (zh)
Inventor
高娟
徐沛沛
惠玉虎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHAANXI JIAHE PHYTOCHEM CO Ltd
Original Assignee
SHAANXI JIAHE PHYTOCHEM CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHAANXI JIAHE PHYTOCHEM CO Ltd filed Critical SHAANXI JIAHE PHYTOCHEM CO Ltd
Priority to CN201810147028.6A priority Critical patent/CN108344609A/en
Publication of CN108344609A publication Critical patent/CN108344609A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour

Abstract

The present invention relates to plant extracts detection technique fields, the problem of whether grape seed extract is doped with pine bark, catechu and peanut coat cannot be distinguished for existing detection method, and a kind of thin layer detection method quickly differentiating true and false grape seed extract is provided, steps are as follows：Step 1：It takes grape seed extract, peanut coat extract, Catechu extract, Pine Bark and grape seed extract to be identified to pulverize and sieve, adds ethyl alcohol to mix, ultrasonic extraction obtains extracting solution；Step 2：Reference substance solution is made in the extracting solution of grape seed extract, peanut coat extract, Catechu extract, Pine Bark respectively；Detection sample solution is made in the extracting solution of grape seed extract to be identified；Step 3：By reference substance solution and detection sample solution using toluene-ethyl acetate-formic acid mixed liquor as solvent, volume ratio is 3~8：2~6：0.2~0.8, thin-layer developing after presaturation；Step 4：It is developed the color using ethanol solution of sulfuric acid, is placed under ultraviolet light and inspects；Judge.

Description

A kind of thin layer detection method quickly differentiating true and false grape seed extract
Technical field
The present invention relates to plant extracts detection technique fields, and in particular to one kind quickly differentiating true and false grape seed extract Thin layer detection method.
Background technology
Grape seed extract is that isolated a kind of polyphenols is extracted from grape pip, mainly by procyanidine, Catechin, epicatechin, gallic acid, the material compositions such as L-Epicatechin gallate.
Grape seed extract is pure natural substance, is one of the most efficient antioxidant of plant origin found so far, examination It tests and shows 30~50 times that its antioxidant effect is vitamin C and vitamin E.Procyanidine therein has extremely strong activity, It can inhibit the carcinogenic substance in cigarette, be 2~7 times of general antioxidant to the capturing ability of free radical in water phase, such as than α- The activity high twice or more of tocopherol.
Grape seed extract can effectively remove free radical extra in human body, have superpower anti-aging and enhancing immune The effect of power.Meanwhile also have the effects that antiallergy, it is antifatigue, build up health, slow down aging, for improve irritable, head Confused weak, failure of memory, improvement sub-health state etc. also have good effect.
The study found that in numerous plant tissues, catechu, peanut coat all contains in grape pip and Pine Bark The content highest of same polyphenols, wherein grape pip and Pine Bark procyanidins, it can reach 80~ 130%.
In view of grape seed extract production cost the problems such as, have a large amount of grape seed extract in the market to reach The requirement of procyanidine and polyphenol content, usually in grape seed extract mixed with pine bark, catechu or peanut coat extract, this Prodigious influence is caused on grape seed extract market.Simultaneously as pine bark, catechu, peanut coat to the effect of human body still It is unclear, it uses these to adulterate the grape seed extract of these impurity rashly, people's health may be caused potentially to endanger Evil.
Detection for active ingredient in grape pip has following several method at present：
1, Bate-Smith methods
What this method measured is the procyanidine in grape seed extract, content procyanidine index (procyanidolic index) is indicated.Due to the standard items currently without procyanidine, what the method measured is The relative value of Procyanidin in Grape Seed Extracts.The Procyanidin in Grape Seed Extracts index measured with Bate-Smith methods Generally between 80~100,100 may also be more than sometimes.
2, Porter methods
What the method measured is also the relative amount of procyanidine, as a result PVU (porter value unit) is used to indicate. But porter methods cannot distinguish between which kind of procyanidine measure be.
3, Folin-Ciocalteau methods
What this method measured is the content of total polyphenols in grape seed extract, generally with gallic acid product as a contrast.This side The advantages of method is the content of total polyphenols in energy quantitative analysis extract.The disadvantage is that:It cannot distinguish between type (such as the measurement of polyphenol It is monomer or condensate)；In addition protein, nucleic acid, the substance that ascorbic acid etc. is easily aoxidized also assist in this reaction, can be right Testing result impacts.
4, vanillic aldehyde detection method (Vanillin assay)
What this method measured is the content of flavan-3-alcohol in grape seed extract, but cannot distinguish between monomer therein and polymerization Body.Generally go to quantitative determine as standard items with catechin.
5, HPLC methods
What HPLC methods measured is each monomer in extract, oligomer, the content of high polymer.But due to the limitation of reference substance And the restriction of analysis means, the content of monomer and certain oligomers in grape seed extract can only be measured at present.
Due to grape pip, pine bark, catechu, peanut coat extract all contains similar polyphenols, and above this A little methods cannot all detect the characteristic chemical constituent in these extracts, although therefore these methods can detect grape pip Central Plains The height of anthocyanidin or polyphenol content, but cannot distinguish whether grape seed extract is doped with pine bark, catechu or peanut coat.
Invention content
The purpose of the present invention is overcome existing detection method that cannot distinguish whether grape seed extract is doped with pine bark, catechu And the problem of peanut coat, and a kind of thin layer detection method quickly differentiating true and false grape seed extract is provided.
To achieve the above object, technical solution provided by the invention is：It is a kind of quickly to differentiate true and false grape seed extract Thin layer detection method is characterized in that, steps are as follows：
Step 1：Appropriate grape seed extract, peanut coat extract, Catechu extract, Pine Bark are taken respectively and are waited for Differentiate that grape seed extract pulverizes and sieves, plus ethanol in proper amount mixing, ultrasonic extraction obtain extracting solution；
Step 2：Respectively by grape seed extract, the extracting solution of peanut coat extract, Catechu extract, Pine Bark Filtering, is made reference substance solution；
The extracting solution of grape seed extract to be identified is filtered, detection sample solution is made；
Step 3：By reference substance solution and detection sample solution point sample in same thin layer silica gel plate, with toluene-acetic acid second Ester-formic acid mixed liquor be solvent, toluene, ethyl acetate, formic acid volume ratio be 3~8：2~6：0.2~0.8, presaturation Thin-layer developing afterwards；
Step 4：After expansion, thin layer silica gel plate is taken out, is dried, is developed the color and toasted using ethanol solution of sulfuric acid, It is placed under ultraviolet light and inspects；
Judge：
Such as at identical Rf value Rf, detection sample solution has the reference substance solution same color with Catechu extract Spot, then it is assumed that the grape seed extract to be identified be mixed with catechu grape seed extract；
Such as at identical Rf value Rf, detection sample solution has and peanut coat extract reference substance solution same color Spot, then it is assumed that the grape seed extract to be identified be mixed with peanut coat grape seed extract；
Such as at identical Rf value Rf, detection sample solution has and Pine Bark reference substance solution same color Spot, then it is assumed that the grape seed extract to be identified be mixed with pine bark grape seed extract；
Such as at identical Rf value Rf, detection sample solution only has face identical as grape seed extract reference substance solution The spot of color, then it is assumed that the grape seed extract to be identified is genuine grape seed extract.
Further, in step 3, toluene, ethyl acetate, formic acid volume ratio be 3：2：0.2.
Further, in step 3, toluene, ethyl acetate, formic acid volume ratio be 5：4：0.6.
Further, in step 3, toluene, ethyl acetate, formic acid volume ratio be 8：6：0.8.
Further, in step 1, add the ethyl alcohol mixing of 6~10 times of 70% volume fractions of amount.
Further, in step 3, presaturation 15~20 minutes, the exhibition of thin-layer developing is away from for 8~10cm, this position spot Separating degree effect is good, does not overlap phenomenon.
Further, it in step 4, is developed the color using the ethanol solution of sulfuric acid of mass fraction 5%~10%, it can be multi-party Face multi-angle comparison, makes each extract characteristic spots protrude, information is comprehensive, keeps adulterated extract readily identified and recognizes.
Further, in step 4, baking temperature is 105 DEG C, 3~5 minutes time；Baking is placed under 365nm ultraviolet lights It inspects.
Compared with prior art, it is an advantage of the invention that：
1, the present invention provides a kind of method quickly differentiating true and false grape seed extract, passes through grape pip on thin layer, pine Bark, peanut coat, the respective distinctive thin layer spot of catechu distinguish it well.This method is simple, quickly, sensitivity Height, specificity are strong, at low cost, and whether to apparent mixed with the comparison of other substances in grape seed extract, visual result is easily recognized And differentiation, to ensure that the true and false property of grape seed extract provides reliable detection method.
2, the present invention use toluene-ethyl acetate-formic acid mixed liquor for solvent, toluene, ethyl acetate, formic acid body Product is than being 3~8：2~6：0.2~0.8, thin-layer developing；Spot good separating effect, each sample characteristic spots protrude, and do not have spot Point hangover and spot diffusion phenomena, Comparative result is apparent, easily determines.
3, the method that the present invention is developed the color using 5%~10% ethanol solution of sulfuric acid, many-sided multi-angle comparison, makes each extraction Object characteristic spots protrude, and information is comprehensive, keeps adulterated extract readily identified and recognizes.
4, the lamellae development distance of this method is 8~10cm, this position spot separating degree effect is good, is not overlapped existing As.
5, the present invention selects suitable ethyl alcohol to be directly ultrasonically treated, filtered, and filtrate, which is used as, detects solution, before sample Reason is simple.
Description of the drawings
Fig. 1 is the thin layer collection of illustrative plates of the reference substance solution and detection sample solution of the embodiment of the present invention 1；
Fig. 2 is the thin layer collection of illustrative plates of the reference substance solution and detection sample solution of the embodiment of the present invention 2；
Fig. 3 is the thin layer collection of illustrative plates of the reference substance solution and detection sample solution of the embodiment of the present invention 3.
Specific implementation mode
The present invention is described in further detail with reference to the accompanying drawings and examples.
Embodiment one
A kind of thin layer detection method quickly differentiating true and false grape seed extract, steps are as follows：
Step 1：Suitable grape seed raw material that crushed 80 mesh, grape seed extract, peanut coat extract, youngster are taken respectively Tea extraction, Pine Bark and three groups of grape seed extracts to be identified are placed in the triangular flask with plug, add 6 times of amounts 70% Ethyl alcohol, ultrasonic extraction obtains extracting solution in 30 minutes.
Step 2：Grape seed raw material, grape seed extract, peanut coat extract, Catechu extract, pine bark are carried respectively It takes the extracting solution of object to take out to cool, filtering with microporous membrane, filtrate product solution as a contrast；
The reference substance solution of grape seed raw material is denoted as 1, and the reference substance solution of grape seed extract is denoted as 2, peanut coat extraction The reference substance solution of object is denoted as 3, and the reference substance solution of Catechu extract is denoted as 4, and the reference substance solution of Pine Bark is denoted as 5。
The extracting solution of three groups of grape seed extracts to be identified is filtered respectively, taking-up cools, filtering with microporous membrane, and filtrate is made To detect sample solution；
The detection sample solution of first group of grape seed extract to be identified is denoted as 6, second group of grape seed extract to be identified Detection sample solution be denoted as 7, the detection sample solution of third group grape seed extract to be identified is denoted as 8.
Step 3：Reference substance solution and detection sample solution are put respectively on same silica gel g thin-layer plate, point sample origin away from The distance of lamellae lower edge is 1.0cm.
Use toluene：Ethyl acetate：Formic acid=3：2：The proportions 20mL solvents of 0.2 volume ratio, solvent is placed in In 10 × 10cm double flute expansion cylinders.The amount of solvent should be consistent in two slots.Expansion cylinder is sealed, with solvent by expansion cylinder presaturation 15 minutes.
Lamellae is put into the expansion cylinder of saturation rapidly, so that lamellae is leant against in expansion cylinder, and in expansion cylinder Solvent cannot flood point sample origin.Solvent is along lamellae ascending development, until the forward position of solvent is to point sample origin 8cm's When lamellae is taken out.
Step 4：With the solvent on hair-dryer drying lamellae, lamellae is taken out, is sprayed with 10% sulfuric acid second with spray bottle After alcoholic solution colour developing, it is placed on electric boiling plate, is heated 3~5 minutes at 105 DEG C.It cools, is inspected under 365nm ultraviolet lights.
As shown in Figure 1,
Reference substance solution 2 is consistent with the thin layer spot of reference substance solution 1, therefore reference substance solution 2 is strictly genuine grape Seed extracts.
Detect sample solution 6 near Rf=0.8 and Rf=0.7 respectively there are one with 3 same color of reference substance solution Spot, the i.e. grape seed extract to be identified are the grape seed extract mixed with peanut coat.
Detect sample solution 7 near Rf=0.2, Rf=0.55 and Rf=0.65 respectively there are one with reference substance solution 4 The spot of same color, the i.e. grape seed extract to be identified are the grape seed extract mixed with catechu.
Detect sample solution 8 near Rf=0.02 and Rf=0.45 respectively there are one with 5 same color of reference substance solution Spot, i.e., the grape seed extract to be identified be mixed with pine bark grape seed extract.
As described above, on lamellae, genuine grape pip and adulterated grape pip thin layer speckle regions are shown clearly.
Embodiment two
A kind of thin layer detection method quickly differentiating true and false grape seed extract, steps are as follows：
Step 1：Suitable grape seed raw material that crushed 80 mesh, grape seed extract, peanut coat extract, youngster are taken respectively Tea extraction, Pine Bark and three groups of grape seed extracts to be identified are placed in the triangular flask with plug, add 6 times of amounts 70% Ethyl alcohol, ultrasonic extraction obtains extracting solution in 30 minutes.
Step 2：Grape seed raw material, grape seed extract, peanut coat extract, Catechu extract, pine bark are carried respectively It takes the extracting solution of object to take out to cool, filtering with microporous membrane, filtrate product solution as a contrast；
The reference substance solution of grape seed raw material is denoted as 1, and the reference substance solution of grape seed extract is denoted as 2, peanut coat extraction The reference substance solution of object is denoted as 3, and the reference substance solution of Catechu extract is denoted as 4, and the reference substance solution of Pine Bark is denoted as 5。
The extracting solution of three groups of grape seed extracts to be identified is filtered respectively, taking-up cools, filtering with microporous membrane, and filtrate is made To detect sample solution；
The detection sample solution of first group of grape seed extract to be identified is denoted as 6, second group of grape seed extract to be identified Detection sample solution be denoted as 7, the detection sample solution of third group grape seed extract to be identified is denoted as 8.
Step 3：Reference substance solution and detection sample solution are put respectively on same silica gel g thin-layer plate, point sample origin away from The distance of lamellae lower edge is 1.0cm.
Use toluene：Ethyl acetate：Formic acid=5：4：The proportions 20mL solvents of 0.6 volume ratio, solvent is placed in In 10 × 10cm double flute expansion cylinders.The amount of solvent should be consistent in two slots.Expansion cylinder is sealed, with solvent by expansion cylinder presaturation 15 minutes.
Lamellae is put into the expansion cylinder of saturation rapidly, so that lamellae is leant against in expansion cylinder, and in expansion cylinder Solvent cannot flood point sample origin.Solvent is along lamellae ascending development, until the forward position of solvent is to point sample origin 8cm's When lamellae is taken out.
Step 4：With the solvent on hair-dryer drying lamellae, lamellae is taken out, is sprayed with 10% sulfuric acid second with spray bottle After alcoholic solution colour developing, it is placed on electric boiling plate, is heated 3~5 minutes at 105 DEG C.It cools, is inspected under 365nm ultraviolet lights.
As shown in Fig. 2,
Reference substance solution 2 is consistent with the thin layer spot of reference substance solution 1, therefore reference substance solution 2 is strictly genuine grape Seed extracts.
Detect sample solution 6 near Rf=0.75 and Rf=0.85 respectively there are one with 3 same color of reference substance solution Spot, i.e., the grape seed extract to be identified be mixed with peanut coat grape seed extract.
Detect sample solution 7 near Rf=0.18, Rf=0.6 and Rf=0.7 respectively there are one with 4 phase of reference substance solution With the spot of color, i.e., the grape seed extract to be identified is the grape seed extract mixed with catechu.
Detect sample solution 8 near Rf=0.02 and Rf=0.45 respectively there are one with 5 same color of reference substance solution Spot, i.e., the grape seed extract to be identified be mixed with pine bark grape seed extract.
As described above, on lamellae, genuine grape pip and adulterated grape pip thin layer speckle regions are shown clearly.
Embodiment three
A kind of thin layer detection method quickly differentiating true and false grape seed extract, steps are as follows：
Step 1：Suitable grape seed raw material that crushed 80 mesh, grape seed extract, peanut coat extract, youngster are taken respectively Tea extraction, Pine Bark and three groups of grape seed extracts to be identified are placed in the triangular flask with plug, add 6 times of amounts 70% Ethyl alcohol, ultrasonic extraction obtains extracting solution in 30 minutes.
Step 2：Grape seed raw material, grape seed extract, peanut coat extract, Catechu extract, pine bark are carried respectively It takes the extracting solution of object to take out to cool, filtering with microporous membrane, filtrate product solution as a contrast；
The reference substance solution of grape seed raw material is denoted as 1, and the reference substance solution of grape seed extract is denoted as 2, peanut coat extraction The reference substance solution of object is denoted as 3, and the reference substance solution of Catechu extract is denoted as 4, and the reference substance solution of Pine Bark is denoted as 5。
The extracting solution of three groups of grape seed extracts to be identified is filtered respectively, taking-up cools, filtering with microporous membrane, and filtrate is made To detect sample solution；
The detection sample solution of first group of grape seed extract to be identified is denoted as 6, second group of grape seed extract to be identified Detection sample solution be denoted as 7, the detection sample solution of third group grape seed extract to be identified is denoted as 8.
Step 3：Reference substance solution and detection sample solution are put respectively on same silica gel g thin-layer plate, point sample origin away from The distance of lamellae lower edge is 1.0cm.
Use toluene：Ethyl acetate：Formic acid=8：6：The proportions 20mL solvents of 0.8 volume ratio, solvent is placed in In 10 × 10cm double flute expansion cylinders.The amount of solvent should be consistent in two slots.Expansion cylinder is sealed, with solvent by expansion cylinder presaturation 15 minutes.
Lamellae is put into the expansion cylinder of saturation rapidly, so that lamellae is leant against in expansion cylinder, and in expansion cylinder Solvent cannot flood point sample origin.Solvent is along lamellae ascending development, until the forward position of solvent is to point sample origin 8cm's When lamellae is taken out.
Step 4：With the solvent on hair-dryer drying lamellae, lamellae is taken out, is sprayed with 10% sulfuric acid second with spray bottle After alcoholic solution colour developing, it is placed on electric boiling plate, is heated 3~5 minutes at 105 DEG C.It cools, is inspected under 365nm ultraviolet lights.
As shown in figure 3,
Reference substance solution 2 is consistent with the thin layer spot of reference substance solution 1, therefore reference substance solution 2 is strictly genuine grape Seed extracts.
Detect sample solution 6 near Rf=0.77 and Rf=0.89 respectively there are one with 3 same color of reference substance solution Spot, i.e., the grape seed extract to be identified be mixed with peanut coat grape seed extract.
Detect sample solution 7 near Rf=0.18, Rf=0.6 and Rf=0.7 respectively there are one with 4 phase of reference substance solution With the spot of color, i.e., the grape seed extract to be identified is the grape seed extract mixed with catechu.
Detect sample solution 8 near Rf=0.02 and Rf=0.45 respectively there are one with 5 same color of reference substance solution Spot, i.e., the grape seed extract to be identified be mixed with pine bark grape seed extract.
As described above, on lamellae, genuine grape pip and adulterated grape pip thin layer speckle regions are shown clearly.
Grape seed raw material is taken in above-described embodiment, you can to ensure grape seed extract authenticity as a contrast, together When can also be as the comparison of adulterant (peanut coat, catechu, pine bark) characteristic spots.
Can also exist in a grape pip sample simultaneously mixed with two or three adulterant (peanut coat, youngster in reality Tea, pine bark) the case where.It is only mixed with a kind of carrying for adulterant in three groups of grape seed extract samples to be identified in embodiment Object is taken, the representative embodiment of each adulterant is intended only as.As long as in detection sample including the feature of any one adulterant Spot, we can be judged according to the characteristic spots of adulterant its mixed with adulterant (peanut coat, catechu, pine bark) product Kind.
It the above is only and the preferred embodiment of the present invention is described, technical scheme of the present invention is not limited to This, those skilled in the art on the basis of major technique of the present invention is conceived made by any known deformation belong to institute of the present invention Technology scope to be protected.

Claims (8)

1. a kind of thin layer detection method quickly differentiating true and false grape seed extract, which is characterized in that steps are as follows：
Step 1：Appropriate grape seed extract, peanut coat extract, Catechu extract, Pine Bark and to be identified are taken respectively Grape seed extract pulverizes and sieves, and plus ethanol in proper amount mixing, ultrasonic extraction obtain extracting solution；
Step 2：Respectively by the extracting solution mistake of grape seed extract, peanut coat extract, Catechu extract, Pine Bark Filter, is made reference substance solution；
The extracting solution of grape seed extract to be identified is filtered, detection sample solution is made；
Step 3：By reference substance solution and detection sample solution point sample in same thin layer silica gel plate, with toluene-ethyl acetate- Formic acid mixed liquor be solvent, toluene, ethyl acetate, formic acid volume ratio be 3~8：2~6：0.2~0.8, it is thin after presaturation Layer expansion；
Step 4：After expansion, thin layer silica gel plate is taken out, dries, is developed the color and toasted using ethanol solution of sulfuric acid, be placed in It is inspected under ultraviolet light；
Judge：
Such as at identical Rf value Rf, detection sample solution has the spot with the reference substance solution same color of Catechu extract Point, then it is assumed that the grape seed extract to be identified is the grape seed extract mixed with catechu；
Such as at identical Rf value Rf, detection sample solution has the spot with peanut coat extract reference substance solution same color Point, then it is assumed that the grape seed extract to be identified is the grape seed extract mixed with peanut coat；
Such as at identical Rf value Rf, detection sample solution has the spot with Pine Bark reference substance solution same color Point, then it is assumed that the grape seed extract to be identified is the grape seed extract mixed with pine bark；
Such as at identical Rf value Rf, detection sample solution only has and grape seed extract reference substance solution same color Spot, then it is assumed that the grape seed extract to be identified is genuine grape seed extract.
2. the thin layer detection method according to claim 1 for quickly differentiating true and false grape seed extract, it is characterised in that：Step In rapid 3, toluene, ethyl acetate, formic acid volume ratio be 3：2：0.2.
3. the thin layer detection method according to claim 1 for quickly differentiating true and false grape seed extract, it is characterised in that：Step In rapid 3, toluene, ethyl acetate, formic acid volume ratio be 5：4：0.6.
4. the thin layer detection method according to claim 1 for quickly differentiating true and false grape seed extract, it is characterised in that：Step In rapid 3, toluene, ethyl acetate, formic acid volume ratio be 8：6：0.8.
5. the thin layer detection method according to any one of claims 1 to 4 for quickly differentiating true and false grape seed extract, feature It is：In step 1, add the ethyl alcohol mixing of 6~10 times of 70% volume fractions of amount.
6. the thin layer detection method according to claim 5 for quickly differentiating true and false grape seed extract, it is characterised in that：Step In rapid 3, presaturation 15~20 minutes, the exhibition of thin-layer developing is away from for 8~10cm.
7. the thin layer detection method according to claim 6 for quickly differentiating true and false grape seed extract, it is characterised in that：Step In rapid 4, developed the color using the ethanol solution of sulfuric acid of mass fraction 5%~10%.
8. the thin layer detection method according to claim 7 for quickly differentiating true and false grape seed extract, it is characterised in that：Step In rapid 4, baking temperature is 105 DEG C, 3~5 minutes time；Baking, which is placed under 365nm ultraviolet lights, inspects.
CN201810147028.6A 2018-02-12 2018-02-12 A kind of thin layer detection method quickly differentiating true and false grape seed extract Pending CN108344609A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810147028.6A CN108344609A (en) 2018-02-12 2018-02-12 A kind of thin layer detection method quickly differentiating true and false grape seed extract

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810147028.6A CN108344609A (en) 2018-02-12 2018-02-12 A kind of thin layer detection method quickly differentiating true and false grape seed extract

Publications (1)

Publication Number Publication Date
CN108344609A true CN108344609A (en) 2018-07-31

Family

ID=62959392

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810147028.6A Pending CN108344609A (en) 2018-02-12 2018-02-12 A kind of thin layer detection method quickly differentiating true and false grape seed extract

Country Status (1)

Country Link
CN (1) CN108344609A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101248057A (en) * 2005-06-29 2008-08-20 马尔斯公司 Preparation of (+)-catechin, (-)-epicatechin, (-)-catechin, (+)-epicatechin, and their 5,7,3',4'-tetra-o-benzyl analogues
CN101845035A (en) * 2009-03-24 2010-09-29 上海医药工业研究院 Method for extracting oligomeric proanthocyanidins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101248057A (en) * 2005-06-29 2008-08-20 马尔斯公司 Preparation of (+)-catechin, (-)-epicatechin, (-)-catechin, (+)-epicatechin, and their 5,7,3',4'-tetra-o-benzyl analogues
CN101845035A (en) * 2009-03-24 2010-09-29 上海医药工业研究院 Method for extracting oligomeric proanthocyanidins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
杨昌鹏等: "《生物分离技术》", 31 August 2007, 中国农业出版社 *
王传虎: "《有机化学实验》", 30 September 2016, 安徽大学出版社 *
高娟: ""快速鉴别葡萄籽提取物真伪薄层鉴别法有效提升鉴别效率"", 《实验与分析》 *

Similar Documents

Publication Publication Date Title
CN107843677B (en) Radix paeoniae rubra control extract and preparation method and application thereof
CN102706980B (en) Method utilizing HPLC to test seventeen phenol substances contained in grapes and oranges
CN105203657A (en) Spina date seed reference extract and preparation method and application thereof
US10802006B2 (en) Method for identifying grape seed extract authenticity using HPLC fingerprint spectrum
CN106645450B (en) The quality determining method of novel biochemical particles
CN105181847B (en) A kind of multi information gradient thinlayer discrimination method of Sculellaria barbata medicinal material and its water extract
Saad et al. Using pH variations to improve the discrimination of wines by 3D front face fluorescence spectroscopy associated to Independent Components Analysis
CN105241965B (en) A kind of method of on-line quick detection sample total economic value
Aslam et al. Pharmacognostical and phytochemical evaluation of Rheum emodi Wall
CN106645484A (en) Identification method of fake and shoddy meat floss products
CN102818863B (en) Method for identifying proanthocyanidins in ginkgo leaf preparation
CN108344609A (en) A kind of thin layer detection method quickly differentiating true and false grape seed extract
CN104330482A (en) Method utilizing HPLC to simultaneously measure 17 characteristic components in tea
CN107831261A (en) The quality determining method of rhizoma alismatis root bark of tree peony root of herbaceous peony preparation
CN102166264A (en) Shenshitong quality control method
CN104991032B (en) Differentiate to adulterate in Fructus Schisandrae Chinensis the thin layer chromatography of Fructus Schisandrae Sphenantherae
CN101829176A (en) Method for identifying rhizoma atractylodis macrocephalae by adopting thin layer chromatography
CN105758986B (en) A kind of thin-layer chromatography authentication method of Himalayan mayapple fruit medicinal material and its application
CN101700267B (en) Detection method of kaempferol and quercetin content of persimmon leaf
CN104713979A (en) Thin-layer identification method for Dendrobium officinale
CN102608251A (en) Quality control method for traumatic injury medicated wine
CN102085220B (en) Thin-layer identification method with fluorescence intensifier detection for traditional Chinese medicines
CN105866109A (en) Rapid detection method of phenolphthalein in weight reducing health foods, and detection card developing method
CN103543235A (en) Method for rapidly identifying uncooked and cooked rhizoma corydalis medicinal slices
CN111175416A (en) Method for simultaneously detecting 7 components in dogwood

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20180731