CN108342335A - Application of Bacillus belgii NZ-4 in promoting plant growth - Google Patents
Application of Bacillus belgii NZ-4 in promoting plant growth Download PDFInfo
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- CN108342335A CN108342335A CN201710058960.7A CN201710058960A CN108342335A CN 108342335 A CN108342335 A CN 108342335A CN 201710058960 A CN201710058960 A CN 201710058960A CN 108342335 A CN108342335 A CN 108342335A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12R2001/07—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention provides application of Bacillus beilesiensis NZ-4 in promoting plant growth, and the fermentation liquid of the strain NZ-4 can obviously promote the growth of potato plants. The Bacillus belgii NZ-4 and the microbial agent thereof have important significance for preventing and treating soil-borne diseases and continuous cropping obstacles of farmlands in China, increasing the yield of grains continuously and protecting the ecological environment of the farmlands.
Description
Technical field
The present invention relates to microbiologies and plant disease biological and ecological methods to prevent plant disease, pests, and erosion technical field, specifically, being related to Bei Laisi bacillus
Applications of the NZ-4 in promoting plant growth.
Background technology
Potato wilt is a kind of potato soil-borne disease caused by Fusarium oxysporum (Fusarium oxysporum)
Evil, widely distributed, in China, each growing area has generation.Potato wilt can cause to endanger to the entire breeding time of plant
Evil, pathogen mainly invades from the root wound of plant and Gen Mao growing points, and then destroys the vascular bundle of its root, stem and leaf,
Lead to part or whole vascular bundle browning, so that plant is absorbed and transport moisture function and lose, ultimately cause plant withered death.Closely
In the past few years, as potato staple food grain implementation, potato planting area expand year by year, the continuous cropping continuous cropping thus brought is led
It causes potato wilt increasingly to aggravate, seriously constrains the development of Potato Industry.Therefore, further investigation effectively prevention potato
The method of droop oneself become the upper major issue urgently to be resolved hurrily of current production.
Using microbial control plant fungal disease, to the safety of environment and the mankind, slow down pathogen drug resistance etc.
It is of great significance, has become the research hotspot of current control of plant disease.Australian phytopathologist in 1973
Kerr has successfully prevented plant root cancer using unwrapping wire agrobacterium (Agrobacterium radiobacter K84), hereafter
People go deep into the biological control research of plant disease successively.
Bacillus is a kind of aerobic or inclusive type anaerobism, generates the bacillus of resistance endospore, due to can
The antifungal substances such as lipopeptid class and protide are generated, there is broad-spectrum antifungal activity and good stability, it has also become important
Agriculture biocontrol microorganisms.Currently, in relation to bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and bacillus subtilis
The research of (Bacillus subtilis) is more.Bacillus subtilis has been commercially produced as biocontrol bacteria, it can
The rotten mould and Phytophthora nicotianae of melon and fruit on cucumber and tomato is controlled, while also having the function of promoting plant strain growth.The separation such as Song Song
There is stable antagonism to tobacco bacterial wilt to a bacillus amyloliquefaciens SQR11；Guo Rongjun etc. utilizes nutrient competition
Plate screening model obtain can on soybean wheat bran (SWB) culture medium fast-growth and antagonism pathogen of soybean root rot Fusarium
The bacillus subtilis B006 and B010 of oxysporum and F.solani；Lin Feng screens 37 plants to causing from peanut pod border
The inhibited Biocontrol Bacillus of cancer substance aflatoxin, wherein bacillus subtilis is sociales, totally 27 plants,
Secondly it is 5 plants of Bacillus cercus (Bacillus cereus), also 2 bacillus amyloliquefaciens, 2 thuringiensis strain gemma bars
Bacterium (Bacillus thuringiensis) and 1 bacillus licheniformis (Bacillus licheniformis).
The present invention occur from 16 potato wilts plot healthy plant rhizosphere separate 6 plants it is withered to potato
Disease of withering pathogen has the Bacillus strain of extremely strong resistance, using NZ-4 bacterial strains as main study subject, carried out to its antimicrobial spectrum
It measures, and control effect to potato wilt and its growth-promoting functions of potato plant is studied, it is intended to be horse
The biological control of bell potato droop and the exploitation of biocontrol agent provide scientific basis.
Invention content
The object of the present invention is to provide applications of the Bei Laisi bacillus NZ-4 in promoting plant growth.
In order to realize that the object of the invention, the present invention acquire root from Hebei, the Inner Mongol and 16, Guizhou potato plot
Border soil sample, isolated 288 bacillus bacterial strain.Through screening obtain 1 plant to Fusarium oxysporum potato specialized form have compared with
The bacterial strain NZ-4 of strong antagonism.According to its bacterium colony and morphological features and gyrB gene sequencings, it is identified as Bei Lai
This bacillus (Bacillus velezensis).Potato wilt Antagonistic Fungi NZ-4 has been preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, address Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the Chinese Academy of Sciences are micro-
Biological study institute, postcode 100101, deposit number preserving number CGMCC NO.13606, preservation date on January 13rd, 2017.
On LB culture medium flat plates, bacterial strain NZ-4 bacterium colonies are rounded, milky, and colony edge is irregular, there is protrusion on surface
Or fold, there is certain adhesiveness, a diameter of 5mm or so；Walnut picarp can be generated after 4d.Thalline is rod-shaped, is about 2 μm,
Dan Sheng.Gram's staining is positive (Fig. 2).
GyrB sequences (SEQ ID NO:1) analysis shows, bacterial strain NZ-4 and Bei Laisi bacillus (Bacillus
Velezensis) the similitude highest of (the NCBI numbers of logging in are DQ903176), it was initially believed that it is Bacillus velezensis
(phylogenetic tree is shown in Fig. 3).
The present invention provides applications of the Bei Laisi bacillus NZ-4 in promoting plant growth.
Plant of the present invention includes booth vegetable, such as potato etc..
The results from pot experiment test of Antagonistic Fungi NZ-4 prevention potato wilts shows Antagonistic Fungi NZ-4 to potato wilt
There are significant preventive and therapeutic effect, control effect 62.14%；And application Antagonistic Fungi NZ-4 fermentations liquid energy remarkably promotes potato plant
Growth.
Application above-mentioned, bacterial strain NZ-4 zymotic fluids stoste or stoste plant (seedling stage) potato after various concentration dilutes
Strain carries out root irrigation, or by the way of seed soaking.
Wherein, for zymotic fluid stoste after dilution, viable bacteria content is 1.0 × 105cfu/mL-1.0×107cfu/mL.It is preferred that living
Bacterial content 1.0 × 106cfu/mL。
Specially：After coming up, pours zymotic fluid or it releases thin liquid in root 20mL, company primary every 7d pouring roots
It is continuous to apply 4 weeks.The fermentative medium formula of bacterial strain NZ-4 is：NaCl10g, peptone 10g, yeast extract 5g, distilled water 1000mL
(LB culture mediums).
Fermentation culture conditions are：37 DEG C, 220rpm, 250mL shaking flasks, liquid amount 50mL.
The healthy plant rhizosphere that from 16 potato wilts plot occurs for the present invention separates withered to potato
Sick pathogen has the Bacillus strain NZ-4 of extremely strong resistance, and bacterial strain NZ-4 is also to potato anthracnose and shot hole, wheat root
The pathogens such as maize ear rot have very strong antagonism.It is significant anti-that pot experiment shows that Antagonistic Fungi NZ-4 has potato wilt
Control effect, control effect 62.14%；And application Antagonistic Fungi NZ-4 fermentations liquid energy remarkably promotes the growth of potato plant.It is short of money
Antibacterial NZ-4 and its microbial bacterial agent prevent China farmland soil-borne disease and continuous cropping obstacle, Continued Increasing Grain Yield and protect the fields
Ecological environment is of great significance.
Description of the drawings
Fig. 1 is tablet antagonism results of the bacterial strain NZ-4 of the present invention to potato wilt bacterium Y10-2；A is point in figure
It is bacterial strain NZ-4 to embrace sickle-like bacteria Y10-2, B.
Fig. 2 is the colonial morphology (left side) and nutrition thalline (right side) of bacterial strain NZ-4 of the present invention.
Fig. 3 is the antagonistic strain NZ-4gyrB gene order phylogenetic trees that the present invention is built.
Fig. 4 is influence of the bacterial strain NZ-4 zymotic fluids to potato plant height in the embodiment of the present invention 4.
Fig. 5 is the antagonistic effect for measuring bacterial strain NZ-4 to various pathogenic bacteria using opposite culture method in the embodiment of the present invention 5；
A is Colletotrichum coccodes potato anthrax bacterias in figure, and B is Streptomyces scabies potato sores
Scall bacterium, C are Cochliobolus sativas Cochliobolus sativus, and D is Alternaria kikuchiana Tanaka pears
Alternaria, E are Alternaria alternaria f.sp mali Alternaria malis, F Alternaria
Solani tomato early blight bacteriums, G are Fusarium equiseti red rot bacterium, and H is Phytophthora nicotianae cigarettes
Careless phytophthora.
Specific implementation mode
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
According to conventional laboratory conditions, as Sambrook equimoleculars Cloning: A Laboratory Manual (Sambrook J＆Russell DW,
Molecular Cloning:A Laboratory Manual, 2001), or according to the condition of manufacturer's specification suggestion.
The separation and purifying of 1 potato wilt Antagonistic Fungi NZ-4 of embodiment
1 materials and methods
1.1 soil samples acquire
From the potato wilt in Baoding, Chengde, Zhangjiakou, Qinhuangdao and the Inner Mongol, Guizhou falls ill field
The rhizosphere soil of healthy plant is acquired, sampling depth ranging from 10~20cm takes back laboratory, temporarily after being sealed with sterile valve bag
4 DEG C are stored in, for detaching Antagonistic Fungi.
1.2 for trying target pathogens
1 plant of Fusarium oxysporum (Fusarium oxysporum, Y10-2) bacterial strain is Fusarium oxysporum dominant bacteria, by this
Laboratory (Agricultural University Of Hebei's Plant Pathology laboratory) provides.
1.3 for trying culture medium
Bacterium is LB culture mediums, and fungi is PDA culture medium.
The separation and purifying of 1.4 Bacillus strains
Bacillus is detached using heat treatment soil dilution partition method, continuous scribing line culture is to pure, with 1：1 is mixed in 70%
In glycerine, -80 DEG C of refrigerators save backup.
The screening of 1.5 Antagonistic Fungis
Antagonism of the bacillus to Fusarium oxysporum Y10-2 is measured using tablet face-off method.Primary dcreening operation uses antibacterial band
Inoculation plate is placed in 25 DEG C of constant incubators and cultivates by method, and the PDA plate to be only inoculated with pathogen is to compare, basis after 5d
Antibacterial situation filters out the preferable bacterial strain of fungistatic effect, and secondary screening experiment is carried out using inhibition zone method.
2 results and analysis
The separation of 2.1 Bacillus strains and the screening of antagonistic strain
It is isolated and purified from 16 parts of rhizosphere soils to the bacillus to potato wilt bacterium with potential antagonism
There are 288 plants.188 plants of the antagonistic strain that antibacterial bandwidth >=3mm is obtained by tablet face-off method primary dcreening operation, accounts for all bacterial strains
65.3%, wherein 20 plants of antibacterial bandwidth of Antagonistic Fungi reach 5.7mm or more.Continue to carry out secondary screening to 20 plants of Antagonistic Fungis, be pressed down
6 plants of the Antagonistic Fungi (table 1, Fig. 1) of bacterium loop diameter >=20.0mm.
1 isolated strains of table are to potato wilt bacterium growth inhibition effect
Note：With after column data, different letters indicate difference up to 5% level of signifiance
The identification of 2 antagonistic strain of embodiment
The antagonistic strain screened is carried out with the method that molecular biology is combined using colonial morphology, Gram's staining
Identification.
The sequence analysis of gyrB genes：GyrB gene magnification primer sequences[15]Forward primer gyrB-F：5'-
TTGRCGGHRGYGGHTATAAAGT-3'；Reverse primer gyrB-R：5'-TCCDCCSTCAGARTCWCCCTC-3'.PCR amplification
Program is：95 DEG C of pre-degeneration 3min；94 DEG C of denaturation 30s, 55 DEG C of annealing 45s, 72 DEG C of extension 1min, 35 recycle；Last 72 DEG C
Extend 10min, 4 DEG C terminate reaction.PCR product on 1.5% agarose gel electrophoresis detect after, send raw work bioengineering (on
Sea) Co., Ltd is sequenced.Row will be sequenced, BLAST comparisons are carried out by ncbi database, utilizes Clustalx and MEGA
6.0 softwares carry out Phylogenetic Analysis to separation bacillus and pattern bacterium.
On LB culture medium flat plates, bacterial strain NZ-4 bacterium colonies are rounded, milky, and colony edge is irregular, there is protrusion on surface
Or fold, there is certain adhesiveness, a diameter of 5mm or so；Walnut picarp can be generated after 4d.Thalline is rod-shaped, is about 2 μm,
Dan Sheng.Gram's staining is positive (Fig. 2).
GyrB sequences (SEQ ID NO:1) analysis shows, bacterial strain NZ-4 and Bei Laisi bacillus (Bacillus
Velezensis) the similitude highest of (the NCBI numbers of logging in are DQ903176), it was initially believed that it is Bacillus velezensis
(phylogenetic tree is shown in Fig. 3).
3 Antagonistic Fungi of embodiment prevents the pot experiment of potato wilt
Using leaching root method with 1.0 × 108Cfu/mL Antagonistic Fungi fermentation liquor treatment 2h are control with clear water processing, often handle
30 plants, amount to 60 plants；After for 24 hours, with 1 × 106The Fusarium oxysporum Y10-2 spore suspensions of a/mL soak root 30min, after being inoculated with germ
Potato plant set handled under dark conditions for 24 hours, keep ambient humidity be 95%, to promote germ to infect.It is transferred to afterwards for 24 hours
Normal lighting conditions (14h illumination:10h is dark) culture.Potato incidence is investigated after 20d, and using conventional tissue point
The separation that germ is carried out to disease plant from method is identified.Disease index is calculated with reference to pathogenicities grade scales such as Krumholz.
Disease index：(DI)=∑ (the sick grade × disease grade strain number)/(highest disease grade × investigation strain number) × 100
Results from pot experiment test shows that the incidence of the potato plant after antagonistic strain NZ-4 fermentation liquor treatments after 20d is
56.67%, less than control (clear water processing) incidence 83.3%, treated that plant disease index is by antagonistic strain NZ-4
26.00, far below the disease index 68.67 of control, control effect reaches 62.14%.Illustrate antagonistic strain NZ-4 zymotic fluids pair
Potato wilt has good control effect.
Influence of the 4 Antagonistic Fungi NZ-4 zymotic fluids of embodiment to potato plant plant height
Biological control is as follows：Use method for soaking seed respectively with 1.0 × 108Cfu/mL bacterial strains NZ-4 (stoste) and 1.0 ×
106Cfu/mL (100 times of dilutions) handles potato potato wedge, is stored at room temperature 2h, removes bacterial strain fermentation liquor, waits for that natural air drying is laggard
Row sowing.Stoste and 100 times of dilutions are poured after 7d respectively again in root 20mL, are control with clear water.It is primary every 7d pouring roots,
Potato set plant height is investigated after 4 weeks.
Root irrigation is carried out to seedling stage potato plant with the NZ-4 bacterial strain fermentation liquors of various concentration, clear water does blank pair
According to observing and count growing state of plant under the conditions of different disposal.The results show that handling 4 through NZ-4 strain fermentation stostes
The average plant height of plant after week is 72.58mm, higher than the processing plant height 62.66mm and control plant height diluted after 100 times
(60.87mm Fig. 4).Thus, NZ-4 bacterial strain fermentation liquors have facilitation to the growth of potato plant.
The fermentative medium formula of bacterial strain NZ-4 is：NaCl 10g, peptone 10g, yeast extract 5g, distilled water 1000mL
(LB culture mediums).
Fermentation culture conditions are：37 DEG C, 220rpm, 250mL shaking flasks, liquid amount 50mL.
5 Antagonistic Fungi antimicrobial spectrum of embodiment measures
Antagonistic strain is measured to endangering the antibacterial of serious 8 plants of plant pathogenic fungis in agricultural production using opposite culture method
Effect.
NZ-4 pairs of 8 pathogen strain bacterial strains of bacterial strain are subjected to antimicrobial spectrum measurement.The results show that bacterial strain NZ-4 is to potato anthracnose
Best with the fungistatic effect of shot hole, antibacterial circle diameter reaches 35mm；To Cochliobolus sativus, Pear black spot bacterium, apple spot
The antibacterial circle diameter of fallen leaves germ and tomato early blight bacterium is in 24mm or more；Also certain antagonism makees red rot bacterium
With；But to the antagonism unobvious of Phytophthora nicotianae Breda (table 2 and Fig. 5).Illustrate the antimicrobial spectrum that bacterial strain NZ-4 has comparison wide, it can be with
Consider the biological and ecological methods to prevent plant disease, pests, and erosion research for other crop diseases being applied in addition to potato wilt.
2 antagonistic strain NZ-4 of table is to various pathogenic bacteria antagonistic effect
Note：With after column data, different letters indicate difference up to 5% level of signifiance
The researchs such as Song Song find that the part bacterial strain of soil separation shows stronger bacteriostasis in the starting stage, with bacterium
The proliferation of body quantity, bacteriostasis can occur largely weak.Bacillus due to its can generate with high temperature resistant,
The strong gemma of resistance so that such bacterial strain can steadily in the long term survive in the living environment of rather harsh, and can be in item
Part sprouts rapidly proliferation suitable in the case of, and continuous release antibacterial substance inhibits a variety of pathogens.The present invention is thin in soil
Soil Slurry heat treating process is used in bacterium screening process, although this method can kill auxotype gemma bar during heating
Bacterium reduces measurement result；But since heat treatment kills non-bacillus, bacillus can be purposefully screened, meanwhile, it fits
It is that screening Biocontrol Bacillus is concisely and efficiently method to simplify separation and appraisal when reducing dilution.
The antagonistic strain NZ-4 that the present invention is screened through dual test shows stronger inhibition in greenhouse pot culture experiment
Effect.
The microenvironment that microbial bacterial agent is survived by improving or creating suitable Biocontrol microorganism, improves it and is fitted to environment
Should be able to power, to improve its biocontrol effect.Mainly from humidity, production technology and carrier etc. in the development process of microbial bacterial agent
Aspect improves its storage stability.Tian Xiaoli determines wheat bran+corn flour (5 through screening:3) it is solid state rheology matrix, with kaolinite
Soil is carrier, and biocontrol actinomycetes F104 is combined with non-pathogenic Fusarium oxysporum FO47, compounding microbial inoculum is made, greatly improves
The biological preventive effect of FO47.It would therefore be desirable to microbial bacterial agent further is made in NZ-4 bacterial strain fermentation liquors, also, it is stringent
Design later stage pot experiment, control effect of the systematicness verification NZ-4 bacterial strains to potato wilt.
The Antagonizing of bacillus, which is often as it, can generate bioactive substance.The antagonist that bacillus generates
Matter mainly has bacteriocin, antibiotic, Lysozyme, other Antagonistic proteins and volatility antibacterial material etc..Lipopeptid class antibiosis
Element is the main antibacterial substance that bacillus generates, some in these substances are only anti-same or similar to have specific receptors site
Type.Result of study shows that bacterial strain NZ-4 also can to a variety of Important Agricultural disease pathogens in addition to potato wilt
Stronger antagonism is generated, is thus speculated, there may be a variety of different antibacterial substances or the bacterial strain to generate by bacterial strain NZ-4
Antibacterial substance there is wider bacteriostasis, specific active material ingredients still to need further to be studied.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
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Sequence table
<110>Agricultural University Of Hebei
<120>Applications of the Bei Laisi bacillus NZ-4 in promoting plant growth
<130> KHP171110081.0TQ
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 940
<212> DNA
<213>Bei Laisi bacillus（Bacillus velezensis）
<400> 1
acggtgtagg ggcgtccgtc gtaaacgcct tgtcgaccac tcttgacgtt acggttcatc 60
gtgacggaaa aatccactat caggcgtacg agcgcggtgt gcctgtggcc gatcttgaag 120
tgatcggtga tactgataag accggaacga ttacgcactt cgttccggat ccggaaatct 180
tcaaagaaac aactgtatac gactatgatc tgctttcaaa ccgtgtccgg gaattggcct 240
tcctgacaaa aggcgtaaac atcacgattg aagacaaacg tgaaggacaa gaacggaaaa 300
acgagtacca ctacgaaggc ggaatcaaaa gctatgttga gtacttaaac cgttccaaag 360
aagtcgttca tgaagagccg atttatatcg aaggcgagaa agacggcata acggttgaag 420
ttgcattgca atacaacgac agctatacaa gcaatattta ttctttcaca aataatatca 480
acacatacga aggcgggacg cacgaggccg gatttaaaac tggtctgacc cgtgtcataa 540
acgactatgc aagaagaaaa gggattttca aagaaaatga tccgaattta agcggggatg 600
atgtgagaga agggctgact gccattattt caattaagca ccctgatccg caattcgaag 660
ggcagacgaa aaccaagctc ggcaactctg aagcgagaac gatcactgac acgctgtttt 720
cttctgcgct ggaaacattc cttcttgaaa atccggactc agcccgcaaa atcgttgaaa 780
aaggtttaat ggccgcaaga gcgcggatgg cagcgaaaaa agcgcgggaa ttgacccggc 840
gcaaaagtgc gcttgagatt tccaatctgc cgggcaaact ggcggactgt tcttctaaag 900
atccgagcat ttccgagctt gtatatcgta gagggagatc 940
Claims (8)
1. applications of the Bei Laisi bacillus NZ-4 in promoting plant growth, wherein the Bei Laisi bacillus
The preserving number of (Bacillus velezensis) NZ-4 is CGMCC NO.13606.
2. application according to claim 1, which is characterized in that the plant includes booth vegetable.
3. application according to claim 2, which is characterized in that the plant is potato.
4. application according to claim 3, which is characterized in that bacterial strain NZ-4 zymotic fluids stoste or stoste are dilute through various concentration
After releasing, root irrigation is carried out to potato plant.
5. application according to claim 4, which is characterized in that after coming up, pour zymotic fluid or its release thin liquid in
Root 20mL, continuous administration 4 week primary every 7d pouring roots.
6. application according to claim 3, which is characterized in that bacterial strain NZ-4 zymotic fluids stoste or stoste are dilute through various concentration
After releasing, seed soaking is carried out.
7. the application according to claim 4 or 6, which is characterized in that zymotic fluid stoste is after dilution, viable bacteria content 1.0
×105cfu/mL-1.0×107cfu/mL。
8. application according to claim 7, which is characterized in that zymotic fluid stoste after dilution, viable bacteria content be 1.0 ×
106cfu/mL。
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| CN114058541A (en) * | 2021-11-17 | 2022-02-18 | 河北农业大学 | Pesticide-resistant and bolas-resistant biocontrol bacillus beiLeisi for wheat sheath blight and application |
| WO2023138678A1 (en) * | 2022-01-21 | 2023-07-27 | 慕恩（广州）生物科技有限公司 | Bacillus velezensis and use thereof |
| CN114540249A (en) * | 2022-03-23 | 2022-05-27 | 云南农业大学 | Antagonistic strain 1X1Y and application thereof |
| CN115044494A (en) * | 2022-04-27 | 2022-09-13 | 内蒙古自治区农牧业科学院 | Bacillus subtilis WZ10 and application thereof |
| CN115044494B (en) * | 2022-04-27 | 2023-06-23 | 内蒙古自治区农牧业科学院 | Bacillus subtilis WZ10 and application thereof |
| CN117126750A (en) * | 2023-08-30 | 2023-11-28 | 云南农业大学 | Fusarium oxysporum J2 strain and application thereof in biological prevention and control and growth promotion of pseudo-ginseng |
| CN117126750B (en) * | 2023-08-30 | 2024-07-12 | 云南农业大学 | Fusarium oxysporum J2 strain and application thereof in biological prevention and control and growth promotion of pseudo-ginseng |
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