CN108341904A - A kind of preparation method of multi-fluorescence label polystyrene microsphere - Google Patents
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- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F212/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring
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Abstract
The present invention relates to the preparation methods that a kind of multi-fluorescence marks polystyrene microsphere, include the following steps：It is polymerize by two-step seed swelling and prepares surface carboxylation polystyrene microsphere, above-mentioned polystyrene microsphere is combined with quantum dot and prepares quantum dot fluorescent microsphere, the carboxyl of quantum dot fluorescence microsphere surface is coupled with phycoerythrin, obtains the fluorescence probe polystyrene microsphere of multiple labelling.The method of the present invention is simple, low in raw material price, the polystyrene microsphere stable structure of preparation, is marked using quantum dot and phycoerythrin double fluorescent, and performance characteristics are notable, and safe preparation process coefficient is high, to human body without security risk.
Description
Technical field
The present invention relates to polymer microballoon technical field of material, and in particular to a kind of multi-fluorescence label polystyrene
The preparation method of microballoon.
Background technology
Polystyrene fluorescent microsphere is the uniform microballoon dyed with fluorescent reagent.Fluorescent microsphere refer to diameter nanoscale extremely
In micro-scaled range, load has fluorescent material, and the solia particle of fluorescence can be inspired by outside energy stimulation.Its shape can be to appoint
Meaning shape, typical shape are spherical shape.The carrier of fluorescent microsphere is mostly organic or inorganic polymer material.It has stablizes relatively
Morphosis and luminescent behavior, influenced by external condition such as solvent, heat, electricity, magnetic etc. it is smaller than pure fluorescent chemicals,
It is widely used to biomedical sector as a kind of novel carrier material.As that studies functional polymer gos deep into,
Fluorescent microsphere (Fluorescent microspheres) efficient luminous efficiency with the morphosis and stabilization of its stabilization,
There is critically important application in many fields, especially have in biological immune detection and are widely applied very much.
It is well known that polystyrene microsphere has good dispersion, large specific surface area, has biologically inert, not by common solvent
Dissolving or swelling, and have the specific physiques such as surface respond, have to substances such as protein, dyestuff, close ligands
Binding ability is suitable as the carrier of fluorescent material in bioanalysis.Existing fluorescent material exists certain in application process
Defect：1, photostability is poor, and luminous spectrum is narrow and discontinuous, and emission spectra is wider and asymmetric, and emission spectrum can not regulate and control；2, fluorescence
It is easy quenching, short life；3, a small number of fluorescent materials have certain radioactivity and toxicity, and there are security risks.
Invention content
Technical problem to be solved by the invention is to provide a kind of multi-fluorescence mark polystyrene microsphere preparation method,
This method manufacture craft is simple, and advantages of nontoxic raw materials is harmless, securely and reliably；Meanwhile it being replaced using quantum dot and phycoerythrin traditional
Fluorescent material carries out double labeling, is easily combined with other molecules, photostability enhancing, and fluorescence lifetime is long.
The technical solution that the present invention solves above-mentioned technical problem is as follows：
A kind of preparation method of multi-fluorescence label polystyrene microsphere, includes the following steps：
S1：It is polymerize by two-step seed swelling and prepares surface carboxylation polystyrene microsphere；
S2：Polystyrene microsphere in S1 is combined with quantum dot and prepares quantum dot fluorescent microsphere；
S3：The carboxyl of quantum dot fluorescence microsphere surface is coupled with phycoerythrin, obtains the fluorescence probe polyphenyl of multiple labelling
Ethylene microballoon.
The beneficial effects of the invention are as follows：Quantum dot is replaced traditional fluorescent dye, quantum dot to have good by the present invention
Photostability, width and continuous excitation spectrum, narrow and symmetrical emission spectra；Coupling phycoerythrin makees fluorescence analysis simultaneously, dual
Fluorescent marker, fluorescence lifetime is long, and fluorescence analysis effect is preferable；Production method of the present invention is simple, and advantages of nontoxic raw materials is harmless, safety coefficient
Security risk is not present to human body in height.
Based on the above technical solution, the present invention can also be improved as follows.
Further, in the present invention, the specific preparation process of surface carboxylation polystyrene microsphere is as follows in step S1：
S11：By seed microballoon and emulsifier according to 1:5~10 mass ratio mixing, is ultrasonically treated, obtains the of suspension
One swelling object；
S12：By hexamethylene and mentioned emulsifier according to 1:5~10 mass ratio mixing, is ultrasonically treated, obtains suspension
Second swelling object；
S13：It is 1 to be swollen object and the second swelling object according to mass ratio by above-mentioned first:1~1.5 is mixed, room temperature swelling
4~8h, then sequentially adds initiator, polymerized monomer, crosslinking agent and mentioned emulsifier, initiator, polymerized monomer, crosslinking agent and
The amount ratio of emulsifier is 0.1:5~10:10~20:5~10, continue 6~12h of stirring；
S14：Above-mentioned solution is transferred to 80~120 DEG C of oil bath pans, it is 2~10 to sequentially add amount ratio:0.03~
0.15:5~25 stabilizers, coloring agent and ultra-pure water react 6~18h, finally use the washing of 10~15vol% ethyl alcohol and gravity sieve
The carboxylated micro-spheres of size uniformity are obtained after choosing.
Further, the emulsifier in the step S11 is lauryl sodium sulfate (SDS).
Further, the initiator in the step S13 is dibenzoyl peroxide (BPO), and polymerized monomer is styrene, is handed over
It is divinylbenzene or toluene or acrylic acid to join agent.
Further, stabilizer is polyvinylpyrrolidone (PVP) in the step S14, and coloring agent is methylene blue.
Further, in the present invention, the specific preparation process of quantum dot fluorescence microballoon is as follows in step S2：
S21：The carboxylic polystyrene microsphere is added in dispersant, is added a concentration of 10-2~10-8mmol/L
The mass ratio of quantum dot solution, carboxylic polystyrene microsphere and quantum dot microsphere addition is 1:10-3~10-9, be ultrasonically treated into
Row is separated by solid-liquid separation, and is then dried in vacuo solvent flashing；
S22：It is 95 by the solid volume ratio in S21:5 ethyl alcohol washs 2~3 times with hexamethylene mixed liquor, and storage is extremely
In 10~1000mL ultra-pure waters, quantum dot fluorescence microballoon is obtained.
Further, the dispersant of the step S21 is chloroform and isopropyl alcohol mixture, the volume ratio of chloroform and isopropanol
It is 95:5.
Further, in the present invention, the specific preparation process that quantum dot fluorescence microballoon is coupled with phycoerythrin in step S3 is such as
Under：
S31：It takes the quantum dot fluorescence microballoon in S22 to be dispersed in buffer solution and be added coupling agent, stirs 15~20min,
Reaction solution, the mass ratio of quantum dot fluorescence microballoon, buffer solution and coupling agent is 1 in reaction solution:500~600:0.15~0.3；
S32：It is added phycoerythrin in S31 reaction solutions, quantum dot fluorescence microballoon in the phycoerythrin and reaction solution of addition
Mass ratio be 0.1~10:1,30~60 DEG C of rotation 2~4h of concussion reaction, are then washed with above-mentioned buffer solution.
Further, it is 1 that the coupling agent, which is amount ratio,:2~5 1- ethyls-(3- dimethylaminopropyls) phosphinylidyne two is sub-
Amine hydrochlorate (EDC.HCL) and n-hydroxysuccinimide (NHS) mixture.
Further, the buffer solution is 0.01mol/L PBS buffer solutions.
Specific implementation mode
Principles and features of the present invention are described below in conjunction with specific embodiment, example is served only for explaining this hair
It is bright, it is not intended to limit the scope of the present invention.
Embodiment 1
The present embodiment provides the preparation methods that a kind of multi-fluorescence marks polystyrene microsphere, include the following steps：
S1 is polymerize by two-step seed swelling and prepares surface carboxylation polystyrene microsphere：
S11：Seed microballoon 0.1g is weighed to be added in lauryl sodium sulfate (SDS) aqueous solution of 30mL0.25Wt%,
Ultrasonic disperse 20min under the conditions of 200W obtains the first swelling object；
S12：Another lauryl sodium sulfate (SDS) aqueous solution for taking 0.1g hexamethylenes to be added to 30mL0.25Wt%, 200W
Under the conditions of ultrasonic disperse 20min, obtain second swelling object；
S13：Object and the second swelling object are swollen according to 1 by above-mentioned first:1.2 ratio is mixed, and room temperature is swollen 4h, according to
Secondary addition 0.1g dibenzoyl peroxides (BPO), 20g styrene, 20g divinylbenzenes, 20g toluene, 1.0g acrylic acid and
Lauryl sodium sulfate (SDS) aqueous solution of 80mL0.25Wt% continues to stir 6h；
S14：Above-mentioned solution is transferred to 80 DEG C of oil bath pans, sequentially add 4g polyvinylpyrrolidones (PVP, K=30),
The methylene blue aqueous solution and 50mL ultra-pure waters of 5mL7.5mg/mL reacts 6h, is finally washed with 10vol% ethyl alcohol and gravity screens
Obtain the carboxylated micro-spheres of size uniformity；
Polystyrene microsphere in S1 is combined with quantum dot and prepares quantum dot fluorescent microsphere by S2：
S21：The carboxylic polystyrene microsphere is added in 50mL chloroforms and isopropyl alcohol mixture, chloroform with it is different
The volume ratio of propyl alcohol is 95:5,100mL a concentration of 10 is then added-3Mmol/L quantum dot solutions, ultrasound 10min under the conditions of 400W
After be separated by solid-liquid separation, be then dried in vacuo solvent flashing；
S22：By the 100mL ethyl alcohol of the solid in S21 and hexamethylene mixed liquor, (ethyl alcohol is 95 with hexamethylene volume ratio:5)
Washing 2~3 times in storage to 100mL ultra-pure waters, obtains quantum dot fluorescence microballoon；
S3, carboxyl and the phycoerythrin of quantum dot fluorescence microsphere surface are coupled, and obtain the fluorescence probe polyphenyl of multiple labelling
Ethylene microballoon：
S31：It takes the quantum dot fluorescence microballoon in S22 to be scattered in 5mLPBS buffer solutions, carboxyl coupling agent 6mg1- is added
Ethyl-(3- dimethylaminopropyls) phosphinylidyne diimmonium salt hydrochlorate (EDC.HCL) and 13mgN- HOSu NHSs (NHS) are mixed
Object is closed, 15min is stirred；
S32：30mg phycoerythrin, 37 DEG C of rotation concussion reaction 2h, then with above-mentioned are added in S31 reaction solutions
0.01mol/L PBS buffer solutions wash.
Embodiment 2
The present embodiment provides the preparation methods that a kind of multi-fluorescence marks polystyrene microsphere, include the following steps：
S1 is polymerize by two-step seed swelling and prepares surface carboxylation polystyrene microsphere：
S11：Seed microballoon 0.3g is weighed to be added in lauryl sodium sulfate (SDS) aqueous solution of 60mL 0.25Wt%,
Ultrasonic disperse 30min under the conditions of 300W obtains the first swelling object；
S12：Another lauryl sodium sulfate (SDS) aqueous solution for taking 0.3g hexamethylenes to be added to 60mL 0.25Wt%, 300W
Under the conditions of ultrasonic disperse 30min, obtain second swelling object；
S13：It is swollen object by above-mentioned first and the second swelling object is carried out according to 1:1.3 ratio mixing, room temperature are swollen 6h, according to
Secondary addition 0.3g dibenzoyl peroxides (BPO), 30g styrene, 30g divinylbenzenes, 30g toluene, 1.5g acrylic acid and 80mL
Lauryl sodium sulfate (SDS) aqueous solution of 0.25Wt% continues to stir 9h；
S14：Above-mentioned solution is transferred to 80 DEG C of oil bath pans, sequentially add 6g polyvinylpyrrolidones (PVP, K=30),
The methylene blue aqueous solution and 50mL ultra-pure waters of 15mL 7.5mg/mL reacts 12h, finally washs simultaneously gravity with 10vol% ethyl alcohol
Screening obtains the carboxylated micro-spheres of size uniformity；
Polystyrene microsphere in S1 is combined with quantum dot and prepares quantum dot fluorescent microsphere by S2：
S21：The carboxylic polystyrene microsphere is added in 200mL chloroforms and isopropyl alcohol mixture, chloroform with
The volume ratio of isopropanol is 95:5,500mL a concentration of 10 is then added-5Mmol/L quantum dot solutions, ultrasound under the conditions of 400W
It is separated by solid-liquid separation after 10min, is then dried in vacuo solvent flashing；
S22：By the 600mL ethyl alcohol of the solid in S21 and hexamethylene mixed liquor, (ethyl alcohol is 95 with hexamethylene volume ratio:5)
Washing 2~3 times in storage to 600mL ultra-pure waters, obtains quantum dot fluorescence microballoon；
S3, carboxyl and the phycoerythrin of quantum dot fluorescence microsphere surface are coupled, and obtain the fluorescence probe polyphenyl of multiple labelling
Ethylene microballoon：
S31：It takes the quantum dot fluorescence microballoon in S22 to be scattered in 5mL PBS buffer solutions, carboxyl coupling agent 7mg is added
1- ethyls-(3- dimethylaminopropyls) phosphinylidyne diimmonium salt hydrochlorate (EDC.HCL) and 15mg n-hydroxysuccinimides
(NHS) mixture stirs 18min；
S32：60mg phycoerythrin, 37 DEG C of rotation concussion reaction 3h, then with above-mentioned are added in S31 reaction solutions
0.01mol/L PBS buffer solutions wash.
Embodiment 3
The present embodiment provides the preparation methods that a kind of multi-fluorescence marks polystyrene microsphere, include the following steps：
S1 is polymerize by two-step seed swelling and prepares surface carboxylation polystyrene microsphere：
S11：Seed microballoon 0.5g is weighed to be added in lauryl sodium sulfate (SDS) aqueous solution of 90mL 0.25Wt%,
Ultrasonic disperse 40min under the conditions of 100~500W obtains the first swelling object；
S12：Another lauryl sodium sulfate (SDS) aqueous solution for taking 0.5g hexamethylenes to be added to 90mL 0.25Wt%, 400W
Under the conditions of ultrasonic disperse 40min, obtain second swelling object；
S13：It is swollen object by above-mentioned first and the second swelling object is carried out according to 1:1.4 ratio mixing, room temperature are swollen 8h, according to
Secondary addition 0.5g dibenzoyl peroxides (BPO), 40g styrene, 40g divinylbenzenes, 40g toluene, 2.0g acrylic acid and 80mL
Lauryl sodium sulfate (SDS) aqueous solution of 0.25Wt% continues to stir 12h；
S14：Above-mentioned solution is transferred to 80 DEG C of oil bath pans, sequentially add 8g polyvinylpyrrolidones (PVP, K=30),
The methylene blue aqueous solution and 50mL ultra-pure waters of 20mL 7.5mg/mL reacts 18h, finally washs simultaneously gravity with 10vol% ethyl alcohol
Screening obtains the carboxylated micro-spheres of size uniformity；
Polystyrene microsphere in S1 is combined with quantum dot and prepares quantum dot fluorescent microsphere by S2：
S21：The carboxylic polystyrene microsphere is added in 400mL chloroforms and isopropyl alcohol mixture, chloroform with
The volume ratio of isopropanol is 95:5,800mL a concentration of 10 is then added-7Mmol/L quantum dot solutions, ultrasound under the conditions of 400W
It is separated by solid-liquid separation after 10min, is then dried in vacuo solvent flashing；
S22：By the 900mL ethyl alcohol of the solid in S21 and hexamethylene mixed liquor, (ethyl alcohol is 95 with hexamethylene volume ratio:5)
Washing 2~3 times in storage to 800mL ultra-pure waters, obtains quantum dot fluorescence microballoon；
S3, carboxyl and the phycoerythrin of quantum dot fluorescence microsphere surface are coupled, and obtain the fluorescence probe polyphenyl of multiple labelling
Ethylene microballoon：
S31：It takes the quantum dot fluorescence microballoon in S22 to be scattered in 5mL PBS buffer solutions, carboxyl coupling agent 9mg is added
1- ethyls-(3- dimethylaminopropyls) phosphinylidyne diimmonium salt hydrochlorate (EDC.HCL) and 20mg n-hydroxysuccinimides
(NHS) mixture stirs 20min；
S32：90mg phycoerythrin, 37 DEG C of rotation concussion reaction 4h, then with above-mentioned are added in S31 reaction solutions
0.01mol/L PBS buffer solutions wash.
The present invention using quantum dot replace traditional fluorescent dye, quantum dot compared with traditional fluorescent dye,
With good photostability, width and continuous excitation spectrum, narrow and symmetrical emission spectra, larger Stokes shift and
Good biocompatibility.In contrast, the fluorescence lifetime of quantum dot is longer.It is more outstanding to be, in a certain range, quantum
Point can also realize the regulation and control of the emission spectrum of quantum dot by adjusting its size；Its dissolubility can also be repaiied according to its surface
Decorations are more flexible to realize regulation and control, regulation and control
Phycoerythrin is also used for fluorescence analysis by the present invention simultaneously, with incomparable superior of traditional chemical fluorescent dye
Property：There is wider absorption spectrum within the scope of wider pH, be easier to select suitable excitation wavelength, to obtain efficiently
Fluorescent emission, and have special fluorescence emission peak when excitation；Absorbance and fluorescence quantum yield are very high, and fluorescence is strong and stablizes, spirit
Sensitivity is high；It with smaller fluorescence background, is not easy to be quenched, fluorescence storage life is longer；Excellent aqueous solubility is easy to other molecule cross-links
In conjunction with non-specific adsorption is few；Pure-natural marine living-things extract, without any side effects, are free of radioactivity, operate with very
Safety.
In comparison, the polystyrene microsphere that the preparation method provided through the invention is prepared has double fluorescent
The characteristic of label, size uniformity, fluorescent characteristic is more notable, and fluorescence lifetime is longer, is conducive to fluorescence analysis, is especially applied to
The fluorescence analysis of nano meter biomaterial, analytical effect are notable.Polystyrene microsphere provided by the invention has wide range of applications, not only
Nano meter biomaterial field is can be applied to, biochemical sensor, optical material, biomedicine, streaming fluorescence, number are applied also for
The fields such as word medical treatment, market prospects are boundless.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of preparation method of multi-fluorescence label polystyrene microsphere, which is characterized in that include the following steps：
S1：It is polymerize by two-step seed swelling and prepares surface carboxylation polystyrene microsphere；
S2：Polystyrene microsphere in S1 is combined with quantum dot and prepares quantum dot fluorescent microsphere；
S3：The carboxyl of quantum dot fluorescence microsphere surface is coupled with phycoerythrin, obtains the fluorescence probe polystyrene of multiple labelling
Microballoon.
2. a kind of preparation method of multi-fluorescence label polystyrene microsphere according to claim 1, which is characterized in that step
The specific preparation process of surface carboxylation polystyrene microsphere is as follows in rapid S1：
S11：By seed microballoon and emulsifier according to 1:5~10 mass ratio mixing, is ultrasonically treated, obtain suspension first is molten
Swollen object；
S12：By hexamethylene and mentioned emulsifier according to 1:5~10 mass ratio mixing, is ultrasonically treated, obtains the second of suspension
It is swollen object；
S13：It is 1 to be swollen object and the second swelling object according to mass ratio by above-mentioned first:1~1.5 is mixed, and room temperature swelling 4~
Then 8h sequentially adds initiator, polymerized monomer, crosslinking agent and mentioned emulsifier, initiator, polymerized monomer, crosslinking agent and breast
The amount ratio of agent is 0.1:5~10:10~20:5~10, continue 6~12h of stirring；
S14：Above-mentioned solution is transferred to 80~120 DEG C of oil bath pans, it is 2~10 to sequentially add mass ratio:0.03~0.15:5
~25 stabilizers, coloring agent and ultra-pure water react 6~18h, after finally using the washing of 10~15vol% ethyl alcohol and gravity screening
Obtain the carboxylated micro-spheres of size uniformity.
3. a kind of preparation method of multi-fluorescence label polystyrene microsphere according to claim 2, it is characterised in that：Institute
It is lauryl sodium sulfate to state the emulsifier in step S11.
4. a kind of preparation method of multi-fluorescence label polystyrene microsphere according to claim 2, it is characterised in that：Institute
It is dibenzoyl peroxide to state initiator in step S13, and polymerized monomer is styrene, crosslinking agent be divinylbenzene or toluene or
Acrylic acid.
5. a kind of preparation method of multi-fluorescence label polystyrene microsphere according to claim 2, it is characterised in that：Institute
It is polyvinylpyrrolidone to state stabilizer in step S14, and coloring agent is methylene blue.
6. a kind of preparation method of multi-fluorescence label polystyrene microsphere according to claim 1, which is characterized in that step
The specific preparation process of quantum dot fluorescence microballoon is as follows in rapid S2：
S21：The carboxylic polystyrene microsphere is added in dispersant, is added a concentration of 10-2~10-8Mmol/L quantum dots
The mass ratio of solution, carboxylic polystyrene microsphere and quantum dot in solution is 1:10-3~10-9, it is ultrasonically treated and carries out solid-liquid point
From being then dried in vacuo solvent flashing；
S22：The solid volume ratio that S21 is obtained is 95:5 ethyl alcohol washs 2~3 times with hexamethylene mixed liquor, stores to super
In pure water, quantum dot fluorescence microballoon is obtained.
7. a kind of preparation method of multi-fluorescence label polystyrene microsphere according to claim 6, it is characterised in that：Institute
The dispersant of step S21 is stated as chloroform and isopropyl alcohol mixture, the volume ratio of chloroform and isopropanol is 95:5.
8. a kind of preparation method of multi-fluorescence label polystyrene microsphere according to claim 6, which is characterized in that step
The specific preparation process that quantum dot fluorescence microballoon and phycoerythrin are coupled in rapid S3 is as follows：
S31：It takes the quantum dot fluorescence microballoon in S22 to be dispersed in buffer solution and be added coupling agent, stirs 15~20min, obtain
Reaction solution, the mass ratio of quantum light fluorescent microsphere, buffer solution and coupling agent is 1 in reaction solution:500~600:0.15~0.3；
S32：Phycoerythrin is added in the reaction solution of S31, the quantum fluorescent microsphere in the phycoerythrin and reaction solution of addition
Mass ratio is 0.1~10:1,30~60 DEG C of rotation 2~4h of concussion reaction, are then washed with above-mentioned buffer solution.
9. a kind of preparation method of multi-fluorescence label polystyrene microsphere according to claim 8, it is characterised in that：Institute
It is 1 that state coupling agent, which be amount ratio,:2~5 1- ethyls-(3- dimethylaminopropyls) phosphinylidyne diimmonium salt hydrochlorate and N- hydroxyl ambers
Amber acid imide mixture.
10. a kind of preparation method of multi-fluorescence label polystyrene microsphere according to claim 8, it is characterised in that：
The buffer solution is 0.01mol/L PBS buffer solutions.
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| CN110305241A (en) * | 2019-05-13 | 2019-10-08 | 长春国科医工科技发展有限公司 | A kind of quantum dot fluorescence coding microball and preparation method thereof based on fluorescence resonance energy transfer analysis |
| CN112342014A (en) * | 2020-10-28 | 2021-02-09 | 安徽为臻生物工程技术有限公司 | Preparation method of monodisperse polymer fluorescent microspheres |
| WO2021109059A1 (en) * | 2019-12-05 | 2021-06-10 | 复旦大学 | Long-afterglow luminescent styrene polymer microsphere, preparation method therefor and application thereof |
| CN114163584A (en) * | 2021-12-30 | 2022-03-11 | 广东粤港澳大湾区国家纳米科技创新研究院 | Quantum dot fluorescent coding microsphere and preparation method thereof |
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| CN110305241A (en) * | 2019-05-13 | 2019-10-08 | 长春国科医工科技发展有限公司 | A kind of quantum dot fluorescence coding microball and preparation method thereof based on fluorescence resonance energy transfer analysis |
| WO2021109059A1 (en) * | 2019-12-05 | 2021-06-10 | 复旦大学 | Long-afterglow luminescent styrene polymer microsphere, preparation method therefor and application thereof |
| CN112342014A (en) * | 2020-10-28 | 2021-02-09 | 安徽为臻生物工程技术有限公司 | Preparation method of monodisperse polymer fluorescent microspheres |
| CN114163584A (en) * | 2021-12-30 | 2022-03-11 | 广东粤港澳大湾区国家纳米科技创新研究院 | Quantum dot fluorescent coding microsphere and preparation method thereof |
| CN114163584B (en) * | 2021-12-30 | 2024-01-19 | 朱小波 | Quantum dot fluorescent coding microsphere and preparation method thereof |
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