CN108341891A - A kind of extracting method of hericium erinaceum polysaccharide - Google Patents
A kind of extracting method of hericium erinaceum polysaccharide Download PDFInfo
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- CN108341891A CN108341891A CN201810437795.0A CN201810437795A CN108341891A CN 108341891 A CN108341891 A CN 108341891A CN 201810437795 A CN201810437795 A CN 201810437795A CN 108341891 A CN108341891 A CN 108341891A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21B—FIBROUS RAW MATERIALS OR THEIR MECHANICAL TREATMENT
- D21B1/00—Fibrous raw materials or their mechanical treatment
- D21B1/04—Fibrous raw materials or their mechanical treatment by dividing raw materials into small particles, e.g. fibres
- D21B1/12—Fibrous raw materials or their mechanical treatment by dividing raw materials into small particles, e.g. fibres by wet methods, by the use of steam
- D21B1/30—Defibrating by other means
- D21B1/36—Explosive disintegration by sudden pressure reduction
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
Abstract
The invention discloses a kind of extracting methods of hericium erinaceum polysaccharide, and dry Hericium erinaceus is smashed it through 80 mesh sieve through biomass pulverizer, places into and completes explosion in steam blasting device, by solid-to-liquid ratio 1:30 ~ 50 DEG C are heated to after 10 ~ 20 addition water immersion 1.5h to be ultrasonically treated, adjust feed temperature, pH value, protease and cellulase, heat up enzyme deactivation work, centrifugation, resin decolorization, de- albumen, filtering, reduced pressure, vacuum dehydrating at lower temperature after enzymolysis, obtain the hericium erinaceum polysaccharide solid powder of high activity, high-purity, improve polysaccharide extract rate and production efficiency, and the space structure and its bioactivity of reduction of polysaccharide will not be destroyed, it is more suitable for industrialized production.
Description
Technical field
The present invention relates to technical field of edible fungi production more particularly to a kind of extracting methods of hericium erinaceum polysaccharide.
Background technology
Hericium erinaceus also known as hedgehog hydnum mushroom, cauliflower bacterium, hedgehog bacterium etc. are mostly gained the name with shape, are a kind of rare food, medicinal fungus.
With one of bear's paw, bird's nest, neat China of shark's fin traditional " four big famous dishes ".Hedgehog hydnum fine and tender taste, it is soft, it is pleasant to the palate, it is full of nutrition.
According to surveying and determination, per 100g dry products 26.3g containing protein, fatty 4.2g, carbohydrate 44.9g, crude fibre 6.4g.Containing a variety of amino
Acid, wherein the amino acid that 7 kinds of human bodies need.Also contain multivitamin and mineral matter element.Except this, good pharmacology is also had concurrently
Effect, to disease of digestive system, such as stomach trouble, gastric ulcer has fine curative effect.In recent years, the monkey produced as raw material using hericium mycelium
Mushroom piece, Hericium erinaceus electuary, Hericium erinaceus syrup wait drugs constantly to come out.
Hericium erinaceum polysaccharide is the effective active composition extracted from high-quality hericium erinaceus fruiting body, and the main component of Hericium erinaceus is
Polysaccharide has the function of aid digestion, relieving the five internal organs, to a variety of disease of digestive tracts such as chronic gastritis, duodenal ulcer have compared with
Good curative effect, can improve human immunological competence.Modern medicine and nutrition deepen continuously research, practical fungi Mushroom polysaccharide, medicine
It is very high with being worth.The polysaccharide extracted from hericium erinaceus fruiting body is main chain by β -1, and 3 glycosidic bonds, branch is β -1,6 glycosidic bonds
The glucan mixture of connection, molecular weight is between 8000 ~ 40000.A large amount of medicine and pharmaceutical research show the Hericium erinaceus
Polysaccharide, which has, improves the physiological functions such as immunity, antitumor, anti-aging, reducing blood lipid, to the canceration of digestive system, ulcer, antrum
The disease such as inflammation, chronic gastritis is effective in cure, and can improve the immunocompetence of human body.Therefore, drug and guarantor made of Hericium erinaceus polysaccharide
Strong product are favored by people, and development prospect is wide.
The extracting method of currently used hericium erinaceum polysaccharide has alkaline extraction, sour formulation, salt formulation to also have Hot water extraction.It is dilute
Acid, diluted alkaline method, reagent dosage is few, and instrument is conventional instrument, therefore operation is simple, but under the conditions of diluted acid, diluted alkaline, easily makes
The fracture of glycosidic bond occurs for polysaccharide, and par-tial polysaccharide occurs hydrolysis and the recovery rate of polysaccharide is made to reduce, and impurity content also compares
It is more, there is certain scope of application.Although the constant temperature refluxing extraction of pure physics has lower production cost, when due to operation
Between the long, drawbacks such as recovery rate is low, limit it and promote the use.The microwave radiation exaraction time is short, yield is high, but since microwave has
There is corner fuel factor, be easy to cause uneven heating, influences recovery rate；And it is used for thermal instability substance, apolar substance and height
When viscosity materials are extracted, extraction efficiency can be also influenced.Liu Zhen Lam patent of invention " process for refinement and purification of fungus polysaccharide ", which indicates, to be carried
Pure granulose method (200610012682.3), but for hericium erinaceum polysaccharide, itself existing makes the deeper phenol of color
Type compound is connect with sugar chain, it is more difficult to be removed, and there is also the interference of the large biological molecules such as cellulose, pectin, ultrafiltration fenestra is easy
The problems such as obstruction.Therefore, the hericium erinaceum polysaccharide that thus method obtains needs further to carry there is also product color, efficiency, purity etc.
High defect.
Based on above-mentioned analysis, the present invention provides a kind of, and, supersound process joint quick-fried by steam extracts hedgehog hydnum in a manner of digesting
Mushroom polysaccharide is broken caused by effectively having evaded acid-base pair glycosidic bond, ultrasound is combined together with the characteristics of steam blasting, effectively more
The deficiency that ultrasound and steam blasting are individually handled has been mended, and has concentrated the advantages of remaining two kinds of extracting methods, prominent be embodied in is shaken
It is dynamic to homogenize, each point effect in sample media can be made consistent, and the binding force of object and sample matrices can be reduced, accelerate object
Enter the process of solvent phase, it can be achieved that the largely extraction of hericium erinaceum polysaccharide from solid phase.
Invention content
In view of above-mentioned deficiency, the present invention provides a kind of, and, supersound process joint quick-fried by steam extracts hedgehog hydnum in a manner of digesting
Mushroom polysaccharide is broken caused by effectively having evaded acid-base pair glycosidic bond, while purity of polysaccharide is high, easy to operate, and it is more to be suitable for industry
The extraction of sugar.
The present invention is achieved by the following technical solutions：
（1）It crushes：Dry Hericium erinaceus is crushed through biomass pulverizer, 80 mesh sieve is crossed, obtains hedgehog hydnum mushroom powder；
（2）Steam blasting：It takes 100g hedgehog hydnum mushroom powders to be put into steam blasting device and completes explosion, obtain explosion hedgehog hydnum mushroom powder；
（3）It is ultrasonically treated：Water is added according to solid-to-liquid ratio in explosion hedgehog hydnum mushroom powder and impregnates 1.5h, and is aided with ultrasound at 30 ~ 50 DEG C
Processing, obtains material liquid mixture；
（4）Proteolysis：Will, it obtains material liquid mixture and is added in retort, control temperature, the pH value of retort, put into protease,
Persistently 1 ~ 2h of stirring carries out proteolysis；
（5）Fiber digests：Again control retort in feed liquid temperature and pH value, then put into cellulase, persistently stir 1 ~
2h carries out fiber enzymolysis, obtains enzymolysis liquid；
（6）Enzyme deactivation is lived：Feed liquid is warming up to 85 DEG C, keeps 15min, obtains enzyme deactivation work liquid；
（7）Decoloration：Enzyme deactivation is lived after liquid centrifugation, and recycling feed liquid is in bleacher, and circulating water is to 40 ~ 60 DEG C, input nonpolarity
Macroporous absorbent resin, decolourize 1h under stirring, obtains decoloration feed liquid；
（8）It is concentrated under reduced pressure：Low-temperature reduced-pressure is concentrated into the 1/3 of original volume after decoloration feed liquid filtering, obtains hericium erinaceum polysaccharide concentrate；
（9）It is dry：Hericium erinaceum polysaccharide concentrate obtains hericium erinaceum polysaccharide solid powder after vacuum dehydrating at lower temperature.
Further, the steam blasting pressure is 1.5MPa-2.0MPa, temperature is 100 DEG C -200 DEG C, the time is
2min-5min。
Further, the solid-liquid ratio 1 soaked:10~20.
Further, the ultrasonic power is 3000 ~ 4000W, and the processing time is 30min.
Further, the proteolysis temperature is 45 ~ 60 DEG C, and pH value 6.0 ~ 8.0, albumen enzyme dosage accounts for hedgehog hydnum mushroom powder weight
Measure ratio 0.5 ~ 1.5%.
Further, the fiber hydrolysis temperature is 45 ~ 60 DEG C, and pH value 3.0 ~ 6.0, cellulase dosage accounts for hedgehog hydnum mushroom powder
Weight ratio 1.0 ~ 2.0%.
Further, the nonpolar macroporous adsorption resin dosage accounts for centrifugation feed liquid weight ratio 5.0 ~ 10.0%.
Further, the low-temperature reduced-pressure thickening temperature is 50 ~ 80 DEG C.
Further, the vacuum dehydrating at lower temperature temperature is 60 ~ 90 DEG C.
The beneficial effects of the present invention are：
1, hericium erinaceum polysaccharide is extracted by the way of steam explosion, supersound process joint enzymolysis, has effectively evaded acid-base pair sugar
Fracture, the purity of polysaccharide higher of extraction caused by glycosidic bond.
2, ultrasound is combined together with the characteristics of steam blasting, effectively compensates for ultrasound and steam blasting is individually handled not
Enough, and the advantages of remaining two kinds of extracting methods, protrusion is concentrated to be embodied in vibration and homogenize, each point effect one in sample media can be made
It causes.
3, the present invention can reduce the binding force of object and sample matrices, and object is accelerated to enter the mistake of solvent phase from solid phase
Journey is, it can be achieved that the largely extraction of hericium erinaceum polysaccharide.
Specific implementation mode
Embodiment 1
A kind of extracting method of hericium erinaceum polysaccharide
（1）It crushes：Dry Hericium erinaceus is crushed through biomass pulverizer, 80 mesh sieve is crossed, obtains hedgehog hydnum mushroom powder；
（2）Steam blasting：100g hedgehog hydnum mushroom powders are put into steam blasting device and complete explosion, burst pressure 1.5MPa, temperature
For 100 DEG C, maintain explosion 5min, then the pressure in reactor is reduced into 1 standard atmospheric pressure, after completing explosion, obtains explosion monkey
Head mushroom powder；
（3）It impregnates：Explosion hedgehog hydnum mushroom powder is added in retort, according to solid-to-liquid ratio 1:15, which are added water, impregnates 1.5h, and it is mixed to obtain feed liquid
Close object；
（4）It is ultrasonically treated：Material liquid mixture carries out 30min supersound process, ultrasonic power 3500W at 40 DEG C；
（5）Proteolysis：55 DEG C of temperature, the pH value 6.0 for controlling feed liquid in retort put into hedgehog hydnum mushroom powder weight into retort
Protease than 0.5% digests 2h under stirring；
（6）Fiber digests：55 DEG C of temperature, the pH value 3.0 for controlling feed liquid in retort put into hedgehog hydnum mushroom powder weight into retort
Cellulase than 1.0% digests 2h under stirring；
（7）Enzyme deactivation is lived：Feed liquid is warming up to 85 DEG C, keeps 15min, obtains enzymolysis liquid；
（8）Decoloration：After enzymolysis liquid centrifugation, recycling feed liquid is in bleacher, and circulating water is to 50 DEG C, input centrifugation feed liquid 7.5%
Nonpolar macroporous adsorption resin（Resin provides producer：ROHM AND HAAS, resin model：AMBERLITE XAD16 macroporous absorption trees
Fat）, decolourize 1h under stirring, obtains decoloration feed liquid；
（9）It is concentrated under reduced pressure：Feed liquid of decolourizing filtering, 80 DEG C of downlink low-temperature reduced-pressures are concentrated into the 1/3 of original volume, obtain hericium erinaceum polysaccharide
Concentrate；
（10）It is dry：Hericium erinaceum polysaccharide concentrate obtains hericium erinaceum polysaccharide solid powder after vacuum dehydrating at lower temperature.
Embodiment 2
A kind of extracting method of hericium erinaceum polysaccharide
（1）It crushes：Dry Hericium erinaceus is crushed through biomass pulverizer, 80 mesh sieve is crossed, obtains hedgehog hydnum mushroom powder；
（2）Steam blasting：100g hedgehog hydnum mushroom powders are put into steam blasting device and complete explosion, steam blasting pressure 1.7MPa,
Temperature is 150 DEG C, maintains explosion 2.5min, obtains explosion hedgehog hydnum mushroom powder；
（3）It impregnates：Explosion hedgehog hydnum mushroom powder is added in retort, according to solid-to-liquid ratio 1:10, which are added water, impregnates 1.5h, and it is mixed to obtain feed liquid
Close object；
（4）It is ultrasonically treated：Material liquid mixture carries out 30min supersound process, ultrasonic power 3000W at 50 DEG C；
（5）Proteolysis：55 DEG C of temperature, the pH value 7.0 for controlling feed liquid in retort put into hedgehog hydnum mushroom powder weight into retort
Protease than 1.0% digests 1.5h under stirring；
（6）Fiber digests：55 DEG C of temperature, the pH value 4.5 for controlling feed liquid in retort put into hedgehog hydnum mushroom powder weight into retort
Cellulase than 2.0% digests 1.5h under stirring；
（7）Enzyme deactivation is lived：Feed liquid is warming up to 85 DEG C, keeps 15min, obtains enzymolysis liquid；
（8）Decoloration：After enzymolysis liquid centrifugation, recycling feed liquid is in bleacher, and circulating water is to 50 DEG C, input centrifugation feed liquid 5.0%
Nonpolar macroporous adsorption resin, decolourize 1h under stirring, obtains decoloration feed liquid；
（9）It is concentrated under reduced pressure：Feed liquid of decolourizing filtering, 80 DEG C of downlink low-temperature reduced-pressures are concentrated into the 1/3 of original volume, obtain hericium erinaceum polysaccharide
Concentrate；
（10）It is dry：Hericium erinaceum polysaccharide concentrate obtains hericium erinaceum polysaccharide solid powder after vacuum dehydrating at lower temperature.
Embodiment 3
A kind of extracting method of hericium erinaceum polysaccharide
（1）It crushes：Dry Hericium erinaceus is crushed through biomass pulverizer, 80 mesh sieve is crossed, obtains hedgehog hydnum mushroom powder；
（2）Steam blasting：100g hedgehog hydnum mushroom powders are put into steam blasting device and complete explosion, steam blasting pressure 2.0MPa,
Temperature is 200 DEG C, maintains explosion 2min, obtains explosion hedgehog hydnum mushroom powder；
（3）It impregnates：Explosion hedgehog hydnum mushroom powder is added in retort, according to solid-to-liquid ratio 1:20, which are added water, impregnates 1.5h, and it is mixed to obtain feed liquid
Close object；
（4）It is ultrasonically treated：Material liquid mixture carries out 30min supersound process, ultrasonic power 4000W at 50 DEG C；
（5）Proteolysis：52 DEG C of temperature, the pH value 7.0 for controlling feed liquid in retort put into hedgehog hydnum mushroom powder weight into retort
Protease than 1.0% digests 2h under stirring；
（6）Fiber digests：52 DEG C of temperature, the pH value 4.5 for controlling feed liquid in retort put into hedgehog hydnum mushroom powder weight into retort
Cellulase than 2.0% digests 2h under stirring；
（7）Enzyme deactivation is lived：Feed liquid is warming up to 85 DEG C, keeps 15min, obtains enzymolysis liquid；
（8）Decoloration：After enzymolysis liquid centrifugation, recycling feed liquid is in bleacher, and circulating water is to 55 DEG C, input centrifugation feed liquid
10.0% nonpolar macroporous adsorption resin, decolourize 1h under stirring, obtains decoloration feed liquid；
（9）It is concentrated under reduced pressure：Feed liquid of decolourizing filtering, 80 DEG C of downlink low-temperature reduced-pressures are concentrated into the 1/3 of original volume, obtain hericium erinaceum polysaccharide
Concentrate；
（10）It is dry：Hericium erinaceum polysaccharide concentrate obtains hericium erinaceum polysaccharide solid powder after vacuum dehydrating at lower temperature.
Comparative example 1
Extraction step is same as Example 1, only cancels Steam explosion treatment.
Comparative example 2
Extraction step is same as Example 1, only cancels being ultrasonically treated.
Comparative example 3
Extraction step is same as Example 1, only cancels Steam explosion treatment and is ultrasonically treated.
Test example
The detection of polyoses content
It chooses hericium erinaceum polysaccharide 10mg obtained in embodiment 1 ~ 3, test example 1 ~ 3 to be placed in 100ml beakers, 80ml is added to steam again
Boiling boils 1 hour postcooling to room temperature, then adds redistilled water constant volume to 100ml.It measures 1.0ml to move into cuvette, adds 5.0%
Redistilled phenol 2.0ml, then enriching sulfuric acid 5.0ml, 20min is placed at room temperature for after shaking up, and absorbance is measured at wavelength 490nm；Again
Heavy metal analysis is carried out to the hericium erinaceum polysaccharide obtained by each group to detect with sanitary index, the results are shown in Table 1.
The different group hericium erinaceum polysaccharide assays of table 1
。
As shown in Table 1, for the hericium erinaceum polysaccharide yield that prepared by embodiment 1 ~ 3 80% or more, comparative example 1 compares embodiment 1
Cancel Steam explosion treatment, hericium erinaceum polysaccharide yield is 71% significantly lower than embodiment 1；Comparative example 2 is cancelled super compared to embodiment 1
Sonication, hericium erinaceum polysaccharide yield are significantly lower than embodiment 1 but slightly above comparative example 1 for 73%；Comparative example 3 takes compared to embodiment 1
Disappear Steam explosion treatment and supersound process, and hericium erinaceum polysaccharide yield is 65% significantly lower than comparative example 1.Illustrate only quick-fried in steam
Under collaboration that is broken and being ultrasonically treated is handled, hericium erinaceum polysaccharide yield could be improved.
The above, only presently preferred embodiments of the present invention, not to the present invention in any form with substantial limitation,
It should be pointed out that for those skilled in the art, under the premise of not departing from the method for the present invention, can also make
Several improvement and supplement, these are improved and supplement also should be regarded as protection scope of the present invention.All those skilled in the art,
Without departing from the spirit and scope of the present invention, when made using disclosed above technology contents it is a little more
Dynamic, modification and the equivalent variations developed, are the equivalent embodiment of the present invention；Meanwhile all substantial technologicals pair according to the present invention
The variation, modification and evolution of any equivalent variations made by above-described embodiment, still fall within the range of technical scheme of the present invention
It is interior.
Claims (10)
1. a kind of extracting method of hericium erinaceum polysaccharide, which is characterized in that this approach includes the following steps：
（1）It crushes：Dry Hericium erinaceus is crushed through biomass pulverizer, 80 mesh sieve is crossed, obtains hedgehog hydnum mushroom powder；
（2）Steam blasting：It takes 100g hedgehog hydnum mushroom powders to be put into steam blasting device and completes explosion, obtain explosion hedgehog hydnum mushroom powder；
（3）It is ultrasonically treated：Water is added according to solid-to-liquid ratio in explosion hedgehog hydnum mushroom powder and impregnates 1.5h, and is aided with ultrasound at 30 ~ 50 DEG C
Processing, obtains material liquid mixture；
（4）Proteolysis：Material liquid mixture is added in retort, temperature, the pH value of retort are controlled, protease is put into, holds
1 ~ 2h of continuous stirring carries out proteolysis；
（5）Fiber digests：Again control retort in feed liquid temperature and pH value, then put into cellulase, persistently stir 1 ~
2h carries out fiber enzymolysis, obtains enzymolysis liquid；
（6）Enzyme deactivation is lived：Feed liquid is warming up to 85 DEG C, keeps 15min, obtains enzyme deactivation work liquid；
（7）Decoloration：Enzyme deactivation is lived after liquid centrifugation, and recycling feed liquid is in bleacher, and circulating water is to 40 ~ 60 DEG C, input nonpolarity
Macroporous absorbent resin, decolourize 1h under stirring, obtains decoloration feed liquid；
（8）It is concentrated under reduced pressure：Low-temperature reduced-pressure is concentrated into the 1/3 of original volume after decoloration feed liquid filtering, obtains hericium erinaceum polysaccharide concentrate；
（9）It is dry：Hericium erinaceum polysaccharide concentrate obtains hericium erinaceum polysaccharide solid powder after vacuum dehydrating at lower temperature.
2. extracting method according to claim 1, which is characterized in that the steam blasting pressure be 1.5MPa-2.0MPa,
Temperature is 100 DEG C -200 DEG C, time 2min-5min.
3. extracting method according to claim 1, which is characterized in that the solid-liquid ratio 1 soaked:10~20.
4. extracting method according to claim 1, which is characterized in that the ultrasonic power is 3000 ~ 4000W, the place
The reason time is 30min.
5. extracting method according to claim 1, which is characterized in that the proteolysis temperature is 45 ~ 60 DEG C, pH value
6.0 ~ 8.0, albumen enzyme dosage accounts for hedgehog hydnum mushroom powder weight ratio 0.5 ~ 1.5%.
6. extracting method according to claim 1, which is characterized in that the fiber hydrolysis temperature is 45 ~ 60 DEG C, pH value
3.0 ~ 6.0, cellulase dosage accounts for hedgehog hydnum mushroom powder weight ratio 1.0 ~ 2.0%.
7. extracting method according to claim 1, which is characterized in that the nonpolar macroporous adsorption resin dosage accounts for centrifugation
Feed liquid weight ratio 5.0 ~ 10.0%.
8. extracting method according to claim 1, which is characterized in that the low-temperature reduced-pressure thickening temperature is 50 ~ 80 DEG C.
9. extracting method according to claim 1, which is characterized in that the vacuum dehydrating at lower temperature temperature is 60 ~ 90 DEG C.
10. a kind of according to hericium erinaceum polysaccharide made from any means described in claim 1 ~ 9.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336991A (en) * | 2018-11-05 | 2019-02-15 | 福建拓天生物科技有限公司 | A kind of extracting method of hericium erinaceum polysaccharide |
| CN110894518A (en) * | 2019-11-05 | 2020-03-20 | 上海加新生物科技有限公司 | Preparation method of hericium erinaceus small peptide |
| CN110903408A (en) * | 2019-11-01 | 2020-03-24 | 福州福德恩生物科技有限公司 | Preparation method of hericium erinaceus polysaccharide protein extract |
| CN111333746A (en) * | 2020-05-09 | 2020-06-26 | 福州康来生物科技有限公司 | Extraction method of hericium erinaceus polysaccharide |
| CN111466571A (en) * | 2020-04-14 | 2020-07-31 | 安徽斯普瑞生物科技有限公司 | Preparation method of edible and medicinal hericium erinaceus dry powder |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101358224A (en) * | 2008-09-04 | 2009-02-04 | 浙江益圣菌物发展有限公司 | Extraction method of hericium erinaceus polysaccharide |
| CN104480162A (en) * | 2014-11-28 | 2015-04-01 | 哈尔滨墨医生物技术有限公司 | Method for efficiently extracting hericium erinaceus polysaccharides |
| CN105384836A (en) * | 2015-12-16 | 2016-03-09 | 黑龙江众生生物工程有限公司 | Method for improving extraction leaching rate of medicinal and edible fungus beta-glucan |
| CN106478838A (en) * | 2016-12-02 | 2017-03-08 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of extracting method of high-purity edible fungi polysaccharide |
-
2018
- 2018-05-09 CN CN201810437795.0A patent/CN108341891A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101358224A (en) * | 2008-09-04 | 2009-02-04 | 浙江益圣菌物发展有限公司 | Extraction method of hericium erinaceus polysaccharide |
| CN104480162A (en) * | 2014-11-28 | 2015-04-01 | 哈尔滨墨医生物技术有限公司 | Method for efficiently extracting hericium erinaceus polysaccharides |
| CN105384836A (en) * | 2015-12-16 | 2016-03-09 | 黑龙江众生生物工程有限公司 | Method for improving extraction leaching rate of medicinal and edible fungus beta-glucan |
| CN106478838A (en) * | 2016-12-02 | 2017-03-08 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of extracting method of high-purity edible fungi polysaccharide |
Non-Patent Citations (5)
| Title |
|---|
| 何东平等: "《木本油料加工技术》", 31 October 2016, 中国轻工业出版社 * |
| 张东方等: "《中药现代分离技术》", 31 May 2006, 辽宁大学出版社 * |
| 张立主编: "《猴头菇与胃健康》", 30 November 2014, 中国医药科技出版社 * |
| 张素斌等: ""猴头菇多糖提取方法的比较"", 《食品与发酵工业》 * |
| 赵余庆主编: "《中药及天然产物提取制备关键技术》", 31 January 2012, 中国医药科技出版社 * |
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| CN110903408A (en) * | 2019-11-01 | 2020-03-24 | 福州福德恩生物科技有限公司 | Preparation method of hericium erinaceus polysaccharide protein extract |
| CN110894518A (en) * | 2019-11-05 | 2020-03-20 | 上海加新生物科技有限公司 | Preparation method of hericium erinaceus small peptide |
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