CN108341888A - A kind of preparation method of argyi leaf polysaccharide extract - Google Patents
A kind of preparation method of argyi leaf polysaccharide extract Download PDFInfo
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- CN108341888A CN108341888A CN201810218970.7A CN201810218970A CN108341888A CN 108341888 A CN108341888 A CN 108341888A CN 201810218970 A CN201810218970 A CN 201810218970A CN 108341888 A CN108341888 A CN 108341888A
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- filtrate
- argyi
- filter residue
- leaf polysaccharide
- folium artemisiae
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Biochemistry (AREA)
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Abstract
The invention discloses a kind of preparation methods of argyi leaf polysaccharide extract, include the following steps：(1) folium artemisiae argyi is crushed into processing, is 1 according to solid-liquid ratio:Pure water is added in the ratio of 68 75g/mL, extracts 150 180min under the conditions of pH is 80 85 DEG C of 7.6 8, temperature, filter to get filtrate a and folium artemisiae argyi filter residue；(2) folium artemisiae argyi filter residue is taken, petroleum ether 180 220min of reflux, centrifugation, filter residue is after 40 DEG C of drying, 220 250min of alcohol reflux, centrifugation, gained filter residue is used to add 40 times of deionized waters after 40 DEG C of drying again, 10h is extracted in 80 85 DEG C of water-baths, is repeated 4 times, filtrate b is collected by centrifugation to obtain；(3) filtrate a and filtrate b is mixed, is concentrated under reduced pressure, dry argyi leaf polysaccharide extract.The argyi leaf polysaccharide recovery rate of the present invention is higher and purer.
Description
Technical field
The present invention relates to technical field of plant extraction, and in particular to a kind of preparation method of argyi leaf polysaccharide extract.
Background technology
Polysaccharide is the substance being widely present in organism, and the effect in organism functions not only as energy resource or structure
Material, it is often more important that take part in the various activities of cell in biological phenomena, there is diversified biological function.Largely grind
Study carefully and show polysaccharide in addition to immunological regulation, anti-tumor biological effect, also anticomplement, anti-inflammatory, hypoglycemic, antiviral
The effects that activity, anti-aging, anticoagulation, anti-hepatic fibrosis, and it is small to Body adverse reaction.Some polysaccharide have become treatment disease
The drug and health food of disease have higher Development volue.Currently, having nearly hundred kinds of plant glycocalix extraction identifications, because of it
Source is wide and small toxicity, is widely used in field of medicaments.With being understood in depth to active polysaccharide and people are to various natural
Drug, wholefood push away haunt and plague, and polysaccharide is small with its adverse reaction, and is having for the functional health products such as nutrition, medical assistance again
Ingredient is imitated, causes to pay attention to extensively.
Folium artemisiae argyi is the leaf of composite family herbaceos perennial Chinese mugwort, and national most area is produced.A large amount of pharmacological research proves
Folium artemisiae argyi has anti-bacteria and anti-virus, antiasthmatic effect eliminating the phlegm, hemostasis and anticoagulation, immunological regulation, antiallergy, calmness, protect liver cholagogic etc.
Effect.Folium artemisiae argyi also contains polysaccharide, tannin, flavones, sterol, trace element and other organic principles etc. other than containing volatile oil.
Invention content
In view of this, the object of the present invention is to provide a kind of preparation methods of argyi leaf polysaccharide extract.
The technical solution adopted by the present invention is that：
A kind of preparation method of argyi leaf polysaccharide extract, includes the following steps：
(1) folium artemisiae argyi is crushed into processing, is 1 according to solid-liquid ratio:Pure water is added in the ratio of 68-75g/mL, is in pH
150-180min is extracted under the conditions of 7.6-8,80-85 DEG C of temperature, filter to get filtrate a and folium artemisiae argyi filter residue；
(2) folium artemisiae argyi filter residue is taken, petroleum ether reflux 180-220min, centrifugation, filter residue is after 40 DEG C of drying, then uses alcohol reflux
220-250min, centrifugation, gained filter residue add 40 times of deionized waters, 80-85 DEG C of water-bath to extract 10h, repeat 4 after 40 DEG C of drying
It is secondary, filtrate b is collected by centrifugation to obtain；
(3) filtrate a and filtrate b is mixed, is concentrated under reduced pressure, dry argyi leaf polysaccharide extract.
Preferably, the solid-liquid ratio described in step (1) is 1:70g/mL, extraction time 160min.
Preferably, the ethyl alcohol that the ethyl alcohol described in step (2) is 95%.
Preferably, argyi leaf polysaccharide extract obtained is washed 3-4 times with absolute ethyl alcohol, acetone respectively successively, is then frozen
It does to constant weight, is then preserved.
Preferably, the present invention provides a kind of argyi leaf polysaccharide extracts, and method of the present invention is used to prepare.
It is an advantage of the invention that：The argyi leaf polysaccharide recovery rate of the present invention is higher and purer.
Specific implementation mode
For a further understanding of the present invention, the preferred embodiment of the invention is described with reference to embodiment, still
It should be appreciated that these descriptions are only the feature and advantage further illustrated the present invention, rather than to the claims in the present invention
Limitation.
Embodiment 1
A kind of preparation method of argyi leaf polysaccharide extract, includes the following steps：
(1) folium artemisiae argyi is crushed into processing, is 1 according to solid-liquid ratio:Pure water is added in the ratio of 68g/mL, pH be 7.6,
150min is extracted under the conditions of 80 DEG C of temperature, filter to get filtrate a and folium artemisiae argyi filter residue；
(2) folium artemisiae argyi filter residue is taken, petroleum ether reflux 180min, centrifugation, filter residue is after 40 DEG C of drying, then uses alcohol reflux
220min, centrifugation, gained filter residue add 40 times of deionized waters, 80 DEG C of water-baths to extract 10h, be repeated 4 times, centrifuge after 40 DEG C of drying
Collect to obtain filtrate b；
(3) filtrate a and filtrate b is mixed, is concentrated under reduced pressure, dry argyi leaf polysaccharide extract；
(4) argyi leaf polysaccharide extract obtained is washed 3 times with absolute ethyl alcohol, acetone respectively successively, is then lyophilized to perseverance
Weight, is then preserved.
Embodiment 2
A kind of preparation method of argyi leaf polysaccharide extract, includes the following steps：
(1) folium artemisiae argyi is crushed into processing, is 1 according to solid-liquid ratio:Pure water is added in the ratio of 71g/mL, pH be 7.8,
165min is extracted under the conditions of 83 DEG C of temperature, filter to get filtrate a and folium artemisiae argyi filter residue；
(2) folium artemisiae argyi filter residue is taken, petroleum ether reflux 200min, centrifugation, filter residue is after 40 DEG C of drying, then uses alcohol reflux
235min, centrifugation, gained filter residue add 40 times of deionized waters, 83 DEG C of water-baths to extract 10h, be repeated 4 times, centrifuge after 40 DEG C of drying
Collect to obtain filtrate b；
(3) filtrate a and filtrate b is mixed, is concentrated under reduced pressure, dry argyi leaf polysaccharide extract；
(4) argyi leaf polysaccharide extract obtained is washed 3 times with absolute ethyl alcohol, acetone respectively successively, is then lyophilized to perseverance
Weight, is then preserved.
Embodiment 3
A kind of preparation method of argyi leaf polysaccharide extract, includes the following steps：
(1) folium artemisiae argyi is crushed into processing, is 1 according to solid-liquid ratio:Pure water is added in the ratio of 75g/mL, pH be 8, temperature
180min is extracted under the conditions of 85 DEG C of degree, filter to get filtrate a and folium artemisiae argyi filter residue；
(2) folium artemisiae argyi filter residue is taken, petroleum ether reflux 220min, centrifugation, filter residue is after 40 DEG C of drying, then uses alcohol reflux
250min, centrifugation, gained filter residue add 40 times of deionized waters, 85 DEG C of water-baths to extract 10h, be repeated 4 times, centrifuge after 40 DEG C of drying
Collect to obtain filtrate b；
(3) filtrate a and filtrate b is mixed, is concentrated under reduced pressure, dry argyi leaf polysaccharide extract；
(4) argyi leaf polysaccharide extract obtained is washed 4 times with absolute ethyl alcohol, acetone respectively successively, is then lyophilized to perseverance
Weight, is then preserved.
Claims (5)
1. a kind of preparation method of argyi leaf polysaccharide extract, it is characterised in that include the following steps：
(1) folium artemisiae argyi is crushed into processing, is 1 according to solid-liquid ratio:Pure water is added in the ratio of 68-75g/mL, is 7.6- in pH
8,150-180min is extracted under the conditions of 80-85 DEG C of temperature, filter to get filtrate a and folium artemisiae argyi filter residue；
(2) folium artemisiae argyi filter residue is taken, petroleum ether flows back 180-220min, centrifugation, and filter residue is after 40 DEG C of drying, then with alcohol reflux 220-
250min, centrifugation, gained filter residue add 40 times of deionized waters, 80-85 DEG C of water-bath to extract 10h, be repeated 4 times after 40 DEG C of drying, from
The heart collects to obtain filtrate b；
(3) filtrate a and filtrate b is mixed, is concentrated under reduced pressure, dry argyi leaf polysaccharide extract.
2. preparation method according to claim 1, it is characterised in that the solid-liquid ratio described in step (1) is 1:70g/mL is carried
It is 160min to take the time.
3. preparation method according to claim 1, it is characterised in that the ethyl alcohol that the ethyl alcohol described in step (2) is 95%.
4. preparation method according to claim 1, it is characterised in that by argyi leaf polysaccharide extract obtained successively with anhydrous
Ethyl alcohol, acetone wash 3-4 times respectively, are then lyophilized to constant weight, are then preserved.
5. a kind of argyi leaf polysaccharide extract, it is characterised in that prepared using claim 1-4 any one of them methods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810218970.7A CN108341888A (en) | 2018-03-16 | 2018-03-16 | A kind of preparation method of argyi leaf polysaccharide extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810218970.7A CN108341888A (en) | 2018-03-16 | 2018-03-16 | A kind of preparation method of argyi leaf polysaccharide extract |
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| Publication Number | Publication Date |
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| CN108341888A true CN108341888A (en) | 2018-07-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810218970.7A Pending CN108341888A (en) | 2018-03-16 | 2018-03-16 | A kind of preparation method of argyi leaf polysaccharide extract |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929047A (en) * | 2019-02-26 | 2019-06-25 | 杭州师范大学 | A kind of extracting method of argyi leaf polysaccharide |
| CN111636192A (en) * | 2020-06-02 | 2020-09-08 | 广东洪兴实业股份有限公司 | Wormwood dyeing and finishing agent and application method thereof in preparation of antibacterial fibers |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1962698A (en) * | 2006-11-17 | 2007-05-16 | 华东理工大学 | Use of argyi leaf polysaccharide extract |
| CN102180985A (en) * | 2011-03-25 | 2011-09-14 | 安徽农业大学 | Method for extracting and purifying mugwort polysaccharides |
-
2018
- 2018-03-16 CN CN201810218970.7A patent/CN108341888A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1962698A (en) * | 2006-11-17 | 2007-05-16 | 华东理工大学 | Use of argyi leaf polysaccharide extract |
| CN102180985A (en) * | 2011-03-25 | 2011-09-14 | 安徽农业大学 | Method for extracting and purifying mugwort polysaccharides |
Non-Patent Citations (1)
| Title |
|---|
| 刘军海等: "响应面分析法优化艾叶多糖提取工艺研究", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929047A (en) * | 2019-02-26 | 2019-06-25 | 杭州师范大学 | A kind of extracting method of argyi leaf polysaccharide |
| CN111636192A (en) * | 2020-06-02 | 2020-09-08 | 广东洪兴实业股份有限公司 | Wormwood dyeing and finishing agent and application method thereof in preparation of antibacterial fibers |
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