CN108340571A - A kind of more biological 3D printing forming methods of nozzle coordination - Google Patents
A kind of more biological 3D printing forming methods of nozzle coordination Download PDFInfo
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- CN108340571A CN108340571A CN201810128395.1A CN201810128395A CN108340571A CN 108340571 A CN108340571 A CN 108340571A CN 201810128395 A CN201810128395 A CN 201810128395A CN 108340571 A CN108340571 A CN 108340571A
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- printing
- slurry
- sodium alginate
- nozzles
- forming method
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- 238000010146 3D printing Methods 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 45
- 239000002002 slurry Substances 0.000 claims abstract description 91
- 238000007639 printing Methods 0.000 claims abstract description 87
- 239000000463 material Substances 0.000 claims abstract description 58
- 238000000465 moulding Methods 0.000 claims abstract description 21
- 239000000661 sodium alginate Substances 0.000 claims description 47
- 229940005550 sodium alginate Drugs 0.000 claims description 47
- 235000010413 sodium alginate Nutrition 0.000 claims description 34
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 16
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 13
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 239000012153 distilled water Substances 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- 239000007943 implant Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 239000011159 matrix material Substances 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims description 4
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims description 4
- 238000004090 dissolution Methods 0.000 claims description 4
- 210000002744 extracellular matrix Anatomy 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 239000003755 preservative agent Substances 0.000 claims description 4
- 230000002335 preservative effect Effects 0.000 claims description 4
- 239000011149 active material Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 2
- 239000003102 growth factor Substances 0.000 abstract description 9
- 238000009826 distribution Methods 0.000 abstract description 6
- 239000004615 ingredient Substances 0.000 abstract description 5
- 238000007493 shaping process Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 13
- 230000000694 effects Effects 0.000 description 9
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- -1 cell Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000007641 inkjet printing Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000002805 bone matrix Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000007613 slurry method Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004506 ultrasonic cleaning Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/10—Processes of additive manufacturing
- B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
- B29C64/112—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using individual droplets, e.g. from jetting heads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Manufacturing & Machinery (AREA)
- Physics & Mathematics (AREA)
- Mechanical Engineering (AREA)
- Optics & Photonics (AREA)
Abstract
The present invention relates to a kind of more nozzles to coordinate biological 3D printing forming method, including：It is configured to multigroup slurry of printing three-dimensional rack, all slurries are divided into first kind slurry and the second class slurry according to viscosity height, and first kind slurry is high viscosity fluidised form moulding material, and the second class slurry is low viscosity fluidised form moulding material；3D printing model is established, the printing path collection of three-dimensional rack is obtained；One or more slurries of first kind slurry are formed into skeleton by the electronic extruded type nozzle of corresponding number along printing path collection jet printing；One or more slurries of second class slurry are ejected into along printing path collection on skeleton by the pneumatic injecting type nozzle of corresponding number, three-dimensional rack is obtained after the completion of printing.The printing shaping method realizes the precise Printing of the continuous printing and middle low viscosity cell, growth factor bio-ink of high viscosity timbering material, meets the gradient distribution of the needs of model labyrinth and bore diameter gradient variation and ingredient spatially.
Description
Technical field
The present invention relates to 3D printing technique field, more particularly to a kind of more nozzles coordinate biological 3D printing forming method.
Background technology
It is main in bioengineered tissue in recent years that biomaterial is still built into tool by various 3D printing techniques
There is the porous support of specific structure, is implanted into again after being then implanted directly into lesion or the upper biotic factor of plantation, cell to study
Large segmental bone defect Regeneration and Repair.Although the result shows that the molding holder of these methods has certain Bone Defect Repari effect, due to can
Can pyroprocess and printing type unification, only realize the bionical of structure, weaken seed cell and biotic factor
Effect, cannot achieve quick vascularization and the reparation of inside configuration.
The 3D printing technique for being usually used in biology manufacture at present includes mainly Fused Deposition Modeling (FDM), selective laser burning
Tie the forming techniques such as (SLS), inkjet printing, laser direct-writing.FDM and SLS is harsh to printing material requirements, and is needed in forming process
Material is heated or melted, not be suitable for the on-demand fixed point printing of the bioactive materials such as cell, growth factor.Ink-jet
Printing technique has higher printing precision and technology maturation, but is only applicable to printing low viscosity material, and when printing suspension
Nozzle is easy to block.The printing precision highest of laser writing technology, or even can realize the operation to individual cells, but print effect
Low, three-dimensional difference of rate etc. limits its application.
On the other hand, if relying only on single nozzle to realize more material printings and realize prepared by high bio-imitability implant
It is even more extremely difficult.Extracellular matrix material requires that certain mechanical support can be provided, and common extracellular matrix material has viscosity height, stream
Move the features such as property is poor.And the printing " ink " of cell and growth factor then requires low, good fluidity of viscosity etc., viscosity is higher to be pressed down
The migration of cell processed and intercellular signal transmit, block cell and the extraneous sustained release for carrying out mass exchange or even growth factor,
To influence the normal functional expression of cell.Simultaneously in view of the particular/special requirement of bioactive materials such as cell, growth factor and
The different demands of printing precision only lean on single printing type to seem awkward.
3D printing forming method in the prior art can not efficiently accomplish the different materials that print characteristic has very big difference
Print job, in particular, cannot be satisfied the preparation requirement of high bio-imitability implant.
Invention content
This application provides a kind of more nozzles to coordinate biological 3D printing forming method, solves or part solves existing skill
3D printing forming method can not efficiently accomplish the print job that print characteristic has the different materials of very big difference in art, especially
It is that cannot be satisfied the technical issues of preparation of high bio-imitability implant requires.
This application provides a kind of more nozzles to coordinate biological 3D printing forming method, the three-dimensional branch for printing multigroup part
Frame, more nozzles are coordinated biological 3D printing forming method and are included the following steps：
It is configured to print multigroup slurry of the three-dimensional rack, all slurries are divided into first according to viscosity height
Class slurry and the second class slurry, the first kind slurry are high viscosity fluidised form moulding material, and the second class slurry is low viscosity
Fluidised form moulding material；
3D printing model is established, the printing path collection of the three-dimensional rack is obtained；
By one or more slurries of the first kind slurry by the electronic extruded type nozzle of corresponding number along institute
It states printing path collection jet printing and forms skeleton；
By one or more slurries of the second class slurry by the pneumatic injecting type nozzle of corresponding number along institute
It states printing path collection to be ejected on the skeleton, the three-dimensional rack is obtained after the completion of printing.
Preferably, the three-dimensional rack is high bio-imitability implant；
The first kind slurry is extracellular matrix material；
The second class slurry is made a living object active material.
Preferably, the first kind slurry is hydroxyapatite-sodium alginate slurry, viscosity is more than or equal to 200pa/s.
Preferably, the component proportion of the hydroxyapatite-sodium alginate slurry is：By percentage to the quality, water, sea
Mosanom, hydroxyapatite three mass ratio be 30：1：28.
Preferably, the preparation process of the hydroxyapatite-sodium alginate slurry is：
Prepare the water, the sodium alginate and the hydroxyapatite according to proportioning；
The sodium alginate is added in the water, is used in combination magnetic stirrer stirring until sodium alginate uniform dissolution；
The powder of the hydroxyapatite is added several times, side edged is stirred with glass plate, while using ultrasonic cleaning
Machine carries out ultrasonic disperse；
The hydroxyapatite configured-sodium alginate slurry preservative film sealed high-temp high-pressure sterilizing is for use.
Preferably, the second class slurry is sodium alginate soln, viscosity is 0~200mpa/s.
Preferably, the preparation process of the sodium alginate soln is：
Sodium alginate powder will be added in distilled water, the mass ratio of the sodium alginate powder and the distilled water is 1:
50, it is stirred with magnetic stirrer up to being uniformly dispersed, obtains the sodium alginate soln；
The sodium alginate soln is placed in autoclave sterilization pot spare after sterilizing.
Preferably, the three-dimensional rack is divided into multiple printable layers by the 3D printing model, each described printable layer
A corresponding printing path, all printing paths form the printing path collection.
Preferably, each described printable layer of the 3D printing model to be carried out to the mark of coordinate points according to setting spacing
Note；
The printing path of each printable layer is expressed as the coordinate form of XY.
Preferably, when the first kind slurry includes multigroup slurry, print system control is multiple described electronic
Extruded type nozzle co-operating, successively prints the skeleton；
When the second class slurry includes multigroup slurry, the print system controls multiple pneumatic injecting types
Nozzle co-operating is successively printed on the skeleton.
One or more technical solutions provided herein, have at least the following technical effects or advantages：
Pass through corresponding electronic extruded type nozzle edge as a result of by the first kind slurry of high viscosity fluidised form moulding material
Printing path collection jet printing forms skeleton；Second class slurry of low viscosity fluidised form moulding material is passed through into corresponding pneumatic injection
Formula nozzle is ejected into along printing path collection on skeleton, and multigroup part of three-dimensional rack is obtained after the completion of printing, print characteristic is made to exist
The different materials of very big difference can effectively print to form more material complex three-dimensional models.In this way, efficiently solving in the prior art
3D printing forming method can not efficiently accomplish the print job that print characteristic has the different materials of very big difference, in particular, nothing
The technical issues of preparation that method meets high bio-imitability implant requires, the continuous printing for realizing high viscosity timbering material are low in
The precise Printing of viscosity cell, growth factor bio-ink makes the soft or hard combination of printed material, meets model labyrinth and hole
The technique effect of the gradient distribution of the demand and ingredient of diameter graded spatially.
Description of the drawings
Fig. 1 is the flow diagram that more nozzles provided in an embodiment of the present invention coordinate biological 3D printing forming method；
Fig. 2 is the structural schematic diagram of print system provided in an embodiment of the present invention；
Fig. 3 is the structural schematic diagram of air-channel system in Fig. 2.
(component that each label represents in diagram is followed successively by：1 is compressed air source, and 2 be pressure reducing valve, and 3 fill for air filtration
It sets, 4,13,14 be gas-guide tube, and 5,6,9,10 be pressure regulator valve, and 7,8 be vacuum generator, and 11,12 be two-bit triplet electricity
Magnet valve, 15,16 be barrel upper cover, and 17,18 be barrel sealing ring, and 19,20 be barrel, and 21,22 be liquid forming
Material, 23,24 be syringe needle, and 25,26 be lead screw, and 27,28 be motor, and 29,30 be guide rail, and 31,32 be that guide rail is slided
Block, 33,34 be nozzle fuselage, and 35,36 be piston, and 37,38,41,42 be piston sealing ring, and 39,40 be heated mould
Block, 43,44 be liquid forming material, and 45,46 be barrel, and 47,48 be barrel bottom end cover, and 49,50 be syringe needle, and 51 are
Substrate, 52 be workbench, and 53 be the first air inlet of two-bit triplet solenoid valve, and 54 be the second air inlet of two-bit triplet solenoid valve
Mouthful, 55 be the first gas outlet of two-bit triplet solenoid valve.)
Specific implementation mode
The embodiment of the present application provides a kind of more biological 3D printing forming methods of nozzle coordination, solves or part solves
3D printing forming method can not efficiently accomplish the print job that print characteristic has the different materials of very big difference in the prior art,
In particular, the technical issues of preparation that cannot be satisfied high bio-imitability implant requires, by by high viscosity fluidised form moulding material
First kind slurry forms skeleton by corresponding electronic extruded type nozzle along printing path collection jet printing；By low viscosity fluidised form at
Second class slurry of shape material is ejected into along printing path collection on skeleton by corresponding pneumatic injecting type nozzle, after the completion of printing
The three-dimensional rack for obtaining multigroup part, the continuous printing and middle low viscosity cell, growth factor for realizing high viscosity timbering material are given birth to
The precise Printing of object ink makes the soft or hard combination of printed material, meet the needs of model labyrinth and bore diameter gradient change with
And the technique effect of the gradient distribution of ingredient spatially.
This application provides a kind of more nozzles to coordinate biological 3D printing forming method, the three-dimensional branch for printing multigroup part
Frame, referring to attached drawing 1, which coordinates biological 3D printing forming method and includes the following steps：
S1：It is configured to multigroup slurry of printing three-dimensional rack, all slurries are divided into the first plasmoid according to viscosity height
Material and the second class slurry, first kind slurry are high viscosity fluidised form moulding material, and the second class slurry is low viscosity fluidised form moulding material.
S2：3D printing model is established, the printing path collection of three-dimensional rack is obtained.
S3：By one or more slurries of first kind slurry by the electronic extruded type nozzle of corresponding number along printing path
Collect jet printing and forms skeleton.
S4：By one or more slurries of the second class slurry by the pneumatic injecting type nozzle of corresponding number along printing path
Collection is ejected on skeleton, and three-dimensional rack is obtained after the completion of printing.
Wherein, the first kind slurry of the above method by corresponding electronic extruded type nozzle along printing path collection jet printing
Form skeleton；Second class slurry of low viscosity fluidised form moulding material is sprayed by corresponding pneumatic injecting type nozzle along printing path collection
It is mapped on skeleton, realizes the essence of the continuous printing and middle low viscosity cell, growth factor bio-ink of high viscosity timbering material
Really printing, makes the soft or hard combination of printed material, meets the needs of model labyrinth and bore diameter gradient variation and ingredient in sky
Between on gradient distribution, be particularly suitable for biology 3D printing demand.
Specific embodiment as one preferred, three-dimensional rack are high bio-imitability implant；First kind slurry is bone matrix
Material；Second class slurry is made a living object active material.Wherein, first kind slurry is hydroxyapatite-sodium alginate slurry, and viscosity is big
In equal to 200pa/s.Second class slurry is sodium alginate soln, and viscosity is 0~200mpa/s.
Further, the component proportion of hydroxyapatite-sodium alginate slurry is：By percentage to the quality, water, alginic acid
Sodium, hydroxyapatite three mass ratio be 30：1：28.The preparation process of hydroxyapatite-sodium alginate slurry is：
Prepare water, sodium alginate and hydroxyapatite according to proportioning.
Sodium alginate is added in the water, is used in combination magnetic stirrer stirring until sodium alginate uniform dissolution.
The powder of hydroxyapatite is added several times, side edged is stirred with glass plate, while with supersonic wave cleaning machine into
Row ultrasonic disperse.
The hydroxyapatite configured-sodium alginate slurry preservative film sealed high-temp high-pressure sterilizing is for use.
Further, the preparation process of sodium alginate soln is：
Sodium alginate powder will be added in distilled water, the mass ratio of the sodium alginate powder and the distilled water is 1:
50, it is stirred with magnetic stirrer up to being uniformly dispersed, obtains the sodium alginate soln.
Sodium alginate soln is placed in autoclave sterilization pot spare after sterilizing.
Further, three-dimensional rack is divided into multiple printable layers by 3D printing model, each printable layer corresponds to a printing
Path, all printing paths form printing path collection.Wherein, by each printable layer of 3D printing model according to setting spacing into
The label of row coordinate points；The printing path of each printable layer is expressed as the coordinate form of XY.
Further, when first kind slurry includes multigroup slurry, the multiple electronic extruded type nozzle associations of print system control
With action, skeleton is successively printed；When the second class slurry includes multigroup slurry, print system controls multiple pneumatic injections
Formula nozzle co-operating, is successively printed on skeleton.
The 3D printing forming method and the printing shaping method that the application is discussed in detail below by specific embodiment make
Print system：
The present embodiment provides a kind of more nozzles to coordinate biological 3D printing forming method, is as follows：
S1：Print the configuration step of slurry
The larger slurry configuration step of viscosity：0.5g ammonium hydroxide is added in 30g distilled water, then is added slurry into distilled water
It is stirred dissolving, being placed on calm shady place after configuration is good stands a period of time, is put into the motor being connected with circuit system and helps
It pushes away in the electronic extruded type nozzle of micro-syringe formula.
The smaller slurry configuration step of viscosity：Slurry is added in 100g distilled water and is stirred dissolving, carries out sterilizing it
It is put into afterwards in the pneumatic injecting type nozzle for the gas pressure boosting micro-injection device formula being connected with pneumatic system.
Slurry configuration can be in accordance with the following steps：
The larger slurry of viscosity is hydroxyapatite-sodium alginate slurry, and preparation method is：30g distilled water is taken, is then added
Enter 1g sodium alginates, with magnetic stirrer stirring until sodium alginate uniform dissolution；Then nano-hydroxy-apatite is added several times
Stone powder, side edged are stirred with glass plate, while carrying out ultrasonic disperse with supersonic wave cleaning machine；It is sealed with preservative film after configuration is good
Autoclave sterilization is for use.
The smaller slurry of viscosity is sodium alginate soln, and preparation method is：100g distilled water is taken, 2g sodium alginate powders are added
End is stirred with magnetic stirrer until being uniformly dispersed；Sodium alginate soln is placed in autoclave sterilization pot spare after sterilizing.
S2：Model 3D printing step
Specially：Model is carried out to the label of coordinate points, the seat that it is XY that entire path, which completely represents, according to certain interval
Then mark form is conducted into printing auxiliary system and exports the identifiable path file of print system again.
S3：By obtained path file, electronic extruded type nozzle carries out holder printing first, when beating for one layer of holder of completion
After print, nozzle rises setting height, then print with the holder in first layer support vertical direction, two layers of chiasma type branch of stroke
Frame.By obtained path file, pneumatic injecting type nozzle is moved to designated position and is printed.
In this embodiment, above-mentioned more nozzles are completed by following print system and coordinates biological 3D printing molding side
Method：
By taking Fig. 2 as an example, Fig. 2 includes 2 sets of pneumatic injecting type printing heads and 2 electronic extruded type printing heads, pneumatically
Injecting type printing head barrel 19,20 and electronic extruded type printing head barrel 45, the installation of 46 horizontal Tiles, further include multiple beat
Print nozzle 23,24,49,50, multiple (such as two shown in Fig. 2) two-position three way magnetic valves 11,12, multiple (such as four shown in Fig. 2
It is a) pressure regulator valve 5,6,9,10, multiple (such as two shown in Fig. 2) vacuum generators 7,8, compressed air source 1, pressure reducing valve 2, air
Filter device 3, pressure regulator valve 5,6,9,10, two-bit triplet solenoid valve 11,12, gas-guide tube 4,13,14, workbench 52 and substrate 51.
The stepper motor 27,28 of electronic extruded type printing head works, and converts motor torque to motive force by lead screw 25,26 at this time
It acts on piston 35,36, power is loaded on liquid forming material 43,44 by piston 35,36, the fluidised form is forced to shape material
Material passes through in the injection to substrate 51 of syringe needle 49,50.The three-dimensional motion of above-mentioned injection and workbench 52 and fixed station, can be in substrate
The a certain layer of three-dimensional rack is printed on 51.
After having printed a certain layer holder, it can make to come from by controlling some solenoid valve (such as 11) in solenoid valve 11,12
The compressed air of compressed air source 1 by pressure reducing valve 2 and air filter 3, into a certain group of gas circuit pressure regulator valve (such as
9), then entered by some corresponding solenoid valve (such as 11), some gas-guide tube (such as 13), the top of the barrel lid (such as 15)
Some barrel (such as 19) forces the fluidised form moulding material with liquid to apply pressure to fluidised form material (such as 29) therein
Drop mode is sprayed to the three-dimensional rack on substrate 51.It can essence by the three-dimensional motion of workbench 52 and fixed station (not shown)
The specific position that the drop is injected in three-dimensional rack is really controlled, to realize the printing of multicomponent three-dimensional rack.Holder it is a certain
After the completion of layer printing, fixed station vertically rises a floor height, and to next layer of profile of print carriage, so cycle is straight
It prints and completes to three-dimensional rack.
The structural schematic diagram of air-channel system that the present invention uses shown in Fig. 3, the gas circuit by compressed air source 1, pressure reducing valve 2,
Filter 3, gas-guide tube 4, pressure regulator valve 5,9, vacuum generator 7 and two-bit triplet solenoid valve 11 form.Compressed air is by subtracting
Pressure valve 2, filter 3 and gas-guide tube load are passed through by the compressed air of pressure regulator valve 9 on solenoid valve 11 on pressure regulator valve 5,9
Air inlet 53 is connected with solenoid valve, and loads compressed air on solenoid valve 5 by after vacuum generator 7 and solenoid valve 11
Second air inlet 54 is connected, and the second air inlet 54 of the two-position three way magnetic valve and gas outlet 55 are in normal open state, first into
Gas port 53 and gas outlet 54 are in normal off state, and what gas-guide tube 13 generated at this time is exactly negative pressure, and problem is salivated to eliminate material.
In addition, loading the time on material by fine-tuning positive/negative-pressure, more stable drop can be generated；When control solenoid valve 11
After energization, in an ON state, and the second air inlet 54 and gas outlet 55 are off for air inlet 53 and gas outlet 54, this
When gas-guide tube 13 generate be positive pressure；By controlling the make-and-break time of solenoid valve 11, control comes out positive and negative from gas-guide tube 13
The time of pressure, then pneumatic injector formula printing head can realize the continuous of printed droplets or interval injection.
Pneumatic injecting type nozzle can be used for spraying low viscosity fluidised form moulding material, and viscosity is generally 0~200mpa/s, such as
Water, sodium alginate soln (more common in biological 3D printing), printing precision can reach 200~300 μm；Electronic extruded type nozzle
It can be used for squeezing out high viscosity fluidised form moulding material, viscosity is generally hundreds of pa/s (being such as larger than equal to 200pa/s), belongs to semisolid
State, such as hydroxyapatite material system, hydroxyapatite-sodium alginate system.Due to pneumatic injecting type nozzle and it is electronic squeeze
It can be multiple to go out formula nozzle, and the liquid forming material type in each pneumatic injecting type nozzle barrel can be different, similar,
Liquid forming material type in each electronic extruded type nozzle barrel can also be different.For example, electronic extruded type nozzle can be with
For printing the holder with some strength, and pneumatic injecting type nozzle is then used to low viscosity moulding material fixed point printing to this
On holder, the printing of multi-component complex threedimensional model is realized.
More nozzles of the present embodiment coordinate biological 3D printing molding and carry out automation control using computer program, control journey
Sequence can individually distribute processing tasks to each nozzle, and carry out the control and coordination between more nozzles, make more nozzles can be with
It is printed simultaneously according to setting program, realizes the printing of more controllable three-dimensional racks of material spatial distribution, disclosure satisfy that at present
The needs that biological 3D printing prints more material complex three-dimensional models.
One or more technical solutions provided herein, have at least the following technical effects or advantages：
Pass through corresponding electronic extruded type nozzle edge as a result of by the first kind slurry of high viscosity fluidised form moulding material
Printing path collection jet printing forms skeleton；Second class slurry of low viscosity fluidised form moulding material is passed through into corresponding pneumatic injection
Formula nozzle is ejected into along printing path collection on skeleton, and multigroup part of three-dimensional rack is obtained after the completion of printing, print characteristic is made to exist
The different materials of very big difference can effectively print to form more material complex three-dimensional models.In this way, efficiently solving in the prior art
3D printing forming method can not efficiently accomplish the print job that print characteristic has the different materials of very big difference, in particular, nothing
The technical issues of preparation that method meets high bio-imitability implant requires, the continuous printing for realizing high viscosity timbering material are low in
The precise Printing of viscosity cell, growth factor bio-ink makes the soft or hard combination of printed material, meets model labyrinth and hole
The technique effect of the gradient distribution of the demand and ingredient of diameter graded spatially.
Above-described specific implementation mode has carried out further the purpose of the present invention, technical solution and advantageous effect
It is described in detail, it should be understood that the foregoing is merely the specific implementation mode of the present invention, is not limited to this hair
Bright, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should be included in the present invention
Protection domain within.
Claims (10)
1. a kind of more nozzles coordinate biological 3D printing forming method, which is characterized in that the three-dimensional rack for printing multigroup part, institute
More biological 3D printing forming methods of nozzles coordination are stated to include the following steps：
It is configured to print multigroup slurry of the three-dimensional rack, all slurries are divided into the first plasmoid according to viscosity height
Material and the second class slurry, the first kind slurry are high viscosity fluidised form moulding material, and the second class slurry is low viscosity fluidised form
Moulding material；
3D printing model is established, the printing path collection of the three-dimensional rack is obtained；
One or more slurries of the first kind slurry are beaten by the electronic extruded type nozzle of corresponding number described in
It prints path set jet printing and forms skeleton；
One or more slurries of the second class slurry are beaten by the pneumatic injecting type nozzle of corresponding number described in
Print path set is ejected on the skeleton, and the three-dimensional rack is obtained after the completion of printing.
2. more nozzles as described in claim 1 coordinate biological 3D printing forming method, which is characterized in that
The three-dimensional rack is high bio-imitability implant；
The first kind slurry is extracellular matrix material；
The second class slurry is made a living object active material.
3. more nozzles as claimed in claim 2 coordinate biological 3D printing forming method, which is characterized in that the first kind slurry
For hydroxyapatite-sodium alginate slurry, viscosity is more than or equal to 200pa/s.
4. more nozzles as claimed in claim 3 coordinate biological 3D printing forming method, which is characterized in that the hydroxy-apatite
The component proportion of stone-sodium alginate slurry is：By percentage to the quality, water, sodium alginate, hydroxyapatite three mass ratio
It is 30：1：28.
5. more nozzles as claimed in claim 4 coordinate biological 3D printing forming method, which is characterized in that the hydroxy-apatite
The preparation process of stone-sodium alginate slurry is：
Prepare the water, the sodium alginate and the hydroxyapatite according to proportioning；
The sodium alginate is added in the water, is used in combination magnetic stirrer stirring until sodium alginate uniform dissolution；
The powder of the hydroxyapatite is added several times, side edged is stirred with glass plate, while with supersonic wave cleaning machine into
Row ultrasonic disperse；
The hydroxyapatite configured-sodium alginate slurry preservative film sealed high-temp high-pressure sterilizing is for use.
6. more nozzles as claimed in claim 2 coordinate biological 3D printing forming method, which is characterized in that the second class slurry
For sodium alginate soln, viscosity is 0~200mpa/s.
7. more nozzles as claimed in claim 6 coordinate biological 3D printing forming method, which is characterized in that the sodium alginate is molten
The preparation process of liquid is：
Sodium alginate powder will be added in distilled water, the mass ratio of the sodium alginate powder and the distilled water is 1:50, it uses
Magnetic stirrer stirring obtains the sodium alginate soln up to being uniformly dispersed；
The sodium alginate soln is placed in autoclave sterilization pot spare after sterilizing.
8. more nozzles as described in claim 1 coordinate biological 3D printing forming method, which is characterized in that the 3D printing model
The three-dimensional rack is divided into multiple printable layers, each described printable layer corresponds to a printing path, all print paths
Diameter forms the printing path collection.
9. more nozzles as claimed in claim 8 coordinate biological 3D printing forming method, which is characterized in that
Each described printable layer of the 3D printing model is carried out to the label of coordinate points according to setting spacing；
The printing path of each printable layer is expressed as the coordinate form of XY.
10. more nozzles as claimed in claim 9 coordinate biological 3D printing forming method, which is characterized in that
When the first kind slurry includes multigroup slurry, the multiple electronic extruded type nozzle collaborations of print system control
Action, successively prints the skeleton；
When the second class slurry includes multigroup slurry, the print system controls multiple pneumatic injecting type nozzles
Co-operating is successively printed on the skeleton.
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| CN109774118A (en) * | 2019-03-21 | 2019-05-21 | 中国科学院福建物质结构研究所 | A method of the mechanical property of enhancing FDM 3D printing product |
| CN110001048A (en) * | 2019-03-04 | 2019-07-12 | 艾伯尔三氐打印技术（重庆）有限公司 | A kind of 3D printing equipment and its Method of printing |
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| CN109774118B (en) * | 2019-03-21 | 2020-12-29 | 中国科学院福建物质结构研究所 | Method for enhancing mechanical property of FDM3D printing part |
| CN109774118A (en) * | 2019-03-21 | 2019-05-21 | 中国科学院福建物质结构研究所 | A method of the mechanical property of enhancing FDM 3D printing product |
| CN110340933A (en) * | 2019-06-28 | 2019-10-18 | 华中科技大学 | A kind of intelligent bionic crawl clamping device based on 4D printing shaping |
| CN110340933B (en) * | 2019-06-28 | 2020-08-28 | 华中科技大学 | Intelligent bionic grabbing and clamping device based on 4D printing forming |
| CN110450405B (en) * | 2019-08-22 | 2021-12-28 | 浙江大学 | Multi-nozzle cooperative biological printing method |
| CN110450405A (en) * | 2019-08-22 | 2019-11-15 | 浙江大学 | More spray heads cooperate with biometric print method |
| CN112743850A (en) * | 2020-12-23 | 2021-05-04 | 华中科技大学鄂州工业技术研究院 | Preparation method of low-temperature biological 3D printing composite stent |
| CN113001986A (en) * | 2021-03-23 | 2021-06-22 | 上海红阵信息科技有限公司 | Method for manufacturing 3D model based on bionic material |
| CN113771358A (en) * | 2021-08-26 | 2021-12-10 | 北京中医药大学 | Three-dimensional printing method and raw materials of traditional Chinese medicine prescription and three-dimensional printing preparation |
| CN115517828A (en) * | 2022-09-06 | 2022-12-27 | 深圳先进技术研究院 | Printing method, printing system, terminal and storage medium of tissue engineering scaffold |
| CN116352018A (en) * | 2023-02-09 | 2023-06-30 | 南京航空航天大学 | Gradient self-adaptive printing shape control method for multi-material composite sand mold |
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