CN108338995A - Purposes of the mushroom volatile oil in preparing antibacterials or food preservative - Google Patents
Purposes of the mushroom volatile oil in preparing antibacterials or food preservative Download PDFInfo
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- CN108338995A CN108338995A CN201810203044.2A CN201810203044A CN108338995A CN 108338995 A CN108338995 A CN 108338995A CN 201810203044 A CN201810203044 A CN 201810203044A CN 108338995 A CN108338995 A CN 108338995A
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- mushroom
- volatile oil
- bacterium
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- staphylococcus aureus
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 48
- 239000000341 volatile oil Substances 0.000 title claims abstract description 31
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 15
- 229940088710 antibiotic agent Drugs 0.000 title claims abstract description 8
- 239000005452 food preservative Substances 0.000 title claims abstract description 8
- 235000019249 food preservative Nutrition 0.000 title claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 32
- 241000191967 Staphylococcus aureus Species 0.000 claims abstract description 16
- 229940079593 drug Drugs 0.000 claims abstract description 12
- 239000003814 drug Substances 0.000 claims abstract description 12
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 claims abstract description 10
- 229960003085 meticillin Drugs 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims abstract description 8
- 241000588769 Proteus <enterobacteria> Species 0.000 claims abstract description 7
- 208000037386 Typhoid Diseases 0.000 claims abstract description 7
- 201000008297 typhoid fever Diseases 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 241000191940 Staphylococcus Species 0.000 claims description 9
- 241000588724 Escherichia coli Species 0.000 claims description 8
- 239000004615 ingredient Substances 0.000 claims description 7
- 241000607768 Shigella Species 0.000 claims description 6
- 241000194032 Enterococcus faecalis Species 0.000 claims description 5
- 241000192125 Firmicutes Species 0.000 claims description 5
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 239000000470 constituent Substances 0.000 claims description 4
- 210000002615 epidermis Anatomy 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000001256 steam distillation Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000002421 anti-septic effect Effects 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 5
- 206010016952 Food poisoning Diseases 0.000 abstract description 4
- 208000019331 Foodborne disease Diseases 0.000 abstract description 4
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 4
- 230000000845 anti-microbial effect Effects 0.000 abstract description 3
- 230000005764 inhibitory process Effects 0.000 abstract description 3
- 238000001228 spectrum Methods 0.000 abstract description 3
- 239000004599 antimicrobial Substances 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 description 11
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 241000194033 Enterococcus Species 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N hexane Substances CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000010606 normalization Methods 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- -1 vinyl compound Chemical class 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 206010012741 Diarrhoea haemorrhagic Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001360526 Escherichia coli ATCC 25922 Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 235000001715 Lentinula edodes Nutrition 0.000 description 1
- 240000000599 Lentinula edodes Species 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 241000607764 Shigella dysenteriae Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical group [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 241000746966 Zizania Species 0.000 description 1
- 235000002636 Zizania aquatica Nutrition 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 235000010603 pastilles Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229940007046 shigella dysenteriae Drugs 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000010353 tongjiang Substances 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides purposes of the mushroom volatile oil in preparing antibacterials or food preservative.The experimental results showed that mushroom volatile oil antimicrobial spectrum is wider, more preferably to the inhibition of drug tolerant bacteria, especially methicillin-resistant staphylococcus aureus, small toxicity, extraction is convenient, and exploitation has great application prospect at drug；Mushroom volatile oil causes the bacterium of food spoilage to have certain bacteriostatic activity these, such as：Proteus, typhoid bacillus etc., therefore it is preventing food spoilage, preventing food poisoning etc. has certain application potential, has and is used as natural antiseptic agent potentiality to be exploited.
Description
Technical field
The present invention relates to purposes of the mushroom volatile oil in preparing antibacterials or food preservative.
Background technology
Mushroom (Lentinus edodes) also known as dried mushroom, mushroom, flower mushroom, fragrant wild rice, are known as " plant queen, " meat or fish in element "
Good reputation.In addition to this, mushroom also has good medical value.Modern research suggests that mushroom have it is antitumor, anti-oxidant, carry
The effects that high immunity.Lentnus edodes amount, export volume and the consumption figure in China are respectively positioned on first place in the world.But the mushroom product in China
Mainly based on dry, fresh goods, product form is single, and added value is low, and intensive processing level more falls behind.
As China's mushroom industry size of synthesis is continuously improved, comprehensive utilization degree and intensive processing level are gradually closed
Note.Mushroom mainly contains polysaccharide, nucleosides, vitamin, amino acid and a small amount of volatile materials etc..Research is concentrated mainly on perfume (or spice) at present
In terms of mushroom polysaccharide, and develop corresponding product such as mushroom injection etc..And the pharmacological action of other components such as volatile ingredient
It is not illustrated, and when developing mushroom Related product, often abandons volatile ingredient, serious waste resource.
Invention content
The purpose of the present invention is to provide purposes of the mushroom volatile oil in preparing antibacterials or food preservative.
Further, the bacterium is bacterium.
Further, the bacterium is Gram positive bacteria or Gram negative bacterium.
Further, the Gram positive bacteria includes staphylococcus aureus, staphylococcus epidermis or enterococcus faecalis；Institute
It includes Escherichia coli, Shigella, proteus, typhoid bacillus to state Gram negative bacterium.
Further, the staphylococcus aureus is staphylococcus aureus ATCC25923.
Further, the bacterium is drug tolerant bacteria, it is preferable that the drug tolerant bacteria is methicillin-resistant staphylococcus
Staphylococcus；It is highly preferred that the drug tolerant bacteria is methicillin-resistant staphylococcus aureus ATCC43300.
Further, the mushroom volatile oil is to prepare gained by following methods：
1) mushroom is weighed, crushes, is extracted with steam distillation, collects distillate；
2) distillate obtained by step 1) is taken, is removed water to get oil-like extracts.
Further, in step 2), the water removal phase refers to being removed water with anhydrous sodium sulfate.
The present invention also provides a kind of antibacterials, it is using mushroom volatile oil as active constituent, in addition can pharmaceutically connect
The preparation that the auxiliary material or complementary ingredient received are prepared.
The present invention also provides a kind of food preservatives, it is the food being prepared as active constituent using mushroom volatile oil
Preservative.
The experimental results showed that mushroom volatile oil antimicrobial spectrum is wider, to drug tolerant bacteria, especially methicillin-resistant staphylococcus
More preferably, small toxicity, extraction is convenient for staphylococcic inhibition, and exploitation has great application prospect at drug；Mushroom volatilizees
Oil causes the bacterium of food spoilage to have certain bacteriostatic activity these, such as：Proteus, typhoid bacillus etc., therefore it is preventing to eat
Object is rotten, and preventing food poisoning etc. has certain application potential, has and is used as natural antiseptic agent potentiality to be exploited.
Obviously, the above according to the present invention is not being departed from according to the ordinary technical knowledge and customary means of this field
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The specific implementation mode of form by the following examples remakes further specifically the above of the present invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.It is all to be based on the above of the present invention
The technology realized all belongs to the scope of the present invention.
Specific implementation mode
Embodiment 1
1. material and instrument
1.1 material
Crude drug source：Dried thin mushroom is originated from Sichuan Province Tongjiang mountain overlord's wild food Co., Ltd.
1.2 reagent
N-hexane (Sinopharm Chemical Reagent Co., Ltd., lot number：T20100928)；Anhydrous sodium sulfate (Chinese medicines group
Learn reagent Co., Ltd, lot number：20140114) it is that analysis is pure；DMSO (dimethyl sulfoxide, Chengdu Ke Long chemical reagents factory, batch
Number：2015110201)；Water is distilled water；MH agar (Hangzhou Basebio Bio-tech Co., Ltd., lot number：20160602001).
Escherichia coli ATCC25922, staphylococcus aureus ATCC25923, ATCC43300 are given by Sichuan Province's antibacterial research,
Remaining bacterial strain, which is all from, to be clinically separated.
1.3 instrument
Light oil extractor (Sichuan Shu Niu glass apparatus Co., Ltd), MIT-P bacterium multiple spots are inoculated with instrument, and (Japan's assistant is public between long
Department), electric jacket (Beijing Zhong Xing great achievements Instrument Ltd.), (Shanghai Lang Xuan is real for DHP-9050B Intelligent electric heatings constant incubator
Test equipment Co., Ltd), HX-200 type high speeds medicinal herb grinder (Zhejiang Province Yongkang City small stream bank hardware medical instrument factory),
Agilent7890A-5975C gas chromatograph-mass spectrometer (GC-MS)s, Agilent GC-MSD work stations (Agilent company of the U.S.)；
Sartorius BP211D AG electronic balances (Sartorius AG).
2. experimental method
The extraction of 2.1 mushroom volatile oil
Vapor evaporates distiller extraction method：Mushroom is ground into dry powder (grain size is 180-230 μm), it is dry to weigh 400g mushrooms
Powder is fitted into 20000ml steam distillation extractors, and 4000g distilled water is added, and is heated to slightly boiling with electric jacket, is extracted 8h, receives
Collect distillate, appropriate anhydrous sodium sulfate removing lower part distilled water is added and both obtains oil-like extracts 0.3mL, 4 DEG C are positioned over after sealing
Refrigerator preserves.Calculate recovery rate.Recovery rate (%)=oily liquids volume/example weight × 100.
It takes 20 μ L of above-mentioned volatile oil to be dissolved in 1mL n-hexanes, is packed into sample injection bottle, be GC-MS determination samples.
2.2 mushroom Analysis of Essential Oil Components
GC-MS operating conditions：Column model HP-5MS capillary mass spectrums column (0.25 μm of the μ ms of 30m × 250)；Carrier gas is
Helium, 1.0 DEG C of min of volume flow-1；Temperature programming, 60 DEG C of initial temperature is taken to rise to 110 DEG C with 10 DEG C of min-1, with
15℃·min-1140 DEG C of holding 10min are risen to, with 10 DEG C of min-1180 DEG C of holding 3min are risen to, with 10 DEG C of min-1It rises to
250 DEG C are kept for 5 DEG C, with 10 DEG C of min-1Rise to 290 DEG C；Split sampling, 1 ︰ 5 of split ratio；1 μ L of sample size；Scan pattern：It sweeps entirely
It retouches；Ionization mode EI, electron energy 70eV, 230 DEG C of ion source temperature.
Quantitative and qualitative method:Computer library searching of the corresponding mass spectrogram of each chromatographic peak through combined instrument carries out qualitative, matter
Modal data is that NIST14 composes library；The relative amount of each component is to use peak area normalization method meter by computer according to total ion current figure
It calculates and obtains.
2.3 mushroom volatile oil antibacterial activities are studied
2.3.1 Spawn incubation
Antibacterial experiment bacterial strain is staphylococcus aureus etc., respectively with scribing line on the tablet of sterilized agar medium
Bacterium needed for method inoculation, and tablet is put into electro-heating standing-temperature cultivator, it is cultivated at 37 DEG C.
2.3.2 the preparation of sample to be tested
Accurate weighing mushroom volatile oil and the initial concentration that 75mg/ml is configured to DMSO are used in combination doubling dilution to configure 4
A concentration gradient, concentration is respectively 37.5mg/mL, 18.75mg/mL, 9.375mg/mL, 4.6875mg/mL, then takes 1mLDMSO
With setting to 0 concentration samples.It takes the sample of each concentration of 1mL to be added in culture dish successively, 14mL culture mediums is then added, mixing is made
The concentration of pastille culture medium be respectively that 2. 3. 4. 0.625mg/mL is 5. by 1.25mg/mL by 2.5mg/mL for 1. 5mg/mL
0.3125mg/mL⑥0mg/mL。
2.3.3 antibacterial experiment
2mL physiological saline is added in each test tube, bacterium used in experiment is transferred to test tube with oese from culture dish
In, turbidimetry counts, and 1.5 × 106CFU/mL of bacterial concentration is adjusted to sterile saline.Add in the inoculation test tube of point sample instrument
Enter 2mL physiological saline, then mixing in inoculation test tube is added in the bacterium solution for taking 20 μ L to mix up concentration respectively, strain is connect with point sample instrument
Kind is put into 37 DEG C of cultures in constant incubator and for 24 hours, observes result together on culture medium.
3. experimental result
The extraction result of 3.1 mushroom volatile oil
0.3mL clear, colorless oily liquid is obtained altogether, it is irritant.Recovery rate=0.3mL/400g × 100%=
0.075%.
3.2 mushroom Analysis of Essential Oil Components results
Total ion of the mushroom volatile oil through GC-MS separation analyses, passes through NIST14 mass spectrometric datas system retrieval and artificial solution
Analysis, and each component percentage contents are measured using areas of peak normalization method, by each compound integral area list, amount to 19 kinds
Compound the results are shown in Table 1.
1 mushroom Analysis of Essential Oil Components of table
It is sulfur-bearing class compound that content is highest in 19 components identified as can be seen from Table 1, accounts for total ingredient
29.44%, it is secondly alkane derivative, accounts for the 23.41% of total ingredient, vinyl compound and aldehyde compound respectively account for total ingredient
11.92% and 8.16%.
3.3 mushroom volatile oil bacteriostatic activity results
Mushroom volatile oil is antibacterial to the results are shown in Table 2.
2 mushroom volatile oil In Vitro Bacteriostatic result of table
Note："×" indicates that this experimental concentration does not show Mlc, 6. the culture medium inoculated of number concentration strain,
But sample is not added, has grown bacterium；Blank group does not access strain, does not grow bacterium, meets positive control and negative control
As a result.
Mushroom volatile oil is to methicillin-resistant staphylococcus aureus, methicillin-resistant staphylococcus as can be seen from the above table
Staphylococcus in clinic separation strains, S. atreus clinical separation strains 1. 3. 4., Escherichia coli standard bacteria, Escherichia coli it is clinical
Separation strains 1., staphylococcus epidermis, proteus and typhoid bacillus have smaller bacteriostasis, concentration need to reach 5mg/mL, right
The Mlc of S. atreus clinical separation strains 2. be 1.25mg/mL, to S. atreus clinical separation strains 5.,
Enterococcus clinical separation strain 2., Escherichia coli clinical separation strain 3., Escherichia coli clinical separation strain 2. do not show fungistatic effect,
It is 0.625mg/mL to Shigella, the Mlc of enterococcus clinical separation strain 3., 1. 4. 5. to enterococcus clinical separation strain
Mlc be 0.3125mg/mL.
In conclusion mushroom volatile oil antibacterial activity test is the result shows that the antimicrobial spectrum of mushroom volatile oil is wider, to drug resistance
The inhibition of property bacterium, especially methicillin-resistant staphylococcus aureus more preferably, to staphylococcus epidermis, proteus,
Typhoid bacillus, staphylococcus aureus, Escherichia coli have certain inhibiting effect, have stronger suppression to enterococcus faecalis, Shigella
It makes and uses, be applied to have in drug and use foreground well.It is proteus, typhoid bacillus, staphylococcus aureus, resistance to
Methicillin staphylococcus aureus, Escherichia coli, enterococcus faecalis, Shigella are mostly pathogenic entero becteria, food can be caused to become
Matter and food poisoning.Enterococcus faecalis category gram-positive bacteria, it is considered that enterococcus is probiotics, but discovered in recent years its essence
For conditioned pathogen, caused nosocomial infection is in increasing trend.Shigella is also referred to as shigella dysenteriae, and it is thin to belong to Gram-negative
Intracellular pathogenic bacteria mainly encroach on colon, cause inflammation and form ulcer, can cause frequent mucus bloody diarrhea.This research
Show that mushroom volatile oil causes the bacterium of food spoilage to have certain antibacterial activity these, therefore it is preventing food spoilage, prevents
Food poisoning etc. has certain application potential.
Claims (10)
1. purposes of the mushroom volatile oil in preparing antibacterials or food preservative.
2. purposes according to claim 1, it is characterised in that：The bacterium is bacterium.
3. purposes according to claim 2, it is characterised in that：The bacterium is that Gram positive bacteria or Gram are negative
Bacterium.
4. purposes according to claim 3, it is characterised in that：The Gram positive bacteria include staphylococcus aureus,
Staphylococcus epidermis or enterococcus faecalis；The Gram negative bacterium includes Escherichia coli, Shigella, proteus, typhoid fever bar
Bacterium.
5. purposes according to claim 4, it is characterised in that：The staphylococcus aureus is staphylococcus aureus
ATCC25923。
6. purposes according to claim 3 or 4, it is characterised in that：The bacterium is drug tolerant bacteria, it is preferable that described
Drug tolerant bacteria is methicillin-resistant staphylococcus aureus；It is highly preferred that the drug tolerant bacteria is that methicillin-resistant is golden yellow
Color staphylococcus A TCC43300.
7. according to claim 1~6 any one of them purposes, it is characterised in that：The mushroom volatile oil is by following methods
Prepare gained：
1) mushroom is weighed, crushes, is extracted with steam distillation, collects distillate；
2) distillate obtained by step 1) is taken, is removed water to get oil-like extracts.
8. purposes according to claim 7, it is characterised in that：In step 2), the water removal phase refers to using anhydrous slufuric acid
Sodium removes water.
9. a kind of antibacterials, it is characterised in that：It is using mushroom volatile oil as active constituent, in addition pharmaceutically acceptable auxiliary
The preparation that material or complementary ingredient are prepared.
10. a kind of food preservative, it is characterised in that：It is the food antiseptic being prepared as active constituent using mushroom volatile oil
Agent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169734A (en) * | 2018-09-21 | 2019-01-11 | 北京弘纳绿源科技发展有限公司 | A kind of plant source extraction bactericidal deodorant for animal husbandry sterilizing |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102908369A (en) * | 2012-10-24 | 2013-02-06 | 江苏博杰特机电有限公司 | Process for preparing shiitake extract |
-
2018
- 2018-03-13 CN CN201810203044.2A patent/CN108338995A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102908369A (en) * | 2012-10-24 | 2013-02-06 | 江苏博杰特机电有限公司 | Process for preparing shiitake extract |
Non-Patent Citations (4)
| Title |
|---|
| CI´NTIA SORANE GOOD KITZBERGER等: "Antioxidant and antimicrobial activities of Shiitake extracts obtained by organic solvents and supercritical fluids", 《JOURNAL OF FOOD ENGINEERING》 * |
| 张芊等: "《2013年湖北省暨武汉微生物学会会员代表大会暨学术年会》", 21 November 2013 * |
| 杨开等: "香菇精的提取与成分分析", 《中国调味品》 * |
| 薛梅等: "香菇风味物质形成过程的研究进展", 《食药用菌》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109169734A (en) * | 2018-09-21 | 2019-01-11 | 北京弘纳绿源科技发展有限公司 | A kind of plant source extraction bactericidal deodorant for animal husbandry sterilizing |
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