CN108323513A - A kind of fruits and vegetables coloring upgrading growth promoting liquor - Google Patents
A kind of fruits and vegetables coloring upgrading growth promoting liquor Download PDFInfo
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- CN108323513A CN108323513A CN201810245429.5A CN201810245429A CN108323513A CN 108323513 A CN108323513 A CN 108323513A CN 201810245429 A CN201810245429 A CN 201810245429A CN 108323513 A CN108323513 A CN 108323513A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
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Abstract
The invention discloses a kind of fruits and vegetables to colour upgrading growth promoting liquor, and preparing raw material includes:Oligopeptide, Potassium Chitosan Phosphate, edible ethanol, edible acetic acid, surplus are pure water;Preparation method includes:Oligopeptide and pure water, edible ethanol are mixed, oligopeptide solution is obtained;Oligosaccharide is put into oligopeptide solution to the oligopeptide solution for the Potassium Chitosan Phosphate for obtaining low molecular weight section;Polysaccharide is put into the oligopeptide solution that above-mentioned solution is mixed to get the Potassium Chitosan Phosphate of combined molecular weight section;Edible acetic acid is added in above-mentioned solution and colours upgrading growth promoting liquor to get the fruits and vegetables.Fruits and vegetables coloring upgrading growth promoting liquid energy systematicness of the present invention adjusts the physiological activity of crop itself, is allowed to develop towards healthier direction;It is agriculturally applying, can not only solve outstanding problem present in Fruits production, but also strong technical guarantee can be provided for the development of modern agriculture.
Description
Technical field
The present invention relates to the cultivation fields of fruit tree, vegetables, and in particular to a kind of fruits and vegetables coloring upgrading growth promoting liquor.
Background technology
Since the 1970s, Fruits production field largely uses pesticide, chemical fertilizer, growth regulator, fruit yield to obtain
To being substantially improved, but fruit quality declines, and tree body aging is fast, especially in terms of fruit color, seriously affects the income of orchard worker, adds
Unreasonable predation formula tillage method, keep tree body prematurely senile, seriously threaten the development of China's Fruit industry.Apple, grape,
The Fruit industries problems faced such as citrus is especially prominent.Currently on the market specially solve fruit color, tree body aging product very
Few, mainly based on nutrient, the result is that more supplementing, tree vigo(u)r is weaker, and fruit quality more declines.
Chinese patent CN201710305845.5 discloses a kind of method of citrusfruit increasing sugar coloring and anti-pericarp implosion,
Using 98% original powder of sugar content increase of fruits colorant, turns orange-yellow by green in fruit, by orange-yellow turns two growth hairs of aubergine again
Educating the stage respectively sprays 2~3 times;Foliage-spray calcium nitrate is taken and starts within 7~10 days before blooming to spray 1 time, every 7 after full-bloom stage~
It sprays within 10 days 1 time, continuous spray 2 times;Blade face imposes boric acid or borax, starts within 7~10 days before blooming spray for the first time, every after full-bloom stage
It sprays within 7~10 days 1 time, continuous spray 2 times.The fertilizer SPRING WHEAT BEFORE AND AFTER FLOWERING tree crown such as calcium, boron sprays 3 times totally comprehensively.It is comprehensive by the above blade face
After conjunction measure processing, citrus fruit face and pulp colour are preferable, and fruit interior quality (soluble solid and citric acid content) is all
There is preferable improvement.But the patent increases fruit " face value " using pigment, such product, which is eaten for a long time, will seriously endanger human body
Health.
Chinese patent CN201611087351.6 discloses a kind of method coloured to strawberry fruit, using red indigo plant
LED light colours strawberry fruit;By the strawberry after blooming 6~8 days using red and blue LED light processing 18~28 days, locate daily
Manage 15~17h;Wherein, the luminous intensity of red and blue LED light is 5400~5600lx.Specific light quality, light intensity, light application time and
Under the conditions of direction of illumination, strawberry fruit can be promoted to colour in advance, improve its anthocyanidin content.But the patent red and blue LED light
Color limitation, economic cost is high, and effect is general.
Therefore, it is necessary in view of the above problems, offer one kind safely and effectively, can promote fruit color, improve interior quality, together
When can also promote the ecological product of plant growth.
Invention content
The present invention is in view of the above-mentioned problems, offer one kind safely and effectively, can promote fruit color, improve interior quality, simultaneously
Also the fruits and vegetables of plant growth can be promoted to colour upgrading growth promoting liquor.
Technical solution is used by the present invention solves the above problems:A kind of fruits and vegetables coloring upgrading growth promoting liquor, prepares fruits and vegetables
Colour upgrading growth promoting liquor raw material and its mass percent include:Oligopeptide 1%~5%, Potassium Chitosan Phosphate 0.6%~
3%, edible ethanol 1.5%~3%, edible acetic acid 0.6%~2%, surplus is pure water;Wherein, Potassium Chitosan Phosphate is
The composition of the poly sugar derivatives of the oligosaccharide derivatives of molecular weight 200Dal~5000Dal and the dal of 100,000 Dal~300,000;
Fruits and vegetables colour the preparation method of upgrading growth promoting liquor, include the following steps:
Processing 30min~60min is mixed, then in the oligopeptide of the mass percent and pure water by step 1
Processing 30min is mixed in the edible ethanol for adding the mass percent, obtains oligopeptide solution;
Step 2 puts into the Potassium Chitosan Phosphate that the molecular weight of the mass percent is 200Dal~5000Dal
In the oligopeptide solution obtained to step 1, processing 30min is mixed, obtains the Potassium Chitosan Phosphate of low molecular weight section
Oligopeptide solution;
Step 3 puts into the Potassium Chitosan Phosphate that the molecular weight of the mass percent is the Dal of 100,000 Dal~300,000
The oligopeptide solution of the Potassium Chitosan Phosphate of the low molecular weight section obtained to step 2, feeds intake while stirring, after feeding intake,
Remix stir process 30min;Obtain the oligopeptide solution of the Potassium Chitosan Phosphate of combined molecular weight section;
Step 4, in the case of stirring, the Potassium Chitosan Phosphate of the combined molecular weight section described in step poly- three it is low
In poly- peptide solution, the edible acetic acid of the mass percent is added, continues stirring 60min~90min and is coloured to get the fruits and vegetables
Upgrading growth promoting liquor.
Further, prepare fruits and vegetables coloring upgrading growth promoting liquor raw material and its mass percent include:Oligopeptide 2%, shell
Glycan phosphate potassium 2%, 95% edible ethanol 2.5%, 99% edible acetic acid 1.5%, surplus is pure water.
The fruits and vegetables coloring upgrading growth promoting liquid energy systematicness of the application adjusts the physiological activity of crop itself, is allowed to towards more strong
The direction of health is developed.It is agriculturally applying, can not only solve fruit color outstanding problem present in Fruits production, Er Qieke
To provide strong technical guarantee for the development of modern agriculture.Agricultural production is allowed to reduce to chemical pesticide, chemical fertilizer and various adjustings
The dependence of agent enables traditional agriculture ecology to repair, while also actively implementing what country proposed《To the year two thousand twenty fertilizer application amount zero
Increase action scheme》、《To the year two thousand twenty Pesticide use amount zero growth rate action scheme》With《Carry out fruit and vegetable tea organic fertilizer replacing fertilizer row
Dynamic scheme》.
Molecular weight section is the meaning of the molecular weight within the scope of some, is indispensable one in chitosan field
Word, the chitosan of different molecular weight section have the function of different:
Chitosan oligosaccharide, molecular weight 200Dal~2000Dal, sugar subnumber 1~10;
Oligosaccharide, molecular weight 2000Dal~6000Dal, sugar subnumber 10~30;
Polysaccharide, the Dal of molecular weight 6000Dal~100,000, sugar subnumber 30~500;
Recombination chitosan is the requirement according to different efficacies, is prepared into the chitosan of different molecular weight section is compound by difference
Recombination chitosan that molecular weight section forms, that molecular weight distribution is wider, this chitosan function is more prominent, effect becomes apparent from, makees
With significantly;The molecular weight section that the application chooses highlights its colouring function, while also having taken into account it and having promoted plant growth and carry
The function of high fruit-vegetable quality;
Slowly it can hydrolyze or degrade in water and in acid solution due to chitosan, its molecular weight ratio is made to change,
Its effect thus is influenced, so being protected to its functional group by derivatization, avoids its hydrolysis, is degraded;Since chitosan exists
It is often combined with polypeptide in organism, to ensure its stability and physiological function, therefore the application selects micromolecular water dissolubility more
Poly- peptide in formula, both ensures the stabilization of formula, in turn ensures the stabilization of its physiological activity (formula field efficacy).While its
Promote plant growth;The addition of edible acetic acid and edible ethanol is also to provide a large amount of carboxyl and hydroxyl for formula, ensures shell
The stability of glycan phosphate potassium agent structure.
Further, oligopeptide is soybean oligopeptide of the molecular weight in 180Dal~1000Dal.
Further, Potassium Chitosan Phosphate is:To avenge the back skin of crab as raw material, using shell made of conventional method
Polysaccharid derivative.
Further, the mass ratio of oligosaccharide and polysaccharide is 1~3:7~9.
Further, the volume fraction of edible ethanol is 90%~95%.
Further, edible acetic acid volume fraction is 90%~99%.
Further, the method for administration of fruits and vegetables coloring upgrading growth promoting liquor is:The fruit tree growth phase applies 3 times~6 times, interval 15
It~20 days;Vegetables, 2 times~3 times melon, interval 5 days~7 days.
It is an advantage of the invention that:
1. the fruits and vegetables coloring upgrading growth promoting liquor of the present invention can induce the expression of plant pigmentation gene, various in activated plant body
The activity of enzyme, system solves the problems, such as that fruit color and fruit face are repaired from source;
2. the fruits and vegetables coloring upgrading growth promoting liquid energy systematicness of the present invention adjusts the physiological activity of crop itself, it is allowed to towards more
The direction of health is developed, and improves fruit interior quality when to achieve the effect that;
3. the fruits and vegetables coloring upgrading growth promoting liquor of the present invention in fruit coloring etc. in terms of, exterior quality in addition to having outstanding performance
Outside, also shown in fruit and vegetable the speed of growth it is fast, it is solid it is more, disease-resistant, degeneration-resistant it is strong, increase the excellent specific properties such as yield, be it in agriculture
Industry field broader practice is laid a good foundation;
4. the present invention prepares fruits and vegetables using recombination chitosan derivative and colours upgrading growth promoting liquor, and has properly selected difference
Molecular weight section combines, and highlights its colouring function, while also having taken into account its function of promoting plant growth and improve fruit-vegetable quality,
The formula combination of reasonable science ensure that the stability of its effect.
Description of the drawings
It forms part of this specification, show embodiment party of the invention for further understanding the attached drawing of the present invention
Case, and be used for illustrating preparation flow of the invention together with specification.In the accompanying drawings:
Fig. 1 is C31 processing of the present invention influences schematic diagram to fruit value of chromatism;
Fig. 2 is C31 processing of the present invention influences schematic diagram to fruit finish;
Fig. 3 is C31 processing of the present invention influences schematic diagram to fruit fruit shape index;
Fig. 4 is C31 processing of the present invention to fruit SOD value effect schematic diagrames;
Fig. 5 is C31 processing of the present invention to fruit POD value effect schematic diagrames;
Fig. 6 is C31 processing of the present invention to fruit PPO value effect schematic diagrames;
Fig. 7 is the fruit soluble sugar of the citrus orchard in the Wuming area of Guangxi Zhuang Autonomous Region Nanning City of C31 processing of the present invention
Testing result.
Specific implementation mode
The embodiment of the present invention is described in detail below in conjunction with attached drawing, but the present invention can be defined by the claims
Implement with the multitude of different ways of covering.
Embodiment 1
A kind of fruits and vegetables coloring upgrading growth promoting liquor, prepares the raw material and its mass percent packet of fruits and vegetables coloring upgrading growth promoting liquor
It includes:Oligopeptide 1%, Potassium Chitosan Phosphate 0.6%, edible ethanol 1.5%, edible acetic acid 0.6%, surplus are pure water;Its
In, oligopeptide is soybean oligopeptide of the molecular weight in 180Dal~250Dal;Potassium Chitosan Phosphate is:Outside back to avenge crab
Shell is raw material, using chitosan derivatives made of conventional method;Potassium Chitosan Phosphate is molecular weight 200Dal~800Dal
100,000 Dal of oligosaccharide derivatives and molecular weight poly sugar derivatives composition;Oligosaccharide derivatives derive with polysaccharide
The mass ratio of object is 1:9;The volume fraction of edible ethanol is 90%;Edible acetic acid volume fraction is 90%.
The preparation method of above-mentioned fruits and vegetables coloring upgrading growth promoting liquor, includes the following steps:
The oligopeptide of above-mentioned mass percent and pure water are mixed processing 30min, then added by step 1
Processing 30min is mixed in the edible ethanol for stating mass percent, obtains oligopeptide solution;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 200Dal is put into step 1 and obtained by step 2
To oligopeptide solution in, processing 30min is mixed, the oligopeptide for obtaining the Potassium Chitosan Phosphate of low molecular weight section is molten
Liquid;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 100,000 Dal is put into step 2 by step 3
The oligopeptide solution of the Potassium Chitosan Phosphate of obtained low molecular weight section, feeds intake while stirring, after feeding intake, remixes and stirs
Mix processing 30min;Obtain the oligopeptide solution of the Potassium Chitosan Phosphate of combined molecular weight section;
Step 4, in the case of stirring, the above-mentioned combined molecular weight section of step poly- three Potassium Chitosan Phosphate it is low
In poly- peptide solution, the edible acetic acid of above-mentioned mass percent is added, continues to stir 60min to get fruits and vegetables coloring upgrading rush
Long liquid.
Fruits and vegetables coloring upgrading growth promoting liquor method of administration be:The fruit tree growth phase applies 3 times, is spaced 15 days.
The fruits and vegetables of the present embodiment colour upgrading growth promoting liquor, can make that fruit color and luster etc. is external, interior quality is greatly changed
It is kind, effectively solve the outstanding problem that current fruit industry faces.
Embodiment 2
A kind of fruits and vegetables coloring upgrading growth promoting liquor, prepares the raw material and its mass percent packet of fruits and vegetables coloring upgrading growth promoting liquor
It includes:Oligopeptide 5%, Potassium Chitosan Phosphate 3%, edible ethanol 3%, edible acetic acid 2%, surplus are pure water;Wherein, oligomeric
Peptide is soybean oligopeptide of the molecular weight in 850Dal~1000Dal;Potassium Chitosan Phosphate is:Back skin to avenge crab is original
Material, using chitosan derivatives made of conventional method;Potassium Chitosan Phosphate is the low of molecular weight 4000Dal~5000Dal
Polysaccharid derivative and molecular weight are the composition of the poly sugar derivatives of 300,000 Dal;Oligosaccharide derivatives and poly sugar derivatives
Mass ratio be 3:7;The volume fraction of edible ethanol is 95%;Edible acetic acid volume fraction is 99%.
The preparation method of above-mentioned fruits and vegetables coloring upgrading growth promoting liquor, includes the following steps:
The oligopeptide of above-mentioned mass percent and pure water are mixed processing 60min, then added by step 1
Processing 30min is mixed in the edible ethanol for stating mass percent, obtains oligopeptide solution;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 5000Dal is put into step 1 by step 2
In obtained oligopeptide solution, processing 30min is mixed, the oligopeptide for obtaining the Potassium Chitosan Phosphate of low molecular weight section is molten
Liquid;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 300,000 Dal is put into step 2 by step 3
The oligopeptide solution of the Potassium Chitosan Phosphate of obtained low molecular weight section, feeds intake while stirring, after feeding intake, remixes and stirs
Mix processing 30min;Obtain the oligopeptide solution of the Potassium Chitosan Phosphate of combined molecular weight section;
Step 4, in the case of stirring, the above-mentioned combined molecular weight section of step poly- three Potassium Chitosan Phosphate it is low
In poly- peptide solution, the edible acetic acid of above-mentioned mass percent is added, continues to stir 90min to get fruits and vegetables coloring upgrading rush
Long liquid.
Fruits and vegetables coloring upgrading growth promoting liquor method of administration be:Vegetables 3 times are spaced 7 days.
Embodiment 3
A kind of fruits and vegetables coloring upgrading growth promoting liquor, prepares the raw material and its mass percent packet of fruits and vegetables coloring upgrading growth promoting liquor
It includes:Oligopeptide 3%, Potassium Chitosan Phosphate 1.8%, edible ethanol 2.25%, edible acetic acid 1,.3%, surplus is pure water;
Wherein, oligopeptide is soybean oligopeptide of the molecular weight in 600Dal~800Dal;Potassium Chitosan Phosphate is:To avenge the back of crab
Shell is raw material, using chitosan derivatives made of conventional method;Potassium Chitosan Phosphate be molecular weight be 2000Dal~
The oligosaccharide derivatives and molecular weight of 3000Dal are the composition of the poly sugar derivatives of 200,000 Dal;Oligosaccharide derivatives with it is more
The mass ratio of polysaccharid derivative is 2:8;The volume fraction of edible ethanol is 92.5%;Edible acetic acid volume fraction is 94.5%.
The preparation method of above-mentioned fruits and vegetables coloring upgrading growth promoting liquor, includes the following steps:
The oligopeptide of above-mentioned mass percent and pure water are mixed processing 45min, then added by step 1
Processing 30min is mixed in the edible ethanol for stating mass percent, obtains oligopeptide solution;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 2600Dal is put into step 1 by step 2
In obtained oligopeptide solution, processing 30min is mixed, the oligopeptide for obtaining the Potassium Chitosan Phosphate of low molecular weight section is molten
Liquid;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 200,000 Dal is put into step 2 by step 3
The oligopeptide solution of the Potassium Chitosan Phosphate of obtained low molecular weight section, feeds intake while stirring, after feeding intake, remixes and stirs
Mix processing 30min;Obtain the oligopeptide solution of the Potassium Chitosan Phosphate of combined molecular weight section;
Step 4, in the case of stirring, the above-mentioned combined molecular weight section of step poly- three Potassium Chitosan Phosphate it is low
In poly- peptide solution, the edible acetic acid of above-mentioned mass percent is added, continues to stir 75min to get fruits and vegetables coloring upgrading rush
Long liquid.
Fruits and vegetables coloring upgrading growth promoting liquor method of administration be:The fruit tree growth phase applies 6 times, every 20 days.
The fruits and vegetables of the present embodiment colour upgrading growth promoting liquor, can make that fruit color and luster etc. is external, interior quality is greatly changed
It is kind, effectively solve the outstanding problem that current fruit industry faces.
Embodiment 4
A kind of fruits and vegetables coloring upgrading growth promoting liquor, prepares the raw material and its mass percent packet of fruits and vegetables coloring upgrading growth promoting liquor
It includes:Oligopeptide 2%, Potassium Chitosan Phosphate 2%, 95% edible ethanol 2.5%, 99% edible acetic acid 1.5%, surplus are
Pure water;Wherein, oligopeptide is soybean oligopeptide of the molecular weight in 350Dal~450Dal;Potassium Chitosan Phosphate is:With snow
The back skin of crab is raw material, using chitosan derivatives made of conventional method;Potassium Chitosan Phosphate is that molecular weight is
The composition of the oligosaccharide derivatives of 4000Dal~3000Dal and the poly sugar derivatives of 150000Dal;Oligosaccharide derivatives
Mass ratio with poly sugar derivatives is 2.5:7.5;The volume fraction of edible ethanol is 95%;Edible acetic acid volume fraction is
99%.
The preparation method of above-mentioned fruits and vegetables coloring upgrading growth promoting liquor, includes the following steps:
The oligopeptide of above-mentioned mass percent and pure water are mixed processing 40min, then added by step 1
Processing 30min is mixed in the edible ethanol for stating mass percent, obtains oligopeptide solution;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 4000Dal is put into step 1 by step 2
In obtained oligopeptide solution, processing 30min is mixed, the oligopeptide for obtaining the Potassium Chitosan Phosphate of low molecular weight section is molten
Liquid;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 150,000 Dal is put into step 2 by step 3
The oligopeptide solution of the Potassium Chitosan Phosphate of obtained low molecular weight section, feeds intake while stirring, after feeding intake, remixes and stirs
Mix processing 30min;Obtain the oligopeptide solution of the Potassium Chitosan Phosphate of combined molecular weight section;
Step 4, in the case of stirring, the above-mentioned combined molecular weight section of step poly- three Potassium Chitosan Phosphate it is low
In poly- peptide solution, the edible acetic acid of above-mentioned mass percent is added, continues to stir 80min to get fruits and vegetables coloring upgrading rush
Long liquid.
Fruits and vegetables coloring upgrading growth promoting liquor method of administration be:The fruit tree growth phase applies 5 times, is spaced 16 days.
The fruits and vegetables of the present embodiment colour upgrading growth promoting liquor, can make that fruit color and luster etc. is external, interior quality is greatly changed
It is kind, effectively solve the outstanding problem that current fruit industry faces.
Embodiment 5
A kind of fruits and vegetables coloring upgrading growth promoting liquor, prepares the raw material and its mass percent packet of fruits and vegetables coloring upgrading growth promoting liquor
It includes:Oligopeptide 4%, Potassium Chitosan Phosphate 2%, edible ethanol 2.8%, edible acetic acid 1.7%, surplus are pure water;Wherein,
Oligopeptide is soybean oligopeptide of the molecular weight in 250Dal~350Dal;Potassium Chitosan Phosphate is:To avenge the back skin of crab
For raw material, using chitosan derivatives made of conventional method;Potassium Chitosan Phosphate be molecular weight be 1000Dal~
The composition of the poly sugar derivatives of the oligosaccharide derivatives of 2000Dal and 250,000 Dal;Oligosaccharide derivatives derive with polysaccharide
The mass ratio of object is 1.5:8.5;The volume fraction of edible ethanol is 92%;Edible acetic acid volume fraction is 98%.
The preparation method of above-mentioned fruits and vegetables coloring upgrading growth promoting liquor, includes the following steps:
The oligopeptide of above-mentioned mass percent and pure water are mixed processing 50min, then added by step 1
Processing 30min is mixed in the edible ethanol for stating mass percent, obtains oligopeptide solution;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 3000Dal is put into step 1 by step 2
In obtained oligopeptide solution, processing 30min is mixed, the oligopeptide for obtaining the Potassium Chitosan Phosphate of low molecular weight section is molten
Liquid;
The Potassium Chitosan Phosphate that the molecular weight of above-mentioned mass percent is 250,000 Dal is put into step 2 by step 3
The oligopeptide solution of the Potassium Chitosan Phosphate of obtained low molecular weight section, feeds intake while stirring, after feeding intake, remixes and stirs
Mix processing 30min;Obtain the oligopeptide solution of the Potassium Chitosan Phosphate of combined molecular weight section;
Step 4, in the case of stirring, the above-mentioned combined molecular weight section of step poly- three Potassium Chitosan Phosphate it is low
In poly- peptide solution, the edible acetic acid of above-mentioned mass percent is added, continues to stir 70min to get fruits and vegetables coloring upgrading rush
Long liquid.
Fruits and vegetables coloring upgrading growth promoting liquor method of administration be:It melon 3 times, is spaced 7 days.
Experimental example 1
Coloring mechanism and interior quality mechanism of the upgrading growth promoting liquor to fruit are coloured to fruits and vegetables prepared by the embodiment of the present application 4
It is studied, result of study is as shown in table 1.Wherein, the fruits and vegetables coloring upgrading growth promoting liquor that prepared by embodiment 4 is labeled as C31, control
Example is labeled as CK.
Entitled flavanones -3- the hydroxylases of F3H, it is catalyzed trihydroxyflavone alcohol and generates the second light industry bureau flavonols, is Flavonoid substances
The important gene of metabolism and the key structure gene of anthocyanidin pre-synthesis phase.
MYB class transcription factors are to adjust the key factor of anthocyanidin synthesis, it passes through to synthesizing process key enzyme gene table
The synthesis of anthocyanidin is influenced up to the adjusting of amount.I.e. such transcription factor can influence the transcription tune of anthocyanin biosynthetic pathway
Control, causes the activation of anthocyanidin route of synthesis.
Table 1 C31 processing influences result to the gene expression amount of two class of F3H, MYB
Sample | CK | C31 |
F3H | 1 | 4 |
MYB1 | 1 | 1.68 |
MYB2 | 1 | 2.57 |
MYB4 | 1 | 2.02 |
Table 1 the result shows that:C31 processing increases the gene expression amount of two class of F3H, MYB.C31 can induce pigmentation gene
It expresses, the activity of various enzymes in activated plant body.System solves the problems, such as fruit color from source.In annesl C31 early period to flower
Green element pre-synthesis phase gene has stimulation, and the synthesis precursor of anthocyanidin is made to give full expression to.
Experimental example 2
Influence of the upgrading growth promoting liquor to the color and luster of fruit is coloured to fruits and vegetables prepared by the embodiment of the present application 4 to be detected, and is examined
It is as shown in Figure 1 and Figure 2 to survey result.Wherein, the color and luster of fruit is to weigh one of most important index of fruit quality.CIE standard colorimetrics
System refers to a set of color measuring principle, data and computational methods as defined in CIE.L*a*b uniform colour spaces are applied to
In fruit colour product Quality Research.
After Δ E indicates value of chromatism in Fig. 1, and value of chromatism is smaller to illustrate that color is better, therefore C31 is to fruit processing, fruit face
Color is good.
The bigger explanation of L values is brighter in Fig. 2, and after the finish of fruit is higher, therefore C31 is to fruit processing, fruit is bright and clean
Degree is high.
Experimental example 3
The influence of the fruit shape of fruit is tested, test results are shown in figure 3.Wherein, fruit shape index (shape
Index) refer to fruit indulge diameter and transverse diameter ratio.Fruit shape index is one of the quality index of commodity fruit.Apple because of kind not
Together, numerical value is variant, single for Fuji apple, using the stake of fruit height as the important indicator of commercial grade, it is desirable that fruit shape index
In 0.85 or more equal high-grade fruit.
The fruit shape index that Fig. 3 can be seen that the fruit of C31 processing is 0.86, hence it is evident that is better than the fruit shape index of reference examples
0.82, illustrate that C31 has apparent excellent influence to fruit fruit shape.
Experimental example 4
Fruits and vegetables coloring upgrading growth promoting liquor prepared by the embodiment of the present application 4 surveys the mouthfeel of fruit, the influence of flavor
Examination, testing result are as shown in table 2.
2 C31 of table is to the mouthfeel of fruit, the influence of flavor
Project | Hardness | Soluble solid (g/100g) | Titratable acid (%) |
CK | 6.43 | 13.30 | 0.35 |
C31 | 7.34 | 14.59 | 0.38 |
Table 2 the result shows that, C31 processing has a significant effect compared with reference examples to the mouthfeel of fruit, flavor.
Experimental example 5
Influence of the upgrading growth promoting liquor to fruit fruit face, flavor is coloured to fruits and vegetables prepared by the embodiment of the present application 4 to test,
Testing result is as shown in Fig. 4, Fig. 5, Fig. 6.Wherein, superoxide dismutase (SOD) and peroxidase (POD), it is widely distributed
In in various organisms, there is special physiological activity, be the primary substance for removing free radical in organism.It can fight and hinder
It is disconnected to be damaged caused by oxygen radical is cell, and damaged cell is repaired in time, it restores caused by free radical to cell damage.
Polyphenol oxidase (PPO) and a kind of extremely wide oxidoreducing enzyme of distributed in nature and a kind of important terminal oxidase,
Fruit quality, disease-resistant etc. important role.It can also promote fruit to generate various volatile compound (phenol
Substance), and form the basic flavor of fruit, flavour, fragrance.
Fig. 4 is the SOD contents using the fruit and reference examples fruit of C31, the results showed that the SOD using the fruit of C31 contains
Amount is high, and fruit face is finer and smoother.
Fig. 5 is the POD contents using the fruit and reference examples fruit of C31, the results showed that the POD using the fruit of C31 contains
Amount is high, and quality is high, and disease resisting effect is good.
Fig. 6 is the PPO contents using the fruit and reference examples fruit of C31, the results showed that the PPO using the fruit of C31 contains
Amount is high, and taste of fruit is good.
Experimental example 6
Field information effect detection, testing result are carried out to fruits and vegetables coloring upgrading growth promoting liquor prepared by the embodiment of the present application 4
As shown in table 3, Fig. 7.
(1) the old garden of Fuji apple of Wang Hong armies of Shaanxi Province's Weinan City Heyang County towns Jin Yu villages Xia Zhuan, 30 years age of trees, tree vigo(u)r
Weak, rot disease is seriously, low output, fruit is small, commodity rate is low, and 2016,4 C31 are sprayed after bagging, when receiving fruit, each and every one fruit type end
The village, beautiful in colour, flavor is dense, and tree vigo(u)r is clearly better, and is continuing within 2017 C31, and same effect is reappeared in the orchard,
Even if in high temperature in 2017 in the case of being unfavorable for fruit color, the garden fruit shape, color and luster, mouthfeel still keep higher level,
By being detected at index analysis to fruit weight and inherent nutrition, C31 processing is above control, and tree vigo(u)r is remarkably reinforced, and rot disease subtracts
Gently, the villages continuous 2 Nian Weiquan highest procurement price.Dignified using the apple fruit type of C31, beautiful in colour, tree vigo(u)r is good.
Table 3 2017 year October, the 30 old garden data measured of Wang Hong armies Fuji apple
(2) the husky heart of the villages Xianyang, Shanxi province city Chunhua County profit Zhen Yao Wang Hong great waves, 18 years age of trees, 2017, after fruit setting, even
Spray 4 C31, be spaced 10~15 days, under this year high-temperature condition, peach coloring is gorgeous, and taste is fragrant and sweet, cella and back coloring with
There are few difference for sunny side.Former years when picking peach for the second time, have 1/3 peach and feel like jelly, this year is almost without soft peach.And it sets
Gesture is healthy and strong, leaf color is dark green glossy, flexible, and orchard worker is very joyful, and neighbourhood is even more to envy.It is gorgeous using the husky heart coloring of C31,
Tree vigo(u)r is healthy and strong, leaf color is dark green glossy, flexible.
(3) citrus orchard in Wuming area of Guangxi Zhuang Autonomous Region Nanning City colours first 2 months, foliage-spray 1000 in citrus
Times C31, is spaced 15 days, sprays effect after 15 days the 1st time:" blade face thickness is green, fruit color in reflective, effect regulations and parameters ", even spray 3 times
Afterwards, fruit annesl is early, and cella is consistent with peripheral fruit colouring, and top fruit fruit face is fine and smooth, and sugar content is high, and mouthfeel is very good.Using C31's
Citrus fruit face is fine and smooth.Its fruit soluble sugar testing result as shown in fig. 7, show it is high using the sugar content of C31, it is in good taste.
(4) cucumber booth that Tang of the town villages Bei Liu allows is drawn in Xi'an City, Shanxi Province Changan District, only sprays 1 C31, more daily per canopy
70~80 jin of cucumber are received, and yellow type is straight, taste and sweet mouthfeel, there is the distinctive fragrance of cucumber.Connect spray 3 times after the transplanting of its tomato greenhouse,
Every 5-7 days, tomato growth speed was fast, the strong dress of plant, special mouthfeel are good, and flavor is dense.Its Strawberry Seedlings greenhouse spray 3 times 1000 times
C31, robust seedling stem thickness, Ye great, thickness and black, the Strawberry Seedlings that neighbour family applies 3 chemical fertilizer are obviously sturdy, play the work of weight-reducing synergy
With.Connect spray 5 times after the Luan Zhen Zhang little Jun strawberries transplanting of Changan District, strawberry hardness is good, and flavor is dense, sells lover.
(5) 250 times of C31 of watermelon of the Xianyang, Shanxi province city Chunhua County profit town villages Qin He Lee's magnificence spray 3 times, climing thick and long, leaf
Very, it is more and big (such as fist or egg are big) to sit melon, control is just bloomed substantially, and only a special talent starts to sit melon.Its nectarine is sprayed 3 times,
Blade is wide, thick, and color is black glossy, and peach is big, and young sprout stops long early, is conducive to nutrient and is conveyed to fruit, and does not influence the coming year and bear fruit.
(6) the cold canopy winter jujube that the brilliant town Wang Yan Wang Cun kings in Shaanxi Province's Weinan City Dali County face south
1000 times of foliage-sprays, spray 2 times, sprays 4 times after spending before spending, and performance is that colored head is big, and flower amount is more, and winter jujube listing is early,
Thin skin, crisp, sweet tea are in good taste.It is than control every time to adopt 2 cages more.Key is that this year (2017) tree vigo(u)r restores, and next year, yield did not had to
Worry.
(7) town is drawn in Xi'an City, Shanxi Province Changan District
Asparagus growth period, after 1000 times connect 3 C31 of spray, increment was big, and yield is high, and quality is good, fiber at interval of 5 days
It is few, it is crisp.
Okra growth period, after 1000 times connect 3 C31 of spray, robust plant, the speed of growth was fast, solid more at interval of 5 days.
It these are only the preferred embodiment of the present invention, be not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.Any modification made by all within the spirits and principles of the present invention,
Equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of fruits and vegetables colour upgrading growth promoting liquor, which is characterized in that prepare the fruits and vegetables coloring upgrading growth promoting liquor raw material and its
Mass percent includes:Oligopeptide 1%~5%, Potassium Chitosan Phosphate 0.6%~3%, edible ethanol 1.5%~3%, food
With acetic acid 0.6%~2%, surplus is pure water;Wherein, the Potassium Chitosan Phosphate is molecular weight 200Dal~5000Dal
Oligosaccharide derivatives and the Dal of 100,000 Dal~300,000 poly sugar derivatives composition;The fruits and vegetables colour upgrading growth promoting liquor
Preparation method, include the following steps:
The oligopeptide of the mass percent and pure water are mixed processing 30min~60min, then added again by step 1
Processing 30min is mixed in the edible ethanol for entering the mass percent, obtains oligopeptide solution;
The Potassium Chitosan Phosphate that the molecular weight of the mass percent is 200Dal~5000Dal is put into step by step 2
In rapid one obtained oligopeptide solution, processing 30min is mixed, obtains the oligomeric of the Potassium Chitosan Phosphate of low molecular weight section
Peptide solution;
The Potassium Chitosan Phosphate that the molecular weight of the mass percent is the Dal of 100,000 Dal~300,000 is put into step by step 3
The oligopeptide solution of the Potassium Chitosan Phosphate of rapid two obtained low molecular weight sections, feeds intake, after feeding intake, then mixes while stirring
Close stir process 30min;Obtain the oligopeptide solution of the Potassium Chitosan Phosphate of combined molecular weight section;
Step 4, in the case of stirring, the oligopeptide of the Potassium Chitosan Phosphate of the combined molecular weight section described in step poly- three
In solution, the edible acetic acid of the mass percent is added, continues to stir 60min~90min to get fruits and vegetables coloring upgrading
Growth promoting liquor.
2. fruits and vegetables according to claim 1 colour upgrading growth promoting liquor, which is characterized in that prepare the fruits and vegetables coloring upgrading and promote
The raw material and its mass percent of long liquid include:Oligopeptide 2%, Potassium Chitosan Phosphate 2%, 95% edible ethanol 2.5%,
99% edible acetic acid 1.5%, surplus are pure water.
3. fruits and vegetables according to claim 1 colour upgrading growth promoting liquor, which is characterized in that the oligopeptide is that molecular weight exists
The soybean oligopeptide of 180Dal~1000Dal.
4. fruits and vegetables according to claim 1 colour upgrading growth promoting liquor, which is characterized in that the Potassium Chitosan Phosphate is:
To avenge the back skin of crab as raw material, using chitosan derivatives made of conventional method.
5. fruits and vegetables according to claim 1 colour upgrading growth promoting liquor, which is characterized in that the matter of the oligosaccharide and polysaccharide
Amount is than being 1~3:7~9.
6. fruits and vegetables according to claim 1 colour upgrading growth promoting liquor, which is characterized in that the volume fraction of the edible ethanol
It is 90%~95%.
7. fruits and vegetables according to claim 1 colour upgrading growth promoting liquor, which is characterized in that the edible acetic acid volume fraction is
90%~99%.
8. fruits and vegetables according to claim 1 colour upgrading growth promoting liquor, which is characterized in that the fruits and vegetables colour upgrading growth promoting liquor
Method of administration be:The fruit tree growth phase applies 3 times~6 times, is spaced 15 days~20 days;Vegetables, 2 times~3 times melon, interval 5 days
~7 days.
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102057906A (en) * | 2010-12-10 | 2011-05-18 | 常大勇 | Fruit coloring adjunct and method for promoting fruit coloring with same |
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CN102057906A (en) * | 2010-12-10 | 2011-05-18 | 常大勇 | Fruit coloring adjunct and method for promoting fruit coloring with same |
CN102925524A (en) * | 2012-11-16 | 2013-02-13 | 广州优锐生物科技有限公司 | Seafood leftover oligopeptide and preparation method thereof |
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