CN108315212B - 384-hole plate primer transfer device - Google Patents
384-hole plate primer transfer device Download PDFInfo
- Publication number
- CN108315212B CN108315212B CN201810283941.9A CN201810283941A CN108315212B CN 108315212 B CN108315212 B CN 108315212B CN 201810283941 A CN201810283941 A CN 201810283941A CN 108315212 B CN108315212 B CN 108315212B
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- CN
- China
- Prior art keywords
- hole
- platform
- hole plate
- plate
- yaban
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Links
- 238000012546 transfer Methods 0.000 title claims abstract description 15
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 12
- 229910052786 argon Inorganic materials 0.000 claims description 6
- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 238000012856 packing Methods 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000003028 elevating effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
The invention discloses a 384-hole plate primer transferring device which comprises an upper platform, a lower platform and a lifting platform, wherein the lifting platform comprises a 384-hole plate supporting platform and two 96-hole plate supporting platforms, the upper end of the lower platform is provided with a lifting mechanism, the upper end of the lifting mechanism is fixedly connected with the 384-hole plate supporting platform, the upper end of the lower platform is fixedly provided with a sliding rod, the upper end of the sliding rod penetrates through the lifting platform to be fixedly connected with the upper platform, the upper end of the 384-hole plate supporting platform is provided with a 384-hole plate, a 384-hole pressing plate is arranged above the 384-hole plate, each 96-hole plate supporting platform is provided with two 96-hole plates, 96 Kong Yaban, 384 Kong Yaban and 96 Kong Yaban are correspondingly arranged above each 96-hole plate supporting platform, the upper end of each 96-hole pressing plate is provided with 96-hole first pipe connectors, and 384-hole pressing plates are respectively connected with the first pipe connectors through hoses. The 384-hole plate primer transfer device has simple structure and strong practicability, and is suitable for large-scale popularization.
Description
Technical Field
The invention relates to a primer transfer device, in particular to a 384-well plate primer transfer device.
Background
With the development of biology and the increasing demand for low cost and high throughput of artificially synthesized DNA, large synthetic biology companies in China are now tending to synthesize oligonucleotide chains with a synthesizer with a throughput of 768 for PCR amplification splice assembly into long chain genes. Taking the dr.oligo768 synthesizer imported from the united states as an example, the synthesis throughput is 768 oligonucleotide chains and the final ammonolyzed oligonucleotide chains are in 2 384 well plates. In addition, 384-well PCR apparatus were also 384-well plates, which were all the same size. Because each microwell on 384-well plates is small in size and not suitable for subsequent production experiments, products in 384-well plates are generally packaged in 4 96-well plates and then used for subsequent processing, such as packaging primers in 384-reaction plates in 4 96-well plates and then sequencing by a previous generation sequencer during actual production and test. The common practice of packing the samples in 384 well plates into 4 96 well plates is to use manual sampling, because the size of the micro-holes in 384 well plates is small and the accurate manual packing is very prone to error, although the packing is also performed by using a pipetting workstation, the cost is very high, taking a swiss Tecan pipetting workstation as an example and the standard price of a pipetting workstation is 120 ten thousand RMB, and about 60 minutes are required for packing a 384 well plate into 4 96 well plates. Adding a lot of equipment cost to production and experiment and taking a long time.
Disclosure of Invention
The invention aims to provide a 384-hole plate primer transfer device which has simple structure and strong practicability and is suitable for large-scale popularization.
In order to achieve the above purpose, the present invention provides the following technical solutions:
384 orifice plate primer transfer device, including upper platform, lower platform, lift platform sets up between upper platform and lower platform, lift platform includes 384 orifice plate saddle and two 96 orifice plate saddle, the lower platform upper end is equipped with elevating system, elevating system upper end and 384 orifice plate saddle fixed connection, the lower platform upper end is fixed with the slide bar, the slide bar upper end passes lift platform and upper platform fixed connection, lift platform can reciprocate on the slide bar, 384 orifice plate saddle upper end is equipped with 384 orifice plates, 384 orifice plate top is equipped with 384 Kong Yaban, all be equipped with two 96 orifice plates on every 96 orifice plate saddle, every 96 orifice plate top all corresponds and is equipped with 96 Kong Yaban, 384 Kong Yaban and 96 Kong Yaban all install on the upper platform, every 96 orifice plate upper end all is equipped with 96 coupling one, 384 orifice plate upper end is equipped with 384 coupling two, coupling one and coupling two pass through hose connection respectively.
Preferably, the 384-well plate pallet and the two 96-well plate pallets are of an integrally formed structure.
Preferably, the number of the sliding rods is 4 or more.
Preferably, an argon inlet is formed in the upper end of the 384-hole pressing plate.
Preferably, the two 96-well plate pallets are symmetrically arranged with respect to the 384-well plate pallet.
Preferably, the upper end of the 384-well plate supporting table is provided with a groove for fixing the 384-well plate, and the two 96-well plate supporting tables are respectively provided with a groove for fixing the 96-well plate.
Compared with the prior art, the invention has the beneficial effects that:
the invention has simple structure, convenient operation and extremely low cost, can split the primer in a 384-pore plate into 4 96-pore plates in about 3 minutes, has simple structure and strong practicability, and is suitable for large-scale popularization.
Drawings
FIG. 1 is a front view of a 384-well plate primer transfer device in an embodiment of the present invention;
FIG. 2 is a top view of a 384 well plate primer transfer device in an embodiment of the present invention;
FIG. 3 is a side view of a 384 well plate primer transfer device in an embodiment of the present invention;
in the figure, 1, an upper platform, 2, a lower platform, 3, 384-hole plate pallets, 4, 96-hole plate pallets, 5, a lifting mechanism, 6, a sliding rod, 7, 384-hole plates, 8, 384 Kong Yaban, 9, 96-hole plates, 10, 96 Kong Yaban, 11, a first pipe joint, 12, a second pipe joint, 13, a hose, 14 and an argon inlet.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to FIGS. 1-3, a 384-well plate primer transfer device comprises an upper platform 1, a lower platform 2 and a lifting platform, wherein the lifting platform is arranged between the upper platform 1 and the lower platform 2, the lifting platform comprises a 384-well plate supporting platform 3 and two 96-well plate supporting platforms 4, the 384-well plate supporting platform 3 and the two 96-well plate supporting platforms 4 are of an integrated structure, the two 96-well plate supporting platforms 4 are symmetrically arranged about the 384-well plate supporting platform 3, the upper end of the lower platform 2 is provided with a lifting mechanism 5, the lifting mechanism 5 can be an air cylinder, the upper end of the lifting mechanism 5 is fixedly connected with the 384-well plate supporting platform 3, the upper end of the lower platform 2 is fixedly connected with slide bars 6, the number of the slide bars 6 is 4 or more, the upper end of the slide bars 6 passes through the lifting platform to be fixedly connected with the upper platform 1, the lifting platform can move up and down on the slide bars 6, the upper end of a 384-hole plate supporting table is provided with a groove for fixing a 384-hole plate, the 384-hole plate is fixed in the corresponding groove at the upper end of the 384-hole plate supporting table, 384 Kong Yaban is arranged above the 384-hole plate 7, each 96-hole plate supporting table 4 is provided with two 96-hole plates 9, each 96-hole plate supporting table is provided with a groove for fixing a 96-hole plate, each 96-hole plate 9 is correspondingly provided with 96 Kong Yaban 10, 384 Kong Yaban 8 and 96 Kong Yaban 10 which are respectively arranged on the upper platform 1, each 96-hole pressing plate 10 is provided with 96 first pipe joints 11 at the upper end, 384 second pipe joints 12 are arranged at the upper end of the 384-hole pressing plate 8, the first pipe joints 11 and the second pipe joints 12 are respectively connected through hoses 13, and argon inlets 14 are arranged at the upper end of the 384-hole pressing plate 8.
Working principle: firstly, fixing a 384-hole plate 8 filled with a sample in a corresponding groove on a 384-hole plate supporting table 3, fixing 4 empty 96-hole plates 9 in a corresponding groove on the 96-hole plate supporting table 4, and then lifting the lifting platform by a lifting mechanism 5 to enable the upper surface of the 384-hole plate 7 to be tightly pressed and sealed with the lower surface of a 384-hole pressing plate 8, wherein the 96-hole plates 9 and 96 Kong Yaban are closed but not sealed; then argon is introduced into 384 Kong Yaban from an argon inlet 14, air in 384-well plates 7 and 384-well Kong Yaban is discharged from a gap between the 96-well plate 9 and the 96-well pressing plate 10 through a hose 13, and samples in the 384-well plates 7 are split-packed into 4 corresponding 96-well plates 9; after the split charging, the lifting mechanism 5 is lowered to the lowest position to manually take out the empty 384-well plate 7 and 4 96-well plates 9 filled with samples.
The foregoing is merely illustrative and explanatory of the invention, as it is well within the scope of the invention, as it is intended to provide those skilled in the art with various modifications, additions and substitutions to the specific embodiments disclosed and those skilled in the art without departing from the scope of the invention as disclosed in the accompanying claims.
Claims (4)
1.384 well plate primer transfer device, its characterized in that: the lifting platform is arranged between the upper platform and the lower platform, the lifting platform comprises 384-hole plate pallets and two 96-hole plate pallets, the upper end of the lower platform is provided with a lifting mechanism, the upper end of the lifting mechanism is fixedly connected with the 384-hole plate pallets, the upper end of the lower platform is fixedly provided with a sliding rod, the upper end of the sliding rod penetrates through the lifting platform to be fixedly connected with the upper platform, the lifting platform can move up and down on the sliding rod, the upper end of the 384-hole plate pallet is provided with 384-hole plates, 384-Kong Yaban are arranged above the 384-hole plates, each 96-hole plate pallet is provided with two 96-hole plates, 96-Kong Yaban are correspondingly arranged above each 96-hole plate pallet, 384-hole plates Kong Yaban and 96-Kong Yaban are respectively arranged on the upper platform, each 96-hole plate pallet is provided with 96-hole pipe joints I, and 384-hole plate pressing plates are respectively connected with 384-hole pipe joints II through hoses;
the number of the sliding rods is 4 or more than 4;
an argon inlet is formed in the upper end of the 384-hole pressing plate.
2. The 384-well plate primer transfer device of claim 1, wherein: the 384-hole plate supporting table and the two 96-hole plate supporting tables are of an integrated structure.
3. The 384-well plate primer transfer device of claim 1, wherein: the two 96-well plate pallets are symmetrically arranged with respect to the 384-well plate pallet.
4. The 384-well plate primer transfer device of claim 1, wherein: the upper end of the 384-hole plate supporting table is provided with a groove for fixing the 384-hole plate, and the two 96-hole plate supporting tables are respectively provided with a groove for fixing the 96-hole plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810283941.9A CN108315212B (en) | 2018-04-02 | 2018-04-02 | 384-hole plate primer transfer device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810283941.9A CN108315212B (en) | 2018-04-02 | 2018-04-02 | 384-hole plate primer transfer device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108315212A CN108315212A (en) | 2018-07-24 |
| CN108315212B true CN108315212B (en) | 2023-11-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810283941.9A Active CN108315212B (en) | 2018-04-02 | 2018-04-02 | 384-hole plate primer transfer device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108315212B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111018931A (en) * | 2019-12-30 | 2020-04-17 | 江苏东玄基因科技有限公司 | Automatic solid-phase synthesis system for oligomer |
Citations (7)
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|---|---|---|---|---|
| CN2721223Y (en) * | 2004-09-03 | 2005-08-31 | 北京博奥生物芯片有限责任公司 | Multi-channel liquid transfer device |
| CN101680873A (en) * | 2007-04-04 | 2010-03-24 | 加利福尼亚大学董事会 | Use composition, equipment, the system and method for nano-pore |
| JP2011006902A (en) * | 2009-06-25 | 2011-01-13 | Hitachi Constr Mach Co Ltd | Construction machinery |
| CN103923163A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Workbench system and the method for bioactive substance extractor |
| CN104263633A (en) * | 2014-09-24 | 2015-01-07 | 东南大学 | Magnetic-separation-based high-flux biological sample treatment device |
| CN105188936A (en) * | 2013-01-15 | 2015-12-23 | 瑞宁器材公司 | Liquid end assembly for a multichannel air displacement pipette |
| CN208266157U (en) * | 2018-04-02 | 2018-12-21 | 通用生物系统（安徽）有限公司 | 384 orifice plate primer transfer devices |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030161761A1 (en) * | 2002-02-28 | 2003-08-28 | Williams Roger O. | Apparatus and method for composing high density materials onto target substrates by a rapid sequence |
-
2018
- 2018-04-02 CN CN201810283941.9A patent/CN108315212B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2721223Y (en) * | 2004-09-03 | 2005-08-31 | 北京博奥生物芯片有限责任公司 | Multi-channel liquid transfer device |
| CN101680873A (en) * | 2007-04-04 | 2010-03-24 | 加利福尼亚大学董事会 | Use composition, equipment, the system and method for nano-pore |
| JP2011006902A (en) * | 2009-06-25 | 2011-01-13 | Hitachi Constr Mach Co Ltd | Construction machinery |
| CN103923163A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Workbench system and the method for bioactive substance extractor |
| CN105188936A (en) * | 2013-01-15 | 2015-12-23 | 瑞宁器材公司 | Liquid end assembly for a multichannel air displacement pipette |
| CN104263633A (en) * | 2014-09-24 | 2015-01-07 | 东南大学 | Magnetic-separation-based high-flux biological sample treatment device |
| CN208266157U (en) * | 2018-04-02 | 2018-12-21 | 通用生物系统（安徽）有限公司 | 384 orifice plate primer transfer devices |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108315212A (en) | 2018-07-24 |
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Address after: 239000 No.69, qifushi West Road, Chuzhou economic and Technological Development Zone, Anhui Province Applicant after: General Biology (Anhui) Co.,Ltd. Address before: 239000 No.6 yongyang Road, Chuzhou economic and Technological Development Zone, Anhui Province Applicant before: GENERAL BIOSYSTEMS (ANHUI), Inc. |
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