CN108309956A - A kind of tunica fibrosa and preparation method thereof for local anaesthesia - Google Patents
A kind of tunica fibrosa and preparation method thereof for local anaesthesia Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
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- A—HUMAN NECESSITIES
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- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
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Abstract
The present invention relates to medical anesthesia technical fields, more particularly to a kind of tunica fibrosa and preparation method thereof for local anaesthesia, the tunica fibrosa includes heat conduction surface layer and function and service layer, the heat conduction surface layer is carboxylated modified graphene layer, the function and service layer is the fiber membrane that poly- N caprolactams and ethyl cellulose mixing electrostatic spinning are formed, and the anther sac particle for being useful for anesthesia is loaded on the fiber membrane.The tunica fibrosa of the present invention is the tunica fibrosa that carboxylated modified graphene spinning solution, composite spinning liquid are successively carried out to electrostatic spinning formation.The tunica fibrosa of the present invention can effectively keep the anesthesia drug effect of herbal anesthetic, avoid its inactivation, anaesthetic effect can quickly and efficiently be reached by acting on local anaesthesia skin surface, it is directly attached to local anaesthesia skin surface when use, can avoid the problem that smearing anesthetic cream pollution bedding, clothing.
Description
Technical field
The present invention relates to medical anesthesia technical field more particularly to a kind of tunica fibrosas and its preparation side for local anaesthesia
Method.
Background technology
Even if anesthesia makes entire patient or part temporarily and reversibly loses reaction, to reach with drug or other methods
The purpose that can be smoothed out to operation.Related anaesthesia technology, in the world earliest literature record occur from the Han dynasty in China,
The fiber crops boiling soup invented by the famous surgical specialist of ancient Chinese, traditional Chinese medicine scholar Huatuo.Fiber crops boiling soup is that traditional Chinese herbal decoction is equivalent to now
Oral general anesthesia agent, after taking orally patient dermal sensation lose and operation convenient to carry out.Later doctor trained in Western medicine has invented various anesthetic, such as
Present lidocaine prevailing, Bupivacaine, procaine etc..In recent years, it has been found that the secondary of various Western medicine anesthetic makees
With larger, many senses of discomfort can be brought to patient, then various herbal anesthetics occur again after anesthesia.In but
Medicine anesthetic is mostly the plant extract agent for having effects that narcotic analgesic, and activity is not easy to keep, and anaesthetic effect reduces.
Due to the development of modern medicine and related discipline, range of operation constantly expands, and many original operation forbidden zones are
It can successfully perform the operation, these are all that development with the department of anaesthesiology is undivided.Local epidermis anesthesia is Minimally Invasive Surgery
One of with the common auxiliary treatment means such as beauty treatment, mostly with oral analgesic, local injection anesthetic and anesthesia breast is smeared
Herbal paste formula is anaesthetized.Oral pain alleviating drug effect unobvious, and curative effect is short；Local injection local anaesthetics infiltration anesthesia is in injection
Apparent feeling of pain is will produce, patient anxiety may be allowed uneasy, and injects excessive local anaesthetics to generate one to therapeutic effect
Fixed harmful effect；Anesthetic cream is smeared, although not will produce feeling of pain, the anesthetic composition in emulsifiable paste is not easy to penetrate into
Skin histology, it is also necessary to smear certain thickness and can be only achieved anaesthetic effect, practical operation can not accurately control applying amount, and be easy
It is attached to bedding, on clothing, pollution bedding, clothing.Therefore, study a kind of activity-maintaining time is long, anaesthetic can be quick
Infiltration immerses subcutaneous tissue and reaches anaesthetic effect, and can directly be attached to the tunica fibrosa of local anaesthesia skin surface with important
Meaning.
Invention content
In view of this, the tunica fibrosa and preparation method thereof that the object of the present invention is to provide a kind of for local anaesthesia, the fibre
Dimension film can effectively keep the anesthesia drug effect of herbal anesthetic, avoid its inactivation, acting on local anaesthesia skin surface can be fast
Speed effectively achieves anaesthetic effect, and when use is directly attached to local anaesthesia skin surface, can avoid smearing anesthetic cream dirty
The problem of contaminating bedding, clothing.
The present invention solves above-mentioned technical problem by following technological means：
A kind of tunica fibrosa for local anaesthesia, including heat conduction surface layer and function and service layer, the heat conduction surface layer are carboxyl
Change modified graphene layer, the function and service layer is that poly-N-vinylcaprolactam and ethyl cellulose mixing electrostatic spinning are formed
Fiber membrane, on the fiber membrane load be useful for the anther sac particle of anesthesia.
Poly-N-vinylcaprolactam is a kind of responsive to temperature type intelligent macromolecule material, has good ionic water-soluble
Property and organic solvent solubility, the absorbability of good biocompatibility and superelevation, phase transition temperature be 33 DEG C in human body
In Physiological temperatures range；Ethyl cellulose is a kind of cellulose derivative, and physicochemical properties are stablized, and have good biofacies
Capacitive.The present invention tunica fibrosa when in use in combination with illumination so as to accelerate anaesthetize drug effect osmotic absorption, illumination local anaesthesia
Position can increase the surface temperature at local anaesthesia position so that poly-N-vinylcaprolactam is undergone phase transition, and release anther sac is micro-
The anesthesia drug effect of grain promotes absorption of the local anaesthesia position to arcotic, so achieve the effect that it is quick, effectively anaesthetize；Graphite
Alkene has stronger thermal conductivity, and carboxylated modified graphene layer is made heat conduction surface layer, is conducive to heat under the conditions of illumination when use
Conduction, to achieve the purpose that local anaesthesia portion faces are rapidly heated.By for the anther sac doped particles of anesthesia in fiber
Film not only can effectively keep the drug effect of anther sac particle for a long time, but also can be to avoid anesthetic cream class contamination of products bedding, clothing etc.
Problem.
Further, the mass ratio of the poly-N-vinylcaprolactam in the fiber membrane and ethyl cellulose be 1.0~
1.3:1。
Such ratio setting not only improves fiber membrane and is undergone phase transition under the conditions of certain temperature, discharges drug effect, and have
It is maintained conducive to the drug effect of anther sac particle under storage requirement.
Further, the anther sac particle for having 20%~25% is loaded on the fiber membrane.
Further, contain 10%~15% herbal anesthetic in the anther sac particle.
Further, the herbal anesthetic include from datura flower, arabian jasmine root, monkshood, Rhododendron molle, girald daphne bark one kind or
The extractant extracted in various plants further includes peppermint oil and sesame oil.
Datura flower, arabian jasmine root, monkshood, Rhododendron molle, girald daphne bark all have narcotic analgesic effect, safe using Chinese medicine preparation,
Small side effects.Using peppermint oil and sesame oil as skin bleeding agent is promoted, fast and effectively permeated further such that arcotic efficiency is enough
Skin histology acts on.
Further, the herbal anesthetic is coated with lecithin modification chitosan, in the lecithin modification chitosan
The deacetylation degree of chitosan is 75%~90%.
Chitosan from the natural alkaline mucopolysaccharide of animal there is no biotoxicity and good readily degradable can wait
Advantage, while also having the function of antibacterial anti-inflammatory, the chitosan through lecithin modification can store for embedding herbal anesthetic
It plays a protective role to herbal anesthetic when depositing, and lecithin is stronger in the osmotic effect of keratoderma, can further strengthen
Osmosis of the herbal anesthetic at local anaesthesia position.
In addition, the invention also discloses a kind of preparation method of above-mentioned tunica fibrosa for local anaesthesia, including it is following
Step：
The preparation of carboxylated modified graphene：Take graphene that the sodium hydroxide solution of a concentration of 0.2~0.4mol/L is added
Middle ultrasonic wave disperses 3h, and 2.5~3.5h of monoxone ultrasonic response is then added and obtains reaction solution, by reaction solution deionized water
Washing is centrifuged repeatedly to neutrality, obtains carboxylated modified graphene spinning solution；
The preparation of local anaesthesia tunica fibrosa：It takes poly-N-vinylcaprolactam and ethyl cellulose that mass concentration is added to be
It is stirred and evenly mixed in 50% ethanol solution, anther sac particle is then added and stirs to being uniformly dispersed, obtains composite spinning liquid；By carboxyl
Change modified graphene spinning solution in 13~15kV of voltage, 30~35 DEG C of temperature, humidity 45%~55%, flow velocity 0.5mL/h conditions
Lower electrostatic spinning forms modified graphene film in injection to film die, then by composite spinning liquid in 13~15kV of voltage, temperature
Electrostatic spinning under the conditions of 20~25 DEG C of degree, humidity 45%~55%, flow velocity 0.5mL/h, injection to modified graphene film surface,
The dry fiber membrane that is formed by curing for 24 hours is to get to local anaesthesia tunica fibrosa.Lecithin modification chitosan is positively charged, and carboxyl
Change modified graphene layer it is negatively charged, composite spinning liquid is sprayed to modified graphene film surface and is formed by curing film, by just,
The adelphotaxy of negative electrical charge enhances the cohesive force on heat conduction surface layer and function and service layer.
Further, the anther sac particle prepare it is as follows：Take lecithin modification chitosan that ultrasonic wavelength-division in distilled water is added
It dissipates, adds herbal anesthetic and stir to mixing, freeze-drying obtains anther sac particle.
Further, the herbal anesthetic prepare it is as follows：It takes in datura flower, arabian jasmine root, monkshood, Rhododendron molle, girald daphne bark
It is one or more clean, be dry, being ground to and sieve with 100 mesh sieve, Plant Powder is obtained, then with the silk filter cloth of 200 mesh by plant
Powder is packaged into the pack that specification is 5g/ packets, in diaphragm electrolytic cell, in the condition of the sodium chloride solution of 2w%, 350V voltages
Lower energization is electrolysed 45~55 μ s, 30~40min of soak at room temperature, then takes out pack and is placed in vacuum centrifuge, in rotating speed
750rpm 20~25min of traditional vacuum place into freeze drier after drying, keep 3h under -10~-5 DEG C of environment, then
Supercritical carbon dioxide extracting is carried out, anesthesia extractant is obtained, is 2 by mass ratio:1:1 sesame oil, anaesthetizes extractant at peppermint oil
It is uniformly mixed, is concentrated to give herbal anesthetic.
Fresh place is locked using electrolysis activation processing, the processing of centrifugation bleed, freeze-drying process, freezing successively to anesthesia plant
Reason, supercritical extract processing so that the anesthesia extractant activity extracted is good, it is easier to act on human body, have and take effect
Fast feature can be activated preferably and retain anesthetic composition.
Further, the preparation of the lecithin modification chitosan：Chitosan is taken to be added to the acetic acid that mass fraction is 1% molten
In liquid, 90 DEG C are heated to, the lecithin of 1~1.5 times of chitosan mass, ultrasonic emulsification 10min, heat preservation is then added
0.5~1h, in 50~60 DEG C of vacuum drying, grinding obtains lecithin modification chitosan.
The tunica fibrosa for local anaesthesia of the present invention is in use, directly by tunica fibrosa fitting and local anaesthesia skin table
Face increases site of anesthesia surface temperature, the poly-N-vinylcaprolactam in fiber membrane is made to occur in combination with daylight light irradiation
Phase transformation releases to will anaesthetize drug effect, achieve the effect that it is quick, effectively anaesthetize.
Specific implementation mode
Below with reference to specific embodiment, the present invention is described in detail：
A kind of tunica fibrosa for local anaesthesia of the present invention, including heat conduction surface layer and function and service layer, wherein heat conduction table
Layer is carboxylated modified graphene layer；Function and service layer is poly-N-vinylcaprolactam and ethyl cellulose mixing electrostatic spinning
The mass ratio of the fiber membrane of formation, poly-N-vinylcaprolactam and ethyl cellulose in fiber membrane is 1.0~1.3:
1, on fiber membrane load have 20%~25% anther sac particle for anesthesia, containing in 10%~15% in anther sac particle
Medicine anesthetic, herbal anesthetic include from one or more plants in datura flower, arabian jasmine root, monkshood, Rhododendron molle, girald daphne bark
The extractant of extraction, further includes peppermint oil and sesame oil, and herbal anesthetic is coated with lecithin modification chitosan, lecithin modification
The deacetylation degree of chitosan is 75%~90% in chitosan.
Preparing for the tunica fibrosa for local anaesthesia of the present invention is as follows：
The preparation of carboxylated modified graphene：Take graphene that the sodium hydroxide solution of a concentration of 0.2~0.4mol/L is added
Middle ultrasonic wave disperses 3h, and 2.5~3.5h of monoxone ultrasonic response is then added and obtains reaction solution, by reaction solution deionized water
Washing is centrifuged repeatedly to neutrality, obtains carboxylated modified graphene spinning solution；
The preparation of local anaesthesia tunica fibrosa：It takes poly-N-vinylcaprolactam and ethyl cellulose that mass concentration is added to be
It is stirred and evenly mixed in 50% ethanol solution, anther sac particle is then added and stirs to being uniformly dispersed, obtains composite spinning liquid；By carboxyl
Change modified graphene spinning solution in 13~15kV of voltage, 30~35 DEG C of temperature, humidity 45%~55%, flow velocity 0.5mL/h conditions
Lower electrostatic spinning forms modified graphene film in injection to film die, then by composite spinning liquid in 13~15kV of voltage, temperature
Electrostatic spinning under the conditions of 20~25 DEG C of degree, humidity 45%~55%, flow velocity 0.5mL/h, injection to modified graphene film surface,
The dry fiber membrane that is formed by curing for 24 hours is to get to local anaesthesia tunica fibrosa.
The preparation method of the tunica fibrosa for local anaesthesia of the present invention will be illustrated below, it is as follows：
Embodiment one
The preparation of lecithin modification chitosan：It is that 75% chitosan is added to 500mL mass point to take 5g deacetylation degree
5g lecithin is then added in 1% acetic acid solution, to be heated to 90 DEG C in number, and ultrasonic emulsification 10min keeps the temperature 0.5h,
In 50~60 DEG C of vacuum drying, grinding obtains lecithin modification chitosan.
The preparation of herbal anesthetic：It takes datura flower, arabian jasmine root, monkshood, Rhododendron molle, girald daphne bark to be cleaned up with clear water, subtracts
It press dry dry, is ground to and sieves with 100 mesh sieve, obtain Plant Powder, Plant Powder, which is then packaged into specification, with the silk filter cloth of 200 mesh is
The pack of 5g/ packets, is put into diaphragm electrolytic cell, in mass concentration be 2% sodium chloride solution, lead under conditions of 350V voltages
Electricity electrolysis 55 μ s, soak at room temperature 30min then take out pack and are placed in vacuum centrifuge, in rotating speed 750rpm traditional vacuums
20min is placed into freeze drier after drying, 3h is kept under -10~-5 DEG C of environment, then carries out supercritical carbon dioxide
Extraction obtains anesthesia extractant, is 2 by mass ratio:1:1 sesame oil, peppermint oil, anesthesia extractant are uniformly mixed, and are concentrated to give
Herbal anesthetic.
The preparation of anther sac particle：It takes 2g lecithin modification chitosans that ultrasonic wave in 100mL distilled waters is added to disperse, add
0.2g herbal anesthetics are stirred to mixing, and freeze-drying obtains anther sac particle.
The preparation of carboxylated modified graphene：Graphene is taken to be added in the sodium hydroxide solution of a concentration of 0.2mol/L ultrasonic
Wavelength-division dissipates 3h, and excessive monoxone ultrasonic response 2.5h is then added and obtains reaction solution, repeatedly with deionized water by reaction solution
Centrifuge washing obtains carboxylated modified graphene spinning solution to neutrality.
The preparation of local anaesthesia tunica fibrosa：Take 2.0g poly-N-vinylcaprolactams and 2g ethyl celluloses that 60mL matter is added
It measures and is stirred and evenly mixed in a concentration of 50% ethanol solution, 0.8g anther sac particles are then added and stir to being uniformly dispersed, obtain compound
Spinning solution；By carboxylated modified graphene spinning solution under the conditions of voltage 13kV, 30 DEG C of temperature, humidity 45%, flow velocity 0.5mL/h
Carry out electrostatic spinning, syringe needle is kept during electrostatic spinning and receive reception 18~20cm of distance of die surface, injection at
Modified graphene film is formed in film die, then by composite spinning liquid in voltage 13,20 DEG C of temperature, humidity 45%, flow velocity
Electrostatic spinning under the conditions of 0.5mL/h, electrostatic spinning keep syringe needle and receive reception 18~20cm of distance of die surface in the process,
Injection is to modified graphene film surface, and the dry fiber membrane that is formed by curing for 24 hours is to get to local anaesthesia tunica fibrosa.
Embodiment two
The preparation of lecithin modification chitosan：It is that 80% chitosan is added to 500mL mass point to take 5g deacetylation degree
6g lecithin is then added in 1% acetic acid solution, to be heated to 90 DEG C in number, and ultrasonic emulsification 10min keeps the temperature 0.6h,
In 50~60 DEG C of vacuum drying, grinding obtains lecithin modification chitosan.
The preparation of herbal anesthetic：It takes datura flower, arabian jasmine root, monkshood, Rhododendron molle, girald daphne bark to be cleaned up with clear water, subtracts
It press dry dry, is ground to and sieves with 100 mesh sieve, obtain Plant Powder, Plant Powder, which is then packaged into specification, with the silk filter cloth of 200 mesh is
The pack of 5g/ packets, is put into diaphragm electrolytic cell, in mass concentration be 2% sodium chloride solution, lead under conditions of 350V voltages
Electricity electrolysis 50 μ s, soak at room temperature 35min then take out pack and are placed in vacuum centrifuge, in rotating speed 750rpm traditional vacuums
22min is placed into freeze drier after drying, 3h is kept under -10~-5 DEG C of environment, then carries out supercritical carbon dioxide
Extraction obtains anesthesia extractant, is 2 by mass ratio:1:1 sesame oil, peppermint oil, anesthesia extractant are uniformly mixed, and are concentrated to give
Herbal anesthetic.
The preparation of anther sac particle：It takes 2g lecithin modification chitosans that ultrasonic wave in 100mL distilled waters is added to disperse, add
0.24g herbal anesthetics are stirred to mixing, and freeze-drying obtains anther sac particle.
The preparation of carboxylated modified graphene：Graphene is taken to be added in the sodium hydroxide solution of a concentration of 0.3mol/L ultrasonic
Wavelength-division dissipates 3h, and excessive monoxone ultrasonic response 3.0h is then added and obtains reaction solution, repeatedly with deionized water by reaction solution
Centrifuge washing obtains carboxylated modified graphene spinning solution to neutrality.
The preparation of local anaesthesia tunica fibrosa：Take 2.2g poly-N-vinylcaprolactams and 2g ethyl celluloses that 60mL matter is added
It measures and is stirred and evenly mixed in a concentration of 50% ethanol solution, 0.924g anther sac particles are then added and stir to being uniformly dispersed, are answered
Close spinning solution；By carboxylated modified graphene spinning solution in voltage 15kV, 35 DEG C of temperature, humidity 55%, flow velocity 0.5mL/h conditions
Lower carry out electrostatic spinning, electrostatic spinning keep syringe needle and receive reception 18~20cm of distance of die surface in the process, and injection is extremely
Modified graphene film is formed in film die, then by composite spinning liquid in voltage 15kV, 25 DEG C of temperature, humidity 55%, flow velocity
Electrostatic spinning under the conditions of 0.5mL/h, electrostatic spinning keep syringe needle and receive reception 18~20cm of distance of die surface in the process,
Injection is to modified graphene film surface, and the dry fiber membrane that is formed by curing for 24 hours is to get to local anaesthesia tunica fibrosa.
Embodiment three
The preparation of lecithin modification chitosan：It is that 85% chitosan is added to 500mL mass point to take 5g deacetylation degree
7g lecithin is then added in 1% acetic acid solution, to be heated to 90 DEG C in number, and ultrasonic emulsification 10min keeps the temperature 0.8h,
In 50~60 DEG C of vacuum drying, grinding obtains lecithin modification chitosan.
The preparation of herbal anesthetic：It takes girald daphne bark to be cleaned up with clear water, is dried under reduced pressure, be ground to and sieve with 100 mesh sieve, obtain
Plant Powder is then packaged into the pack that specification is 5g/ packets with the silk filter cloth of 200 mesh, is put into diaphragm electrolytic cell by Plant Powder
In, in mass concentration be 2% sodium chloride solution, be powered under conditions of 350V voltages electrolysis 47 μ s, soak at room temperature 35min, with
Pack is taken out afterwards to be placed in vacuum centrifuge, in rotating speed 750rpm traditional vacuum 22min, is placed into dry in freeze drier
Afterwards, 3h is kept under -10~-5 DEG C of environment, then carries out supercritical carbon dioxide extracting, anesthesia extractant is obtained, by mass ratio
It is 2:1:1 sesame oil, peppermint oil, anesthesia extractant are uniformly mixed, and are concentrated to give herbal anesthetic.
The preparation of anther sac particle：It takes 2g lecithin modification chitosans that ultrasonic wave in 100mL distilled waters is added to disperse, add
0.28g herbal anesthetics are stirred to mixing, and freeze-drying obtains anther sac particle.
The preparation of carboxylated modified graphene：Graphene is taken to be added in the sodium hydroxide solution of a concentration of 0.3mol/L ultrasonic
Wavelength-division dissipates 3h, and excessive monoxone ultrasonic response 3.0h is then added and obtains reaction solution, repeatedly with deionized water by reaction solution
Centrifuge washing obtains carboxylated modified graphene spinning solution to neutrality.
The preparation of local anaesthesia tunica fibrosa：Take 2.4g poly-N-vinylcaprolactams and 2g ethyl celluloses that 60mL matter is added
It measures and is stirred and evenly mixed in a concentration of 50% ethanol solution, 1.056g anther sac particles are then added and stir to being uniformly dispersed, are answered
Close spinning solution；By carboxylated modified graphene spinning solution in voltage 14kV, 33 DEG C of temperature, humidity 50%, flow velocity 0.5mL/h conditions
Lower carry out electrostatic spinning, electrostatic spinning keep syringe needle and receive reception 18~20cm of distance of die surface in the process, and injection is extremely
Modified graphene film is formed in film die, then by composite spinning liquid in voltage 14kV, 23 DEG C of temperature, humidity 50%, flow velocity
Electrostatic spinning under the conditions of 0.5mL/h, electrostatic spinning keep syringe needle and receive reception 18~20cm of distance of die surface in the process,
Injection is to modified graphene film surface, and the dry fiber membrane that is formed by curing for 24 hours is to get to local anaesthesia tunica fibrosa.
Example IV
The preparation of lecithin modification chitosan：It is that 90% chitosan is added to 500mL mass point to take 5g deacetylation degree
7.5g lecithin is then added in 1% acetic acid solution, to be heated to 90 DEG C in number, and ultrasonic emulsification 10min keeps the temperature 1h,
In 50~60 DEG C of vacuum drying, grinding obtains lecithin modification chitosan.
The preparation of herbal anesthetic：It takes datura flower, arabian jasmine root, monkshood to be cleaned up with clear water, is dried under reduced pressure, be ground to
100 mesh sieve, and obtain Plant Powder, and Plant Powder is then packaged into the pack that specification is 5g/ packets with the silk filter cloth of 200 mesh, is put into
In diaphragm electrolytic cell, in mass concentration be 2% sodium chloride solution, be powered under conditions of 350V voltages 45 μ s of electrolysis, room temperature
40min is impregnated, pack is then taken out and is placed in vacuum centrifuge, in rotating speed 750rpm traditional vacuum 25min, it is dry to place into freezing
In dry machine after drying, 3h is kept under -10~-5 DEG C of environment, then carries out supercritical carbon dioxide extracting, obtain anesthesia extraction
Mass ratio is 2 by agent:1:1 sesame oil, peppermint oil, anesthesia extractant are uniformly mixed, and are concentrated to give herbal anesthetic.
The preparation of anther sac particle：It takes 2g lecithin modification chitosans that ultrasonic wave in 100mL distilled waters is added to disperse, add
0.30g herbal anesthetics are stirred to mixing, and freeze-drying obtains anther sac particle.
The preparation of carboxylated modified graphene：Graphene is taken to be added in the sodium hydroxide solution of a concentration of 0.4mol/L ultrasonic
Wavelength-division dissipates 3h, and excessive monoxone ultrasonic response 3.5h is then added and obtains reaction solution, repeatedly with deionized water by reaction solution
Centrifuge washing obtains carboxylated modified graphene spinning solution to neutrality.
The preparation of local anaesthesia tunica fibrosa：Take 2.6g poly-N-vinylcaprolactams and 2g ethyl celluloses that 60mL matter is added
It measures and is stirred and evenly mixed in a concentration of 50% ethanol solution, 1.15g anther sac particles are then added and stir to being uniformly dispersed, obtain compound
Spinning solution；By carboxylated modified graphene spinning solution under the conditions of voltage 14kV, 34 DEG C of temperature, humidity 48%, flow velocity 0.5mL/h
Carry out electrostatic spinning, syringe needle is kept during electrostatic spinning and receive reception 18~20cm of distance of die surface, injection at
Modified graphene film is formed in film die, then by composite spinning liquid in voltage 14kV, 24 DEG C of temperature, humidity 48%, flow velocity
Electrostatic spinning under the conditions of 0.5mL/h, electrostatic spinning keep syringe needle and receive reception 18~20cm of distance of die surface in the process,
Injection is to modified graphene film surface, and the dry fiber membrane that is formed by curing for 24 hours is to get to local anaesthesia tunica fibrosa.
The obtained tunica fibrosa for local anaesthesia made above increases anesthesia portion in use, in combination with daylight light irradiation
Position surface temperature, makes the poly-N-vinylcaprolactam in fiber membrane undergo phase transition, and releases, reaches to anaesthetize drug effect
To effect that is quick, effectively anaesthetizing.
The above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although with reference to preferred embodiment to this hair
It is bright to be described in detail, it will be understood by those of ordinary skill in the art that, it can modify to technical scheme of the present invention
Or equivalent replacement should all cover the claim in the present invention without departing from the objective and range of technical solution of the present invention
In range.Technology that the present invention is not described in detail, shape, construction part are known technology.
Claims (10)
1. a kind of tunica fibrosa for local anaesthesia, which is characterized in that including heat conduction surface layer and function and service layer, the heat conduction table
Layer is carboxylated modified graphene layer, and the function and service layer is poly-N-vinylcaprolactam and ethyl cellulose mixing electrostatic
The fiber membrane that spinning is formed, on the fiber membrane load be useful for the anther sac particle of anesthesia.
2. a kind of tunica fibrosa for local anaesthesia according to claim 1, which is characterized in that in the fiber membrane
The mass ratio of poly-N-vinylcaprolactam and ethyl cellulose is 1.0~1.3:1.
3. a kind of tunica fibrosa for local anaesthesia according to claim 2, which is characterized in that born on the fiber membrane
It is loaded with 20%~25% anther sac particle.
4. a kind of tunica fibrosa for local anaesthesia according to claim 3, which is characterized in that contain in the anther sac particle
There is 10%~15% herbal anesthetic.
5. a kind of tunica fibrosa for local anaesthesia according to claim 4, which is characterized in that the herbal anesthetic packet
The extractant extracted from one or more plants in datura flower, arabian jasmine root, monkshood, Rhododendron molle, girald daphne bark is included, further includes thin
Lotus oil and sesame oil.
6. a kind of tunica fibrosa for local anaesthesia according to claim 5, which is characterized in that outside the herbal anesthetic
It is coated with lecithin modification chitosan, the deacetylation degree of chitosan is 75%~90% in the lecithin modification chitosan.
7. according to a kind of preparation method of any tunica fibrosa for local anaesthesia of claim 1~6, feature exists
In including the following steps：
The preparation of carboxylated modified graphene：It takes graphene to be added in the sodium hydroxide solution of a concentration of 0.2~0.4mol/L to surpass
Sound wave disperses 3h, and 2.5~3.5h of monoxone ultrasonic response is then added and obtains reaction solution, repeatedly with deionized water by reaction solution
Centrifuge washing obtains carboxylated modified graphene spinning solution to neutrality；
The preparation of local anaesthesia tunica fibrosa：It is 50% to take poly-N-vinylcaprolactam and ethyl cellulose that mass concentration is added
It is stirred and evenly mixed in ethanol solution, anther sac particle is then added and stirs to being uniformly dispersed, obtains composite spinning liquid；Carboxylated is modified
Graphene spinning solution electrostatic under the conditions of 13~15kV of voltage, 30~35 DEG C of temperature, humidity 45%~55%, flow velocity 0.5mL/h
Spinning, injection to forming modified graphene film in film die, then by composite spinning liquid in 13~15kV of voltage, temperature 20~
25 DEG C, humidity 45%~55%, electrostatic spinning under the conditions of flow velocity 0.5mL/h, injection to modified graphene film surface is dry
It is formed by curing fiber membrane for 24 hours to get to local anaesthesia tunica fibrosa.
8. a kind of preparation method of tunica fibrosa for local anaesthesia according to claim 7, which is characterized in that the medicine
Preparing for capsule particle is as follows：It takes lecithin modification chitosan that ultrasonic wave in distilled water is added to disperse, adds herbal anesthetic and stir
It mixes to mixing, freeze-drying obtains anther sac particle.
9. a kind of preparation method of tunica fibrosa for local anaesthesia according to claim 8, which is characterized in that in described
Preparing for medicine anesthetic is as follows：Take in datura flower, arabian jasmine root, monkshood, Rhododendron molle, girald daphne bark it is one or more clean, be dry,
It is ground to and sieves with 100 mesh sieve, obtain Plant Powder, Plant Powder is then packaged into the medicine that specification is 5g/ packets with the silk filter cloth of 200 mesh
Packet, in diaphragm electrolytic cell, be powered 45~55 μ s of electrolysis under conditions of the sodium chloride solution, 350V voltages of 2w%, room temperature
30~40min is impregnated, pack is then taken out and is placed in vacuum centrifuge, in rotating speed 750rpm 20~25min of traditional vacuum, then put
Enter after being dried in freeze drier, 3h is kept under -10~-5 DEG C of environment, supercritical carbon dioxide extracting is then carried out, obtains
Extractant is anaesthetized, is 2 by mass ratio:1:1 sesame oil, peppermint oil, anesthesia extractant are uniformly mixed, and are concentrated to give Chinese drug anesthesia
Agent.
10. a kind of preparation method of tunica fibrosa for local anaesthesia according to claim 9, which is characterized in that described
Preparing for lecithin modification chitosan is as follows：It takes chitosan to be added in the acetic acid solution that mass fraction is 1%, is heated to
90 DEG C, the lecithin of 1~1.5 times of chitosan mass is then added, ultrasonic emulsification 10min keeps the temperature 0.5~1h, in 50~60
DEG C vacuum drying, grinding obtain lecithin modification chitosan.
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