CN108309287B

CN108309287B - Physical detection method for early screening of brain function narrowing

Info

Publication number: CN108309287B
Application number: CN201810025350.1A
Authority: CN
Inventors: 杨杰
Original assignee: Guangzhou Rainjet Medical Equipment Co ltd
Current assignee: Guangzhou Rainjet Medical Equipment Co ltd
Priority date: 2018-01-09
Filing date: 2018-01-09
Publication date: 2022-06-14
Anticipated expiration: 2038-01-09
Also published as: CN108309287A

Abstract

The invention provides a physical detection method for early screening of brain function narrowing, which comprises the following steps: step S1: stimulating and arranging; adopting a voice material as stimulation, wherein the length of the voice material is 230-300 ms, and the stimulation interval is not less than 700 ms; step S2: collecting data; randomly playing a plurality of stimuli through a loudspeaker, wherein the volume is 80dB, and the playing time is 15 minutes in total; taking the left earlobe and the right earlobe as reference electrodes, and recording continuous electroencephalogram signals by using a brain electrical amplifier (Rainjet); amplifying the EEG signals by an amplifier, extracting the EEG signals in a segmented manner, and recording the EEG signals after correction; step S3: processing data; superposing and averaging the recorded electroencephalogram signals, and respectively calculating the average values of the left and right brain areas to obtain two curves; step S4: waveform identification; searching and identifying the trough of the curve, and automatically extracting the latency and amplitude of the curve. The invention can be used for early screening of the risk of language disorder and autism; acquiring late negative slow waves related to language disorder through voice stimulation evoked event related potential; fills the blank of the early screening of the physical detection of autism at home and abroad.

Description

Physical detection method for early screening of brain function narrowing

Technical Field

The invention relates to a physical detection method for early screening of brain function narrowing.

Background

Pavlov states that the most fundamental activity of the cerebral cortex is signaling activity, essentially distinguishing conditioned stimuli into two broad categories: one is a real specific stimulus, such as sound, light, electricity, taste, etc., called a first signal; the other is an abstract stimulus, i.e. language text, called the second signal. The cortical functional system, called the first signalling system, which reacts to the first signal, is common to animals and humans. The cortical system, called the second signalling system, which reacts to the second signal, is unique to humans. The activity of the second signaling system, neural activity closely related to human speech function, is developed during infant ontogeny and is established on the basis of the first signaling system or unconditional reflex.

Perceptual narrowing is a developmental process in which the brain uses environmental experience to shape perception. This process can improve the perception of things that people often experience by themselves, but can reduce their perception of things that they do not often touch. This phenomenon is the result of neural plasticity, including hebry learning and synaptic pruning. By these mechanisms, the more frequently used neural pathways are strengthened, making them more efficient; and those less frequently used paths become inefficient. This process is most pronounced during the sensitive stages of development. Human infants are inherently able to perceive a wide variety of stimuli, and as they age, they begin to selectively narrow these cognitions by categorizing the various stimuli in a more socio-cultural manner. Most of the research in this field has focused on face recognition and phoneme recognition of human infants.

Narrowing of perception is also associated with synaesthesia. At birth, infants have a wide range of abilities to discover similarities and differences between languages. Phonemes in different languages are different for infants less than 6 months, but as infants grow and their brains develop, they cannot distinguish between phonemes in non-native languages and phonemes in their native languages. This is presumably because infants often use their native language rather than gain more experience in the case of non-native languages. Studies have shown that this perceived narrowing occurs in the first year of life. Infants aged 6-8 months old have a greater ability to distinguish non-local sounds than infants aged 8-10 months old. At 12 months of age, infants begin to understand and manufacture languages in their native language, and after the first year of their birth, infants can only show a low level of perceptibility of phonological differences similar to adults.

Autistic children often exhibit social impairment, ultimately perception narrowing impairment, manifested in facial recognition and phoneme recognition; therefore, the pathogenic risk of the disease is detected in advance, and the detection of the facial recognition capability or the phoneme recognition capability is required before 12 months; since the infant cannot actively control the attention and the visual stimulation effect is not good, the face recognition ability detection cannot be performed, and only another detection, namely, phoneme recognition ability detection, can be performed.

The existing event-related potential stimulation method usually adopts a sound, light, electricity and other stimulation modes or a simple audio-visual stimulation mode designed according to a first signal system, does not contain voice materials and has no pertinence.

The autism is difficult to treat once the autism occurs, and the later discovery does not help the treatment, so the early screening of the autism is necessary; because the neural plasticity of the baby is the highest, early intervention is discovered and targeted voice training is carried out, so that the morbidity risk can be reduced.

Therefore, how to provide a targeted physical detection method containing voice materials becomes a problem to be solved in the industry.

Disclosure of Invention

In view of the shortcomings of the prior art, the present invention provides a physical detection method for early screening of brain function narrowing, which contains voice material and is targeted.

In order to achieve the above object, the present invention provides a physical examination method for early screening of narrowing of brain function, the physical examination method comprising:

step S1: stimulating and arranging; adopting a voice material as stimulation, wherein the length of the voice material is 230-300 ms, and the stimulation interval is not less than 700 ms;

step S2: collecting data; randomly playing a plurality of stimuli through a loudspeaker, wherein the volume is 80dB, and the playing time is 15 minutes in total; recording continuous electroencephalogram signals by using a brain electrical amplifier (Rainjet) by taking the left and right earlobes as reference electrodes; amplifying the EEG signals by an amplifier, extracting the EEG signals in a segmented manner, and recording the EEG signals after correction;

step S3: processing data; superposing and averaging the recorded electroencephalogram signals, and respectively calculating the average values of the left and right brain areas to obtain two curves;

step S4: waveform identification; searching and identifying the trough of the curve, and automatically extracting the latency and amplitude of the curve.

In the present invention, the subject is an infant before 12 months.

In the present invention, in step S2, when the stimulation is played, the experimenter blows a balloon in front of the infant to attract his or her attention and to increase the tolerance of the infant to the electrode sensor.

In the present invention, in step S3, in view of no main effect or mutual influence on the respective conditions, two curves are displayed by the shuffle conditions for each hemisphere; there are 4 leads per brain region.

The invention belongs to the field of neuroelectrophysiology, and relates to an event-related potential (ERP) technology, which can be used for professional recording or synthesizing voice materials; in the process of playing stimulation, late negative slow waves related to language disorder are collected through electrodes on the scalp of the brain, and the method can be used for early screening of the risk of language disorder and autism.

The difficulty of the invention lies in finding out an effective paradigm and detection conditions suitable for Chinese infants, designing a detection scheme according to age and cognitive level, selecting applicable voice materials, and controlling the time precision of voice stimulation in a millisecond range; once the materials are selected improperly and the time control is not good, the negative slow wave at the late stage of the event-related potential is difficult to induce, and the expected screening effect cannot be achieved.

The invention screens and pairs voice materials which can be understood by infants based on a second signal system specific to human beings and a development rule of narrowed phoneme perception of the infants to serve as event-related potential stimulation materials, improves an international universal strange ball paradigm (Oddball), and forms a passive double strange ball paradigm by special arrangement.

Aiming at the current situation that once the autism is developed, only early intervention and targeted voice training can be performed, and no autism early screening tool is available at home and abroad, the invention adopts a method of stimulating the relevant potential of the induced event by voice to discover the risk of developing the autism in advance, fills the blank of physical detection and early screening of the autism at home and abroad, and thus achieves the purpose of early discovery and early treatment.

According to another embodiment of the present invention, in step S1, the stimulation interval is a time interval from the end of the previous stimulation to the start of the next stimulation.

According to another embodiment of the present invention, in step S1, the stimulus includes a standard stimulus and a deviation stimulus.

In the scheme, the voice material adopted by the standard stimulation is Sichuan dialect/dia/(diadia overlapped sound, which is the meaning of grandpa), and accounts for 80% of the total quantity of all stimulation.

The bias stimulus uses speech material in local and non-local languages:

the local voice is mandarin/ta/(such as tower pronunciation), and the stimulation adopted as the voice material accounts for 10% of the total quantity of all stimulation;

the non-native speech is Shaanxi dialect/da/(dada syllabic, such as the meaning of largely grand-Bo), and the stimulus that is used as speech material accounts for 10% of the total number of all stimuli.

According to another embodiment of the present invention, at least 2 standard stimuli are played before the offset stimulus is played.

According to another embodiment of the present invention, step S2 is performed in a sound attenuation chamber of an electronic shield.

According to another embodiment of the invention, in step S2, when the electroencephalogram signal is amplified by the amplifier, the band pass is 0.1-100 Hz, and the digitization rate is 250 Hz.

According to another embodiment of the present invention, in step S2, the segmented extracted portions of the electroencephalogram signal include 100ms before stimulation and 700ms after stimulation.

According to another embodiment of the invention, in step S2, the extracted electroencephalogram signal is low-pass filtered at 30Hz, and baseline correction is performed with the average value of 100ms before stimulation as the baseline; after correction, the left and right regions are recorded by the electrodes in the anterior brain region, and the left and right regions are the left frontal-central region and the right frontal-central region, respectively.

According to another embodiment of the present invention, in step S3, only the standard stimulus located before the deviation stimulus participates in the superposition; the 3 stimuli were put together for mean overlay.

According to another embodiment of the present invention, in step S4, the negative slow wave is identified within 550ms and 250-550 ms with reference to the two-sided papilla.

In the scheme, when average reference is carried out, the average waveform takes the average value of all leads as reference again, and is identified within 300-700 ms.

Compared with the prior art, the invention has the following beneficial effects:

the invention can be used for early screening of the risk of language disorder and autism; the method of inducing the event-related potential by voice stimulation is adopted, late negative slow waves related to language disorder are collected through electrodes on the scalp of the brain, and the morbidity risk of the autism is found in advance; fills the blank of the early screening of the physical detection of autism at home and abroad, thereby achieving the aim of early detection and early treatment.

The present invention will be described in further detail with reference to the accompanying drawings.

Drawings

FIG. 1 is a schematic view of an electrode mounting position in the physical inspection method of example 1;

fig. 2 is a schematic diagram of a stimulation pattern of the physical detection method of example 1.

Detailed Description

Example 1

The embodiment provides a physical detection method for early screening of brain function narrowing, which comprises the following steps:

preparation work: registering the name and the month age of the detected baby on software before detection, and selecting a stimulation scheme; the grouping conditions are as follows: the age of the subject infant is within 12 months; exclusion conditions: a hearing impaired infant.

Step S1: stimulating and arranging; the voice material is used as the stimulus, the length of the voice material is 230-300 ms, and the stimulus interval is 700 ms.

The total number of voice stimulation is 600; the stimulus interval is the time interval from the end of the last stimulus to the start of the next stimulus.

The stimuli include standard stimuli and deviation stimuli.

The speech material used for the standard stimulation is Sichuan dialect/dia/(diadia overlapped sound, which is the meaning of grandpa), accounting for 80% of the total number of all stimulations.

The speech material adopted by the bias stimulus is a local language and a non-local language:

the non-native speech is Shanxi dialect (unviced)/da/(dada folding, such as the meaning of largely grand Boby), and the stimulus that is adopted as the speech material accounts for 10% of the total number of all stimuli.

Step S2: collecting data; randomly playing a plurality of stimuli through a loudspeaker, wherein the volume is 80dB, and the playing time is 15 minutes in total; recording continuous electroencephalogram signals by using a brain electrical amplifier (Rainjet) by taking the left and right earlobes as reference electrodes; the EEG signals are amplified by an amplifier, extracted in segments, corrected and recorded.

Step S2 is performed in the noise elimination chamber of the electronic shield; the 3 standard stimuli were played before the offset stimuli.

When stimulation is played, experimenters blow balloons in front of infants to attract the attention of the infants, and the tolerance of the infants to the electrode sensor is increased; wearing an electrode cap on the infant to be detected, and installing a sponge electrode soaked with saline water; as shown in figure 1, at the electrode position in the anterior brain region, the electrodes are installed according to 10/10 system in two areas: left frontal-central region (FC1, FC5, C3, C5) and right frontal-central region ((FC2, FC6, C4, C6).

After the installation, wearing an earphone on the infant to be detected, wherein the impedance of the electrode is lower than 5 kilo-ohms; as shown in fig. 2, the EEG is started to see whether the waveform is stable, after the waveform is stable, the ERP is started to collect and simultaneously play the stimulation, the baby to be tested is required to sit on the leg of the mother or the high chair, and after all the stimulation is played, the original data, namely the continuous electroencephalogram signal, is saved.

When the EEG signal is amplified by the amplifier, the band-pass is 50Hz, and the digitization rate is 250 Hz.

The part of the electroencephalogram signal which is extracted in a segmented mode comprises 100ms before stimulation and 700ms after stimulation.

The extracted electroencephalogram signals are subjected to low-pass filtering at 30Hz, and the average value of 100ms before stimulation is taken as a baseline to carry out baseline correction; after correction, the left and right regions are recorded by the electrodes in the anterior brain region, and the left and right regions are the left frontal-central region and the right frontal-central region, respectively.

Step S3: processing data; and (4) superposing and averaging the recorded electroencephalogram signals, and respectively calculating the average values of the left and right brain areas to obtain two curves.

Two curves are displayed for each hemisphere by perturbing the conditions, given that there is no main effect and interaction on the respective conditions; there are 4 leads per brain region.

Only the standard stimuli located before the deviation stimuli participate in the superposition; the 3 stimuli were put together for mean overlay.

After the band-pass filtering of 1-30 Hz, the wave trough is searched in the range of 250-550 milliseconds in the filtered waveform by taking the papilla on both sides as reference to identify the wave trough as a negative slow wave, or the negative slow wave is automatically marked, and the latent period and the wave amplitude are automatically extracted.

Example 2

This embodiment is different from embodiment 1 in that: in step S4, average reference is performed without taking bilateral mastoids as reference; the average waveform is re-referenced to the average of all leads and identified within 300-700 ms.

Example 3

This embodiment is different from embodiment 1 in that: the standard stimulus adopts a Fan language (voice)

Accounting for 80% of the total number of all stimuli.

The local voice is English combination (non-voice)/ta/, and the stimulation adopting the local voice as the voice material accounts for 10% of the total quantity of all stimulation;

the non-native speech was Sanskrit combination (not voice)/da/, with the stimuli used as speech material accounting for 10% of the total number of all stimuli.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to limit the scope of the invention. It will be appreciated by those skilled in the art that various modifications may be made without departing from the scope of the invention, and it is intended to cover all such modifications as fall within the true scope of the invention.

Claims

1. A non-diagnostic physical test method for early screening of narrowing of brain function, wherein the physical test method comprises:

step S4: waveform identification; searching and identifying the trough of the curve, and automatically extracting the latency and amplitude of the trough;

in the step S1, the stimulus includes a standard stimulus and a deviation stimulus; the voice materials adopted by the deviation stimulation are a local language and a non-local language;

in step S2, the part of the electroencephalogram signal which is extracted in segments includes 100ms before stimulation and 700ms after stimulation; playing at least 2 standard stimuli before playing the deviation stimulus;

in step S3, only the standard stimulus before the deviation stimulus participates in the superposition;

the early screening described above is applicable to infants aged 6-12 months.

2. The non-diagnostic physical assay of claim 1, wherein in step S1, the stimulus interval is the time interval from the end of the last stimulus to the start of the next stimulus.

3. The non-diagnostic physical test method of claim 1, wherein the differential stimulus is played before at least 2 standard stimuli are played.

4. The non-diagnostic physical test method of claim 1, wherein said step S2 is performed in an electronically shielded anechoic chamber.

5. The non-diagnostic physical examination method of claim 1, wherein in step S2, when the electroencephalogram signal is amplified by an amplifier, the band pass is 0.1-100 Hz, and the digitization rate is 250 Hz.

6. The non-diagnostic physical examination method of claim 1, wherein in step S2, the extracted electroencephalogram signal is low-pass filtered at 30Hz, and baseline correction is performed with the average value of 100ms before stimulation as a baseline; after correction, the left and right regions are recorded by the electrodes in the anterior brain region, and the left and right regions are the left frontal-central region and the right frontal-central region, respectively.

7. The non-diagnostic physical assay method as set forth in claim 1, wherein in step S4, the negative slow wave is recognized within 550ms and 250-550 ms with reference to the bilateral papilla.