CN108300747A - A method of it is pre-processed using sodium chlorite and improves reed enzymatic saccharification efficiency - Google Patents
A method of it is pre-processed using sodium chlorite and improves reed enzymatic saccharification efficiency Download PDFInfo
- Publication number
- CN108300747A CN108300747A CN201810118862.2A CN201810118862A CN108300747A CN 108300747 A CN108300747 A CN 108300747A CN 201810118862 A CN201810118862 A CN 201810118862A CN 108300747 A CN108300747 A CN 108300747A
- Authority
- CN
- China
- Prior art keywords
- sodium chlorite
- reed
- enzymatic saccharification
- saccharification efficiency
- feature exists
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 38
- 235000014676 Phragmites communis Nutrition 0.000 title claims abstract description 35
- UKLNMMHNWFDKNT-UHFFFAOYSA-M sodium chlorite Chemical compound [Na+].[O-]Cl=O UKLNMMHNWFDKNT-UHFFFAOYSA-M 0.000 title claims abstract description 28
- 229960002218 sodium chlorite Drugs 0.000 title claims abstract description 28
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 18
- 108090000790 Enzymes Proteins 0.000 claims abstract description 35
- 102000004190 Enzymes Human genes 0.000 claims abstract description 35
- 230000007062 hydrolysis Effects 0.000 claims abstract description 31
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 31
- 239000002994 raw material Substances 0.000 claims abstract description 25
- 238000006243 chemical reaction Methods 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 33
- 239000000243 solution Substances 0.000 claims description 14
- 108010059892 Cellulase Proteins 0.000 claims description 9
- 229940106157 cellulase Drugs 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 3
- 239000001509 sodium citrate Substances 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 108010084185 Cellulases Proteins 0.000 claims description 2
- 102000005575 Cellulases Human genes 0.000 claims description 2
- 230000009849 deactivation Effects 0.000 claims description 2
- 238000007781 pre-processing Methods 0.000 claims 1
- 230000035484 reaction time Effects 0.000 claims 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 15
- 239000002028 Biomass Substances 0.000 abstract description 5
- 229920005610 lignin Polymers 0.000 abstract description 4
- 238000005406 washing Methods 0.000 abstract description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 28
- 239000001913 cellulose Substances 0.000 description 22
- 229920002678 cellulose Polymers 0.000 description 22
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 15
- 239000008103 glucose Substances 0.000 description 15
- 229920002488 Hemicellulose Polymers 0.000 description 14
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 14
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 14
- 239000002023 wood Substances 0.000 description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 5
- 239000002253 acid Substances 0.000 description 3
- 238000005422 blasting Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- 150000002972 pentoses Chemical class 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004146 energy storage Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003345 natural gas Substances 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 229920001221 xylan Polymers 0.000 description 1
- 150000004823 xylans Chemical class 0.000 description 1
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/02—Glucose; Glucose-containing syrups obtained by saccharification of cellulosic materials
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K13/00—Sugars not otherwise provided for in this class
- C13K13/002—Xylose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Emergency Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to the preconditioning technique fields of biomass, and in particular to a method of it is pre-processed using sodium chlorite and improves reed enzymatic saccharification efficiency.Reed after crushing is mixed with sodium chlorite solution first, is sealed after being placed in reaction bulb, heating reaction postcooling；Then step 1 reaction solution after cooling is separated by solid-liquid separation, filter residue is washed with water to neutrality and is used for enzyme hydrolysis.This method is pre-processed using reed as raw material by sodium chlorite solution, and pretreated raw material is obtained, and then carries out Enzymatic hydrolysis to pretreated raw material.Reaction condition is mild, and enzyme hydrolysis is directly carried out by simply washing after reaction, and the removal rate of lignin is high.
Description
Technical field
The present invention relates to the preconditioning technique fields of biomass, and in particular to a kind of to improve reed using sodium chlorite pretreatment
The method of enzymatic saccharification efficiency.
Background technology
With the fast development of global economy, the consumption of the energy increases considerably, and fossil energy storage capacity is limited, no
It is renewable.Energy crisis is increasingly sharpened, and is found and new is had become inexorable trend instead of the energy.The biomass that the whole world generates every year
5% contained by energy be equivalent to demand of the mankind to oil and natural gas, therefore Bio-energy Development becomes the present age
Important front edge project in field of scientific study.
Reed is perennial tall and big herbaceous biomass raw material, and growth amount is more than most of xylophyta, has renewable energy
All good characteristics of source plant, such as：Fertility is strong, and unit interval unit area yield of biomass is high, and drought resistance is good, resistance to
Saline and alkaline, of less demanding to soil, distribution is wide, and the more other plants of content of cellulose are high, have very big application potential.Reed and other wood
Matter fibrous raw material is the same, is mainly made of cellulose, hemicellulose and three big element of lignin, wherein cellulose and hemicellulose
Monosaccharide can be generated by enzyme hydrolysis, then be further converted into bio-fuel or the chemicals of high added value.Lignin cannot
It is hydrolyzed, and form protective layer around cellulose and limit cellulose hydrolysis, the crystalline texture of cellulose itself is added, is made
Natural lignocellulose raw material directly to carry out efficiency when enzyme hydrolysis very low (10%~20%).
Pretreatment can change the structure of natural fiber, reduce the crystallinity of cellulose, destroy or slough hemicellulose and wood
Quality increases its porosity, that is, increases the contact area of enzyme and cellulose, to promote enzyme hydrolysis.
The preprocess method of reed mainly has steam blasting pretreatment, dilute acid pretreatment, alkaline process pretreatment etc. at present.Tianjin section
Skill college journal, in 2016,31,45-50 the pretreatment of report steam blasting can significantly improve the cellulose hydrolyzation efficiency of reed,
The enzyme hydrolysis efficiency of untreated reed raw material is only 14.4%, and after steam blasting pre-processes, the cellulose hydrolyzation of reed material is imitated
Rate can reach 88.9%, but the method needs carry out under high temperature and high pressure environment, and energy consumption is higher, to equipment requirement
Height is unfavorable for producing in enormous quantities.Agricultural University of the Inner Mongol, 2011, Liao irrigates in day sweat Master's thesis to be located in advance using 1.5% sulfuric acid
After managing reed, 13% or so cellulose and 80% or more hemicellulose is dissolved in pretreatment fluid, mainly with glucose and xylose
Form exist, need neutralize after be fermented utilization, the salt ethyl alcohol multipair greatly or butanol fermentation process for neutralizing formation have inhibition
Effect, and it is complicated for operation.Hua Zhong Agriculture University, 2011, in Xu Nin's master thesis, utilize NaOH pretreatment reed former
Material, it has been investigated that with the raising of naoh concentration, pentose increases considerably in supernatant, the increasing degree of hexose
Smaller, the pentose in enzymolysis liquid reaches maximum when naoh concentration is 1.0%, but the method is the same as sulfur acid pretreatment class
Seemingly, the sugar in supernatant recycles complicated.
In conclusion seeking that a kind of reaction condition is mild, the pretreatment of reed raw material availability and enzymatic saccharification efficiency is improved
Method is very necessary.
Invention content
The object of the present invention is to provide a kind of reaction condition is mild, sodium chlorite pretreatment is utilized to improve reed enzymatic saccharification effect
The method of rate, this method are pre-processed using reed as raw material by sodium chlorite solution, pretreated raw material are obtained, then to pre-
Treated, and raw material carries out Enzymatic hydrolysis.
Technical solution of the invention is to provide a kind of pre-processed using sodium chlorite and improves reed enzymatic saccharification efficiency
Method includes the following steps：
Step 1：Reed after crushing is mixed with sodium chlorite solution, is sealed after being placed in reaction bulb, it is cold after heating reaction
But；
Step 2：Step 1 reaction solution after cooling is separated by solid-liquid separation, filter residue is washed with water to neutrality and is used for enzyme hydrolysis.
Preferably, the mass fraction of sodium chlorite solution is 2~6% in step 1.
Preferably, the solid-liquid ratio of reed and sodium chlorite solution are 1:6~1:10.
Preferably, water bath heating temperature be 80 DEG C, water-bath time be 0.5~for 24 hours.
Preferably, raw material reed in step 1 is crushed to 60 mesh.
Preferably, it is heated using water-bath.
Preferably, heating temperature is 80 DEG C, and water-bath time is for 24 hours.
Preferably, enzyme hydrolysis process is specially：The pretreated raw material of a certain amount of step 2 is weighed in conical flask, is added
Enter sodium citrate buffer, cellulase or cellulase and zytase that pH is equal to 5.0, is placed in conical flask after shaking up
Hydrolyze 48h in constant-temperature table at 50 DEG C, enzyme deactivation in 10 minutes in boiling water bath obtains hydrolyzate.
Preferably, the material quality of enzyme hydrolysis a concentration of 2%, every gram of dry matter cellulase dosage are 10~60FPU.
Preferably, the material quality of enzyme hydrolysis a concentration of 2%, every gram of dry matter enzyme dosage are 10FPU cellulases and 2mg
Zytase.
The beneficial effects of the invention are as follows：
1, the treatment fluid that the present invention uses is sodium chlorite solution, and reaction condition is mild, straight by simply washing after reaction
Row enzyme hydrolysis is tapped into, the removal rate of lignin is high；
2, the present invention can retain a large amount of cellulose and hemicellulose, and enzymatic saccharification effect is good, and raw material availability is high；
3, present invention process is easy to operate, low energy consumption, has industrial mass production foreground.
Specific implementation mode
Further specific detailed description is done to the present invention below in conjunction with specific embodiment, but embodiments of the present invention are unlimited
In this.
Embodiment one
It weighs reed that over dry weight is 60 grams to be placed in a reaction flask, according to solid-liquid ratio 1:10 are added the sub- chlorine of mass fraction 2%
Acid sodium solution seals reaction bulb, reaction bulb is put into 80 DEG C of water-bath and is pre-processed 0.5 hour.After pretreatment immediately
Let off steam, discharging and cooling, centrifuge are separated by solid-liquid separation, and liquid is discarded, with distilled water, by pretreated feedstock, (residue is solid
Body) washing is to being used for biological enzyme hydrolysis after neutrality.
1 gram of pretreated feedstock is weighed in conical flask, the cellulase of 10FPU/g DM (dry matter) is added, is eventually adding
Sodium citrate buffers of the pH equal to 5.0 makes substrate mass concentration be 2%, shakes up to be placed in constant-temperature table and is hydrolyzed at 50 DEG C
48h.Being boiled 10 minutes in boiling water bath after the completion of hydrolysis makes enzyme inactivate.It is cooled to room temperature, hydrolyzate is centrifuged, supernatant uses
High performance liquid chromatography detection monosaccharide calculates the hydrolysis yield of pretreated feedstock each component removal rate and sugar according to following formula：
Cellulose and xylan content in formula in substrate are measured according to National Renewable Energy laboratory method；
It is computed, pretreated raw material wood element removal rate is 46.6%, cellulose and hemicellulose loss late difference
For 3.5% and 4.2%；Glucose yield is 32.6% after enzyme hydrolysis, and xylose yield is 20.9%.
Embodiment two
Pretreatment and enzymatic hydrolysis reaction condition and embodiment one are essentially identical, and it is a concentration of that difference lies in sodium chlorite solutions
4%.It is computed, pretreated raw material wood element removal rate is 59.6%, and cellulose and hemicellulose loss late are respectively
5.0% and 6.2%；Glucose yield is 40.1% after enzyme hydrolysis, and xylose yield is 21.5%.
Embodiment three
Pretreatment and enzymatic hydrolysis reaction condition and embodiment one are essentially identical, and it is a concentration of that difference lies in sodium chlorite solutions
6%.It is computed, pretreated raw material wood element removal rate is 61.6%, and cellulose and hemicellulose loss late are respectively
5.4% and 7.2%；Glucose yield is 43.2% after enzyme hydrolysis, and xylose yield is 23.9%.
Example IV
Pretreatment and enzymatic hydrolysis reaction condition and embodiment three are essentially identical, are 1h difference lies in pretreatment time.Through meter
It calculates, pretreated raw material wood element removal rate is 67.0%, and cellulose and hemicellulose loss late are respectively 7.5% He
9.1%；Glucose yield is 50.7% after enzyme hydrolysis, and xylose yield is 29.3%.
Embodiment five
Pretreatment and enzymatic hydrolysis reaction condition and embodiment three are essentially identical, are 6h difference lies in pretreatment time.Through meter
It calculates, pretreated raw material wood element removal rate is 71.4%, and cellulose and hemicellulose loss late are respectively 13.1% He
13.7%；Glucose yield is 72.7% after enzyme hydrolysis, and xylose yield is 43.7%.
Embodiment six
Pretreatment and enzymatic hydrolysis reaction condition and embodiment three are essentially identical, are 12h difference lies in pretreatment time.Through meter
It calculates, pretreated raw material wood element removal rate is 68.8%, and cellulose and hemicellulose loss late are respectively 13.5% He
17.3%；Final glucose yield is 82.2% after enzyme hydrolysis, and xylose yield is 54.9%.
Embodiment seven
Pretreatment and enzymatic hydrolysis reaction condition and embodiment three are essentially identical, are for 24 hours difference lies in pretreatment time.Through meter
It calculates, pretreated raw material wood element removal rate is 66.6%, and cellulose and hemicellulose loss late are respectively 13.7% He
17.6%；Final glucose yield is 85.0% after enzyme hydrolysis, and xylose yield is 63.4%.
Embodiment eight
Pretreatment and enzymatic hydrolysis reaction condition and embodiment six are essentially identical, are 20FPU/ difference lies in cellulase dosage
g DM.It is computed, final glucose yield is 88.8% after enzyme hydrolysis, and xylose yield is 65.7%.
Embodiment nine
Pretreatment and enzymatic hydrolysis reaction condition and embodiment six are essentially identical, are 40FPU/ difference lies in cellulase dosage
g DM.It is computed, final glucose yield is 100.0% after enzyme hydrolysis, and xylose yield is 74.3%.
Embodiment ten
Pretreatment and enzymatic hydrolysis reaction condition and embodiment six are essentially identical, are 60FPU/ difference lies in cellulase dosage
g DM.It is computed, final glucose yield is 102.5% after enzyme hydrolysis, and xylose yield is 76.3%.
Embodiment 11
Pretreatment and enzymatic hydrolysis reaction condition and embodiment six are essentially identical, are 1 difference lies in solid-liquid ratio：8.It is computed,
Pretreated raw material wood element removal rate is 70.2%, and cellulose and hemicellulose loss late are respectively 12.5% He
13.1%；Final glucose yield is 70.4% after enzyme hydrolysis, and xylose yield is 40.5%.
Embodiment 12
Pretreatment and enzymatic hydrolysis reaction condition and embodiment six are essentially identical, are 1 difference lies in solid-liquid ratio：6.It is computed,
Pretreated raw material wood element removal rate is 68.7%, and cellulose and hemicellulose loss late are respectively 12.3% He
12.8%；Final glucose yield is 70.1% after enzyme hydrolysis, and xylose yield is 39.8%.
Embodiment 13
Pretreatment and enzymatic hydrolysis reaction condition and embodiment six are essentially identical, and difference lies in be additionally added to 2mg/g DM's
Zytase, final glucose yield are 93.6, and xylose yield is 65.2%.
Embodiment 14
Pretreatment and enzymatic hydrolysis reaction condition and embodiment seven are essentially identical, and difference lies in be additionally added to 2mg/gDM's
Zytase, final glucose yield are 93.7%, and xylose yield is 69.9%.
Claims (10)
1. a kind of pre-processing the method for improving reed enzymatic saccharification efficiency using sodium chlorite, include the following steps：
Step 1：Reed after crushing is mixed with sodium chlorite solution, is sealed after being placed in reaction bulb, heating reaction postcooling；
Step 2：Step 1 reaction solution after cooling is separated by solid-liquid separation, filter residue is washed with water to neutrality and is used for enzyme hydrolysis.
2. according to claim 1 pre-process the method for improving reed enzymatic saccharification efficiency using sodium chlorite, feature exists
In：The mass fraction of sodium chlorite solution is 2~6% in step 1.
3. according to claim 2 pre-process the method for improving reed enzymatic saccharification efficiency using sodium chlorite, feature exists
In：The solid-liquid ratio of reed and sodium chlorite solution are 1 in step 1:6~1:10.
4. according to claim 3 pre-process the method for improving reed enzymatic saccharification efficiency using sodium chlorite, feature exists
In：In step 1 heating temperature be 80 DEG C, the reaction time be 0.5~for 24 hours.
5. according to claim 4 pre-process the method for improving reed enzymatic saccharification efficiency using sodium chlorite, feature exists
In：Raw material reed in step 1 is crushed to 60 mesh.
6. according to claim 5 pre-process the method for improving reed enzymatic saccharification efficiency using sodium chlorite, feature exists
In：It is heated using water-bath.
7. according to claim 6 pre-process the method for improving reed enzymatic saccharification efficiency using sodium chlorite, feature exists
In：Heating temperature is 80 DEG C, and water-bath time is for 24 hours.
8. according to claim 7 pre-process the method for improving reed enzymatic saccharification efficiency using sodium chlorite, feature exists
In：Enzyme hydrolysis process is specially：The pretreated raw material of a certain amount of step 2 is weighed in reaction bulb, pH is added and is equal to 5.0
Sodium citrate buffer, cellulase or cellulase and zytase, shake up to be placed in constant-temperature table and are hydrolyzed at 50 DEG C
48h, enzyme deactivation in 10 minutes in boiling water bath, obtains hydrolyzate.
9. according to claim 8 pre-process the method for improving reed enzymatic saccharification efficiency using sodium chlorite, feature exists
In：The material quality a concentration of 2% of enzyme hydrolysis, every gram of dry matter (DM) enzyme dosage are 10~60FPU.
10. according to claim 9 pre-process the method for improving reed enzymatic saccharification efficiency using sodium chlorite, feature exists
In：The material quality a concentration of 2% of enzyme hydrolysis, every gram of dry matter enzyme dosage are 10FPU cellulases and 2mg zytases.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810118862.2A CN108300747B (en) | 2018-02-06 | 2018-02-06 | Method for improving saccharified efficiency of silvergrass enzymolysis by utilizing sodium chlorite pretreatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810118862.2A CN108300747B (en) | 2018-02-06 | 2018-02-06 | Method for improving saccharified efficiency of silvergrass enzymolysis by utilizing sodium chlorite pretreatment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108300747A true CN108300747A (en) | 2018-07-20 |
| CN108300747B CN108300747B (en) | 2020-11-27 |
Family
ID=62864417
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810118862.2A Expired - Fee Related CN108300747B (en) | 2018-02-06 | 2018-02-06 | Method for improving saccharified efficiency of silvergrass enzymolysis by utilizing sodium chlorite pretreatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108300747B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283863A (en) * | 2019-07-08 | 2019-09-27 | 南京林业大学 | A method of preparing fermentable sugar from softwood |
| CN111763695A (en) * | 2020-07-28 | 2020-10-13 | 暨南大学 | Method for preparing ethanol fuel by pretreating bagasse with sodium phosphate |
| CN116555359A (en) * | 2023-06-25 | 2023-08-08 | 齐鲁工业大学(山东省科学院) | Method for producing fuel ethanol by bioconversion of poplar biomass |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063100A (en) * | 2015-09-01 | 2015-11-18 | 常州大学 | Method for pretreating non-lumber fibrous materials by utilizing basic salt in combination with hydrogen peroxide |
| CN107164424A (en) * | 2017-07-12 | 2017-09-15 | 天津科技大学 | It is a kind of to aoxidize the lignocellulose pretreatment method that delignification improves enzyme hydrolysis rate |
| CN107502636A (en) * | 2017-10-13 | 2017-12-22 | 西北农林科技大学 | A kind of method using ammoniacal liquor Cold pretreatment hybrid Chinese pennisetum |
-
2018
- 2018-02-06 CN CN201810118862.2A patent/CN108300747B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105063100A (en) * | 2015-09-01 | 2015-11-18 | 常州大学 | Method for pretreating non-lumber fibrous materials by utilizing basic salt in combination with hydrogen peroxide |
| CN107164424A (en) * | 2017-07-12 | 2017-09-15 | 天津科技大学 | It is a kind of to aoxidize the lignocellulose pretreatment method that delignification improves enzyme hydrolysis rate |
| CN107502636A (en) * | 2017-10-13 | 2017-12-22 | 西北农林科技大学 | A kind of method using ammoniacal liquor Cold pretreatment hybrid Chinese pennisetum |
Non-Patent Citations (3)
| Title |
|---|
| RISHI GUPTA等: "Evaluation of pretreatment methods in improving the enzymatic saccharification of cellulosic materials", 《CARBOHYDRATE POLYMERS》 * |
| 张美云等: "《非木材纤维乙醇法制浆原理与技术》", 30 August 2012 * |
| 邓长江等: "荻半纤维素化学结构的研究", 《天津造纸》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283863A (en) * | 2019-07-08 | 2019-09-27 | 南京林业大学 | A method of preparing fermentable sugar from softwood |
| CN111763695A (en) * | 2020-07-28 | 2020-10-13 | 暨南大学 | Method for preparing ethanol fuel by pretreating bagasse with sodium phosphate |
| CN116555359A (en) * | 2023-06-25 | 2023-08-08 | 齐鲁工业大学(山东省科学院) | Method for producing fuel ethanol by bioconversion of poplar biomass |
| CN116555359B (en) * | 2023-06-25 | 2023-12-08 | 齐鲁工业大学(山东省科学院) | Method for producing fuel ethanol by bioconversion of poplar biomass |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108300747B (en) | 2020-11-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104498562A (en) | Method for pretreating agricultural waste biomass by adopting sodium hydroxide/urea/water system | |
| CN106496351A (en) | A kind of method of nutrient substance in high efficiency extraction Sargassum | |
| CN108300747A (en) | A method of it is pre-processed using sodium chlorite and improves reed enzymatic saccharification efficiency | |
| CN101979641A (en) | Method for refining sugar by rapidly degrading reed fibers | |
| CN105695524A (en) | Method for producing biodiesel by means of lignocellulose material | |
| Chen et al. | Prospects for bioethanol production from macroalgae | |
| CN105385722B (en) | A method of pretreatment wood fiber biomass improves its sugar yield | |
| CN104726502A (en) | Method for biologically preparing ethanol and coproducing chitosan from cellulose waste | |
| CN104561129A (en) | Enzymolysis saccharification processing method of straw | |
| CN103352057B (en) | Method for preparing fuel ethanol by taking bamboo shoot processing residues as raw materials | |
| CN103045680A (en) | Comprehensive utilization method of lignocellulose biomass | |
| CN103045697A (en) | Comprehensive utilization method of lignocellulose biomass | |
| CN103045677A (en) | Comprehensive utilization method of lignocellulose biomass | |
| CN107502636B (en) | Method for pretreating hybrid pennisetum alopecuroides at low temperature by using ammonia water | |
| CN104531806A (en) | Method for producing fermentable sugar | |
| CN103045696B (en) | Comprehensive utilization method of lignocellulose biomass | |
| CN101289677A (en) | Process for preparing ethanol by using cellulose-containing raw material | |
| CN103409483B (en) | Method for preparing fermentable sugar from water-washed alkaline pretreated plant fiber raw material | |
| CN113718002B (en) | Method for preparing micromolecular galactomannan and galactomannan-oligosaccharide by using corncob alkali extraction residues | |
| CN103045694A (en) | Comprehensive utilization method of lignocellulose biomass | |
| CN103045687A (en) | Comprehensive utilization method of lignocellulose biomass | |
| CN103045688A (en) | Comprehensive utilization method of lignocellulose biomass | |
| CN103045698B (en) | Method for comprehensively utilizing lignocellulose biomass | |
| CN103045695B (en) | Comprehensive utilization method of lignocellulose biomass | |
| CN105950670A (en) | Method for preparing ethyl alcohol from schisandra chinensis stems |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201127 |