CN108294316A - A kind of enzymolysis of fish proteins polypeptide compound oral liquid and preparation method thereof - Google Patents
A kind of enzymolysis of fish proteins polypeptide compound oral liquid and preparation method thereof Download PDFInfo
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- CN108294316A CN108294316A CN201810081090.XA CN201810081090A CN108294316A CN 108294316 A CN108294316 A CN 108294316A CN 201810081090 A CN201810081090 A CN 201810081090A CN 108294316 A CN108294316 A CN 108294316A
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 133
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 121
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 121
- 239000007788 liquid Substances 0.000 title claims abstract description 120
- 108010028690 Fish Proteins Proteins 0.000 title claims abstract description 97
- 150000001875 compounds Chemical class 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 63
- 235000007516 Chrysanthemum Nutrition 0.000 claims abstract description 33
- 235000017784 Mespilus germanica Nutrition 0.000 claims abstract description 29
- 235000000560 Mimusops elengi Nutrition 0.000 claims abstract description 29
- 235000007837 Vangueria infausta Nutrition 0.000 claims abstract description 29
- 239000002781 deodorant agent Substances 0.000 claims abstract description 20
- 241000723353 Chrysanthemum Species 0.000 claims abstract description 14
- 229940100688 oral solution Drugs 0.000 claims abstract description 13
- 239000000284 extract Substances 0.000 claims abstract description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 45
- 239000002994 raw material Substances 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 244000182216 Mimusops elengi Species 0.000 claims description 26
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 21
- 244000189548 Chrysanthemum x morifolium Species 0.000 claims description 19
- 229940069445 licorice extract Drugs 0.000 claims description 19
- 235000021552 granulated sugar Nutrition 0.000 claims description 18
- 238000000855 fermentation Methods 0.000 claims description 17
- 230000004151 fermentation Effects 0.000 claims description 17
- 235000019658 bitter taste Nutrition 0.000 claims description 15
- 241000251468 Actinopterygii Species 0.000 claims description 12
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 12
- 239000004365 Protease Substances 0.000 claims description 10
- 239000000047 product Substances 0.000 claims description 10
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- 108091005804 Peptidases Proteins 0.000 claims description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 6
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- 235000020995 raw meat Nutrition 0.000 claims description 6
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 4
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- 244000269722 Thea sinensis Species 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
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- 244000303040 Glycyrrhiza glabra Species 0.000 description 1
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- 241000237852 Mollusca Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
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- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 230000003276 anti-hypertensive effect Effects 0.000 description 1
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- 229960003720 enoxolone Drugs 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 1
- 229960004949 glycyrrhizic acid Drugs 0.000 description 1
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 1
- 235000019410 glycyrrhizin Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000011477 liquorice Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- SBNFWQZLDJGRLK-UHFFFAOYSA-N phenothrin Chemical compound CC1(C)C(C=C(C)C)C1C(=O)OCC1=CC=CC(OC=2C=CC=CC=2)=C1 SBNFWQZLDJGRLK-UHFFFAOYSA-N 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- 210000000697 sensory organ Anatomy 0.000 description 1
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- 241000894007 species Species 0.000 description 1
- WNIFXKPDILJURQ-UHFFFAOYSA-N stearyl glycyrrhizinate Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C)CCC(C(=O)OCCCCCCCCCCCCCCCCCC)(C)CC5C4=CC(=O)C3C21C WNIFXKPDILJURQ-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention belongs to aquatic food field of deep.The purpose of the present invention is to provide a kind of a kind of suitable Fish protein enzymolysis polypeptide compound oral liquids of taste, the oral solution contains Fish protein enzymolysis polypeptide liquid, concentrated medlar juice, chrysanthemum inspissated juice, and the wherein weight of concentrated medlar juice and chrysanthemum inspissated juice accounts for 15 30%, the 3 8% of Fish protein enzymolysis polypeptide liquid weight respectively.Preparation method includes that prepared by fish protein polypeptide liquid, prepared by the de- bitter, concentrated medlar juice of polypeptide liquid deodorant, chrysanthemum inspissated juice extracts and compound adaptation step.The present invention by flavor mixing and nutrient equilibrium collocation prepare taste flavor is suitable, the higher fish protein polypeptide of nutritive value take orally liquid product, provide the new approaches that fish protein polypeptide is applied in food service industry.
Description
Technical field
The invention belongs to aquatic food field of deep, and in particular to a kind of enzymolysis of fish proteins polypeptide compound oral liquid and its
Preparation method.
Background technology
Polypeptide is connected a kind of compound for constituting by peptide chain by amino acid, i.e. ɑ-carboxyl in a molecule amino acid
A molecular water is removed with the ɑ in another molecule amino acid-amino and is condensed the amide to be formed, and the amido bond of formation is known as peptide bond.It is raw
Object active peptide refers to the small peptide for having the polypeptide of various biological function and being formed less than 10 amino acid, each active peptide is all
With unique amino acid sequence, it is different that different amino acid composition determines that active peptide has the function of.
There are abundant biological species, this just provides extensive source, ocean for marine organisms natural polypeptides in ocean
Mesichthyes polypeptide is the edible living resources of people's early start, contains abundant protein component, ocean Mesichthyes in vivo
Resourceful, type is various so that fish polypeptide has multiple biological activities, but has the biology of medical value from wherein exploitation
The research of active peptide is less.The natural activity peptide extracted from marine fishes has anti-hypertension, anti-oxidant, antitumor, drop blood
The effects that fat.
Proteinase hydrolization method is aminosal molecule to prepare a kind of common method of biologically active peptide, enzyme hydrolysis method because
To be mild with reaction condition, cost is relatively low, it is safe the advantages that and be concerned.It is needed during enzyme hydrolysis method to enzymolysis
The reaction conditions such as time, hydrolysis temperature, pH values, liquid-solid ratio and enzyme concentration optimize, and there is common protease in enzyme hydrolysis method
Papain, trypsase, bromelain, neutral proteinase, acid protease, basic protein and pepsin etc.,
Different protease are because restriction enzyme site and reaction condition are different, even if digesting same protein molecule, obtained polypeptide number
Mesh and sequence also differ greatly.The polypeptide that enzyme hydrolysis method obtains also has preferable water-soluble other than with multiple biological activities
Property, thermal stability, ph stability etc., can be widely used in food production, medicine and agriculture field.
Marine organisms polypeptide has caused extensively because it can potentially influence human health and reduce disease risks
Concern.This kind of polypeptide mostlys come from algae, fish, mollusk, Crustaceans and ocean byproduct etc..Marine organisms
Active peptide has extensive bioactivity because of its architectural characteristic, amino acid composition and sequence difference, including anti-oxidant work
Property, antihypertensive active, antibiotic property, immunological regulation, anti-inflammatory, antithrombotic and reduce cholesterol etc., these functions are macromoleculars
Physiological activity not available for albumen or free amino acid mixture.
Marine fish processing waste is mainly processed into fish meal, organic fertilizer and foliar fertilizer etc. at present, Utilizing Resource Pattern compared with
It is single.Therefore, intensive processing is carried out, develops into a kind of polypeptide oral liquor with nutritional supplementation effect, product can be improved
Added value increases its Utilizing Resource Pattern, to promote the development of marine fish secondary industry.
Invention content
The Fish protein enzymolysis activity that the purpose of the present invention is to provide a kind of tastes is suitable, nutritive value is high, suitable for people of all ages
Peptide oral liquid, and a kind of preparation method of the oral solution is provided in turn.
In order to achieve the above objectives, the technical solution adopted by the present invention is：A kind of Fish protein enzymolysis polypeptide compound oral liquid,
It is characterized in that：The oral solution contains Fish protein enzymolysis polypeptide liquid, concentrated medlar juice, chrysanthemum inspissated juice, wherein concentrated medlar juice and
The weight of chrysanthemum inspissated juice accounts for 15-30%, 3-8% of Fish protein enzymolysis polypeptide liquid weight respectively.
Further, in above-mentioned Fish protein enzymolysis polypeptide compound oral liquid also contain citric acid and white granulated sugar, citric acid and
The content of white granulated sugar is respectively Fish protein enzymolysis polypeptide liquid 0.1-0.3%, 3-5%.
Further, for the Fish protein enzymolysis polypeptide liquid in the oral solution using de- bitter fishy smell elimination process processing, described is de-
Bitter fishy smell elimination process adds yeast in referring to polypeptide liquid and ferments, and the fermentation includes that licorice extract is added into polypeptide liquid,
By polypeptide liquid and licorice extract mixed fermentation.
A kind of preparation method of Fish protein enzymolysis polypeptide compound oral liquid comprising the following steps：
1）Fish protein polypeptide liquid is obtained by digesting fish raw material preparation；
2）The de- hardship of fish protein polypeptide liquid deodorant
Fish protein enzymolysis polypeptide liquid is mixed with the licorice extract of its weight 1-3%, is fermented using yeast, inoculum of dry yeast is
The 0.1-0.5% of Fish protein enzymolysis polypeptide liquid weight, 16-19 DEG C of fermentation temperature, fermentation time 3-9d, carbon dioxide pressure are kept
0.12-0.3Mpa is made without Fish protein enzymolysis polypeptide liquid of the raw meat without bitter taste；
3）It is prepared by concentrated medlar juice：Matrimony vine finished product is taken, constant temperature impregnates 30-50min, filtering in 85 DEG C of pure water, and filtrate exists
The 50-60% of pure water weight is concentrated under reduced pressure at 50-60 DEG C；
4）Chrysanthemum inspissated juice extracts：Chrysanthemum tea finished product is taken, constant temperature impregnates 20-30min, filtering in 80 DEG C of pure water, and filtrate exists
The 50-60% of pure water weight is concentrated under reduced pressure at 50-60 DEG C；
5）Compound is allocated
By step 2）Prepare without Fish protein enzymolysis polypeptide liquid of the raw meat without bitter taste, step 3）The concentrated medlar juice and step 4 of preparation）
The chrysanthemum inspissated juice of preparation mixes, and wherein the weight of concentrated medlar juice and chrysanthemum inspissated juice accounts for Fish protein enzymolysis polypeptide liquid weight respectively
15-30%, 3-8% of amount；
6）By step 5）Mixed liquor filling and sterilization obtained, sealing are made.
Further, above-mentioned steps 5）In compound allotment further include that lemon juice and white granulated sugar are added into mixed liquor,
The content of middle citric acid and white granulated sugar is respectively 0.1-0.3%, 3-5% of Fish protein enzymolysis polypeptide liquid weight.
Further, described 1）In by digest fish raw material preparation obtain fish protein polypeptide liquid, further include enzymolysis before
The de- bitter step of the pre- deodorant of raw material, the step include being soaked in the sodium carbonate liquor for put into raw material after broken 3-5 times of raw material weight
Bubble 20-60 minutes, the raw material after immersion is rinsed well using clear water, then filters raw material by activated carbon.
Further, described 2）The de- hardship of fish protein polypeptide liquid deodorant, further includes the activation of yeast, which is：By Radix Glycyrrhizae
It puts into the water of 50-60 times of weight of its weight, water temperature controls 70-80 DEG C, decocts to carry 20-40 minutes and filter, filtered fluid is heated up
Be concentrated into Radix Glycyrrhizae weight 10-20 times obtains licorice extract, takes Fish protein, the licorice extract liter of enzymolysis polypeptide liquid weight 1-3%
Temperature boils postcooling to 30-35 DEG C, and yeast is added and is activated, and inoculum of dry yeast is the 0.1- of Fish protein enzymolysis polypeptide liquid weight
0.5%。
Further, the step 1）Fish protein polypeptide liquid is obtained by digesting fish raw material preparation, enzymolysis step is as follows：
1）Raw material is put into the pure water of 2.5-5 times of its weight, it is raw material to add animal protease and papain, additive amount
2 ‰ -4 ‰, digest 3.5-4.5h at 51-55 DEG C, filter out do not complete enzymolysis residue fish-skin slag；
2）It will be through step 1）Obtained enzymolysis liquid boils rapidly, and after keeping 1-2min, is cooled to room temperature with tap water water-bath；
3）By step 2）Solution at 2-4 DEG C, in separatory funnel stand 8-12h, after being layered, from separatory funnel lower end arrange
Go out to hang heavy layer, obtains clarified solution；
4）It is concentrated under reduced pressure：By step 3）30- is concentrated under reduced pressure at 55-60 DEG C, vacuum degree 0.09-0.10Mpa in obtained clarified solution
60min。
The enzymolysis of fish proteins that the present invention allocates Fish protein enzymolysis polypeptide liquid through sugar-acid ratio allotment and Chinese medicine compound
Polypeptide liquid nutritive value greatly improves, and is handled Fish protein enzymolysis polypeptide liquid by deodorant debitterized technique, can make product
Bitter off-flavors declined to a great extent, make mouthfeel smell and sense organ color and luster be more easy to be accepted.The present invention by flavor mixing and
Nutrient equilibrium collocation prepare taste flavor is suitable, the higher fish protein polypeptide of nutritive value take orally liquid product, provide fish egg
The new approaches that white polypeptide is applied in food service industry.
Specific implementation mode
Enzymolysis of fish proteins polypeptide compound oral liquid provided by the invention and preparation method thereof is done in detail with reference to embodiment
Thin description.
Embodiment 1
A kind of preparation method of enzymolysis of fish proteins polypeptide compound oral liquid comprising the following steps：
1, the pre- defishying of raw material
Fish processing fent, such as fish-bone, fish-skin are chosen, it is truncated into the fragment of 0.50cm by fish-bone along vertebrae, and fish-skin is broken
Until blanching 2min has white foam disappearance in boiling water together with fish-bone after broken, and fish-bone, fish-skin are filtered out rapidly, is cooled to room
Temperature.The above-mentioned raw material after blanching is put into sodium carbonate liquor and is impregnated 20-60 minutes, the specific time with raw material weight into
Row rationally determines that wherein sodium carbonate liquor weight is 3-5 times of raw material weight, sodium carbonate liquor concentration 1-3%.After immersion
Raw material is rinsed well using clear water, then by raw material by activated carbon channel by carry out peculiar smell adsorption, channel passes through time control
It is 20-30 minutes；
2, it digests
2.1 put into the raw material handled by step 1 in the pure water of 2.5-5 times of its weight, add animal protease and wood
Melon protease, additive amount are the 2 ‰ -4 ‰ of raw material, and 3.5-4.5h is digested at 51-55 DEG C, filter out the residue for not completing enzymolysis
Fish-skin slag；
2.2 boil rapidly the enzymolysis liquid obtained through step 2.1, and after keeping 1-2min, are cooled to room temperature with tap water water-bath；
2.3 by the solution of step 2.2 at 2-4 DEG C, in separatory funnel stand 8-12h, after being layered, from separatory funnel lower end arrange
Go out to hang heavy layer, obtains clarified solution；
2.4 being concentrated under reduced pressure：The clarified solution that step 2.3 obtains is concentrated under reduced pressure at 55-60 DEG C, vacuum degree 0.09-0.10Mpa
30-60min。
3, prepared by licorice extract
Extracting liquorice is put into after cleaning in the water of 50-60 times of weight of its weight, and water temperature controls 70-80 DEG C, is decocted and is put forward 20-40 minutes mistakes
Filtered fluid heating is concentrated into 10-20 times of Radix Glycyrrhizae weight and obtained by filter.
4, fermented yeast activates
Take step 2 gained clarified solution weight 1-3% licorice extract heat up boil postcooling to 30-35 DEG C then input beer
The compound barm of yeast, glucose yeast or both is activated, and wherein the weight of fermented yeast is that the outstanding heavy layer of step 2.3 is fixed
The 0.1-0.5% of object weight；
5, it ferments
Licorice extract after step 4 fermented yeast is activated is mixed with the clarified solution that step 2 obtains, closed container, control hair
16-19 DEG C of ferment temperature, fermentation time 3-9d, carbon dioxide pressure keep 0.12-0.3Mpa up to Fish protein of no raw meat without bitter taste
Enzymolysis polypeptide liquid '
6, prepared by concentrated medlar juice：Matrimony vine finished product is taken, constant temperature impregnates 30-50min, filtering in 85 DEG C of pure water, and filtrate exists
The 50-60% of pure water weight is concentrated under reduced pressure at 50-60 DEG C；
7, chrysanthemum inspissated juice extracts：Chrysanthemum tea finished product is taken, constant temperature impregnates 20-30min, filtering in 80 DEG C of pure water, and filtrate exists
The 50-60% of pure water weight is concentrated under reduced pressure at 50-60 DEG C；
8, compound is allocated
The concentrated medlar juice and step 7 prepared without Fish protein enzymolysis polypeptide liquid of the raw meat without bitter taste, step 6 prepared by step 5 is made
Standby chrysanthemum inspissated juice mixing, wherein the weight of concentrated medlar juice and chrysanthemum inspissated juice accounts for Fish protein enzymolysis polypeptide liquid weight respectively
15-30%, 3-8%；
9, mixed liquor filling and sterilization made from step 8, sealing are made.
It, can also be in the compound adaptation step of step 8 to improve the mouthfeel of enzymolysis of fish proteins polypeptide compound oral liquid
Lemon juice and white granulated sugar are added in mixed liquor, the content of wherein citric acid and white granulated sugar is respectively Fish protein enzymolysis polypeptide liquid weight
0.1-0.3%, 3-5%.
In the present embodiment, applicant also to the deodorant debitterizing method of above-mentioned offer carried out it is a variety of experiment compare, test
Process is as follows：
Method 1
Pre- deodorant step without raw material and fermentation step, Fish protein enzymolysis polypeptide liquid are only made by digesting.
Method 2
Fermentation step is omitted, only raw material passes through pre- deodorant step.
Method 3
The pre- deodorant step of raw material is omitted, i.e., only by raw material progress crushing washing without soaking in sodium carbonate solution and activated carbon adsorption
The step of.
Method 4
It is added without licorice extract in fermentation step, only adds fermented yeast and ferment.
Method 5
The complete deodorant debitterizing method recorded using the present embodiment.
Rating staff is participated in constitute：25 people of age under-18s, 18-50 Sui 30 people, 50 years old or more 15 people add up to number 70
People, sensory evaluation scores full marks 5 divide.
Test result is as follows：
| Serial number | Deodorant sensory evaluation scores | De- hardship sensory evaluation scores | Color and luster sensory evaluation scores |
| Method 1 | 2.84 | 2.65 | 4.51 |
| Method 2 | 3.86 | 3.46 | 4.48 |
| Method 3 | 4.23 | 3.81 | 4.42 |
| Method 4 | 4.56 | 3.92 | 4.34 |
| Method 5 | 4.83 | 4.68 | 4.18 |
Shown by above-mentioned test data：When the Fish protein enzymolysis polypeptide liquid prepared using method 1, since it is without the de- hardship of deodorant
Technological means, although in liquid color and luster in appearance without big influence, the bitter taste and fishy smell of product are heavier, seriously affect polypeptide liquid and exist
Application on compound oral liquid.The Fish protein enzymolysis polypeptide liquid prepared using method 2, due to being increased on the basis of method 1
The pre- deodorant means of raw material, the fishy smell and bitter taste of polypeptide liquid are declined relatively, but still are difficult to meet the mouthfeel of compound oral liquid
It is required that.The Fish protein enzymolysis polypeptide liquid prepared using method 3 increases Fishy deflavour fermentation de- bitter means on the basis of method 1, more
The fishy smell of peptide liquid is decreased obviously, but bitter taste declines deficiency, and manufactured compound oral liquid mouthfeel is still difficult to meet demand.Using side
Fish protein enzymolysis polypeptide liquid prepared by method 4 increases the pre- deodorant of raw material and Fishy deflavour fermentation de- bitter means on the basis of method 1,
The fishy smell and bitter taste of polypeptide liquid further decline, but bitter taste fall is insufficient, disclosure satisfy that the use of compound oral liquid substantially
It is required that.The Fish protein enzymolysis polypeptide liquid prepared using method 5 increases the mixing hair of licorice extract on the basis of method 4
Ferment means, the fishy smell and bitter taste of polypeptide liquid are remarkably decreased, and especially bitter taste declines apparent, although in color and luster sensory evaluation scores under
Drop, the polypeptide liquid that this method makes has slight brown stain, but can fully meet the requirement of compound oral liquid.
Above-mentioned experiment is also shown that on the de- bitter defishying to Fish protein enzymolysis polypeptide liquid, it is necessary to by pre- deodorant step,
The mixing of Radix Glycyrrhizae mixed liquor, yeast-leavened technological means, which are used in conjunction with, could meet the de- bitter requirement of its deodorant, ensure that product is final
Quality.
Experiments have shown that the addition of licorice extract has apparent improvement to polypeptide liquid fishy smell, bitter taste, especially to hardship
Taste changes significant effect, may participate in sending out due to ingredients such as glycyrrhizin, enoxolone, liquiritins in licorice extract
Maillard reaction is generated during ferment, the reaction produce fishy smell and bitter taste of the pleasant fragrance to make polypeptide liquid it is notable under
Drop makes the polypeptide liquid after fermentation generate slight brown stain as well as Maillard reaction.
In addition, applicant also compares experiment to the activation of yeast, pure water and licorice extract pair is respectively adopted
Yeast is activated, experiments have shown that, by licorice extract activate yeast fermenting speed faster, fermentation time is relative to pure
Water activation can shorten 30% or more, greatly improve the de- bitter deodorant efficiency of Fish protein enzymolysis polypeptide liquid.
It is finally bright to be：Although only providing parameter area in the present embodiment, specific parameter is not limited, such as
The parameters such as soaking time, additive amount, fermentation time, but the enlightenment of method and step that those skilled in the art provide in the embodiment
It is lower to be fully able to determine specific parameter to realize the effect reached required by the present invention by experiment.
By taking compound is allocated as an example, a kind of manufactured Fish protein enzymolysis polypeptide compound oral liquid, the compound oral liquid can be used
Following several weight proportions are made：
1, a kind of Fish protein enzymolysis polypeptide compound oral liquid, the oral solution is by Fish protein enzymolysis polypeptide liquid, concentrated medlar juice, chrysanthemum
Inspissated juice form, wherein the weight of concentrated medlar juice and chrysanthemum inspissated juice account for respectively Fish protein enzymolysis polypeptide liquid weight 15%,
8%。
2, a kind of Fish protein enzymolysis polypeptide compound oral liquid, the oral solution by Fish protein enzymolysis polypeptide liquid, concentrated medlar juice,
Chrysanthemum inspissated juice forms, and wherein the weight of concentrated medlar juice and chrysanthemum inspissated juice accounts for Fish protein enzymolysis polypeptide liquid weight respectively
30%、3%。
3, a kind of Fish protein enzymolysis polypeptide compound oral liquid, the oral solution by Fish protein enzymolysis polypeptide liquid, concentrated medlar juice,
Chrysanthemum inspissated juice forms, and wherein the weight of concentrated medlar juice and chrysanthemum inspissated juice accounts for Fish protein enzymolysis polypeptide liquid weight respectively
20%、5%。
4, a kind of Fish protein enzymolysis polypeptide compound oral liquid, the oral solution by Fish protein enzymolysis polypeptide liquid, concentrated medlar juice,
Chrysanthemum inspissated juice, citric acid, white granulated sugar composition, the wherein weight point of concentrated medlar juice, chrysanthemum inspissated juice, citric acid, white granulated sugar
18%, 6%, 0.3%, the 3% of Fish protein enzymolysis polypeptide liquid weight is not accounted for.
5, a kind of Fish protein enzymolysis polypeptide compound oral liquid, the oral solution by Fish protein enzymolysis polypeptide liquid, concentrated medlar juice,
Chrysanthemum inspissated juice, citric acid, white granulated sugar composition, the wherein weight point of concentrated medlar juice, chrysanthemum inspissated juice, citric acid, white granulated sugar
22%, 6.5%, 0.1%, the 5% of Fish protein enzymolysis polypeptide liquid weight is not accounted for.
6, a kind of Fish protein enzymolysis polypeptide compound oral liquid, the oral solution by Fish protein enzymolysis polypeptide liquid, concentrated medlar juice,
Chrysanthemum inspissated juice, citric acid, white granulated sugar composition, the wherein weight point of concentrated medlar juice, chrysanthemum inspissated juice, citric acid, white granulated sugar
24%, 3.5%, 0.3%, the 3% of Fish protein enzymolysis polypeptide liquid weight is not accounted for.
7, a kind of Fish protein enzymolysis polypeptide compound oral liquid, the oral solution by Fish protein enzymolysis polypeptide liquid, concentrated medlar juice,
Chrysanthemum inspissated juice, citric acid, white granulated sugar composition, the wherein weight point of concentrated medlar juice, chrysanthemum inspissated juice, citric acid, white granulated sugar
25.5%, 7%, 0.2%, the 4% of Fish protein enzymolysis polypeptide liquid weight is not accounted for.
Above example is only to illustrate the process technology scheme being not intended to limit the present invention, but as those skilled in the art
Member should be appreciated that：It is still possible to modify or equivalently replace the present invention, without departing from times of the scope of the present invention
What modification or localized variation, are intended to be within the scope of the claims of the invention.
Claims (8)
1. a kind of Fish protein enzymolysis polypeptide compound oral liquid, it is characterised in that：The oral solution contains Fish protein enzymolysis polypeptide liquid, Chinese holly
Qi inspissated juice, chrysanthemum inspissated juice, the wherein weight of concentrated medlar juice and chrysanthemum inspissated juice account for Fish protein enzymolysis polypeptide liquid weight respectively
15-30%, 3-8% of amount.
2. a kind of Fish protein enzymolysis polypeptide compound oral liquid according to claim 1, it is characterised in that：The Fish protein enzyme
It solves and also contains citric acid and white granulated sugar in polypeptide compound oral liquid, the content of citric acid and white granulated sugar is respectively that Fish protein enzymolysis is more
Peptide liquid 0.1-0.3%, 3-5%.
3. a kind of Fish protein enzymolysis polypeptide compound oral liquid according to claim 1, it is characterised in that：In the oral solution
Fish protein enzymolysis polypeptide liquid using de- bitter fishy smell elimination process processing, the de- bitter fishy smell elimination process adds yeast in referring to polypeptide liquid
It ferments, the fermentation includes that licorice extract is added into polypeptide liquid, by polypeptide liquid and licorice extract mixed fermentation.
4. a kind of preparation method of Fish protein enzymolysis polypeptide compound oral liquid comprising the following steps：
1）Fish protein polypeptide liquid is obtained by digesting fish raw material preparation；
2）The de- hardship of fish protein polypeptide liquid deodorant
Fish protein enzymolysis polypeptide liquid is mixed with the licorice extract of its weight 1-3%, is fermented using yeast, inoculum of dry yeast is
The 0.1-0.5% of Fish protein enzymolysis polypeptide liquid weight, 16-19 DEG C of fermentation temperature, fermentation time 3-9d, carbon dioxide pressure are kept
0.12-0.3Mpa is made without Fish protein enzymolysis polypeptide liquid of the raw meat without bitter taste；
3）It is prepared by concentrated medlar juice：Matrimony vine finished product is taken, constant temperature impregnates 30-50min, filtering in 85 DEG C of pure water, and filtrate exists
The 50-60% of pure water weight is concentrated under reduced pressure at 50-60 DEG C；
4）Chrysanthemum inspissated juice extracts：Chrysanthemum tea finished product is taken, constant temperature impregnates 20-30min, filtering in 80 DEG C of pure water, and filtrate exists
The 50-60% of pure water weight is concentrated under reduced pressure at 50-60 DEG C；
5）Compound is allocated
By step 2）Prepare without Fish protein enzymolysis polypeptide liquid of the raw meat without bitter taste, step 3）The concentrated medlar juice and step 4 of preparation）
The chrysanthemum inspissated juice of preparation mixes, and wherein the weight of concentrated medlar juice and chrysanthemum inspissated juice accounts for Fish protein enzymolysis polypeptide liquid weight respectively
15-30%, 3-8% of amount；
6）By step 5）Mixed liquor filling and sterilization obtained, sealing are made.
5. a kind of preparation method of Fish protein enzymolysis polypeptide compound oral liquid according to claim 4, it is characterised in that：On
State step 5）In compound allotment further include think that lemon juice and white granulated sugar are added in mixed liquor, wherein citric acid and white granulated sugar contains
Amount is respectively 0.1-0.3%, 3-5% of Fish protein enzymolysis polypeptide liquid weight.
6. a kind of preparation method of Fish protein enzymolysis polypeptide compound oral liquid according to claim 4, it is characterised in that：Institute
State 1）In the de- bitter step of the pre- deodorant of raw material for obtaining fish protein polypeptide liquid by digesting fish raw material preparation, further including before enzymolysis,
The step includes being impregnated 20-60 minutes in the sodium carbonate liquor for put into raw material after broken 3-5 times of raw material weight, after immersion
Raw material rinsed well using clear water, then by raw material by activated carbon filter.
7. a kind of preparation method of Fish protein enzymolysis polypeptide compound oral liquid according to claim 4, it is characterised in that：Institute
State 2）The de- hardship of fish protein polypeptide liquid deodorant, further includes the activation of yeast, which is：Radix Glycyrrhizae is put into 50-60 times of its weight
In the water of weight, water temperature controls 70-80 DEG C, decocts to carry 20-40 minutes and filter, filtered fluid heating is concentrated into the 10- of Radix Glycyrrhizae weight
20 times obtain licorice extract, and Fish protein, the licorice extract heating of enzymolysis polypeptide liquid weight 1-3% is taken to boil postcooling to 30-35
DEG C, yeast is added and is activated, inoculum of dry yeast is the 0.1-0.5% of Fish protein enzymolysis polypeptide liquid weight.
8. a kind of preparation method of Fish protein enzymolysis polypeptide compound oral liquid according to claim 4, it is characterised in that：Institute
State step 1）Fish protein polypeptide liquid is obtained by digesting fish raw material preparation, enzymolysis step is as follows：
1）Raw material is put into the pure water of 2.5-5 times of its weight, adds animal protease and papain, additive amount are
The 2 ‰ -4 ‰ of raw material, 3.5-4.5h is digested at 51-55 DEG C, filters out the residue fish-skin slag for not completing enzymolysis；
2）It will be through step 1）Obtained enzymolysis liquid boils rapidly, and after keeping 1-2min, is cooled to room temperature with tap water water-bath；
3）By step 2）Solution at 2-4 DEG C, in separatory funnel stand 8-12h, after being layered, from separatory funnel lower end be discharged
Outstanding heavy layer, obtains clarified solution；
4）It is concentrated under reduced pressure：By step 3）30- is concentrated under reduced pressure at 55-60 DEG C, vacuum degree 0.09-0.10Mpa in obtained clarified solution
60min。
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