CN108288578A - Paper substrate sampling device and method - Google Patents
Paper substrate sampling device and method Download PDFInfo
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- CN108288578A CN108288578A CN201711493775.7A CN201711493775A CN108288578A CN 108288578 A CN108288578 A CN 108288578A CN 201711493775 A CN201711493775 A CN 201711493775A CN 108288578 A CN108288578 A CN 108288578A
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- detection zone
- paper substrate
- sample
- zone
- sampling device
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
- H01J49/0409—Sample holders or containers
Abstract
The present invention provides a kind of paper substrate sampling device and method, the paper substrate sampling device includes ion source, filter paper and mass spectrum injection port；Rotating platform, the filter paper are arranged on the rotating platform, and are rotated around shaft under driving；The filter paper has hydrophobic region and hydrophilic area, the hydrophilic area includes sample application zone, detection zone and interface channel, and the sample application zone is in the center of the filter paper, and the detection zone is at least two, and be distributed around the sample application zone, the interface channel connects the sample application zone and detection zone；When the filter paper rotates, the line between the ion source and mass spectrum injection port passes through the detection zone.The present invention has many advantages, such as sensitivity for analysis height, high throughput analysis.
Description
Technical field
The present invention relates to mass spectral analyses, more particularly to paper substrate sampling device and method.
Background technology
Open type analytical technique of mass spectrum, can be under atmospheric pressure open environment, extremely short without complicated sample pre-treatments
The in situ, real-time of sample, fast ionic are realized in time, with portable devices, testing cost it is low, applied widely etc.
Advantage has wide application in various fields such as food inspection, Pharmaceutical Analysis, environmental monitoring, public safety and clinical diagnosises
Foreground.
Filter paper is since its is at low cost, portable, and unique advantage such as energy function dough, by as a kind of novel sample introduction
Mode is applied in open type mass spectral analysis.And filter paper input mode is used, capillary and glass bar etc. can be made up and be difficult to
It realizes the defect of large biological molecule sample analysis, realizes the efficient analysis of the macromolecular substances such as polypeptide, protein.Not filter paper
In the presence of the energy that can greatly weaken open type mass ion source institute jet plasma beam so that (especially small to sample segment
Molecular sample) ionizability weaken, so as to cause sample detection signal decline.In addition, previously which can only use
Hand sampling, there are larger interference from human factor, cannot achieve sample automation, high throughput analysis.
It is above-mentioned that following defect is primarily present based on filter paper input mode：
1. based on the weak energy for weakening open type mass ion source institute jet plasma beam of paper substrate input mode meeting, lead
Sample detector signal is caused to decline.
2. traditional paper substrate is using manually input mode, there are larger interference from human factor, cannot achieve sample certainly
Dynamicization, high throughput analysis.
Invention content
For the deficiency for solving in above-mentioned prior art, that the present invention provides a kind of sensitivity for analysis is high, accuracy is high,
The paper substrate sampling device of high throughput analysis.
The purpose of the present invention is what is be achieved through the following technical solutions：
Paper substrate sampling device, the paper substrate sampling device include ion source, filter paper and mass spectrum injection port；The paper substrate sample introduction
Device further comprises：
Rotating platform, the filter paper are arranged on the rotating platform, and are rotated around shaft under driving；
It includes sample application zone, detection zone and interface channel that the filter paper, which has hydrophobic region and hydrophilic area, the hydrophilic area, described
Sample application zone is in the center of the filter paper, and the detection zone is at least two, and is distributed around the sample application zone, and the connection is logical
Road connects the sample application zone and detection zone；When the filter paper rotates, the line between the ion source and mass spectrum injection port is worn
Cross the detection zone.
The present invention also aims to provide the high paper substrate of with high accuracy, high throughput analysis, sensitivity for analysis into
Quadrat method, the goal of the invention technical scheme is that：
Paper substrate sample injection method, the paper substrate sample injection method include the following steps：
(A1) testing sample solution is added dropwise in detection zone, and filter paper is fixed on rotating platform, filter paper is driving later
Under rotate around the shaft；
(A2) action of plasma that ion source projects makes the sample to be tested of detection zone distinguish ion in different detection zones
Change, enters mass spectrum injection port to the sample timesharing of ionization.
Compared with prior art, the device have the advantages that being：
1. accurately controlling sample introduction by rotating platform, interference caused by eliminate human factor improves precision of analysis,
Realize automation and the high throughput analysis of sample；
2. can effectively improve Mass Spectrometer Method signal by adding gold nano grain in detection zone, sensitivity for analysis is promoted；
3. the unique filter paper hydrophilic pattern of design, realizes that multiple detection zones are nano-particle modified simultaneously through capillary action；
4. device is simple, easy to operate, detection efficiency can be promoted.
Description of the drawings
With reference to attached drawing, the disclosure will be easier to understand.Skilled addressee readily understands that be：This
A little attached drawings are used only for the technical solution illustrated the present invention, and are not intended to and are construed as limiting to protection scope of the present invention.
In figure：
Fig. 1 is the structure diagram of paper substrate sampling device according to the ... of the embodiment of the present invention；
Fig. 2 is the structure diagram of filter paper according to the ... of the embodiment of the present invention；
Fig. 3 is the according to embodiments of the present invention 2 corresponding mass spectrogram of colistine sulfate；
Fig. 4 is the according to embodiments of the present invention 3 corresponding mass spectrogram of crystal methamphetamine.
Specific implementation mode
Fig. 1-4 and following description describe the present invention optional embodiment to instruct how those skilled in the art implement
It is of the invention with reproducing.In order to instruct technical solution of the present invention, some conventional aspects are simplified or have been omitted.Those skilled in the art
It should be appreciated that modification or replacement from these embodiments will within the scope of the invention.Those skilled in the art should understand that
Following characteristics can be combined in various ways to form multiple modifications of the present invention.As a result, the invention is not limited in it is following can
Embodiment is selected, and is only limited by the claims and their equivalents.
Embodiment：
Fig. 1 schematically illustrates the structure diagram of the paper substrate sampling device of the embodiment of the present invention 1, as shown in Figure 1, described
Paper substrate sampling device includes：
Ion source, filter paper and mass spectrum injection port；It is characterized in that：The paper substrate sampling device further comprises：
Rotating platform, the filter paper are arranged on the rotating platform, and are rotated around shaft under driving；The rotation
Platform includes：
Fixed frame, the filter paper are arranged on fixed frame；When the line passes through the detection zone, not by the fixation
The blocking of frame；
Drive module, the drive module is for driving the fixed frame to rotate so that the line passes through detection zone；
It includes sample application zone 3, detection zone 2 and interface channel 4 that the filter paper, which has hydrophobic region 1 and hydrophilic area, the hydrophilic area,
The sample application zone is in the center of the filter paper, and the detection zone is at least two, and is distributed around the sample application zone, the company
It connects road and connects the sample application zone and detection zone；The detection zone and sample application zone are circle：The center of the detection zone is in same
On one circle, the center of circle of the circle is the center of circle of the sample application zone；A diameter of 2-4mm of the sample application zone, the detection zone it is straight
Diameter is 2-3mm；The width of the interface channel is less than the diameter of the detection zone and sample application zone；When the filter paper rotates, institute
The line between ion source and mass spectrum injection port is stated perpendicular to the filter paper and passes through the detection zone, the center of circle of the detection zone
On the line.
The course of work of the paper substrate sample injection method of the embodiment of the present invention namely above-mentioned paper substrate sampling device, the paper substrate into
Quadrat method includes the following steps：
(A0) gold nano grain solution is added dropwise in sample application zone, gold nano grain passes through interface channel to enter through capillary action
The detection zone；
(A1) testing sample solution is added dropwise in detection zone, and filter paper is fixed on rotating platform, filter paper is driving later
Under rotate around the shaft；
(A2) action of plasma that ion source projects makes the sample to be tested of detection zone distinguish ion in different detection zones
Change, enters mass spectrum injection port to the sample timesharing of ionization.
Embodiment 2：
Application examples of the according to embodiments of the present invention 1 paper substrate sampling device and method in colistine sulfate detection.
In the application examples, detection zone is 8, and the center of adjacent detection zone is between the connecting line at the center of the sample application zone
Angle be 45 degree；It is diameter 4mm round to be loaded district center, and interface channel length is 15mm, width 1mm；Detection zone is straight
Diameter is 3mm round；Dropwise addition gold nano grain solution is 20 μ l；Dropwise addition testing sample solution is 3 μ l；Filter paper is apart from mass spectrum injection port
Distance is 0.2mm, and ion source nozzle, the detection zone center of circle and mass spectrum injection port are located in same horizontal line；Mass Spectrometer Method m/z ranges
It is set as 200-1200, detection sample is that (solvent is methanol to 10ppm colistine sulfates standard sample：Water=1:1 mixed solution),
Corresponding mass spectrogram is as shown in Figure 3.1156.69 (one charge of band) and 578.65 (two charges of band) two therefrom can be observed
A echo signal peak, intensity are respectively 1.85e4And 4.95e3, signal-to-noise ratio is 21.45 and 10.88.Gained after moving in rotation platform
Four measured value relative standard deviations are 7.23%.
Embodiment 3：
Application examples of the according to embodiments of the present invention 1 paper substrate sampling device and method in crystal methamphetamine detection.
In the application examples, detection zone is 8, and the center of adjacent detection zone is between the connecting line at the center of the sample application zone
Angle be 45 degree；Sample application zone is diameter 3mm round, and interface channel length is 15mm, width 1mm；Detection zone is a diameter of
2mm is round；Dropwise addition gold nano grain solution is 15 μ l；Dropwise addition testing sample solution is 3 μ l；Filter paper is apart from mass spectrum injection port distance
For 0.2mm, ion source nozzle, the detection zone center of circle and mass spectrum injection port are located in same horizontal line.Mass Spectrometer Method m/z ranges are set as
60-400, detection sample is that (solvent is methanol to 10ppb crystal methamphetamines sample：Water=1:1 mixed solution), corresponding mass spectrum spectrum
Figure is as shown in Figure 4.Apparent sample therefrom can be observed and add hydrogen peak, intensity 7.88e2, signal-to-noise ratio 16.3.Moving in rotation platform
Four measured value relative standard deviations of gained are 12.52% afterwards.
Claims (10)
1. paper substrate sampling device, the paper substrate sampling device includes ion source, filter paper and mass spectrum injection port；It is characterized in that：Institute
Paper substrate sampling device is stated to further comprise：
Rotating platform, the filter paper are arranged on the rotating platform, and are rotated around shaft under driving；
The filter paper has hydrophobic region and hydrophilic area, and the hydrophilic area includes sample application zone, detection zone and interface channel, the sample-adding
Area is in the center of the filter paper, and the detection zone is at least two, and is distributed around the sample application zone, and the interface channel connects
Connect the sample application zone and detection zone；When the filter paper rotates, the line between the ion source and mass spectrum injection port passes through institute
State detection zone.
2. paper substrate sampling device according to claim 1, it is characterised in that：The detection zone and sample application zone are circle, institute
The width for stating interface channel is less than the diameter of the detection zone and sample application zone.
3. paper substrate sampling device according to claim 2, it is characterised in that：The center of the detection zone is in the same circle
On, the center of circle of the circle is the center of circle of the sample application zone.
4. paper substrate sampling device according to claim 2, it is characterised in that：A diameter of 2-4mm of the sample application zone, it is described
A diameter of 2-3mm of detection zone.
5. paper substrate sampling device according to claim 2, it is characterised in that：The line is perpendicular to the filter paper, and institute
The center of circle for stating detection zone is on the line.
6. paper substrate sampling device according to claim 1, it is characterised in that：The rotating platform includes：
Fixed frame, the filter paper are arranged on fixed frame；When the line passes through the detection zone, not by the fixed frame
Blocking；
Drive module, the drive module is for driving the fixed frame to rotate so that the line passes through detection zone.
7. paper substrate sampling device according to claim 1, it is characterised in that：The center of adjacent detection zone to the sample application zone
Center connecting line between angle be 45 degree.
8. paper substrate sampling device according to claim 1, it is characterised in that：The detection zone has gold nano grain.
9. according to the paper substrate sample injection method of any paper substrate sampling devices of claim 1-7, the paper substrate sample injection method packet
Include following steps：
(A1) testing sample solution is added dropwise in detection zone, and filter paper is fixed on rotating platform, later filter paper under driving around
Shaft rotates；
(A2) action of plasma that ion source projects makes the sample to be tested of detection zone ionize respectively in different detection zones,
Enter mass spectrum injection port to the sample timesharing of ionization.
10. paper substrate sample injection method according to claim 9, it is characterised in that：The paper substrate sample injection method further comprises
Following steps：
(A0) gold nano grain solution is added dropwise in sample application zone, gold nano grain enters the detection zone by interface channel.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711493775.7A CN108288578B (en) | 2017-12-31 | 2017-12-31 | Paper-based sample injection device and method |
| PCT/CN2018/125474 WO2019129269A1 (en) | 2017-12-31 | 2018-12-29 | Paper-based sample feeding device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711493775.7A CN108288578B (en) | 2017-12-31 | 2017-12-31 | Paper-based sample injection device and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108288578A true CN108288578A (en) | 2018-07-17 |
| CN108288578B CN108288578B (en) | 2020-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711493775.7A Active CN108288578B (en) | 2017-12-31 | 2017-12-31 | Paper-based sample injection device and method |
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| CN (1) | CN108288578B (en) |
| WO (1) | WO2019129269A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019129269A1 (en) * | 2017-12-31 | 2019-07-04 | 宁波大学 | Paper-based sample feeding device and method |
| CN114078687A (en) * | 2020-08-20 | 2022-02-22 | 中国科学院化学研究所 | Capillary paper spray ion source device and ion generation method |
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| CN102539515A (en) * | 2011-12-27 | 2012-07-04 | 北京大学 | High-sensitivity detection method of normal temperature normal pressure surface assisted laser desorption mass spectrometry |
| CN104946505A (en) * | 2014-03-24 | 2015-09-30 | 中国科学院深圳先进技术研究院 | Microfluidic chip for realizing PCR (polymerase chain reaction) and virus rapid detection device for real-time PCR |
| WO2016130646A1 (en) * | 2015-02-10 | 2016-08-18 | Indiana University Research And Technology Corporation | Device and method for analysis of biofluids by ion generation using wetted porous material |
| CN205844190U (en) * | 2016-07-18 | 2016-12-28 | 浙江华久生物技术有限公司 | A kind of paper substrate micro fluidic device |
| CN106960777A (en) * | 2016-12-31 | 2017-07-18 | 宁波华仪宁创智能科技有限公司 | Mass spectrometry system and its method of work |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108288578B (en) * | 2017-12-31 | 2020-08-11 | 宁波大学 | Paper-based sample injection device and method |
| CN207624658U (en) * | 2017-12-31 | 2018-07-17 | 宁波大学 | Paper substrate sampling device for mass spectral analysis |
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2017
- 2017-12-31 CN CN201711493775.7A patent/CN108288578B/en active Active
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2018
- 2018-12-29 WO PCT/CN2018/125474 patent/WO2019129269A1/en active Application Filing
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| CN102539515A (en) * | 2011-12-27 | 2012-07-04 | 北京大学 | High-sensitivity detection method of normal temperature normal pressure surface assisted laser desorption mass spectrometry |
| CN104946505A (en) * | 2014-03-24 | 2015-09-30 | 中国科学院深圳先进技术研究院 | Microfluidic chip for realizing PCR (polymerase chain reaction) and virus rapid detection device for real-time PCR |
| WO2016130646A1 (en) * | 2015-02-10 | 2016-08-18 | Indiana University Research And Technology Corporation | Device and method for analysis of biofluids by ion generation using wetted porous material |
| CN205844190U (en) * | 2016-07-18 | 2016-12-28 | 浙江华久生物技术有限公司 | A kind of paper substrate micro fluidic device |
| CN106960777A (en) * | 2016-12-31 | 2017-07-18 | 宁波华仪宁创智能科技有限公司 | Mass spectrometry system and its method of work |
Non-Patent Citations (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019129269A1 (en) * | 2017-12-31 | 2019-07-04 | 宁波大学 | Paper-based sample feeding device and method |
| CN114078687A (en) * | 2020-08-20 | 2022-02-22 | 中国科学院化学研究所 | Capillary paper spray ion source device and ion generation method |
| CN114078687B (en) * | 2020-08-20 | 2023-03-21 | 中国科学院化学研究所 | Capillary paper spray ion source device and ion generation method |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019129269A1 (en) | 2019-07-04 |
| CN108288578B (en) | 2020-08-11 |
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