CN108283283A - A kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin - Google Patents
A kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin Download PDFInfo
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- CN108283283A CN108283283A CN201711487701.2A CN201711487701A CN108283283A CN 108283283 A CN108283283 A CN 108283283A CN 201711487701 A CN201711487701 A CN 201711487701A CN 108283283 A CN108283283 A CN 108283283A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/273—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption using adsorption or absorption agents, resins, synthetic polymers, or ion exchangers
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
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Abstract
The present invention relates to technical field of food safety, a kind of methods for ciguatoxin of degrading in particular to electron beam irradiation and chitosan microball interaction.A kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin, includes the following steps：（1）Marine product containing ciguatoxin is subjected to pre-treatment；（2）Marine product Jing Guo pre-treatment is impregnated jointly with chitosan microball, it is 6 ~ 8 then to adjust the pH of soak by acetic acid solution and sodium hydroxide solution, and it is 13 ~ 20 DEG C to keep the temperature of soak, is adsorbed；（3）It will be positioned under electron beam irradiation light by the processed marine product of chitosan microball and carry out electron beam irradiation.There is the present invention a kind for the treatment of effeciency height, the good advantage of effect to help to solve harm of the ciguatoxin to Food industry.
Description
Technical field
The present invention relates to technical field of food safety, in particular to a kind of electron beam irradiation and chitosan microball interaction
The method of degradation ciguatoxin.
Background technology
The name of ciguatoxin is from snow card fish, the toxin once isolated mistake from more than 400 kinds of fishes, but it is really
Source is a kind of Dinoflagellate hilllock than malicious dinoflagellate（.Ciguatoxin is a kind of fat-soluble high ether material, and toxicity is very strong, compares filefish
Toxin is 100 times strong, is more serious one of the red tide plankton toxin of known harmfulness, and colorless and odorless is fat-soluble, not soluble in water,
It is heat-resisting, it is not easy to be destroyed by hydrochloric acid in gastric juice, be primarily present in the internal organ of julian celtis, muscle, be especially height with content in internal organ.Have found 3
Class ciguatoxin, i.e. Pacific Ocean ciguatoxin, Caribbean ciguatoxin and India's ciguatoxin.No matter quantitatively or
In toxicity, ciguatoxin is known to one of strongest toxin of mammalian toxicity.
Ciguatoxin is endangered mainly caused by human body by the edible herbivorous fishes containing ciguatoxin and carnivorous fish
Caused by class.So far from the 1980s, the increase with the mankind to marine protein dependence, worldwide puts down
Etesian Ciguatoxin Poisoning number is up to people more than 50,000.However, evidence suggests the migratory and fish productions due to fish
The trade of product expands, and ciguatoxin can also influence other areas.The all islands of South China Sea, the Taiwan Straits and Hongkong often have
Ciguatoxin Poisoning event occurs.Ciguatoxin, which has become, influences fishery economic development and sanitarian one big obstacle.
Chitosan is that the chitin being widely present by nature hydrolyzes and sloughs life after the acetyl group of part under alkaline condition
At derivative, abundance is nontoxic.From 1859, after Frenchman Rouget obtains chitosan first, this natural polymer
Biological functionality and the excellent performances such as compatibility, blood compatibility, safety, microbic resolvability closed extensively by all trades and professions
Note, in the numerous areas such as medicine, food, chemical industry, cosmetics, water process, METAL EXTRACTION and recycling, biochemistry and biomedical engineering
Application study achieve major progress.
The chitosan of native state is largely powdered, as absorption carrier, carrier and adsorbate is made all to be difficult to recycle,
To limit its application.Under given conditions, hydrolysis, alkylation, acylation, carboxy methylation, sulfonation, nitre can occur for chitosan
The chemical reactions such as change, halogenation, oxidation, reduction, condensation and complexing produce the various chitosan derivatives with different performance,
To expand the application range of chitosan.
Chitosan can be all applied in protein-interacting, antigen-antibody reaction, the diagnosis of relevant disease etc., specifically
In proteopexy to microballoon, then to carry out correlation analysis or detection.Currently, mostly using epoxy or-CN groups in the market
As activated group.Because the purposes of different activation methods is different, developing a variety of different activation methods could meet
The actual demand of scientific research.
And based on currently existing electron beam irradiation processing technology, with x-ray, gamma-rays or high-velocity electron beam etc.
The high-energy ray that the irradiation of ionization electron beam generates is processed food, reaches desinsection, sterilization, inhibits physiology course, carries
High food hygiene quality keeps nutritional quality and the purpose of flavor, Shelf-life.And irradiating also has some biotoxins
Degradation.
Therefore in order to solve harm of the biotoxin for Food industry, need a kind for the treatment of effeciency high, effect is good
Electron beam irradiation and chitosan microball interaction degradation ciguatoxin method.
Invention content
To solve the above-mentioned problems, a kind of electron beam irradiation is provided and prepares chitosan microball for adsorbing biotoxin
Method, the present invention use following technical scheme：
A kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin, includes the following steps：
（1）Marine product containing ciguatoxin is subjected to pre-treatment；
（2）Marine product Jing Guo pre-treatment is impregnated jointly with chitosan microball, it is then molten by acetic acid solution and sodium hydroxide
The pH that liquid adjusts soak is 6 ~ 8, and it is 13 ~ 20 DEG C to keep the temperature of soak, is adsorbed.
（3）It will be positioned under electron beam irradiation light by the processed marine product of chitosan microball and carry out electron beam irradiation.
The present invention carries out pre-treatment work to the marine product containing ciguatoxin first, then by chitosan microball to containing
There is the marine product of ciguatoxin to carry out adsorption treatment, by still having some ciguatoxin in chitosan treated marine product
Residual, is irradiated at this time by using electron beam, again at this time since the effect of electron beam irradiation makes ciguatoxin have occurred
Cracking, first lose-H2O loses C=O bond again, to further decrease the content of ciguatoxin, in reducing
Point danger of poison.
Preferably, the pre-treatment includes the following steps：Marine product containing ciguatoxin is carried out with tap water
Cleaning, drains, and it is marinated that sodium carbonate liquor is then added.
The marine product containing ciguatoxin is pickled under weak basic condition in the present invention, since weak base condition can
So that ciguatoxin is degraded, to reduce toxic effect, safety is improved.
Preferably, the marinated temperature is at 4 ~ 5 DEG C, the marinated time is 3 ~ 5h, the sodium carbonate liquor
Mass fraction be 0.3 ~ 1.5%.
Using 0.3% ~ 1.5% concentration range sodium carbonate liquor concentration can keep marine product especially avenge be stuck in not by
Play the role of ciguatoxin of degrading in the case of destroying its flavor and nutritive value.
Preferably, the chitosan microball is prepared using following methods：
Gradually 120 ~ 160 parts by weight acetic acid solutions are added in 3 ~ 4 parts by weight chitosans first, with the rate of 200 ~ 300r/min
Stir 30 ~ 35min so that chitosan is dissolved in acetic acid solution, and then deepfreeze 18 ~ 20 hours, then inhale chitosan
Enter in syringe, then the chitosan in syringe is added dropwise in condensation water, band chitosan is spent after being condensed into sphere
Ionized water is washed repeatedly as neutrality, is then dried.
Lytic agent of the acetic acid as chitosan has been used in the present invention, since the glycosidic bond of glycan is hemiacetal structure,
And this hemiacetal structure is unstable to acid, acid catalyzed hydrolysis can occur in placement process for the acid solution of chitosan
Reaction prevents acidity is too strong from leading to degradation of chitosan using acetic acid, and the faintly acid of acetic acid can then improve the dispersion of chitosan
Ability contributes to the preparation of chitosan microball.And the mode for using deepfreeze can reduce the palliating degradation degree of chitosan, carry
The function and effect of high chitosan microball.
Preferably, the condensation water is prepared using following methods：It is added 2 ~ 3 in the sodium hydroxide of 40 ~ 50 parts by weight
The ethyl acetate of parts by weight, uniform stirring.
The present invention can form the higher solution of pH value using ethyl acetate and sodium hydroxide as coagulating agent, help to carry
The efficiency of high chitosan balling-up, while the sodium hydroxide of this kind of ratio of use and ethyl acetate mixing, since ethyl acetate is small
The compound of molecular weight can reduce in sodium hydroxide, and chitosan microball table can be improved using the mixed liquor of this kind of ratio
The compactness extent in face is conducive to the adsorption capacity for improving chitosan microball for ciguatoxin.
Preferably, the chitosan is prepared by following methods：
（1）The shrimp shell of 5 ~ 6 parts by weight is put in 3 ~ 4h of immersion in the hydrochloric acid of the 1mol/L of 600 ~ 700 parts by weight, then by shrimp shell
It is put into after taking-up in the hydrochloric acid of 1000 ~ 1200 parts by weight of same concentration and continues 8 ~ 9h of immersion, in 500 ~ 550 weight after then taking out
40 ~ 100min is boiled in part 3% sodium hydroxide solution, bleaching is dried under daylight again after solid is taken out, obtains chitin；
（2）Then chitin is immersed in the sodium hydroxide that mass fraction is 40 ~ 50%, 2 ~ 3h is kept the temperature at 90 ~ 95 DEG C.
It adopts this method in the present invention and is is prepared by chitosan, use shrimp shell first as raw material, reduce
The manufacturing cost of chitosan, and the pollution for environment is reduced while reusing scrap feed material, and the present invention
The method for carrying out soda boiling again by first acid soak can reduce the ash content of obtained chitin, to improve obtained chitin
The surface soundness of microballoon to chitosan microball processing capacity, and use below higher concentration sodium hydroxide and compared with
High treatment temperature can obtain the higher chitin of de- second phthalein degree, to improve the adsorption capacity of chitin.The present invention also adopts
With the method for substep pickling and soda boiling, helps to reduce the impurity composition in chitin, improve chitosan part adsorption capacity.
Preferably, the step（2）In adsorption process be concussion adsorb.
The present invention can enhance suction-operated of the chitosan microball to ciguatoxin using the method for concussion absorption, increase and inhale
Attached efficiency.
Preferably, the concussion adsorption process is that whole system is fast with the concussion of 100 ~ 120r/min on shaking table
Rate is shaken, and the time for shaking absorption is 3 ~ 5h.
It can be reduced while improving adsorption efficiency due to excessively quickly shake using the concussion rate and concussion time
It swings and the possibility destroyed is generated to the structure of chitosan microball, ensure that the function and effect of chitosan microball.
Preferably, the dosage of the electron beam irradiation is 5 ~ 10kGy.
Present invention employs the dose of radiations, and degradation ciguatoxin can be played in the case where meeting food hygiene and safety
Effect.
Preferably, the electron beam irradiation uses dosage for 10 kGy.
When the irradiation of food not will produce toxicological effect then in 10 kGy or less, i.e., irradiation descends food not at this dose
Meeting do harm to huamn body, and the dosage can play ciguatoxin good degradation, and the degree of degradation can reach
To 30% or more.Have and obviously acts on.
The beneficial effects of the present invention are：The present invention has, good degrading effect and right high to ciguatoxin treatment effeciency
Human body not will produce the advantage of new toxicological effect.
Specific implementation mode
The present invention is further explained with reference to specific implementation case：
Embodiment 1
A kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin, includes the following steps：
（1）Marine product containing ciguatoxin is cleaned with tap water, is drained, sodium carbonate liquor is then added and pickles, institute
The marinated temperature stated is at 4 DEG C, and the marinated time is 3h, and the mass fraction of the sodium carbonate liquor is 1%；
（2）It will jointly be impregnated with chitosan microball by marinated marine product, then pass through acetic acid solution and sodium hydroxide solution
The pH for adjusting soak is 6, and it is 13 DEG C to keep the temperature of soak, carries out concussion absorption, the concussion adsorption process is will
Whole system is shaken on shaking table with the concussion rate of 100r/min, and the time for shaking absorption is 3h.
（3）It will be positioned under electron beam irradiation light by the processed marine product of chitosan microball and carry out electron beam irradiation,
The dosage of the electron beam irradiation light is 10kGy.
Wherein, the chitosan microball is prepared using following methods：
Gradually 120 parts by weight acetic acid solutions are added in 3 parts by weight chitosans first, 30min is stirred with the rate of 200r/min,
So that chitosan is dissolved in acetic acid solution, then deepfreeze 18 hours then will then by chitosan inhalation syringe
Chitosan in syringe is added dropwise in condensation water, band chitosan be condensed into after sphere washed repeatedly with deionized water as
Then neutrality is dried, wherein the condensation water is prepared using following methods：It is added 2 in the sodium hydroxide of 40 parts by weight
The ethyl acetate of parts by weight, uniform stirring.
Wherein, the chitosan is prepared by following methods：
（1）The shrimp shell of 5 parts by weight is put in the hydrochloric acid of the 1mol/L of 600 parts by weight and impregnates 4h, put after then taking out shrimp shell
Enter and continue to impregnate 9h in the hydrochloric acid of 1000 parts by weight of same concentration, in the sodium hydroxide solution of 500 parts by weight 3% after then taking out
In boil 40min, dry bleaching under daylight again after solid is taken out, obtain chitin；
（2）Then chitin is immersed in the sodium hydroxide that mass fraction is 40%, 2h is kept the temperature at 90 DEG C.
Embodiment 2
A kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin, includes the following steps：
（1）Marine product containing ciguatoxin is cleaned with tap water, is drained, sodium carbonate liquor is then added and pickles, institute
The marinated temperature stated is at 5 DEG C, and the marinated time is 5h, and the mass fraction of the sodium carbonate liquor is 1.5%；
（2）It will jointly be impregnated with chitosan microball by marinated marine product, then pass through acetic acid solution and sodium hydroxide solution
The pH for adjusting soak is 8, and it is 20 DEG C to keep the temperature of soak, carries out concussion absorption, the concussion adsorption process is will
Whole system is shaken on shaking table with the concussion rate of 120r/min, and the time for shaking absorption is 5h.
（3）It will be positioned under electron beam irradiation light by the processed marine product of chitosan microball and carry out electron beam irradiation,
The dosage of the electron beam irradiation light is 5kGy.
Wherein, the chitosan microball is prepared using following methods：
Gradually 160 parts by weight acetic acid solutions are added in 3 parts by weight chitosans first, 35min is stirred with the rate of 300r/min,
So that chitosan is dissolved in acetic acid solution, then deepfreeze 20 hours then will then by chitosan inhalation syringe
Chitosan in syringe is added dropwise in condensation water, band chitosan be condensed into after sphere washed repeatedly with deionized water as
Then neutrality is dried, wherein the condensation water is prepared using following methods：It is added 3 in the sodium hydroxide of 50 parts by weight
The ethyl acetate of parts by weight, uniform stirring.
Wherein, the chitosan is prepared by following methods：
（1）The shrimp shell of 6 parts by weight is put in the hydrochloric acid of the 1mol/L of 700 parts by weight and impregnates 4h, put after then taking out shrimp shell
Enter and continue to impregnate 9h in the hydrochloric acid of 1200 parts by weight of same concentration, in the sodium hydroxide solution of 550 parts by weight 3% after then taking out
In boil 100min, dry bleaching under daylight again after solid is taken out, obtain chitin；
（2）Then chitin is immersed in the sodium hydroxide that mass fraction is 50%, 3h is kept the temperature at 95 DEG C.
Embodiment 3
A kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin, includes the following steps：
（1）Marine product containing ciguatoxin is cleaned with tap water, is drained, sodium carbonate liquor is then added and pickles, institute
The marinated temperature stated is at 5 DEG C, and the marinated time is 5h, and the mass fraction of the sodium carbonate liquor is 0.6%；
（2）It will jointly be impregnated with chitosan microball by marinated marine product, then pass through acetic acid solution and sodium hydroxide solution
The pH for adjusting soak is 7, and it is 15 DEG C to keep the temperature of soak, carries out concussion absorption, the concussion adsorption process is will
Whole system is shaken on shaking table with the concussion rate of 100/min, and the time for shaking absorption is 4h.
（3）It will be positioned under electron beam irradiation light by the processed marine product of chitosan microball and carry out electron beam irradiation,
The dosage of the electron beam irradiation light is 10kGy.
Wherein, the chitosan microball is prepared using following methods：
Gradually 140 parts by weight acetic acid solutions are added in 4 parts by weight chitosans first, 30min is stirred with the rate of 200r/min,
So that chitosan is dissolved in acetic acid solution, then deepfreeze 18 hours then will then by chitosan inhalation syringe
Chitosan in syringe is added dropwise in condensation water, band chitosan be condensed into after sphere washed repeatedly with deionized water as
Then neutrality is dried, wherein the condensation water is prepared using following methods：It is added 2 in the sodium hydroxide of 45 parts by weight
The ethyl acetate of parts by weight, uniform stirring.
Wherein, the chitosan is prepared by following methods：
（1）The shrimp shell of 5 parts by weight is put in the hydrochloric acid of the 1mol/L of 600 parts by weight and impregnates 3h, put after then taking out shrimp shell
Enter and continue to impregnate 9h in the hydrochloric acid of 1000 parts by weight of same concentration, in the sodium hydroxide solution of 500 parts by weight 3% after then taking out
In boil 60min, dry bleaching under daylight again after solid is taken out, obtain chitin；
（2）Then chitin is immersed in the sodium hydroxide that mass fraction is 45%, 3h is kept the temperature at 95 DEG C.
Embodiment 4
A kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin, includes the following steps：
（1）Marine product containing ciguatoxin is cleaned with tap water, is drained, sodium carbonate liquor is then added and pickles, institute
The marinated temperature stated is at 5 DEG C, and the marinated time is 5h, and the mass fraction of the sodium carbonate liquor is 1.5%；
（2）It will jointly be impregnated with chitosan microball by marinated marine product, then pass through acetic acid solution and sodium hydroxide solution
The pH for adjusting soak is 7, and it is 20 DEG C to keep the temperature of soak, carries out concussion absorption, the concussion adsorption process is will
Whole system is shaken on shaking table with the concussion rate of 100r/min, and the time for shaking absorption is 5h.
（3）It will be positioned under electron beam irradiation light by the processed marine product of chitosan microball and carry out electron beam irradiation,
The dosage of the electron beam irradiation light is 10kGy.
Wherein, the chitosan microball is prepared using following methods：
Gradually 140 parts by weight acetic acid solutions are added in 3 parts by weight chitosans first, 30min is stirred with the rate of 200r/min,
So that chitosan is dissolved in acetic acid solution, then deepfreeze 18 hours then will then by chitosan inhalation syringe
Chitosan in syringe is added dropwise in condensation water, band chitosan be condensed into after sphere washed repeatedly with deionized water as
Then neutrality is dried, wherein the condensation water is prepared using following methods：It is added 2 in the sodium hydroxide of 45 parts by weight
The ethyl acetate of parts by weight, uniform stirring.
Wherein, the chitosan is prepared by following methods：
（1）The shrimp shell of 5 parts by weight is put in the hydrochloric acid of the 1mol/L of 600 parts by weight and impregnates 3h, put after then taking out shrimp shell
Enter and continue to impregnate 9h in the hydrochloric acid of 1000 parts by weight of same concentration, in the sodium hydroxide solution of 500 parts by weight 3% after then taking out
In boil 60min, dry bleaching under daylight again after solid is taken out, obtain chitin；
（2）Then chitin is immersed in the sodium hydroxide that mass fraction is 45%, 3h is kept the temperature at 95 DEG C.
Embodiment 5
A kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin, includes the following steps：
（1）Marine product containing ciguatoxin is cleaned with tap water, is drained, sodium carbonate liquor is then added and pickles, institute
The marinated temperature stated is at 4 DEG C, and the marinated time is 3h, and the mass fraction of the sodium carbonate liquor is 1.5%；
（2）It will jointly be impregnated with chitosan microball by marinated marine product, then pass through acetic acid solution and sodium hydroxide solution
The pH for adjusting soak is 7, and it is 20 DEG C to keep the temperature of soak, carries out concussion absorption, the concussion adsorption process is will
Whole system is shaken on shaking table with the concussion rate of 120r/min, and the time for shaking absorption is 3h.
（3）It will be positioned under electron beam irradiation light by the processed marine product of chitosan microball and carry out electron beam irradiation,
The dosage of the electron beam irradiation light is 10kGy.
Wherein, the chitosan microball is prepared using following methods：
Gradually 140 parts by weight acetic acid solutions are added in 4 parts by weight chitosans first, 30min is stirred with the rate of 200r/min,
So that chitosan is dissolved in acetic acid solution, then deepfreeze 18 hours then will then by chitosan inhalation syringe
Chitosan in syringe is added dropwise in condensation water, band chitosan be condensed into after sphere washed repeatedly with deionized water as
Then neutrality is dried, wherein the condensation water is prepared using following methods：It is added 2 in the sodium hydroxide of 45 parts by weight
The ethyl acetate of parts by weight, uniform stirring.
Wherein, the chitosan is prepared by following methods：
（1）The shrimp shell of 5 parts by weight is put in the hydrochloric acid of the 1mol/L of 600 parts by weight and impregnates 3h, put after then taking out shrimp shell
Enter and continue to impregnate 9h in the hydrochloric acid of 1000 parts by weight of same concentration, in the sodium hydroxide solution of 500 parts by weight 3% after then taking out
In boil 60min, dry bleaching under daylight again after solid is taken out, obtain chitin；
（2）Then chitin is immersed in the sodium hydroxide that mass fraction is 45%, 3h is kept the temperature at 95 DEG C.
The preferred embodiment of the present invention 3 is detected below：
Ciguatoxin solution is prepared first：It takes malicious algae culturing liquid 400ml to centrifuge 10min at 6000r/min in centrifuge, goes
Continue to suspend again except collecting precipitation after the supernatant in centrifuge tube and being added in the acetic acid solution of 0.01mol/L, in ice-water bath
Ultrasonication uses work power for 600W, takes 3s, stops the interval of 3s, West supernatant vacuum freeze drying.
Then the processing by the ciguatoxin solution Jing Guo embodiment 3 obtains ciguatoxin by fluoroscopic examination
Removal rate reached 97%, have good elimination effect.
Claims (10)
1. a kind of method of electron beam irradiation and chitosan microball interaction degradation ciguatoxin, which is characterized in that including following step
Suddenly：
（1）Marine product containing ciguatoxin is subjected to pre-treatment；
（2）Marine product Jing Guo pre-treatment is impregnated jointly with chitosan microball, it is then molten by acetic acid solution and sodium hydroxide
The pH that liquid adjusts soak is 6 ~ 8, and it is 13 ~ 20 DEG C to keep the temperature of soak, is adsorbed；
（3）It will be positioned under electron beam irradiation light by the processed marine product of chitosan microball and carry out electron beam irradiation.
2. the method for a kind of electron beam irradiation according to claim 1 and chitosan microball interaction degradation ciguatoxin,
It is characterized in that, the pre-treatment includes the following steps：Marine product containing ciguatoxin is cleaned with tap water, is dripped
It is dry, it is marinated that sodium carbonate liquor is then added.
3. the method for a kind of electron beam irradiation according to claim 2 and chitosan microball interaction degradation ciguatoxin,
It is characterized in that：The marinated temperature is at 4 ~ 5 DEG C, and the marinated time is 3 ~ 5h, the quality point of the sodium carbonate liquor
Number is 1 ~ 1.5%.
4. the method for a kind of electron beam irradiation according to claim 1 and chitosan microball interaction degradation ciguatoxin,
It is characterized in that, the chitosan microball is prepared using following methods：
Gradually 120 ~ 160 parts by weight acetic acid solutions are added in 3 ~ 4 parts by weight chitosans first, with the rate of 200 ~ 300r/min
Stir 30 ~ 35min so that chitosan is dissolved in acetic acid solution, and then deepfreeze 18 ~ 20 hours, then inhale chitosan
Enter in syringe, then the chitosan in syringe is added dropwise in condensation water, band chitosan is spent after being condensed into sphere
Ionized water is washed repeatedly as neutrality, is then dried.
5. the method for a kind of electron beam irradiation according to claim 4 and chitosan microball interaction degradation ciguatoxin,
It is characterized in that, the condensation water is prepared using following methods：2 ~ 3 parts by weight are added in the sodium hydroxide of 40 ~ 50 parts by weight
Ethyl acetate, uniform stirring.
6. the method for a kind of electron beam irradiation according to claim 4 and chitosan microball interaction degradation ciguatoxin,
It is characterized in that, the chitosan is prepared by following methods：
（1）The shrimp shell of 5 ~ 6 parts by weight is put in 3 ~ 4h of immersion in the hydrochloric acid of the 1mol/LS of 600 ~ 700 parts by weight, then by shrimp shell
It is put into after taking-up in the hydrochloric acid of 1000 ~ 1200 parts by weight of same concentration and continues 8 ~ 9h of immersion, in 500 ~ 550 weight after then taking out
40 ~ 100min is boiled in part 3% sodium hydroxide solution, bleaching is dried under daylight again after solid is taken out, obtains chitin；
（2）Then chitin is immersed in the sodium hydroxide that mass fraction is 40 ~ 50%, 2 ~ 3h is kept the temperature at 90 ~ 95 DEG C.
7. the method for a kind of electron beam irradiation according to claim 1 and chitosan microball interaction degradation ciguatoxin,
It is characterized in that:The step（2）In adsorption process be concussion adsorb.
8. the method for a kind of electron beam irradiation according to claim 7 and chitosan microball interaction degradation ciguatoxin,
It is characterized in that:The concussion adsorption process is to shake whole system with the concussion rate of 100 ~ 120r/min on shaking table
It swings, the time for shaking absorption is 3 ~ 5h.
9. the method for a kind of electron beam irradiation according to claim 1 and chitosan microball interaction degradation ciguatoxin,
It is characterized in that:The dosage of the electron beam irradiation is 5 ~ 10kGy.
10. the side of a kind of electron beam irradiation and chitosan microball interaction degradation ciguatoxin according to claim 1 or 9
Method, it is characterised in that:The electron beam irradiation uses dosage for 10kGy.
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