CN108276504A - A kind of technique for extracting high purity pectin from apple pomace - Google Patents
A kind of technique for extracting high purity pectin from apple pomace Download PDFInfo
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- CN108276504A CN108276504A CN201810068388.7A CN201810068388A CN108276504A CN 108276504 A CN108276504 A CN 108276504A CN 201810068388 A CN201810068388 A CN 201810068388A CN 108276504 A CN108276504 A CN 108276504A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
- C08B37/0048—Processes of extraction from organic materials
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a kind of from apple pomace extracts the technique of high purity pectin, is related to pectin extraction technical field, includes the following steps：(1) rinsing removal of impurities, (2) powder processed, (3) acid carry, (4) decoloration, (5) concentration, (6) pectin precipitation, (7) purifying, (8) drying, powder processed.The present invention removes soluble sugar and partial pigment in apple pomace by rising operation; the Rapid Leaching of pectin when making apple pomace granulating by powder processed operation to promote follow-up acid to carry; contained pigment in pectin extracting soln is effectively removed by decolorization operations; pectin rate of deposition and pectin purity are improved by the use of pectin precipitating reagent; the galacturonic acid content in final made pectin finished product is set to reach 85% or more, pectin extraction rate reaches 90% or more.
Description
Technical field：
The present invention relates to pectin extraction technical fields, and in particular to a kind of work for extracting high purity pectin from apple pomace
Skill.
Background technology：
Pectin is a kind of polysaccharide high-molecular compound, is a kind of constituent of cell wall, is existed with cellulose,
Molecular weight is 1-40 ten thousand.Pectin finished product is a kind of powdered substance, and shallow white or light yellow can be used for preparing jam, jelly,
It can also be used to manufacture fruity cotton candy, table jelly squares, Turkish delight and sugar-free candy etc..The aluminium salt of pectin and pectin can inhibit
Absorption of the enteron aisle to cholesterol and triglyceride, can be used as the auxiliary treatment of the angiocardiopathies such as artery sclerosis.
Apple pomace is made of pericarp, fruit stone and remaining pulp, more containing soluble sugar, vitamin, minerals, cellulose etc.
Kind substance, is good succulent fodder resource.Currently, in addition to a small amount of apple pomace is used as feed, other are largely then worked as
Make waste disposal, greatly waste resource, polluted environment.
In order to promote the economic value of apple pomace, our company carries out the extraction of pectin using apple pomace as raw material.Pass through work
Skill step is continued to optimize, and pectin extraction rate and pectin purity are improved, to which the pectin finished product of high-quality be made.
Invention content：
Technical problem to be solved by the present invention lies in one kind is provided, operation is simple and can significantly improve pectin extraction
The technique that high purity pectin is extracted in the slave apple pomace of rate and pectin purity.
The technical problems to be solved by the invention are realized using technical solution below：
A kind of technique for extracting high purity pectin from apple pomace, includes the following steps：
(1) rinsing removal of impurities：50-60 DEG C of warm water, solid-liquid ratio 1 are added into apple pomace:3-8, with the stirring of 15-30 r/min
Speed takes filter residue in 50-60 DEG C of insulated and stirred 5-15 min, centrifugal filtration；
(2) powder processed：The freeze-dried machine of filter residue is dehydrated to constant weight, and 60-100 mesh powder is made using pulverizer, i.e.,
Obtain apple ground-slag；
(3) acid carries：Apple ground-slag is put into extraction tank, 80-90 DEG C of hot water, solid-liquid ratio 1 are added:5-10, with dilute salt
Acid for adjusting pH value then with the mixing speed of 60-120 r/min in 80-90 DEG C of insulated and stirred 30-300 min, and heats up to 2
To reflux state insulated and stirred 5-15 min, centrifugal filtration takes filtrate；
(4) it decolourizes：Filtrate is transferred in decoloration slot, and is warming up to after 70-80 DEG C and is added apple pomace dried bean noodles amount 0.5-2%'s
Decolorising agent, then with the mixing speed of 15-30 r/min in 70-80 DEG C of insulated and stirred 10-30 min, centrifugal filtration filters out decoloration
Agent takes filtrate；
(5) it concentrates：Filtrate after decoloration is transferred in evaporator, 35-45% is dropped to through being concentrated in vacuo to mass water content,
Up to concentration glue；
(6) pectin precipitates：Concentration glue is heated to 50-60 DEG C, pectin precipitating reagent is added, is sufficiently stirred and in 50-
60 DEG C of heat preservations stand 0.5-1 h, and gained precipitation washs 2-3 times to get crude pectin with 70% ethyl alcohol；
(7) it purifies：Into crude pectin, 95% ethyl alcohol of the weight such as addition rinses 2-3 times to get wet pectin；
(8) drying, powder processed：It is 8-10% that wet pectin is dried to water content at 50-60 DEG C, obtains dry fruit glue, and will
Dry fruit glue is ground to 60-80 mesh to get pectin finished product.
The decolorising agent selects activated carbon.
The one kind of the pectin precipitating reagent in 95% ethyl alcohol, saturation aluminum sulfate solution.
The decolorising agent is by 4A molecular screen primary powders, poly-aspartic-acid, N hydroxymethyl acrylamide and dimethacrylate
Magnesium is made, and preparation method is：Poly-aspartic-acid saturated solution is prepared using 20-25 DEG C of deionized water, and passes through sulfuric acid tune
PH stable is saved in 4-4.5,4A molecular screen primary powders is added, is heated to reflux state insulated and stirred 15-30 min, is then added
N hydroxymethyl acrylamide and magnesium dimethacrylate continue the insulated and stirred 0.5-3 h that flow back, and gained mixture is through standing nature
It is cooled to room temperature, filters, drying to constant weight at 60-70 DEG C for filter residue, and micro mist is made through micronizer in obtained solid, most passes through afterwards
35-60 mesh particles are made to get decolorising agent in granulator.
The quality of the poly-aspartic-acid, 4A molecular screen primary powders, N hydroxymethyl acrylamide and magnesium dimethacrylate
Than for 10-15:25-40:1-5:5-10.
Reaction mechanism：With N hydroxymethyl acrylamide esterification, while N- methylol propylene occur for poly-aspartic-acid
With magnesium dimethacrylate polymerisation occurs for amide, and the high-molecular compound of generation is attached to 4A molecular screen primary powder surface voids
On, to enhance the decolorizing effect of 4A molecular sieves.
Making decolorising agent by oneself has the decolorizing effect of analogy activated carbon, and can reduce the decoloration loss amount of pectin.
The pectin precipitating reagent is made of food-grade polyglutamic acid and polyethylene glycol 400, and preparation method is：It utilizes
20-25 DEG C of deionized water synthetic food grade polyglutamic acid saturated solution, and pH stable is adjusted in 4-4.5 by sulfuric acid, then
Polyethylene glycol 400 is added, is heated to reflux state insulated and stirred 0.5-3 h, gained mixture is sent after cooled to room temperature
Enter in freeze drier, micro mist is made to get pectin precipitating reagent through micronizer in dry obtained solid.
The food-grade polyglutamic acid, polyethylene glycol 400 mass ratio be 10-15:10-15.
Reaction mechanism：With polyethylene glycol 400 esterification occurs for polyglutamic acid.
Self-control pectin precipitating reagent, which has, is significantly better than the pectin sedimentation effect of ethyl alcohol, aluminum sulfate, improve pectin extraction rate and
Pectin purity.
The beneficial effects of the invention are as follows：The present invention removes soluble sugar and part color in apple pomace by rising operation
It is efficient to propose operation by acid for element, the Rapid Leaching of pectin when making apple pomace granulating by powder processed operation to promote follow-up acid to carry
Pectin is extracted, contained pigment in pectin extracting soln is effectively removed by decolorization operations, concentration of pectin is increased by concentration operation
In favor of the precipitation of follow-up pectin, pectin rate of deposition and pectin purity are improved by the use of pectin precipitating reagent, made final made
Galacturonic acid content in pectin finished product reaches 85% or more, and pectin extraction rate reaches 90% or more.
Specific implementation mode：
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
(1) rinsing removal of impurities：50-60 DEG C of warm water, solid-liquid ratio 1 are added into 25 kg apple pomaces:5, with the stirring of 30 r/min
Speed takes filter residue in 50-60 DEG C of 15 min of insulated and stirred, centrifugal filtration；
(2) powder processed：The freeze-dried machine of filter residue is dehydrated to constant weight, and 60 mesh powder are made to get apple using pulverizer
Pomace powder；
(3) acid carries：Apple ground-slag is put into extraction tank, 80-90 DEG C of hot water, solid-liquid ratio 1 are added:5, with dilute hydrochloric acid tune
PH value is saved to 2, then with the mixing speed of 120 r/min in 80-90 DEG C of 3 h of insulated and stirred, and is warming up to reflux state heat preservation
10 min are stirred, centrifugal filtration takes filtrate；
(4) it decolourizes：Filtrate is transferred in decoloration slot, and is warming up to the decoloration that apple pomace dried bean noodles amount 2% is added after 70-80 DEG C
Agent (activated carbon), then with the mixing speed of 30 r/min in 70-80 DEG C of 15 min of insulated and stirred, centrifugal filtration filters out decolorising agent,
Take filtrate；
(5) it concentrates：Filtrate after decoloration is transferred in evaporator, 35-45% is dropped to through being concentrated in vacuo to mass water content,
Up to concentration glue；
(6) pectin precipitates：Concentration glue is heated to 50-60 DEG C, pectin precipitating reagent (95% ethyl alcohol) is added, fully stirs
It mixes and stands 1 h in 50-60 DEG C of heat preservation, gained precipitation washs 2 times to get crude pectin with 70% ethyl alcohol；
(7) it purifies：Into crude pectin, 95% ethyl alcohol of the weight such as addition rinses 2 times to get wet pectin；
(8) drying, powder processed：It is 8-10% that wet pectin is dried to water content at 50-60 DEG C, obtains dry fruit glue, and will
Dry fruit glue is ground to 80 mesh to get pectin finished product.
Embodiment 2
(1) rinsing removal of impurities：50-60 DEG C of warm water, solid-liquid ratio 1 are added into 25 kg apple pomaces:5, with the stirring of 30 r/min
Speed takes filter residue in 50-60 DEG C of 15 min of insulated and stirred, centrifugal filtration；
(2) powder processed：The freeze-dried machine of filter residue is dehydrated to constant weight, and 60 mesh powder are made to get apple using pulverizer
Pomace powder；
(3) acid carries：Apple ground-slag is put into extraction tank, 80-90 DEG C of hot water, solid-liquid ratio 1 are added:5, with dilute hydrochloric acid tune
PH value is saved to 2, then with the mixing speed of 120 r/min in 80-90 DEG C of 3 h of insulated and stirred, and is warming up to reflux state heat preservation
10 min are stirred, centrifugal filtration takes filtrate；
(4) it decolourizes：Filtrate is transferred in decoloration slot, and is warming up to the decoloration that apple pomace dried bean noodles amount 2% is added after 70-80 DEG C
Agent (activated carbon), then with the mixing speed of 30 r/min in 70-80 DEG C of 15 min of insulated and stirred, centrifugal filtration filters out decolorising agent,
Take filtrate；
(5) it concentrates：Filtrate after decoloration is transferred in evaporator, 35-45% is dropped to through being concentrated in vacuo to mass water content,
Up to concentration glue；
(6) pectin precipitates：Concentration glue is heated to 50-60 DEG C, adds pectin precipitating reagent (saturation aluminum sulfate solution),
It is sufficiently stirred and stands 1 h in 50-60 DEG C of heat preservation, gained precipitation washs 2 times to get crude pectin with 70% ethyl alcohol；
(7) it purifies：Into crude pectin, 95% ethyl alcohol of the weight such as addition rinses 2 times to get wet pectin；
(8) drying, powder processed：It is 8-10% that wet pectin is dried to water content at 50-60 DEG C, obtains dry fruit glue, and will
Dry fruit glue is ground to 80 mesh to get pectin finished product.
Embodiment 3
(1) rinsing removal of impurities：50-60 DEG C of warm water, solid-liquid ratio 1 are added into 25 kg apple pomaces:5, with the stirring of 30 r/min
Speed takes filter residue in 50-60 DEG C of 15 min of insulated and stirred, centrifugal filtration；
(2) powder processed：The freeze-dried machine of filter residue is dehydrated to constant weight, and 60 mesh powder are made to get apple using pulverizer
Pomace powder；
(3) acid carries：Apple ground-slag is put into extraction tank, 80-90 DEG C of hot water, solid-liquid ratio 1 are added:5, with dilute hydrochloric acid tune
PH value is saved to 2, then with the mixing speed of 120 r/min in 80-90 DEG C of 3 h of insulated and stirred, and is warming up to reflux state heat preservation
10 min are stirred, centrifugal filtration takes filtrate；
(4) it decolourizes：Filtrate is transferred in decoloration slot, and is warming up to the decoloration that apple pomace dried bean noodles amount 2% is added after 70-80 DEG C
Agent, then with the mixing speed of 30 r/min in 70-80 DEG C of 15 min of insulated and stirred, centrifugal filtration filters out decolorising agent, takes filtrate；
(5) it concentrates：Filtrate after decoloration is transferred in evaporator, 35-45% is dropped to through being concentrated in vacuo to mass water content,
Up to concentration glue；
(6) pectin precipitates：Concentration glue is heated to 50-60 DEG C, pectin precipitating reagent (95% ethyl alcohol) is added, fully stirs
It mixes and stands 1 h in 50-60 DEG C of heat preservation, gained precipitation washs 2 times to get crude pectin with 70% ethyl alcohol；
(7) it purifies：Into crude pectin, 95% ethyl alcohol of the weight such as addition rinses 2 times to get wet pectin；
(8) drying, powder processed：It is 8-10% that wet pectin is dried to water content at 50-60 DEG C, obtains dry fruit glue, and will
Dry fruit glue is ground to 80 mesh to get pectin finished product.
The preparation of decolorising agent：Poly-aspartic-acid saturated solution (the poly- lucid asparagus of 15 g is prepared using 20-25 DEG C of deionized water
Propylhomoserin), and pH stable is adjusted in 4-4.5 by sulfuric acid, 40 g 4A molecular screen primary powders are added, reflux state guarantor is heated to
Temperature 30 min of stirring, are then added 5 g N hydroxymethyl acrylamides and 8 g magnesium dimethacrylates, continue the insulated and stirred that flows back
2 h, gained mixture is through standing cooled to room temperature, and filtering, drying to constant weight at 60-70 DEG C for filter residue, obtained solid warp
Micro mist is made in micronizer, and 60 mesh particles most are made to get decolorising agent through granulator afterwards.
Embodiment 4
(1) rinsing removal of impurities：50-60 DEG C of warm water, solid-liquid ratio 1 are added into 25 kg apple pomaces:5, with the stirring of 30 r/min
Speed takes filter residue in 50-60 DEG C of 15 min of insulated and stirred, centrifugal filtration；
(2) powder processed：The freeze-dried machine of filter residue is dehydrated to constant weight, and 60 mesh powder are made to get apple using pulverizer
Pomace powder；
(3) acid carries：Apple ground-slag is put into extraction tank, 80-90 DEG C of hot water, solid-liquid ratio 1 are added:5, with dilute hydrochloric acid tune
PH value is saved to 2, then with the mixing speed of 120 r/min in 80-90 DEG C of 3 h of insulated and stirred, and is warming up to reflux state heat preservation
10 min are stirred, centrifugal filtration takes filtrate；
(4) it decolourizes：Filtrate is transferred in decoloration slot, and is warming up to the decoloration that apple pomace dried bean noodles amount 2% is added after 70-80 DEG C
Agent (activated carbon), then with the mixing speed of 30 r/min in 70-80 DEG C of 15 min of insulated and stirred, centrifugal filtration filters out decolorising agent,
Take filtrate；
(5) it concentrates：Filtrate after decoloration is transferred in evaporator, 35-45% is dropped to through being concentrated in vacuo to mass water content,
Up to concentration glue；
(6) pectin precipitates：Concentration glue is heated to 50-60 DEG C, pectin precipitating reagent is added, is sufficiently stirred and in 50-
60 DEG C of heat preservations stand 1 h, and gained precipitation washs 2 times to get crude pectin with 70% ethyl alcohol；
(7) it purifies：Into crude pectin, 95% ethyl alcohol of the weight such as addition rinses 2 times to get wet pectin；
(8) drying, powder processed：It is 8-10% that wet pectin is dried to water content at 50-60 DEG C, obtains dry fruit glue, and will
Dry fruit glue is ground to 80 mesh to get pectin finished product.
The preparation of pectin precipitating reagent：Utilize 20-25 DEG C of deionized water synthetic food grade polyglutamic acid saturated solution (10 g
Food-grade polyglutamic acid), and pH stable is adjusted in 4-4.5 by sulfuric acid, 15 g polyethylene glycol 400s are added, are heated to
2 h of reflux state insulated and stirred, gained mixture are sent into after cooled to room temperature in freeze drier, dry obtained solid
Micro mist is made to get pectin precipitating reagent through micronizer.
Embodiment 5
(1) rinsing removal of impurities：50-60 DEG C of warm water, solid-liquid ratio 1 are added into 25 kg apple pomaces:5, with the stirring of 30 r/min
Speed takes filter residue in 50-60 DEG C of 15 min of insulated and stirred, centrifugal filtration；
(2) powder processed：The freeze-dried machine of filter residue is dehydrated to constant weight, and 60 mesh powder are made to get apple using pulverizer
Pomace powder；
(3) acid carries：Apple ground-slag is put into extraction tank, 80-90 DEG C of hot water, solid-liquid ratio 1 are added:5, with dilute hydrochloric acid tune
PH value is saved to 2, then with the mixing speed of 120 r/min in 80-90 DEG C of 3 h of insulated and stirred, and is warming up to reflux state heat preservation
10 min are stirred, centrifugal filtration takes filtrate；
(4) it decolourizes：Filtrate is transferred in decoloration slot, and is warming up to the decoloration that apple pomace dried bean noodles amount 2% is added after 70-80 DEG C
Agent, then with the mixing speed of 30 r/min in 70-80 DEG C of 15 min of insulated and stirred, centrifugal filtration filters out decolorising agent, takes filtrate；
(5) it concentrates：Filtrate after decoloration is transferred in evaporator, 35-45% is dropped to through being concentrated in vacuo to mass water content,
Up to concentration glue；
(6) pectin precipitates：Concentration glue is heated to 50-60 DEG C, pectin precipitating reagent is added, is sufficiently stirred and in 50-
60 DEG C of heat preservations stand 1h, and gained precipitation washs 2 times to get crude pectin with 70% ethyl alcohol；
(7) it purifies：Into crude pectin, 95% ethyl alcohol of the weight such as addition rinses 2 times to get wet pectin；
(8) drying, powder processed：It is 8-10% that wet pectin is dried to water content at 50-60 DEG C, obtains dry fruit glue, and will
Dry fruit glue is ground to 80 mesh to get pectin finished product.
The preparation of decolorising agent：Poly-aspartic-acid saturated solution (the poly- lucid asparagus of 15g is prepared using 20-25 DEG C of deionized water
Propylhomoserin), and pH stable is adjusted in 4-4.5 by sulfuric acid, 40g 4A molecular screen primary powders are added, reflux state heat preservation is heated to
30min is stirred, 5g N hydroxymethyl acrylamides and 8g magnesium dimethacrylates is then added, continues the insulated and stirred 2h that flows back, institute
Mixture is obtained through standing cooled to room temperature, filtering, drying to constant weight at 60-70 DEG C for filter residue, and obtained solid is through Ultramicro-powder
Micro mist is made in broken machine, and 60 mesh particles most are made to get decolorising agent through granulator afterwards.
The preparation of pectin precipitating reagent：Utilize 20-25 DEG C of deionized water synthetic food grade polyglutamic acid saturated solution (10g
Food-grade polyglutamic acid), and pH stable is adjusted in 4-4.5 by sulfuric acid, 15g polyethylene glycol 400s are added, are heated to back
Stream mode insulated and stirred 2h, gained mixture are sent into after cooled to room temperature in freeze drier, dry obtained solid warp
Micro mist is made to get pectin precipitating reagent in micronizer.
Reference examples 1
(1) rinsing removal of impurities：50-60 DEG C of warm water, solid-liquid ratio 1 are added into 25kg apple pomaces:5, with the stirring speed of 30r/min
It spends in 50-60 DEG C of insulated and stirred 15min, centrifugal filtration takes filter residue；
(2) acid carries：Filter residue is put into extraction tank, 80-90 DEG C of hot water, solid-liquid ratio 1 are added:5, adjust pH with dilute hydrochloric acid
Value then with the mixing speed of 120r/min in 80-90 DEG C of insulated and stirred 3h, and is warming up to reflux state insulated and stirred to 2
10min, centrifugal filtration take filtrate；
(3) it decolourizes：Filtrate is transferred in decoloration slot, and is warming up to the decoloration that apple pomace dried bean noodles amount 2% is added after 70-80 DEG C
Agent (activated carbon), then with the mixing speed of 30r/min in 70-80 DEG C of insulated and stirred 15min, centrifugal filtration filters out decolorising agent, takes
Filtrate；
(4) it concentrates：Filtrate after decoloration is transferred in evaporator, 35-45% is dropped to through being concentrated in vacuo to mass water content,
Up to concentration glue；
(5) pectin precipitates：Concentration glue is heated to 50-60 DEG C, pectin precipitating reagent (95% ethyl alcohol) is added, fully stirs
It mixes and stands 1h in 50-60 DEG C of heat preservation, gained precipitation washs 2 times to get crude pectin with 70% ethyl alcohol；
(6) it purifies：Into crude pectin, 95% ethyl alcohol of the weight such as addition rinses 2 times to get wet pectin；
(7) drying, powder processed：It is 8-10% that wet pectin is dried to water content at 50-60 DEG C, obtains dry fruit glue, and will
Dry fruit glue is ground to 80 mesh to get pectin finished product.
Reference examples 2
(1) powder processed：25kg apple residue freeze driers are dehydrated to constant weight, and 60 mesh powder are made using pulverizer,
Up to apple ground-slag；
(2) acid carries：Apple ground-slag is put into extraction tank, 80-90 DEG C of hot water, solid-liquid ratio 1 are added:5, with dilute hydrochloric acid tune
PH value is saved to 2, then with the mixing speed of 120r/min in 80-90 DEG C of insulated and stirred 3h, and reflux state heat preservation is warming up to and stirs
10min is mixed, centrifugal filtration takes filtrate；
(3) it decolourizes：Filtrate is transferred in decoloration slot, and is warming up to the decoloration that apple pomace dried bean noodles amount 2% is added after 70-80 DEG C
Agent (activated carbon), then with the mixing speed of 30r/min in 70-80 DEG C of insulated and stirred 15min, centrifugal filtration filters out decolorising agent, takes
Filtrate；
(4) it concentrates：Filtrate after decoloration is transferred in evaporator, 35-45% is dropped to through being concentrated in vacuo to mass water content,
Up to concentration glue；
(5) pectin precipitates：Concentration glue is heated to 50-60 DEG C, pectin precipitating reagent (95% ethyl alcohol) is added, fully stirs
It mixes and stands 1h in 50-60 DEG C of heat preservation, gained precipitation washs 2 times to get crude pectin with 70% ethyl alcohol；
(6) it purifies：Into crude pectin, 95% ethyl alcohol of the weight such as addition rinses 2 times to get wet pectin；
(7) drying, powder processed：It is 8-10% that wet pectin is dried to water content at 50-60 DEG C, obtains dry fruit glue, and will
Dry fruit glue is ground to 80 mesh to get pectin finished product.
Embodiment 6
Embodiment 1-5, reference examples 1-2 are utilized respectively to carrying out pectin extraction with batch apple pomace, and is measured in pectin finished product
Galacturonic acid content and pectin extraction rate, as shown in table 1.And the reference examples using equivalent 4A molecular sieves as decolorising agent are set
3。
It should be noted that：Pectin extraction rate refers to the ratio of pectin actual production and contained pectin quality in apple pomace,
The dosage of pectin precipitating reagent is subject to be settled out pectin while being wasted there is no excessive to greatest extent.
1 pectin from apple pomace extraction effect of the present invention of table
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (5)
1. a kind of technique for extracting high purity pectin from apple pomace, which is characterized in that include the following steps：
(1) rinsing removal of impurities：50-60 DEG C of warm water, solid-liquid ratio 1 are added into apple pomace:3-8, with the mixing speed of 15-30r/min
In 50-60 DEG C of insulated and stirred 5-15min, centrifugal filtration takes filter residue；
(2) powder processed：The freeze-dried machine of filter residue is dehydrated to constant weight, and 60-100 mesh powder is made to get apple using pulverizer
Pomace powder；
(3) acid carries：Apple ground-slag is put into extraction tank, 80-90 DEG C of hot water, solid-liquid ratio 1 are added:5-10, with dilute hydrochloric acid tune
PH value is saved to 2, then with the mixing speed of 60-120r/min in 80-90 DEG C of insulated and stirred 30-300min, and is warming up to reflux
State insulated and stirred 5-15min, centrifugal filtration take filtrate；
(4) it decolourizes：Filtrate is transferred in decoloration slot, and is warming up to the decoloration that apple pomace dried bean noodles amount 0.5-2% is added after 70-80 DEG C
Agent, then with the mixing speed of 15-30r/min in 70-80 DEG C of insulated and stirred 10-30min, centrifugal filtration filters out decolorising agent, takes filter
Liquid；
(5) it concentrates：Filtrate after decoloration is transferred in evaporator, through be concentrated in vacuo to mass water content drop to 35-45% to get
Concentrate glue；
(6) pectin precipitates：Concentration glue is heated to 50-60 DEG C, pectin precipitating reagent is added, is sufficiently stirred and in 50-60 DEG C
Heat preservation stands 0.5-1h, and gained precipitation washs 2-3 times to get crude pectin with 70% ethyl alcohol；
(7) it purifies：Into crude pectin, 95% ethyl alcohol of the weight such as addition rinses 2-3 times to get wet pectin；
(8) drying, powder processed：It is 8-10% that wet pectin is dried at 50-60 DEG C to water content, obtains dry fruit glue, and by dry fruit
Glue is ground to 60-80 mesh to get pectin finished product.
2. the technique according to claim 1 for extracting high purity pectin from apple pomace, it is characterised in that：The decolorising agent
Select activated carbon.
3. the technique according to claim 1 for extracting high purity pectin from apple pomace, it is characterised in that：The pectin is heavy
The one kind of shallow lake agent in 95% ethyl alcohol, saturation aluminum sulfate solution.
4. the technique according to claim 1 for extracting high purity pectin from apple pomace, it is characterised in that：The decolorising agent
It is made of 4A molecular screen primary powders, poly-aspartic-acid, N hydroxymethyl acrylamide and magnesium dimethacrylate, preparation method
For：Poly-aspartic-acid saturated solution is prepared using 20-25 DEG C of deionized water, and pH stable is adjusted in 4-4.5 by sulfuric acid,
Add 4A molecular screen primary powders, be heated to reflux state insulated and stirred 15-30min, then be added N hydroxymethyl acrylamide and
Magnesium dimethacrylate, continues the insulated and stirred 0.5-3h that flows back, and gained mixture filters, filter through standing cooled to room temperature
Drying to constant weight at 60-70 DEG C for slag, and micro mist is made through micronizer in obtained solid, and 35-60 mesh most is made through granulator afterwards
Particle is to get decolorising agent.
5. extracting the technique of high purity pectin in the slave apple pomace stated according to claim 4, it is characterised in that：The poly- lucid asparagus
Propylhomoserin, 4A molecular screen primary powders, N hydroxymethyl acrylamide and magnesium dimethacrylate mass ratio be 10-15:25-40:1-5:
5-10。
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| CN108715620A (en) * | 2018-08-08 | 2018-10-30 | 安徽省美极调味食品有限公司 | The extraction process of high-purity biology pectin |
| CN109111532A (en) * | 2018-09-30 | 2019-01-01 | 三门峡源丰生物科技有限公司 | A kind of pectin extraction method |
| CN110156912A (en) * | 2019-06-19 | 2019-08-23 | 河南佰卓果胶生物科技有限公司 | A kind of process for producing pectin |
| CN113621090A (en) * | 2021-07-08 | 2021-11-09 | 浙江工业大学 | Pectin multi-stage decoloring method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108164530A (en) * | 2018-01-24 | 2018-06-15 | 安徽省百花香料香精有限公司 | A kind of environmentally friendly process for purification of theobromine |
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| CN109111532A (en) * | 2018-09-30 | 2019-01-01 | 三门峡源丰生物科技有限公司 | A kind of pectin extraction method |
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| CN110156912A (en) * | 2019-06-19 | 2019-08-23 | 河南佰卓果胶生物科技有限公司 | A kind of process for producing pectin |
| CN113621090A (en) * | 2021-07-08 | 2021-11-09 | 浙江工业大学 | Pectin multi-stage decoloring method |
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