CN108273496B - Preparation method and application of biomimetic enzyme based on bacterial cellulose - Google Patents
Preparation method and application of biomimetic enzyme based on bacterial cellulose Download PDFInfo
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- CN108273496B CN108273496B CN201810218925.1A CN201810218925A CN108273496B CN 108273496 B CN108273496 B CN 108273496B CN 201810218925 A CN201810218925 A CN 201810218925A CN 108273496 B CN108273496 B CN 108273496B
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- 229920002749 Bacterial cellulose Polymers 0.000 title claims abstract description 45
- 239000005016 bacterial cellulose Substances 0.000 title claims abstract description 45
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 14
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 230000003592 biomimetic effect Effects 0.000 title claims abstract description 11
- AMWRITDGCCNYAT-UHFFFAOYSA-L hydroxy(oxo)manganese;manganese Chemical compound [Mn].O[Mn]=O.O[Mn]=O AMWRITDGCCNYAT-UHFFFAOYSA-L 0.000 claims abstract description 60
- 229920000049 Carbon (fiber) Polymers 0.000 claims abstract description 36
- 239000004917 carbon fiber Substances 0.000 claims abstract description 36
- 239000012286 potassium permanganate Substances 0.000 claims abstract description 35
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000002131 composite material Substances 0.000 claims abstract description 32
- 239000002086 nanomaterial Substances 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000004108 freeze drying Methods 0.000 claims abstract description 9
- 239000004964 aerogel Substances 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 7
- 238000007323 disproportionation reaction Methods 0.000 claims abstract description 6
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 claims abstract description 5
- 238000003837 high-temperature calcination Methods 0.000 claims abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- 238000002791 soaking Methods 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 13
- 239000008367 deionised water Substances 0.000 claims description 12
- 229910021641 deionized water Inorganic materials 0.000 claims description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- 239000000017 hydrogel Substances 0.000 claims description 8
- 239000000835 fiber Substances 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 238000003760 magnetic stirring Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 238000001354 calcination Methods 0.000 claims description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 239000003960 organic solvent Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000011068 loading method Methods 0.000 claims description 2
- -1 superoxide anions Chemical class 0.000 abstract description 10
- 239000011664 nicotinic acid Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 238000010000 carbonizing Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
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- 239000002121 nanofiber Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000001000 micrograph Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 238000006555 catalytic reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229910021397 glassy carbon Inorganic materials 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229910004613 CdTe Inorganic materials 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920000557 Nafion® Polymers 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 238000003917 TEM image Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000002134 carbon nanofiber Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 238000001523 electrospinning Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008104 plant cellulose Substances 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 238000001132 ultrasonic dispersion Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J23/00—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00
- B01J23/16—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00 of arsenic, antimony, bismuth, vanadium, niobium, tantalum, polonium, chromium, molybdenum, tungsten, manganese, technetium or rhenium
- B01J23/32—Manganese, technetium or rhenium
- B01J23/34—Manganese
-
- B01J35/23—
-
- B01J35/393—
-
- B01J35/58—
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J37/00—Processes, in general, for preparing catalysts; Processes, in general, for activation of catalysts
- B01J37/02—Impregnation, coating or precipitation
- B01J37/03—Precipitation; Co-precipitation
- B01J37/036—Precipitation; Co-precipitation to form a gel or a cogel
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J37/00—Processes, in general, for preparing catalysts; Processes, in general, for activation of catalysts
- B01J37/08—Heat treatment
- B01J37/082—Decomposition and pyrolysis
- B01J37/084—Decomposition of carbon-containing compounds into carbon
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J37/00—Processes, in general, for preparing catalysts; Processes, in general, for activation of catalysts
- B01J37/08—Heat treatment
- B01J37/082—Decomposition and pyrolysis
- B01J37/088—Decomposition of a metal salt
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J37/00—Processes, in general, for preparing catalysts; Processes, in general, for activation of catalysts
- B01J37/32—Freeze drying, i.e. lyophilisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
Abstract
The invention discloses a preparation method and application of a biomimetic enzyme based on bacterial cellulose. The method is characterized in that bacterial cellulose is carbonized at high temperature to synthesize carbon fibers, and a high-concentration potassium permanganate solution is loaded in the bacterial cellulose through the exchange function of water by utilizing the porous structure and the high water holding capacity of the bacterial cellulose. And then freeze-drying to form the bacterial cellulose/potassium permanganate aerogel. And then carbonizing the bacterial cellulose by high-temperature calcination to form carbon fibers, and decomposing the potassium permanganate at high temperature to generate manganese oxide nanoparticles. The carbon fiber/manganese oxide composite nano material prepared by the method has good bionic enzyme activity, can catalyze the disproportionation reaction of superoxide anions, is applied to the preparation of superoxide anion sensors, and has high application value in the fields of biology and medicine. The method has low cost and simple operation, and is convenient for mass production, popularization and application.
Description
Technical Field
The invention relates to a preparation method of a biomimetic enzyme of bacterial cellulose, which utilizes natural bacterial cellulose loaded potassium permanganate to form a carbon fiber/manganese oxide composite nanofiber material through high-temperature calcination, and belongs to the technology of polymer-based functional composite materials.
Background
The carbon fiber is a novel carbon-based nano material, and has excellent mechanical strength, large volume-mass ratio and porous network structure. The carbon fiber has the diameter of nanometer size and the general performance of nanometer material, and the boundary site on the surface of the carbon fiber can promote the surface electron transfer for electrochemical apparatus or electrochemical analysis. Many methods for preparing carbon fibers, the most common of which is electrospinning, can synthesize nanofibers of several nanometers to several micrometers in diameter using polymer solutions or melts. In recent years, experts have developed simpler methods for preparing carbon nanofibers. Some natural plant fibers, such as cotton, leaves, bacterial cellulose and other materials, are chemically or physically processed to synthesize the carbon fibers for the energy and sensing fields.
Bacterial cellulose is a cellulose derived from a particular bacterium such asAcetobacter、AgrobacteriumAnd the like. Compared with common plant cellulose, the bacterial cellulose has the advantages of high purity, small diameter,High mechanical strength, good water holding capacity and the like, and is an ideal carrier for synthesizing other nano materials. For example, a silver nanoparticle-doped composite nanofiber may be synthesized using bacterial cellulose as a template; the synthetic bacterial fiber/CdTe quantum dot composite material is used for preparing a pH and glucose sensor; or detecting metal ions such as Cd (II), Pb (II) and the like for synthesizing the carbon fiber by a high-temperature calcination method. However, the research on the synthesis of the carbon fiber-based composite material by utilizing the bacterial cellulose is less, and the related research on the carbon fiber/manganese oxide composite nano material is not reported.
The patent provides a preparation method for preparing a carbon fiber/manganese oxide composite nano material by taking bacterial cellulose and potassium permanganate as raw materials and application of the carbon fiber/manganese oxide composite nano material in superoxide anion detection.
Disclosure of Invention
The invention aims to provide a preparation method of a biomimetic enzyme based on bacterial cellulose, which can be used for simply and effectively preparing a carbon fiber/manganese oxide composite nanofiber material. The composite nano material has the catalytic activity of bionic enzyme, has a strong catalytic action on the disproportionation reaction of superoxide anion, and can be used for biomedical research and the preparation of biosensors.
The preparation method of the biomimetic enzyme based on the bacterial cellulose comprises the following preparation steps:
(1) providing a natural bacterial cellulose;
(2) under magnetic stirring, soaking natural bacterial cellulose in 10% ethanol water solution, soaking in deionized water, and replacing the deionized water to remove organic solvent and other impurities;
(3) soaking the bacterial cellulose soaking material obtained in the step (2) in a potassium permanganate solution, reacting for 1 day under magnetic stirring, loading potassium permanganate, washing and washing the reacted bacterial cellulose with deionized water for 3-5 times, and removing excessive potassium permanganate to obtain a bacterial cellulose/potassium permanganate hydrogel;
(4) freezing the bacterial cellulose/potassium permanganate hydrogel obtained in the step (3) in liquid nitrogen, and then putting the frozen bacterial cellulose/potassium permanganate hydrogel into a freeze drying oven (-80 ℃, 0.021 Mpa) for freeze drying to obtain bacterial fiber/potassium permanganate aerogel;
(5) and (5) calcining the bacterial cellulose/potassium permanganate aerogel obtained in the step (4) at high temperature in a tubular furnace under the protection of nitrogen to form a carbon fiber/manganese oxide composite nano material, thus obtaining the bacterial cellulose-based biomimetic enzyme.
Further, the soaking time of the 10% ethanol aqueous solution in the step (2) is 1 hour, the soaking time in deionized water is 3 days, and the number of times of replacing the deionized water is 3-6 times.
Further, the concentration of the potassium permanganate solution in the step (3) is 1-40 mM, and the soaking time in the potassium permanganate solution is 12-24 hours.
Preferably, the concentration of the potassium permanganate solution in the step (3) is 20 mM.
Further, the time of freezing in liquid nitrogen in the step (4) is 30 minutes, and the freeze-drying time is 12-24 hours.
Preferably, the high-temperature calcination in the step (5) is carried out at a temperature rise rate of 2 ℃ per minute, and is kept at 250 ℃ for 1 hour, then kept at 450 ℃ for 1 hour, and finally kept at 800 ℃ for 2 hours.
The carbon fiber/manganese oxide composite nano material obtained by the preparation method is applied to the biological and medical fields of the activity of bionic enzyme and the catalysis of the disproportionation reaction of superoxide anions.
The invention has the beneficial effects that:
(1) the carbon fiber/manganese oxide composite nano material provided by the invention is formed by high-temperature treatment of bacterial cellulose loaded by potassium permanganate, and is low in synthesis cost and beneficial to popularization and application.
(2) The diameter of the carbon fiber in the carbon fiber/manganese oxide composite nano material obtained by the invention is about 10-30 nm.
(3) When the concentration of potassium permanganate is 20mM, the mass fraction of manganese oxide in the obtained composite material is 1.6-3.5%, and the particle size of manganese oxide is 30-70 nm.
(4) The carbon fiber/manganese oxide composite nano material obtained by the invention has stronger catalytic performance on the disproportionation reaction of superoxide anions and has great application value on biomedical research and preparation of biosensors.
(5) The preparation method provided by the invention is simple and feasible, is easy to operate, widens the application fields of the bacterial cellulose and the nano manganese oxide, endows the bacterial cellulose with a new application field, and has high economic value.
Drawings
In order to make the object and the technical scheme beneficial effects of the invention more clear, the invention provides the following drawings:
fig. 1 is a synthesis method of the carbon fiber/manganese oxide composite nanomaterial of example 1.
FIG. 2 is a scanning electron microscope micrograph of the carbon fiber/manganese oxide composite nanomaterial of example 1.
FIG. 3 is a TEM micrograph of the carbon fiber/manganese oxide composite nanomaterial of example 1.
Fig. 4 is a powder diffraction pattern of the carbon fiber/manganese oxide composite nanomaterial of example 1.
Fig. 5 is a graph showing the electrochemical response of the carbon fiber/manganese oxide composite nanomaterial of example 1 to superoxide anions.
FIG. 6 is a standard curve of the carbon fiber/manganese oxide composite nanomaterial of example 1 for detecting superoxide anions.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
Example 1
The schematic diagram of the synthesis steps is shown in figure 1, and specifically comprises the following steps:
(1) cutting the bacterial cellulose raw material into small blocks of 3cm multiplied by 0.5cm, firstly soaking in 10% ethanol water solution for 1 hour, and then soaking in deionized water for 3 days to remove organic solvent and other impurities.
(2) The bacterial cellulose is soaked in 20mM potassium permanganate solution for 1 day under the action of magnetic stirring. And then taking out and washing the fiber by deionized water for 3 to 5 times to remove excessive potassium permanganate to obtain the bacterial fiber/potassium permanganate hydrogel.
(3) And (3) freezing the bacterial cellulose/potassium permanganate hydrogel in liquid nitrogen for 30 minutes, and then placing the frozen bacterial cellulose/potassium permanganate hydrogel in a freeze drying oven (the temperature is minus 80 ℃ and 0.021 Mpa) for freeze drying for 24 hours to prepare the bacterial fiber/potassium permanganate aerogel.
(4) And (3) calcining the bacterial cellulose/potassium permanganate aerogel in a tubular furnace (under the protection of nitrogen) at 800 ℃ for 2 hours. And naturally cooling to room temperature to obtain the carbon fiber/manganese oxide composite nano material.
The microscopic morphology and characteristic peaks of the carbon fiber/manganese oxide composite nanomaterial can be seen through the scanning electron microscope image of fig. 2, the transmission electron microscope image of fig. 3 and the powder diffraction pattern of fig. 4, which indicates that the carbon fiber/manganese oxide composite nanomaterial is successfully prepared.
Then 1mg of the synthesized carbon fiber/manganese oxide composite nano material and 50 mu L of nafion are added into 450 mu L of ethanol for ultrasonic dispersion, 5 mu L of mixed solution is dripped on the surface of the ground glassy carbon electrode, and the glassy carbon electrode is dried for 2 hours at room temperature. Experiments were then performed with electrochemical responses and detection of superoxide anions at a constant potential of 0.64V. Fig. 5 is an electrochemical response diagram of the carbon fiber/manganese oxide composite nanomaterial to superoxide anions, fig. 6 is a standard detection curve of the carbon fiber/manganese oxide composite nanomaterial to superoxide anions, and fig. 5 and 6 can prove that the carbon fiber/manganese oxide composite nanomaterial can well detect superoxide anion released by tumor cells in a biological system, so that the carbon fiber/manganese oxide composite nanomaterial has biological and medical applications in biomimetic enzymes and catalysis of disproportionation of superoxide anions.
Claims (6)
1. Biological and medical application of biomimetic enzyme based on bacterial cellulose in catalyzing disproportionation reaction of superoxide anion is characterized in that the preparation method of the biomimetic enzyme based on bacterial cellulose comprises the following steps:
(1) providing a natural bacterial cellulose;
(2) under magnetic stirring, soaking natural bacterial cellulose in 10% ethanol water solution, soaking in deionized water, and replacing the deionized water to remove organic solvent and other impurities;
(3) soaking the bacterial cellulose soaking material obtained in the step (2) in a potassium permanganate solution, reacting for 1 day under magnetic stirring, loading potassium permanganate, washing and washing the reacted bacterial cellulose with deionized water for 3-5 times, and removing excessive potassium permanganate to obtain a bacterial cellulose/potassium permanganate hydrogel;
(4) freezing the bacterial cellulose/potassium permanganate hydrogel obtained in the step (3) in liquid nitrogen, and then putting the liquid nitrogen into a freeze drying box at the temperature of minus 80 ℃ and 0.021 Mpa for freeze drying to prepare bacterial fiber/potassium permanganate aerogel;
(5) and (5) calcining the bacterial cellulose/potassium permanganate aerogel obtained in the step (4) at high temperature in a tubular furnace under the protection of nitrogen to form a carbon fiber/manganese oxide composite nano material, thus obtaining the bacterial cellulose-based biomimetic enzyme.
2. The use according to claim 1, wherein the soaking time in 10% ethanol aqueous solution in the step (2) is 1 hour, the soaking time in deionized water is 3 days, and the number of times of replacing deionized water is 3-6 times.
3. The use according to claim 1, wherein the concentration of the potassium permanganate solution in the step (3) is 1-40 mM, and the soaking time in the potassium permanganate solution is 12-24 hours.
4. The use according to claim 3, wherein the potassium permanganate solution of step (3) has a concentration of 20 mM.
5. The use according to claim 1, wherein the freezing time in liquid nitrogen in step (4) is 30 minutes, and the freeze-drying time is 12-24 hours.
6. The application of the method according to claim 1, wherein the high-temperature calcination in the step (5) is carried out at a temperature rise rate of 2 ℃ per minute, and is kept at 250 ℃ for 1 hour, then kept at 450 ℃ for 1 hour, and finally kept at 800 ℃ for 2 hours.
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| CN110143584A (en) * | 2019-05-31 | 2019-08-20 | 西南大学 | A kind of superoxide dismutase biomimetic material and its preparation method and application |
| CN111569881B (en) * | 2020-05-29 | 2021-11-02 | 西南大学 | Preparation method of monatomic biomimetic enzyme, and product and application thereof |
| CN111650261B (en) * | 2020-06-09 | 2022-09-20 | 苏州科技大学 | Conductive ink for electrochemical biosensor and preparation method and application thereof |
| CN112834589B (en) * | 2020-12-31 | 2023-12-22 | 陕西师范大学 | AuQD@CNFs composite material and preparation method and application thereof |
| CN113694964B (en) * | 2021-08-27 | 2022-08-02 | 中国科学院化学研究所 | Bionic laccase system based on polysaccharide/dopamine composite membrane as well as preparation method and application of bionic laccase system |
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