CN108267591A - A kind of immune microsphere chromatographs quick HIT antibody tests test paper - Google Patents
A kind of immune microsphere chromatographs quick HIT antibody tests test paper Download PDFInfo
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- CN108267591A CN108267591A CN201710000252.8A CN201710000252A CN108267591A CN 108267591 A CN108267591 A CN 108267591A CN 201710000252 A CN201710000252 A CN 201710000252A CN 108267591 A CN108267591 A CN 108267591A
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The present invention relates to the technical fields of clinical heparin-induced thrombocytopenia (HIT) detection, and in particular to a kind of immune microsphere chromatography heparin/platelet factor 4 antibody test test paper.Include the following steps：Prepare antibody complex；Prepared by the preparation of test strips carrier film, albumin A latex particle, preparation, sample pad label, seperation film assembling, the assembling of test strips, the clinical sample detection of reacting pad, and dry test strips are placed in aluminium foil bag.The sidestream immune detection method of HIT antibody of the present invention has higher cost benefit, and quantifies level of the detect and assess HIT antibody in the body fluid of patient.Titre of this method for HIT antibody in POCT detecting systems and screening or detection patient body fluid changes to determine the detection method to HIT sensitivities, and HIT antibody tests technology can be made to become routine clinical detection project.
Description
Technical field
The present invention relates to heparin-induced thrombocytopenia inspection technology fields more particularly to a kind of immune microsphere to chromatograph
Quick HIT antibody tests examination method for preparing test paper.
Background technology
HIT is a kind of antibody-mediated syndrome, there is higher incidence and lethality, and the past does not have due to many doctors
Enough understanding and to be difficult to make a definite diagnosis be considered as often a kind of orphan disease.And also without better treatment means other than heparin.
For the incidence height of HIT mainly with heparin usage time, heparin type uses approach, patient class, the factors such as gender and ethnic group
It is related.
Pathogenesis in relation to HIT is not also very clearly, to be widely considered to be at present in blood circulation and heparin sample compound occur
When, PF4 (platelet factor 4) is i.e. in combination with high-affinity, and forming heparin-PF4 (H-PF4) compound, (heparin loses work
Property).After H-PF4 compounds are formed, conformation changes (loosely, to be exposed multiple anti-between the 3rd, 4 cysteine residues
Former epitope, body is interior to occur immune response, generates Immunoglobulin IgG, IgA, IgM) --- IgG-H-PF4 compounds.IgG-
H-PF4 compounds are attached on platelet membrane receptor --- and a large amount of platelet activations and aggregation and platelet counts decline.Blood is small
Plate is extensively after activation, and the release of vesica endoparticle --- activation blood coagulation system, fibrin ferment, which is formed, to be increased.The blood platelet of activation is with coagulating
Blood factor interacts --- thrombosis.
Compared with the thrombopenia that the induction of other reasons generates, HIT Platelets count normal HIT>20*10^9.
Duration of seizure is 5~14 days after heparin is used.
The major complications of HIT are not the hemorrhagic diseases caused by decrease of platelet, but because of platelet antibody reason
Body is in hypercoagulative state, and pathologic thrombus is formed.And arterial thromboembolism incidence is high.In addition, HIT has 50% risk
It can lead to a series of complication, thrombus mostly occurs in vein.Such as the formation of double lower limb deep vein thrombosis, pulmonary embolism, acra
Vein gangrene fingers, toes, penis, or nipples, heart infarction, palsy, mesenteric artery thrombus, limb ischemia and
Amputation (Circulation 1999；100:587-93).
In addition, some patientss local heparin injection site may occur in which pain erythema or cutaneous necrosis.Acute platelet activation
(shiver with cold, myalgia, fever, tachycardia, profuse sweating and nausea etc., rare symptom have acute transient forgetting.) HIT related mortalities
About 18%.But correctly management can reduce morbidity and mortality.
HIT there is no diagnosis method at present.But currently used method is to carry out 4TS scorings (table 1) according to clinical manifestation to comment
Estimate Binding experiment room check exclude HIT negative patients, Warkentin by platelet count, time of origin, thrombus situation and
The concise 4Ts grade forms of hematoblastic four factor designs of possible cause, by constantly developing and numerous studies are verified, high probability
(6~8 points of scoring) positive predictive value is 0.64；Low probability (≤3 points of scoring) negative predictive value is 0.998.For tentatively excluding
Suspicious HIT is widely used in clinic, and obtains guide recommendation.
Since the current HIT positives make a definite diagnosis more difficulty, quickly, the PF4-H detections of high NPV exclude hand as negative
Duan Ze, which seems, to be even more important！
The HIT antibody test reagents that western countries use at present are mainly ELISA method, and this method is in external Clinical screening
In it is very universal, it is complicated for operation but due to the testing staff for needing profession, expend that the time is long, and positive predictive value is not high.Streaming is thin
Born of the same parents' art HITKit and SRA radioimmunoassays product is popularized because of clinical manipulation complexity, examining report overlong time, clinic
Degree is relatively low, and the immunoturbidimetry HIT-Ab (PF4-H) of HemosIL companies has good laboratory after clinical practice
Application value, but this method is large-scale Blood coagulation instrument detection project, is not suitable for POC by bed, clinic needs more convenient quickly
Method be detected, the STic Expert of Stago companiesWith the PIFA of Akers Biosciences companies
PlussPF4TMIt is detected available for clinical quick POCT, but HIT antibody is not qualitatively detected, clinical detection susceptibility is low.
Clinical limitation during heparin/PF4 antibody tests is carried out for above method, we provides a kind of new HIT
The Test paper of antibody, the test strip application fluorescent microsphere mark the particle of recombinant protein A, avoid conventional tag antibody
Unstability, improve detection sensitivity, HIT antibody in blood sample quantitatively detected by the way that lateral chromatography technology is immunized.
Invention content
(1) technical problems to be solved
It is accurately, reliably, quantitative the technical problem to be solved in the present invention is to provide sensitive, it is at low cost, quickly, hold
A kind of immune microsphere easily used chromatographs quick HIT antibody tests method for preparing test paper, and recombinant protein A is marked using fluorescent staining
Particle as instruction system, to replace colloid gold label, improve label detection stability, quantitatively detect heparin/PF4 in sample
Antibody, lateral immune chromatography method can be used for by the bed of patient detecting, and compensate for the limitation of test in laboratory.
(2) technical solution
In order to solve the above technical problem, the present invention provides a kind of immune microspheres to chromatograph quick HIT antibody tests test paper
Preparation method, Fig. 1 are the schematic diagram of the test strips of the present invention.
A kind of immune microsphere provided by the invention chromatographs quick HIT antibody tests method for preparing test paper and includes the following steps：
Position 3 shown in figure one is detection line, which is marked with heparin-PF4 compounds, and heparin-PF4 compounds are special
The opposite sex combines the mark substance of the HIT antibody and fluorescent staining microballoon in sample；
S1, heparin-PF4 compound preparation methods are as follows：
By the heparin preparations of buffer and 4 factor of blood platelet in room temperature condition according to volumes below than 1:1-1:5 but
It is not limited to the ratio and carries out mixing incubation, the glucide of 10%-50% is added in into reaction solution as stabilizer；
S2, albumin A particle preparation are selected but are not limited to fluorescent staining microballoon, latex beads, preparation are coated with albumin A use
In colour developing, convenient for instrument quantitative or qualitative detection heparin/PF4 antibody concentrations；
The preparation of S3, reaction film, it is characterised in that reacting pad includes but not limited to NC films, nitrocellulose membrane, acetate fiber
Film, nature controlling line and each one of detection line on reacting pad, detection line fixation are coated with S1 steps heparin/PF4 compounds, and nature controlling line is solid
Surely it is coated with human immunoglobulin(HIg) antibody.
Position 1 shown in figure one is sample pad area, region acceptor's sample, and sample is detached, and is allowed point
Blood plasma or serum sample from after are combined with the position 2 at one position of figure.
It is prepared by S4, sample pad, using but be not limited to glass fibre membrane, nitrocellulose filter, cellulose acetate film, NC films,
It is impregnated with one or more of preservatives, surfactant, seralbumin, sodium chloride, carbohydrate reagent, is placed in 37-56 DEG C
It is dried, is stored in sealing bags for use；
Position 2 shown in figure one is colour developing particle marker area, is made of tunica fibrosa, is marked with albumin A and dyed particles, should
Particle is excessive, is partly combined with the HIT antibody in proper manners sheet, passes through 3 region of position shown in lateral chromatography film flow graph one.
The sample pad of S5, particle marker, the albumin A particle examination in the 2 fiber membrane marker S2 of position of figure one after the drying
Agent is placed in 37-56 DEG C of drying process；
Position 1 shown in S6, figure one is blood separation membrane, will add in blood separation membrane in the sample pad of test-strips, is used for
Washed corpuscles allow blood plasma or serum after separation to enter sample pad；
S7, figure one show the overall schematic of test strips, and the assembling of test strips is mainly by the sample of S5 marker proteins A
This pad is assembled into viscose carrier plastics plate one end, is firmly pressed, and one kind is not limited to nitrocellulose filter or NC films assemble
In the middle part of test strips, the other end of test strips pastes absorbing membrane, maintains sample lateral chromatography, test strips are put into plastic casing,
Plastic box bottom is placed on by one section of sample pad, plastic casing top is fixed in blotting paper one end；
Clinical sample detects, and adds in whole blood sample, reaction at the sample pad position of test strips at room temperature；
Wherein in step sl prepare heparin-PF4 compounds, the buffer solution of heparin preparations and platelet factor 4 includes
But it is not limited to the buffer solution of the routine such as phosphate, Tris, HEPES.
Wherein the mixed proportion of heparin preparations and platelet factor 4 includes but not limited to volume ratio 1 in step sl:1-1:
5 carry out hybrid reaction,
The glucide wherein added in step sl includes but not limited to add in the carbohydrates such as sucrose, trehalose, glucose,
Glucide additional proportion includes but not limited to 10%-50%.
The colour developing particle of albumin A particle preparation wherein in step s 2 includes but not limited to fluorescent microsphere, dyed microspheres.
Albumin A marking particle method wherein described in step S2, which is characterized in that microballoon or colour developing grain diameter include but
It is not limited to 0.1-1 microns.
Colour developing particle marker wherein described in step S2 is recombinant protein A marking particle.
Detection line wherein described in step S3 is marked with heparin/PF4 compounds in S1 steps.
Sample pad reagent treatment surfactant in wherein step S4 includes but not limited to tween, Qula is led to, serum
Albumin includes but not limited to bovine serum albumin(BSA), human serum albumins etc., and glucide includes but not limited to add in sucrose, sea
The carbohydrates such as algae sugar, glucose,
Preparation method wherein described in step S7, it is characterised in that detection line is distributed on the cellulose membrane in the middle part of test strips
And nature controlling line.
Nature controlling line wherein described in step S7 is marked with recombinant protein A antibody, the antibody specificity combination recombinant protein A mark
Remember particle.
Test sample wherein described in step S7 is whole blood or ingredient blood.
HIT antibody behaviour immune globulin antibodies wherein in test sample, Antibody types include IgA, IgG, IgE, IgM
Antibody.
Wherein preservative includes but not limited to the biological preservatives such as Sodium azide, thimerosal, gentamicin sulphate in sample pad.
(3) advantageous effect
The above-mentioned technical proposal of the present invention has the advantages that：The present invention chromatographs quick HIT for a kind of immune microsphere
The preparation method of antibody test test paper, using the fluorescence or dyed microspheres of albumin A label as instruction system, dyed microspheres are total to
Valency binding protein A and HIT antibody with the conventional colloidal gold qualitative detection HIT antibody methods of substitution, solves the clinic of HIT antibody
Quantitative detection, improves sensitivity, stability that the colloidal gold on existing market detects, and accuracy is examined by POCT optical density
It surveys instrument and quantitatively detects HIT antibody, prompt weak, strong, the stronger graded of antibody titer, it is easy to operate when making clinical examination, it saves
When, sample dosage is few, generally only needs 50ul clinical samples, and testing result is clearly accurate, and repeatability is strong, testing result
Achieve the effect that naked eyes and the accurate interpretation of automatic machinery simultaneously, testing result is easy to standardize.Carrying out HIT antibody inspections simultaneously
Test paper increases control nature controlling line when testing, and ensures validity and the quality control of experimental result.
Description of the drawings
Fig. 1 is the schematic diagram that a kind of immune microsphere of the embodiment of the present invention chromatographs quick HIT antibody tests test paper；
Fig. 2 is implementation steps flow chart of the present invention
Specific embodiment
Embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples.Following embodiment is used for
Illustrate the present invention, but cannot be used for limiting the scope of the invention.
In the description of the present invention, unless otherwise indicated, " multiple " are meant that two or more.Term " on ",
" under ", "left", "right", " interior ", " outer ", " front end ", " rear end ", " head ", the orientation of the instructions such as " tail portion " or position relationship be
Based on orientation shown in the drawings or position relationship, it is for only for ease of the description present invention and simplifies description rather than instruction or dark
Show that signified device or element there must be specific orientation, with specific azimuth configuration and operation, therefore it is not intended that right
The limitation of the present invention.In addition, term " first ", " second ", " third " etc. be only used for description purpose, and it is not intended that instruction or
Imply relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or be integrally connected；It can
To be mechanical connection or be electrically connected；It can be directly connected, can also be indirectly connected by intermediary.For this
For the those of ordinary skill in field, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
Embodiment one
As shown in Figure 1, a kind of immune microsphere chromatographs quick HIT antibody tests method for preparing test paper, include the following steps：
S1, heparin/PF4 compounds prepare specific steps：
The heparin (1000U/ml) of 40ul is dissolved with the PBS buffer solution of PH7.4, and the blood for mixing the 1.4mg/ml of 94ul is small
The plate factor 4 adds in 56ul pure water, is incubated at room temperature the composite solution turned yellow after 30min, then adds in 50% sugarcane of 4ul
25% aqueous trehalose of sugar juice and 4ul, the TAPS buffer solutions that the 1M of the PH9.0 of 2ul is added in after mixing are for use.
Embodiment two
S2, albumin A particle preparation
Specific steps：The fluorescent microsphere of the 0.3um of 100ml 10% is taken, is washed 3 times with the MES solution of PH4.5 50mM,
The EDAC solution (MES of PH4.5 50mM is prepared) of the 10mg/ml of 1ml Fresh is added in, is incubated at room temperature 30min, Ran Houyong
2000rpm centrifuges 5min, is then washed with the MES solution of PH4.5 50mM, with the recombinant protein A of the 0.65mg/ml of 1ml
(PBS) incubation at room temperature overnight, is incubated at room temperature 1 hour with 10%BSA, 100 × Tris-EDTA, 3 is washed with 1mlPBS after centrifugation
It is secondary, in 4% sucrose of storage, 0.05% sodium azide solution.
Embodiment three
The preparation of S3, reaction film
Compound is sprayed to the nitrocellulose filter of Millipore Hi-Flowmylar backings with 0.075 μ l/mm
On (25mm wide, SHF0900425), spray to form p-wire with the constant speed of 45mm/s.Meanwhile 0.25mg/mL will be included and recombinate egg
The reference reagent of white A is mixed with 1% sucrose/0.5% trehalose/10mM TAPS pH 9.0, with 45mm/ seconds in 0.075 μ l/mm
Lower spraying nature controlling line.Then film is toasted one week at 56 DEG C.
Example IV
It is prepared by S4, sample pad
The sample pad of S5, dyed particles label
Specific steps：The membrane derived Millipore of glass fibre, with the ethylenediamine tetraacetic (propoxylation-embedding for containing 1% of mixing
Section-ethoxylate) tetrol surfactant, 0.8% casein and 5 × Tris-EDTA immersions, 56 DEG C of dryings, drying
Afterwards, by recombinant protein A particle of the volume of mixture than 2.5%, 5% sucrose, 2.5% trehalose, 5 × Tris-EDTA make
With professional spray gun, albumin A particle, 56 DEG C of dried for standby are sprayed in 75mm seconds with speed in height 5-7mm.
Embodiment five
S6, blood separation membrane assembling,
S7, test strips assembling
Specific steps：The high-quality glass fibre membrane BT100 of Millipore is chosen as sample cushion material, cut into 30 ×
2cm sizes are spare, choose 30 × 2cm blotting papers, and specification is the PVC plastic flitch of 30 × 6cm, is stained with double faced adhesive tape, then by NC films, sample
Product pad, blotting paper are pasted in PVC board, and the lap of splice between the film of each specification is no more than 0.5mm, convenient for lateral chromatography,
The test strips of 4mm wide are cut into after assembling, in packing and aluminium foil bag, 4 DEG C of storages.
In conclusion the present invention provides a kind of immune microspheres to chromatograph quick HIT antibody tests method for preparing test paper, the party
The fluorescence or dyed microspheres of method application albumin A label are as instruction system, the covalent bond albumin A of microballoon and HIT antibody, to take
Generation conventional colloidal gold qualitative detection HIT antibody methods, the clinic for solving HIT antibody are quantitatively detected, are improved on existing market
Colloidal gold detection sensitivity, stability, accuracy, pass through POC instrument quantitatives detect HIT antibody titre, prompt antibody
Weak, stronger, the strong graded of titre, easy to operate when making clinical examination, time saving, sample dosage is few, generally only needs 50ul
Clinical samples, testing result is clearly accurate, and repeatability is strong, and testing result reaches naked eyes simultaneously and automatic machinery is accurate
The effect of interpretation, testing result are easy to standardize.Simultaneously control Quality Control is increased when carrying out the experiment of HIT antibody tests test paper
Line ensures validity and the quality control of experimental result.
The embodiment of the present invention provides for the sake of example and description, and is not exhaustively or by this to send out
It is bright to be limited to disclosed form.Many modifications and variations are obvious for the ordinary skill in the art.Choosing
It is to more preferably illustrate the principle of the present invention and practical application to select and describe embodiment, and makes those of ordinary skill in the art
It will be appreciated that the present invention is so as to design the various embodiments with various modifications suitable for special-purpose.
Claims (7)
1. a kind of immune microsphere chromatographs quick HIT antibody tests test paper, can be heparin-induced in clinical assisted diagnosis patient
Thrombopenia antibody, include the use of that instant immunochromatography is quantitative or qualitative determination patient in heparin-PF4 compounds induce
Whether the body fluid levels of immune globulin antibody are higher than preset range, which is characterized in that include the following steps：
S1, heparin-PF4 compounds are prepared, by the heparin preparations of buffer and 4 factor of blood platelet room temperature condition according to
Lower volume is than 1:1-1:It 5 but is not limited to the ratio and carries out mixing incubation, the glucide that 10%-50% is added in into reaction solution is made
For stabilizer；
S2, albumin A particle preparation, the latex beads selected but be not limited to after dyeing, fluorescent microsphere, preparation are coated with albumin A use
In colour developing, convenient for instrument quantitative or qualitative detection heparin/PF4 antibody concentrations；
The preparation of S3, reaction film, it is characterised in that reacting pad includes but not limited to NC films, nitrocellulose membrane, cellulose acetate film, instead
Nature controlling line and each one of detection line should be padded, detection line fixation is coated with S1 steps heparin/PF4 compounds, and nature controlling line fixes spray
It is coated with human immunoglobulin(HIg) antibody.
S4, sample pad prepare, using but be not limited to glass fibre membrane, nitrocellulose filter, cellulose acetate film, NC films, with one
Kind or several preservatives, surfactant, seralbumin, sodium chloride, carbohydrate reagent are impregnated, and are placed in 37-56 DEG C of drying
Processing is stored in sealing bags for use；
The sample pad of S5, particle marker, the albumin A particle reagents in S4 sample pad proximal markers S2 after the drying, are placed in 37-
56 DEG C of drying process；
S6, blood separation membrane assembling, will add in blood separation membrane in the sample pad of test-strips, for washed corpuscles, allow point
Blood plasma or serum from after enter sample pad；
The sample pad of S5 marker proteins A is assembled into viscose carrier plastics plate one end, firmly by the assembling of S7, test strips
One kind is not limited to nitrocellulose filter or NC films is assembled in the middle part of test strips by pressing, and the other end of test strips pastes absorbing membrane,
Sample lateral chromatography is maintained, test strips are put into plastic casing, plastic box bottom, blotting paper one are placed on by one section of sample pad
Plastic casing top is fixed at end；
Clinical sample detects, and adds in whole blood sample, reaction at the sample pad position of test strips at room temperature；
In step sl prepare heparin-PF4 compounds, the buffer solution of heparin preparations and platelet factor 4 includes but not limited to
The buffer solution of the routine such as phosphate, Tris, HEPES.
The mixed proportion of heparin preparations and platelet factor 4 includes but not limited to volume ratio 1 in step sl:1-1:5 are mixed
Close reaction,
The glucide added in step sl includes but not limited to add in the carbohydrates such as sucrose, trehalose, glucose, glucide
Additional proportion includes but not limited to 10%-50%.
The colour developing particle of albumin A particle preparation in step s 2 includes but not limited to dyed microspheres, fluorescent microsphere.
Sample pad reagent treatment surfactant in step s3 includes but not limited to tween, Qula is led to, seralbumin
Including but not limited to bovine serum albumin(BSA), human serum albumins etc., glucide include but not limited to add in sucrose, trehalose,
The carbohydrates such as glucose.
2. test sample according to claim 1 is whole blood or ingredient blood.
3. a kind of immune microsphere according to claim 1 chromatographs quick HIT antibody tests method for preparing test paper, feature exists
In microballoon or colour developing grain diameter include but not limited to 0.1-1 microns.
4. preparation method according to claim 1, it is characterised in that detection is distributed on the cellulose membrane in the middle part of test strips
Line and nature controlling line.
5. colour developing particle marker according to claim 1 is recombinant protein A marking particle.
6. detection line according to claim 4 is marked with heparin/PF4 compounds in S1 steps.
7. nature controlling line according to claim 4 is marked with recombinant protein A antibody, the antibody specificity combination recombinant protein A
Marking particle.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103344771A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院苏州生物医学工程技术研究所 | Immunofluorescent chromatographic test strip and detection method for HIT (Heparin-induced thrombocytopenia) antibodies |
| CN103558381A (en) * | 2013-11-06 | 2014-02-05 | 昆明云大生物技术有限公司 | Immunochromatographic test paper for detecting human immunodeficiency virus antibodies and preparation method thereof |
| CN103728447A (en) * | 2014-01-03 | 2014-04-16 | 北京晶泰美康生物科技有限公司 | Controllable quantum dot locus specificity bridging coupling antibody marking method and application |
| CN103926400A (en) * | 2014-04-06 | 2014-07-16 | 邵超鹏 | Test strip for specifically measuring IgM, IgG and IgA blood group antibodies |
| CN104407134A (en) * | 2014-11-03 | 2015-03-11 | 清华大学深圳研究生院 | Method and kit for detecting A type influenza virus H1 subtype |
-
2017
- 2017-01-03 CN CN201710000252.8A patent/CN108267591A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103344771A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院苏州生物医学工程技术研究所 | Immunofluorescent chromatographic test strip and detection method for HIT (Heparin-induced thrombocytopenia) antibodies |
| CN103558381A (en) * | 2013-11-06 | 2014-02-05 | 昆明云大生物技术有限公司 | Immunochromatographic test paper for detecting human immunodeficiency virus antibodies and preparation method thereof |
| CN103728447A (en) * | 2014-01-03 | 2014-04-16 | 北京晶泰美康生物科技有限公司 | Controllable quantum dot locus specificity bridging coupling antibody marking method and application |
| CN103926400A (en) * | 2014-04-06 | 2014-07-16 | 邵超鹏 | Test strip for specifically measuring IgM, IgG and IgA blood group antibodies |
| CN104407134A (en) * | 2014-11-03 | 2015-03-11 | 清华大学深圳研究生院 | Method and kit for detecting A type influenza virus H1 subtype |
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