CN108265010A - A kind of method using aspergillus sydowii joint Indian mustard cadmium pollution soil repair - Google Patents
A kind of method using aspergillus sydowii joint Indian mustard cadmium pollution soil repair Download PDFInfo
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- CN108265010A CN108265010A CN201810252492.1A CN201810252492A CN108265010A CN 108265010 A CN108265010 A CN 108265010A CN 201810252492 A CN201810252492 A CN 201810252492A CN 108265010 A CN108265010 A CN 108265010A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C12R2001/66—Aspergillus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
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Abstract
The present invention relates to a kind of methods using aspergillus sydowii joint Indian mustard cadmium pollution soil repair, belong to microorganisms technical field.The aspergillus sydowii (Aspergillus sydowii) of the present invention is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center in February, 2018 No. 05, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and deposit number is CGMCC No.15385.The present invention provides a kind of methods using aspergillus sydowii joint Indian mustard cadmium pollution soil repair, have economical and efficient, the advantage for not causing secondary pollution, beautification landscape etc. by the method remediating heavy metal cadmium pollution soil.
Description
Technical field
The present invention relates to a kind of methods using aspergillus sydowii joint Indian mustard cadmium pollution soil repair, belong to micro- life
Object technical field.
Background technology
Since modern times, with industry and the rapid development of agricultural, heavy metal is also used more and more widely, therewith
The pollution problem come also more highlights.Receiving place of the soil as heavy metal contaminants maximum so that heavy metal pollution of soil
As one of global environmental problem.Therefore, prevention is badly in need of in the pollution of heavy metal pollution of soil, especially farmland soil heavy metals
With reparation.
The source of cadmium is mainly the exploitation of lead cadmium ore deposit and smelting and the discharge of electroplating industry Industrial " three Waste " in soil.
At present, Chinese arable land is accounted for about the 1/5 of total cultivated area by the area of the heavy metal pollutions such as cadmium, cadmium.Cadmium can excessively cause in soil
Soil degradation influences plant growth, causes crop failure, and cadmium is by can be by food chain finally in human body inner product after plants enriched
It is tired, human health is endangered, causes the symptoms of digestive tract such as mankind's vomiting, diarrhea, apocleisis, and cause the other symptoms such as mankind's anaemia.
Therefore, soil quality is the first line of defence of crop production safety, carries out the prevention of heavy metal in soil pollution
Have great importance with repair.However, heavy metal pollution in soil have latency, irreversibility, chronicity with
And popularity, and cannot be removed by biodegradation, it can only occur to mutually convert between position migration and various forms.
At present, three kinds of Physical, chemical method and bioanalysis are mainly included for the restorative procedure of heavy-metal contaminated soil.It passes
The physical chemistry restorative procedure of system adds modifying agent, inhibitor etc. by various physical processes or into soil and realizes weight in soil
The removal or passivation of metal, including heavy metal pollution of soil, Soil leaching, electro reclamation, electric heating reparation, stabilisation/curing etc., these skills
Not only cost is higher for art, destroys soil texture, easily causes soil fertility decline, it is also possible to lead to secondary pollution.
In recent years, study more bioremediation technology because its small investment, it is at low cost, to soil environment disturb it is small, do not draw
Rise secondary pollution the features such as and be concerned, wherein using plant absorption heavy metal be considered as solve heavy metal pollution of soil
One of ideal method, Indian mustard are exactly one of which hyperaccumulative plant, can absorb, are enriched with and shift heavy metal cadmium, be one
Plant resources of the kind with good repair ability.
But the plant available for geobiont repair having now been found that is limited by geographical conditions, weather conditions,
Usual biomass is smaller, slow-growing, thus leverages the potentiality of their restoration of soil polluted by heavy metal.
Invention content
The present invention provides a kind of methods using aspergillus sydowii joint Indian mustard cadmium pollution soil repair, pass through this
Method remediating heavy metal cadmium pollution soil has economical and efficient, the advantage for not causing secondary pollution, beautification landscape etc..The present invention is logical
It crosses and Indian mustard is planted in cadmium pollution soil, and in aspergillus sydowii of its root inoculation available for cadmium pollution soil repair
(Aspergillussydowii) so that heavy metal cadmium is enriched in Indian mustard, after Indian mustard maturation, by India
Leaf mustard integrally removes, so as to fulfill the purpose of the heavy metal cadmium object in removal soil.
Technical scheme is as follows：
The present invention provides a kind of aspergillus sydowii (Aspergillus sydowii) for cadmium pollution soil repair,
The aspergillus sydowii (Aspergillus sydowii) is preserved in Chinese microorganism strain preservation pipe in February, 2018 No. 05
Reason committee common micro-organisms center, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, deposit number CGMCC
No.15385。
The present invention provides the above-mentioned aspergillus sydowiis (Aspergillus sydowii) for cadmium pollution soil repair to exist
Administer the application in terms of cadmium pollution.
The present invention provides a kind of methods using aspergillus sydowii cadmium pollution soil repair, comprise the following steps：
Step 1：Indian mustard is planted in cadmium pollution soil；
Step 2：Resistance to cadmium microorganism is inoculated in Indian mustard root system；
Step 3：The operation of steps 1 and 2 is repeated after a collection of Indian mustard growth and maturity, until the cadmium content in soil is low
In 0.2mg/kg；
The resistance to cadmium microorganism is aspergillus sydowii (Aspergillus sydowii)；The aspergillus sydowii
It is commonly micro- that (Aspergillus sydowii) in February, 2018 No. 05 is preserved in China Committee for Culture Collection of Microorganisms
Bio-Centers, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and deposit number is CGMCC No.15385.
In one embodiment of the invention, the Indian mustard is planted in cadmium pollution soil, control soil contains
Water rate is maintained at 55-65%.
In one embodiment of the invention, the Indian mustard is planted in cadmium pollution soil, planting patterns is
Outdoor cultivation.
In one embodiment of the invention, the inoculation be Indian mustard root system inoculum density not less than 1 ×
106The aspergillus sydowii of CFU/g.
In one embodiment of the invention, it is described inoculation be by aspergillus sydowii strain be prepared into a concentration of 1-4 ×
108The bacteria suspension of CFU/mL sprays bacteria suspension before Indian mustard sowing with the 2nd, 4,6 week after emergence respectively.
In one embodiment of the invention, it is described inoculation be by aspergillus sydowii strain be prepared into a concentration of 1-4 ×
108The bacteria suspension of CFU/mL sprays bacteria suspension 5mL before Indian mustard sowing with the 2nd, 4,6 week after emergence respectively.
In one embodiment of the invention, the sprinkling bacteria suspension is that bacteria suspension is sprayed onto Indian mustard surface.
The present invention provides the above-mentioned methods using aspergillus sydowii joint Indian mustard cadmium pollution soil repair to administer
Application in terms of cadmium pollution.
Advantageous effect：
(1) this aspergillus sydowii (Aspergillus sydowii) provided by the invention has height endurability to cadmium,
Cd2+Minimum inhibitory concentration (MIC) to the bacterium is 1500mg/L；
(2) Indian mustard can be promoted to give birth to this aspergillus sydowii (Aspergillus sydowii) provided by the invention
The increase of object amount after the bacterium of the present invention is inoculated in Indian mustard root system, may be such that Indian mustard overground part fresh weight and dry weight are shown
Writing increases 14%-125% and 7%-134%, and underground part fresh weight and dry weight dramatically increase 12%-63% and 21%-82%；
(3) this method cadmium pollution soil repair provided by the invention is used, Indian mustard can be remarkably promoted in soil
The enrichment and absorption of heavy metal cadmium, improve extraction effect of the Indian mustard to cadmium, and the bacterium of the present invention is inoculated in Indian mustard root
After system, it may be such that soil available Cd concentration highest improves 36%, simultaneously so that overground part cadmium concentration improves in Indian mustard
4%-36%, underground part cadmium concentration improve 23%-36%；
(4) method of this cadmium pollution soil repair provided by the invention has that practicality is strong, highly effective and safe, does not destroy soil
Earth structure and property, the advantages that not causing secondary pollution, beautifying the environment, application prospect is extensive.
Biomaterial preservation
A kind of aspergillus sydowii (Aspergillus sydowii), Classification And Nomenclature is：Aspergillus sydowii, depositary institution are：In
State's Microbiological Culture Collection administration committee common micro-organisms center, preservation address are：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
No. 3, deposit number is：CGMCC No.15385, preservation date are：On February 05th, 2018.
Description of the drawings
Influence of the bacterial strain of the present invention to Indian mustard biomass under Fig. 1-4 difference cadmium concentrations.
Influence of the bacterial strain of the present invention to soil available Cd concentration under Fig. 5 difference cadmium concentrations.
Fig. 6-7 is inoculated with influence of the bacterial strain of the present invention to Indian mustard cadmium concentration.
Fig. 8 is inoculated with the influence of bacterial strain total Cd uptake amount to Indian mustard of the present invention.
Specific embodiment
The following examples of the present invention are only as the further explanation of the content of present invention, it is impossible in the restriction as the present invention
Perhaps range.
The detection method of Indian mustard biomass：
Mixed sampling is carried out per basin plant, is cut along native face, is divided into two parts above and below the ground, distilled water is cleaned and uses suction
Water paper blots, and with electronic balance weighing fresh weight, is then placed into water-removing 0.5h in 105 DEG C of baking ovens, 65 DEG C dry to constant weight, electronics day
It is flat to weigh dry weight.
The detection method of soil available Cd content：
After potted plant sampling, take the pedotheque around primitive root border, 60 mesh nylon mesh are crossed after air-drying, by native liquor ratio 1:
10 ratio adds in 0.1mol/LHCl extractants, and the available Cd in soil is measured using flame atomic absorption spectrophotometer
Content.The detection method of Indian mustard Cd uptake amount：
Each section sample comminution after drying, respectively takes 0.2g samples, adds in nitric acid and perchloric acid (4:1, v:V) mixed acid,
Micro-wave digestion is carried out, the Cd contents of part above and below the ground are measured using flame atomic absorption spectrophotometer.
The detection method of the total Cd uptake amount of Indian mustard：
Plant parts cadmium content, which is multiplied by plant parts dry weight and is added again, can obtain total Cd uptake amount.
Bacterial strain in following embodiments is the aspergillus sydowii (Aspergillus screened from cadmium pollution soil
Sydowii), this aspergillus sydowii (Aspergillus sydowii) is preserved in China Microbiological in February, 2018 No. 05
Culture presevation administration committee common micro-organisms center, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, deposit number
For CGMCCNo.15385.
Embodiment
Specific implementation step is as follows：
(1) be separately added into 0 in the soil, 0.0856,0.1713, the CdCl of 0.3425g2Solid powder adds water stirring mixed
It is even, soil metal Cd concentration is made to be respectively 0,28,56,112mg/kg, each concentration sets 3 pottings, is finally averaged, mould
Intend Cd contaminated soils；
(2) soil stabilization cools down laggard luggage basin, Cd contaminated soils is packed into per basin after 121 DEG C of high-temperature sterilization 20min
1.5kg, each concentration set 6 basins respectively as bacterium group and control group is connect, totally 24 basin；
(3) bacterium group and control group are being connect using direct-seeding sowing Indian mustard seed respectively, 8 seeds of sowing per basin,
1 week after germination, thinning is carried out, 5 plants of seedling that reservation is grown fine per basin；
(4) the 2nd, 4,6 week a concentration of 1-4 × 10 of sprinkling 5mL after preceding and emergence need to be sowed by connecing the potting of bacterium group8CFU/mL's
Aspergillus sydowii (Aspergillus sydowii) bacteria suspension, control group potting add in equivalent sterile water every time；
(5) during plant strain growth, gravity-flow ventilation is kept, has enough illumination, waters sooner or later daily, ensure that soil is wet
Profit, control soil moisture content are maintained at 55-65%, plant are gathered in after Indian mustard maturation.
The testing result of Indian mustard biomass is as follows：
Under different soils Cd concentration, the visible Fig. 1-4 of the influence of aspergillus sydowii of the invention to Indian mustard biomass.
From Fig. 1,2：Compared with not connecing bacterium group, bacterium group Indian mustard is connect in the soil of 0,28,56 and 112mg/kg of Cd concentration
Top fresh weight difference amount of increase 125%, 122%, 41%, 14%, underground part fresh weight difference amount of increase 25%, 54%, 12%, 63%.
From Fig. 3,4：The overground part dry weight ratio that bacterium group plant is connect in the soil of 0,28,56 and 112mg/kg of Cd concentration does not connect bacterium group
134%, 115%, 39%, 7% is respectively increased, underground part dry weight is respectively increased 52%, 82%, 31%, 21%.
To sum up the result shows that, compared with the control group, aspergillus sydowii of the invention increases the matter of Indian mustard each section
Amount, especially in the soil of 0 and 28mg/kgCd concentration, facilitation effect is more notable.
The testing result of soil available Cd content is as follows：
Under different soils cadmium concentration, it is inoculated with influence visible figure of the aspergillus sydowii of the present invention to effective cadmium content of soil
5.As shown in Figure 5：Under 28,56 and 112mg/kg metal concentrations, the soil available cadmium concentration for connecing bacterium group is more other than control component
Increase 36%, 27%, 4%.
Metallic element in soil exists with different chemical forms, and the metallic element of different chemical forms is only converted to
Available state could be enriched with by plant.Available state tenor can evaluate the environmental risk of contaminated soil, biological effectiveness and
Toxicity, therefore study metal available state and will be seen that soil pollution situation.In the present embodiment, it is inoculated with the aspergillus sydowii of the present invention
Effective cadmium content of soil is improved, under 28mg/kg Cadmium treateds, the soil available cadmium concentration ratio for connecing bacterium group does not connect bacterium group
Highest improves 36%, illustrates the aspergillus sydowii of the present invention and can dissolve insoluble Cd in the soil, improves soil available cadmium
Content.
The testing result of Indian mustard Cd uptake amount is as follows：
The visible Fig. 6-7 of influence of the aspergillus sydowii of the present invention to Indian mustard Cd uptake is inoculated under different cadmium concentrations.
As shown in Figure 6：Soil Cd it is a concentration of 28,56,112mg/kg when, compared with the control group, connect the Indian mustard overground part of bacterium group
Cd concentration is respectively increased 4%, 16%, 26%；As shown in Figure 7：Soil Cd it is a concentration of 28,56,112mg/kg when, connect bacterium group
Indian mustard underground part Cd concentration significantly improves 33%, 36%, 23% compared with the control group.
It finds in this experiment, Indian mustard overground part and the underground part Cd concentration for being inoculated with the aspergillus sydowii of the present invention are equal
There is different degrees of increase, this may be because the aspergillus sydowii of the present invention promotes the dissolving of soil insoluble Cd, increase
Soil available Cd concentration improves Indian mustard and the absorption of cadmium is enriched with.
The testing result of the total Cd uptake amount of Indian mustard is as follows：
Visible Fig. 8 of influence of inoculation bacterial strain total Cd uptake amount to Indian mustard of the present invention.As shown in Figure 8：It is dense in different cadmiums
In the soil of degree, be inoculated with bacterial strain of the present invention the total Cd uptake amount of Indian mustard compare do not connect bacterium group be respectively increased 122%, 108%,
31%th, 46%, illustrate that bacterial strain of the present invention can significantly improve gross absorption of the Indian mustard to cadmium.
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not limited to the present invention, any to be familiar with this skill
The people of art without departing from the spirit and scope of the present invention, can do various change and modification, therefore the protection model of the present invention
Enclosing be subject to what claims were defined.
Claims (10)
1. a kind of aspergillus sydowii (Aspergillus sydowii) for cadmium pollution soil repair, which is characterized in that described
Aspergillus sydowii (Aspergillus sydowii) is preserved in Chinese microorganism strain preservation management committee in February, 2018 No. 05
Member can common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, deposit number CGMCC
No.15385。
2. a kind of aspergillus sydowii (Aspergillus for cadmium pollution soil repair as described in claim 1
Sydowii) the application in terms of cadmium pollution is administered.
A kind of 3. method using aspergillus sydowii joint Indian mustard cadmium pollution soil repair, which is characterized in that comprising as follows
Step：
Step 1：Indian mustard is planted in cadmium pollution soil；
Step 2：Resistance to cadmium microorganism is inoculated in Indian mustard root system；
Step 3：The operation of steps 1 and 2 is repeated after a collection of Indian mustard growth and maturity, until the cadmium content in soil is less than
0.2mg/kg；
The resistance to cadmium microorganism is aspergillus sydowii (Aspergillus sydowii)；The aspergillus sydowii
It is commonly micro- that (Aspergillus sydowii) in February, 2018 No. 05 is preserved in China Committee for Culture Collection of Microorganisms
Bio-Centers, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and deposit number is CGMCC No.15385.
4. a kind of method using aspergillus sydowii joint Indian mustard cadmium pollution soil repair as claimed in claim 3,
It is characterized in that, the Indian mustard is planted in cadmium pollution soil, control soil moisture content is maintained at 55-65%.
5. a kind of method using aspergillus sydowii joint Indian mustard cadmium pollution soil repair as claimed in claim 4,
It is characterized in that, the Indian mustard is planted in cadmium pollution soil, planting patterns is outdoor cultivation.
6. a kind of side using aspergillus sydowii joint Indian mustard cadmium pollution soil repair as described in claim 3-5 is any
Method, which is characterized in that the inoculation is to be not less than 1 × 10 in Indian mustard root system inoculum density6The aspergillus sydowii of CFU/g.
7. a kind of side using aspergillus sydowii joint Indian mustard cadmium pollution soil repair as described in claim 3-6 is any
Method, which is characterized in that the inoculation is that aspergillus sydowii strain is prepared into a concentration of 1-4 × 108The bacteria suspension of CFU/mL, point
The the 2nd, 4,6 week sprinkling bacteria suspension not before Indian mustard sowing and after emergence.
8. a kind of side using aspergillus sydowii joint Indian mustard cadmium pollution soil repair as described in claim 3-7 is any
Method, which is characterized in that the inoculation is that aspergillus sydowii strain is prepared into a concentration of 1-4 × 108The bacteria suspension of CFU/mL, point
The the 2nd, 4,6 week sprinkling bacteria suspension 5mL not before Indian mustard sowing and after emergence.
9. a kind of method using aspergillus sydowii joint Indian mustard cadmium pollution soil repair as claimed in claim 7 or 8,
It is characterized in that, the sprinkling bacteria suspension is that bacteria suspension is sprayed onto Indian mustard surface.
10. combining Indian mustard cadmium pollution soil repair using aspergillus sydowii such as a kind of as described in claim 3-9 is any
Application of the method in terms of cadmium pollution is administered.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810252492.1A CN108265010B (en) | 2018-03-26 | 2018-03-26 | Method for repairing cadmium-polluted soil by using aspergillus versicolor and Indian mustard |
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| CN201810252492.1A CN108265010B (en) | 2018-03-26 | 2018-03-26 | Method for repairing cadmium-polluted soil by using aspergillus versicolor and Indian mustard |
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| CN108265010A true CN108265010A (en) | 2018-07-10 |
| CN108265010B CN108265010B (en) | 2020-03-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114602968A (en) * | 2022-04-14 | 2022-06-10 | 浙江树人学院 | Method for enhancing phytoremediation efficiency of heavy metal contaminated soil through synergism of nitrogen fertilizer and microbial inoculum |
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| CN102994398A (en) * | 2012-12-11 | 2013-03-27 | 四川绵竹剑南春酒厂有限公司 | Urease high-yielding Aspergillus sydowii and application thereof |
| CN105602855A (en) * | 2015-11-09 | 2016-05-25 | 武汉大学 | Aspergillus sydowii with broad-spectrum pesticide degradation characteristics |
| CN105290103A (en) * | 2015-11-23 | 2016-02-03 | 湖南农业大学 | Method for utilizing cadmium-resisting fungus aspergillus aculeatus for promoting festuca arundinacea to remedy cadmium contaminated soil |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114602968A (en) * | 2022-04-14 | 2022-06-10 | 浙江树人学院 | Method for enhancing phytoremediation efficiency of heavy metal contaminated soil through synergism of nitrogen fertilizer and microbial inoculum |
| CN114602968B (en) * | 2022-04-14 | 2023-04-07 | 浙江树人学院 | Method for enhancing phytoremediation efficiency of heavy metal contaminated soil through synergism of nitrogen fertilizer and microbial inoculum |
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| CN108265010B (en) | 2020-03-06 |
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