CN108250282A - The extracting method of fibroin and the suture containing fibroin - Google Patents
The extracting method of fibroin and the suture containing fibroin Download PDFInfo
- Publication number
- CN108250282A CN108250282A CN201711500671.4A CN201711500671A CN108250282A CN 108250282 A CN108250282 A CN 108250282A CN 201711500671 A CN201711500671 A CN 201711500671A CN 108250282 A CN108250282 A CN 108250282A
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- CN
- China
- Prior art keywords
- fibroin
- silk
- parts
- suture
- extracting method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L17/00—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
- A61L17/005—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters containing a biologically active substance, e.g. a medicament or a biocide
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L17/00—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
- A61L17/06—At least partially resorbable materials
- A61L17/10—At least partially resorbable materials containing macromolecular materials
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
- A61L2300/406—Antibiotics
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Vascular Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Surgery (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Insects & Arthropods (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Cosmetics (AREA)
- Peptides Or Proteins (AREA)
Abstract
Fibroin suture, in terms of mass fraction, including 30 40 parts of fibroin, 35 parts of roxithromycin, 35 parts of VC.Fibroin suture in the present invention is degradable material rich in fibroin extract, environmental protection simple for process, can slowly be absorbed by skin, it is not easy to scar.
Description
Technical field
The present invention relates to technical field of biological materials more particularly to fibroin extracting method and contain fibroin
Suture.
Background technology
Silk is one of native protein being utilized earliest, and human use's silk has as the history of fabric more than 5,000
Silk makes it in the great numbers technical field such as biological medicine because of its excellent mechanical property and good biocompatibility in recent years by year
It is paid more and more attention, fibroin albumen is also increasing as the report of operation suture thread, organizational project and Thermosensitive Material Used for Controlled Releasing of Medicine.
At present, degumming is a very important process in silk extraction protein processes, but the efficiency of degumming at present
Still it is very undesirable, seriously affect the purity of protein.
Invention content
In view of this, the object of the present invention is to provide a kind of extracting method of fibroin, so as to solve above-mentioned background
The problem of being proposed in technology.
The present invention solves above-mentioned technical problem by following technological means：
The extracting method of fibroin, includes the following steps：
S1：Silk is dissolved in degumming 30-40min in the boiling water of sodium bicarbonate.It is then placed in deep freezer and cools down rapidly
To -10~-15 DEG C；
S2：It is taken out from refrigerator and is gradually restored to room temperature, then washed to eliminate silk gum, 60~65 DEG C of bakings with massive laundering
It is dry；
S3：It is the semi-transparent of 12000~14000Da that mixed liquor, which is added in saturation ethanol solution, and uses molecular cut off
Film is dialysed 3d in deionized water；
S4：Complete solution dialyse through centrifuge, takes supernatant, it is dry by freezing to add in appropriate alcohols denaturant
It is dry to obtain perforated membrane；
S5：Perforated membrane in ethanol solution is subjected to different degrees of post processing, is lyophilized after deionized water washing and obtains silkworm
Silk-fibroin.
S6：The carbon nano-particle prepared is taken, is dissolved in deionized water, is stirred evenly, adds in lactic acid bacteria, stands 30min,
Fibroin is added in, stirs evenly, then dries, obtain degradable fibroin.
The present invention also provides the fibroin suture being process containing the above method, in terms of mass fraction, including
30-40 parts of fibroin, 3-5 parts of roxithromycin, 3-5 parts of VC.
Beneficial effects of the present invention：Fibroin suture in the present invention is being stitched rich in fibroin extract
The lactic acid bacteria carried by carbon nanoparticle is loaded in zygonema, is kept after it has been gathered, lactic acid bacteria is slowly released after being contacted with human skin
It releases, fibroin is decomposed, realize the ablation of suture, slowly absorbed by skin, it is not easy to scar.
Specific embodiment
The present invention is described in detail below：
Embodiment one
The extracting method of fibroin, includes the following steps：
S1：Silk is dissolved in degumming 40min in the boiling water of sodium bicarbonate.Be then placed in deep freezer be cooled to rapidly-
15℃；
S2：It is taken out from refrigerator and is gradually restored to room temperature, then washed to eliminate silk gum, 60 DEG C of drying with massive laundering；
S3：Mixed liquor is added in saturation ethanol solution and using the semi-permeable membrane that molecular cut off is 14000Da in going
Dialyse 3d in ionized water；
S4：Complete solution dialyse through centrifuge, takes supernatant, it is dry by freezing to add in appropriate alcohols denaturant
It is dry to obtain perforated membrane；
S5：Perforated membrane in ethanol solution is subjected to different degrees of post processing, is lyophilized after deionized water washing and obtains silkworm
Silk-fibroin.
S6：The carbon nano-particle prepared is taken, is dissolved in deionized water, is stirred evenly, adds in lactic acid bacteria, stands 30min,
Fibroin is added in, stirs evenly, then dries, obtain degradable fibroin..
Embodiment two
Fibroin suture, including fibroin 30g, roxithromycin 3g, VC 3g.
The above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to preferred embodiment to this hair
It is bright to be described in detail, it will be understood by those of ordinary skill in the art that, it can modify to technical scheme of the present invention
Or equivalent replacement, without departing from the objective and range of technical solution of the present invention, the claim in the present invention should all be covered
In range.
Claims (3)
1. the extracting method of fibroin, which is characterized in that include the following steps：
S1：Silk is dissolved in degumming 30-40min in the boiling water of sodium bicarbonate.It is then placed in deep freezer and is cooled to -10 rapidly
~-15 DEG C；
S2：It is taken out from refrigerator and is gradually restored to room temperature, then washed to eliminate silk gum, 60~65 DEG C of drying with massive laundering；
S3：By mixed liquor be added in saturation ethanol solution and using the semi-permeable membrane that molecular cut off is 12000~14000Da in
Dialyse 3d in deionized water；
S4：Complete solution dialyse through centrifuge, takes supernatant, adds in appropriate alcohols denaturant by being freeze-dried
To perforated membrane；
S5：Perforated membrane in ethanol solution is subjected to different degrees of post processing, is lyophilized after deionized water washing and obtains silk egg
In vain.
S6：The carbon nano-particle prepared is taken, is dissolved in deionized water, is stirred evenly, adds in lactic acid bacteria, stands 30min, is added in
Fibroin stirs evenly, and then dries, and obtains degradable fibroin.
2. fibroin suture made from the extracting method of fibroin as described in claim 1.
3. a kind of fibroin suture as claimed in claim 2, which is characterized in that in terms of mass fraction, including silk egg
30-40 parts white, 3-5 parts of roxithromycin, 3-5 parts of VC.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711500671.4A CN108250282A (en) | 2017-12-31 | 2017-12-31 | The extracting method of fibroin and the suture containing fibroin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711500671.4A CN108250282A (en) | 2017-12-31 | 2017-12-31 | The extracting method of fibroin and the suture containing fibroin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108250282A true CN108250282A (en) | 2018-07-06 |
Family
ID=62725797
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711500671.4A Pending CN108250282A (en) | 2017-12-31 | 2017-12-31 | The extracting method of fibroin and the suture containing fibroin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108250282A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113197705A (en) * | 2021-04-02 | 2021-08-03 | 苏州大学 | Barbed silk suture line and preparation method thereof |
-
2017
- 2017-12-31 CN CN201711500671.4A patent/CN108250282A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113197705A (en) * | 2021-04-02 | 2021-08-03 | 苏州大学 | Barbed silk suture line and preparation method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180706 |