CN108249564B - Preparation method and use method of denitrification solid carbon source - Google Patents
Preparation method and use method of denitrification solid carbon source Download PDFInfo
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 29
- 239000007787 solid Substances 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000010865 sewage Substances 0.000 claims abstract description 23
- 239000000463 material Substances 0.000 claims abstract description 12
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims abstract description 11
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims abstract description 11
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims abstract description 9
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims abstract description 9
- 239000011565 manganese chloride Substances 0.000 claims abstract description 9
- 235000002867 manganese chloride Nutrition 0.000 claims abstract description 9
- 229940099607 manganese chloride Drugs 0.000 claims abstract description 9
- 239000010802 sludge Substances 0.000 claims abstract description 9
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims abstract description 6
- 229920002498 Beta-glucan Polymers 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 5
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims abstract description 4
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 3
- 229920000520 poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Polymers 0.000 claims abstract 5
- 238000007599 discharging Methods 0.000 claims abstract 2
- 238000011049 filling Methods 0.000 claims description 17
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 6
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 5
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000014759 maintenance of location Effects 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 229940070710 valerate Drugs 0.000 claims description 3
- 150000002148 esters Chemical class 0.000 claims 2
- 239000000945 filler Substances 0.000 abstract description 18
- 244000005700 microbiome Species 0.000 abstract description 15
- 241000894006 Bacteria Species 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 4
- VGPSUIRIPDYGFV-UHFFFAOYSA-N [N].O[N+]([O-])=O Chemical compound [N].O[N+]([O-])=O VGPSUIRIPDYGFV-UHFFFAOYSA-N 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 239000002994 raw material Substances 0.000 description 5
- 229910002651 NO3 Inorganic materials 0.000 description 4
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- YNBJVVSSKUMOGH-UHFFFAOYSA-N 3-hydroxybutanoic acid;2-hydroxypentanoic acid Chemical compound CC(O)CC(O)=O.CCCC(O)C(O)=O YNBJVVSSKUMOGH-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000010355 oscillation Effects 0.000 description 3
- 230000002572 peristaltic effect Effects 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 239000011592 zinc chloride Substances 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000012237 artificial material Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000008235 industrial water Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920000070 poly-3-hydroxybutyrate Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- NQPDZGIKBAWPEJ-UHFFFAOYSA-N valeric acid Chemical compound CCCCC(O)=O NQPDZGIKBAWPEJ-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
A preparation method and a use method of a denitrification solid carbon source, belonging to water treatmentThe technical field is that the preparation method of the denitrification solid carbon source comprises the steps of adding sodium bicarbonate, anhydrous ferric chloride and anhydrous manganese chloride into molten PHBV according to a certain proportion, cooling to prepare a modified PHBV material, bagging a filler, then vibrating and pretreating the filler in 1-6% beta-glucan water solution, airing the filler and then putting the filler into a reactor for use. Injecting a water sample mixed with the prepared sewage and the sludge in the anaerobic tank into the reactor, continuously operating for 36-48 h, continuously operating by using the prepared sewage, and discharging NO after film formation is successful3 ‑The concentration of-N may be lower than 5 mg/L. The invention has the advantages that the microorganism attachment space is greatly increased, and the quantity of denitrifying bacteria is increased; the start-up time of the reactor is shortened; the wettability of the material is improved; the absorption of the microorganism to nutrient substances is accelerated, and the propagation of the microorganism is strengthened; the concentration of the effluent nitric acid nitrogen is stable and meets the sewage discharge requirement; the operation is simple and the control is easy.
Description
Technical Field
The invention belongs to the technical field of water treatment, and particularly relates to a preparation method and a use method of a denitrification solid carbon source. A modified poly-3-hydroxybutyrate-hydroxyvalerate (PHBV) by sodium bicarbonate, anhydrous ferric chloride and anhydrous manganese chloride is used as a denitrification device filler and a specific use method.
Background
In recent years, due to the change of economic industry of China, a large amount of nitrogenous domestic sewage and agricultural sewage are discharged into water bodies. The content of nitrate nitrogen in surface water systems of various regions and underground water of partial regions is increased sharply, and the method becomes a field needing to be developed intensively in the current water treatment technology.
At present, the main means for removing nitrate nitrogen in town sewage treatment is to convert nitrate nitrogen into nitrogen to be discharged into the atmosphere by the action of denitrifying bacteria. In order to be able to ensure sufficient activity of the denitrifying bacteria, it is necessary to have a sufficient carbon source as an energy supply in their reaction environment.
The existing sewage treatment process in China generally has the condition of insufficient carbon source in the denitrification link, and on the other hand, the discharge standard of total nitrogen is increasingly raised along with the improvement of the water treatment standard. Therefore, under the condition of ensuring the water conditions, the research on novel external carbon sources has become an urgent key problem in the water treatment process.
The existing external carbon sources can be generally divided into two major types, namely traditional soluble carbon sources and novel solid carbon sources (Shao stay, Xu Zu Xin, Yi sea dragon, research progress of external carbon sources in denitrification process of polluted water body, industrial water treatment 27(12) (2007) 10-14).
The traditional soluble carbon source mainly comprises liquid organic matters such as methanol, acetic acid, glucose and the like. However, such soluble organics generally have some problems, such as: the carbon-nitrogen ratio is difficult to control; the method has the operating problems that the nitrate nitrogen in water is difficult to regulate and control when fluctuation occurs, the COD (chemical oxygen demand) of effluent water quality is increased when excessive carbon source addition is carried out, and the generation of nitrite is accumulated when insufficient addition is carried out.
The above conditions can cause the quality of the effluent to be poor, and influence is caused. And the solid carbon source can be utilized by denitrifying bacteria only by decomposing the filler by using enzyme in the microorganism under the condition that the microorganism is attached. On one hand, the control problem of carbon source addition is avoided, and on the other hand, the effluent quality is not influenced.
In recent years, many researchers at home and abroad search for a nontoxic and cheap slow-release carbon source to replace the traditional carbon source through many ways, and most of the slow-release carbon source is natural solid organic matter rich in cellulose substances and some biodegradable artificial materials, such as cotton, rice hulls, paper, degradable meal boxes, PCL, starch and the like. However, such materials generally have the disadvantages of hydrophobicity, easy crystallization, long degradation time and the like.
Poly-3-hydroxybutyrate valerate (PHBV) is biological polyester which is produced by using starch as a raw material and applying a fermentation engineering technology. The application of the composite material in the technical field of water treatment has proved to be effective, but the material has the defects of stronger hydrophobicity, large particle density, difficulty in providing a microorganism living place and the like, and can influence the use in practical engineering. Therefore, the PHBV raw material is modified, and the problems are solved.
Disclosure of Invention
In order to solve the problems of long film forming time, small number of microorganisms per unit volume, low biological film strength, poor impact resistance and the like of the traditional solid carbon source, a preparation method and a use method of the denitrification solid carbon source are provided.
The specific scheme of the invention is as follows: a preparation method of a denitrification solid carbon source is characterized by comprising the following steps:
(1) putting poly (3-hydroxybutyrate-hydroxyvalerate) (PHBV) powder into a beaker, and heating for 3-5 min at 160-175 ℃; after the poly 3-hydroxybutyrate-hydroxyvalerate is melted, the mass ratio of poly 3-hydroxybutyrate-hydroxyvalerate: sodium bicarbonate: anhydrous ferric chloride: anhydrous manganese chloride = 100: 5-10: 0.005-0.020: adding 0.001-0.010 reagent into a beaker, and stirring for 30-60 s at the speed of 60-120 rpm/min to prepare the denitrification solid carbon source.
(2) Cooling the denitrification solid carbon source, taking out, and filling into a mesh-shaped filler bag with the aperture of 1.0-2.0 mm to obtain the filler porosity of 55-65%; placing the filling bag into a container filled with 1-6% of beta-glucan water solution, and oscillating for 15-30 s on a table oscillator; taking out the filling bag and drying in the air; putting the mixture into a reactor, wherein the filling rate of the reactor is 50%; the filler soaked by the beta-glucan aqueous solution can enhance the interception capability on microorganisms and improve the film hanging speed; through oscillation, the air content in the modified PHBV material is reduced, and the influence on an anaerobic environment can be reduced; the filling bag can be taken out at any time, so that the reactor and the filling are convenient to clean, replace and test, and the filling bag is prevented from floating upwards;
(3) mixing the prepared sewage (formula shown in table 1) and activated sludge according to the volume ratio of = 1: 3 and stirring at a speed of 60rpm/min to form a mixed liquid. KH in 50L sewage2PO4The content is 1.00 g; MgSO (MgSO)4The content is 0.55 g; CaCl2The content is 0.14 g; MnSO4The content is 0.13 g; FeCl3·6H2The O content is 0.18 g; ZnCl2The content is 0.11 g; NaNO3The content was 12.14 g.
(4) The mixed liquid enters from the lower part of the reactor through a peristaltic pump, flows out from the top and continuously runs for 36-48 h; then preparing sewage and continuously operating the reactor, keeping the hydraulic retention time at 1.5h, and carrying out rapid film formation; taking effluent liquid of the reactor every 24 hours, filtering, measuring the content of nitrate nitrogen and DOC and the pH value, and maintaining the operation of the reactor.
The activated sludge is from anaerobic pond sludge of a sewage treatment plant.
The modified PHBV material is used as a solid carbon source of a reactor and a microorganism attachment carrier. The method has the advantages that the microorganism attachment space is greatly increased, and the quantity of denitrifying bacteria is increased; the start-up time of the reactor is shortened; the wettability of the material is improved; the absorption of the microorganism to nutrient substances is accelerated, and the propagation of the microorganism is strengthened; the concentration of the effluent nitric acid nitrogen is stable and meets the sewage discharge requirement; the operation is simple and the control is easy.
The invention has the beneficial effects that:
(1) the modified PHBV has improved porosity and viscosity, greatly improves the attachment of microorganisms and improves the denitrification efficiency;
(2) the modified PHBV is more suitable for the survival of denitrifying microorganisms and the propagation of the microorganisms is strengthened;
(3) the modified PHBV greatly enhances the denitrification capability of the PHBV and shortens the starting time of the reactor;
(4) the modified PHBV filler does not have obvious influence on the pH environment in the reactor.
Drawings
FIG. 1 is a flow chart of the manufacturing process of the modified PHBV filler of the present invention.
FIG. 2 is a process flow diagram of a method of use of the present invention.
Reference numbers in the figures: 1-a water inlet barrel; 2-a peristaltic pump; 3-a water inlet; 4-a reactor; 5-filling mesh bags; 6-water outlet; 7-water outlet; 8-a filler; 9-a stirrer.
Detailed description of the preferred embodiments
The invention provides a method for modifying PHBV raw material and removing nitric acid and nitrogen in water by using the PHBV raw material as reactor filler, and the invention is further explained by combining the attached drawings and a specific embodiment. Wherein, the commercial PHBV, beta-glucan, laboratory sodium bicarbonate, anhydrous ferric chloride and anhydrous manganese chloride reagents are selected and analyzed to be pure.
In the invention, KH is contained in 50L of prepared sewage2PO4 1.00 g; MgSO (MgSO)40.55 g; CaCl20.14 g; MnSO40.13 g; FeCl3·6H2O is 0.18 g; ZnCl20.11 g; NaNO3It was 12.14 g.
The reactor of the invention is a packed bed reaction column.
Example 1
(1) Putting the PHBV powder into a beaker, and heating for 5min at 160-175 ℃. After the PHBV is melted, the mass ratio of PHBV: sodium bicarbonate: anhydrous ferric chloride: anhydrous manganese chloride = 100: 5: 0.010: adding 0.005 reagent into a beaker, stirring at the speed of 100rpm/min for 60s, and naturally cooling to prepare a filler;
(2) cooling the filler, taking out, filling the filler into a mesh bag with the aperture of 1.0-2.0 mm, then putting the mesh bag into a container filled with 1% beta-glucan water solution, and oscillating the mesh bag on a table oscillator for 30 s; taking out the packing bag, airing, and putting the packing bag into a reactor, wherein the filling rate of the reactor is 50%;
(3) the prepared sewage is not added with any organic carbon source. Preparing sewage and activated sludge according to a volume ratio of 1: 3, mixing; a stirring device is additionally arranged and is stirred at the speed of 60 rpm/min; the activated sludge comes from anaerobic tank sludge of sewage treatment plants in Taiyuan city;
(4) the reactor is made of organic glass and is cylindrical, the inner diameter is 5cm, the height is 45cm, and the effective volume is 0.88L, as shown in figure 2; and pumping the mixed water sample from the bottom, allowing the mixed water sample to flow out from the top, continuously running for 48 hours, and controlling the temperature to be 15-17 ℃. Continuously injecting the prepared sewage into the reactor by using a peristaltic pump, wherein the hydraulic retention time is 1.5 h;
(5) then taking effluent every 24 hours, filtering the effluent through a 0.45-micrometer filter membrane, and testing the content of nitrate nitrogen and DOC and the pH value; ensuring enough sewage preparation and maintaining the operation of the reactor;
(6) after 3 days, the nitrogen content of nitrate in the effluent of the reactor can be stably reduced from 40mg/L to below 0.5mg/L, the DOC content is continuously maintained at 0.1-5 mg/L, and the pH value is changed between 6.89-7.21.
Example 2
(1) PHBV in mass ratio: sodium bicarbonate: anhydrous ferric chloride: anhydrous manganese chloride = 100: 10: 0.020: 0.010 to prepare a modified material.
(2) The oscillation time is 15s, and the mixed water sample is continuously injected for 36 h; the rest of the filler treatment, wastewater formulation, reactor specifications, operating conditions, test methods, etc. were the same as in example 1.
(3) After 3 days, the nitrogen content of nitrate in the effluent of the reactor can be stably reduced from 40mg/L to below 2.5mg/L, the DOC content is continuously kept at 2.5-5.5 mg/L, and the pH value is changed between 6.71-7.33.
Example 3
(1) PHBV in mass ratio: sodium bicarbonate: anhydrous ferric chloride: anhydrous manganese chloride = 100: 10: 0.015: 0.070 to modify the material.
(2) The oscillation time is 15s, and the mixed water sample is continuously injected for 36 h; the rest of the filler treatment, wastewater preparation, reactor specifications, operating conditions, and test methods were the same as in example 1.
(3) After 3 days, the nitrogen content of nitrate in the effluent of the reactor can be stably reduced from 40mg/L to below 2.0mg/L, the DOC content is continuously kept at 5.0-7.0 mg/L, and the pH value is changed between 6.95-7.25.
The measured data results of the examples can meet the ideal requirements. Reactor mean NO3 -The N removal rate is more than 95 percent and the highest rate is 98 percent; the DOC concentration does not influence the COD of the effluent; the pH value is in the range suitable for the growth of the denitrifying bacteria; the film forming speed is 1 day faster than that of a PHBV raw material reactor. Therefore, the modified PHBV can be used as a more excellent solid carbon source.
The foregoing description of the examples is provided to enable any person skilled in the art to make and use the present invention. It will be apparent to those skilled in the art that modifications may be made to the described examples of the invention and the general principles of the technology may be applied to other examples. Therefore, the present invention is not limited to the above examples, and modifications and improvements made without departing from the scope of the invention should fall within the protection scope of the invention.
Claims (1)
1. A method of using a denitrifying solid carbon source, comprising the steps of:
(1) filling the modified PHBV material into a mesh bag with the aperture of 1.0-2.0 mm to form a filling bag;
(2) pretreating a filling bag: placing the filling bag into a 1-6% beta-glucan water solution, and oscillating for 15-30 s on a table oscillator; taking out the filling bag, airing and putting the filling bag into a reactor, wherein the filling rate of the reactor is 50%;
(3) mixing the prepared sewage and activated sludge according to the volume ratio of = 1: 3, mixing; stirring at the speed of 60rpm/min, and keeping the sludge uniform to prepare mixed sewage;
(4) and (3) fast film hanging: injecting the mixed sewage prepared in the step (3) from the bottom of the reactor, and allowing the mixed sewage to flow out from the top of the reactor for 36-48 hours at a speed of 2.5 ml/min; continuously feeding water into the reactor by using prepared sewage, keeping the hydraulic retention time at 1.5h, and discharging NO3 -When the N concentration is lower than 5mg/L, the film formation is successful;
the denitrification solid carbon source is a modified PHBV material, and the preparation method comprises the following steps: heating the poly-3-hydroxybutyrate-valerate copolymerized ester powder at 160-175 ℃ to form a molten state, wherein the weight ratio of poly-3-hydroxybutyrate-valerate copolymerized ester is as follows: sodium bicarbonate: anhydrous ferric chloride: anhydrous manganese chloride = 100: 5-10: 0.005-0.020: adding sodium bicarbonate, anhydrous ferric chloride and anhydrous manganese chloride in a mass ratio of 0.001-0.010, stirring at a speed of 60-120 rpm/min for 30-60 s, stopping heating, and naturally cooling to prepare the modified PHBV material.
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| JP2001205288A (en) * | 2000-01-28 | 2001-07-31 | Takeda Chem Ind Ltd | Carrier for water treatment, its production method, and apparatus for water treatment |
| CN104448745A (en) * | 2014-12-23 | 2015-03-25 | 大连工业大学 | Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) foaming material and preparation method thereof |
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