CN108243988B - Pet chew with tooth strengthening function and preparation method thereof - Google Patents
Pet chew with tooth strengthening function and preparation method thereof Download PDFInfo
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- CN108243988B CN108243988B CN201810100061.3A CN201810100061A CN108243988B CN 108243988 B CN108243988 B CN 108243988B CN 201810100061 A CN201810100061 A CN 201810100061A CN 108243988 B CN108243988 B CN 108243988B
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- lactobacillus
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- prebiotics
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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- A01K15/025—Toys specially adapted for animals
- A01K15/026—Chewable toys, e.g. for dental care of pets
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a pet chew with a tooth strengthening function and a preparation method thereof, and belongs to the field of pet tooth strengthening toys. The pet chew has a carrier, a binder, a probiotic, and a prebiotic. The probiotics are all human intestinal probiotic flora and beneficial to human bodies. According to the invention, by utilizing the nature of the pet fond of grinding teeth, and by adding probiotics and prebiotics into the pet chew, the opportunity of long-time grinding and biting of the pet is utilized, not only can the teeth of the pet be cleaned, but also the adhesive is utilized to mix the probiotics and the prebiotics, so that a large amount of the probiotics and the prebiotics are adhered to the oral cavity of the pet, and the prebiotics are added to promote the colonization of the probiotics, so that a dominant probiotic group is formed, the growth of oral pathogenic bacteria and harmful bacteria to the human body is inhibited, and the effects of maintaining the oral health of the pet and.
Description
Technical Field
The invention belongs to the field of pet tooth-strengthening toys, and particularly relates to a pet chew with a tooth-strengthening function and a preparation method thereof.
Background
With the development of economy, pets are more and more in the lives of the people. In the process of feeding pets, particularly dogs/cats, food residues are easily adhered to the teeth of the pets, so that tartar and dental calculus of the pets are increased, oral bacteria grow, and periodontal diseases, tooth fracture, mandibular fracture, oral inflammation and the like of the pets are finally caused. It is currently generally accepted that periodontitis is mainly caused by bacteria such as Porphyromonas gingivalis, Fostainers, Fusobacterium nucleatum, and the like. The microbial balance in the pet oral cavity is broken, so that related oral diseases can be caused, and various oral diseases can aggravate the unbalance of microbial flora, promote the breeding of germs and have great influence on the health of pets. Taking pet dogs as an example, the report indicates that the incidence rate of periodontal diseases of pet dogs aged 2-8 years is 60% -80%, and the incidence rate increases with the age. Once the pet suffers from oral diseases, the treatment modes mainly comprise oral cleaning, ultrasonic tartar and tartar removal, broad-spectrum antibiotic anti-inflammation and the like. It takes a lot of time to clean the oral cavity of the pet; the treatment with antibiotics has a certain curative effect in a short time, but the antibiotics can cause the maladjustment of the original microbial flora in the oral cavity, and finally aggravate the oral cavity problem. In addition, pets, especially dogs like to lick the master, easily cause germs in the oral cavity of the pet to spread to the skin (such as mouth, face, arms and the like) of human bodies, influence the health of the human bodies, and are particularly harmful to infants and old people with weak resistance. In some cases, the pet owner licks the pet with oral diseases to cause adverse reactions. However, there is currently no practical solution to this problem.
The pet chew is a pet toy, and is shaped like a bone, a ball, a circle, a stick, shoes, particles and the like. The most common is a dog chew. Because dogs have the preference of biting bones, the designed shape of the dog chews is also suitable for the characteristics of dogs playing. More importantly, there is also a dog chew that is specifically designed for cleaning the mouth of a dog. Regular chewing of the dog chew and regular brushing of the teeth can slow down the formation of plaque and tartar in the mouth of the dog. Regular brushing is also somewhat effective, as it is not a simple task to brush the teeth in dogs. The feeding dog chew can achieve better results than brushing teeth and can reduce the formation of gingivitis and other grime. The chewing process is also a process of stabilizing teeth and cleaning the mouth. In addition, the gum of the dog is itchy in the growth stage, so that the pain of the dog can be relieved, the articles in the house can be protected from being damaged, and the function of cleaning the oral cavity can be achieved.
The prior pet chews are mainly made of animal skins, such as cow skins or pig skins, and also made of starch, animal protein powder, dietary fiber and the like which are mixed into branched or spherical chews, and also made of rubber by mould pressing. However, the chewing gum only aims at cleaning the teeth of the pet, namely, the rigid friction generated when the pet chews the chewing gum is utilized to achieve the cleaning purpose. Although food residues can be removed to a certain extent and the formation of tartar and dental plaque is reduced, the problems of adjusting the balance of oral flora and reducing the breeding of germs cannot be solved, and the oral health care solution cannot achieve a good effect on the oral health of pets. In addition, probiotics such as bacillus or lactic acid bacteria are added into the existing pet food, but the aim is to adjust the intestinal health of the pet, and the consideration and the design aiming at the breeding of oral disease germs and infecting owners are not provided. And when the pet food is eaten by the pet, the time of the pet food staying in the oral cavity is short, the probiotics can not obtain enough time at all to be activated to further breed and colonize, and the quantity can not reach enough to form the dominant probiotic flora in the oral cavity.
Disclosure of Invention
The invention aims to provide a pet chew with a tooth strengthening function and a preparation method thereof.
The technical scheme adopted by the invention is as follows:
a pet chew with teeth strengthening function comprises a carrier, an adhesive, probiotics and prebiotics.
Preferably, the pet chew has flexibility and extensibility such that the incorporated binders, probiotics and prebiotics are released by the extensibility of the chew itself during chewing by the pet.
Preferably, the carrier includes, but is not limited to, at least one of fabric, rubber, plastic, starch, protein, fiber, and bone meal, and the protein includes, but is not limited to, vegetable protein, and animal skin.
Preferably, the carrier comprises a commercially available pet toy.
Preferably, the adhesive is a vegetable adhesive and/or an animal adhesive, wherein the vegetable adhesive includes but is not limited to at least one of starch, cellulose, tannin, gum arabic and sodium alginate, and the animal adhesive includes but is not limited to at least one of bone glue, fish glue, blood protein glue, casein and shellac.
Preferably, the probiotics are all human intestinal probiotic groups and beneficial to human bodies; the probiotic comprises a bifidobacterium and/or lactobacillus and/or bacillus probiotic.
Preferably, the bifidobacterium species includes, but is not limited to, at least one of bifidobacterium bifidum, bifidobacterium animalis, bifidobacterium breve, bifidobacterium infantis, bifidobacterium adolescentis, bifidobacterium longum.
Preferably, the lactobacillus includes but is not limited to at least one of lactobacillus acidophilus, lactobacillus casei, lactobacillus crispatus, lactobacillus bulgaricus, lactobacillus delbrueckii subspecies lactobacillus, lactobacillus fermentum, lactobacillus gasseri, lactobacillus helveticus, lactobacillus johnsonii, lactobacillus paracasei, lactobacillus plantarum, lactobacillus reuteri, lactobacillus rhamnosus, lactobacillus salivarius.
Preferably, the bacillus includes but is not limited to at least one of bacillus coagulans, bacillus subtilis and bacillus licheniformis.
Preferably, the probiotics is one or more of bifidobacterium, lactobacillus and bacillus which are mixed according to any proportion.
Preferably, the culture medium and culture method of the probiotics are the conventional culture medium and culture method in the field.
Preferably, the probiotics are also added with a proper amount of freeze-drying protective agent. The probiotics is probiotic powder obtained by adding a proper amount of freeze-drying protective agent into the live bacteria mud and freeze-drying the mixture.
Preferably, prebiotics include, but are not limited to, functional oligosaccharides; the functional oligosaccharide includes but is not limited to at least one of isomaltooligosaccharide, fructo-oligosaccharide, galacto-oligosaccharide, xylo-oligosaccharide, lactulose-oligosaccharide, soybean oligosaccharide and inulin.
Preferably, the pet chew comprises the following components in percentage by mass: 0.1 to 3 percent of probiotics, 1 to 20 percent of prebiotics, 0.5 to 10 percent of adhesive, 25 to 45 percent of animal/plant protein powder, 15 to 40 percent of starch and 5 to 15 percent of bone meal.
Preferably, the pet chew comprises the following components in percentage by mass: 0.1 to 1 percent of probiotics, 10 to 20 percent of prebiotics, 0.5 to 10 percent of adhesive, 30 to 40 percent of animal/plant protein powder, 20 to 35 percent of starch and 5 to 15 percent of bone meal.
A preparation method of the pet chew with the tooth strengthening function comprises the steps of weighing all the components of any one of the pet chews, uniformly mixing, extruding/molding and shaping to prepare the pet chew with the tooth strengthening function.
A preparation method of a pet chew with a tooth strengthening function comprises the steps of weighing all components of the pet chew, uniformly mixing probiotics, prebiotics and components except for an adhesive, extruding/molding for shaping, making a carrier, adhering part of the adhesive to the surface of the carrier, and adhering the premixed probiotics, prebiotics and adhesive to the surface of the carrier to prepare the pet chew with the tooth strengthening function.
A preparation method of a pet chew with a tooth strengthening function comprises the steps of weighing all the components of the pet chew, adhering part of an adhesive to the surface of a carrier (such as a commercially available pet toy), and adhering pre-mixed probiotics, prebiotics and the adhesive to the surface of the carrier to prepare the pet chew with the tooth strengthening function.
The pet chew prepared by the materials adopts a technical method commonly used in the field if no special description is provided. The production process according to the present invention can be further scaled up by incorporating basic knowledge in the art.
The invention has the beneficial effects that:
the pet chew of the invention is a pet toy with tooth strengthening function, has certain flexibility and ductility, is not easy to break in the chewing process of a pet, not only fully meets the natural tooth grinding preference of the pet, but also can release probiotics, prebiotics and adhesive through the ductility of the chew, not only can clean the teeth of the pet, simultaneously utilizes the adhesive to mix the probiotics and the prebiotics to enable a large amount of probiotics and the prebiotics to be adhered to the teeth and the oral cavity of the pet, and promotes the permanent planting of the probiotics by the prebiotics to form dominant probiotic groups, inhibits the growth of the oral pathogenic bacteria, achieves the effects of well maintaining the teeth and the oral cavity of the pet and eliminating the peculiar smell of the oral cavity malodor and the like.
The carrier, the adhesive and the prebiotics contained in the pet chew play a role of embedding, and the germination time of the probiotics can be controlled by adjusting the rehydration time of the adhesive and the prebiotics, so that the function of multiple use is achieved.
The preferable prebiotics in the invention are isomaltooligosaccharide, fructo-oligosaccharide, galacto-oligosaccharide, xylo-oligosaccharide, lactulose oligosaccharide, soybean oligosaccharide, inulin and the like. The prebiotics achieve the purpose of optimizing flora balance by promoting the beneficial bacteria to propagate into dominant flora. The prebiotics can also combine with pathogenic bacteria cells to weaken the adhesion of pathogenic bacteria to mucosa, thereby preventing infection.
The selected probiotics are probiotics which are derived from human intestinal probiotic flora and are beneficial to human bodies. The probiotics are fixedly planted in the teeth and the oral cavity of the pet by the chewing of the chewing gum of the pet, so that the harmful germs to the human body are inhibited, the teeth and the oral cavity of the pet can be maintained to be healthy, and the risk of the pet, particularly a dog, to the human body after licking the owner is reduced. The preferred probiotics are bifidobacterium, lactobacillus and bacillus, such as lactobacillus reuteri, lactobacillus rhamnosus, lactobacillus salivarius, bacillus coagulans and the like, and the probiotics are implanted into the teeth and oral cavity of the pet to inhibit harmful microorganisms in the teeth and oral cavity of the pet so as to maintain the health of the teeth and oral cavity of the pet. Wherein, the lactobacillus salivarius can survive in the low pH environment generated by the streptococcus mutans and can specifically inhibit the streptococcus mutans. And the lactobacillus reuteri can secrete roxithromycin and Roy periodic protein, both of which have broad-spectrum antibacterial action and have important effects on preventing decayed teeth and maintaining oral health. Bacillus coagulans has been found to be important for maintaining oral health, to be effective in inhibiting the growth of Streptococcus mutans, to help enhance immune system function against pathogenic organisms, and to reduce inflammatory cytokines.
The pet chew of the invention has rich forms, can be used as a pet toy, and can ensure the health of pets.
The pet chew of the invention can be added with a little protein powder and bone powder to provide flavor, and can utilize the function of probiotics without providing nutrition and energy to the pet, thereby achieving the purpose of maintaining the health of the teeth and oral cavity of the pet while having good chewing and grinding functions to the teeth of the pet.
Drawings
FIG. 1 is a diagram of a pet chew product of the present invention;
FIG. 2 is a diagram of the pet chew product of the present invention;
FIG. 3 is a diagram of the pet chew product of the present invention;
FIG. 4 is a diagram of a pet chew product of the present invention;
FIG. 5 shows the growth of the probiotic combination in coculture with Streptococcus mutans;
FIG. 6 shows the growth of the probiotic combination co-cultured with Porphyromonas gingivalis;
FIG. 7 shows the growth of the probiotic combination co-cultured with F.nucleatum;
FIG. 8 shows the growth of the probiotic combination co-cultured with Fostainer;
FIG. 9 shows the growth of pathogenic bacteria in the oral cavity of dogs before and after the experiment;
fig. 10 shows the growth of probiotics in the oral cavity of dogs after the experiment.
Detailed Description
In the following examples, unless otherwise specified, techniques commonly used in the art are employed. The production process according to the present invention can be further scaled up by incorporating basic knowledge in the art.
Example 1
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
0.1% of probiotics, 1% of isomaltooligosaccharide, 10% of cellulose, 20% of fish gelatin, 23.9% of fibrin glue, 40% of starch and 5% of bone meal.
Wherein the probiotic bacteria comprise Bifidobacterium bifidum, Bifidobacterium breve, Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus delbrueckii subspecies lactis, Bacillus coagulans, Bacillus subtilis, and Bacillus licheniformis.
Probiotic bacteria of the genus bifidobacterium (including bifidobacterium bifidum and bifidobacterium breve): inoculating Bifidobacterium bifidum and Bifidobacterium breve into BS liquid culture medium at an inoculum size of 5%, culturing at 37 deg.C for 48 hr, collecting the thallus to obtain viable count of 1.0-5.0 × 108CFU/g of Bifidobacterium bifidum and Bifidobacterium breve.
Probiotic bacteria of the genus Lactobacillus (including Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus,Lactobacillus crispatus, lactobacillus delbrueckii subsp lactis): inoculating Lactobacillus acidophilus, Lactobacillus crispatus and Lactobacillus delbrueckii subspecies lactis into MRS liquid culture medium at an inoculum size of 5%, culturing at 37 deg.C for 48 hr, collecting thallus to obtain viable bacteria count of 1.0-5.0 × 108CFU/g of Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus delbrueckii subspecies lactis.
Probiotic bacteria of the genus bacillus (including bacillus coagulans, bacillus subtilis, bacillus licheniformis): inoculating Bacillus coagulans, Bacillus subtilis and Bacillus licheniformis into LB culture medium at an inoculum size of 5%, culturing at 37 deg.C for 48 hr, collecting the thallus to obtain viable bacteria with viable count of 1.0-5.0 × 108CFU/g of Bacillus coagulans, Bacillus subtilis and Bacillus licheniformis.
The method comprises the steps of mixing bifidobacterium bifidum, bifidobacterium breve, lactobacillus acidophilus, lactobacillus crispatus, lactobacillus delbrueckii subspecies lactis, bacillus coagulans, bacillus subtilis and bacillus licheniformis according to the equal mass ratio (1:1:1:1:1:1: 1) to obtain probiotic bacterial mud, and mixing the probiotic bacterial mud and a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-drying to obtain probiotic bacterial powder.
The preparation method comprises the following steps: weighing the components of the pet chew according to the proportion, uniformly mixing, and carrying out die pressing and shaping to prepare the pet chew with the tooth strengthening function. The product is shown in fig. 1, which comprises an axonometric view (upper) and a sectional view (lower) of the product, wherein the symbol 1 is the product, 11 is the carrier, and 12 is the tooth-strengthening member. As can be seen from the figure, the prebiotics and probiotics are homogeneously dispersed in the carrier.
Example 2
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
0.3% of probiotics, 8% of fructo-oligosaccharide, 10% of galacto-oligosaccharide, 0.5% of bone glue, 20% of tannin, 18.2% of acacia gum, 37% of starch and 6% of bone meal.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108Youth of CFU/gThe freeze-drying protective agent is prepared by mixing bifidobacterium, bifidobacterium longum, lactobacillus gasseri, lactobacillus helveticus, lactobacillus johnsonii, bacillus coagulans and bacillus subtilis according to equal mass, mixing with a freeze-drying protective agent according to the mass ratio of 1:1, and freeze-drying.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: fully and uniformly mixing starch and bone meal, molding and shaping to prepare a carrier, adhering tannin on the surface of the carrier, and adhering a pre-uniformly mixed mixture of probiotics, prebiotics, bone glue and gum arabic on the surface of the carrier to prepare the tooth-strengthening pet chew. The result is shown in fig. 2, which includes an axonometric view (upper) and a sectional view (lower) of the product, wherein the symbol 1 is the product, 11 is the carrier and 12 is the tooth-strengthening member. It can be seen from the figure that the prebiotics and probiotics are adhered to the surface of the carrier by the action of the adhesive.
Example 3
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
0.5% of probiotics, 3% of xylo-oligosaccharide, 9.5% of sodium alginate, 20% of casein, 25% of shellac, 35% of starch and 7% of bone meal.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108CFU/g of bifidobacterium animalis, bifidobacterium infantis, lactobacillus paracasei, lactobacillus bulgaricus, lactobacillus casei, lactobacillus fermentum, bacillus coagulans and bacillus licheniformis are mixed according to equal mass, and are mixed with a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-dried to obtain the compound.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: weighing the components of the pet chew according to the proportion, uniformly mixing, and carrying out die pressing and shaping to prepare the pet chew with the tooth strengthening function. The product is shown in fig. 3, which includes an axonometric view (top) and a sectional view (bottom) of the product, wherein the symbol 11 is the carrier and 12 is the tooth-strengthening member. As can be seen from the figure, the prebiotics and probiotics are homogeneously dispersed in the carrier.
Example 4
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
0.7% of probiotics, 8% of lactosucrose, 8% of soybean oligosaccharide, 2% of fish gelatin, 20% of cellulose, 23.3% of sodium alginate, 30% of starch and 8% of bone meal.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108CFU/g of bifidobacterium bifidum, bifidobacterium adolescentis, lactobacillus acidophilus, lactobacillus gasseri, lactobacillus paracasei, bacillus subtilis and bacillus licheniformis are mixed according to equal mass, and are mixed with a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-dried to obtain the compound.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: fully and uniformly mixing starch and bone meal, molding and shaping to prepare a carrier, adhering sodium alginate on the surface of the carrier, and adhering a mixture of probiotics, prebiotics, fish gelatin and cellulose which are uniformly mixed in advance on the surface of the carrier to prepare the tooth-strengthening pet chew. The result is shown in fig. 4, comprising an axonometric view (upper) and a sectional view (lower) of the product, in which the symbol 11 is the carrier and 12 is the tooth-strengthening member. As can be seen from the figure, the prebiotics and probiotics are attached to the surface of the carrier by the action of the adhesive.
Example 5
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
0.9% of probiotics, 5% of inulin, 2.5% of gum arabic, 20% of bone glue, 20.6% of fish glue, 22% of starch, 9% of bone meal and 20% of rubber.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108cfu/g of bifidobacterium bifidum, bifidobacterium longum, lactobacillus crispatus, lactobacillus helveticus, lactobacillus bulgaricus, bacillus coagulans, bacillus subtilis and bacillus licheniformis which are mixed according to equal mass and are mixed with a freeze-drying protective agentMixing according to the mass ratio of 1:1 and freeze-drying to obtain the product.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: the method comprises the steps of uniformly mixing starch and bone meal, molding the mixture with rubber to form a carrier, adhering fish glue to the surface of the carrier, and adhering a pre-uniformly mixed mixture of probiotics, prebiotics, gum arabic and bone glue to the surface of the carrier to prepare the pet chew with the tooth strengthening function.
Example 6
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
2.4% of probiotics, 7% of isomaltooligosaccharide, 7% of fructo-oligosaccharide, 7% of fibrin glue, 15% of cellulose, 15.6% of tannin, 32% of starch and 14% of bone meal.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108cfu/g of bifidobacterium bifidum, bifidobacterium animalis, lactobacillus delbrueckii subsp lactis, lactobacillus johnsonii, lactobacillus casei, bacillus coagulans and bacillus subtilis, and the mixture is mixed with a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-dried to obtain the composition.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: weighing the components of the pet chew according to the proportion, uniformly mixing, and carrying out die pressing and shaping to prepare the pet chew with the tooth strengthening function.
Example 7
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
1.7% of probiotics, 8% of galacto-oligosaccharides, 4% of tannin, 20% of fibrin glue, 20.3% of casein, 35% of starch and 11% of bone meal.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108cfu/g Bifidobacterium bifidum, Bifidobacterium infantis, GrignardThe freeze-drying protective agent is prepared by mixing lactobacillus, lactobacillus fermentum, lactobacillus salivarius, bacillus coagulans and bacillus licheniformis in equal mass, mixing with a freeze-drying protective agent in a mass ratio of 1:1, and freeze-drying.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: fully and uniformly mixing starch and bone meal, molding and shaping to prepare a carrier, adhering casein to the surface of the carrier, and adhering a pre-uniformly mixed mixture of probiotics, prebiotics, tannin and fibrin glue to the surface of the carrier to prepare the tooth-strengthening pet chew.
Example 8
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
2% of probiotics, 5% of xylo-oligosaccharide, 5% of lactulose-oligosaccharide, 5% of casein, 20% of gum arabic, 23% of sodium alginate, 28% of starch and 12% of bone meal.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108cfu/g of bifidobacterium bifidum, bifidobacterium breve, bifidobacterium adolescentis, lactobacillus gasseri, lactobacillus bulgaricus, lactobacillus reuteri, bacillus subtilis and bacillus licheniformis are mixed according to equal mass, and are mixed with a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-dried to obtain the composition.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: weighing the components of the pet chew according to the proportion, uniformly mixing, and carrying out die pressing and shaping to prepare the pet chew with the tooth strengthening function.
Example 9
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
2.2% of probiotics, 6% of soybean oligosaccharide, 6% of inulin, 6% of lac, 17% of cellulose, 20% of acacia gum, 30% of starch and 13% of bone meal.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108cfu/g of bifidobacterium breve, bifidobacterium longum, lactobacillus helveticus, lactobacillus casei, lactobacillus rhamnosus, bacillus coagulans and bacillus subtilis are mixed according to equal mass, and the mixture is mixed with a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-dried to obtain the composition.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: fully and uniformly mixing starch and bone meal, molding and shaping to prepare a carrier, adhering gum arabic to the surface of the carrier, and adhering a pre-uniformly mixed mixture of probiotics, prebiotics, lac and cellulose to the surface of the carrier to prepare the tooth-strengthening pet chew.
Example 10
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
1.4% of probiotics, 7% of fructo-oligosaccharide, 3% of cellulose, 15% of bone glue, 20% of lac, 24% of starch, 10% of bone meal and 20% of woven fabric.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108cfu/g of bifidobacterium breve, bifidobacterium animalis, lactobacillus johnsonii, lactobacillus fermentum, lactobacillus plantarum, bacillus coagulans and bacillus licheniformis which are mixed according to the equal mass, and the mixture is mixed with a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-dried to obtain the freeze-dried bifidobacterium.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: fully and uniformly mixing starch and bone meal, molding and shaping with woven fabric to prepare a carrier, adhering lac on the surface of the carrier, and adhering a pre-uniformly mixed mixture of probiotics, prebiotics, cellulose and bone glue on the surface of the carrier to prepare the pet chew with the tooth strengthening function.
Example 11
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
2.6% of probiotics, 7% of galacto-oligosaccharide, 8% of xylo-oligosaccharide, 8% of tannin, 5% of bone glue, 10% of blood protein glue, 10% of casein, 34% of starch and 15% of bone meal.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108cfu/g of bifidobacterium breve, bifidobacterium infantis, lactobacillus acidophilus, lactobacillus helveticus, lactobacillus fermentum, lactobacillus salivarius, bacillus coagulans and the like, and the mixture is mixed with a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-dried to obtain the composition.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: weighing the components of the pet chew according to the proportion, uniformly mixing, and carrying out die pressing and shaping to prepare the pet chew with the tooth strengthening function.
Example 12
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
3% of probiotics, 5% of lactosucrose, 5% of soybean oligosaccharide, 10% of inulin, 10% of fish gelatin, 10% of cellulose, 10% of gum arabic, 17% of sodium alginate, 15% of starch and 15% of bone meal.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108cfu/g of bifidobacterium adolescentis, bifidobacterium animalis, lactobacillus crispatus, lactobacillus johnsonii, lactobacillus reuteri and bacillus subtilis which are mixed according to the equal mass, and are mixed with a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-dried to obtain the composition.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: fully and uniformly mixing starch and bone meal, molding and shaping to prepare a carrier, adhering sodium alginate on the surface of the carrier, and adhering a pre-uniformly mixed mixture of probiotics, prebiotics, fish gelatin, cellulose and gum arabic on the surface of the carrier to prepare the tooth-strengthening pet chew.
Example 13
A pet chew with teeth strengthening function comprises carrier, adhesive, probiotic bacteria and prebiotics. The pet chew is composed of the following components in percentage by mass:
2.8% of probiotics, 6% of lactosucrose, 7% of fibrin glue, 10% of tannin, 18.2% of sodium alginate, 27% of starch, 9% of bone meal and 20% of commercial pet toys.
The number of the activated viable bacteria of the probiotic powder is 1.0-5.0 multiplied by 108cfu/g of bifidobacterium longum, bifidobacterium animalis, bifidobacterium infantis, lactobacillus delbrueckii subsp lactis, lactobacillus bulgaricus, lactobacillus rhamnosus and bacillus licheniformis which are mixed according to the same mass ratio, and are mixed with a freeze-drying protective agent according to the mass ratio of 1:1 and freeze-dried to obtain the composition.
The probiotic culture method was the same as in example 1.
The preparation method comprises the following steps: the method comprises the steps of uniformly mixing starch and bone meal, then carrying out die pressing and shaping on the mixture and a commercially available pet toy to prepare a carrier, then adhering sodium alginate to the surface of the carrier, and then adhering a pre-uniformly mixed mixture of probiotics, fibrin glue and tannin to the surface of the carrier to prepare the pet chew with the tooth strengthening function.
The present invention is not limited to the above-described embodiments, which are merely preferred examples of the present invention, but are not intended to limit the present invention, and it will be apparent to those skilled in the art that various modifications and variations can be made without departing from the basic spirit of the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention shall be included in the scope of the present invention.
In order to test the recovery effect of the pet chew on the oral diseases, the curative effect of the pet chew on the periodontal diseases is verified by a co-culture experiment of strains and a recovery experiment of the canine periodontal diseases.
1. Study on bacteriostatic properties
Diluting the culture of pathogenic bacteria of periodontal disease such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Fostainer to 10%6CFU/mLThen 0.1mL of beef extract-coated peptone medium plate was taken, and an Oxford cup (diameter 8mm) was placed on the plate, 100. mu.L of the probiotic bacteria solution of example 1 was added thereto, and after culturing at 37 ℃ for 24 hours, the size of the zone of inhibition was measured, and a blank control was made with sterile physiological saline. The results of the experiment are shown in table 1.
TABLE 1 diameter of zone of inhibition (unit: mm)
Note: -no bacteriostatic action
Table 1 the experimental results show that: the probiotic combination has good inhibition effect on four pathogenic bacteria, namely streptococcus mutans, porphyromonas gingivalis, fusobacterium nucleatum and Fostainer.
2. Co-culture experiment of strains
The probiotic combination of example 1 was co-cultured with Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Fostanemia furiosaensis, respectively, in liquid medium at 37 deg.C, and samples were taken at different culture times, respectively, and the inhibition of the probiotic against pathogenic bacteria was determined by plate counting.
The probiotic combination was co-cultured with Streptococcus mutans and growth was as shown in FIG. 5.
In FIG. 5, after incubation of the probiotic combination with Streptococcus mutans for 36h, no Streptococcus mutans is present, indicating that the probiotic combination has an inhibitory effect on Streptococcus mutans.
The probiotic combination was co-cultured with Porphyromonas gingivalis and the growth profile is shown in FIG. 6.
In fig. 6, after the probiotic combination and the porphyromonas gingivalis are co-cultured for 16h, no porphyromonas gingivalis exists, which indicates that the probiotic combination has an inhibiting effect on the porphyromonas gingivalis.
The probiotic combination was co-cultured with fusobacterium nucleatum, and the growth profile is shown in fig. 7.
In FIG. 7, after the probiotic combination and the F.nucleatum are co-cultured for 16h, no F.nucleatum exists, indicating that the probiotic combination has an inhibitory effect on the F.nucleatum.
The probiotic combination was co-cultured with Fostainer and the growth profile is shown in FIG. 8.
In FIG. 8, the probiotic combination had no Fostainer present after 20h of co-culture with Fostainer, indicating that the probiotic combination had inhibitory effect on Fostainer.
3. Dog periodontal disease rehabilitation experiment
The experiment was performed using 8 groups of 3 dogs, each 3 dogs having periodontal disease and oral malodor, 3 dogs.
Experimental group 1: applying the pet chew of example 1 daily;
experimental group 2: the pet chew of example 2 was applied daily;
experimental group 3: applying the pet chew of example 5 daily;
experimental group 4: applying the pet chew of example 13 daily;
experimental group 5: daily applying a pet chew formulated as in example 5 with the probiotic, prebiotic and binder components removed, i.e. the formulation contains only starch, bone meal and rubber;
experimental group 6: applying the dog chews which are purchased in the market and take pig skin as the raw material every day;
experimental group 7: applying the dog chews which are purchased in the market and take rubber as raw materials every day;
experimental group 8: applying a dog food containing lactobacillus purchased in the market every day;
the chews or dog food of the experimental groups were set to equal volumes. Continuously feeding for 14 days, randomly taking bacteria from two sides of periodontal tissues of oral cavities of eight groups of dogs by using sterilized filter paper sheets with the size of 1cm multiplied by 1cm at the beginning and the end of an experiment, immersing the bacteria into 1.0mL of physiological saline, then calculating the number of bacterial colonies by using a plate counting method after diluting according to 10 times of series, observing the growth condition of the bacteria in the oral cavities between experimental groups, and simultaneously judging whether the stink in the oral cavities of the dogs is improved or not before and after the experiment.
The growth of pathogenic bacteria in the oral cavity of dogs before and after the experiment is shown in figure 9.
The results in FIG. 9 show that: after 14-day feeding experiments, the number of pathogenic bacteria in the oral cavities of the dogs in experimental groups 1, 2, 3 and 4 is obviously reduced, while the number of pathogenic bacteria in the oral cavities of the dogs in experimental group 5, experimental group 6 and experimental group 7 is not obviously reduced, and the number of pathogenic bacteria in the oral cavity of the experimental group 8 is slightly increased. The experimental result shows that the pet chew has obvious inhibiting effect on pathogenic bacteria of periodontal disease.
Results of malodor identification in the oral cavity of dogs before and after the experiment are shown in table 2.
TABLE 2 determination of dog oral malodor JI before and after experiment
Note: + indicates a malodorous odour and-indicates no malodorous odour
Table 2 the experimental results show that: after the feeding experiment for 14 days, the malodorous smells in the oral cavities of the dogs of the experimental groups 1, 2, 3 and 4 disappeared, while the malodorous smells in the oral cavities of the dogs of the experimental groups 5, 6, 7 and 8 remained. The experimental result shows that the pet chew has obvious improvement effect on the oral malodor of the dog.
4. Bite time and probiotic colonization experiment
In order to study the biting time of the pet chew and the situation that probiotics are fixedly planted on pet teeth, the design experiment is as follows:
the experiment was performed using 8 groups of 3 periodontal healthy dogs aged 3 years, each group containing 3 dogs.
Experimental group 1: applying the pet chew of the embodiment 1, wherein the biting time is about 60 seconds;
experimental group 2: applying the pet chew of the embodiment 1, wherein the biting time is about 120 seconds;
experimental group 3: applying the pet chew of the embodiment 1, wherein the biting time is about 180 seconds;
experimental group 4: applying the pet chew of the embodiment 1, wherein the biting time is about 240 seconds;
experimental group 5: applying the pet chew which is prepared by the formula of the example 1 and is removed with the components of the probiotics, the prebiotics and the adhesive, namely, the formula only contains starch and bone meal, and the biting time is about 180 seconds;
experimental group 6: applying the dog chews which are purchased in the market and take the pigskin as the raw material, wherein the biting time is about 180 seconds;
experimental group 7: applying the dog chews which are purchased in the market and take rubber as raw materials, wherein the biting time is about 180 seconds;
experimental group 8: applying a dog food containing lactobacillus purchased in the market;
the chews or dog food of the experimental groups were set to equal volumes. Feeding continuously for 14 days, randomly taking bacteria from two sides of periodontal tissues of oral cavities of eight groups of dogs by using sterilized filter paper sheets with the size of 1cm multiplied by 1cm at the end of an experiment, soaking the bacteria into 1.0mL of physiological saline, calculating the colony number by using a plate counting method after serial dilution according to 10 times, and observing the growth condition of probiotics in the oral cavities between experimental groups.
The growth of the probiotic in the oral cavity of the dog after the experiment was completed is shown in figure 10.
The results in FIG. 10 show that: after the 14-day feeding experiment, the number of probiotics was slightly less than in experimental groups 2, 3 and 4, although experimental group 1 was due to the relatively short bite time, and overall there was a large amount of probiotics present in the oral cavity of experimental groups 1, 2, 3 and 4 dogs. The groups 5, 6 and 7 contained no probiotic per se, so no probiotic was detected. The dog food of experimental group 8 was pet food, and as with most pet foods, the dogs consumed the food in a very short time, and the probiotics did not effectively adhere to the teeth, had insufficient time to be activated for further reproduction and colonization, and only a trace amount of probiotics was detected in the oral cavity.
Claims (4)
1. The utility model provides a pet chew that has tooth function which characterized in that: the pet chew comprises, by mass, 45% -67% of a carrier, 0.5% -10% of an adhesive, 0.1% -3% of probiotics and 1% -20% of prebiotics;
the probiotics, prebiotics and adhesive are adhered to the surface of the carrier;
the carrier is made of at least one of woven fabric, rubber, plastic, starch, protein, fiber and bone meal;
the probiotic bacteria comprise bifidobacteria and/or lactobacilli and/or bacillus probiotics, and the bifidobacteria comprise at least one of bifidobacterium bifidum, bifidobacterium animalis, bifidobacterium breve, bifidobacterium infantis, bifidobacterium adolescentis and bifidobacterium longum; the lactobacillus comprises at least one of lactobacillus acidophilus, lactobacillus casei, lactobacillus crispatus, lactobacillus bulgaricus, lactobacillus delbrueckii subspecies, lactobacillus fermentum, lactobacillus gasseri, lactobacillus helveticus, lactobacillus johnsonii, lactobacillus paracasei, lactobacillus plantarum, lactobacillus reuteri, lactobacillus rhamnosus and lactobacillus salivarius; the bacillus comprises at least one of bacillus coagulans, bacillus subtilis and bacillus licheniformis;
the adhesive is a plant adhesive and/or an animal adhesive, and the plant adhesive comprises at least one of starch, cellulose, tannin, gum arabic and sodium alginate; the animal adhesive comprises at least one of bone glue, fish glue, blood protein glue, casein and lac;
the adhesive is used for adhering the probiotics and the prebiotics to the teeth and the oral cavity of the pet;
the prebiotics comprise functional oligosaccharides, and the functional oligosaccharides comprise at least one of isomaltooligosaccharides, fructooligosaccharides, galactooligosaccharides, xylooligosaccharides, lactulose oligosaccharides, soy oligosaccharides and inulin.
2. The pet chew of claim 1, wherein the carrier further comprises a commercially available pet toy.
3. A preparation method of pet chew with tooth strengthening function is characterized in that: weighing the components of the pet chew of any one of claims 1-2, mixing the probiotic bacteria, prebiotics and components other than the adhesive uniformly, extruding or molding to form a carrier, adhering a portion of the adhesive to the surface of the carrier, and adhering the pre-mixed probiotic bacteria, prebiotics and adhesive to the surface of the carrier to produce a pet chew with teeth strengthening function.
4. A preparation method of pet chew with tooth strengthening function is characterized in that: a pet chew having teeth strengthening properties is prepared by weighing the components of the pet chew of any one of claims 1-2, adhering a portion of the adhesive to the surface of the carrier, and then adhering the pre-mixed probiotic bacteria, prebiotics, and adhesive to the surface of the carrier.
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| CN201810100061.3A CN108243988B (en) | 2018-02-01 | 2018-02-01 | Pet chew with tooth strengthening function and preparation method thereof |
| EP18204248.1A EP3479701B1 (en) | 2017-11-03 | 2018-11-05 | Method of preparation of a pet chew toy with oral care properties |
| ES18204248T ES2818999T3 (en) | 2017-11-03 | 2018-11-05 | Chew toy for pets with oral care properties and toy preparation method |
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| CN108243988B true CN108243988B (en) | 2021-06-29 |
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| CN109287870A (en) * | 2018-09-06 | 2019-02-01 | 温州锦恒宠物用品有限公司 | Pet chew and preparation method thereof |
| CN109172615A (en) * | 2018-09-07 | 2019-01-11 | 北京金锋实验室科技有限公司 | For treating the probiotics of self-closing disease patient |
| CN110195029B (en) * | 2019-04-18 | 2023-05-30 | 郑州和合生物工程技术有限公司 | Bacillus coagulans for efficiently antagonizing streptococcus mutans, preparation method of bacterial powder, chewing gum containing bacterial powder and application |
| GB201908109D0 (en) * | 2019-06-06 | 2019-07-24 | Mars Inc | Use of oral chew |
| WO2022050794A1 (en) * | 2020-09-07 | 2022-03-10 | 일동제약(주) | Composition for preventing or treating periodontal diseases, comprising bacillus velezensis strain, culture medium thereof, or culture supernatant thereof as active ingredient |
| CA3191643A1 (en) * | 2020-09-07 | 2022-03-10 | Byeonghun Lee | Novel bacillus velezensis strains and use thereof |
| CN114208966A (en) * | 2022-01-18 | 2022-03-22 | 金卫 | Pet probiotic health-care food |
| CN114467791B (en) * | 2022-01-27 | 2022-10-18 | 杭州易宠科技有限公司 | Pet tooth cleaning bone and preparation method thereof |
| CN116138462A (en) * | 2023-03-29 | 2023-05-23 | 杭州知零生物科技有限公司 | A metagen composition for enhancing resistance and its preparation method |
| CN116491590A (en) * | 2023-06-01 | 2023-07-28 | 上海福智宠物食品有限公司 | Puffed food for improving tooth health of pets |
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| CN101233891A (en) * | 2007-02-01 | 2008-08-06 | 大连森佰澳科技有限公司 | Health care chewing gum containing probiotics signal molecule |
| US20090235872A1 (en) * | 2008-03-19 | 2009-09-24 | Nevenka Filipi | Increased occupancy time pet chews |
| WO2014004994A1 (en) * | 2012-06-29 | 2014-01-03 | The Iams Company | Pet food with auditory stimulus |
| WO2014098823A1 (en) * | 2012-12-19 | 2014-06-26 | Hill's Pet Nutrition, Inc. | Animal food composition and method |
| CN103652447B (en) * | 2013-12-05 | 2015-08-05 | 青岛天地荟食品有限公司 | A kind of ox-hide stings glue pet dog food and preparation technology thereof |
| CN104382949A (en) * | 2014-11-07 | 2015-03-04 | 南昌大学 | Preparation method of probiotic chewing gum |
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