CN108239611A - One plant of brevibacterium and its method for heavy metals in farmland pollution in-situ immobilization - Google Patents
One plant of brevibacterium and its method for heavy metals in farmland pollution in-situ immobilization Download PDFInfo
- Publication number
- CN108239611A CN108239611A CN201611216477.9A CN201611216477A CN108239611A CN 108239611 A CN108239611 A CN 108239611A CN 201611216477 A CN201611216477 A CN 201611216477A CN 108239611 A CN108239611 A CN 108239611A
- Authority
- CN
- China
- Prior art keywords
- brevibacterium
- parts
- weight
- farmland
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/13—Brevibacterium
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The present invention provides one plant of brevibacterium and its method for heavy metals in farmland pollution in-situ immobilization, and the Classification And Nomenclature of the brevibacterium is：Brevibacterium (Brevibacterium sp) GRINM L2, are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, preservation date：On September 29th, 2016, deposit number：CGMCC No.13064.Insoluble phosphate efficiently can be converted into soluble phosphate by the bacterium in the presence of high concentration heavy metal ion, the reparation available for heavy metal pollution farmland.
Description
Technical field
The invention belongs to microbe subject fields, are related to one plant of brevibacterium, which can be in high concentration heavy metal
It survives in the presence of ion, insoluble phosphate is converted into soluble phosphate and the brevibacterium pollutes for heavy metals in farmland
The method of in-situ immobilization.
Background technology
China's heavy metals in farmland is seriously polluted, and existing administration way is mainly based on chemical passivation, and quite a few is changed
It is fungicide to learn passivator (such as lime, vulcanized sodium etc.), and soil is easily killed during heavy metals in farmland pollution control is carried out
The problems such as indigenous microorganism group in earth causes soil hardening, and fertility declines.On the other hand, there is 74% arable land soil in China
Earth lacks phosphorus, since the phosphate fertilizer of application is easily cured, cause 95% and its above phosphorus by be difficult to be utilized it is invalid in the form of exist,
Farmland phosphate fertilizer often excessively adds, and has further resulted in the hardened of soil.It therefore, will if the dissolving P capacity of microorganism can be utilized
In by the farmland of heavy metal pollution, the unemployed phosphorus formed due to excessive phosphate fertilizer adds is converted into titanium pigment, on the one hand
Titanium pigment can form insoluble phosphate with heavy metal, on the other hand in-situ solidifying heavy metal can be provided for plant growth
It is sustained and effective phosphorus.
Invention content
First purpose of the present invention is to provide one plant of brevibacterium brevibacterium strain, which can be dense in high heavy metal ion
In the presence of degree, efficiently the phosphorus in calcium phosphate is released, forms PO4 3-, and PO4 3-Can with heavy metal ion for example copper, lead,
Zinc etc. forms insoluble metal phosphate.
Second object of the present invention is to provide a kind of culture medium can be enriched with, detach, cultivating the bacterium.
Third object of the present invention is to provide a kind of method polluted using the bacterium in situ remediation heavy metals in farmland.
The purpose of the present invention is what is be achieved through the following technical solutions：
The present invention provides one plant of brevibacterium, and the Classification And Nomenclature of the bacterium is brevibacterium (Brevibacterium sp.) GRINM
L2, depositary institution are：China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are：Chaoyang District, Beijing City
The institute 3 of North Star West Road 1, Institute of Microorganism, Academia Sinica, preservation date：On September 29th, 2016, deposit number：CGMCC
No.13064。
The colony characteristics of bacterial strain are as described above：31 DEG C of growths are very fast on solid medium, and colony diameter is after 3 days
3mm, phosphorus decomposing loop diameter are 5mm, and bacterium colony is in regular circle shapes, and color is blue, and quality is fine and close, and transparent circle is circle, is close to bacterium
It is born length.
Bacterial strain as described above efficiently can release the phosphorus in calcium phosphate in the presence of high concentration of heavy metal ion,
Form PO4 3-, and PO4 3-Insoluble metal phosphate can be formed with heavy metal ion such as copper, lead, zinc etc..
For being enriched with, being separately cultured the culture medium of above-mentioned brevibacterium, including：The formula of the culture medium is：10 parts by weight Portugals
Grape sugar, 5 parts by weight of phosphoric acid calcium, 0.3 parts by weight epsom salt, 0.5 parts sulfuric acid ammonium, 0.3 parts by wt NaCl, 0.3 weight
Measure part potassium chloride, 0.002 parts sulfuric acid manganese, 0.03 parts by weight ferrous sulfate heptahydrate, 1 parts by weight yeast extract, 1000 weights
Measure part distilled water, pH7.0~7.5
Above-mentioned culture medium 121 DEG C of sterilizing 30min in high-pressure sterilizing pot are spare.
The enrichment of brevibacterium as described above, isolated culture method, above-mentioned brevibacterium strain is inoculated with into training as described above
It supports in base, under 30-33 DEG C of cultivation temperature, 100rpm shaking table cultures to bacteria concentration are 108A/mL.
The present invention also provides a kind of methods that heavy metals in farmland pollution in-situ immobilization is carried out using brevibacterium as described above, will
The strain of the brevibacterium after carrying out enrichment culture using method as described above, is seeded to heavy metal pollution farmland, inoculum concentration
For every m3Volume 2-4L, preferably 3L.
When repairing effect is bad or has new heavy metal pollution to enter farmland, culture medium as described above can be added.
The preparation method of brevibacterium is as described above：
(1) culture medium
For detaching the solid medium of strain：10g/L glucose, 5g/L calcium phosphate, 0.3g/L epsom salts,
0.5g/L ammonium sulfate, 0.3g/L sodium chloride, 0.3g/L potassium chloride, 0.002g/L manganese sulfates, 0.03g/L ferrous sulfate heptahydrates, 1g/
L yeast extracts, agar 20g, 0.4% bromophenol blue 6mL, 1L water, pH7.0~7.5.
For being enriched with the fluid nutrient medium of strain：10g/L glucose, 5g/L calcium phosphate, 0.3g/L epsom salts,
0.5g/L ammonium sulfate, 0.3g/L sodium chloride, 0.3g/L potassium chloride, 0.002g/L manganese sulfates, 0.03g/L ferrous sulfate heptahydrates, 1g/
L yeast extracts, 1L water, pH7.0~7.5.
Above-mentioned culture medium 121 DEG C of sterilizing 30min in high-pressure sterilizing pot are spare.
(2) concentration and separation of bacterial strain
The initial soil sample of this brevibacterium separation comes from Henan Province Pb-Zn ore district periphery arable land, the arable land continuous three
Year apply phosphate fertilizer, choose corn, peanut, fresh kidney beans, sweet potato, willow crop rhizosphere soil.At from 10~20cm of earth's surface, completely
The root of plant is dug out, blocky larger soil block is shaked off using root method is trembled, root soil of the complete root together with adhesion is packed into nothing
Bacterium sealed bag kind saves backup in laboratory of taking back interior for 24 hours in refrigerator in 4 DEG C.
Taking root, a little is scraped off by the soil blade of adhesion above, and particle is slightly larger to be pulverized, will be from corn, flower
Life, fresh kidney beans, sweet potato, willow crop root soil collect and be uniformly mixed.The soil of precise 35g be put into 200mL go from
In the conical flask of 250mL in sub- water, and add in a small amount of root shredded.300r/min magnetic agitations crush soil 40min, quiet
Put layering.Supernatant is taken to observe, bacterium amount is less.
20mL supernatants is taken to be added in 200mL aforesaid liquid culture mediums, at 31 DEG C in 250mL conical flasks, 160r/
The shaking table enrichment culture of min three days, the concentration for being concentrated into bacterium solution reaches 108A/mL takes out shaking flask, supernatant is taken to carry out respectively
10-1、10-2、10-3、10-4、10-5、10-6, the dilution of six gradients.Then 20 μ L is taken to be coated on solid medium good in advance
On tablet, each concentration in triplicate, in being cultivated three days in 31 DEG C of constant temperature fixed beds, observes the variation of flat-plate bacterial colony daily.
(3) strain isolation is identified
The brevibacterium of enrichment is coated on above-mentioned culture medium, and chooses single bacterium colony and carries out further plate streaking.Purifies and separates
Single bacterium colony afterwards carries out strain morphologic observation using scanning electron microscope；Using 16S rDNA clone libraries technologies to detach pure bacterial strain into
Row identification, qualification result show that the bacterial strain category brevibacterium (Brevibacterium sp.) belongs to.
The beneficial effects of the present invention are：
The present invention provides one plant of brevibacterium brevibacterium (Brevibacterium sp.) GRINM L2 bacterial strains, which should
Strain efficiently can release the phosphorus in calcium phosphate in the presence of high concentration of heavy metal ion, form PO4 3-, and PO4 3-It can
To form insoluble metal phosphate with heavy metal ion such as copper, lead, zinc etc..
Description of the drawings
Fig. 1 is the stereoscan photograph of brevibacterium provided by the invention (Brevibacterium sp.) GRINM L2.
Fig. 2 is the phosphorus decomposing effect of brevibacterium provided by the invention.
Specific embodiment
The invention will be further described by the following examples.
Embodiment 1
Brevibacterium provided by the present invention has carried out preservation, and the Classification And Nomenclature of the bacterium is brevibacterium (Brevibacterium
Sp.) GRINM L2, depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are：Beijing
The institute 3 of city Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, preservation date：On September 29th, 2016, preservation are compiled
Number：CGMCC No.13064.
The cultural method of brevibacterium of the present invention is as follows：
The culture medium (fluid nutrient medium) of enrichment culture brevibacterium is prepared first：10g/L glucose, 5g/L calcium phosphate,
0.3g/L epsom salts, 0.5g/L ammonium sulfate, 0.3g/L sodium chloride, 0.3g/L potassium chloride, 0.002g/L manganese sulfates, 0.03g/
L ferrous sulfate heptahydrates, 1g/L yeast extracts, 1L water, pH7.3.
Solid medium：10g/L glucose, 5g/L calcium phosphate, 0.3g/L epsom salts, 0.5g/L ammonium sulfate, 0.3g/
L sodium chloride, 0.3g/L potassium chloride, 0.002g/L manganese sulfates, 0.03g/L ferrous sulfate heptahydrates, 1g/L yeast extracts, agar
20g, 0.4% bromophenol blue 6mL, 1L water, pH7.0~7.5.
Above-mentioned culture medium 121 DEG C of sterilizing 30min in high-pressure sterilizing pot are spare.
The soil sample containing brevibacterium that the present invention is obtained, comes from Henan Province Pb-Zn ore district periphery arable land, and the arable land is continuous
3 years apply phosphate fertilizer, choose corn, peanut, fresh kidney beans, sweet potato, willow crop rhizosphere soil.It is complete at from 10~20cm of earth's surface
The whole root for digging out plant shakes off blocky larger soil block using root method is trembled, root soil of the complete root together with adhesion is packed into
Sterile sealed bag kind saves backup in laboratory of taking back interior for 24 hours in refrigerator in 4 DEG C.
Taking root, a little is scraped off by the soil blade of adhesion above, and particle is slightly larger to be pulverized, will be from corn, flower
Life, fresh kidney beans, sweet potato, willow crop root soil collect and be uniformly mixed.The soil of precise 35g be put into 200mL go from
In the conical flask of 250mL in sub- water, and add in a small amount of root shredded.300r/min magnetic agitations crush soil 40min, quiet
Put layering.Supernatant is taken to observe, bacterium amount is less.
Take 20mL supernatants in 200mL aforesaid liquids culture medium and 250mL conical flasks at 31 DEG C, the shaking table of 160r/min is rich
Collection culture three days, the concentration of bacterium solution reaches 108A/mL takes out shaking flask, supernatant is taken to carry out 10 respectively-1、10-2、10-3、10-4、
10-5、10-6, the dilution of six gradients.Then 20 μ l is taken to be coated on solid plate good in advance, each concentration repeats three
It is secondary, in being cultivated three days in 31 DEG C of constant temperature fixed beds, the variation of flat-plate bacterial colony is observed daily, and picking takes single bacterium colony culture.
Strain is analyzed and identified with 16S rDNA clone library technologies.By single bacterium colony 10mL fluid nutrient mediums, 31 DEG C,
160r/min shaking table cultures three days, gained 1mL bacterium solutions centrifuge to obtain bacterium mud, total DNA are extracted, using round pcr with prokaryotes
Universal primer 530f and 1490r expand 16S rDNA segments.PCR product is connect after purification with the T-easy carriers of Promega, is turned
Change bacillus coli DH 5 alpha.The blue colonies of picking determine positive bacterium colony by bacterium colony PCR, and through digestion parting, 4 clones are surveyed
Sequence.Gained sequence relatively shows that the bacterial strain belongs to bacterium for brevibacterium (Brevibacterium sp) through Blast, uses scanning electron microscope
Thalli morphology is observed, thalli morphology is as shown in Figure 1.
By the bacterial strain of identification aforementioned liquids medium culture to bacteria concentration 108A/mL, take within continuous 5 days 5mL solution in from
In heart pipe, at 25 DEG C, 11000r/min centrifuges 5min.The supernatant of 100 μ L is taken respectively according to the measuring process of phosphorus standard curve
Absorbance is measured, the size of titanium pigment concentration is calculated finally by function.
It is 0.187nm, 0.190nm, 0.210nm, 0.241nm, 0.254nm by measurement five days light absorption values of ining succession, leads to
A concentration of 78.11mg/L, 79.22mg/L, 86.62mg/L, 98.08mg/L, 102.89mg/L that function calculates its Leaching Properties of Soluble Phosphorus are crossed,
It can be seen that the strain has the ability of phosphorus in slow mechanism dissolved calcium phosphate, as shown in Figure 2.
By bacterium aforementioned liquids culture medium 200mL shaking flasks culture to bacteria concentration 108A/mL, another setting only add in 200mL
Culture medium is not added with the blank control shaking flask of bacterium, and 30mg PbCl are added in above-mentioned two shaking flask2, 20mg ZnCl2, 10mg
CuCl2, shaking table continues culture 20 days, finds plus bacterium shaking flask Pb concentration reduces by 99%, Zn concentration and reduces by 95%, Cu concentration and reduces
90%, show that the bacterium can release the phosphorus in calcium phosphate under high heavy metal concentration, form PO4 3-, and further with a huge sum of money
Category forms insoluble phosphate.
Bacterium fluid nutrient medium is expanded culture to be inoculated with into farmland, inoculum concentration is per m3Volume 3L, calculates farmland after 1 year
Middle heavy metals immobilization rate is more than 95%, and the effect that the insoluble phosphorus in farmland is converted into titanium pigment is suitable with common slow-release phosphate fertilizer.
From above-described embodiment as can be seen that fixed phosphorus can be converted into solubility by brevibacterium provided by the invention
Phosphorus, one side titanium pigment can form insoluble phosphate with heavy metal, on the other hand in-situ solidifying heavy metal can be plant
Growth, which provides, to be sustained and effective phosphorus, is reduced the dosage of common phosphate fertilizer, is reduced the situation of soil hardening.
Claims (6)
1. one plant of brevibacterium, which is characterized in that the Classification And Nomenclature of the bacterium is brevibacterium (Brevibacterium sp) GRINM
L2, depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are：Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1, Institute of Microorganism, Academia Sinica, preservation date：On September 29th, 2016, deposit number：CGMCC
No.13064。
2. for being enriched with, being separately cultured the culture medium of brevibacterium as described in claim 1, which is characterized in that the culture medium is matched
Fang Wei：10 parts by weight glucose, 5 parts by weight of phosphoric acid calcium, 0.3 parts by weight epsom salt, 0.5 parts sulfuric acid ammonium, 0.3 weight
Part sodium chloride, 0.3 parts by weight potassium chloride, 0.002 parts sulfuric acid manganese, 0.03 parts by weight ferrous sulfate heptahydrate, 1 parts by weight yeast
Extract, 1000 parts by weight distilled water, pH7.0~7.5.
3. for the method for brevibacterium described in enrichment culture claim 1, which is characterized in that by quarter butt as described in claim 1
Bacterium is inoculated in culture medium as claimed in claim 2, and under 30-33 DEG C of cultivation temperature, 100rpm shaking table cultures are dense to bacterium
Spend is 108A/mL.
A kind of 4. method that heavy metals in farmland pollution in-situ immobilization is carried out using brevibacterium described in claim 1, which is characterized in that
By the strain of brevibacterium described in claim 1, after carrying out enrichment culture using method as claimed in claim 3, it is inoculated with bacterium solution
To heavy metal pollution farmland.
5. the method as described in power requires 4, which is characterized in that the inoculum concentration for being inoculated with farmland is per m3Volume 2-4L.
6. the method as described in power requires 4 or 5, which is characterized in that the inoculum concentration for being inoculated with farmland is per m3Volume 3L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611216477.9A CN108239611B (en) | 2016-12-26 | 2016-12-26 | Brevibacterium strain and method for in-situ remediation of heavy metal pollution of farmland by using same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611216477.9A CN108239611B (en) | 2016-12-26 | 2016-12-26 | Brevibacterium strain and method for in-situ remediation of heavy metal pollution of farmland by using same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108239611A true CN108239611A (en) | 2018-07-03 |
| CN108239611B CN108239611B (en) | 2021-06-08 |
Family
ID=62704889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611216477.9A Active CN108239611B (en) | 2016-12-26 | 2016-12-26 | Brevibacterium strain and method for in-situ remediation of heavy metal pollution of farmland by using same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108239611B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111378590A (en) * | 2018-12-27 | 2020-07-07 | 有研工程技术研究院有限公司 | Compound microbial agent and method for soil fertility improvement and ecological improvement by using same |
| CN112239737A (en) * | 2020-10-21 | 2021-01-19 | 深圳大学 | Marine bacterium and application thereof in remediation of marine heavy metal pollution |
| CN112553100A (en) * | 2019-09-26 | 2021-03-26 | 有研工程技术研究院有限公司 | Composite microbial agent and method for soil fertility improvement and ecological restoration of heavy metal-containing field by using same |
| CN112694984A (en) * | 2019-10-23 | 2021-04-23 | 国家电投集团远达环保工程有限公司重庆科技分公司 | Microbial compound inoculant for restoring mining area soil and use method thereof |
| CN115418325A (en) * | 2020-07-15 | 2022-12-02 | 北京工商大学 | Novel strain of Ningqing brevibacterium and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104593301A (en) * | 2015-01-15 | 2015-05-06 | 华南农业大学 | Bacillus muralis G1 as well as preparation method and application thereof |
-
2016
- 2016-12-26 CN CN201611216477.9A patent/CN108239611B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104593301A (en) * | 2015-01-15 | 2015-05-06 | 华南农业大学 | Bacillus muralis G1 as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| ANGELA VECCHIO ET AL.,: ""Heavy metal biosorption by bacterial cells"", 《FRESENIUS J ANAL CHEM》 * |
| 田美娟: ""深海重金属抗性菌的分离、鉴定以及菌株Brevibacterium sanguinis NCD-5(VI)还原机制的初探"", 《中国优秀硕士学位论文全文数据库基础科学辑》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111378590A (en) * | 2018-12-27 | 2020-07-07 | 有研工程技术研究院有限公司 | Compound microbial agent and method for soil fertility improvement and ecological improvement by using same |
| CN111378590B (en) * | 2018-12-27 | 2022-02-18 | 有研资源环境技术研究院(北京)有限公司 | Compound microbial agent and method for soil fertility improvement and ecological improvement by using same |
| CN112553100A (en) * | 2019-09-26 | 2021-03-26 | 有研工程技术研究院有限公司 | Composite microbial agent and method for soil fertility improvement and ecological restoration of heavy metal-containing field by using same |
| CN112694984A (en) * | 2019-10-23 | 2021-04-23 | 国家电投集团远达环保工程有限公司重庆科技分公司 | Microbial compound inoculant for restoring mining area soil and use method thereof |
| CN115418325A (en) * | 2020-07-15 | 2022-12-02 | 北京工商大学 | Novel strain of Ningqing brevibacterium and application thereof |
| CN115418325B (en) * | 2020-07-15 | 2023-10-20 | 北京工商大学 | Novel strain of Brevibacterium Armillariella and application thereof |
| CN112239737A (en) * | 2020-10-21 | 2021-01-19 | 深圳大学 | Marine bacterium and application thereof in remediation of marine heavy metal pollution |
| CN112239737B (en) * | 2020-10-21 | 2022-03-08 | 深圳大学 | Marine bacterium and application thereof in remediation of marine heavy metal pollution |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108239611B (en) | 2021-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104450552B (en) | A kind of sulfate reducing bacteria phosphate solubilizing bacteria and its application in combined repair of cadmium polluted soil | |
| CN108239611A (en) | One plant of brevibacterium and its method for heavy metals in farmland pollution in-situ immobilization | |
| CN111235076B (en) | Bacillus anethoides, microbial inoculum thereof and application thereof in heavy metal remediation field | |
| CN104630097B (en) | A kind of acidophilus sulfate reduction bacteria strain and its application | |
| CN105255782B (en) | There is fiber bacterium and the purposes of reducing power to Cr VI | |
| CN106493167B (en) | Bacillus licheniformis and microbial inoculum and their application and heavy metal-passivated method | |
| CN105219668B (en) | A kind of heavy metal-polluted soil activation microbial inoculum and the preparation method and application thereof | |
| CN111423996B (en) | Complex microbial inoculant, preparation method thereof and application thereof in soil remediation field | |
| CN106591277A (en) | Immobilized Bacillus having phosphate dissolving and heavy metal passivation functions and preparation method and application thereof | |
| CN110616171B (en) | Saline-alkali-resistant Pacific bacillus and viable bacteria preparation and application thereof | |
| CN102527713B (en) | Biological leaching restoring method for heavy metal polluted soil | |
| CN104371956B (en) | There is bacillus and the purposes of blocking effect to cadmium | |
| CN102391960A (en) | Arthrobacter chlorophenolicus L4 and application thereof | |
| CN112358974B (en) | Plant endophytic fungus epicoccum nigrum FZT214 and application thereof | |
| CN109055258A (en) | A kind of application of greedy copper bacterium and greedy copper bacteria preparation and greedy copper bacteria preparation in the repair of heavy metal contaminated soil | |
| CN106676049A (en) | Bacillus amyloliquefaciens strain and application thereof | |
| CN107815428A (en) | One plant of cadmium removes rhizobium KG2, microbial inoculum containing the rhizobium and application thereof | |
| CN108531424A (en) | One plant of efficient phosphorus-dissolution bacterium and its application for soil improvement | |
| CN105132314A (en) | Brevibacterium frigoritolerans YLX-3 and application thereof | |
| CN105670961B (en) | It is a kind of solve Phos plant growth-promoting bacterial strain NG-33 and its application | |
| CN109730088A (en) | A kind of improvement stony desertification ground microorganism formulation of plant growth and preparation method thereof | |
| CN104496725B (en) | Organic bacterial manure for lead zinc ore contaminated soil remediation and preparation method of organic bacterial manure | |
| CN104974947A (en) | Brevundimonas sp. subspecies strain SZ-22 and application thereof | |
| CN112189394A (en) | Three-dimensional improvement method for saline-alkali soil | |
| CN104789494B (en) | The method for improving turf salt-resistance using garbage compost microbial bacterial agent is strengthened |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190626 Address after: 101407 No. 11 Xingke East Street, Yanqi Economic Development Zone, Huairou District, Beijing Applicant after: Research Institute of engineering and Technology Co., Ltd. Address before: No. 2, Xinjie street, Xicheng District, Beijing, Beijing Applicant before: General Research Institute for Nonferrous Metals |
|
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210408 Address after: 101407 No.11, Xingke East Street, Yanqi Economic and Technological Development Zone, Huairou District, Beijing Applicant after: Youyan resources and Environment Technology Research Institute (Beijing) Co.,Ltd. Address before: 101407 No. 11 Xingke East Street, Yanqi Economic Development Zone, Huairou District, Beijing Applicant before: YOUYAN ENGINEERING TECHNOLOGY RESEARCH INSTITUTE Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |