CN108236741A - A kind of bion composite material oesophagus sticking patch - Google Patents
A kind of bion composite material oesophagus sticking patch Download PDFInfo
- Publication number
- CN108236741A CN108236741A CN201611221043.8A CN201611221043A CN108236741A CN 108236741 A CN108236741 A CN 108236741A CN 201611221043 A CN201611221043 A CN 201611221043A CN 108236741 A CN108236741 A CN 108236741A
- Authority
- CN
- China
- Prior art keywords
- sticking patch
- growth factor
- composite material
- oesophagus
- ontology
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
- A61L27/443—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with carbon fillers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/606—Coatings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/22—Materials or treatment for tissue regeneration for reconstruction of hollow organs, e.g. bladder, esophagus, urether, uterus
Abstract
The invention discloses a kind of bion composite material oesophagus sticking patch, it is characterised in that：By on sticking patch ontology, a surface coated in sticking patch ontology biological glue-line, the growth factor coating that is incorporated on another surface of sticking patch ontology forms, the material of the sticking patch ontology is polytetrafluorethylenano nano composite material, as the adherent face combined with esophageal wall, the surface for being combined with growth factor coating forms tube chamber face on surface coated with biological glue-line.The fistula mouth that the present invention does not need to can be realized tracheoesophageal fistula using supporting structure is repaired, and avoids various problems caused by being repaired in the prior art using stent；Meanwhile using patch structure, due to the sealing ability to tissue defect of biological glue-line, radiotherapy can be carried out to tracheoesophageal fistula；" bionical " change is carried out to tube chamber face by growth factor to modify, mucous membrane of esophagus epithelium sticking, regenerate and creeping on its surface can be promoted, it is fixed to play the role of fusion.
Description
Technical field
The present invention relates to a kind of medical equipments, and in particular to a kind of bion composite material for tracheoesophageal fistula treatment
Oesophagus sticking patch.
Background technology
Pernicious tracheoesophageal fistula (malignant tracheoesophageal fistula, MTEF) is a kind of derived from food
Pipe, etc. positions hypoploidy tumour severe complication, poor prognosis lacks effective radical-ability means, and there is an urgent need to find new to have
Imitate therapy.
Operation and the implantation of oesophagus covered stnet are current most common treatment means, but undesirable.Operative treatment is through choosing
The successful patients of MTEF selected, mean survival time (MST), reach 13 months, but mortality risk is up to 14.3% in art, and most
Patient can not be performed the operation due to local inflammation oedema, concurrent infection, advanced tumor.The implantation of oesophagus covered stnet is lacked there is also very much
It falls into：1. stent needs the normal esophageal wall for covering fistula proximal end and distal end at least 2cm, sufficiently wide so as to be fixedly secured.
However, above-mentioned condition sometimes and is not easy to meet.2. stent stimulates Esophageal Mucosa granulation hyperplasia, can not be taken after placing a period of time
Go out, while change the biological characteristics such as the original elasticity of oesophagus, retractility, the complication such as reflux esophagitis, pectoralgia, bleeding compared with
It is more.Lesion is located at that Upper Esophageal person's foreign body sensation is apparent, and patient is often difficult to be resistant to.3. lack the hand of follow-up effectively control tumour
Section, to the survival of patients time without being obviously prolonged, median survival interval only 8 weeks or so.
In addition, radiotherapy has critical role in advanced esophageal carcinoma treatment, but tracheoesophageal fistula is exactly once to put
The absolute contraindication for the treatment of even if after stenter to implant, leads to neoplasm necrosis since radiotherapy has, and fistula mouth further expands, and extends fistula
The risk of mouth healing time is also not suitable for continuing radiotherapy.
Invention content
The goal of the invention of the present invention is to provide a kind of bion composite material oesophagus sticking patch, when treating tracheoesophageal fistula most
In degree retain the original biological characteristics of oesophagus, while ensure that sticking patch is fixed with merging for tube wall.
To achieve the above object of the invention, the technical solution adopted by the present invention is：A kind of bion composite material oesophagus sticking patch,
By on sticking patch ontology, a surface coated in sticking patch ontology biological glue-line, be incorporated on another surface of sticking patch ontology
Growth factor coating form, the material of the sticking patch ontology is polytetrafluorethylenano nano composite material, coated with biological glue-line
Surface as the adherent face combined with esophageal wall, the surface for being combined with growth factor coating forms tube chamber face.
In above-mentioned technical proposal, the fistula oral area position of tracheoesophageal fistula is repaired by sticking patch, using polytetrafluoroethylene (PTFE)
Nanocomposite prepares sticking patch ontology, provides the biocompatibility of height, elasticity, toughness, have stretch-proof, acid and alkali-resistance,
Radiation-resistant characteristic.Biological glue-line is coated on a surface of sticking patch ontology, biogum is also known as Fibrin Glue (fibrin
Glue, FG), it is made of a variety of coercibility protein extracted in biological tissue, simulates the final step i.e. fiber of coagulation process
Proteinogen is transformed into fibrinous process.Surface coated with biological glue-line is utilized as the adherent face combined with esophageal wall
On the one hand biogum can be achieved sticking patch and be adhesively fixed, on the other hand, can be effectively sealing off tissue defect, promote wound healing,
Prevent tissue adhesion.Growth factor coating is combined on another surface of sticking patch ontology, by growth factor to tube chamber face
It carries out " bionical " change to modify, mucous membrane of esophagus epithelium sticking, regenerate and creeping on its surface can be promoted, it is fixed to play fusion
Effect.
In above-mentioned technical proposal, the polytetrafluorethylenano nano composite material is by polytetrafluoroethylene (PTFE), graphene and inorganic powder
It heats and prepares after the mixing cold moudling of end, the quality of the polytetrafluoroethylene (PTFE) is the 65%~90% of material gross mass, graphite
The quality of alkene is the 0.1%~10% of quality of materials, remaining is inorganic powder；In raw material, the grain size of the polytetrafluoroethylene (PTFE) is 20
~100 microns, the graphene crosses 200 mesh sieve, and the inorganic powder is calcium carbonate, talcum powder, two sulphur of 1~40 micron of grain size
Change one or more mixtures in molybdenum, graphite, glass fibre, carbon fiber.
In above-mentioned technical proposal, the sticking patch ontology is prepared using following method：
(1) polytetrafluoroethylene (PTFE), graphene and microfine inorganic powder are uniformly mixed, form mixed powder；
(2) mixed powder is placed in mold, the cold moudling under the pressure of 30~50MPa, and pressurize 2~5 minutes, demoulding
Obtain tablet；
(3) obtained sheet is warming up to 370~380 DEG C, heat preservation 2 hours is cold with 100~200 DEG C/h of heating rate
But sticking patch ontology is obtained.
In above-mentioned technical proposal, the preparation method of the growth factor coating is, using light coupling method in this body surface of sticking patch
Face adsorbing fiber Fibronectin, basic fibroblast growth factor or epidermal growth factor.
Since above-mentioned technical proposal is used, the present invention has following advantages compared with prior art：
1st, the present invention provides a kind of oesophagus sticking patch, by combining biogum on sticking patch ontology, the fixation of sticking patch is realized, thus
The fistula mouth for not needing to can be realized tracheoesophageal fistula using supporting structure is repaired, and is avoided and is made in the prior art using stent repairing
Into various problems；It, can be to tracheae due to the sealing ability to tissue defect of biological glue-line meanwhile using patch structure
Esophageal fistula carries out radiotherapy.
2nd, the material of sticking patch ontology is prepared in the present invention using polytetrafluorethylenano nano composite material, by polytetrafluoroethyl-ne
Graphene and inorganic powder are added in alkene, improves the mechanical property and wear-resisting property of material, while material has the life of height
Object compatibility, elasticity, toughness have stretch-proof, acid and alkali-resistance, radiation-resistant characteristic.
3rd, the present invention combines growth factor coating in tube chamber face, carries out " bionical " change to tube chamber face by growth factor and repaiies
Decorations, can promote mucous membrane of esophagus epithelium sticking, regenerate and creeping on its surface, and it is fixed to play the role of fusion.
Description of the drawings
Fig. 1 is the structure diagram of the embodiment of the present invention one.
Wherein：1st, sticking patch ontology；2nd, biological glue-line；3rd, growth factor coating；4th, adherent face；5th, tube chamber face.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and embodiments：
Embodiment one：It is shown in Figure 1, a kind of bion composite material oesophagus sticking patch, by sticking patch ontology 1, coated in sticking patch sheet
Biological glue-line 2 on one surface of body 1, the growth factor coating 3 being incorporated on another surface of sticking patch ontology 1 are formed,
The material of the sticking patch ontology 1 be polytetrafluorethylenano nano composite material, the surface coated with biological glue-line as with esophageal wall
With reference to adherent face 4, the surface for being combined with growth factor coating forms tube chamber face 5.
The sticking patch ontology is prepared using following method：
(1) graphene crosses 200 mesh sieve, is mixed with polytetrafluorethylepowder powder, the calcium carbonate superfine powder powder of about 25 microns of grain size, with
8000r/min rotating speeds intermittent stirring mixes 2 ~ 3min, forms mixed powder；Wherein the quality of polytetrafluorethylepowder powder is 85%, stone
The quality of black alkene is 6%, remaining is calcium carbonate powder.
(2) mixed powder is placed in mold, the cold moudling under the pressure of 34.5MPa, and pressurize 2~5 minutes, taken off
Film obtains tablet；The tablet that the mold shape designs the acquisition caused meets Anatomy of the esophagus physiological property.
(3) obtained sheet is warming up to 375 DEG C, is kept the temperature 2 hours, natural cooling with 100 DEG C/h of heating rate
To room temperature, sticking patch ontology is obtained.
The preparation method of the growth factor coating is, using light coupling method in sticking patch body surface adsorbing fiber adhesion egg
In vain, basic fibroblast growth factor or epidermal growth factor.The performance of the product obtained to illustrate the present embodiment, to this
The product of embodiment carries out performance measurement：
(1) bending stiffness of oesophagus sticking patch.
Experiment material resists the ability of its bending direction change in shape, referred to as bending stiffness.
(2) oesophagus sticking patch elastic recovery rate.
Test method：Sample carries out longitudinal elasticity deformation and transverse elasticity deformation is real respectively after sufficient standing bounces back
It tests.
Elongation (%)=（It is long after stretching-former long）/ former long × 100%.
Elastic recovery rate (%)=(long after length-recovery after stretching)/(long after stretching-former long) × 100%.
The elasticity (maximum flexibility elongation) of Woven stretch fabric can be defined as：Maximum flexibility elongation (%)=（Tension-like
Length under length-relaxed state under state）Length × 100% under/relaxed state.
After testing, the bending stiffness of oesophagus sticking patch, oesophagus sticking patch elastic recovery rate uniformly meet the requirement of sticking patch application.
(3) oesophagus sticking patch capability of resistance to radiation is tested：
Applicable line accelerator gives composite material oesophagus sticking patch 50Gy, 70Gy, 90Gy Radiotherapy dosimetry manufactured in the present embodiment,
Detect bending stiffness and the situation of change of elastic recovery rate before and after its radiotherapy.
The result shows that the sticking patch that the present invention obtains has good capability of resistance to radiation, it is adapted to the anti-requirement for penetrating treatment.
(4) biogum safety detection
By biogum and the acetic acid 1 of 20mM:30 are made into the liquid of 0.1mg/ml, with 50 μ l/cm2Amount be uniformly applied to 6cm2Culture
Ware surface is put in 4 DEG C of refrigerator overnights, and inoculation absorbed liquid before 1 hour, is put in 37 DEG C and dries, and sterile PBS liquid rinses 2 times, then
It is rinsed 1 time with cell culture fluid.Primary esophageal cells are detached, by 105The density of/ware, which is inoculated in, is coated with or is not coated with biogum
Culture dish surface, culture carefully removes culture medium after 6 days, and life is compared in row cell count after the acetate dissolution biogum of 20mM
Influence of the object glue to normal esophageal cell growth.
The result shows that the biogum that the present invention uses has biological safety.
(5) influence that basic fibroblast growth factor adheres to epithelial cell on constructed oesophagus sticking patch
1. original cuiture and the passage of animal esophageal epithelial cell
（a）Tissue block method：
Esophageal Mucosa is placed in the Hanks liquid of sterilizing and rinsed 2-3 times, remove tissue blood stains, it is big to be cut into about 1mm × 1mm × 1mm
Small tissue block is drawn fritter with elbow straw and is inputted in each culture bottle, put in bottom uniformly, fritter mutual distance
0.5cm or so adds in a little culture solution, puts in incubator, after 24 hours when tissue block adherent and start growth when add training again
Nutrient solution.Culture solution is RPMI, includes calf serum 10%, hydrogenation cortisone 0.4ug/ml, insulin 5ug/ml, epidermal growth factor
Sub- 1ng/ml, penicillin 100u/ml, streptomysin 100ug/ml, observe cell growth status daily.Exclude fibroblast.Training
Foster cell covers with bottle wall or carries out secondary culture when occupying 80% or so.
（b）Whole section of oesophagus (separation cell) cultivation：
Oesophagus is aseptically isolated, is rinsed repeatedly with the D-Hanks liquid or culture solution of 2-5ml, removes blood stains etc.；Ligation
Oesophagus both ends, injection enzymic digestion liquid I are allowed to full, abandon enzyme solution I after 10min, and injection enzyme solution II digests about 10min, digestion it is whole
Oesophagus is soaked in D-Hanks liquid during a（37℃、30min）；Oesophagus is splitted in longitudinal direction, and tissue surface is gently scraped with knife,
The epithelial cell of digestion is washed out with culture solution simultaneously, is collected in the culture solution of 10ml；Centrifuge 500rpm × 5min, after again with training
The cell suspension of 2 × 105-5 × 105/ml is made in nutrient solution suspension cell, repetition 1-2 times again；It is inoculated according to different requirements
In culture bottle or culture dish.
（c）Secondary culture：
The above-mentioned cell through Dissociated cell culture and explant-culture extremely should pass on confluent cultures ware or culture bottle in proliferation.1）It inhales
Culture solution is removed, is rinsed with D- Hanks liquid；2）It adds in trypsase-EDTA digestive juices to be digested, 50ul/cm2;3）It adds in
DMEM culture solutions containing 10% fetal calf serum terminate digestion；4）Gently piping and druming is allowed into suspension, and the epithelium for collecting digestion dispersion is thin
Born of the same parents；5）By 1:3 or 1:The cell of 4 dilution proportions dispersion, is passed on again.During secondary culture, add with some stimulating growth factors.
Such as：Insulin 10ug/ml, transferrins 5mg/ml hydrogenate cortisone 0.36mg/L, endothelial growth factors 7.5mg/ml, epidermis
Growth factor-2 5ug/ml, Sodium Pyruvate 20mmol/L etc..
（d）Cell differentiation culture：
The esophageal epithelial cell for detaching passage is planted on the coated support of collagen, is artificially formed liquid gas cultivation interface, with
Promote epithelial cell differentiation.1）By the epithelial cell digested with pancreatin passage plantation on the coated support of collagen, density 3 ×
105/cm2；2) DMEM containing 10%FBS is added in, culture is until Fusion Strain in the incubator；3）By the DMEM containing 10%FBS
It is replaced with the DMEM/F12 (1 containing 2%FBS:1) mixed-culture medium, while Culture liquid measure is controlled to expose epithelial cell free surface
In air（Living environment in similar epithelium of esophagus body）, culture solution dosage is 400ul/cm2.Cell can be in that multilayer is given birth at this time
It is long, there is cilium growth within about 3-6 weeks.
2. the influence that basic fibroblast growth factor sticks epithelial cell
(a) constructed oesophagus patching material is put into 96 orifice plates, adds 50mg/L collagen ivs or 50mg/L FN solution per hole
0.1ml, 4 DEG C overnight after be sucked out per hole solution, dry rear ultraviolet lamp vertical irradiation 3h disinfections.(b) the 34th generation oesophagus of culture glues
Film epithelial cell.Experimental group basic fibroblast growth factor conditioned medium, control group serum-free M199 culture solutions
18h is cultivated, 25 mg/L cycloheximides conditioned medium culture 1h exclude cell and glue so that cell to be inhibited to synthesize new ECM protein matter
Attached is caused by newly synthesizing ECM protein matter.(c) 625mg/L trypsase --- 100mg/LEDTA mixed liquor vitellophags,
It is 10 × 10 that density, which is made,8The cell suspension of/L.(d) cell is added on 96 orifice plate grapheme materials, per hole 0.1ml, be put into
37℃CO215min is cultivated in incubator.(e) D Hanks liquid rinses three times, absorbs non-attached cell.(f) add 50mg/ per hole
LFDA0.1ml, after being stored at room temperature 30min, is sucked out FDA, is rinsed three times with D Hanks liquid respectively again per hole.(g) add 1% per hole
Triton X-100 0.1ml, 4 DEG C overnight after, rinse the grapheme material in each hole with D Hanks liquid 0.1ml, and by material
It takes out.(h) it is continuously surveyed per the fluorescence intensity at the 485nm wavelength of hole on Fluorescence Scanner.(i) experiment is repeated twice, according to institute
It surveys fluorescence optical density value and the cell number accordingly sticked is obtained in standard curve, then go out cell adhesion rate according to the following formula,
Statistical procedures are examined with t.Cell adhesion rate=adherent cell number/addition cell number × 100%.
The result shows that Epithelial Cell Adhesion can be made on oesophagus sticking patch using basic fibroblast growth factor, effectively
Realize the biomimetic activation in tube chamber face.
Claims (4)
1. a kind of bion composite material oesophagus sticking patch, it is characterised in that：By sticking patch ontology, a table coated in sticking patch ontology
Biological glue-line on face, the growth factor coating being incorporated on another surface of sticking patch ontology are formed, the sticking patch ontology
Material is polytetrafluorethylenano nano composite material, and the surface coated with biological glue-line is as the adherent face combined with esophageal wall, knot
The surface that closing has growth factor coating forms tube chamber face.
2. bion composite material oesophagus sticking patch according to claim 1, it is characterised in that：The polytetrafluorethylenano nano
Composite material is prepared by being heated after polytetrafluoroethylene (PTFE), graphene and inorganic powder mixing cold moudling, the polytetrafluoroethyl-ne
The quality of alkene is the 65%~90% of material gross mass, and the quality of graphene is the 0.1%~10% of quality of materials, remaining is inorganic powder
End；In raw material, the grain size of the polytetrafluoroethylene (PTFE) is 20~100 microns, and the graphene crosses 200 mesh sieve, the inorganic powder
It is one or more in the calcium carbonate of 1~40 micron of grain size, talcum powder, molybdenum disulfide, graphite, glass fibre, carbon fiber
Mixture.
3. bion composite material oesophagus sticking patch according to claim 2, it is characterised in that：Under the sticking patch ontology uses
Row method prepares：
(1) polytetrafluoroethylene (PTFE), graphene and microfine inorganic powder are uniformly mixed, form mixed powder；
(2) mixed powder is placed in mold, the cold moudling under the pressure of 30~50MPa, and pressurize 2~5 minutes, demoulding
Obtain tablet；
(3) obtained sheet is warming up to 370~380 DEG C, heat preservation 2 hours is cold with 100~200 DEG C/h of heating rate
But sticking patch ontology is obtained.
4. bion composite material oesophagus sticking patch according to claim 1, it is characterised in that：The growth factor coating
Preparation method is, using light coupling method sticking patch body surface adsorbing fiber Fibronectin, basic fibroblast growth factor,
Or epidermal growth factor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611221043.8A CN108236741A (en) | 2016-12-26 | 2016-12-26 | A kind of bion composite material oesophagus sticking patch |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611221043.8A CN108236741A (en) | 2016-12-26 | 2016-12-26 | A kind of bion composite material oesophagus sticking patch |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108236741A true CN108236741A (en) | 2018-07-03 |
Family
ID=62701433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611221043.8A Pending CN108236741A (en) | 2016-12-26 | 2016-12-26 | A kind of bion composite material oesophagus sticking patch |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108236741A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385669A (en) * | 2007-09-12 | 2009-03-18 | 中国科学院金属研究所 | Anti-coagulation stainless steel coronary arterial bracket and uses thereof |
| CN201316331Y (en) * | 2008-10-15 | 2009-09-30 | 周星 | Biologically induced composite artificial esophagus |
| CN102731942A (en) * | 2012-06-13 | 2012-10-17 | 浙江大学 | Preparation method of talc filled PTFE composite material |
| CN103263693A (en) * | 2012-12-04 | 2013-08-28 | 中国医学科学院生物医学工程研究所 | Preparation method and use of immobilized antimicrobial drug hernia repair patch |
| CN105199278A (en) * | 2015-10-27 | 2015-12-30 | 巨轮智能装备股份有限公司 | Graphene/ polytetrafluoroethylene composite material and method for preparing vulcanizer friction ring from same |
| CN105555299A (en) * | 2013-04-22 | 2016-05-04 | 西兰丁姆医疗公司 | Fibrinogen-based tissue adhesive patches |
-
2016
- 2016-12-26 CN CN201611221043.8A patent/CN108236741A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385669A (en) * | 2007-09-12 | 2009-03-18 | 中国科学院金属研究所 | Anti-coagulation stainless steel coronary arterial bracket and uses thereof |
| CN201316331Y (en) * | 2008-10-15 | 2009-09-30 | 周星 | Biologically induced composite artificial esophagus |
| CN102731942A (en) * | 2012-06-13 | 2012-10-17 | 浙江大学 | Preparation method of talc filled PTFE composite material |
| CN103263693A (en) * | 2012-12-04 | 2013-08-28 | 中国医学科学院生物医学工程研究所 | Preparation method and use of immobilized antimicrobial drug hernia repair patch |
| CN105555299A (en) * | 2013-04-22 | 2016-05-04 | 西兰丁姆医疗公司 | Fibrinogen-based tissue adhesive patches |
| CN105199278A (en) * | 2015-10-27 | 2015-12-30 | 巨轮智能装备股份有限公司 | Graphene/ polytetrafluoroethylene composite material and method for preparing vulcanizer friction ring from same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107281550B (en) | Preparation method of co-crosslinked double-network hydrogel scaffold for promoting cartilage injury repair | |
| CN101361989B (en) | Double membrane tissue patching material and preparation method thereof | |
| CN101361990B (en) | Double layer artificial skin and preparation method thereof | |
| Pode et al. | The mechanism of human bladder tumor implantation in an in vitro model | |
| CN101366976B (en) | Humanized heterogenous cell epimatrix material and preparation method thereof | |
| CN101366977B (en) | Tissue mending material with biological activity and preparation method thereof | |
| CN107802890A (en) | Bioengineered tissue constructs and its preparation and application | |
| CN101366979B (en) | Tissue patch and preparation method thereof | |
| CN101318032A (en) | Small-diameter tissue engineering artificial blood vessel and preparation method thereof | |
| CN102038564A (en) | Supporting frame capable of being taken out | |
| CN110665050B (en) | Biological adhesive and preparation method thereof | |
| TW200819150A (en) | Skin substitutes, preparation methods and uses thereof | |
| CN103705984A (en) | Preparation method and application of collagen scaffold composite bone marrow-derived mesenchymal stem cells (BMSCs) | |
| CN105194735A (en) | Acellular biological amnion and preparation method of genipin crosslinked acellular biological amnion | |
| CN100368534C (en) | Bioderived amnion, composite bioderived amnion and its preparation method | |
| CN105311678A (en) | Complex hernia patch and preparing method and application thereof | |
| CN201316331Y (en) | Biologically induced composite artificial esophagus | |
| CN104971382B (en) | The mounted artificial active mass of wound and its construction method that a kind of use serum-free and ox pituitary extract nutrient solution are built | |
| CN101721262A (en) | Tissue engineering combined human body lumen succedaneum | |
| CN201316330Y (en) | Combined tissue-engineered coelomic duct substitute | |
| CN107050529B (en) | A kind of uterine cavity built-in object, preparation method and applications | |
| CN108236741A (en) | A kind of bion composite material oesophagus sticking patch | |
| CN103054628A (en) | Method for fast constructing and repairing skin defects by tissue engineering skins | |
| CN110229783A (en) | A method of stem cell diaphragm is prepared based on warm ware | |
| CN108350420A (en) | The method for cultivating cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180703 |