CN108226124A - A kind of protein Raman microscratch detector based on database quick-searching - Google Patents
A kind of protein Raman microscratch detector based on database quick-searching Download PDFInfo
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- CN108226124A CN108226124A CN201711430907.1A CN201711430907A CN108226124A CN 108226124 A CN108226124 A CN 108226124A CN 201711430907 A CN201711430907 A CN 201711430907A CN 108226124 A CN108226124 A CN 108226124A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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Abstract
The present invention relates to a kind of protein Raman microscratch detectors based on database quick-searching, including database module, analysis shows that six module, laser module, lens module, Raman lens and detection probe parts, wherein, laser module, for generating laser；Raman lens are beaten for laser is converged in surface enhanced probe；Database module is the Raman spectrum library of micrometer trace sample, is used as the standard of comparing；Collected Raman spectral information is carried out data with database and quickly compared, forms quantitative and qualitative analysis and the display of rapid qualitative index by Raman spectrum display module.Surface enhanced probe is directly detected with the contact of micro article, and main body is optical fiber, and nano metal silver coating is formed with by surface diposition in optical fiber surface.
Description
Technical field
The invention belongs to the fast inspection technical fields of microscratch sample.
Background technology
Since phase late 1960s till now, database technology has been developed more than 30 years.At this more than 30 years
In course, people achieve brilliant achievement in the theoretical research and system development of database technology, and have begun
Further investigation to advanced database system.Database Systems have become the important component of modem computer systems.
With the development of computer technology and network communication technology, database technology has become in information-intensive society to largely counting
It is the basis of internet information management system according to the important technical and software technology for carrying out Organization And Management.Database skill
Art is the important component of Modern information science and technology, is the core of computer digital animation and information management system.Number
According to library technical research and during solving the problems, such as computer information processing, mass data is effectively organized and is stored, in data
Data storage redundancy is reduced in the system of library, data sharing is realized, ensure data safety and efficiently retrieves data and processing number
According to.
In recent years, occur the sphygmomanometer and blood pressure instrument of New Generation of Intelligent in society so that take in field of biological detection
Some major progresses, Surface Enhanced Raman Scattering Spectrum it is quick compare one that analysis is referred to as in the field it is important prominent
It is broken.But in biology, medical research, traditional biosensor volume is larger, is simply possible to use in tissue, cell suspension etc.
Measurement, when detecting the size reduction of sample to micron order, such as detect living cells or during other subcellular components, in real time, standard
Really, interference-free is measured in sample after chemical and natural component, and analysis and comparing become extremely difficult.
Invention content
Present invention aims at a kind of high sensitivity is provided and the protein Raman microscratch based on database quick-searching is examined
Instrument is surveyed, probe manufacturing technique is improved, and data quick-searching mode and Raman microscratch fast detector are combined, passes through
Detection probe detects microscratch sample to be measured, and laser sends out laser, obtains Raman signal, and then be transferred in detector ontology
Analysis shows that Raman spectrum in module, with database carries out rapid data compares, form the quantitative and qualitative point of quantizating index
Analysis.Technical solution is as follows：
A kind of protein Raman microscratch detector based on database quick-searching, including database module, analysis shows that
Six module, laser module, lens module, Raman lens and detection probe parts, wherein,
Laser module, for generating laser；
Raman lens are beaten for laser is converged in surface enhanced probe；
Database module is the Raman spectrum library of micrometer trace sample, is used as the standard of comparing；
Collected Raman spectral information is carried out data with database and quickly compared, formed by Raman spectrum display module
The quantitative and qualitative analysis of rapid qualitative index and display.
Surface enhanced probe is directly detected with the contact of micro article, and main body is optical fiber, passes through surface in optical fiber surface
Deposition technique is formed with nano metal silver coating, can reach the acquisition of the Raman spectrum to trace level sample to be tested；Surface enhanced
The production method of probe is as follows：
1) solid core fibres are chosen；
2) solid core fibres surface is roughened by electrochemical method；
3) so that Ag is coated in the solid core fibres surface Jing Guo roughening treatment, nano level protrusion is formed.
The present invention can realize the quantitative and qualitative analysis of the rapid qualitative index to microscratch article Raman spectrum, suitable for people
Body protein quality detection has the advantages that detection speed is fast, accuracy is high, compact structure, quick light.
Description of the drawings
Fig. 1 is testing result schematic diagram of the present invention；
Fig. 2 is the formation schematic diagram of surface enhanced probe of the present invention；
Fig. 3 is the portable Raman microscratch detector structure diagram of the present invention；
Fig. 4 is the portable Raman microscratch detector testing process schematic diagram of the present invention.
Specific embodiment
Below according to Figure of description, technical scheme of the present invention is introduced in detail.
Protein Raman microscratch detector structure the present invention is based on database quick-searching is as shown in Fig. 2, as figure can be with
Find out the portable Raman microscratch quickly detect detector be by database module 1, analysis shows that module 2, laser module 3,
Six lens module 4, Raman lens 5, detection probe major parts form.Wherein database module is micrometer trace sample
Raman spectrum library is used as the standard of comparing.Analysis shows that module is ccd detector, for showing the next Raman of acquisition
Information after spectral information and data analysis realizes that the data with database quickly compare, and form quantifying for rapid qualitative index
Qualitative analysis.For laser module specifically using 785nm lasers, Raman lens are beaten after laser to be converged to a dot
In detection probe.Detection probe is directly detected with the contact of micro article, and material is using fiber optic materials, and surface is to pass through
The nano metal silver coating that surface diposition is formed, and be the active table of Raman enhancing by Electrochemical roughening processing
Face；It, can after target molecule is recognized when the upper Raman molecular of metal surface modification and the ligand such as antibody combined with target molecule
Raman signal is obtained, Raman spectrum will be acquired to micro-example, and realize the quick detection of microscratch sample
In order to illustrate more clearly of the operation principle of the present invention, the detection probe of front end is first introduced.As shown in figure 3, detection
Material is solid core fibres material used by probe, is first roughened with solid core fibres probe by electrochemical method,
It is put into after having handled in the glass tubule for filling Ag colloidal sols, at the uniform velocity lifts solid core fibres probe upwards, so that Ag is coated in
Optical fiber probe surface forms nano level protrusion, improves the surface-enhanced Raman factor, enhances the Raman signal detected, realizes
The quick detection of portable Raman detector.
Protein Raman microscratch detector flow diagram the present invention is based on database quick-searching is as shown in Figure 4.When
When detection probe is contacted with microscratch sample, laser is sent out from 785nm lasers to lens module, by Raman lens, by laser
It is beaten in detection probe after converging to a dot.The detecting probe surface coated with nano Ag metal layers are modified when metal surface and are pulled up
Graceful molecule and the ligand such as antibody combined with protein molecule, can obtain Raman signal after protein molecule is recognized；It is tested
Sample is generated Raman signal after laser excitation, and is collected by Raman lens, the Raman signal after collection by lens module into
As in database module, by carrying out rapid comparison with the data in database module, forming quantifying for rapid qualitative index
Qualitative analysis analysis shows that shown in module, is stored in internal storage unit.And it external can be deposited in detector ontology
Equipment is stored up, the Raman spectral information tested is exported, convenient for the analysis and management in later stage.
Claims (1)
1. a kind of protein Raman microscratch detector based on database quick-searching, including database module, analysis shows that mould
Six block, laser module, lens module, Raman lens and detection probe parts, wherein,
Laser module, for generating laser；
Raman lens are beaten for laser is converged in surface enhanced probe；
Database module is the Raman spectrum library of micrometer trace sample, is used as the standard of comparing；
Collected Raman spectral information is carried out data with database and quickly compared, formed quick by Raman spectrum display module
The quantitative and qualitative analysis of quantizating index and display.
Surface enhanced probe is directly detected with the contact of micro article, and main body is optical fiber, is deposited in optical fiber surface by surface
Technology is formed with nano metal silver coating, can reach the acquisition of the Raman spectrum to trace level sample to be tested；Surface enhanced probe
Production method it is as follows：
1) solid core fibres are chosen；
2) solid core fibres surface is roughened by electrochemical method；
3) so that Ag is coated in the solid core fibres surface Jing Guo roughening treatment, nano level protrusion is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711430907.1A CN108226124A (en) | 2017-12-26 | 2017-12-26 | A kind of protein Raman microscratch detector based on database quick-searching |
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| CN201711430907.1A CN108226124A (en) | 2017-12-26 | 2017-12-26 | A kind of protein Raman microscratch detector based on database quick-searching |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114264641A (en) * | 2020-09-16 | 2022-04-01 | 中国科学院过程工程研究所 | Method for detecting concentration of trace ionic liquid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104568905A (en) * | 2015-01-19 | 2015-04-29 | 东南大学 | Three-dimensional code biological detection chip based on surface-enhanced Raman scattering (SERS) microflow platform as well as preparation method and detection method of biological detection chip |
| CN106770170A (en) * | 2016-12-29 | 2017-05-31 | 天津大学 | Can modularization apolegamy assembling Raman microscratch fast detector |
-
2017
- 2017-12-26 CN CN201711430907.1A patent/CN108226124A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104568905A (en) * | 2015-01-19 | 2015-04-29 | 东南大学 | Three-dimensional code biological detection chip based on surface-enhanced Raman scattering (SERS) microflow platform as well as preparation method and detection method of biological detection chip |
| CN106770170A (en) * | 2016-12-29 | 2017-05-31 | 天津大学 | Can modularization apolegamy assembling Raman microscratch fast detector |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114264641A (en) * | 2020-09-16 | 2022-04-01 | 中国科学院过程工程研究所 | Method for detecting concentration of trace ionic liquid |
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Application publication date: 20180629 |
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