CN108225884A - A kind of combined degradation method of zearalenone and ochratoxin A in solution - Google Patents
A kind of combined degradation method of zearalenone and ochratoxin A in solution Download PDFInfo
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- CN108225884A CN108225884A CN201711345112.0A CN201711345112A CN108225884A CN 108225884 A CN108225884 A CN 108225884A CN 201711345112 A CN201711345112 A CN 201711345112A CN 108225884 A CN108225884 A CN 108225884A
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- G—PHYSICS
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses the combined degradation methods of zearalenone in a kind of solution and ochratoxin A, belong to mycotoxin and environmental technology field in degradation food.The present invention is the new technique of mycotoxin joint abatement link in the crops such as grain, its main feature is that carrying out irradiation-induced degradation to the mixed solution of ZEN and OTA using the electron beam that electron accelerator generates.Experimentation confirms：ZEN and OTA significant effects in electron beam irradiation degradation mixed solution.When ZEN and OTA concentration is respectively 1.0 and 0.5 μ g/mL in mixed solution, irradiation-induced degradation most pronounced effects, degradation rates of the ZEN and OTA in acetonitrile solution is respectively 86.70% and 93.35% during 12kGy.Illustrate that electron beam irradiation changes or eliminate the toxic sites in ZEN and OTA molecules, achieved the purpose that efficient degradation.
Description
Technical field
It the present invention relates to the use of the side of zearalenone and ochratoxin A in electron beam irradiation while degraded solutions
Method belongs to mycotoxin and environmental technology field in degradation food.
Background technology
Zearalenone (Zearalenone, ZEN) and ochratoxin A (Ochratoxin A, OTA) be respectively by
The toxic secondary metabolic product that Fusarium and aspergillus and Penicillium generate, it is worldwide widely distributed, it causes a large amount of
Safety of human and livestock's accident and economy, environmental loss.About the cereal crops such as mycotoxin of corn, barley and wheat pollution document
In report, ZEN and OTA are existed simultaneously more.Research data shows that ZEN and OTA is respectively provided with stronger immunotoxicity, and ZEN is also in addition
With certain genetoxic, genotoxicity and cytotoxicity, OTA also has stronger kidney and hepatotoxicity, to a certain degree
On can teratogenesis, carcinogenic and mutagenesis.
There are many biodegrading process of existing ZEN and OTA, including physical methods, the changes such as alkaline process and oxidizing process such as heating, adsorbing
Method and using the biological methods such as microbial degradation, but exist or degradation efficiency is low or has reagent residual or makes
It is notable or the problems such as degradation of high cost, condition requirement is harsh into matrix nutrition and flavor loss, and be for single toxin into
Row degradation, is not suitable for the pollution condition that more toxin coexist.
Electron beam irradiation is that the electron beam generated using electron accelerator irradiates food material, reaches desinsection, kills
The purpose of bacterium, fresh-keeping and attenuation, acting on ZEN and OTA can make lps molecule toxic sites that fracture, transformation occur, and make its forfeiture
Or toxicity is reduced, there are the outstanding advantages such as capacity usage ratio is high, easy to operate, safe to use, be a safely and effectively green
Processing method.
Invention content
In view of the above-mentioned problems existing in the prior art, the applicant provides zearalenone and reddish brown song in a kind of solution
The combined degradation method of mould toxin A.The problem of present invention is existed simultaneously for the ZEN in Mycotoxins in Cereal and OTA is led to
It crosses to toxin standard items in the parameter optimization of different solvents, various concentration proportioning and different irradiation dose, proposes to utilize electron beam
Irradiate the theoretical method of ZEN and OTA in combined degradation solution.
Technical scheme is as follows：
A kind of combined degradation method of zearalenone and ochratoxin A in solution, includes the following steps：
(1) mixed solution that ZEN and OTA initial concentrations are respectively lower than the ZEN and OTA of 5 μ g/mL and 1 μ g/mL is prepared；
(2) mixed solution of the electron beam irradiation ZEN and OTA generated using electron beam irradiation, mixed solution of degrading
In toxin；
(3) ZEN the and OTA contents in mixed solution after irradiation are measured respectively using high performance liquid chromatography.
Preferably, in a kind of solution in the combined degradation method of zearalenone and ochratoxin A, institute
State in step (1) ZEN/OTA initial concentrations in ZEN and OTA mixed solutions be respectively 5.0/0.1,2.0/0.2,1.0/0.5,
0.5/1.0μg/mL。
Preferably, in a kind of solution in the combined degradation method of zearalenone and ochratoxin A, institute
The solvent for stating ZEN and OTA mixed solutions in step (1) is respectively methanol and acetonitrile.
Preferably, in a kind of solution in the combined degradation method of zearalenone and ochratoxin A, institute
The electron-beam dose for stating irradiation ZEN and OTA mixed solutions in step (2) is 3~12kGy.
The present invention is beneficial to be had technical effect that：
The present invention devises a kind of combined degradation method of zearalenone and ochratoxin A in solution.The present invention
The mixing methanol and acetonitrile solution of ZEN and OTA are irradiated using electron beam, oxidisability can be generated after lps molecule is irradiated
Extremely strong free radical, free radical further attack phenyl ring, unsaturated bond and active site in lps molecule, lead to lps molecule
Degradation.Meanwhile the present invention is by changing the concentration proportioning of ZEN and OTA and electron beam irradiation dosage, ZEN and OTA in mixed solution
Degradation rate in dose dependent, under 9~12kGy irradiation doses, in acetonitrile solvent, the degradation effect of two kinds of toxin is most
It is good.Easy to operate, significant effect of the invention, ZEN and OTA that can be in efficient degradation mixed solution, two kinds of toxin degradation rates reach
93% is more up to more than 85%, OTA degradation rates.
Description of the drawings
Fig. 1 is the influence that various concentration irradiates effect with comparison ZEN and OTA mixed solutions in the embodiment of the present invention 1.
Fig. 2 is the influence that different solvents irradiate ZEN and OTA mixed solutions effect in the embodiment of the present invention 2.
Fig. 3 is the influence that different irradiation doses irradiate ZEN and OTA mixed solutions effect in the embodiment of the present invention 3.
Specific embodiment
With reference to the accompanying drawings and examples, the present invention is specifically described.
Embodiment 1, various concentration are with the influence for comparing ZEN and OTA mixed solutions irradiation effect
Under room temperature, a certain amount of ZEN and OTA standard reserving solutions is taken to mix respectively, nitrogen be blown to it is dry after, answered using acetonitrile
It is molten, the working solution that ZEN/OTA concentration proportionings are respectively 5.0/0.1,2.0/0.2,1.0/0.5 and 0.5/1.0 μ g/mL is obtained, is adopted
Radiation treatment is carried out with the electron beam of 9kGy.
Interpretation of result：
As shown in Figure 1, under various concentration proportioning, ZEN and OTA achieves significant degradation effect, OTA drops in working solution
Solution rate reaches more than 90%, ZEN degradation rates also above 65%, when concentration proportioning is 1.0/0.5 μ g/mL, in working solution ZEN and
OTA synthesis degradation effects are slightly above other groups.
The influence of embodiment 2, different solvents to ZEN and OTA mixed solutions irradiation effect
Under room temperature, a certain amount of ZEN and OTA standard reserving solutions is taken to mix respectively, nitrogen be blown to it is dry after, acetonitrile is respectively adopted
It is redissolved with methanol, obtains the working solution that ZEN/OTA concentration proportionings are 1.0/0.5 μ g/mL, irradiated using the electron beam of 9kGy
Processing.
Interpretation of result：
As shown in Fig. 2, the ZEN and OTA in different solvents working solution achieve significant degradation results, acetonitrile solvent
In, OTA and ZEN degradation rates are more up to 90.61% and 74.67%.Reason is that methanol is hole trapping agents, can capture hydrogen
Oxygen radical and aqueous electron have certain inhibiting effect to toxin degradation, and acetonitrile solvent is irradiated at this dose to produce one
Quantitative free radical takes part in the degradation process of toxin, therefore ZEN and OTA degradation rates are significantly better than methanol solvate in acetonitrile solvent
In.
The influence of embodiment 3, different irradiation doses to ZEN and OTA mixed solutions irradiation effect
Under room temperature, a certain amount of ZEN and OTA standard reserving solutions is taken to mix respectively, nitrogen be blown to it is dry after, answered using acetonitrile
It is molten, the working solution that ZEN/OTA concentration proportionings are 1.0/0.5 μ g/mL is obtained, is carried out at irradiation using the electron beam of 3~12kGy
Reason.
Interpretation of result：
As shown in figure 3, under 3kGy dosage, ZEN and OTA degradation rates are essentially identical, and with the increase of irradiation dose,
OTA degradation rates are gradually higher than ZEN, and during 12kGy, OTA degradation rates reach more than 93%, ZEN degradation rates and are also up to 86.70%.It is former
Because being in OTA molecular structures containing relatively large number of polar sites, be easy to electron beam irradiation degradation, ZEN degradation rates are in after 9kGy
Certain speedup, the reason is that, under higher irradiation dose, acetonitrile is irradiated to produce free radical, promotes the further drop of ZEN
Solution, and OTA toxin is since the overwhelming majority is degraded at this time, in solution remaining content it is too low so that the free radical that generates of acetonitrile not
It is obviously promoted the further degradation of OTA.
The present invention devises a kind of combined degradation method of zearalenone and ochratoxin A in solution.The present invention
The mixing methanol and acetonitrile solution of ZEN and OTA are irradiated using electron beam, oxidisability can be generated after lps molecule is irradiated
Extremely strong free radical, free radical further attack phenyl ring, unsaturated bond and active site in lps molecule, lead to lps molecule
Degradation.Meanwhile the present invention is by changing the concentration proportioning of ZEN and OTA and electron beam irradiation dosage, ZEN and OTA in mixed solution
Degradation rate in dose dependent, under 9~12kGy irradiation doses, in acetonitrile solvent, the degradation effect of two kinds of toxin is most
It is good.Easy to operate, significant effect of the invention, ZEN and OTA that can be in efficient degradation mixed solution, two kinds of toxin degradation rates reach
93% is more up to more than 85%, OTA degradation rates.
Claims (7)
1. the method for toxin is mixed in a kind of combined degradation solution using electron beam irradiation, which is characterized in that the mixing to toxin
Solution uses electron beam irradiation degradation treatment.
2. according to the method described in claim 1, it is characterized in that, toxin in the mixed solution for zearalenone and
Ochratoxin A.
3. according to the method described in claim 1, it is characterized in that, the method includes following specific steps：
(1) toxin mixed solution is prepared using toxin standard reserving solution；
(2) the electron beam irradiation toxin mixed solution generated using electron beam irradiation；
(3) content of toxins in mixed solution is measured respectively using high performance liquid chromatography.
4. according to the method described in claim 3, it is characterized in that, toxin mixed solution is using different molten in the step (1)
Agent is matched with various concentration and prepared.
5. according to the method described in claim 3, it is characterized in that, electron beam irradiation toxin mixed solution in the step (2)
It is carried out with different irradiation doses.
6. according to the method described in claim 3, it is characterized in that, toxin mixed solution is using a certain amount of in the step (1)
Zearalenone and the mixing of ochratoxin A standard reserving solution, nitrogen be blown to it is dry after, methanol is respectively adopted and acetonitrile redissolves, obtains
It is respectively the working solution of 5.0/0.1,2.0/0.2,1.0/0.5 and 0.5/1.0 μ g/mL to ZEN/OTA concentration proportionings.
7. according to the method described in claim 3, it is characterized in that, electron beam irradiation toxin mixed solution in the step (2)
Dosage be 3~12kGy.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103599619A (en) * | 2013-11-15 | 2014-02-26 | 中国农业科学院农产品加工研究所 | Method for improving degradation effect of ochratoxin A (OTA) in solution by using electron beam irradiation |
| CN103645265A (en) * | 2013-12-23 | 2014-03-19 | 江苏省农业科学院 | Irradiation degradation treatment method for fumitremorgin and ochratoxin |
| WO2014056007A2 (en) * | 2012-10-09 | 2014-04-17 | Erber Aktiengesellschaft | Enzyme preparation for transforming ergopeptines, method therefor and animal feed and feed supplement or silage supplement containing said preparation |
-
2017
- 2017-12-15 CN CN201711345112.0A patent/CN108225884B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014056007A2 (en) * | 2012-10-09 | 2014-04-17 | Erber Aktiengesellschaft | Enzyme preparation for transforming ergopeptines, method therefor and animal feed and feed supplement or silage supplement containing said preparation |
| CN103599619A (en) * | 2013-11-15 | 2014-02-26 | 中国农业科学院农产品加工研究所 | Method for improving degradation effect of ochratoxin A (OTA) in solution by using electron beam irradiation |
| CN103645265A (en) * | 2013-12-23 | 2014-03-19 | 江苏省农业科学院 | Irradiation degradation treatment method for fumitremorgin and ochratoxin |
Non-Patent Citations (1)
| Title |
|---|
| XIAOHU LUO ET AL: ""Effects of Electron Beam Irradiation on Zearalenone and Ochratoxin A in Naturally Contaminated Corn and Corn Quality Parameters"", 《TOXINS》 * |
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Inventor after: Luo Xiaohu Inventor after: Chen Zhengxing Inventor after: Li Ke Inventor after: Wang Ren Inventor after: Wang Li Inventor after: Li Yanan Inventor after: Du Zhihong Inventor after: Pan Lihong Inventor before: Chen Zhengxing Inventor before: Luo Xiaohu Inventor before: Li Ke Inventor before: Wang Ren Inventor before: Wang Li Inventor before: Li Yanan Inventor before: Du Zhihong Inventor before: Pan Lihong |
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