CN108220177A - Being isolated from fulvic acid has the lactic acid bacteria of probiotic active - Google Patents
Being isolated from fulvic acid has the lactic acid bacteria of probiotic active Download PDFInfo
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- CN108220177A CN108220177A CN201611135319.0A CN201611135319A CN108220177A CN 108220177 A CN108220177 A CN 108220177A CN 201611135319 A CN201611135319 A CN 201611135319A CN 108220177 A CN108220177 A CN 108220177A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
Being isolated from fulvic acid has the lactic acid bacteria of probiotic active, which is isolated from fulvic acid solution.On lactic acid bacteria MRS Agar Platings, after 37 DEG C are cultivated 48 hours, 25 45 DEG C of growth pH of temperature：pH 6.0‑8.0；It is amphimicrobian, acidproof, for the antibacterial activity of pathogenic bacteria：There is antibacterial activity for Escherichia coli, staphylococcus aureus, salmonella typhimurium, Bacillus cercus, Listeria monocytogenes and proteus mirabilis.The present invention also provides a kind of compositions of the lactic acid bacteria comprising effective dose.With excellent acid resistance antibacterial activity, the good candidate as the seed for producing various fermented dairy products and other fermented foods is become.
Description
Technical field
Invention is related to a kind of probiotic lactobacillus, more precisely, is related to a kind of new lactic acid bacteria for belonging to lactobacillus, institute
It states lactic acid bacteria and is isolated from fulvic acid, and with excellent probiotic active, such as acid resistance, antibacterial activity.
Background technology
Humic acid substance is present in peat and lignite in large quantities.Humic acid substance active function groups enrich, and have
The performances such as certain ion exchange and absorption.Fulvic acid (fulvic acid) molecule is smaller and soluble easily in water with molecular weight
The characteristics of, since molecular weight is relatively small compared with humic acid, function admirable, since the characteristics of fulvic acid is above-mentioned leads to it
Higher price, the preparation and application of fulvic acid are also increasingly studied by domestic and foreign scholars.The method for preparing fulvic acid is main
There are extraction method, fermentation method, electro-osmosis method three categories.Wherein, extraction method includes alkali-soluble acid analysis method, strong acid extraction process, sulfuric acid third again
Four kinds of ketone method, ion-exchange-resin process methods.Since fulvic acid is the water soluble part in humic acid, its smaller molecule in addition
Amount has its special performance and use value.Oxygen content is relatively more in fulvic acid molecule and carbon content is relatively fewer, knot
Containing phenyl ring, thick phenyl ring and multiclass heterocycle in structure, and be connected between each ring by bridged bond, phenolic hydroxyl group, carboxyl, methoxyl group, ketone group,
The multiclass such as sulfonic group functional group is present in ring and branch, and therefore, fulvic acid compared with humic acid there is stronger cation to hand over
Transducing power, chelating ability and adsorption capacity.
At present fulvic acid in work, agriculture, cure, the various aspects such as herd and have extensive purposes.Industrial aspect:Mainly use
In heavy metal-polluted water process, the harmful metal ion in waste water can be removed as the adsorbent of metal ion;Agriculture aspect:
With improving soil quality, promote plant growth, drought resisting, disease-resistant while the soil containing heavy metal and organic contamination can be repaired
Earth, the service efficiency for improving fertilizer;In terms of medical treatment:Medicinal fulvic acid has hemostasis, antineoplastic action, to burning and scalding
Position also has the effect of notable;In animal husbandry:Fulvic acid is added in the feed of nutrition purposes, especially for feeding animals, the growth of animal is sent out
Educate also has preventive and therapeutic effect with good facilitation while to the domestic animal and fowl's enteritis disease of animal.
Probiotics (Probiotic) word from Greek, is meant " managing life ".The concept of probiotics is earliest
Nineteen sixty-five is started from, LillyDM suggested that probiotics referred to balance microbial population of animal intestinal tract beneficial promotion substance or micro- first at that time
Biology.1989, probiotics was defined as by FullerR etc. again on experiment basis:" probiotics is that having for supplement nursing is lived
Property microorganism, and good health effect can be generated to host animal by improving the balance of intestinal flora "；It is the same year, beautiful
((FDA) and feed association (AAFCO) have issued can be with the safe bacterial strain 41 of Direct-fed animal for Food and Drug Administration of state
Kind, wherein 17 plants of lactic acid bacteria class, 6 plants of Bifidobacterium class, 5 plants of bud pole bacterium class, 4 plants of Bacteroideae, 2 plants of der Pilz (aspergillus niger,
Aspergillus oryzae), 1 plant of budding fungus, 6 plants of coccus and propionic acid mushroom.Thereafter with the emergence of probiotics relevant healthcare product, benefit
The definition of raw bacterium is also proposed and is corrected continuous.Until 2001, FAO (Food and Agriculture Organization of the United Nation) (FAO) and the World Health Organization
(WHO) probiotics is newly defined as by joint specialist group：Probiotics refers to after taking in right amount, is beneficial to its host health
Microorganism living.At present, this definition is known as the most suitable definition of probiotics by industry.Probiotics is widely present in nature,
It is mainly derived from human body and animal intestinal tract normal physiological bacterium and non-bowel bacterium.Lactic acid bacteria (Lacticacidbacteria)
Refer to fermenting carbohydrate, a kind of general name without bud armful, gram-positive bacterium of the primary product for lactic acid.Because it can be by carbon aquation
It closes object and is fermented into lactic acid, therefore gain the name.Lactic acid bacteria is one of physiology flora particularly important in people and animal intestinal tract.With society
Fast development, people's living standard are also subjected to be originated from pressure everywhere, the thing followed various diseases while raising so that
People increasingly pay attention to the health of itself.And probiotic lactobacillus is very useful to intestinal health, as increased lactic acid bacteria in enteron aisle
Amount reduces harmful bacteria, prevents diarrhea；It improves immunity of organisms, activated macrophage and generates tumor necrosis factor, interference
The antibody such as element and IgA；It adsorbs carcinogenic substance and reduces carcinogenic concentration in excrement, reduce and the activation relevant enzyme activity of carcinogenic substance
Property；Reduce serum cholesterol and blood pressure lowering etc..At present, probiotic lactic acid bacterium is mostly from the dairy products of fermentation, the meat products of fermentation
And traditional pickles screen.Screening lactobacillus has not been reported from fulvic acid solution.
Invention content
The present invention relates to a kind of lactic acid bacteria for being isolated from fulvic acid, it is an object of the present invention to provide with to acid with
Strong tolerance and the lactic acid bacteria of antibacterial activity.
To achieve these goals, the present invention provides a kind of lactic acid bacteria strains, which is isolated from fulvic acid solution.
The lactic acid bacteria strains of the present invention have following characteristics：1. morphology-on lactic acid bacteria MRS Agar Platings,
Morphology after being cultivated 48 hours in 37 DEG C：I. the shape of bacterium colony, size and color：Round, 2mm × 2mm, white, surface
It is smooth.Ii. Gram's staining：It is positive.Iii. type：Rod-type (bacillus).Iv. sporogenesis：Nothing.V. migration：Nothing.
2. physiological characteristics i. growth temperatures：25-45 DEG C of ii. grows pH：PH 4.0-10.0 iii. optimum growth temperatures：37-40
DEG C iv. the most suitable growthes pH：The influence of pH 6.0-8.0 3. oxygen：Amphimicrobian.4. the utilizability of carbohydrate：Glycerine-, core
Sugar-, adonitol-, galactolipin+, D-Glucose+, D-Fructose+, D-MANNOSE+, mannitol-, sorbierite-, N- Acetylglucos
Glycosides+, aesculin+, salicin+, cellobiose+, maltose+, lactose+, melibiose-, sucrose+, trehalose+, inulin-, pine three
Sugar-, gossypose-, starch-, β-gentiobiose-, D- turanoses+, the acid resistance of D-Tag+5.：In 2.0 times existence of pH.
6. for the antibacterial activity of pathogenic bacteria：For Escherichia coli, staphylococcus aureus, salmonella typhimurium, wax-like gemma bar
Bacterium, Listeria monocytogenes and proteus mirabilis have antibacterial activity.
The present invention also provides a kind of compositions of the lactic acid bacteria comprising effective dose.The composition of the present invention can be according to
Food, food additives, animal feed and animal feed additive form provide.New lactic acid bacteria provided by the present invention,
With excellent acid resistance antibacterial activity, become as the seed for producing various fermented dairy products and other fermented foods
Good candidate.Fermented dairy product as described herein can enumerate yogurt, cheese and butter, and other fermentations as described herein are eaten
Product can enumerate bean curd, soya sauce, jelly and pickles, however always not be limited to this.Bacterial strain is replaced with the lactic acid bacteria of the present invention, it is only logical
It crosses conventional method and is easy with production fermented dairy product and fermented food.
It can be according to the only composition comprising new lactic acid bacteria provided by the invention or containing any acceptable carrier
Edible form come produce the present invention lactobacillus food product.The lactic acid bacteria of the present invention can be added to not comprising any benefit
In the food of raw bacterium or in the food of included several probiotics.Next life lactic acid-producing bacteria can be shared with the lactic acid bacteria of the present invention to eat
The microorganism of product must adapted to human or animal's intake, and with probiotic active, such as pathogenic bacteria or improvement lactation is inhibited to move
Microbial balance in object enteron aisle, but always it is not limited to this.Probiotic micro-organisms can enumerate yeast, such as Blastocystis, false silk
Saccharomyces, pichia category (Pichia) and Torulopsis (Torulopsis)；Fungi, such as Eurotium, rhizopus
Category, Mucor and Penicillium；And bacterium, the bacterium belong to lactobacillus, Bifidobacterium, Leuconostoc, breast
Coccus, bacillus, streptococcus, Propionibacterium, enterococcus spp and Mycosphaerella.To more preferably have excellent benefit
The probiotic micro-organisms mixture of raw bacterium activity and immune-enhancing activity and active anticancer is added to the lactic acid bacteria food of the present invention
To obtain stronger effect in product.Be suitable for the invention the carrier of lactobacillus food for example have diluent, high microsteping additive,
Encapsulant and lipid.Capsule, culture suspension or dry powder can be made in the lactic acid bacteria of the present invention.It furthermore it is possible to will packet
Composition containing lactic acid bacteria of the present invention is made as animal feed or animal feed additive.The animal feed additive of the present invention can be made
Into dried forms or liquid form, other non-pathogenic microorganisms can be included.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, it is right With reference to embodiment
The present invention is described in further detail, but the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following implementations
Example.
Embodiment 1：The lactic acid bacteria of fulvic acid solution is isolated from by fulvic acid solution according to different dosage, is applied to breast
In sour bacterium Selective agar medium.By medium culture 1-2 days at 37 DEG C, then bacterium colony therein is picked by form and color
Choosing.The bacterium colony of separation is subjected to Gram's staining, then observes to select Gram-positive and there is rod-shaped knot under the microscope
The bacterium colony of structure.By the bacterium colony culture of selection 24 hours in MRS fluid nutrient mediums (pH 6.8) at 37 DEG C.PH is selected to be less than 4.5
Culture solution in bacterium colony.Bacterium colony culture, separation are shown into acid proof lactic acid bacteria strains in MRS culture mediums (pH 2.0),
And pass through microbial physiology biochemical test and 16S rRNA sequencing identified.
Embodiment 2：To the antibacterial activities of pathogenic bacteria at 37 DEG C, cultivated in beef extract-peptone fluid nutrient medium big
Enterobacteria, staphylococcus aureus, salmonella typhimurium, Bacillus cercus, Listeria monocytogenes and unusual
Then proteus 12 hours is inoculated into 6 with the concentration of 105 CFU/ml and is trained containing solid beef extract-peptone agar respectively
It supports on base tablet.The lactic acid bacteria newly screened with oese one ring of inoculation in each culture medium, cultivates 24 hours at 37 DEG C.
The result is that observing clear growth inhibition ring in lactic acid bacteria periphery of bacterial colonies, showing the bacterial strain of the present invention has various pathogenic bacteria
Antibacterial activity.
Claims (5)
1. a kind of lactic acid bacteria with probiotic active.
2. according to claim 1, it is characterised in that the probiotics separation screening is from fulvic acid.
3. a kind of composition, the composition includes the lactic acid bacteria described in claim 1 of effective dose.
4. composition according to claim 3, wherein the composition be selected from food, food additives, animal feed and
The group that animal feed additive is formed.
5. composition according to claim 4, wherein the animal feed additive is included selected from other non-micro- lifes of causing a disease
The group that object, enzyme and its mixture are formed it is one or more.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611135319.0A CN108220177A (en) | 2016-12-11 | 2016-12-11 | Being isolated from fulvic acid has the lactic acid bacteria of probiotic active |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611135319.0A CN108220177A (en) | 2016-12-11 | 2016-12-11 | Being isolated from fulvic acid has the lactic acid bacteria of probiotic active |
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| CN108220177A true CN108220177A (en) | 2018-06-29 |
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| CN201611135319.0A Pending CN108220177A (en) | 2016-12-11 | 2016-12-11 | Being isolated from fulvic acid has the lactic acid bacteria of probiotic active |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113812631A (en) * | 2020-06-18 | 2021-12-21 | 陈信行 | Humic acid probiotic compound and preparation process thereof |
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2016
- 2016-12-11 CN CN201611135319.0A patent/CN108220177A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113812631A (en) * | 2020-06-18 | 2021-12-21 | 陈信行 | Humic acid probiotic compound and preparation process thereof |
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Application publication date: 20180629 |