CN108217973A - A kind of efficient degradation method of sulphadiazine - Google Patents

A kind of efficient degradation method of sulphadiazine Download PDF

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CN108217973A
CN108217973A CN201810008548.9A CN201810008548A CN108217973A CN 108217973 A CN108217973 A CN 108217973A CN 201810008548 A CN201810008548 A CN 201810008548A CN 108217973 A CN108217973 A CN 108217973A
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sulphadiazine
phanerochaete chrysosporium
degradation
solution
days
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张婷
裘文慧
郑春苗
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Southwest University of Science and Technology
Southern University of Science and Technology
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/38—Organic compounds containing nitrogen
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/40—Organic compounds containing sulfur

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Abstract

The present invention relates to a kind of method of efficient degradation sulphadiazine, the method is that Phanerochaete chrysosporium is added in sulphadiazine solution to form cultivating system progress biodegradation.The present invention provides a kind of efficient, low cost and the degradation pathways of the sulphadiazine of safety.Compared with prior art, method of the invention have it is safe, secondary pollution will not be brought to water body etc., there is fast with degradation speed, used Phanerochaete chrysosporium at low cost to have many advantages, such as to be easy to get and be easily enlarged culture, it is cheap.Using the method for the present invention, it is 3 days most fast within degradation rate up to 100%, well solved the problem that sulfa antibiotics are degraded in the environment, there is generally applicable meaning.The method of the present invention is for sulphadiazine antibiotic of degrading present in environment; it is reduced in the environment such as the residual quantity in water body and soil, using the method processing soil or water body of the present invention, can be preserved the ecological environment; the generation of bacterial resistance is reduced, so as to protect human health.

Description

A kind of efficient degradation method of sulphadiazine
Technical field
The present invention relates to be difficult to the sulphadiazine degraded in a kind of degradation technique field of antibiotic more particularly to nature Efficient degradation method, belong to biotechnology.
Background technology
Sulphadiazine (English name sulfadiazine, abridge SDZ) is a kind of artificial synthesized, is widely used in facing Broad spectrum antibiotic in bed, animal husbandry and culture fishery.After such drug use largely in the form of prototype or metabolin Into environment, cause to detect its residual in natural water body and soil.This can not only destroy ecological environment, cause drug-fast bacteria It generates, can also health be jeopardized by food chain enrichment.Thus, sulfa antibiotics have curative effect it is good, Easy to use, the advantages that property is stable, cheap, irreplaceable role is played in the disease control of aquaculture.But It is, also due to it is widely used, sulfa antibiotics to be caused to do great damage sea-farming ecological environment.Therefore, it visits Rope it is a kind of can the method for efficient degradation sulphadiazine seem most important.
The method of current degradation sulphadiazine is including γ-radiation degradation, (removal rate highest only reaches ultrasonic wave removal method 83%), silicon dioxide nanosphere area load has catalytic degradation of the inorganic nano-particle of catalytic action as catalyst Method, the chemical-agent technique containing palladium ion, photocatalysis catalytic degradation method, cytochrome P 450 enzymes edman degradation Edman.Except these aforementioned skills Except art, about utilizing, the research report of biodegradable antibiotic is less, and sulfa antibiotics effectively remove both at home and abroad at present As problem urgently to be resolved hurrily.And or there is these existing methods radiativity or removal rate not to reach requirement or urge The preparation of agent or enzyme or extracting method are extremely complex, cost is quite high, more pollutants are generated in preparation process, lead to this The being greatly limited property of application of a little methods.
Phanerochaete chrysosporium is one kind of whiterot fungi, mainly by secreting non-specific extracellular lignin-degrading enzymes to mesh Mark product is degraded.Although some researches show that Phanerochaete chrysosporium has the aromatic compound of similar lignin structure Good degradation effect, including Chloroanilines, Polychlorinated biphenyls, polycyclic aromatic hydrocarbon and nitrobenzene etc., but not yet discovery will It is applied to degradation sulphadiazine and the relevant report of specific biodegrading process and operating condition.
Invention content
In order to solve the above problem of the prior art, the present invention provides a kind of method of efficient degradation sulphadiazine, described Method security, it is easy to operate, at low cost, can quickly and almost 100% ground high efficiency removal sulphadiazine method, suitable for pushing away Wide application is in the treatment of waste water.
In order to achieve the above object, the main technical schemes that the present invention uses include：A kind of efficient degradation sulphadiazine Method, the method are that Phanerochaete chrysosporium is added in sulphadiazine solution to form cultivating system progress biodegradation.
Preferably, the Phanerochaete chrysosporium size is the mycelium pellet of 3mm~10mm, and preferably size is the bacterium of 5mm Pompon.
Preferably, between the initial pH value of the cultivating system is 4.6~7, more preferably 4.79~6.8.
Preferably, the initial pH of the cultivating system is 5.79~5.82.
Preferably, the temperature environment of the cultivating system is 26 DEG C, 30 DEG C or 37 DEG C, more preferably 37 DEG C.
Preferably, added in the sulphadiazine solution per 10mg/L the additive amount of the Phanerochaete chrysosporium for 2g ± 10%.
Preferably, it further includes and the Phanerochaete chrysosporium metabolism liquid is added in into sulphadiazine solution.Preferably, it adds Measure the Phanerochaete chrysosporium metabolism liquid that 5mL ± 10% is added in for the sulphadiazine solution of every 10mg/L.
Preferably, the method further includes the incubation step of Phanerochaete chrysosporium：Phanerochaete chrysosporium is inoculated in Solid medium, 28 DEG C~32 DEG C are cultivated 2~5 days；Then a certain amount of spore is taken to be enlarged culture using liquid training method, 28 DEG C~ 32 DEG C are cultivated 4~7 days, obtain the Phanerochaete chrysosporium mycelium pellet of size 3mm~10mm and its metabolism liquid.
Wherein, the solid medium is potato dextrose agar, and the culture medium that the liquid training method uses is Minimal medium.
Preferably, the minimal medium is following component：
It is added in every 1L deionized waters：Glucose 10g, KH2PO42g ± 10%, MgSO4·7H2O 0.5g、CaCl2 0.1g、MnSO4 0.03g、NaCl 0.06g、FeSO4·7H2O 6mg、CoCl2 6mg、ZnSO4·7H2O 6mg、CuSO4 6mg、AlK(SO4)2·12H2O 0.6mg、H3BO3 0.6mg、Na2MoO4·2H2O 0.6mg, yeast leaching liquor 0.012g ± 10%th, ammonium tartrate 0.2g ± 10%, vitamin B1 1mg, veratryl alcohol 0.07g and Tween 80 emulsifier 0.5g, pH from So.
The present invention also provides a kind of methods of efficient degradation sulphadiazine, and described method includes following steps：
S1：Cultivate Phanerochaete chrysosporium：Phanerochaete chrysosporium is inoculated in solid medium, 28 DEG C~32 DEG C cultures 2~5 days；Then a certain amount of spore is taken to be enlarged culture using liquid training method, 28 DEG C~32 DEG C are cultivated 4~7 days, obtain size 3mm The Phanerochaete chrysosporium mycelium pellet of~10mm and its metabolism liquid；
S2：Degradation sulphadiazine：By addition ± 10% Phanerochaete chrysosporiums of 2g in the sulphadiazine solution of every 10mg/L Degradation culture solution is formed with the Phanerochaete chrysosporium metabolism liquid of 5mL ± 10%, keeps the temperature at 26 DEG C or 37 DEG C, initial pH Value is adjusted between 5.79~5.82, is cultivated 3~7 days.
Preferably, in the step S2, degradation culture solution initial pH value is adjusted to 5.79, and temperature is maintained at 37 DEG C, culture Time is 3 days.
The beneficial effects of the invention are as follows：
The present invention provides a kind of efficient, low cost and the degradation pathways of the sulphadiazine of safety.With γ-radiation degradation Compare, the present invention is safe；It significantly improves relative to supercritical ultrasonics technology degradation rate, relative to palladium-gold catalyst method, will not feed water Body brings secondary pollution, and relative to silicon dioxide nanosphere area load inorganic nano-particle catalyst method, the present invention has The effect of at low cost and degradation speed fast (removing up to 100% for most fast 3 days)；Compared with cytochrome P 450 enzymes method, institute of the present invention The Phanerochaete chrysosporium used has many advantages, such as to be easy to get and is easily enlarged culture, cheap.The present invention utilizes yellow archespore hair The method of flat lead fungi degradation sulphadiazine, has well solved the problem that sulfa antibiotics are degraded in the environment, has extensive Applicable meaning.
The method of the present invention for sulphadiazine antibiotic of degrading present in environment, reduce its in the environment such as water body and Residual quantity in soil using the method processing soil or water body of the present invention, can preserve the ecological environment, reduce bacterial resistance Generation, so as to protect human health.
Description of the drawings
Fig. 1 shows influences of the initial pH during degradation sulphadiazine of the embodiment of the present invention 1 to sulphadiazine degradation rate.
Influence of the temperature environment to sulphadiazine degradation rate during Fig. 2 expression degradation sulphadiazines of the embodiment of the present invention 2.
Specific embodiment
In order to preferably explain the present invention, in order to understand, below in conjunction with the accompanying drawings, by specific embodiment, to this hair It is bright to be described in detail.
Technical solution provided by the present invention includes：
A kind of method of efficient degradation sulphadiazine, the method are that Phanerochaete chrysosporium is added to sulphadiazine is molten Cultivating system is formed in liquid and carries out biodegradation.
Preferably, the Phanerochaete chrysosporium size is the mycelium pellet of 3mm~10mm, and preferably size is the bacterium of 5mm Pompon.
Preferably, between the initial pH value of the cultivating system is 4.6~7, preferably 4.79~6.8.
Preferably, the initial pH of the cultivating system is 5.79~5.82.
Preferably, the temperature environment of the cultivating system is 26 DEG C or 37 DEG C, preferably 37 DEG C.
Preferably, added in the sulphadiazine solution per 10mg/L the additive amount of the Phanerochaete chrysosporium for 2g ± 10%.
Preferably, it further includes and the Phanerochaete chrysosporium metabolism liquid is added in into sulphadiazine solution.Preferably, it adds Measure the Phanerochaete chrysosporium metabolism liquid that 5mL ± 10% is added in for the sulphadiazine solution of every 10mg/L.
Preferably, the method further includes the incubation step of Phanerochaete chrysosporium：Phanerochaete chrysosporium is inoculated in Solid medium, 28 DEG C~32 DEG C are cultivated 2~5 days；Then a certain amount of spore is taken to be enlarged culture using liquid training method, 28 DEG C~ 32 DEG C are cultivated 4~7 days, obtain the Phanerochaete chrysosporium mycelium pellet of size 3mm~10mm and its metabolism liquid.
The present invention also provides another technical solutions：
A kind of method of efficient degradation sulphadiazine, described method includes following steps：
S1：Cultivate Phanerochaete chrysosporium：Phanerochaete chrysosporium is inoculated in solid medium, 28 DEG C~32 DEG C cultures 2~5 days；Then a certain amount of spore is taken to be enlarged culture using liquid training method, 28 DEG C~32 DEG C are cultivated 4~7 days, obtain size 3mm The Phanerochaete chrysosporium mycelium pellet of~10mm and its metabolism liquid；
S2：Degradation sulphadiazine：By addition ± 10% Phanerochaete chrysosporiums of 2g in the sulphadiazine solution of every 10mg/L Liquid is metabolized with the Phanerochaete chrysosporium of 5mL ± 10%, keeps the temperature at 26 DEG C or 37 DEG C, initial pH value adjusted to 5.79~ Between 5.82, cultivate 3~7 days.
Preferably, in the step S2, initial pH value is adjusted to 5.79, and temperature is maintained at 37 DEG C, and incubation time is 3 days, The degradation rate of sulphadiazine can reach 100% in solution.
Below in conjunction with specific experiment, technical scheme of the present invention and technique effect are described in detail.
Experimental strain, reagent and instrument
Phanerochaete chrysosporium (being purchased from Guangdong Province's Culture Collection), sulphadiazine (SDZ, 99%), Ma Ling Potato glucose agar medium (PDA culture medium), minimal medium are (in 1L deionized waters:Glucose 10g；KH2PO42g； MgSO4·7H2O 0.5g；CaCl20.1g；MnSO40.03g；NaCl 0.06g；FeSO4·7H2O 6mg；CoCl26mg； ZnSO4·7H2O 6mg；CuSO46mg；AlK(SO4)2·12H2O 0.6mg；H3BO30.6mg；Na2MoO4·2H2O 0.6mg； Yeast leaching liquor 0.012g；Ammonium tartrate 0.2g；Vitamin B11 mg；Veratryl alcohol 0.07g；Tween 80 emulsifier 0.5g, pH It is natural).
Constant temperature oscillator, Agilent high performance liquid chromatograph.
Embodiment one
The experimental procedure and test condition of embodiment one are as follows：
(11) Spawn incubation
Phanerochaete chrysosporium is inoculated in PDA culture medium, 30 DEG C are cultivated 3 days；Then a certain amount of yellow archespore hair is taken Flat lead fungi spore is enlarged culture using fluid nutrient medium, and 30 DEG C are cultivated 5~7 days, obtain size averagely about 3~10mm's or so Phanerochaete chrysosporium mycelium pellet and its metabolism liquid.
(12) culture of degradation sulphadiazine
Using methanol and dimethyl sulfoxide (DMSO) 1:1 Compound mixed solution sulphadiazine storing solution【Due to high concentration sulphadiazine Solubility in water is poor, therefore uses methanol and dimethyl sulfoxide (DMSO) 1:1 Compound mixed solution sulphadiazine storing solution.Due to During high-dissolvability using the method for the present invention there is very high degradation, also can prove that the method for the present invention to low concentration in water Sulphadiazine equally has more significant degradation effect】, make a concentration of 10mg/mL of sulphadiazine.Seven are set in the present embodiment Experimental group using 7 50mL conical flasks, and is respectively designated as E1, E2, E3, E4, E5, E6, E7.The operation of each experimental group is such as Under：
E3 groups：Take the Phanerochaete chrysosporium mycelium pellet about 2g obtained in (11) and metabolism liquid 5mL and 15ml inorganic salts training In nutrient solution to the conical flask of 50mL, the sulphadiazine 20ul of 10mg/ml is added so that sulphadiazine is a concentration of in conical flask 10mg/L measures the natural pH about 4.79 of culture solution in conical flask, then adjusts culture solution with the HCl/water solution of 1mol/L at this time PH value makes it be 2.94.
E4 groups:According to the identical operations of E3, the difference lies in finally using HCL adjust culture solution pH be adjusted to 3.74。
E5 groups：According to the identical operations of E3, the difference lies in finally not in cone governor shape bottle culture solution pH, It is allowed to keep nature pH about 4.79 (natural ph of culture medium).
E6 groups:According to the identical operations of E3, the difference lies in finally adjust culture solution pH using NaOH to adjust It is 5.82.
E7 groups:According to the identical operations of E3, the difference lies in finally adjust culture solution pH using NaOH to adjust It is 6.73.
E1 is blank control group：In the 50mL conical flasks of blank add in 10mg/ml sulphadiazine 20ul, addition go from Sub- water adjusts a concentration of 10mg/L of sulphadiazine in conical flask.Keep nature pH about 4.79.E1 groups are not added with mycelium pellet and metabolism Liquid, but the subsequently culture in a manner of identical with E3-E7, it is therefore an objective to observe the natural decomposition of SDZ.
E2 is high-temperature inactivation control group：According to the identical operations of E3, keep nature pH about 4.79.Difference exists In, with high-pressure sterilizing pot sterilize 30min, at this time Phanerochaete chrysosporium mycelium pellet be inactivated；But subsequently with identical with E3-E7 Mode culture, it is therefore an objective to observe suction-operated of the mycelium pellet to SDZ.
After the completion of more than each group culture solution is prepared, cultivate in an identical manner：After all sealing taper bottleneck with sterile film It is 30 DEG C, constant temperature oscillator rotating speed 150rmp in constant incubator temperature, cultivates 6 days.Every group of experiment repeats there are three being all provided with, often It measures the concentration of remnants SDZ in culture medium, calculates degradation rate.
(13) degradation rate test method
It is taken out from each conical flask and supernatant is taken after 1mL culture solutions centrifuge in centrifuge tube, and filtered with the water system of 0.22um Membrane filtration.Using the concentration of the SDZ in high effective liquid chromatography for measuring culture solution.Chromatographic column selects Agilent C18 reversed-phase columns (4mm*10cm, 3.5um), the phosphoric acid and acetonitrile ratio that mobile phase is 0.02mol/l are 80：20 mixed liquor, UV detector, Test wavelength is 270nm, and the retention time of SDZ is 1.937min.
Experimental result is as shown in Fig. 1 or following table：
It can be seen that blank control group E1, high-temperature inactivation control group E2, pH are respectively 2.94 and 3.74 4 groups of experiments 6 Degradation rate stabilization in it is within 20%.During pH=5.82, the 3rd day degradation rate reach more than 90%, pH=4.79 and Degradation rate when 6 days of two groups of 5.82 reaches 100%.It can be seen that at 30 DEG C, pH=5.82 is optimal initial pH.But under 30 DEG C of culture environments, initial pH6.73 and pH4.79 are similarly respectively provided with comparatively ideal degradation effect.
Embodiment two
The experimental procedure and test condition of embodiment two are as follows：
(11) Spawn incubation
Phanerochaete chrysosporium is inoculated in PDA culture medium, 30 DEG C are cultivated 3 days；Then a certain amount of yellow archespore hair is taken Flat lead fungi spore is enlarged culture using fluid nutrient medium, and 30 DEG C are cultivated 5 days, obtain the size averagely yellow spore of about 4~6mm or so The flat lead fungi mycelium pellet of raw wool and its metabolism liquid.
(12) culture of degradation sulphadiazine
Using methanol and dimethyl sulfoxide (DMSO) 1:1 Compound mixed solution sulphadiazine storing solution, makes sulphadiazine a concentration of 10mg/mL.In the present embodiment set five experimental groups, using 5 50mL conical flasks, and be respectively designated as X1, X2, X3, X4, X5.The operation of each experimental group is as follows：
X3, X4, X5 experimental group：The Phanerochaete chrysosporium mycelium pellet about 2g obtained in (11) and metabolism liquid 5mL are taken respectively In the conical flask of 15ml inorganic salts culture solution to 50mL, the sulphadiazine 20ul of 10mg/ml is added so that sulphur in conical flask A concentration of 10mg/L of amic metadiazine measures the natural pH about 4.79 of culture solution in conical flask, then the NaOH water with 1mol/L at this time The pH value that solution adjusts culture solution makes it be 5.79.
X1 is blank control group：In the 50mL conical flasks of blank add in 10mg/ml sulphadiazine 20ul, addition go from Sub- water adjusts a concentration of 10mg/L of sulphadiazine in conical flask, and the pH value of culture solution is adjusted with the NaOH aqueous solutions of 1mol/L and is made It is 5.79.X1 groups are not added with mycelium pellet and metabolism liquid, it is therefore an objective to observe SDZ natural decompositions.
X2 is high-temperature inactivation control group：According to the identical operations of X3, the difference lies in gone out with high-pressure sterilizing pot Bacterium 30min, at this time Phanerochaete chrysosporium mycelium pellet be inactivated, initial pH value 5.79.Purpose is the suction for observing mycelium pellet to SDZ Attached effect.
Condition of culture：
X3, X4, X5 tissue culture nutrient solution, which are prepared, to be completed, and seals taper bottleneck with sterile film, and respectively in constant temperature incubation box temperature Degree is set as under the conditions of 26 DEG C, 30 DEG C and 37 DEG C, constant temperature oscillator rotating speed 150rmp, is cultivated 6 days.X1, X2 tissue culture nutrient solution are prepared It completes, is 30 DEG C, constant temperature oscillator rotating speed 150rmp in constant incubator temperature after sealing taper bottleneck with sterile film, culture 6 My god.
Every group of experiment is all provided with there are three repetition, measures the concentration of remnants SDZ in culture medium, calculating degradation rate daily.
(13) degradation rate test method
It is taken out from each conical flask and supernatant is taken after 1mL culture solutions centrifuge in centrifuge tube, and filtered with the water system of 0.22um Membrane filtration.Using the concentration of the SDZ in high effective liquid chromatography for measuring culture solution.Chromatographic column selects Agilent C18 reversed-phase columns (4mm*10cm, 3.5um), the phosphoric acid and acetonitrile ratio that mobile phase is 0.02mol/l are 80：20 mixed liquor, UV detector, Test wavelength is 270nm, and the retention time of SDZ is 1.937min.
Experimental result is as shown in Fig. 2 or following table：
It can be seen that in above-mentioned experiment, the blank control of X1 groups and X2 groups high-temperature inactivation are equal to the degradation rate for impinging upon 6 days Within 10%, therefore the suction-operated of SDZ can be neglected in the natural degradation rate of SDZ and mycelium pellet.By comparing above It can find, in pH5.79, under the condition of culture that 26 DEG C, 30 DEG C and 37 DEG C of temperature, after the incubation time of 6 days, sulfanilamide (SN) is phonetic The degradation rate degradation rate of pyridine is all close to 100%.And when cultivating 2 days, two groups of experiments during 26 DEG C and 37 DEG C of temperature, sulphadiazine Degradation rate is above 80%.By comparing daily degradation rate and degradation speed, it is seen that 37 DEG C of degradations most beneficial for SDZ.But 26 DEG C, 30 DEG C and 37 DEG C are also all ideal temperature.
It is drawn a conclusion by embodiment 1-2, Phanerochaete chrysosporium and its metabolism liquid is added in sulphadiazine solution, When being co-cultured, condition of culture is initial pH=5.79~5.82, temperature is 37 DEG C, Phanerochaete chrysosporium bacterium ball size exists 3~10mm or so and when adding in a certain amount of metabolism liquid, the degradation rate highest of SDZ and degradation speed is most fast in culture solution, at 3 days Within degradation rate i.e. up to 100%.Therefore, Phanerochaete chrysosporium has obviously degradation to sulphadiazine, Degradation speed is quick and very efficient, suitable for industry, the fishery to a large amount of antibiotic containing sulphadiazine containing sulphadiazine Or agricultural effluent and soil carry out harmless treatment.

Claims (10)

  1. A kind of 1. method of efficient degradation sulphadiazine, which is characterized in that the method is to be added to Phanerochaete chrysosporium Cultivating system is formed in sulphadiazine solution and carries out biodegradation.
  2. 2. according to the method described in claim 1, it is characterized in that, the Phanerochaete chrysosporium size is 3mm~10mm's Mycelium pellet.
  3. 3. according to the method described in claim 1, it is characterized in that, the initial pH value of the cultivating system is 4.79~6.8.
  4. 4. according to the method described in claim 3, it is characterized in that, the initial pH of the cultivating system is 5.79~5.82.
  5. 5. according to the method described in claim 3, it is characterized in that, the temperature environment of the cultivating system for 26 DEG C, 30 DEG C or 37℃。
  6. 6. according to claim 1-5 any one of them methods, which is characterized in that added in the sulphadiazine solution per 10mg/L The additive amount of the Phanerochaete chrysosporium is 2g ± 10%.
  7. 7. the method according to right wants 6, which is characterized in that further include and the yellow archespore hair is added in into sulphadiazine solution Flat lead fungi is metabolized liquid, and the Phanerochaete chrysosporium that 5mL ± 10% is added in by the sulphadiazine solution of every 10mg/L is metabolized liquid.
  8. 8. the method according to right wants 1, which is characterized in that the method further includes the culture step of Phanerochaete chrysosporium Suddenly：Phanerochaete chrysosporium is inoculated in solid medium, 28 DEG C~32 DEG C are cultivated 2~5 days；Then a certain amount of spore is taken to use Liquid training method is enlarged culture, and 28 DEG C~32 DEG C are cultivated 4~7 days, obtain the Phanerochaete chrysosporium mycelium pellet of size 3mm~10mm And its metabolism liquid.
  9. A kind of 9. method of efficient degradation sulphadiazine, which is characterized in that described method includes following steps：
    S1：Cultivate Phanerochaete chrysosporium：Phanerochaete chrysosporium is inoculated in solid medium, 28 DEG C~32 DEG C cultures 2~5 My god；Then a certain amount of spore is taken to be enlarged culture using liquid training method, 28 DEG C~32 DEG C cultures 4~7 days, obtain size 3mm~ The Phanerochaete chrysosporium mycelium pellet of 10mm and its metabolism liquid；
    S2：Degradation sulphadiazine：By addition ± 10% Phanerochaete chrysosporiums of 2g and 5mL in the sulphadiazine solution of every 10mg/L ± 10% Phanerochaete chrysosporium metabolism liquid forms degradation culture solution, keeps the temperature at 26 DEG C or 37 DEG C, initial pH value tune It saves between 5.79~5.82, cultivates 3~7 days.
  10. 10. the according to the method described in claim 9, it is characterized in that, initial pH value tune for culture solution of degrading in the step S2 It is 5.79 to save, and temperature is maintained at 37 DEG C, and incubation time is 3 days.
CN201810008548.9A 2018-01-04 2018-01-04 A kind of efficient degradation method of sulphadiazine Pending CN108217973A (en)

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Application publication date: 20180629