CN108212097A - For the preparation method of the amino-acid modified organic lithium soapstone of bilirubin adsorption - Google Patents
For the preparation method of the amino-acid modified organic lithium soapstone of bilirubin adsorption Download PDFInfo
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Abstract
The invention discloses a kind of preparation methods of the amino-acid modified organic lithium soapstone for bilirubin adsorption, include the following steps：1) modifier solution, hectorite dispersion liquid are prepared；Under the conditions of 70~80 DEG C, modifier solution with hectorite dispersion liquid is uniformly mixed, is reacted, until reaction terminates, obtains dispersion liquid I；2) compound concentration is the amino acid solution of 0.1~0.5mol/L；Amino acid solution is uniformly mixed with dispersion liquid I, in pH value under the conditions of 4.5~6.5,70~80 DEG C, reaction until reaction terminates, through filtering, washing, drying, obtains the amino-acid modified organic lithium soapstone for bilirubin adsorption.Amino-acid modified organic lithium soapstone nanocomposite is up to 98% to the removal rate of bilirubin solution made from the preparation method.
Description
Technical field
The invention belongs to bioengineering separation fields, specially a kind of amino-acid modified organolithium for bilirubin adsorption
The preparation method of saponite.
Background technology
Hectorite (Hectorite, Hec) for it is artificial synthesized containing lithium, magnesium, silicon discoid hectorite class clay
Grain is made of one layer of magnesia octahedron of folder among upper and lower two layers of oxygen-octahedron；In octahedron part magnesium ion by monovalence lithium from
Son displacement, part hydroxyl ion are replaced by fluorine ion, form strong electron deficient and strong electronegativity structure.Because it is with excellent increasing
The performances such as consistence, dispersibility, adsorptivity and be widely used in the industries such as cosmetics, daily-use chemical industry, toothpaste.
It is handed over to increase the compatibility of hectorite and bioactive molecule, porosity, specific surface area, hydrophily, cation
The properties such as type are changed, can be improved by surface modification, common method has heat treatment and chemical treatment.Hectorite surface contains
There are abundant hydroxy groups, hectorite is modified using quaternary ammonium salt and silane coupling agent, hectorite dispersion is apparent after modification
Viscosity and surface elecrtonegativity effectively reduce, and hydrophobicity dramatically increases, and not only increase the stability of material and reinforcement after modifying
Absorption of the material to Organic substance in water.
Electrostatic phase interaction can occur with amido containing tetrapyrrole dicarboxylic acids, wherein carboxyl in the structure of bilirubin molecule
With carboxyl can form hydrogen bond with oxygen-containing or nitrogen group, and with hydrophobic structure phenyl ring, Long carbon chain hydrophobic phase can occur for pyrrolylmethyl
By hydrophobic, electrostatic force or interaction of hydrogen bond to bilirubin suction-operated can occur for interaction, therefore, sorbing material.Have
Machine hectorite can pass through electrostatic force adsorbing bilirubin；After amino acid modification, the amido on amino acid molecular can be with courage
Chemical action occurs for the carboxyl in red pigment molecule, and then enhances the specific adsorption to bilirubin molecule.CN102049242A is public
A kind of anion resin for bilirubin absorption and preparation method thereof is opened, this method process is complicated, and needs to disappear in reaction process
Consume a large amount of organic solvent.
Invention content
In order to solve the above technical problem, the present invention provides a kind of amino-acid modified organic lithium soaps for bilirubin adsorption
The preparation method of stone, amino-acid modified organic lithium soapstone nanocomposite obtained are up to the removal rate of bilirubin solution
98%.
For this purpose, technical scheme is as follows：
A kind of preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption, includes the following steps：
1) modifying agent is dissolved in distilled water, prepares modifier solution；
It is 1~5 that the modifying agent, which is quaternary ammonium salt or is mass ratio,：1 quaternary ammonium salt and the mixture of coupling agent；
Prepare hectorite dispersion liquid；
Under the conditions of 70~80 DEG C, the modifier solution with hectorite dispersion liquid is uniformly mixed, is reacted, until reaction
Terminate, obtain dispersion liquid I；
2) amino acid is dissolved in distilled water, compound concentration is the amino acid solution of 0.1~0.5mol/L；
The amino acid solution with the dispersion liquid I is uniformly mixed, is 4.5~6.5,70~80 DEG C of conditions in pH value
Under, reaction until reaction terminates, through filtering, washing, drying, obtains described for the amino-acid modified organic of bilirubin adsorption
Hectorite.
Further, the quaternary ammonium salt is cetyl trimethylammonium bromide, tetradecyltrimethylammonium bromide or dodecane
Base trimethylammonium bromide；
Further, the coupling agent is gamma-aminopropyl-triethoxy-silane, γ-(2,3- the third oxygen of epoxy), propyl trimethoxy
Base silane or γ-methacryloxy propylsilane etc..
Further, when the modifier solution is reacted with hectorite dispersion liquid, the mass ratio of hectorite and modifying agent for 1~
3：1.
Further, amino acid described in step 2) is lysine, arginine, serine, glycine, valine, phenyl third
Any one in propylhomoserin, aspartic acid and glutamic acid.
Further, the preparation method of hectorite dispersion liquid is in step 1)：Hectorite is scattered in steaming under agitation
In distilled water, its pH value 7~8 is adjusted, 1~3h is stirred under the conditions of 70~80 DEG C.
Further, the reaction time of modifier solution and hectorite dispersion liquid is 4~8h in step 1).
Further, the reaction time is 4~12h in step 2).
The present invention carries out solution intercalation with organic matter using hectorite as base material, by hectorite after swelling and reacts, and obtains organic
Hectorite.Amino acid is inserted not only through by interlayer by special adsorption again, while is also adsorbed in the surface of solids.Preparing one kind has
The novel bilirubin adsorption material of high specific, high-adsorption-capacity.This method is simple, and reaction condition is mild, easily controllable, cost
It is cheap.Due to the introducing of quaternary ammonium salt, make sorbing material obtained that there is certain anti-microbial property simultaneously.Lithium soap after amino-acid modified
Stone adsorbing bilirubin rate significantly improves, and shortens time of equilibrium adsorption.
Description of the drawings
Fig. 1 is the infrared spectrum of the primary modified hectorite that embodiment 1,2 obtains and amino-acid modified organic lithium soapstone；
Wherein, the infrared spectrum of primary modified hectorite that a-- embodiments 1 obtain；
The infrared spectrum of amino-acid modified organic lithium soapstone that b-- embodiments 1 obtain；What c-- embodiments 2 obtained once changes
The infrared spectrum of property hectorite；
The infrared spectrum of amino-acid modified organic lithium soapstone that d-- embodiments 2 obtain.
Fig. 2 is the scanning electron microscopic picture of primary modified hectorite that embodiment 1 obtains.
The primary modified hectorite and amino-acid modified organic lithium soapstone that Fig. 3 is to bilirubin solution adsorbance at any time
Between variation diagram；A is that the primary modified hectorite that embodiment 2 obtains changes over time figure to bilirubin solution adsorbance in figure；B is
The amino-acid modified hectorite that embodiment 2 obtains changes over time figure to bilirubin solution adsorbance.
Specific embodiment
Technical scheme of the present invention is described in detail below in conjunction with drawings and examples.
It is red that courage has been carried out to once modification hectorite or amino-acid modified organic lithium soapstone that section Example obtains below
Plain adsorption experiment, detailed process are as follows：
1. bilirubin buffer preparation：
Under the conditions of being protected from light, 0.02g bilirubin is accurately weighed in volumetric flask, moves into the NaOH solution of 0.1mol/L
10mL is gently shaken to being completely dissolved, and solution clear is in claret；Then pH=7.4 phosphate buffer solution 40mL are added in, gently
Shake uniform and static 10min；It is instant to match finally with deionized water polishing 100mL.Bilirubin pH value of buffer solution is 10~11
Between.
2. simulate the bilirubin adsorption experiment in serum：It weighs about 50mg and is once modified hectorite or amino-acid modified organic
Hectorite immerses the bilirubin buffer solution of 10mL Fresh, with 100rmin-1Rotating speed is in 37 DEG C of constant temperature oscillations.Take out warp
Bilirubin buffer solution after primary modified hectorite or the absorption of amino-acid modified organic lithium soapstone, measures under 438nm wavelength.
Note：Bilirubin solution adsorption experiment is completed under the conditions of being protected from light.
Embodiment 1
A kind of preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption, includes the following steps：
1) the once preparation of modified hectorite
8g hectorites are scattered under agitation in 350mL distilled water, jitter time 1h obtains transparent hectorite
Dispersion liquid.
4g cetyl trimethylammonium bromides are dissolved in 50mL distilled water and obtain modifier solution；
PH value=7 of hectorite dispersion liquid are adjusted, heating water bath adds in hectorite dispersion to 80 DEG C, by modifier aqueous solution
Liquid is stirred to react 4h under 80 DEG C of waters bath with thermostatic control, obtains dispersion liquid I；
2) preparation of amino-acid modified hectorite
4g lysines are dissolved in 50mL distilled water and obtain amino acid solution；
Amino acid solution is mixed into dispersion liquid I, with salt acid for adjusting pH value=5 of 0.1mol/L, in 80 DEG C of waters bath with thermostatic control
Under be stirred to react 4h, by reaction product through filter, and with 3000mL distill water washing, by filtration cakes torrefaction to get for bilirubin
The amino-acid modified organic lithium soapstone of absorption.
In bilirubin adsorption experiment, time of constant temperature oscillation is 6h (i.e. adsorption time), amino-acid modified organic lithium soapstone
Adsorption rate to bilirubin solution is 98.5%.
The dispersion liquid I that step 1) is obtained is filtered, and distills water washing with 3000mL, then by filtration cakes torrefaction, is obtained primary
Modified hectorite.
Hectorite will be once modified and carry out bilirubin adsorption experiment, isothermal vibration 6h (i.e. adsorption time) is once modified lithium
Saponite is 98.0% to the adsorption rate of bilirubin solution.
As it can be seen that amino-acid modified organic lithium soapstone improves the adsorption rate of bilirubin solution than being once modified hectorite
0.5%.
By Fig. 1 curves a, b it is found that in 980cm-1Place is-NO key stretching vibration peaks, in 2900cm-1~3100cm-1Place is
Aliphatic-CH is bimodal, in 3150cm-1~3700cm-1There are broad peak, corresponding-NH ,-OH moiety combinations, carboxyl, amine, acid imide in place
A large amount of presence of group, it is meant that the successful modification of lysine.
The scanning electron microscopic picture of primary modified hectorite that Fig. 2 is obtained, because pure hectorite surface is smooth, and passes through
It is modified to cross quaternary ammonium salt, apparent intercalation configuration occurs in surface, and there is little particle on surface so that lamella is disorderly arranged, these are existing
Successful preparation as showing primary modified hectorite.
Embodiment 2
A kind of preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption, includes the following steps：
1) the once preparation of modified hectorite
4g hectorites are scattered under agitation in 350mL distilled water, jitter time 1h obtains transparent hectorite
Dispersion liquid.
4g cetyl trimethylammonium bromides and 4g gamma-aminopropyl-triethoxy-silanes are dissolved in 50mL distilled water and obtained
Modifier solution；
PH value=7 of hectorite dispersion liquid are adjusted, heating water bath adds in hectorite dispersion to 80 DEG C, by modifier aqueous solution
Liquid is stirred to react 4h under 80 DEG C of waters bath with thermostatic control, obtains dispersion liquid I；
2) preparation of amino-acid modified hectorite
4g lysines are dissolved in 50mL distilled water and obtain amino acid solution；
Amino acid solution is mixed into dispersion liquid I, with salt acid for adjusting pH value=5 of 0.1mol/L, in 80 DEG C of waters bath with thermostatic control
Under be stirred to react 4h, by reaction product through filter, and with 3000mL distill water washing, by filtration cakes torrefaction to get for bilirubin
The amino-acid modified organic lithium soapstone of absorption.
In bilirubin adsorption experiment, time of constant temperature oscillation is 6h (i.e. adsorption time), amino-acid modified organic lithium soapstone
Adsorption rate to bilirubin solution is 83.5%.
The dispersion liquid I that step 1) is obtained is filtered, and distills water washing with 3000mL, then by filtration cakes torrefaction, is obtained primary
Modified hectorite.
Hectorite will be once modified and carry out bilirubin adsorption experiment, isothermal vibration 6h (i.e. adsorption time) is once modified lithium
Saponite is 74.6% to the adsorption rate of bilirubin solution.
As it can be seen that amino-acid modified organic lithium soapstone improves the adsorption rate of bilirubin solution than being once modified hectorite
8.9%.
By Fig. 1 curves c, d it is found that in 980cm-1Place is-NO key stretching vibration peaks, in 2900cm-1~3100cm-1Place is
Aliphatic-CH is bimodal, in 3150cm-1~3700cm-1There are broad peak, corresponding-NH ,-OH moiety combinations, carboxyl, amine, acid imide in place
A large amount of presence of group, it is meant that the successful modification of lysine.
By once modified hectorite and amino-acid modified organic lithium soapstone bilirubin adsorption amount change over time curve in Fig. 3
It is found that the adsorbance of bilirubin and adsorption rate are significantly increased after amino-acid modified organic lithium soapstone.
Embodiment 3
A kind of preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption, includes the following steps：
1) the once preparation of modified hectorite
12g hectorites are scattered under agitation in 350mL distilled water, jitter time 1h obtains transparent lithium soap
Stone dispersion liquid.
4g cetyl trimethylammonium bromides are dissolved in 50mL distilled water and obtain modifier solution；
PH value=7 of hectorite dispersion liquid are adjusted, heating water bath adds in hectorite dispersion to 80 DEG C, by modifier aqueous solution
Liquid is stirred to react 4h under 80 DEG C of waters bath with thermostatic control, obtains dispersion liquid I；
2) preparation of amino-acid modified hectorite
4g arginine is dissolved in 50mL distilled water and obtains amino acid solution；
Amino acid solution is mixed into dispersion liquid I, with salt acid for adjusting pH value=5 of 0.1mol/L, in 80 DEG C of waters bath with thermostatic control
Under be stirred to react 4h, by reaction product through filter, and with 3000mL distill water washing, by filtration cakes torrefaction to get for bilirubin
The amino-acid modified organic lithium soapstone of absorption.
In bilirubin adsorption experiment, time of constant temperature oscillation is 6h (i.e. adsorption time), amino-acid modified organic lithium soapstone
Adsorption rate to bilirubin solution is 81.9%.
The dispersion liquid I that step 1) is obtained is filtered, and distills water washing with 3000mL, then by filtration cakes torrefaction, is obtained primary
Modified hectorite.
Hectorite will be once modified and carry out bilirubin adsorption experiment, isothermal vibration 6h (i.e. adsorption time) is once modified lithium
Saponite is 76.3% to the adsorption rate of bilirubin solution.
As it can be seen that amino-acid modified organic lithium soapstone improves the adsorption rate of bilirubin solution than being once modified hectorite
5.6%.
Embodiment 4
A kind of preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption, includes the following steps：
1) the once preparation of modified hectorite
12g hectorites are scattered under agitation in 350mL distilled water, jitter time 1h obtains transparent lithium soap
Stone dispersion liquid.
8g cetyl trimethylammonium bromides and 4g gamma-aminopropyl-triethoxy-silanes are dissolved in 50mL distilled water and obtained
Modifier solution；
PH value=7 of hectorite dispersion liquid are adjusted, heating water bath adds in hectorite dispersion to 80 DEG C, by modifier aqueous solution
Liquid is stirred to react 4h under 80 DEG C of waters bath with thermostatic control, obtains dispersion liquid I；
2) preparation of amino-acid modified hectorite
4g arginine is dissolved in 50mL distilled water and obtains amino acid solution；
Amino acid solution is mixed into dispersion liquid I, with salt acid for adjusting pH value=5 of 0.1mol/L, in 80 DEG C of waters bath with thermostatic control
Under be stirred to react 4h, by reaction product through filter, and with 3000mL distill water washing, by filtration cakes torrefaction to get for bilirubin
The amino-acid modified organic lithium soapstone of absorption.
In bilirubin adsorption experiment, time of constant temperature oscillation is 6h (i.e. adsorption time), amino-acid modified organic lithium soapstone
Adsorption rate to bilirubin solution is 86.5%.
The dispersion liquid I that step 1) is obtained is filtered, and distills water washing with 3000mL, then by filtration cakes torrefaction, is obtained primary
Modified hectorite.
Hectorite will be once modified and carry out bilirubin adsorption experiment, isothermal vibration 6h (i.e. adsorption time) is once modified lithium
Saponite is 78.6% to the adsorption rate of bilirubin solution.
As it can be seen that amino-acid modified organic lithium soapstone improves the adsorption rate of bilirubin solution than being once modified hectorite
7.9%.
Embodiment 5
A kind of preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption, includes the following steps：
1) the once preparation of modified hectorite
8g hectorites are scattered under agitation in 350mL distilled water, jitter time 1h obtains transparent hectorite
Dispersion liquid.
4g cetyl trimethylammonium bromides are dissolved in 50mL distilled water and obtain modifier solution；
PH value=7 of hectorite dispersion liquid are adjusted, heating water bath adds in hectorite dispersion to 75 DEG C, by modifier aqueous solution
Liquid is stirred to react 4h under 75 DEG C of waters bath with thermostatic control, obtains dispersion liquid I；
2) preparation of amino-acid modified hectorite
4g serines are dissolved in 50mL distilled water and obtain amino acid solution；
Amino acid solution is mixed into dispersion liquid I, with salt acid for adjusting pH value=5.5 of 0.1mol/L, in 75 DEG C of thermostatted waters
4h is stirred to react under bath, by reaction product through filtering, and water washing is distilled with 3000mL, by filtration cakes torrefaction to get red for courage
The amino-acid modified organic lithium soapstone of element absorption.
In bilirubin adsorption experiment, time of constant temperature oscillation is 6h (i.e. adsorption time), amino-acid modified organic lithium soapstone
Adsorption rate to bilirubin solution is 93.8%.
The dispersion liquid I that step 1) is obtained is filtered, and distills water washing with 3000mL, then by filtration cakes torrefaction, is obtained primary
Modified hectorite.
Hectorite will be once modified and carry out bilirubin adsorption experiment, isothermal vibration 6h (i.e. adsorption time) is once modified lithium
Saponite is 89.9% to the adsorption rate of bilirubin solution.
As it can be seen that amino-acid modified organic lithium soapstone improves the adsorption rate of bilirubin solution than being once modified hectorite
3.9%.
Embodiment 6
A kind of preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption, includes the following steps：
1) the once preparation of modified hectorite
4g hectorites are scattered under agitation in 350mL distilled water, jitter time 1h obtains transparent hectorite
Dispersion liquid.
4g cetyl trimethylammonium bromides and 4g gamma-aminopropyl-triethoxy-silanes are dissolved in 50mL distilled water and obtained
Modifier solution；
PH value=7 of hectorite dispersion liquid are adjusted, heating water bath adds in hectorite dispersion to 75 DEG C, by modifier aqueous solution
Liquid is stirred to react 4h under 75 DEG C of waters bath with thermostatic control, obtains dispersion liquid I；
2) preparation of amino-acid modified hectorite
4g serines are dissolved in 50mL distilled water and obtain amino acid solution；
Amino acid solution is mixed into dispersion liquid I, with salt acid for adjusting pH value=6.5 of 0.1mol/L, in 75 DEG C of thermostatted waters
4h is stirred to react under bath, by reaction product through filtering, and water washing is distilled with 3000mL, by filtration cakes torrefaction to get red for courage
The amino-acid modified organic lithium soapstone of element absorption.
In bilirubin adsorption experiment, time of constant temperature oscillation is 6h (i.e. adsorption time), amino-acid modified organic lithium soapstone
Adsorption rate to bilirubin solution is 82.6%.
The dispersion liquid I that step 1) is obtained is filtered, and distills water washing with 3000mL, then by filtration cakes torrefaction, is obtained primary
Modified hectorite.
Hectorite will be once modified and carry out bilirubin adsorption experiment, isothermal vibration 6h (i.e. adsorption time) is once modified lithium
Saponite is 75.7% to the adsorption rate of bilirubin solution.
As it can be seen that amino-acid modified organic lithium soapstone improves the adsorption rate of bilirubin solution than being once modified hectorite
6.9%.
Embodiment 7
A kind of preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption, includes the following steps：
1) the once preparation of modified hectorite
8g hectorites are scattered under agitation in 350mL distilled water, jitter time 1h obtains transparent hectorite
Dispersion liquid.
4g tetradecyltrimethylammonium bromide is dissolved in 50mL distilled water and obtains modifier solution；
PH value=7 of hectorite dispersion liquid are adjusted, heating water bath adds in hectorite dispersion to 80 DEG C, by modifier aqueous solution
Liquid is stirred to react 4h under 80 DEG C of waters bath with thermostatic control, obtains dispersion liquid I；
2) preparation of amino-acid modified hectorite
4g glycine is dissolved in 50mL distilled water and obtains amino acid solution；
Amino acid solution is mixed into dispersion liquid I, with salt acid for adjusting pH value=5 of 0.1mol/L, in 80 DEG C of waters bath with thermostatic control
Under be stirred to react 4h, by reaction product through filter, and with 3000mL distill water washing, by filtration cakes torrefaction to get for bilirubin
The amino-acid modified organic lithium soapstone of absorption.
In bilirubin adsorption experiment, time of constant temperature oscillation is 6h (i.e. adsorption time), amino-acid modified organic lithium soapstone
Adsorption rate to bilirubin solution is 91.8%.
The dispersion liquid I that step 1) is obtained is filtered, and distills water washing with 3000mL, then by filtration cakes torrefaction, is obtained primary
Modified hectorite.
Hectorite will be once modified and carry out bilirubin adsorption experiment, isothermal vibration 6h (i.e. adsorption time) is once modified lithium
Saponite is 88.7% to the adsorption rate of bilirubin solution.
As it can be seen that amino-acid modified organic lithium soapstone improves the adsorption rate of bilirubin solution than being once modified hectorite
3.1%.
Embodiment 8
A kind of preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption, includes the following steps：
1) the once preparation of modified hectorite
4g hectorites are scattered under agitation in 350mL distilled water, jitter time 1h obtains transparent hectorite
Dispersion liquid.
4g tetradecyltrimethylammonium bromide and 4g γ-(the third oxygen of 2,3- epoxies), propyl trimethoxy silicane are dissolved in
Modifier solution is obtained in 50mL distilled water；
PH value=7 of hectorite dispersion liquid are adjusted, heating water bath adds in hectorite dispersion to 80 DEG C, by modifier aqueous solution
Liquid is stirred to react 4h under 80 DEG C of waters bath with thermostatic control, obtains dispersion liquid I；
2) preparation of amino-acid modified hectorite
4g glycine is dissolved in 50mL distilled water and obtains amino acid solution；
Amino acid solution is mixed into dispersion liquid I, with salt acid for adjusting pH value=5 of 0.1mol/L, in 80 DEG C of waters bath with thermostatic control
Under be stirred to react 4h, by reaction product through filter, and with 3000mL distill water washing, by filtration cakes torrefaction to get for bilirubin
The amino-acid modified organic lithium soapstone of absorption.
In bilirubin adsorption experiment, time of constant temperature oscillation is 6h (i.e. adsorption time), amino-acid modified organic lithium soapstone
Adsorption rate to bilirubin solution is 84.9%.
The dispersion liquid I that step 1) is obtained is filtered, and distills water washing with 3000mL, then by filtration cakes torrefaction, is obtained primary
Modified hectorite.
Hectorite will be once modified and carry out bilirubin adsorption experiment, isothermal vibration 6h (i.e. adsorption time) is once modified lithium
Saponite is 76.3% to the adsorption rate of bilirubin solution.
As it can be seen that amino-acid modified organic lithium soapstone improves the adsorption rate of bilirubin solution than being once modified hectorite
8.6%.
Claims (8)
1. the preparation method of a kind of amino-acid modified organic lithium soapstone for bilirubin adsorption, it is characterised in that including walking as follows
Suddenly：
1) modifying agent is dissolved in distilled water, prepares modifier solution；
It is 1~5 that the modifying agent, which is quaternary ammonium salt or is mass ratio,：1 quaternary ammonium salt and the mixture of coupling agent；
Prepare hectorite dispersion liquid；
Under the conditions of 70~80 DEG C, the modifier solution with hectorite dispersion liquid is uniformly mixed, is reacted, until reaction knot
Beam obtains dispersion liquid I；
2) amino acid is dissolved in distilled water, compound concentration is the amino acid solution of 0.1~0.5mol/L；
The amino acid solution is uniformly mixed with the dispersion liquid I, under the conditions of pH value is 4.5~6.5,70~80 DEG C,
Reaction until reaction terminates, through filtering, washing, drying, obtains the amino-acid modified organic lithium soap for being used for bilirubin adsorption
Stone.
2. preparation method as described in claim 1, it is characterised in that：The quaternary ammonium salt is cetyl trimethylammonium bromide, ten
Tetraalkyl trimethylammonium bromide or dodecyl trimethyl ammonium bromide.
3. preparation method as described in claim 1, it is characterised in that：The coupling agent for gamma-aminopropyl-triethoxy-silane,
γ-(the third oxygen of 2,3- epoxies), propyl trimethoxy silicane or γ-methacryloxy propylsilane etc..
4. preparation method as described in claim 1, it is characterised in that：When the modifier solution is reacted with hectorite dispersion liquid,
The mass ratio of hectorite and modifying agent is 1~3：1.
5. preparation method as described in claim 1, it is characterised in that：Amino acid described in step 2) is lysine, arginine, silk
Any one in propylhomoserin, glycine, valine, phenylalanine, aspartic acid and glutamic acid.
6. preparation method as described in claim 1, it is characterised in that：The preparation method of hectorite dispersion liquid is in step 1)：It will
Hectorite is scattered in distilled water under agitation, adjusts its pH value 7~8, and 1~3h is stirred under the conditions of 70~80 DEG C.
7. preparation method as described in claim 1, it is characterised in that：Modifier solution and hectorite dispersion liquid is anti-in step 1)
It is 4~8h between seasonable.
8. preparation method as described in claim 1, it is characterised in that：The reaction time is 4~12h in step 2).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810072982.3A CN108212097B (en) | 2018-01-25 | 2018-01-25 | The preparation method of amino-acid modified organic lithium soapstone for bilirubin adsorption |
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