CN103585638A - Preparation method for sodium alginate and calcium carbonate hybrid particles - Google Patents
Preparation method for sodium alginate and calcium carbonate hybrid particles Download PDFInfo
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- CN103585638A CN103585638A CN201310590485.XA CN201310590485A CN103585638A CN 103585638 A CN103585638 A CN 103585638A CN 201310590485 A CN201310590485 A CN 201310590485A CN 103585638 A CN103585638 A CN 103585638A
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Abstract
The invention relates to a preparation method for sodium alginate and calcium carbonate hybrid particles. The preparation method includes the following steps: dissolving sodium alginate in a solvent at a temperature ranging from 70 DEG C to 80 DEG C to obtain a sodium alginate solution; preparing a sodium carbonate solution and a calcium chloride solution at the indoor temperature; adding the sodium alginate solution into the sodium carbonate solution and stirring the mixture to obtain a mixed liquor; quickly adding the calcium chloride solution into the mixed liquor, and carrying out stirring, centrifugation and drying to obtain the hybrid particles. The preparation method has the advantages that the applied raw materials are cheap and easy to obtain, and the hybrid particles have high biodegradability and biocompatibility, as well as potentials for application and subsequent relevant experimental analysis.
Description
Technical field
The invention belongs to the preparation field of hydridization granular materials, particularly a kind of preparation method of sodium alginate-calcium carbonate hybrid granule.
Background technology
Biodegradable high-molecular refers to by the chemical action of the microorganism of nature or interpolation, and polymer substance is resolved into little minute compound, then enters natural cyclic process, and this method is succinctly effective, and the protection of environment is had to positive effect.Meanwhile, along with the development of new and high technology, biodegradated polymer materal has also met the demand of medical science and agricultural and other side, becomes the focus of Recent study.Degradable macromolecule is according to the difference of material source, and it is mainly divided into natural macromolecular material, synthesized polymer material and mix type macromolecular material.
Biodegradable medical macromolecular materials as for diagnosing, the material for the treatment of and neomorph, be widely used in recent years medicine and control the fields such as release vehicle, its research occupies very important position in fields such as biotechnology, life sciences and medical science.
Microgranule is the granule of 1 to 1000 micron of diameter.Preparing the most popular method of biodegradable microgranule is chemical precipitation method, phase separation method, spray drying method and solvent evaporated method.Wherein phase separation method, spray drying method and solvent evaporated method conventionally have adding of organic solvent in implementation procedure, may cause pollution in various degree to environment, and operation relative complex, and the response time is slightly long.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of preparation method of sodium alginate-calcium carbonate hybrid granule, raw material used in the present invention is cheap and easy to get, there is good biodegradability and biocompatibility, there is application and do the potentiality that follow-up related experiment is analyzed.
The preparation method of a kind of sodium alginate-calcium carbonate hybrid granule of the present invention, comprising:
(1) under 70-80 ℃ of condition, sodium alginate is added in distilled water, obtain sodium alginate soln;
(2) at ambient temperature, prepare respectively sodium carbonate, calcium chloride solution;
(3) sodium alginate soln is added in sodium carbonate liquor, stir, obtain mixed solution;
(4) calcium chloride solution is added rapidly in above-mentioned mixed solution, stir, centrifugal, dry, obtain sodium alginate-calcium carbonate hybrid granule, wherein sodium alginate soln, sodium carbonate liquor, the volume ratio of calcium chloride solution is 1:1:1.
In described step (1), the concentration of sodium alginate soln is 0.5-1g/L.
In described step (2), the concentration of sodium carbonate liquor is 0.05-0.1mol/L, and the concentration of calcium chloride solution is 0.05-0.1mol/L, and the solvent of solution is distilled water.
In described step (3), stir speed (S.S.) is 110-120r/min, and mixing time is 5-10min.
In described step (4), stir speed (S.S.) is 110-120r/min, and mixing time is 0.5-1h.
In described step (4), centrifugation rate is 8000-10000r/min, and centrifugation time is 5-30min.
In described step (4), baking temperature is 45-55 ℃.
What in described step (4), make has good application prospect containing carboxyl family macromolecule micron particle in the fields such as biomedical and biological engineering, is suitable for as pharmaceutical carrier, can be used for the preparation of wound dressing.
Chemical precipitation method be take water conventionally as solvent, belongs to environmentally friendly, and easy and simple to handle, and the response time is short.This patent adopts chemical precipitation method, and whole process all be take distilled water as solvent, has synthesized calcium carbonate-sodium alginate hydridization granule of 5 microns of left and right of diameter, and preparation method is cheap, and is green process.
beneficial effect
(1) whole preparation process of the present invention solvent used is distilled water, and the adding of organic solvent-free, environmentally safe, belongs to environmentally friendly production process;
(2) raw material used in the present invention is cheap and easy to get, has good biodegradability and biocompatibility, has application and does the potentiality that follow-up related experiment is analyzed.
Accompanying drawing explanation
Fig. 1 is the scanning electron microscope (SEM) photograph of the resulting calcium carbonate-sodium alginate of embodiment 1 hydridization granule;
Fig. 2 is the scanning electron microscope of the resulting calcium carbonate-sodium alginate of embodiment 2 hydridization granule;
Fig. 3 is the infrared spectrogram of the resulting calcium carbonate-sodium alginate of embodiment 1 hydridization granule;
Fig. 4 is that the resulting load of embodiment 2 has diclofenac sodium medicine, and the medicine of diclofenac sodium is controlled to release graphics.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
Under (1) 70 ℃ of condition, 5g sodium alginate is dissolved in 1000mL distilled water, obtains 0.5g/L sodium alginate soln;
(2) take in the volumetric flask that 10.6g natrium carbonicum calcinatum adds 1L, add distilled water, be mixed with the sodium carbonate liquor of 0.1mol/L;
(3) take in the volumetric flask that 11.1g anhydrous calcium chloride adds 1L, add distilled water, be mixed with the calcium chloride solution of 0.1mol/L;
(4) get sodium alginate soln 20mL described in (1), join in 100ml beaker, get sodium carbonate liquor described in same volume (2) simultaneously and add in beaker, under the rotating speed of 110r/min, stir ten minutes;
(5) get 20ml(3) described in calcium chloride solution, pour into rapidly described in (4) in mixed liquor, under same rotational speed, stir one hour;
(6) final resulting mixed liquor is put into centrifuge, under the rotating speed of 8000r/min centrifugal ten minutes, gained granule is put into 50 ℃ of thermostatic drying chambers dry.
Embodiment 2
(1) take 0.1g diclofenac sodium drug powder and add in the volumetric flask of 100ml, add distilled water, be mixed with the diclofenac sodium water solution of 1mg/ml;
(2) get sodium alginate soln 10mL described in embodiment 1, join in 100ml beaker, get sodium carbonate liquor described in same volume embodiment 1 simultaneously and add in beaker, under the rotating speed of 110r/min, stir ten minutes;
(3) get calcium chloride solution 10ml described in embodiment 1, join in 50ml beaker, get 10ml diclofenac sodium solution phase simultaneously and should join in the beaker that calcium chloride solution is housed, under the rotating speed of 110r/min, stir ten minutes, make it mix homogeneously;
(4) mixed liquor in beaker described in (3) is poured into rapidly in the beaker in (2), under the rotating speed of 110r/min, stir one hour;
(5) final resulting mixed liquor is put into centrifuge, under the rotating speed of 8000r/min centrifugal ten minutes, gained granule is put into 50 ℃ of thermostatic drying chambers dry, weigh the quality of dried powder, and get the centrifugal supernatant obtaining, with ultraviolet spectrophotometer, survey its absorbance, obtain the quality of free diclofenac sodium in solution, thereby calculate the quality of granule packaging medicine.
(6) the hydridization granule 12.2mg that includes 0.423mg diclofenac sodium medicine being put into pH value is to carry out external slow release experiment in 6.8 PBS buffer, obtains the external accumulative total release rate (data are as following table) of diclofenac sodium.
Claims (7)
1. a preparation method for sodium alginate-calcium carbonate hybrid granule, comprising:
(1) under 70-80 ℃ of condition, sodium alginate is added in distilled water, obtain sodium alginate soln;
(2) at ambient temperature, prepare respectively sodium carbonate, calcium chloride solution;
(3) sodium alginate soln is added in sodium carbonate liquor, stir, obtain mixed solution;
(4) calcium chloride solution is added in above-mentioned mixed solution, stir, centrifugal, dry, obtain sodium alginate-calcium carbonate hybrid granule, wherein sodium alginate soln, sodium carbonate liquor, the volume ratio of calcium chloride solution is 1:1:1.
2. the preparation method of a kind of sodium alginate-calcium carbonate hybrid granule according to claim 1, is characterized in that: in described step (1), the concentration of sodium alginate soln is 0.5-1g/L.
3. the preparation method of a kind of sodium alginate-calcium carbonate hybrid granule according to claim 1, it is characterized in that: in described step (2), the concentration of sodium carbonate liquor is 0.05-0.1mol/L, the concentration of calcium chloride solution is 0.05-0.1mol/L, and the solvent of solution is distilled water.
4. the preparation method of a kind of sodium alginate-calcium carbonate hybrid granule according to claim 1, is characterized in that: in described step (3), stir speed (S.S.) is 110-120r/min, and mixing time is 5-10min.
5. the preparation method of a kind of sodium alginate-calcium carbonate hybrid granule according to claim 1, is characterized in that: in described step (4), stir speed (S.S.) is 110-120r/min, and mixing time is 0.5-1h.
6. the preparation method of a kind of sodium alginate-calcium carbonate hybrid granule according to claim 1, is characterized in that: in described step (4), centrifugation rate is 8000-10000r/min, and centrifugation time is 5-30min.
7. the preparation method of a kind of sodium alginate-calcium carbonate hybrid granule according to claim 1, is characterized in that: in described step (4), baking temperature is 45-55 ℃.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105854026A (en) * | 2016-04-06 | 2016-08-17 | 温州生物材料与工程研究所 | Basic protein entrapment vector, preparation method of entrapment vector and entrapment method |
| CN106420631A (en) * | 2016-11-17 | 2017-02-22 | 青岛悦海医药科技有限公司 | Preparation method of micro-pore sodium alginate-starch composite particles |
| CN108392672A (en) * | 2018-03-23 | 2018-08-14 | 曹朗翘 | Radix Notoginseng seaweed gel dressing and preparation method thereof |
| KR20210088934A (en) * | 2020-01-07 | 2021-07-15 | 주식회사 테라시온 바이오메디칼 | Micro-beads and Method for producing thereof, Hemostatic dressings including the same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286155A (en) * | 2011-05-30 | 2011-12-21 | 武汉大学 | Preparation method of sodium alginate-calcium carbonate hybridized micron particles |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102286155A (en) * | 2011-05-30 | 2011-12-21 | 武汉大学 | Preparation method of sodium alginate-calcium carbonate hybridized micron particles |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105854026A (en) * | 2016-04-06 | 2016-08-17 | 温州生物材料与工程研究所 | Basic protein entrapment vector, preparation method of entrapment vector and entrapment method |
| CN106420631A (en) * | 2016-11-17 | 2017-02-22 | 青岛悦海医药科技有限公司 | Preparation method of micro-pore sodium alginate-starch composite particles |
| CN106420631B (en) * | 2016-11-17 | 2019-03-22 | 青岛悦海医药科技有限公司 | A kind of preparation method of micropore sodium alginate-starch composite particles |
| CN108392672A (en) * | 2018-03-23 | 2018-08-14 | 曹朗翘 | Radix Notoginseng seaweed gel dressing and preparation method thereof |
| KR20210088934A (en) * | 2020-01-07 | 2021-07-15 | 주식회사 테라시온 바이오메디칼 | Micro-beads and Method for producing thereof, Hemostatic dressings including the same |
| KR102302405B1 (en) | 2020-01-07 | 2021-09-16 | 주식회사 테라시온 바이오메디칼 | Micro-beads and Method for producing thereof, Hemostatic dressings including the same |
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